Carg19723 (gene) Silver-seed gourd

NameCarg19723
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionGDSL esterase/lipase
LocationCucurbita_argyrosperma_scaffold_133 : 346537 .. 352349 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTTTCTTTTTTTCGCGGGCCGAAGCCTGGTGCTCGCGCCTGTACAACCTCAGGAAACTTCAATTTCACGACTAGACTCGAATGCTTGTAAGATCGGTGTGCAAGAGATTGATAAGAGCCCATTTTCGTTCTCGAAGGGTTGATCGGAGCTTCGAAATAGCAGACCATGAGGCCTGAGATCGTTCTATTTGGGGATTCTTTGACTGCGCAATCCTTCGGCTCAGGCGGATGGGGAGCTGCGCTTGCTGACACTTACTCCCGGAAGGTGGATTCTCTTTTCTTTCTTTCTTTAATTTGTGTTTTCGTTCAAGTTTTTGAAGTTTTTGTGTTCGTAAGTGGGTGAGTTTGACTAATTTATAGGCCGATGTCCTAGTTCGTGGCTACGGCGGATACAATACTCGATGGGCTTTGTTTTTACTTCATCACATTTTTCCATTGGTATGTGTTATAGTGCGCTTTTTGTTCCTTCTCTACTTTGATTTTGAGGACTTTGTTTTTACTTGAATTTGTTTCGTTATGTTCAGTAAGTGATCTTGGTGATTATTCGCATTTTAGTACTTTTTGGGCGTTTTTTGTAATGCAACCTATCATTGTAGCTATGTTGTGGGAATTGTGCCCGTTTCTGATTCTTACATTTGATTTCAATTTAATTGAGGAAAAATTGGAAAGTTTTGCTCAATGGGGCTAAACTTACGGGGAGATCACCCAATGAACTCAAAGATGTGATTGAACAACAAACCAAACACATTGTTTGAAAGATTTTGGAATATTGTTGTACGTTAATGATGTTGTACACGAATATGTTTGGGAGTCTAAATACGTACCTGGTGGGATGTAACAGCCCAAGCCTACCACTAGCAGATGCAGTCCTCTTTAAGTTTTTCCTTTCGGATTTCTCCTTAAGGTTTTTTAAAACGCATATGCTAGGGAGAGGTTTTCACACTCTTATAAAGAATACTTCGTTCTCCTCTCCAACCGATGTGGGATCTCCCCTGGGATGTTGTGCAGAATTCTTGTGGTAAAGAGCCCATTATAGTTGAATTATTTGGCCCCTAGATACGTAGGATGCCGGGTTTAGTGGCCCATAATTGACCTTCCTTCACCAAAAAGCTTAATCTCTTGATGCTCAGCTCACAAGGAGTGCCTCTCGAATTGGGGGTTTTCAACCTTGCCTCATTGCAAAGTTTGTGTTTTCATGCCCCTTTGAGACCCTAAAAGAGGAACCAGTAGATATTCTTTGTACTAGCTTACACAGTCATGGGTAGAAACATCTTTGTAGGCTTCTGAAAAATGATGTTTCTTGTATATTTGCATTCCAAATAATTTGCATCACGTGTAATGCAGGAAATCCCAAAGGCCCCCATTGTTGTGACAATTTTCTTTGGGGCCAATGATGCAGCTATTTTTGGGAGAACAAGTGAGAGACAACACGTACCTCTTGACGAGTACAAGGATAATCTTAAAAAGATGGTAAATCATTTGAAGGTACTGACTTCTTTCTTCACTGTTCGAAATCTTTTCGAGTATACTGTGCTAATATTCATTTTGAAGATTTAAATGACTGTAAGACATTAGTCCATCACTTTTTTTTTTCATCTGTTTATAAGTTCCCCATTGACAAGGTAGTTGCTTCAGAAAGAGAGATGTTAAAAAATATATTTATGAATTAGTTCAAAGCAGGAGTACAACTTTCAATTACTGGAAATGCTTTAATAGACTTTCATATTTTCTTTTGATTTGTTCTGTTGGCCATTAATTTGGTACATTCTGAAATTGTTGATGCCTGATGGATTGGAGAGGACAGAGCCACCCTCAACTATTTCGTGCTCACGAGATCGTTAAAACACTTCAGACCATACATACTTAGATATTTCATTTAAGGGTGTATTGAAGACTGAATTAAGTGCACGTTCGTCGAGATTAGTATGAATGGTGATACTAAGAAGAAAGTCTCCATTGTACTAAAAAAAGTTCTGGCTATTTAGCTATTTGGATATGCCCCGGGAACTAATCTTAGGAGTTTAAACATTTTGGAATGGAGTTATAGAAAGGGCGAGATCTACCCTTCTAAGTGGCAGACATGTTACTTCTAAATAGGTTAGATTAGTCTAGTCACTAGTGCATTGGGTAACCACCGTTAATTGGCCCAGTTGTCTTTGACATTTGGTAAACTCGTAATTTTTAGATAATAAGTTCAAACCATGGTGACCTCCTATCTCGATAATAAACATCCTATGAGTTTTTCAAAATCAAATATCGTAGGATGAGACAGTCCACTTAGAGAGAGATTTATTGACATTTGCTTGCTGCCCAAAAAACCTAAGACTATTAGAACGAAAAGGTCTAGTGCAAACTGCTAGCGATCTGAGAAACGTTCCGACTCACTTCCTTTCTCTATTTAAAACTTTTATAAGGTAGGTAAACAAGTTAGATTGGAATGTTAGGAACTTCTTATGGGTTTGTAGTTTGAATGTGAGAGGGTTAATGAAAGTGGGCAACCACTTGTCTGACCTGAAAGTGGTCCTCTTCCTGGGAAGAAGGTTGAATGAAATGGGAGAGTTAAACCTTCGATCAGACTTCTAGGAAGACGTCCAGCTTGCAGATTGCTCATATTCTTTGAAAATGTGATTTTGCATTAGTCTTTTGAAATTGTTTCTTTGAGGCGTTTTGGCTCATGCTTTGTCAAATCTCGGGATTGTAGAGAGATGATCAAGGAGCTCTTCTTCAGTCGCTCTTTCGTGAGAAATGAAGGTTCTTGTGAGCTTCGGTAGCGAAGTTATTTTGTAACTATCCTTTAGGGGTCGTTTGGTATAAGGTTTAAAGGTTTATAACGGTGTTTAACTCTATTAGAGAATGTTTTTCAGATTCTTATAGGAATAAATTATTTCAATACTTCAATGTTTCCCTTCACTTGTGGGCTTGAAAATTTGTAGAAGGCTCCAACAAGTAGAAATTAATATTAACTGGGGGAGGAAATGAAATAACAAAAGTTTGAACACACGACTTTTGTTATGATACCATGTTAAATCACCACTCAACCCTAAAGTTTAAGTGATAAATTACGATAAATTTACTTATTTCAACACTTCAACCAGGGTTATAGCTAATTATGGGTGCTGTGAAATGAAAGAAATCATGTGGCCTTTAATGAGCTCAATTCAAAGGTTTCAAAAGAGTCGAGTTTCTTTTTCGTTCCTCTGGTGCACTCTTCCCAGTTTCTTATGTAATTGTAGCACTCTTTTGATTCAACCCTGTTGGATTATTTTCTTGTATCCACTTCTCCCCCATTTACATCTCATTGCATTGTGGCATTTTGGTTGCAGGAGTACTCCCCAACCACGTTGGTGATACTTATTACTCCTCCTCCTGTCGACGAGGACGGGCGTAATGAATATGCACGGTATGACTTTTTGTTTCTAATCGCTGCTCAAACTTTTTAGCCTTTTTCTGGTGTAGTGGTACGTACAACAGTCTATATATTCCATCTTTCTACTTTTCCTTCAACTAGTTGTTAGGATCGCACAACACACACACTATGAACACAAAGAACAAGAGAAGGAAAATGCAAGGAAAAATATTAGCTAGATGTTTATATTGATGACTTCAAATATGAACCGTAAGAGAGAATACAAAGTGTACAGAAAGTATGTTTTCAAAGTATTGCCCAAGGGAATTATTTATGGTTCAGTTTACTATATATTATATATAACTTTTCTCTAGGTATAGTCATCTACTGTATATAAGTATCTACCCTATATAATATAGCCTCTTATACTAGACATATAGCCTCTTATACTAGACATAGTTGTTTACTATATATATCATTTACTTTACATTTACTTTACATGTTATTTATCCTATATATAACTTTACCTGTATAATAGTAGATGACTCCATAACCTAACACTAGTTTCATATTAACATATAAGTTATATATCGAGTATTACCAAAATATACTTTTTCATTCTTGAACCTAATGTGTAAATGTCTGAACCTGTTGAGTCCAAATGTACTCTATGCCCCTATTACGCCCTCGCTGCAGATCATCACCGATATCGGCATTTAAAGGTGGTCTAAGAACACAATCTTCAGACCAAGACAACTATACTTATTTTAAACTCTCTTCAGCAAGTTGTCTTATTTTAAGTTTCATCAATATGCTGAAGAGAGATTTTTATCCTACTCAGACCAAGACAACTTTACTTGTTCAAAAACCAATTAACGTGAAGCAGGTCTTTTCTTGATTGTGCAAAAATAACTTGTGTCAATCTATCAGATCTCTTTATGGTGATAAAGCAAGGGAATTGCCTGAGAGATCAAATGAAGTAACTGGACTTTACGCGAAACAATGCCTTGAACTAGCTAAGGAAATGGGTCTTCTCGCCATTGATCTATGGTCCAAGTTGCAGGAAACAGAGGGCTGGCAGAAAAAATTTCTCAGGTCAGTGAATCCAACTCCTAGTTCATAAGTTTTACTCATTTTTGAAATATGGCTGCTGTTATCTTTAATGTTATATGTCCGACTACACTTAAAATGTTATTCCGAATCAACCCTTAGTCGCATGAATCAAATTTGATATCTACGAGTGAGAGGGATTGTTAATGAAGATTATAAATAATTCAATGTTCTTCAATGTAATATCCCACATTGGTTGGAGAGGAGAACGAAACAATCTTTAAAAGGATGTGGAAACCTCTCCCTAGCAGACGCGTTTTAAATCCTTGAGGGGAAGCTCAAAGAGGACAATATTTGCTACTGGTGGGCTTGGGCCGTTACAAATAGTATAAGAGCCAGATACTGGGCGATGTGTCAATGAGGAGGCTGTTCCCCGAAGGGGGTAGACACGAGGCAGTGTGTCAGTAAGAACGCTGGGCCCCAAAGGGGGATGGATTTGGTGGGGATCCCACATCGATTTGAGAAAGGAACCAGTGCCAGCAAGGACTCTAGGCCTTGAAGGGGGGGTGAATTGTGATATCCCACATTGGTTGGGGAGGAGAACGAAACACCCTTTAAAAGCGTGTAGAAACCTCTCCCTAGCAGACGTGTTTTAAAGCCTTGAGGGGAAGCCCGAAAGGGAAAGTCTAAAGAGGACAATATCTGCTAGCAGTGGATGGGCCGTTACATTCAACTCAACTTTCAAGTGATATTAATTGTTGTGCGTTGAAATTTGTGAGTTAATGTGAGATCCCACATCAGTTGGAGAGGAGAACGAAGCATTCTTTAGAAGGACATGAAAACCTCTCCCTAGTAGACACGTTTTAAAACCGTGAGGCTGACGATGATACGTAACAGGGCCAAAGTAGACAATATCTACTAGCGGTGGGCTTAGGCTGTTACAGTTGAACTGTTCTTATTCTAACTCAATAGCTTAAACTCTAAATTGAACGGTCACACAAGCATAGCGAAGAAGTAGAATTCCTTGAACGAACGTCCACCCCACCCCCGTTTCGTATGAACTTAGTAGTGAGGCATTGTTTGTGTTTTACATGTAGGGATGGATTGCATTTCACGCCAGAAGGCAACGGAATTCTGCACCAAGAACTAGAGAAAGTACTGAATGAAACATCAGTTTCTGCTGCAAAATTGCCTCTTGATTTCCCACATCACTCAGAAATCGATGGAAAAAGCCCCGAAAAGGCCTTCCAATATCTTTCTTTGTGATATACTTTCATCATCATATCATATCTTGTTATGGAAGAAAGCTTTATTTGTATTTGCATTTGATTGTAAACCATAATTTTCTGTCTTCCATTTGGTATCATCTTGTTTGATGAGTATGAATATTAAATATTAGAGACTAAAATGTGTTTA

mRNA sequence

TATTTTCTTTTTTTCGCGGGCCGAAGCCTGGTGCTCGCGCCTGTACAACCTCAGGAAACTTCAATTTCACGACTAGACTCGAATGCTTGTAAGATCGGTGTGCAAGAGATTGATAAGAGCCCATTTTCGTTCTCGAAGGGTTGATCGGAGCTTCGAAATAGCAGACCATGAGGCCTGAGATCGTTCTATTTGGGGATTCTTTGACTGCGCAATCCTTCGGCTCAGGCGGATGGGGAGCTGCGCTTGCTGACACTTACTCCCGGAAGGCCGATGTCCTAGTTCGTGGCTACGGCGGATACAATACTCGATGGGCTTTGTTTTTACTTCATCACATTTTTCCATTGGAAATCCCAAAGGCCCCCATTGTTGTGACAATTTTCTTTGGGGCCAATGATGCAGCTATTTTTGGGAGAACAAGTGAGAGACAACACGTACCTCTTGACGAGTACAAGGATAATCTTAAAAAGATGGTAAATCATTTGAAGGAGTACTCCCCAACCACGTTGGTGATACTTATTACTCCTCCTCCTGTCGACGAGGACGGGCGTAATGAATATGCACGATCTCTTTATGGTGATAAAGCAAGGGAATTGCCTGAGAGATCAAATGAAGTAACTGGACTTTACGCGAAACAATGCCTTGAACTAGCTAAGGAAATGGGTCTTCTCGCCATTGATCTATGGTCCAAGTTGCAGGAAACAGAGGGCTGGCAGAAAAAATTTCTCAGGGATGGATTGCATTTCACGCCAGAAGGCAACGGAATTCTGCACCAAGAACTAGAGAAAGTACTGAATGAAACATCAGTTTCTGCTGCAAAATTGCCTCTTGATTTCCCACATCACTCAGAAATCGATGGAAAAAGCCCCGAAAAGGCCTTCCAATATCTTTCTTTGTGATATACTTTCATCATCATATCATATCTTGTTATGGAAGAAAGCTTTATTTGTATTTGCATTTGATTGTAAACCATAATTTTCTGTCTTCCATTTGGTATCATCTTGTTTGATGAGTATGAATATTAAATATTAGAGACTAAAATGTGTTTA

Coding sequence (CDS)

ATGAGGCCTGAGATCGTTCTATTTGGGGATTCTTTGACTGCGCAATCCTTCGGCTCAGGCGGATGGGGAGCTGCGCTTGCTGACACTTACTCCCGGAAGGCCGATGTCCTAGTTCGTGGCTACGGCGGATACAATACTCGATGGGCTTTGTTTTTACTTCATCACATTTTTCCATTGGAAATCCCAAAGGCCCCCATTGTTGTGACAATTTTCTTTGGGGCCAATGATGCAGCTATTTTTGGGAGAACAAGTGAGAGACAACACGTACCTCTTGACGAGTACAAGGATAATCTTAAAAAGATGGTAAATCATTTGAAGGAGTACTCCCCAACCACGTTGGTGATACTTATTACTCCTCCTCCTGTCGACGAGGACGGGCGTAATGAATATGCACGATCTCTTTATGGTGATAAAGCAAGGGAATTGCCTGAGAGATCAAATGAAGTAACTGGACTTTACGCGAAACAATGCCTTGAACTAGCTAAGGAAATGGGTCTTCTCGCCATTGATCTATGGTCCAAGTTGCAGGAAACAGAGGGCTGGCAGAAAAAATTTCTCAGGGATGGATTGCATTTCACGCCAGAAGGCAACGGAATTCTGCACCAAGAACTAGAGAAAGTACTGAATGAAACATCAGTTTCTGCTGCAAAATTGCCTCTTGATTTCCCACATCACTCAGAAATCGATGGAAAAAGCCCCGAAAAGGCCTTCCAATATCTTTCTTTGTGA

Protein sequence

MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLEIPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPPPVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEGWQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYLSL
BLAST of Carg19723 vs. NCBI nr
Match: XP_023551925.1 (GDSL esterase/lipase At5g62930 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 498.8 bits (1283), Expect = 1.0e-137
Identity = 241/242 (99.59%), Postives = 242/242 (100.00%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLE 60
           MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL+
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
           IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG
Sbjct: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of Carg19723 vs. NCBI nr
Match: XP_022922525.1 (GDSL esterase/lipase At5g62930 [Cucurbita moschata])

HSP 1 Score: 495.4 bits (1274), Expect = 1.1e-136
Identity = 240/242 (99.17%), Postives = 241/242 (99.59%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLE 60
           MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL+
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
           IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG
Sbjct: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLHFTPEGN ILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNVILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of Carg19723 vs. NCBI nr
Match: XP_022985167.1 (GDSL esterase/lipase At5g62930 [Cucurbita maxima])

HSP 1 Score: 494.2 bits (1271), Expect = 2.5e-136
Identity = 238/242 (98.35%), Postives = 240/242 (99.17%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLE 60
           MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL+
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLD 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
           IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGL+AIDLWSKLQETEG
Sbjct: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLIAIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLHFTPEGNGILH ELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAF YL
Sbjct: 181 WQKKFLRDGLHFTPEGNGILHHELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFHYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of Carg19723 vs. NCBI nr
Match: XP_011652964.1 (PREDICTED: GDSL esterase/lipase At5g62930 isoform X2 [Cucumis sativus] >XP_011652966.1 PREDICTED: GDSL esterase/lipase At5g62930 isoform X2 [Cucumis sativus] >KGN52868.1 hypothetical protein Csa_4G004790 [Cucumis sativus])

HSP 1 Score: 469.2 bits (1206), Expect = 8.7e-129
Identity = 221/242 (91.32%), Postives = 236/242 (97.52%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLE 60
           MRPEIVLFGDS+TAQSFG GGWGAALADTYSRKADV+VRGYGGYNTRWALFLLHHIFPL 
Sbjct: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
            PKAPIVVTIFFGANDAA+ GRTSE+QHVPL+EYK NLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDE+GRNEYARSLYGDKARELPER+NEVTGLYAK+CLELAKE+GL AIDLWSK+QETEG
Sbjct: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLHFTPEGNG+LHQELEKVLNETSV+AAK+PLDFPHHS+IDGK+PEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of Carg19723 vs. NCBI nr
Match: XP_022149345.1 (GDSL esterase/lipase At5g62930 isoform X2 [Momordica charantia])

HSP 1 Score: 466.1 bits (1198), Expect = 7.4e-128
Identity = 222/242 (91.74%), Postives = 236/242 (97.52%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLE 60
           MRP+IVLFGDSLTAQSFGSGGWGAALADTYSRKADV+VRGYGGYN+RWALFLLHHIFPL+
Sbjct: 1   MRPQIVLFGDSLTAQSFGSGGWGAALADTYSRKADVVVRGYGGYNSRWALFLLHHIFPLD 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
           IPK PIVVTIFFGANDAAI GRTSERQHVPL+E+K+NLKKMVNHLKE SPTTLVILITPP
Sbjct: 61  IPKPPIVVTIFFGANDAAILGRTSERQHVPLEEFKNNLKKMVNHLKECSPTTLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDE+GRNEYARSLYGD+ARELPER+NEVTGLYA QCLELAKEMGLLAIDLWSKLQETEG
Sbjct: 121 PVDEEGRNEYARSLYGDEARELPERTNEVTGLYATQCLELAKEMGLLAIDLWSKLQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLH TPEGN I+HQELEKVLNETSVSAAKLPLDFPHHS+IDGK+PEKAF+YL
Sbjct: 181 WQKKFLRDGLHLTPEGNAIVHQELEKVLNETSVSAAKLPLDFPHHSKIDGKNPEKAFEYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of Carg19723 vs. TAIR10
Match: AT5G62930.1 (SGNH hydrolase-type esterase superfamily protein)

HSP 1 Score: 360.1 bits (923), Expect = 1.0e-99
Identity = 160/238 (67.23%), Postives = 197/238 (82.77%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLE 60
           MRPEIVLFGDS+TAQSF SGGWG+ALAD YSRKADV+VRGYGGYNTRWALFLLHHIFPL 
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLG 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
               P+  TIFFGANDAA+ GRTS+RQHVP++EY DN++K+V HLK+ SPT L++LITPP
Sbjct: 61  SSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVLITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           P+DE GR  YA S+YG+KA + PER+NE TG+YA+ C+ LA+E+GL  ++LWSK+QET  
Sbjct: 121 PIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQETND 180

Query: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQ 239
           WQKK+L DGLH TPEGNG++  E+ +V  E  +S  ++P DFPHHS IDGK+P KAF+
Sbjct: 181 WQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSPEEMPFDFPHHSHIDGKNPSKAFE 238

BLAST of Carg19723 vs. TAIR10
Match: AT5G45920.1 (SGNH hydrolase-type esterase superfamily protein)

HSP 1 Score: 240.0 bits (611), Expect = 1.6e-63
Identity = 118/240 (49.17%), Postives = 158/240 (65.83%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL- 60
           MR +I LFGDS+T +SF  GGWGA+LAD   RKAD+++RGY GYNTRWAL ++  +FP+ 
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 61  --EIPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILI 120
             +   +P  VT+FFGANDA +  R S  QHVPL EYK NL+ +V+ LK   P T +ILI
Sbjct: 62  EEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIILI 121

Query: 121 TPPPVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQE 180
           TPPP+DE+ R  Y    Y +    LPER+NEV GLYAK C+ +A+E  +   DLWSK+Q+
Sbjct: 122 TPPPIDEEARLRYP---YIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQ 181

Query: 181 TEGWQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAF 238
              WQ + L DGLH +  GN ++ +E+ K L E  + A  L +D P   ++D K P K+F
Sbjct: 182 IPNWQTECLWDGLHLSRVGNKVVFEEVAKKLKEEGIGAEDLAVDLPLIEDVDPKDPLKSF 238

BLAST of Carg19723 vs. TAIR10
Match: AT2G38180.1 (SGNH hydrolase-type esterase superfamily protein)

HSP 1 Score: 177.9 bits (450), Expect = 7.3e-45
Identity = 89/227 (39.21%), Postives = 139/227 (61.23%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLE 60
           +RP+IVLFG S+   SF   GWGA LAD YSR AD+++RGY G+N+R+AL +LH +FP +
Sbjct: 5   VRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQVFPKD 64

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
               P +V ++FG ND+     +    HVPL E+ +N++K+  HL   S  T VI +TPP
Sbjct: 65  AVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIFLTPP 124

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           P++E    +    ++GD    +  RSNE+   YA++ L L +E+ +  ID+W+ +Q+ + 
Sbjct: 125 PMNE----KQIEIVFGD---AIKGRSNELCRPYAEELLNLCREINVKGIDIWTAIQQQDD 184

Query: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLN----ETSVSAAKLPLDFP 224
           W      DG+HFT + + I+ +E+ KVL     + S+    LP++FP
Sbjct: 185 WLNSCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEFP 224

BLAST of Carg19723 vs. TAIR10
Match: AT3G11210.1 (SGNH hydrolase-type esterase superfamily protein)

HSP 1 Score: 167.9 bits (424), Expect = 7.5e-42
Identity = 86/232 (37.07%), Postives = 137/232 (59.05%), Query Frame = 0

Query: 2   RPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLEI 61
           RP+IVLFG S+   SFG GGWGA L++ Y+RKAD+++RGY G+N+  AL ++  +FP + 
Sbjct: 6   RPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQVFPKDA 65

Query: 62  PKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPPP 121
              P +V ++FG ND+     +    HVPL EY DN+KK+  HL+  S  T +I ++ PP
Sbjct: 66  AVQPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALHLQSLSDFTRIIFLSSPP 125

Query: 122 VDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEGW 181
           VDE  +    +S Y  +      R+N++   Y+  C+EL +E+GL  +DL+S  Q+ + W
Sbjct: 126 VDE-AKVRQNQSPYLSEV----IRTNDLCKTYSDACVELCQELGLEVVDLFSTFQKADDW 185

Query: 182 QKKFLRDGLHFTPEGNGILHQELEKVLNET----SVSAAKLPLDFPHHSEID 230
           +     DG+H + +G+ I+  E+ +V+ E     S+    +P +F   S  D
Sbjct: 186 KTVCFTDGIHLSAQGSKIVAGEILRVVKEAEWHPSLHWKSMPTEFADDSPYD 232

BLAST of Carg19723 vs. Swiss-Prot
Match: sp|Q9FM04|GDL88_ARATH (GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2 SV=3)

HSP 1 Score: 360.1 bits (923), Expect = 1.9e-98
Identity = 160/238 (67.23%), Postives = 197/238 (82.77%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLE 60
           MRPEIVLFGDS+TAQSF SGGWG+ALAD YSRKADV+VRGYGGYNTRWALFLLHHIFPL 
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPLG 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
               P+  TIFFGANDAA+ GRTS+RQHVP++EY DN++K+V HLK+ SPT L++LITPP
Sbjct: 61  SSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQHLKKCSPTMLIVLITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           P+DE GR  YA S+YG+KA + PER+NE TG+YA+ C+ LA+E+GL  ++LWSK+QET  
Sbjct: 121 PIDEAGRQSYAESIYGEKAMKEPERTNETTGVYAQHCVALAEELGLRCVNLWSKMQETND 180

Query: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQ 239
           WQKK+L DGLH TPEGNG++  E+ +V  E  +S  ++P DFPHHS IDGK+P KAF+
Sbjct: 181 WQKKYLSDGLHLTPEGNGVVFDEVSRVFREAWLSPEEMPFDFPHHSHIDGKNPSKAFE 238

BLAST of Carg19723 vs. Swiss-Prot
Match: sp|Q6NMR9|GDL84_ARATH (GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 2.8e-62
Identity = 118/240 (49.17%), Postives = 158/240 (65.83%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL- 60
           MR +I LFGDS+T +SF  GGWGA+LAD   RKAD+++RGY GYNTRWAL ++  +FP+ 
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 61  --EIPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILI 120
             +   +P  VT+FFGANDA +  R S  QHVPL EYK NL+ +V+ LK   P T +ILI
Sbjct: 62  EEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVSFLKNRWPQTAIILI 121

Query: 121 TPPPVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQE 180
           TPPP+DE+ R  Y    Y +    LPER+NEV GLYAK C+ +A+E  +   DLWSK+Q+
Sbjct: 122 TPPPIDEEARLRYP---YIENTTGLPERTNEVAGLYAKACIAVAEECQISVTDLWSKMQQ 181

Query: 181 TEGWQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAF 238
              WQ + L DGLH +  GN ++ +E+ K L E  + A  L +D P   ++D K P K+F
Sbjct: 182 IPNWQTECLWDGLHLSRVGNKVVFEEVAKKLKEEGIGAEDLAVDLPLIEDVDPKDPLKSF 238

BLAST of Carg19723 vs. Swiss-Prot
Match: sp|O80443|GDL46_ARATH (GDSL esterase/lipase At2g38180 OS=Arabidopsis thaliana OX=3702 GN=At2g38180 PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.3e-43
Identity = 89/227 (39.21%), Postives = 139/227 (61.23%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLE 60
           +RP+IVLFG S+   SF   GWGA LAD YSR AD+++RGY G+N+R+AL +LH +FP +
Sbjct: 5   VRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQVFPKD 64

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
               P +V ++FG ND+     +    HVPL E+ +N++K+  HL   S  T VI +TPP
Sbjct: 65  AVIQPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGEHLLSLSDKTRVIFLTPP 124

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           P++E    +    ++GD    +  RSNE+   YA++ L L +E+ +  ID+W+ +Q+ + 
Sbjct: 125 PMNE----KQIEIVFGD---AIKGRSNELCRPYAEELLNLCREINVKGIDIWTAIQQQDD 184

Query: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLN----ETSVSAAKLPLDFP 224
           W      DG+HFT + + I+ +E+ KVL     + S+    LP++FP
Sbjct: 185 WLNSCFTDGIHFTAKASEIVVKEILKVLRGADWKPSLYWKSLPVEFP 224

BLAST of Carg19723 vs. Swiss-Prot
Match: sp|Q2TAA2|IAH1_HUMAN (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Homo sapiens OX=9606 GN=IAH1 PE=1 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 5.5e-42
Identity = 100/237 (42.19%), Postives = 139/237 (58.65%), Query Frame = 0

Query: 3   PEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIF----P 62
           P ++LFGDS+T  SF  GGWGA+LAD   RK DVL RG+ GYNTRWA  +L  +      
Sbjct: 16  PRLLLFGDSITQFSFQQGGWGASLADRLVRKCDVLNRGFSGYNTRWAKIILPRLIRKGNS 75

Query: 63  LEIPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYS-PTTLVILI 122
           L+I   P+ VTIFFGAND+A+    + +QH+PL+EY  NLK MV +LK    P   VILI
Sbjct: 76  LDI---PVAVTIFFGANDSALKDE-NPKQHIPLEEYAANLKSMVQYLKSVDIPENRVILI 135

Query: 123 TPPPVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQE 182
           TP P+ E    E    + G K   L    N V G YA  CL++A++ G   +DLW+ +Q+
Sbjct: 136 TPTPLCETAWEEQC-IIQGCKLNRL----NSVVGEYANACLQVAQDCGTDVLDLWTLMQD 195

Query: 183 TEGWQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPE 235
           ++ +   +L DGLH +P+GN  L   L  ++ +     + LPL  P+  ++    PE
Sbjct: 196 SQDF-SSYLSDGLHLSPKGNEFLFSHLWPLIEK---KVSSLPLLLPYWRDVAEAKPE 239

BLAST of Carg19723 vs. Swiss-Prot
Match: sp|Q9DB29|IAH1_MOUSE (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Mus musculus OX=10090 GN=Iah1 PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.0e-40
Identity = 101/238 (42.44%), Postives = 135/238 (56.72%), Query Frame = 0

Query: 3   PEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLEIP 62
           P ++LFGDS+T  SF  GGWG+ LAD   RK DVL RG+ GYNTRWA  +L    P  I 
Sbjct: 16  PRVLLFGDSITQFSFQQGGWGSLLADRLVRKCDVLNRGFSGYNTRWAKIIL----PRLIR 75

Query: 63  KA-----PIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYS-PTTLVIL 122
           K      P+ VTIFFGAND+++    + +QHVPLDEY  NL+ MV +L+    P   VIL
Sbjct: 76  KGPGMENPVAVTIFFGANDSSLKDE-NPKQHVPLDEYSANLRDMVQYLRSVDVPRERVIL 135

Query: 123 ITPPPVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQ 182
           ITPPP+ E    E    L G K   L    N V G YA  CL++A++ G   +DLW+ +Q
Sbjct: 136 ITPPPLCE-AAWEKECVLKGCKLNRL----NSVVGEYANACLQVARDCGTDVLDLWTLMQ 195

Query: 183 ETEGWQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPE 235
           +       +L DGLH +P GN  L   L  +L++     + LP   P+  +++   PE
Sbjct: 196 KDSQDFSSYLSDGLHLSPMGNEFLFLNLCPLLDK---KVSSLPWLLPYWKDVEEAKPE 240

BLAST of Carg19723 vs. TrEMBL
Match: tr|A0A0A0KTH4|A0A0A0KTH4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G004790 PE=4 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 5.8e-129
Identity = 221/242 (91.32%), Postives = 236/242 (97.52%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLE 60
           MRPEIVLFGDS+TAQSFG GGWGAALADTYSRKADV+VRGYGGYNTRWALFLLHHIFPL 
Sbjct: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPLN 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
            PKAPIVVTIFFGANDAA+ GRTSE+QHVPL+EYK NLKKMVNHLKEYSPTTLVILITPP
Sbjct: 61  SPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVNHLKEYSPTTLVILITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDE+GRNEYARSLYGDKARELPER+NEVTGLYAK+CLELAKE+GL AIDLWSK+QETEG
Sbjct: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKECLELAKEIGLHAIDLWSKMQETEG 180

Query: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLHFTPEGNG+LHQELEKVLNETSV+AAK+PLDFPHHS+IDGK+PEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLNETSVAAAKMPLDFPHHSKIDGKNPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of Carg19723 vs. TrEMBL
Match: tr|A0A1S3BZF4|A0A1S3BZF4_CUCME (GDSL esterase/lipase At5g62930 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494719 PE=4 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 1.9e-127
Identity = 219/242 (90.50%), Postives = 234/242 (96.69%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLE 60
           MRPEIVLFGDS+TAQSFG GGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL 
Sbjct: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLN 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
            PKAPIVVTIFFGANDAAI GRTSERQHVPL+EYK NLKKMVNHLKEYSPTTLV+LITPP
Sbjct: 61  SPKAPIVVTIFFGANDAAILGRTSERQHVPLEEYKYNLKKMVNHLKEYSPTTLVVLITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDE+GRNEYARSLYGDKARELPER+NEVTGLYAK+CLELAKE+GL +IDLWSK+QET+G
Sbjct: 121 PVDEEGRNEYARSLYGDKARELPERTNEVTGLYAKKCLELAKEIGLHSIDLWSKMQETDG 180

Query: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYL 240
           WQKKFLRDGLHFTPEGNG+LHQELEKVL ETSV+A K+PLDFPHHS+IDGK+PEKAFQYL
Sbjct: 181 WQKKFLRDGLHFTPEGNGVLHQELEKVLKETSVAAEKMPLDFPHHSKIDGKNPEKAFQYL 240

Query: 241 SL 243
           SL
Sbjct: 241 SL 242

BLAST of Carg19723 vs. TrEMBL
Match: tr|A0A1S3BYB4|A0A1S3BYB4_CUCME (GDSL esterase/lipase At5g62930 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494719 PE=4 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 1.1e-119
Identity = 219/298 (73.49%), Postives = 234/298 (78.52%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL- 60
           MRPEIVLFGDS+TAQSFG GGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL 
Sbjct: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLN 60

Query: 61  -------------------------------------------------------EIPKA 120
                                                                    PKA
Sbjct: 61  PDLLEHCIIGSLDTLDAGFSGPKPICLHQNAPLLEAQFTNHPSCSRSFQPCFHPKNSPKA 120

Query: 121 PIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPPPVDE 180
           PIVVTIFFGANDAAI GRTSERQHVPL+EYK NLKKMVNHLKEYSPTTLV+LITPPPVDE
Sbjct: 121 PIVVTIFFGANDAAILGRTSERQHVPLEEYKYNLKKMVNHLKEYSPTTLVVLITPPPVDE 180

Query: 181 DGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEGWQKK 240
           +GRNEYARSLYGDKARELPER+NEVTGLYAK+CLELAKE+GL +IDLWSK+QET+GWQKK
Sbjct: 181 EGRNEYARSLYGDKARELPERTNEVTGLYAKKCLELAKEIGLHSIDLWSKMQETDGWQKK 240

Query: 241 FLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAFQYLSL 243
           FLRDGLHFTPEGNG+LHQELEKVL ETSV+A K+PLDFPHHS+IDGK+PEKAFQYLSL
Sbjct: 241 FLRDGLHFTPEGNGVLHQELEKVLKETSVAAEKMPLDFPHHSKIDGKNPEKAFQYLSL 298

BLAST of Carg19723 vs. TrEMBL
Match: tr|A0A2P5CF24|A0A2P5CF24_9ROSA (Lipase OS=Trema orientalis OX=63057 GN=TorRG33x02_287230 PE=4 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 1.4e-106
Identity = 182/237 (76.79%), Postives = 207/237 (87.34%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLE 60
           MRP+I LFGDS+T QSF  GGWGAALA+ YSRKADVLVRGYGGYNTRWALFLLHH+FPL 
Sbjct: 1   MRPQIALFGDSITEQSFRPGGWGAALANNYSRKADVLVRGYGGYNTRWALFLLHHLFPLN 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
               P  VTIFFGANDAAI GR SERQHVP DEYK+NL+KMV HLK  SPT LV+LITPP
Sbjct: 61  SANPPAAVTIFFGANDAAILGRQSERQHVPTDEYKENLRKMVLHLKGCSPTVLVVLITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDE+GRN YARSLYG+KARELPER+NEVTGLYAKQC+ELAKEMGL +IDLWSK+QET+G
Sbjct: 121 PVDEEGRNAYARSLYGEKARELPERTNEVTGLYAKQCIELAKEMGLRSIDLWSKMQETQG 180

Query: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAF 238
           WQKKFL DGLH TP+GN ++HQE+ KV +E+ +S  ++P DFPHHS+IDGK+PEKAF
Sbjct: 181 WQKKFLSDGLHLTPDGNAVVHQEVTKVFSESWLSPVEMPFDFPHHSQIDGKNPEKAF 237

BLAST of Carg19723 vs. TrEMBL
Match: tr|A0A2P5AF70|A0A2P5AF70_PARAD (Lipase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_338450 PE=4 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 2.4e-106
Identity = 181/237 (76.37%), Postives = 208/237 (87.76%), Query Frame = 0

Query: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPLE 60
           MRP+I LFGDS+T QSF  GGWGAALA+ YSRKADVLVRGYGGYNTRWA FLLHH+FPL 
Sbjct: 1   MRPQIALFGDSITEQSFRPGGWGAALANNYSRKADVLVRGYGGYNTRWAFFLLHHLFPLN 60

Query: 61  IPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVNHLKEYSPTTLVILITPP 120
               P  VTIFFGANDAAI GR SERQHVP+DEYK+NL++MV HLK  SPT LV+LITPP
Sbjct: 61  SANPPAAVTIFFGANDAAILGRQSERQHVPIDEYKENLRRMVLHLKGCSPTVLVVLITPP 120

Query: 121 PVDEDGRNEYARSLYGDKARELPERSNEVTGLYAKQCLELAKEMGLLAIDLWSKLQETEG 180
           PVDE+GRN YARSLYG+KARELPER+NEVTGLYAKQC+ELAKEMGL +IDLWSK+QET+G
Sbjct: 121 PVDEEGRNAYARSLYGEKARELPERTNEVTGLYAKQCIELAKEMGLRSIDLWSKMQETQG 180

Query: 181 WQKKFLRDGLHFTPEGNGILHQELEKVLNETSVSAAKLPLDFPHHSEIDGKSPEKAF 238
           WQKKFLRDGLH TP+GN ++HQE+ KV +E+ +S A++  DFPHHS+IDGK+PEKAF
Sbjct: 181 WQKKFLRDGLHLTPDGNAVVHQEVTKVFSESWLSPAEMAFDFPHHSQIDGKNPEKAF 237

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023551925.11.0e-13799.59GDSL esterase/lipase At5g62930 [Cucurbita pepo subsp. pepo][more]
XP_022922525.11.1e-13699.17GDSL esterase/lipase At5g62930 [Cucurbita moschata][more]
XP_022985167.12.5e-13698.35GDSL esterase/lipase At5g62930 [Cucurbita maxima][more]
XP_011652964.18.7e-12991.32PREDICTED: GDSL esterase/lipase At5g62930 isoform X2 [Cucumis sativus] >XP_01165... [more]
XP_022149345.17.4e-12891.74GDSL esterase/lipase At5g62930 isoform X2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G62930.11.0e-9967.23SGNH hydrolase-type esterase superfamily protein[more]
AT5G45920.11.6e-6349.17SGNH hydrolase-type esterase superfamily protein[more]
AT2G38180.17.3e-4539.21SGNH hydrolase-type esterase superfamily protein[more]
AT3G11210.17.5e-4237.07SGNH hydrolase-type esterase superfamily protein[more]
Match NameE-valueIdentityDescription
sp|Q9FM04|GDL88_ARATH1.9e-9867.23GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2... [more]
sp|Q6NMR9|GDL84_ARATH2.8e-6249.17GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2... [more]
sp|O80443|GDL46_ARATH1.3e-4339.21GDSL esterase/lipase At2g38180 OS=Arabidopsis thaliana OX=3702 GN=At2g38180 PE=2... [more]
sp|Q2TAA2|IAH1_HUMAN5.5e-4242.19Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Homo sapiens OX=9606 GN=IAH1 P... [more]
sp|Q9DB29|IAH1_MOUSE1.0e-4042.44Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Mus musculus OX=10090 GN=Iah1 ... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0KTH4|A0A0A0KTH4_CUCSA5.8e-12991.32Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G004790 PE=4 SV=1[more]
tr|A0A1S3BZF4|A0A1S3BZF4_CUCME1.9e-12790.50GDSL esterase/lipase At5g62930 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349471... [more]
tr|A0A1S3BYB4|A0A1S3BYB4_CUCME1.1e-11973.49GDSL esterase/lipase At5g62930 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349471... [more]
tr|A0A2P5CF24|A0A2P5CF24_9ROSA1.4e-10676.79Lipase OS=Trema orientalis OX=63057 GN=TorRG33x02_287230 PE=4 SV=1[more]
tr|A0A2P5AF70|A0A2P5AF70_PARAD2.4e-10676.37Lipase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_338450 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016788hydrolase activity, acting on ester bonds
Vocabulary: INTERPRO
TermDefinition
IPR001087GDSL
IPR036514SGNH_hydro_sf
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016788 hydrolase activity, acting on ester bonds

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg19723-RACarg19723-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3DG3DSA:3.40.50.1110coord: 2..236
e-value: 7.8E-71
score: 240.0
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 5..202
e-value: 6.8E-28
score: 97.9
NoneNo IPR availablePANTHERPTHR45192FAMILY NOT NAMEDcoord: 1..238
NoneNo IPR availableCDDcd01838Isoamyl_acetate_hydrolase_likecoord: 5..208
e-value: 7.64134E-96
score: 281.063
NoneNo IPR availableSUPERFAMILYSSF52266SGNH hydrolasecoord: 3..209