Carg18890 (gene) Silver-seed gourd

NameCarg18890
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionUbiquitin-conjugating enzyme, E2
LocationCucurbita_argyrosperma_scaffold_162 : 297554 .. 298462 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCGCTCTCTGCTATTCCCACGACGCCGAACACAAACAACCGCCCACCGCCCTCGAACCTGAGAGATCGCCACCGTTCAAACGATTTGATATTGTCACGGATTTCTCCGATCACCATTACGCTCACTCCAACTTGTTCCCCAACTGCATTGGATCGGATGTCTCGAAGAAGATCATGCGAGAGTGGAAGATTCTGGAGAGGAATCTTCCGGAATCGATTTTCGTCCGCGCTTACGAAGAGAGAATCGATCTTCTACGAGCCGTGATTGTCGGTCCGGCTGGAACTCCGTATCACGACGGATTGTTCTTCTTCGACCTGGCGTTCAGTTCCGATTATCCGATCTGTCCTCCGATTGTGTACTACCACTCCTACGGACTGCGATTGAACCCTAACCTGTACGAAGACGGCTATGTGTGTTTGAGTTTGATCAATACCTGGGACGGAAAAAAGGAAGAGCGCTGGAATCCGGCGATTTCAACAGTGCTTCAGATTCTGATATCGATTCAAGCGCTGGTGCTGAACGAGAGGCCGTACTTCAACGAACCGGAACTGGACGAGGCAGCGGAGGCGGAGATTTACTCGGACGCGTACAACAAGGAAGCGTACCTATTCTGCTGCAAGATGACGATGCGATTGATACAGAATCCGCCGAAGAATTTCGAGGAATTTGTAAGAGATTACTTCAGACAGCGAGGGAAGGGGATTCTGGCAGCTTGCAAGGCGTATTCGGAGGGGCGCGTTAGGGTCGGGAGGTTCAGTGGAAGTGAAACTGAAAATTGTAGCTGGAGCAGTAGCAGTAGTAACGGTGGTAACGGTACGCATGTTTCTGCCATTTTCAAGGCATTAATGGAGAAGACGCATCGGGATCTGCAAGCAGCTTTTGACGTGTCTAGCCAGGAATGA

mRNA sequence

ATGGCTTCCGCTCTCTGCTATTCCCACGACGCCGAACACAAACAACCGCCCACCGCCCTCGAACCTGAGAGATCGCCACCGTTCAAACGATTTGATATTGTCACGGATTTCTCCGATCACCATTACGCTCACTCCAACTTGTTCCCCAACTGCATTGGATCGGATGTCTCGAAGAAGATCATGCGAGAGTGGAAGATTCTGGAGAGGAATCTTCCGGAATCGATTTTCGTCCGCGCTTACGAAGAGAGAATCGATCTTCTACGAGCCGTGATTGTCGGTCCGGCTGGAACTCCGTATCACGACGGATTGTTCTTCTTCGACCTGGCGTTCAGTTCCGATTATCCGATCTGTCCTCCGATTGTGTACTACCACTCCTACGGACTGCGATTGAACCCTAACCTGTACGAAGACGGCTATGTGTGTTTGAGTTTGATCAATACCTGGGACGGAAAAAAGGAAGAGCGCTGGAATCCGGCGATTTCAACAGTGCTTCAGATTCTGATATCGATTCAAGCGCTGGTGCTGAACGAGAGGCCGTACTTCAACGAACCGGAACTGGACGAGGCAGCGGAGGCGGAGATTTACTCGGACGCGTACAACAAGGAAGCGTACCTATTCTGCTGCAAGATGACGATGCGATTGATACAGAATCCGCCGAAGAATTTCGAGGAATTTGTAAGAGATTACTTCAGACAGCGAGGGAAGGGGATTCTGGCAGCTTGCAAGGCGTATTCGGAGGGGCGCGTTAGGGTCGGGAGGTTCAGTGGAAGTGAAACTGAAAATTGTAGCTGGAGCAGTAGCAGTAGTAACGGTGGTAACGGTACGCATGTTTCTGCCATTTTCAAGGCATTAATGGAGAAGACGCATCGGGATCTGCAAGCAGCTTTTGACGTGTCTAGCCAGGAATGA

Coding sequence (CDS)

ATGGCTTCCGCTCTCTGCTATTCCCACGACGCCGAACACAAACAACCGCCCACCGCCCTCGAACCTGAGAGATCGCCACCGTTCAAACGATTTGATATTGTCACGGATTTCTCCGATCACCATTACGCTCACTCCAACTTGTTCCCCAACTGCATTGGATCGGATGTCTCGAAGAAGATCATGCGAGAGTGGAAGATTCTGGAGAGGAATCTTCCGGAATCGATTTTCGTCCGCGCTTACGAAGAGAGAATCGATCTTCTACGAGCCGTGATTGTCGGTCCGGCTGGAACTCCGTATCACGACGGATTGTTCTTCTTCGACCTGGCGTTCAGTTCCGATTATCCGATCTGTCCTCCGATTGTGTACTACCACTCCTACGGACTGCGATTGAACCCTAACCTGTACGAAGACGGCTATGTGTGTTTGAGTTTGATCAATACCTGGGACGGAAAAAAGGAAGAGCGCTGGAATCCGGCGATTTCAACAGTGCTTCAGATTCTGATATCGATTCAAGCGCTGGTGCTGAACGAGAGGCCGTACTTCAACGAACCGGAACTGGACGAGGCAGCGGAGGCGGAGATTTACTCGGACGCGTACAACAAGGAAGCGTACCTATTCTGCTGCAAGATGACGATGCGATTGATACAGAATCCGCCGAAGAATTTCGAGGAATTTGTAAGAGATTACTTCAGACAGCGAGGGAAGGGGATTCTGGCAGCTTGCAAGGCGTATTCGGAGGGGCGCGTTAGGGTCGGGAGGTTCAGTGGAAGTGAAACTGAAAATTGTAGCTGGAGCAGTAGCAGTAGTAACGGTGGTAACGGTACGCATGTTTCTGCCATTTTCAAGGCATTAATGGAGAAGACGCATCGGGATCTGCAAGCAGCTTTTGACGTGTCTAGCCAGGAATGA

Protein sequence

MASALCYSHDAEHKQPPTALEPERSPPFKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPYFNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAFDVSSQE
BLAST of Carg18890 vs. NCBI nr
Match: XP_022930350.1 (probable ubiquitin-conjugating enzyme E2 25 [Cucurbita moschata])

HSP 1 Score: 624.0 bits (1608), Expect = 2.6e-175
Identity = 300/302 (99.34%), Postives = 301/302 (99.67%), Query Frame = 0

Query: 1   MASALCYSHDAEHKQPPTALEPERSPPFKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKI 60
           MASALCYSHDAE KQPPTALEPERSPPFKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKI
Sbjct: 1   MASALCYSHDAEDKQPPTALEPERSPPFKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKI 60

Query: 61  MREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPI 120
           MREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPI
Sbjct: 61  MREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPI 120

Query: 121 VYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPY 180
           VYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPY
Sbjct: 121 VYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPY 180

Query: 181 FNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAA 240
           FNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAA
Sbjct: 181 FNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAA 240

Query: 241 CKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAFDVSS 300
           CKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAFD+SS
Sbjct: 241 CKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAFDLSS 300

Query: 301 QE 303
           QE
Sbjct: 301 QE 302

BLAST of Carg18890 vs. NCBI nr
Match: XP_022999956.1 (probable ubiquitin-conjugating enzyme E2 25 [Cucurbita maxima])

HSP 1 Score: 614.0 bits (1582), Expect = 2.7e-172
Identity = 297/302 (98.34%), Postives = 297/302 (98.34%), Query Frame = 0

Query: 1   MASALCYSHDAEHKQPPTALEPERSPPFKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKI 60
           MASALCYSH AE KQPPT  EPERSPPFKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKI
Sbjct: 1   MASALCYSHHAEDKQPPTPHEPERSPPFKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKI 60

Query: 61  MREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPI 120
           MREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPI PPI
Sbjct: 61  MREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPISPPI 120

Query: 121 VYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPY 180
           VYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPY
Sbjct: 121 VYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPY 180

Query: 181 FNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAA 240
           FNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAA
Sbjct: 181 FNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAA 240

Query: 241 CKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAFDVSS 300
           CKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAFDVSS
Sbjct: 241 CKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAFDVSS 300

Query: 301 QE 303
           QE
Sbjct: 301 QE 302

BLAST of Carg18890 vs. NCBI nr
Match: XP_023514441.1 (probable ubiquitin-conjugating enzyme E2 25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 524.6 bits (1350), Expect = 2.2e-145
Identity = 251/259 (96.91%), Postives = 251/259 (96.91%), Query Frame = 0

Query: 1   MASALCYSHDAEHKQPPTALEPERSPPFKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKI 60
           MASALCYSHDAE KQPPTALEPERS PFKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKI
Sbjct: 41  MASALCYSHDAEDKQPPTALEPERSTPFKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKI 100

Query: 61  MREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPI 120
           MREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPI PPI
Sbjct: 101 MREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPISPPI 160

Query: 121 VYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPY 180
           VYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPY
Sbjct: 161 VYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPY 220

Query: 181 FNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAA 240
           FNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGIL A
Sbjct: 221 FNEPELDEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILTA 280

Query: 241 CKAYSEGRVRVGRFSGSET 260
           CKAYSEGRVRVGRF    T
Sbjct: 281 CKAYSEGRVRVGRFXSVTT 299

BLAST of Carg18890 vs. NCBI nr
Match: XP_004148800.1 (PREDICTED: probable ubiquitin-conjugating enzyme E2 25 [Cucumis sativus] >KGN47459.1 hypothetical protein Csa_6G330470 [Cucumis sativus])

HSP 1 Score: 440.3 bits (1131), Expect = 5.4e-120
Identity = 224/300 (74.67%), Postives = 244/300 (81.33%), Query Frame = 0

Query: 10  DAEHKQPPT---ALEPER-----SPP---FKRFDIVTDFSDHHYAHSNLFPNCIGSDVSK 69
           D E K P T     EP R     SPP   FKRFDIVTD+SDHHYA SNLFPNCIGSDVSK
Sbjct: 14  DGEDKHPTTVDGGDEPNRSLSSPSPPSFSFKRFDIVTDYSDHHYADSNLFPNCIGSDVSK 73

Query: 70  KIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICP 129
           KIMREWK+LERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFFFDLAF+SDYP  P
Sbjct: 74  KIMREWKVLERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFFFDLAFTSDYPNTP 133

Query: 130 PIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNER 189
           P+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNP+ISTVLQILISIQ LVLNE+
Sbjct: 134 PMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQILISIQGLVLNEK 193

Query: 190 PYFNEPEL--DEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKG 249
           PYFNEPEL  DEA + EIYSDAYNKE YL CC+M MRL++NP KN+EEFVR+YF  +G+ 
Sbjct: 194 PYFNEPELEKDEADQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYEEFVRNYFGGKGER 253

Query: 250 ILAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAF 297
           ILAAC+AYS+GRVRVGR+  +E                  VSAIFK LMEK HRDL AAF
Sbjct: 254 ILAACEAYSDGRVRVGRY--NEXXXXXXXXXXXXNCKRGDVSAIFKTLMEKMHRDLHAAF 311

BLAST of Carg18890 vs. NCBI nr
Match: XP_008458344.1 (PREDICTED: putative ubiquitin-conjugating enzyme E2 39 [Cucumis melo])

HSP 1 Score: 434.9 bits (1117), Expect = 2.3e-118
Identity = 222/306 (72.55%), Postives = 244/306 (79.74%), Query Frame = 0

Query: 10  DAEHKQPPT---ALEPERSP----------PFKRFDIVTDFSDHHYAHSNLFPNCIGSDV 69
           D E K P T     EP RS            FKRFDIV+D+SDHHYA SNLFPNCIGSDV
Sbjct: 13  DGEDKHPTTVDGGDEPNRSLSSXXXXXXSFSFKRFDIVSDYSDHHYADSNLFPNCIGSDV 72

Query: 70  SKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPI 129
           SKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFFFDLAF+SDYPI
Sbjct: 73  SKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFFFDLAFTSDYPI 132

Query: 130 CPPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLN 189
            PP+VY HSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNP+ISTVLQILISIQALVLN
Sbjct: 133 TPPMVYCHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQILISIQALVLN 192

Query: 190 ERPYFNEPELDEAA---EAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQR 249
           E+PYFNEPEL+E     + EIYSDAYNKE YL CC+M MRL++NP KN+EEFVR+YF ++
Sbjct: 193 EKPYFNEPELEEEEADDQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYEEFVRNYFGEK 252

Query: 250 GKGILAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGT---HVSAIFKALMEKTHR 297
           G+ ILAAC+AYS+GRVRVGR++                        VSAIFKALMEKTHR
Sbjct: 253 GERILAACEAYSDGRVRVGRYNXXXXXXXXXXXXXXXXXETCKRGDVSAIFKALMEKTHR 312

BLAST of Carg18890 vs. TAIR10
Match: AT2G33770.1 (phosphate 2)

HSP 1 Score: 261.2 bits (666), Expect = 8.2e-70
Identity = 140/285 (49.12%), Postives = 182/285 (63.86%), Query Frame = 0

Query: 18  TALEPERSP-PFKRFDIVTDFSDHHYAHSN--LFPNCIGSDVSKKIMREWKILERNLPES 77
           T LE  R+   F++FD+V D SDHH+  S+  L  + +     KK+ +EW  LE NLP +
Sbjct: 622 TLLENSRNQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNT 681

Query: 78  IFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPIVYYHSYGLRLNPNL 137
           I+VR  EER+DLLRA +VG  GTPYHDGLFFFD+     YP  PP+V+YHS G+RLNPNL
Sbjct: 682 IYVRVCEERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNL 741

Query: 138 YEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPYFNEPELDE---AAE 197
           YE G VCLSL+NTW G   E WN   S++LQ+L+S QALVLNE+PYFNE   D+    AE
Sbjct: 742 YESGRVCLSLLNTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAE 801

Query: 198 AEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAACKAYSEGRVRV 257
            E  S +YN+ A+L  CK  + +++ PPK+FE  V+D+F  R + +LAACKAY EG V V
Sbjct: 802 GEKNSVSYNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEG-VPV 861

Query: 258 GRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAF 297
           G             SS++  GN T  S  FK ++ K +  L  AF
Sbjct: 862 G-------------SSANLQGNSTTNSTGFKIMLSKLYPKLLEAF 892

BLAST of Carg18890 vs. TAIR10
Match: AT3G15355.1 (ubiquitin-conjugating enzyme 25)

HSP 1 Score: 256.9 bits (655), Expect = 1.5e-68
Identity = 127/256 (49.61%), Postives = 161/256 (62.89%), Query Frame = 0

Query: 8   SHDAEHKQPPTALEPERSPP-------------FKRFDIVTDFSDHHYAHSNLFPNCIGS 67
           S+ A  K+ P  L P+ S               FKRFDIV DFSDHHYA           
Sbjct: 272 SNAARFKEEPPILPPDDSRVKRNMEDYLGLYLFFKRFDIVEDFSDHHYASKGTTSKQHSK 331

Query: 68  DVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDY 127
           D +K+I  EW+ILE++LPE IFVRAYE R+DLLRAVI+G  GTPYHDGLFFFD+ F   Y
Sbjct: 332 DWAKRIQDEWRILEKDLPEMIFVRAYESRMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTY 391

Query: 128 PICPPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALV 187
           P  PPIV+YHS GLR+NPNLY  G VCLSL+ TW G + E+W P  ST+LQ+L+SIQ L+
Sbjct: 392 PSTPPIVHYHSGGLRINPNLYNCGKVCLSLLGTWSGNQREKWIPNTSTMLQVLVSIQGLI 451

Query: 188 LNERPYFNEPELDE---AAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFR 247
           LN++PYFNEP  +    +A  E  S AY++  ++   K  +  ++ PPK FE+F   +F 
Sbjct: 452 LNQKPYFNEPGYERSAGSAHGESTSKAYSENTFILSLKTMVYTMRRPPKYFEDFAYGHFF 511

BLAST of Carg18890 vs. TAIR10
Match: AT2G16920.1 (ubiquitin-conjugating enzyme 23)

HSP 1 Score: 251.1 bits (640), Expect = 8.4e-67
Identity = 140/287 (48.78%), Postives = 179/287 (62.37%), Query Frame = 0

Query: 12   EHKQPPTALEPERSPPFKRFDIVTDFSDHHYAHSNLFPNCIGSDVS--------KKIMRE 71
            E +  P   E + S  F+RFDI  D  DHH+         +G D          KK+ ++
Sbjct: 809  EDEDKPVPSEGD-SCSFRRFDISQDPLDHHF---------LGVDGQKTKERQWFKKVDQD 868

Query: 72   WKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPIVYY 131
            WKIL+ NLP+ IFVRAYE+R+DLLRAVIVG  GTPY DGLFFFD    SDYP  PP  YY
Sbjct: 869  WKILQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYY 928

Query: 132  HSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPYFNE 191
            HS G RLNPNLYE+G VCLSL+NTW G+  E W+P  S++LQ+L+S+Q LVLN +PYFNE
Sbjct: 929  HSGGWRLNPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNE 988

Query: 192  PELDE---AAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAA 251
               D+    AE E  S  YN+  +L  CK  M L++ PPK+FEE ++D+FR+RG  IL A
Sbjct: 989  AGYDKQVGTAEGEKNSLGYNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKA 1048

Query: 252  CKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEK 288
            C AY +G +      GS T++ S     S+  N T V   FK ++ K
Sbjct: 1049 CDAYMKGYL-----IGSLTKDASVIDERSS-ANSTSVG--FKLMLAK 1077

BLAST of Carg18890 vs. TAIR10
Match: AT1G53025.1 (Ubiquitin-conjugating enzyme family protein)

HSP 1 Score: 245.7 bits (626), Expect = 3.5e-65
Identity = 120/231 (51.95%), Postives = 153/231 (66.23%), Query Frame = 0

Query: 20  LEPERSPPFKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKIMREWKILERNLPESIFVRA 79
           L+ E    FKRFD V DFSDHHYA           +  KK+  +WKILE +LPE+I VRA
Sbjct: 236 LKEEFLRDFKRFDTVEDFSDHHYASKGKSSKQHSKNWVKKVQADWKILENDLPEAISVRA 295

Query: 80  YEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPIVYYHSYGLRLNPNLYEDGY 139
            E R+DLLRAVI+G  GTPYHDGLFFFD+ F   YP  PP V+YHS GLR+NPNLY  G 
Sbjct: 296 CESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCGK 355

Query: 140 VCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPYFNEPELDEA---AEAEIYS 199
           VCLSL+ TW G   E+W P  ST+LQ+L+SIQAL+LNE+PYFNEP   ++   A  E  S
Sbjct: 356 VCLSLLGTWAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESKS 415

Query: 200 DAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAACKAYSEG 248
             Y++  +L   K  +  I+ PP++FEE+V++++  R   I+ AC AY  G
Sbjct: 416 KVYSENVFLLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAG 466

BLAST of Carg18890 vs. TAIR10
Match: AT1G53023.1 (Ubiquitin-conjugating enzyme family protein)

HSP 1 Score: 242.7 bits (618), Expect = 3.0e-64
Identity = 128/240 (53.33%), Postives = 159/240 (66.25%), Query Frame = 0

Query: 20  LEPERSPPFKRFDIVTDFSDHHYAHS---------NLFPNCIGSDVSKKIMREWKILERN 79
           L+ E    FKRFD V DFSDHHYA           +L       +  K I +EWKIL++N
Sbjct: 13  LKEEFLRDFKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILDKN 72

Query: 80  LPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPIVYYHSYGLRL 139
           LPE+IFVRA E RIDLLRAVI+G  GTPYHDGLFFFD+ F   YP  PP V+YHS GLR+
Sbjct: 73  LPETIFVRACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHSGGLRI 132

Query: 140 NPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPYFNEPELDEAA 199
           NPNLY+ G VCLSLI+TW GKK E+W P  ST+LQ+L+SIQAL+LNE+PY+NEP  +++ 
Sbjct: 133 NPNLYKCGKVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEKSM 192

Query: 200 E---AEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAACKAYSEG 248
                E YS  Y++  ++F  K TM        +FEEFVR +F  R   I+ AC AY +G
Sbjct: 193 GTPLGESYSKDYSENVFVFSLK-TM--------HFEEFVRSHFFVRSHDIVKACNAYKDG 243

BLAST of Carg18890 vs. Swiss-Prot
Match: sp|Q8VY10|UBC24_ARATH (Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=UBC24 PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.5e-68
Identity = 140/285 (49.12%), Postives = 182/285 (63.86%), Query Frame = 0

Query: 18  TALEPERSP-PFKRFDIVTDFSDHHYAHSN--LFPNCIGSDVSKKIMREWKILERNLPES 77
           T LE  R+   F++FD+V D SDHH+  S+  L  + +     KK+ +EW  LE NLP +
Sbjct: 622 TLLENSRNQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNT 681

Query: 78  IFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPIVYYHSYGLRLNPNL 137
           I+VR  EER+DLLRA +VG  GTPYHDGLFFFD+     YP  PP+V+YHS G+RLNPNL
Sbjct: 682 IYVRVCEERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNL 741

Query: 138 YEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPYFNEPELDE---AAE 197
           YE G VCLSL+NTW G   E WN   S++LQ+L+S QALVLNE+PYFNE   D+    AE
Sbjct: 742 YESGRVCLSLLNTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAE 801

Query: 198 AEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAACKAYSEGRVRV 257
            E  S +YN+ A+L  CK  + +++ PPK+FE  V+D+F  R + +LAACKAY EG V V
Sbjct: 802 GEKNSVSYNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEG-VPV 861

Query: 258 GRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAF 297
           G             SS++  GN T  S  FK ++ K +  L  AF
Sbjct: 862 G-------------SSANLQGNSTTNSTGFKIMLSKLYPKLLEAF 892

BLAST of Carg18890 vs. Swiss-Prot
Match: sp|Q9LUQ5|UBC25_ARATH (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=UBC25 PE=2 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.8e-67
Identity = 127/256 (49.61%), Postives = 161/256 (62.89%), Query Frame = 0

Query: 8   SHDAEHKQPPTALEPERSPP-------------FKRFDIVTDFSDHHYAHSNLFPNCIGS 67
           S+ A  K+ P  L P+ S               FKRFDIV DFSDHHYA           
Sbjct: 272 SNAARFKEEPPILPPDDSRVKRNMEDYLGLYLFFKRFDIVEDFSDHHYASKGTTSKQHSK 331

Query: 68  DVSKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDY 127
           D +K+I  EW+ILE++LPE IFVRAYE R+DLLRAVI+G  GTPYHDGLFFFD+ F   Y
Sbjct: 332 DWAKRIQDEWRILEKDLPEMIFVRAYESRMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTY 391

Query: 128 PICPPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALV 187
           P  PPIV+YHS GLR+NPNLY  G VCLSL+ TW G + E+W P  ST+LQ+L+SIQ L+
Sbjct: 392 PSTPPIVHYHSGGLRINPNLYNCGKVCLSLLGTWSGNQREKWIPNTSTMLQVLVSIQGLI 451

Query: 188 LNERPYFNEPELDE---AAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFR 247
           LN++PYFNEP  +    +A  E  S AY++  ++   K  +  ++ PPK FE+F   +F 
Sbjct: 452 LNQKPYFNEPGYERSAGSAHGESTSKAYSENTFILSLKTMVYTMRRPPKYFEDFAYGHFF 511

BLAST of Carg18890 vs. Swiss-Prot
Match: sp|Q9ZVX1|UBC23_ARATH (Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=UBC23 PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.5e-65
Identity = 140/287 (48.78%), Postives = 179/287 (62.37%), Query Frame = 0

Query: 12   EHKQPPTALEPERSPPFKRFDIVTDFSDHHYAHSNLFPNCIGSDVS--------KKIMRE 71
            E +  P   E + S  F+RFDI  D  DHH+         +G D          KK+ ++
Sbjct: 809  EDEDKPVPSEGD-SCSFRRFDISQDPLDHHF---------LGVDGQKTKERQWFKKVDQD 868

Query: 72   WKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPIVYY 131
            WKIL+ NLP+ IFVRAYE+R+DLLRAVIVG  GTPY DGLFFFD    SDYP  PP  YY
Sbjct: 869  WKILQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTPYQDGLFFFDFHLPSDYPSVPPSAYY 928

Query: 132  HSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPYFNE 191
            HS G RLNPNLYE+G VCLSL+NTW G+  E W+P  S++LQ+L+S+Q LVLN +PYFNE
Sbjct: 929  HSGGWRLNPNLYEEGKVCLSLLNTWTGRGNEVWDPKSSSILQVLVSLQGLVLNSKPYFNE 988

Query: 192  PELDE---AAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAA 251
               D+    AE E  S  YN+  +L  CK  M L++ PPK+FEE ++D+FR+RG  IL A
Sbjct: 989  AGYDKQVGTAEGEKNSLGYNENTFLLNCKTMMYLMRKPPKDFEELIKDHFRKRGYYILKA 1048

Query: 252  CKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEK 288
            C AY +G +      GS T++ S     S+  N T V   FK ++ K
Sbjct: 1049 CDAYMKGYL-----IGSLTKDASVIDERSS-ANSTSVG--FKLMLAK 1077

BLAST of Carg18890 vs. Swiss-Prot
Match: sp|Q8GY87|UBC26_ARATH (Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana OX=3702 GN=UBC26 PE=2 SV=2)

HSP 1 Score: 245.7 bits (626), Expect = 6.4e-64
Identity = 120/231 (51.95%), Postives = 153/231 (66.23%), Query Frame = 0

Query: 20  LEPERSPPFKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKIMREWKILERNLPESIFVRA 79
           L+ E    FKRFD V DFSDHHYA           +  KK+  +WKILE +LPE+I VRA
Sbjct: 236 LKEEFLRDFKRFDTVEDFSDHHYASKGKSSKQHSKNWVKKVQADWKILENDLPEAISVRA 295

Query: 80  YEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPIVYYHSYGLRLNPNLYEDGY 139
            E R+DLLRAVI+G  GTPYHDGLFFFD+ F   YP  PP V+YHS GLR+NPNLY  G 
Sbjct: 296 CESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNCGK 355

Query: 140 VCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPYFNEPELDEA---AEAEIYS 199
           VCLSL+ TW G   E+W P  ST+LQ+L+SIQAL+LNE+PYFNEP   ++   A  E  S
Sbjct: 356 VCLSLLGTWAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGESKS 415

Query: 200 DAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAACKAYSEG 248
             Y++  +L   K  +  I+ PP++FEE+V++++  R   I+ AC AY  G
Sbjct: 416 KVYSENVFLLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAG 466

BLAST of Carg18890 vs. Swiss-Prot
Match: sp|Q9C918|UBC38_ARATH (Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=UBC38 PE=3 SV=2)

HSP 1 Score: 244.2 bits (622), Expect = 1.9e-63
Identity = 118/223 (52.91%), Postives = 147/223 (65.92%), Query Frame = 0

Query: 28  FKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLL 87
           FKRFD V DFSDHHY            +  KK+  EWKIL +NLPE+IFVRA E R+DLL
Sbjct: 27  FKRFDSVEDFSDHHYVSKGKASKQHSKNWVKKVQDEWKILNQNLPETIFVRACESRMDLL 86

Query: 88  RAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPIVYYHSYGLRLNPNLYEDGYVCLSLINT 147
           RAVI+G  GTPYHDGLFFFD+ F   YP  PP VYYHS GLR+NPNLY  G VCLSL+ T
Sbjct: 87  RAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVYYHSGGLRINPNLYNCGKVCLSLLGT 146

Query: 148 WDGKKEERWNPAISTVLQILISIQALVLNERPYFNEPE---LDEAAEAEIYSDAYNKEAY 207
           W G   + W P  ST+LQ+L+SIQALVLNE+PYFNEP    +      E  S  Y++  +
Sbjct: 147 WHGNARQSWLPKESTMLQVLVSIQALVLNEQPYFNEPGYGLIKGTWLGESKSKVYSENVF 206

Query: 208 LFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAACKAYSEG 248
           L   K  +  ++ PP++FEE+V++++  R   I+ AC AY  G
Sbjct: 207 LLSLKTMVYSMRKPPQHFEEYVQNHYFVRSHDIVKACNAYKAG 249

BLAST of Carg18890 vs. TrEMBL
Match: tr|A0A0A0KF71|A0A0A0KF71_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G330470 PE=4 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 3.6e-120
Identity = 224/300 (74.67%), Postives = 244/300 (81.33%), Query Frame = 0

Query: 10  DAEHKQPPT---ALEPER-----SPP---FKRFDIVTDFSDHHYAHSNLFPNCIGSDVSK 69
           D E K P T     EP R     SPP   FKRFDIVTD+SDHHYA SNLFPNCIGSDVSK
Sbjct: 14  DGEDKHPTTVDGGDEPNRSLSSPSPPSFSFKRFDIVTDYSDHHYADSNLFPNCIGSDVSK 73

Query: 70  KIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICP 129
           KIMREWK+LERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFFFDLAF+SDYP  P
Sbjct: 74  KIMREWKVLERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFFFDLAFTSDYPNTP 133

Query: 130 PIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNER 189
           P+VYYHSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNP+ISTVLQILISIQ LVLNE+
Sbjct: 134 PMVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQILISIQGLVLNEK 193

Query: 190 PYFNEPEL--DEAAEAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKG 249
           PYFNEPEL  DEA + EIYSDAYNKE YL CC+M MRL++NP KN+EEFVR+YF  +G+ 
Sbjct: 194 PYFNEPELEKDEADQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYEEFVRNYFGGKGER 253

Query: 250 ILAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAF 297
           ILAAC+AYS+GRVRVGR+  +E                  VSAIFK LMEK HRDL AAF
Sbjct: 254 ILAACEAYSDGRVRVGRY--NEXXXXXXXXXXXXNCKRGDVSAIFKTLMEKMHRDLHAAF 311

BLAST of Carg18890 vs. TrEMBL
Match: tr|A0A1S3C8W9|A0A1S3C8W9_CUCME (putative ubiquitin-conjugating enzyme E2 39 OS=Cucumis melo OX=3656 GN=LOC103497784 PE=4 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.5e-118
Identity = 222/306 (72.55%), Postives = 244/306 (79.74%), Query Frame = 0

Query: 10  DAEHKQPPT---ALEPERSP----------PFKRFDIVTDFSDHHYAHSNLFPNCIGSDV 69
           D E K P T     EP RS            FKRFDIV+D+SDHHYA SNLFPNCIGSDV
Sbjct: 13  DGEDKHPTTVDGGDEPNRSLSSXXXXXXSFSFKRFDIVSDYSDHHYADSNLFPNCIGSDV 72

Query: 70  SKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPI 129
           SKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGP+GTPYHDGLFFFDLAF+SDYPI
Sbjct: 73  SKKIMREWKILERNLPESIFVRAYEERIDLLRAVIVGPSGTPYHDGLFFFDLAFTSDYPI 132

Query: 130 CPPIVYYHSYGLRLNPNLYEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLN 189
            PP+VY HSYGLRLNPNLYEDGYVCLSLINTWDGK+EERWNP+ISTVLQILISIQALVLN
Sbjct: 133 TPPMVYCHSYGLRLNPNLYEDGYVCLSLINTWDGKEEERWNPSISTVLQILISIQALVLN 192

Query: 190 ERPYFNEPELDEAA---EAEIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQR 249
           E+PYFNEPEL+E     + EIYSDAYNKE YL CC+M MRL++NP KN+EEFVR+YF ++
Sbjct: 193 EKPYFNEPELEEEEADDQGEIYSDAYNKEVYLLCCEMMMRLMKNPVKNYEEFVRNYFGEK 252

Query: 250 GKGILAACKAYSEGRVRVGRFSGSETENCSWSSSSSNGGNGT---HVSAIFKALMEKTHR 297
           G+ ILAAC+AYS+GRVRVGR++                        VSAIFKALMEKTHR
Sbjct: 253 GERILAACEAYSDGRVRVGRYNXXXXXXXXXXXXXXXXXETCKRGDVSAIFKALMEKTHR 312

BLAST of Carg18890 vs. TrEMBL
Match: tr|A0A2P2ILQ0|A0A2P2ILQ0_RHIMU (Uncharacterized protein MANES_15G000600 OS=Rhizophora mucronata OX=61149 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 2.7e-83
Identity = 160/285 (56.14%), Postives = 199/285 (69.82%), Query Frame = 0

Query: 23  ERSPPFKRFDIVTDFSDHHYAHSNL--------FPNCIGSDVSKKIMREWKILERNLPES 82
           +++  FK+FDIV D SDH Y +SN+        F N  GS+V KKIM+EW+ILE NLPES
Sbjct: 26  QKTSRFKQFDIVADHSDHFYTYSNMSAGKKGDCFKNA-GSNVYKKIMQEWRILENNLPES 85

Query: 83  IFVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPIVYYHSYGLRLNPNL 142
           IFVR YE RIDLLRAVI+G  GTPYHDGLFFFD AF SDYP  PP+V+YHS+GL LNPNL
Sbjct: 86  IFVRVYEGRIDLLRAVIIGAQGTPYHDGLFFFDFAFPSDYPNNPPLVHYHSHGLELNPNL 145

Query: 143 YEDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPYFNEPELDE--AAEA 202
           Y++GYVCLSL+NTW  ++ E WNP  STVLQ L+S+QALVLNE+PY+NEP  +E  + + 
Sbjct: 146 YDNGYVCLSLLNTWSAEETEMWNPTGSTVLQALVSLQALVLNEKPYYNEPGYEEPSSGDW 205

Query: 203 EIYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAACKAYSEGRVRVG 262
           ++ S  YN E +   CK  M LI NPPKNFE FV+D+F +R   ILAAC+AY EGR RVG
Sbjct: 206 DLDSAEYNGEVFGLSCKTMMFLIDNPPKNFEAFVKDHFCERANVILAACRAYIEGRARVG 265

Query: 263 RFSGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAFD 298
            + GS +  C+             VS  FK  ME  + +L+ AF+
Sbjct: 266 YYDGSPSSPCT-----------VDVSREFKGTMEALYPNLEVAFN 298

BLAST of Carg18890 vs. TrEMBL
Match: tr|A0A2P6QZ65|A0A2P6QZ65_ROSCH (Putative aminoacyltransferase, E1 ubiquitin-activating enzyme OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0425551 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.4e-79
Identity = 158/284 (55.63%), Postives = 193/284 (67.96%), Query Frame = 0

Query: 16  PPTALEPERSPPFKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKIMREWKILERNLPESI 75
           P T  +   + PF RFD+V+D SDH+Y+ S       G  V K IM+EW+ILE NLP+SI
Sbjct: 44  PITMTQNSDATPFTRFDVVSDHSDHYYSASGK-KQKYGGSVHKLIMKEWRILENNLPDSI 103

Query: 76  FVRAYEERIDLLRAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPIVYYHSYGLRLNPNLY 135
           +VR YE RIDLLRAVIVG AGTPYHD LFFFD+ F SDYP  PP V+Y SYGLR+NPNLY
Sbjct: 104 YVRVYETRIDLLRAVIVGAAGTPYHDALFFFDINFPSDYPTRPPNVHYRSYGLRVNPNLY 163

Query: 136 EDGYVCLSLINTWDGKKEERWNPAISTVLQILISIQALVLNERPYFNEPELDE--AAEAE 195
             G+VCLSL+NTW GKK E+WNPA STVLQ+L+SIQALVLNE+PYFNEP       +  E
Sbjct: 164 ASGFVCLSLLNTWTGKKREKWNPAQSTVLQVLVSIQALVLNEKPYFNEPGTGSPGGSAGE 223

Query: 196 IYSDAYNKEAYLFCCKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAACKAYSEGRVRVGR 255
             S AYN++A++  CK T+  ++NPPKNFE F  +++R+RG  I  AC AY  GRV VG 
Sbjct: 224 KRSRAYNEDAFVLTCKTTLFSLRNPPKNFEAFTAEHYRERGSRIARACGAYVSGRVGVGY 283

Query: 256 F-SGSETENCSWSSSSSNGGNGTHVSAIFKALMEKTHRDLQAAF 297
           +  G E E CS S S         VS  F  LM++ +  L  AF
Sbjct: 284 YRDGVEFEPCSSSKSKV-------VSKKFVGLMQELYPQLVQAF 319

BLAST of Carg18890 vs. TrEMBL
Match: tr|A0A1U8AKS5|A0A1U8AKS5_NELNU (putative ubiquitin-conjugating enzyme E2 38 OS=Nelumbo nucifera OX=4432 GN=LOC104605032 PE=4 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.2e-78
Identity = 144/244 (59.02%), Postives = 184/244 (75.41%), Query Frame = 0

Query: 28  FKRFDIVTDFSDHHYAHSNLFPNCIGSDVSKKIMREWKILERNLPESIFVRAYEERIDLL 87
           FK+FD+V+DF+DH+Y   ++  + I S+  KKIMREWKILE NLPESIFVRAYE R+DLL
Sbjct: 7   FKQFDVVSDFTDHYYYGPSV--SIISSNARKKIMREWKILENNLPESIFVRAYEGRMDLL 66

Query: 88  RAVIVGPAGTPYHDGLFFFDLAFSSDYPICPPIVYYHSYGLRLNPNLYEDGYVCLSLINT 147
           RAVI+G AGTPYHDGLFFFD+ F SDYP  PP V+YHS+GLR+NPNLY+ GYVCLSL+NT
Sbjct: 67  RAVIIGAAGTPYHDGLFFFDILFPSDYPARPPQVHYHSHGLRINPNLYKSGYVCLSLLNT 126

Query: 148 WDGKKEERWNPAISTVLQILISIQALVLNERPYFNEPELDEAAEAEIYSDAYNKEAYLFC 207
           W G K E+WN + STVLQ+L+SIQALVLNE P+FNEP     + +   S  YN+ A++  
Sbjct: 127 WHGHKNEKWNSSASTVLQVLVSIQALVLNENPFFNEPGAIHWSSSTKASSDYNETAFVLS 186

Query: 208 CKMTMRLIQNPPKNFEEFVRDYFRQRGKGILAACKAYSEGRVRVGRF----SGSETENCS 267
           C+  + L+Q PPKNFE F+ D+FR+RG+ IL AC+AY +G  +VG      SG +T   S
Sbjct: 187 CRTMLYLLQKPPKNFERFLVDHFRKRGEAILTACRAYRDGFAKVGGVVHVQSGGDTATPS 246

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022930350.12.6e-17599.34probable ubiquitin-conjugating enzyme E2 25 [Cucurbita moschata][more]
XP_022999956.12.7e-17298.34probable ubiquitin-conjugating enzyme E2 25 [Cucurbita maxima][more]
XP_023514441.12.2e-14596.91probable ubiquitin-conjugating enzyme E2 25 [Cucurbita pepo subsp. pepo][more]
XP_004148800.15.4e-12074.67PREDICTED: probable ubiquitin-conjugating enzyme E2 25 [Cucumis sativus] >KGN474... [more]
XP_008458344.12.3e-11872.55PREDICTED: putative ubiquitin-conjugating enzyme E2 39 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT2G33770.18.2e-7049.12phosphate 2[more]
AT3G15355.11.5e-6849.61ubiquitin-conjugating enzyme 25[more]
AT2G16920.18.4e-6748.78ubiquitin-conjugating enzyme 23[more]
AT1G53025.13.5e-6551.95Ubiquitin-conjugating enzyme family protein[more]
AT1G53023.13.0e-6453.33Ubiquitin-conjugating enzyme family protein[more]
Match NameE-valueIdentityDescription
sp|Q8VY10|UBC24_ARATH1.5e-6849.12Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
sp|Q9LUQ5|UBC25_ARATH2.8e-6749.61Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
sp|Q9ZVX1|UBC23_ARATH1.5e-6548.78Probable ubiquitin-conjugating enzyme E2 23 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
sp|Q8GY87|UBC26_ARATH6.4e-6451.95Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
sp|Q9C918|UBC38_ARATH1.9e-6352.91Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana OX=3702 GN=U... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0KF71|A0A0A0KF71_CUCSA3.6e-12074.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G330470 PE=4 SV=1[more]
tr|A0A1S3C8W9|A0A1S3C8W9_CUCME1.5e-11872.55putative ubiquitin-conjugating enzyme E2 39 OS=Cucumis melo OX=3656 GN=LOC103497... [more]
tr|A0A2P2ILQ0|A0A2P2ILQ0_RHIMU2.7e-8356.14Uncharacterized protein MANES_15G000600 OS=Rhizophora mucronata OX=61149 PE=4 SV... [more]
tr|A0A2P6QZ65|A0A2P6QZ65_ROSCH1.4e-7955.63Putative aminoacyltransferase, E1 ubiquitin-activating enzyme OS=Rosa chinensis ... [more]
tr|A0A1U8AKS5|A0A1U8AKS5_NELNU1.2e-7859.02putative ubiquitin-conjugating enzyme E2 38 OS=Nelumbo nucifera OX=4432 GN=LOC10... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR016135UBQ-conjugating_enzyme/RWD
IPR000608UBQ-conjugat_E2
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg18890-RACarg18890-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 58..212
e-value: 1.7E-23
score: 94.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR44273:SF4UBIQUITIN-CONJUGATING ENZYME E2 39-RELATEDcoord: 21..297
NoneNo IPR availablePANTHERPTHR44273FAMILY NOT NAMEDcoord: 21..297
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 60..197
e-value: 7.4E-24
score: 83.9
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBIQUITIN_CONJUGAT_2coord: 58..191
score: 22.353
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 57..209
e-value: 1.20591E-37
score: 131.17
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3DG3DSA:3.10.110.10coord: 22..294
e-value: 1.1E-74
score: 253.3
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILYSSF54495UBC-likecoord: 51..219

The following gene(s) are paralogous to this gene:

None