Carg18385 (gene) Silver-seed gourd

NameCarg18385
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionEukaryotic translation initiation factor 2 subunit alpha
LocationCucurbita_argyrosperma_scaffold_106 : 449867 .. 454356 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATCTTCTCCATCAAACTCCCTCTCTCTCTCTCTCTCTAACAATTAGCCCTAAATTCCCCCAAAAGAAAACCCCATTTCGTCTCTTAAACCCTTCTTCACAGAATCCTTCAATTCTCTGAAATACTCTCTACCATGGCTTCCCATTCCCCTAATCTCGAGTGTCGGATGTACGAAGCCAAGTACCCCGAGGTCGACATGGCCGTCATGATCCAGGTCAAGAACATTGCTGACATGGGCGCTTACGTCTCTCTTCTTGAGTACAACAACATCGAGGGTATGATTCTCTTCTCCGAGCTCTCTCGCCGTCGTATTCGTAGTGTCAGCAGCTTAATTAGGGTCGGTCGAATCGAGCCCGTCATGGTCCTCAGGGTCGATAAGGAGAAGGGGTATATTGATTTGAGTAAGAGGAGGGTTTCTGAGGAGGATATTCAGGCATGTGAGGAGAGGTATAACAAGAGCAAGCTTGTTCACTCTATAATGCGCCATGTTGCTGAGACTGAAAATATTGATTTGGAGGTAGTTTCTCGTTTTCTGGCTTTCTTTTTGCTGGCTCTTTTTCTCATTATTGGCTGTGTTGCTTTGATGGCTGTTTTAGGAGTTCGTTGAAGTTTTAGTTTCTTGGGTGATGTTTGAATTTGTTTTCGTGTATTCTTTTTGTTTGAGTTAATTAATATTATATGTGTAAGCACTCTGAATTGACCTAGTGATTATTGGAGTAGGGAAACGGAAGGTGAAGTTCTCCTAGGCTGTGGTTCAAAACTATGGTGGCCAGTGGTCGCCTTTGACTTTAAAAATTCTGCGTGTTTCTTGACAACAACCAAATATAATAGTTTCTTCTGGTTAATTAGTGGGGCGTGCCTAAGTTGACTACTCGATACATAGATATGTAGAAAAAAGTTCCTTAGTTTACGAAGAGAGTTGGATTTGGCTCCTTTGGATTAACAACATTGGATTGAAGGGGCAGGTATTTAACAATTGGTGAAGGGCTTAAAACTAGCCAAGATTGTGTTATTCAATTTTTCTGCGATGAATTCAAGCACACGGTGATGAGTGAAGGGTGAGCAAATGCATATTCCTTGTGCAATGGGGCACACCTTGATCACCAAGTTCATGGCAAATGGCAATCACCATTTTCTTGGGTTTAAATTCAGGACACTCATCACCAGTTTCCTAAGGTGGCTTCGAGGCTTTGTTTTAGAAGGGGAGGCATAAGACTTGAAGCTCTATGTGGTGTATGCATTGATGTAAATACAAATAATAATAATAATGATAAAAATTGTAAAAAGGTAAGCCATGCACTAGTAAACACATCCATCTTGTTGTAAAGGTCATTCTCATCATCATTATTATCATTGATAGGATTAACCTTTTCTTTAATTTTTATTTTTTGTACTTTATTAAGCATTAGTTGTAAAGTTAGTAAAAGGCCAAATAACCACAGCTCTTGTTCATATTTGGACTTTTATGTCTTGAAGAATATGCATTAAGTTTATATAAAAATTCTTTGAGGCACATGCCACAATGACTTTGTTTGAGTGCATTCTCATGTAGGTGTTAGTCTTCGAACGCCTTGAGATTTAAATTTTGAGCCCTATCTCAAGTTGTAAGCCTTGAAAGCCTTTAAATCCAAAAATAAAAATAAAAATGCAACTAGCAAAAGGTGTTGTACTTTCCTTCTTAATTTAACAAAATTCTCCTTGCTTTGTATGTTAGTGTTTCTTCTTTTGATTCCCTCAAAGAATTCGTTTTCATCCCTTAAACACAAAAACCAATAAAGTTACATGGTTTTAAGTCTCTCCTTGCATCCATCTTTCCAATTCTCTCTTTTGATTGAATAGAATAAGTTTAAGAAACTATACATTGTGATTTATTAAATATTGATATATTAAGTTCAAGAAACTATAGATTGTGATGTATCGAATATTGATATATTAAGTATTGATATTAATATTTCTTGATTTATGGCTTAATTCTCTTTGTGATTCATTGATCTATATTAACTTCTATTTATGTAAACTAAATCACTTAGCTTTGCTGCGTGATGCATATGTACTCTTGTCTCCTAATGATCAAGTAGCTTGACATTTTGGTCTGCACTTTGTACATTGCGCCCTGGAAACCTCGAGCACCTTTTATTGTTGTCTCCTATGTATTGTATCTTTTTGAGTTATTTGATCCAATCCCCCGTCAAGGAACCTAGAGTTTGATGAAACAGTGAAAGGAAGTACCTCGTTAATTGTGAAATGGCCTTTAACCCTTGGTTTGTTTTAGCTATATTATGTATTAGTTTGACATTAATTGCTAATAATGATCTATTCTAGAAAAAAAATGGCTTGTGCGTGTTCCAGAATCTGCCCTCCCCCACTCTCATGCACACAAGCACAGGAAAAAAAAAAAAAAAAGATGCAAAATGCTTCTTTATGTGAAGATCTTCCAAGGCCTTAATATTCTGTTGTATTAAAGCAAGGTTGTCAAAATGCTAAATTATTATATTAAGGTGATTTCATTCTTCTTATAGGACTTGTATATCCATGTTGGCTGGCCACTATACCGGAAATATGGTCATGCTTTTGAGGTATGTTTTTTGGTTTGCGTGCTAAGAGAACAATTTATCTTCTGGAAGAATTTAGGGGCTTGAACTTGAATTGAATTATCTTTTGTGCAGGCATTCAAGGTTATTGTAACTGATCCTGATACAGTGTTGAATGCTCTCACCCGTGAAGTCAAGGAAGCTGGTCCTGATGGGCAAGAGGTAGCATTGGTCCAAGAAAATTGAGGTGCTATTCCCTAAGTTTGGATTACATTCTAACTTAGTCTTGTATTTTAGGTAACTAAGGTAGTTCCCGCAATGTCTGAGGATGTAAAAGATGCGCTAATAAAAAATATTAGAAGAAGAATGACTCCTCAACCCCTGAAGGTTCGGGCTGATATTGAGATGAAGTGTTTCCAGTTTGATGGTGTTCTTCACATTAAGGTCTTTGTTTGACTACTTTTAGCTTCTCTGATTTTTATTTGTTTTCCTCGTTGAAGTTCTTTCATGAAAAATGTAAACACGGACTTATCTAATTTTGCAGGAAGCAATGCGGAAAGCTGAGGCTGCTGGGAATGATGATTGTCCTGTTAAGATTAAATTGGTTGCTCCTCCACTCTATGTTCTTACGACCCAGACACTTGACAAGGTTAGTACAGTCCTCCCATGATGTGATGAATTGTTGTCTGTTATTCATGGAGATATTTGCAATTTGAGATACAAAGAATTTTCCAACTATCAATCTCCTGAACATCTATATATATATATATATATATATTCATCTCTGGTGATATGTACTCTTTAAGAATCTGGATTTTCTAAATTTAATCACTTAAAAAAAGCTTGTTTTCTTATGAACTGACATGGGGCTAGGCAAGCTTTTCTCTAGCATTTTGTGGATGTGAGCTATGAGCATAATGCAAGGAGTGAGCTCTCAAACACCTTGGTTATTTGAAAAGAAAAAATGCTCGAGATGTTCTTAATATTCTTTAATTTGGTGACTTCTTAATTTCTTTCGTCTCCAACCAAAAAGTGTCTCTTCCCCTTTCTTCTTTTGGGATGAGCTTGGCGATGAGAGTAACAGGATTGGTCTCGAGTCTTTAAGATTGAAGGTATTTTTAGATAAGAACCATATATCTGGAATACTGATGACTACATATGCCACCACTTGACGTGTTTAAATTGTTTTCGACTATTTTACGTTTTTCGATTGGCAGCTTTGTTTAAGTTATTCATTTGTTATTTTGTGTTGAATAGGAGCAAGGAATCACTGTTCTTGAAAAAGCAATCACTGCCTGCACGGAGGCAATCGAGCATCACAAGGGAAAGCTTGTAGTGAAGGAGGCACCTAGAGCGGTTAGTCTCAACATCTGTTCACATTTAATATTAATGGTTACATCCGTTTCATCAGCTGACATCCAATCTTGTCCAATTACTATTGTAGGTGAGTGAAAGAGACGACAAATTACTTGCTGAGCACATGGCAAAGCTACGACAAGACAACGAAGAGATCAGTGGCGATGAGGATAGTGAGGAAGAGGAAGACACTGGAATGGGAGAAGTCGATGTCGAAAATGCAGGTTCTGGGATCACCGAGTAAAAGATAGTTGCAGGAATCATTTGGGCTTGTAAGTTGAAGAAGGTCTGTAGTTCTGAGAGCTGTTCAATTCTTTCTTGCCACTTTACCTAGAGATTGACACAATTTTGAGAAAATCCCTTTTTTTTGTGAGGTAAGACACCTATACCTATAATACTTGAGTTATTACAATTGGGTAAATTGGGAAGTCTGTGTATTGCTCTTATTTAATGCTTTTTTCTTGATATTCAATGATTAAGCTTTGTGGAAGTCTTTGTTCAACATTCAAATCATTTTGATTCTGATTTTGACCGTTTTAGACACACATTTTATTTTTCTAGTTATATATATAAAGTTAAGGGGTATTTTTATTAATTTAGATA

mRNA sequence

TATCTTCTCCATCAAACTCCCTCTCTCTCTCTCTCTCTAACAATTAGCCCTAAATTCCCCCAAAAGAAAACCCCATTTCGTCTCTTAAACCCTTCTTCACAGAATCCTTCAATTCTCTGAAATACTCTCTACCATGGCTTCCCATTCCCCTAATCTCGAGTGTCGGATGTACGAAGCCAAGTACCCCGAGGTCGACATGGCCGTCATGATCCAGGTCAAGAACATTGCTGACATGGGCGCTTACGTCTCTCTTCTTGAGTACAACAACATCGAGGGTATGATTCTCTTCTCCGAGCTCTCTCGCCGTCGTATTCGTAGTGTCAGCAGCTTAATTAGGGTCGGTCGAATCGAGCCCGTCATGGTCCTCAGGGTCGATAAGGAGAAGGGGTATATTGATTTGAGTAAGAGGAGGGTTTCTGAGGAGGATATTCAGGCATGTGAGGAGAGGTATAACAAGAGCAAGCTTGTTCACTCTATAATGCGCCATGTTGCTGAGACTGAAAATATTGATTTGGAGGACTTGTATATCCATGTTGGCTGGCCACTATACCGGAAATATGGTCATGCTTTTGAGGCATTCAAGGTTATTGTAACTGATCCTGATACAGTGTTGAATGCTCTCACCCGTGAAGTCAAGGAAGCTGGTCCTGATGGGCAAGAGGTAACTAAGGTAGTTCCCGCAATGTCTGAGGATGTAAAAGATGCGCTAATAAAAAATATTAGAAGAAGAATGACTCCTCAACCCCTGAAGGTTCGGGCTGATATTGAGATGAAGTGTTTCCAGTTTGATGGTGTTCTTCACATTAAGGAAGCAATGCGGAAAGCTGAGGCTGCTGGGAATGATGATTGTCCTGTTAAGATTAAATTGGTTGCTCCTCCACTCTATGTTCTTACGACCCAGACACTTGACAAGGAGCAAGGAATCACTGTTCTTGAAAAAGCAATCACTGCCTGCACGGAGGCAATCGAGCATCACAAGGGAAAGCTTGTAGTGAAGGAGGCACCTAGAGCGGTGAGTGAAAGAGACGACAAATTACTTGCTGAGCACATGGCAAAGCTACGACAAGACAACGAAGAGATCAGTGGCGATGAGGATAGTGAGGAAGAGGAAGACACTGGAATGGGAGAAGTCGATGTCGAAAATGCAGGTTCTGGGATCACCGAGTAAAAGATAGTTGCAGGAATCATTTGGGCTTGTAAGTTGAAGAAGGTCTGTAGTTCTGAGAGCTGTTCAATTCTTTCTTGCCACTTTACCTAGAGATTGACACAATTTTGAGAAAATCCCTTTTTTTTGTGAGGTAAGACACCTATACCTATAATACTTGAGTTATTACAATTGGGTAAATTGGGAAGTCTGTGTATTGCTCTTATTTAATGCTTTTTTCTTGATATTCAATGATTAAGCTTTGTGGAAGTCTTTGTTCAACATTCAAATCATTTTGATTCTGATTTTGACCGTTTTAGACACACATTTTATTTTTCTAGTTATATATATAAAGTTAAGGGGTATTTTTATTAATTTAGATA

Coding sequence (CDS)

ATGGCTTCCCATTCCCCTAATCTCGAGTGTCGGATGTACGAAGCCAAGTACCCCGAGGTCGACATGGCCGTCATGATCCAGGTCAAGAACATTGCTGACATGGGCGCTTACGTCTCTCTTCTTGAGTACAACAACATCGAGGGTATGATTCTCTTCTCCGAGCTCTCTCGCCGTCGTATTCGTAGTGTCAGCAGCTTAATTAGGGTCGGTCGAATCGAGCCCGTCATGGTCCTCAGGGTCGATAAGGAGAAGGGGTATATTGATTTGAGTAAGAGGAGGGTTTCTGAGGAGGATATTCAGGCATGTGAGGAGAGGTATAACAAGAGCAAGCTTGTTCACTCTATAATGCGCCATGTTGCTGAGACTGAAAATATTGATTTGGAGGACTTGTATATCCATGTTGGCTGGCCACTATACCGGAAATATGGTCATGCTTTTGAGGCATTCAAGGTTATTGTAACTGATCCTGATACAGTGTTGAATGCTCTCACCCGTGAAGTCAAGGAAGCTGGTCCTGATGGGCAAGAGGTAACTAAGGTAGTTCCCGCAATGTCTGAGGATGTAAAAGATGCGCTAATAAAAAATATTAGAAGAAGAATGACTCCTCAACCCCTGAAGGTTCGGGCTGATATTGAGATGAAGTGTTTCCAGTTTGATGGTGTTCTTCACATTAAGGAAGCAATGCGGAAAGCTGAGGCTGCTGGGAATGATGATTGTCCTGTTAAGATTAAATTGGTTGCTCCTCCACTCTATGTTCTTACGACCCAGACACTTGACAAGGAGCAAGGAATCACTGTTCTTGAAAAAGCAATCACTGCCTGCACGGAGGCAATCGAGCATCACAAGGGAAAGCTTGTAGTGAAGGAGGCACCTAGAGCGGTGAGTGAAAGAGACGACAAATTACTTGCTGAGCACATGGCAAAGCTACGACAAGACAACGAAGAGATCAGTGGCGATGAGGATAGTGAGGAAGAGGAAGACACTGGAATGGGAGAAGTCGATGTCGAAAATGCAGGTTCTGGGATCACCGAGTAA

Protein sequence

MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRIRSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVAETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKVVPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCPVKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDKLLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE
BLAST of Carg18385 vs. NCBI nr
Match: XP_022941858.1 (eukaryotic translation initiation factor 2 subunit alpha homolog [Cucurbita moschata] >XP_022996088.1 eukaryotic translation initiation factor 2 subunit alpha homolog [Cucurbita maxima] >XP_023543107.1 eukaryotic translation initiation factor 2 subunit alpha homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 665.6 bits (1716), Expect = 9.0e-188
Identity = 343/344 (99.71%), Postives = 344/344 (100.00%), Query Frame = 0

Query: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60
           MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI
Sbjct: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60

Query: 61  RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120
           RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA
Sbjct: 61  RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120

Query: 121 ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKV 180
           ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLN+LTREVKEAGPDGQEVTKV
Sbjct: 121 ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNSLTREVKEAGPDGQEVTKV 180

Query: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240
           VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP
Sbjct: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240

Query: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK 300
           VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK
Sbjct: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK 300

Query: 301 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE 345
           LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE
Sbjct: 301 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE 344

BLAST of Carg18385 vs. NCBI nr
Match: XP_022988418.1 (eukaryotic translation initiation factor 2 subunit alpha homolog [Cucurbita maxima] >XP_023538629.1 eukaryotic translation initiation factor 2 subunit alpha homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 654.1 bits (1686), Expect = 2.7e-184
Identity = 335/344 (97.38%), Postives = 340/344 (98.84%), Query Frame = 0

Query: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60
           MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI
Sbjct: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60

Query: 61  RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120
           RSVSSLI+VGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA
Sbjct: 61  RSVSSLIKVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120

Query: 121 ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKV 180
           ET NIDLEDLY+HVGWPLYRKYGH FEAFK+IVTDPD+VLN+LTREVKEAGPDGQEVTKV
Sbjct: 121 ETMNIDLEDLYVHVGWPLYRKYGHGFEAFKIIVTDPDSVLNSLTREVKEAGPDGQEVTKV 180

Query: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240
           VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEA GNDDCP
Sbjct: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAVGNDDCP 240

Query: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK 300
           VKIKLVAPPLYVLTTQTLDKEQGITVLEKAI ACTEAIEHHKGKLVVKEAPRAVSERDDK
Sbjct: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAIIACTEAIEHHKGKLVVKEAPRAVSERDDK 300

Query: 301 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE 345
           LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE
Sbjct: 301 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE 344

BLAST of Carg18385 vs. NCBI nr
Match: XP_022957012.1 (eukaryotic translation initiation factor 2 subunit alpha homolog [Cucurbita moschata])

HSP 1 Score: 652.5 bits (1682), Expect = 7.9e-184
Identity = 332/344 (96.51%), Postives = 340/344 (98.84%), Query Frame = 0

Query: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60
           MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI
Sbjct: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60

Query: 61  RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120
           RSVSSLI+VGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA
Sbjct: 61  RSVSSLIKVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120

Query: 121 ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKV 180
           ET N+DLEDLY+HVGWPLYRKYGH FEAFK++VTDPD+VLN+LTREVKEAGPDGQEVTKV
Sbjct: 121 ETMNLDLEDLYVHVGWPLYRKYGHGFEAFKIVVTDPDSVLNSLTREVKEAGPDGQEVTKV 180

Query: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240
           VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEA GNDDCP
Sbjct: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAVGNDDCP 240

Query: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK 300
           VKIKLVAPPLYVLTTQTLDKEQGITVLEKAI ACTEAIEHHKGKLVVKEAPRAVSERDDK
Sbjct: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAIIACTEAIEHHKGKLVVKEAPRAVSERDDK 300

Query: 301 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE 345
           LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGE+DVENAGSGITE
Sbjct: 301 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEIDVENAGSGITE 344

BLAST of Carg18385 vs. NCBI nr
Match: XP_004139813.1 (PREDICTED: eukaryotic translation initiation factor 2 subunit alpha-like [Cucumis sativus])

HSP 1 Score: 651.7 bits (1680), Expect = 1.3e-183
Identity = 334/344 (97.09%), Postives = 339/344 (98.55%), Query Frame = 0

Query: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60
           MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI
Sbjct: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60

Query: 61  RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120
           RSVSSLI+VGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA
Sbjct: 61  RSVSSLIKVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120

Query: 121 ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKV 180
           ET NIDLEDLYIHVGWPLYRKYGHAFEAFK+IVTDPD+VLN+LTREVKEAGPDGQE TK+
Sbjct: 121 ETMNIDLEDLYIHVGWPLYRKYGHAFEAFKIIVTDPDSVLNSLTREVKEAGPDGQEETKI 180

Query: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240
           VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP
Sbjct: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240

Query: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK 300
           VKIKLVAPPLYVLTTQTLDKEQGITVLEKAI ACTEAIEHHKGKLVVKE PRAVSERDDK
Sbjct: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAIAACTEAIEHHKGKLVVKETPRAVSERDDK 300

Query: 301 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE 345
           LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAG GITE
Sbjct: 301 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGPGITE 344

BLAST of Carg18385 vs. NCBI nr
Match: KGN44230.1 (hypothetical protein Csa_7G232540 [Cucumis sativus])

HSP 1 Score: 651.7 bits (1680), Expect = 1.3e-183
Identity = 334/344 (97.09%), Postives = 339/344 (98.55%), Query Frame = 0

Query: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60
           MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI
Sbjct: 67  MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 126

Query: 61  RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120
           RSVSSLI+VGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA
Sbjct: 127 RSVSSLIKVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 186

Query: 121 ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKV 180
           ET NIDLEDLYIHVGWPLYRKYGHAFEAFK+IVTDPD+VLN+LTREVKEAGPDGQE TK+
Sbjct: 187 ETMNIDLEDLYIHVGWPLYRKYGHAFEAFKIIVTDPDSVLNSLTREVKEAGPDGQEETKI 246

Query: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240
           VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP
Sbjct: 247 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 306

Query: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK 300
           VKIKLVAPPLYVLTTQTLDKEQGITVLEKAI ACTEAIEHHKGKLVVKE PRAVSERDDK
Sbjct: 307 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAIAACTEAIEHHKGKLVVKETPRAVSERDDK 366

Query: 301 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE 345
           LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAG GITE
Sbjct: 367 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGPGITE 410

BLAST of Carg18385 vs. TAIR10
Match: AT2G40290.1 (Eukaryotic translation initiation factor 2 subunit 1)

HSP 1 Score: 566.2 bits (1458), Expect = 1.3e-161
Identity = 289/345 (83.77%), Postives = 318/345 (92.17%), Query Frame = 0

Query: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60
           MAS +PNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI
Sbjct: 1   MASQTPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60

Query: 61  RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120
           RSVSSLI+VGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQ CEERYNKSKLVHSIMRHVA
Sbjct: 61  RSVSSLIKVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQTCEERYNKSKLVHSIMRHVA 120

Query: 121 ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKV 180
           ET +IDLEDLY+++GWPLYR++GHAFEAFK++VTDPD+VL  LTRE+KE GPDGQEVTKV
Sbjct: 121 ETLSIDLEDLYVNIGWPLYRRHGHAFEAFKILVTDPDSVLGPLTREIKEVGPDGQEVTKV 180

Query: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240
           VPA++E+VKDAL+KNIRRRMTPQP+K+RADIE+KCFQFDGV+HIKEAM+ AEAAGN+DCP
Sbjct: 181 VPAVTEEVKDALVKNIRRRMTPQPMKIRADIELKCFQFDGVVHIKEAMKNAEAAGNEDCP 240

Query: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK 300
           VKIKLVAPPLYVLTTQTLDKEQGI +L KAI ACTE IE HKGKLVVKE  RAVSERD+K
Sbjct: 241 VKIKLVAPPLYVLTTQTLDKEQGIEILNKAIAACTETIETHKGKLVVKEGARAVSERDEK 300

Query: 301 LLAEHMAKLRQDNEEISG-DEDSEEEEDTGMGEVDVENAGSGITE 345
           +L EHMAKLR DNEE+SG         DTGMGEVD++ AG+GI E
Sbjct: 301 MLTEHMAKLRLDNEEMSGXXXXXXXXXDTGMGEVDLD-AGAGIIE 344

BLAST of Carg18385 vs. TAIR10
Match: AT5G05470.1 (eukaryotic translation initiation factor 2 alpha subunit)

HSP 1 Score: 552.4 bits (1422), Expect = 2.0e-157
Identity = 280/345 (81.16%), Postives = 315/345 (91.30%), Query Frame = 0

Query: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60
           MA+ +PNLECRMYE++YP+VDMAVMIQVK IADMGAYVSLLEYNNIEGMILFSELSRRRI
Sbjct: 1   MANPAPNLECRMYESRYPDVDMAVMIQVKTIADMGAYVSLLEYNNIEGMILFSELSRRRI 60

Query: 61  RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120
           RS+SSLI+VGR EPVMVLRVD+E+GYIDLSKRRVS+ED +ACEERYNKSKLVHSIMRHVA
Sbjct: 61  RSISSLIKVGRTEPVMVLRVDRERGYIDLSKRRVSDEDKEACEERYNKSKLVHSIMRHVA 120

Query: 121 ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKV 180
           ET  +DLE+LY+++GWPLY+K+GHAFEAFK++VTDPD+V +ALTREVKE GPDG EVTKV
Sbjct: 121 ETVGVDLEELYVNIGWPLYKKHGHAFEAFKIVVTDPDSVFDALTREVKETGPDGVEVTKV 180

Query: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240
           VPA+SE++KDA +K+IRRRMTPQP+K+RADIE+KCFQFDGVLHIKEAM+KAEA G DDCP
Sbjct: 181 VPAVSEELKDAFLKDIRRRMTPQPMKIRADIELKCFQFDGVLHIKEAMKKAEAVGTDDCP 240

Query: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK 300
           VKIKLVAPPLYVLTT T  KE+GI  L KAI AC  AIE HKGKLVVKE  RAVSERDDK
Sbjct: 241 VKIKLVAPPLYVLTTHTHYKEKGIVTLNKAIEACITAIEEHKGKLVVKEGARAVSERDDK 300

Query: 301 LLAEHMAKLRQDNEEISGDEDSE-EEEDTGMGEVDVENAGSGITE 345
           LLAEHMAKLR DNEE+SGDE SE EEEDTGMGEVD++  GSGI E
Sbjct: 301 LLAEHMAKLRMDNEEMSGDEGSEDEEEDTGMGEVDID-GGSGIIE 344

BLAST of Carg18385 vs. Swiss-Prot
Match: sp|Q9SIZ2|IF2AH_ARATH (Eukaryotic translation initiation factor 2 subunit alpha homolog OS=Arabidopsis thaliana OX=3702 GN=At2g40290 PE=1 SV=2)

HSP 1 Score: 566.2 bits (1458), Expect = 2.4e-160
Identity = 289/345 (83.77%), Postives = 318/345 (92.17%), Query Frame = 0

Query: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60
           MAS +PNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI
Sbjct: 1   MASQTPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60

Query: 61  RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120
           RSVSSLI+VGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQ CEERYNKSKLVHSIMRHVA
Sbjct: 61  RSVSSLIKVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQTCEERYNKSKLVHSIMRHVA 120

Query: 121 ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKV 180
           ET +IDLEDLY+++GWPLYR++GHAFEAFK++VTDPD+VL  LTRE+KE GPDGQEVTKV
Sbjct: 121 ETLSIDLEDLYVNIGWPLYRRHGHAFEAFKILVTDPDSVLGPLTREIKEVGPDGQEVTKV 180

Query: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240
           VPA++E+VKDAL+KNIRRRMTPQP+K+RADIE+KCFQFDGV+HIKEAM+ AEAAGN+DCP
Sbjct: 181 VPAVTEEVKDALVKNIRRRMTPQPMKIRADIELKCFQFDGVVHIKEAMKNAEAAGNEDCP 240

Query: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK 300
           VKIKLVAPPLYVLTTQTLDKEQGI +L KAI ACTE IE HKGKLVVKE  RAVSERD+K
Sbjct: 241 VKIKLVAPPLYVLTTQTLDKEQGIEILNKAIAACTETIETHKGKLVVKEGARAVSERDEK 300

Query: 301 LLAEHMAKLRQDNEEISG-DEDSEEEEDTGMGEVDVENAGSGITE 345
           +L EHMAKLR DNEE+SG         DTGMGEVD++ AG+GI E
Sbjct: 301 MLTEHMAKLRLDNEEMSGXXXXXXXXXDTGMGEVDLD-AGAGIIE 344

BLAST of Carg18385 vs. Swiss-Prot
Match: sp|Q9FE78|IF2A_ARATH (Eukaryotic translation initiation factor 2 subunit alpha OS=Arabidopsis thaliana OX=3702 GN=EIF2A PE=1 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 3.6e-156
Identity = 280/345 (81.16%), Postives = 315/345 (91.30%), Query Frame = 0

Query: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60
           MA+ +PNLECRMYE++YP+VDMAVMIQVK IADMGAYVSLLEYNNIEGMILFSELSRRRI
Sbjct: 1   MANPAPNLECRMYESRYPDVDMAVMIQVKTIADMGAYVSLLEYNNIEGMILFSELSRRRI 60

Query: 61  RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120
           RS+SSLI+VGR EPVMVLRVD+E+GYIDLSKRRVS+ED +ACEERYNKSKLVHSIMRHVA
Sbjct: 61  RSISSLIKVGRTEPVMVLRVDRERGYIDLSKRRVSDEDKEACEERYNKSKLVHSIMRHVA 120

Query: 121 ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKV 180
           ET  +DLE+LY+++GWPLY+K+GHAFEAFK++VTDPD+V +ALTREVKE GPDG EVTKV
Sbjct: 121 ETVGVDLEELYVNIGWPLYKKHGHAFEAFKIVVTDPDSVFDALTREVKETGPDGVEVTKV 180

Query: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240
           VPA+SE++KDA +K+IRRRMTPQP+K+RADIE+KCFQFDGVLHIKEAM+KAEA G DDCP
Sbjct: 181 VPAVSEELKDAFLKDIRRRMTPQPMKIRADIELKCFQFDGVLHIKEAMKKAEAVGTDDCP 240

Query: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK 300
           VKIKLVAPPLYVLTT T  KE+GI  L KAI AC  AIE HKGKLVVKE  RAVSERDDK
Sbjct: 241 VKIKLVAPPLYVLTTHTHYKEKGIVTLNKAIEACITAIEEHKGKLVVKEGARAVSERDDK 300

Query: 301 LLAEHMAKLRQDNEEISGDEDSE-EEEDTGMGEVDVENAGSGITE 345
           LLAEHMAKLR DNEE+SGDE SE EEEDTGMGEVD++  GSGI E
Sbjct: 301 LLAEHMAKLRMDNEEMSGDEGSEDEEEDTGMGEVDID-GGSGIIE 344

BLAST of Carg18385 vs. Swiss-Prot
Match: sp|P56286|IF2A_SCHPO (Eukaryotic translation initiation factor 2 subunit alpha OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tif211 PE=1 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 3.8e-89
Identity = 172/315 (54.60%), Postives = 221/315 (70.16%), Query Frame = 0

Query: 10  CRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRIRSVSSLIRV 69
           CRMYE ++PEVD  V++ V+ I +MGAYV LLEY+NIEGM+L SELSRRRIRSV   IRV
Sbjct: 6   CRMYENRFPEVDELVVVNVRQIQEMGAYVKLLEYDNIEGMVLLSELSRRRIRSVQKHIRV 65

Query: 70  GRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVAETENIDLED 129
           GR E V+VLRVDKEKGYIDLSKRRVS ED+  CEER+NKSK VHSIMRH+AE  N+ LE 
Sbjct: 66  GRNEVVVVLRVDKEKGYIDLSKRRVSPEDVVKCEERFNKSKAVHSIMRHIAEKHNVPLET 125

Query: 130 LYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKVVPAMSEDVK 189
           +Y  +GWPLYRKYGHA++AFK+ +++PD V   L                  P  S  + 
Sbjct: 126 MYTTIGWPLYRKYGHAYDAFKLAISNPDHVFEGLE-----------------PPKSGVIN 185

Query: 190 DALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCPVKIKLVAPP 249
           D L+  I RR+TPQP+K+RAD+E+ CF ++G+  IK A++ AE    ++ P+K+KLVAPP
Sbjct: 186 D-LLAQISRRLTPQPIKIRADVEVTCFGYEGINAIKAALKAAEDVHTEEVPIKVKLVAPP 245

Query: 250 LYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDKLLAEHMAKL 309
           LYVL T  LDK  G+  LE+AI A  ++I    G   VK  P+AVSE D+  LA+ M K 
Sbjct: 246 LYVLLTNALDKSLGLKKLEEAIGAIEKSITASNGTCTVKMKPKAVSETDELELADLMKKF 302

Query: 310 RQDNEEISGDEDSEE 325
            ++N EISGDE+ ++
Sbjct: 306 EKENAEISGDEEDDQ 302

BLAST of Carg18385 vs. Swiss-Prot
Match: sp|Q6GL89|IF2A_XENTR (Eukaryotic translation initiation factor 2 subunit 1 OS=Xenopus tropicalis OX=8364 GN=eif2s1 PE=2 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 5.2e-78
Identity = 158/329 (48.02%), Postives = 226/329 (68.69%), Query Frame = 0

Query: 6   PNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRIRSVSS 65
           P L CR Y+ K+PEVD  VM+ V++IA+MGAYVSLLEYNNIEGMIL SELSRRRIRS++ 
Sbjct: 2   PALSCRFYQHKFPEVDDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINK 61

Query: 66  LIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVAE---- 125
           LIR+GR E V+V+RVDK+KGYIDLSKRRVS E+   CE+++ KSK V+SI+RHVAE    
Sbjct: 62  LIRIGRNECVVVIRVDKDKGYIDLSKRRVSPEEALKCEDKFTKSKTVYSILRHVAEVLEY 121

Query: 126 TENIDLEDLYIHVGWPL---YRKYGH-AFEAFKVIVTDPDTVLNALTREVKEAGPDGQEV 185
           T++  LE LY    W     Y+K G+ A++AFK  V+DPD +             DG + 
Sbjct: 122 TKDEQLESLYQRTAWVFDEKYKKPGYGAYDAFKHAVSDPDVL-------------DGLD- 181

Query: 186 TKVVPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGND 245
                 ++ED +  LI NI RR+TPQ +K+RADIE+ C+ ++G+  +K+A+R   +   +
Sbjct: 182 ------LNEDERRVLIDNINRRLTPQAVKIRADIEVACYGYEGIDAVKDALRAGLSCSTE 241

Query: 246 DCPVKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSER 305
           + P+KI L+APP YV+TT TL++ +G++VL +A++   E IE  +G   V+  P+ V++ 
Sbjct: 242 NMPIKINLIAPPRYVMTTTTLERTEGLSVLNQAMSVIKERIEEKRGVFNVQMEPKVVTDT 301

Query: 306 DDKLLAEHMAKLRQDNEEISGDEDSEEEE 327
           D+  LA  + +L ++N E+ GD+D+EE E
Sbjct: 302 DETELARQLERLEKENAEVDGDDDAEEME 310

BLAST of Carg18385 vs. Swiss-Prot
Match: sp|Q5R493|IF2A_PONAB (Eukaryotic translation initiation factor 2 subunit 1 OS=Pongo abelii OX=9601 GN=EIF2S1 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.5e-77
Identity = 157/329 (47.72%), Postives = 226/329 (68.69%), Query Frame = 0

Query: 6   PNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRIRSVSS 65
           P L CR Y+ K+PEV+  VM+ V++IA+MGAYVSLLEYNNIEGMIL SELSRRRIRS++ 
Sbjct: 2   PGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINK 61

Query: 66  LIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVAE---- 125
           LIR+GR E V+V+RVDKEKGYIDLSKRRVS E+   CE+++ KSK V+SI+RHVAE    
Sbjct: 62  LIRIGRNERVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEY 121

Query: 126 TENIDLEDLYIHVGWPL---YRKYGH-AFEAFKVIVTDPDTVLNALTREVKEAGPDGQEV 185
           T++  LE L+    W     Y++ G+ A++AFK  V+DP ++L++L              
Sbjct: 122 TKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDP-SILDSLD------------- 181

Query: 186 TKVVPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGND 245
                 ++ED ++ LI NI RR+TPQ +K+RADIE+ C+ ++G+  +KEA+R       +
Sbjct: 182 ------LNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTE 241

Query: 246 DCPVKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSER 305
           + P+KI L+APP YV+TT TL++ +G++VL +A+    E IE  +G   V+  P+ V++ 
Sbjct: 242 NMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDT 301

Query: 306 DDKLLAEHMAKLRQDNEEISGDEDSEEEE 327
           D+  LA  M +L ++N E+ GD+D+EE E
Sbjct: 302 DETELARQMERLERENAEVDGDDDAEEME 310

BLAST of Carg18385 vs. TrEMBL
Match: tr|A0A0A0K3F3|A0A0A0K3F3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G232540 PE=4 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 8.9e-184
Identity = 334/344 (97.09%), Postives = 339/344 (98.55%), Query Frame = 0

Query: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60
           MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI
Sbjct: 67  MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 126

Query: 61  RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120
           RSVSSLI+VGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA
Sbjct: 127 RSVSSLIKVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 186

Query: 121 ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKV 180
           ET NIDLEDLYIHVGWPLYRKYGHAFEAFK+IVTDPD+VLN+LTREVKEAGPDGQE TK+
Sbjct: 187 ETMNIDLEDLYIHVGWPLYRKYGHAFEAFKIIVTDPDSVLNSLTREVKEAGPDGQEETKI 246

Query: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240
           VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP
Sbjct: 247 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 306

Query: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK 300
           VKIKLVAPPLYVLTTQTLDKEQGITVLEKAI ACTEAIEHHKGKLVVKE PRAVSERDDK
Sbjct: 307 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAIAACTEAIEHHKGKLVVKETPRAVSERDDK 366

Query: 301 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE 345
           LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAG GITE
Sbjct: 367 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGPGITE 410

BLAST of Carg18385 vs. TrEMBL
Match: tr|W9QN46|W9QN46_9ROSA (Eukaryotic translation initiation factor 2 subunit alpha OS=Morus notabilis OX=981085 GN=L484_006929 PE=4 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 6.2e-177
Identity = 321/344 (93.31%), Postives = 333/344 (96.80%), Query Frame = 0

Query: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60
           MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI
Sbjct: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60

Query: 61  RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120
           RSVSSLI+VGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA
Sbjct: 61  RSVSSLIKVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120

Query: 121 ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKV 180
           ET  IDLEDLYIHVGWPLYRKY HAFEAFK+IVTDPD+VLN+LTREVKE G DGQEVTKV
Sbjct: 121 ETMGIDLEDLYIHVGWPLYRKYSHAFEAFKIIVTDPDSVLNSLTREVKEIGHDGQEVTKV 180

Query: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240
           VPAM+E+VKDALIKNIRRRMTPQPLK+RAD+EMKCFQFDGVLHIKEAMRKAEAAGN+DCP
Sbjct: 181 VPAMTEEVKDALIKNIRRRMTPQPLKIRADVEMKCFQFDGVLHIKEAMRKAEAAGNNDCP 240

Query: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK 300
           VKIKLVAPPLYVLTTQTLDKEQGI VL  AI ACT+AIE HKGKL VKEAPRAVSERDDK
Sbjct: 241 VKIKLVAPPLYVLTTQTLDKEQGIAVLNNAIIACTQAIEQHKGKLAVKEAPRAVSERDDK 300

Query: 301 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE 345
           LLAEHMAKLRQDNEE+SGDEDSEEEEDTGMGEVDVENAG+GITE
Sbjct: 301 LLAEHMAKLRQDNEEVSGDEDSEEEEDTGMGEVDVENAGTGITE 344

BLAST of Carg18385 vs. TrEMBL
Match: tr|A0A2I4DVP5|A0A2I4DVP5_9ROSI (eukaryotic translation initiation factor 2 subunit alpha homolog OS=Juglans regia OX=51240 GN=LOC108983888 PE=4 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 7.6e-175
Identity = 317/344 (92.15%), Postives = 333/344 (96.80%), Query Frame = 0

Query: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60
           MASH+PN ECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI
Sbjct: 1   MASHTPNFECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60

Query: 61  RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120
           RSVSSLI+VGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA
Sbjct: 61  RSVSSLIKVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120

Query: 121 ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKV 180
           ET +IDLEDLYIHVGWPLYRKYGHAFEAFK++VTDPD+VLN+LTREVKE  PDGQEVTKV
Sbjct: 121 ETMSIDLEDLYIHVGWPLYRKYGHAFEAFKIMVTDPDSVLNSLTREVKEISPDGQEVTKV 180

Query: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240
           VPAMSE+VK+ALIKNIRRRMTPQPLK+RADIEMKCFQFDGV+HIK+AMR+AEAAGNDDCP
Sbjct: 181 VPAMSEEVKEALIKNIRRRMTPQPLKIRADIEMKCFQFDGVIHIKDAMRQAEAAGNDDCP 240

Query: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK 300
           VKIKLVAPPLYVLTTQTLDKEQGI VL +AI ACTEAIE  KGKL VKEA RAVSERDDK
Sbjct: 241 VKIKLVAPPLYVLTTQTLDKEQGIAVLNRAIIACTEAIEKQKGKLAVKEAARAVSERDDK 300

Query: 301 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE 345
           LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAG+GIT+
Sbjct: 301 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGTGITD 344

BLAST of Carg18385 vs. TrEMBL
Match: tr|A0A151SNX2|A0A151SNX2_CAJCA (Eukaryotic translation initiation factor 2 subunit alpha OS=Cajanus cajan OX=3821 GN=KK1_002724 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 1.3e-174
Identity = 317/340 (93.24%), Postives = 331/340 (97.35%), Query Frame = 0

Query: 5   SPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRIRSVS 64
           +PNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRIRSVS
Sbjct: 2   APNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRIRSVS 61

Query: 65  SLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVAETEN 124
           SLI+VGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVAET N
Sbjct: 62  SLIKVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVAETLN 121

Query: 125 IDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKVVPAM 184
           IDLE+LYIH+GWPLYRKYGHAFEAFK+IVTDPDTVLN LTREVKE GPDGQEVTKVVPA+
Sbjct: 122 IDLEELYIHIGWPLYRKYGHAFEAFKIIVTDPDTVLNTLTREVKEVGPDGQEVTKVVPAV 181

Query: 185 SEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCPVKIK 244
           SE+VKD+L+KNIRRRMTPQPLK+RADIEMKCFQFDGV+HIKEAMRKAEAAGNDDCPVKIK
Sbjct: 182 SEEVKDSLVKNIRRRMTPQPLKIRADIEMKCFQFDGVIHIKEAMRKAEAAGNDDCPVKIK 241

Query: 245 LVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDKLLAE 304
           LVAPPLYVLTTQTLDKEQGI VL KAIT+CTEAIE HKGKLVVKEAPRAVSERDDKLLAE
Sbjct: 242 LVAPPLYVLTTQTLDKEQGILVLNKAITSCTEAIEQHKGKLVVKEAPRAVSERDDKLLAE 301

Query: 305 HMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE 345
           HMAKLRQDNEE+SGDEDSEE EDTGMGEVDV+N G GITE
Sbjct: 302 HMAKLRQDNEEVSGDEDSEEXEDTGMGEVDVDN-GPGITE 340

BLAST of Carg18385 vs. TrEMBL
Match: tr|A0A2P4LV43|A0A2P4LV43_QUESU (Eukaryotic translation initiation factor 2 subunit alpha like OS=Quercus suber OX=58331 GN=CFP56_56795 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 1.7e-174
Identity = 320/344 (93.02%), Postives = 332/344 (96.51%), Query Frame = 0

Query: 1   MASHSPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60
           MAS +PNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI
Sbjct: 1   MASQTPNLECRMYEAKYPEVDMAVMIQVKNIADMGAYVSLLEYNNIEGMILFSELSRRRI 60

Query: 61  RSVSSLIRVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120
           RSVSSLI+VGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA
Sbjct: 61  RSVSSLIKVGRIEPVMVLRVDKEKGYIDLSKRRVSEEDIQACEERYNKSKLVHSIMRHVA 120

Query: 121 ETENIDLEDLYIHVGWPLYRKYGHAFEAFKVIVTDPDTVLNALTREVKEAGPDGQEVTKV 180
           ET +IDLEDLYIHVGWPLYRKYGHAFEAFK+IVTDPDTVLN LTREVKE GPDGQEVTKV
Sbjct: 121 ETMSIDLEDLYIHVGWPLYRKYGHAFEAFKIIVTDPDTVLNTLTREVKEIGPDGQEVTKV 180

Query: 181 VPAMSEDVKDALIKNIRRRMTPQPLKVRADIEMKCFQFDGVLHIKEAMRKAEAAGNDDCP 240
           VPA+SE+VKD+LIKNIRRRMTPQPLK+RADIEMKCFQFDGVLHIK+AMRKAEAAGNDDCP
Sbjct: 181 VPALSEEVKDSLIKNIRRRMTPQPLKIRADIEMKCFQFDGVLHIKDAMRKAEAAGNDDCP 240

Query: 241 VKIKLVAPPLYVLTTQTLDKEQGITVLEKAITACTEAIEHHKGKLVVKEAPRAVSERDDK 300
           VKIKLVAPPLYVLTTQTLDKEQGI VL +AI ACTEAIE HKGKL VKEAPRAVSERDDK
Sbjct: 241 VKIKLVAPPLYVLTTQTLDKEQGIAVLNRAIIACTEAIEQHKGKLGVKEAPRAVSERDDK 300

Query: 301 LLAEHMAKLRQDNEEISGDEDSEEEEDTGMGEVDVENAGSGITE 345
           LLAEHMAKLR+ NEE+SGDEDSEEEEDTGMGEVDVENA  GITE
Sbjct: 301 LLAEHMAKLREANEELSGDEDSEEEEDTGMGEVDVENA--GITE 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022941858.19.0e-18899.71eukaryotic translation initiation factor 2 subunit alpha homolog [Cucurbita mosc... [more]
XP_022988418.12.7e-18497.38eukaryotic translation initiation factor 2 subunit alpha homolog [Cucurbita maxi... [more]
XP_022957012.17.9e-18496.51eukaryotic translation initiation factor 2 subunit alpha homolog [Cucurbita mosc... [more]
XP_004139813.11.3e-18397.09PREDICTED: eukaryotic translation initiation factor 2 subunit alpha-like [Cucumi... [more]
KGN44230.11.3e-18397.09hypothetical protein Csa_7G232540 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT2G40290.11.3e-16183.77Eukaryotic translation initiation factor 2 subunit 1[more]
AT5G05470.12.0e-15781.16eukaryotic translation initiation factor 2 alpha subunit[more]
Match NameE-valueIdentityDescription
sp|Q9SIZ2|IF2AH_ARATH2.4e-16083.77Eukaryotic translation initiation factor 2 subunit alpha homolog OS=Arabidopsis ... [more]
sp|Q9FE78|IF2A_ARATH3.6e-15681.16Eukaryotic translation initiation factor 2 subunit alpha OS=Arabidopsis thaliana... [more]
sp|P56286|IF2A_SCHPO3.8e-8954.60Eukaryotic translation initiation factor 2 subunit alpha OS=Schizosaccharomyces ... [more]
sp|Q6GL89|IF2A_XENTR5.2e-7848.02Eukaryotic translation initiation factor 2 subunit 1 OS=Xenopus tropicalis OX=83... [more]
sp|Q5R493|IF2A_PONAB1.5e-7747.72Eukaryotic translation initiation factor 2 subunit 1 OS=Pongo abelii OX=9601 GN=... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0K3F3|A0A0A0K3F3_CUCSA8.9e-18497.09Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G232540 PE=4 SV=1[more]
tr|W9QN46|W9QN46_9ROSA6.2e-17793.31Eukaryotic translation initiation factor 2 subunit alpha OS=Morus notabilis OX=9... [more]
tr|A0A2I4DVP5|A0A2I4DVP5_9ROSI7.6e-17592.15eukaryotic translation initiation factor 2 subunit alpha homolog OS=Juglans regi... [more]
tr|A0A151SNX2|A0A151SNX2_CAJCA1.3e-17493.24Eukaryotic translation initiation factor 2 subunit alpha OS=Cajanus cajan OX=382... [more]
tr|A0A2P4LV43|A0A2P4LV43_QUESU1.7e-17493.02Eukaryotic translation initiation factor 2 subunit alpha like OS=Quercus suber O... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
GO:0003723RNA binding
GO:0003743translation initiation factor activity
Vocabulary: INTERPRO
TermDefinition
IPR012340NA-bd_OB-fold
IPR024055TIF2_asu_C
IPR024054TIF2_asu_middle_sf
IPR003029S1_domain
IPR011488TIF_2_asu
IPR022967S1_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006446 regulation of translational initiation
cellular_component GO:0005840 ribosome
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003723 RNA binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg18385-RACarg18385-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 306..326
NoneNo IPR availableGENE3DG3DSA:2.40.50.140coord: 6..94
e-value: 2.7E-38
score: 131.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 316..332
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 309..344
NoneNo IPR availableCDDcd04452S1_IF2_alphacoord: 18..93
e-value: 7.99636E-31
score: 111.522
IPR022967RNA-binding domain, S1SMARTSM00316S1_6coord: 19..92
e-value: 1.1E-10
score: 51.5
IPR011488Translation initiation factor 2, alpha subunitPFAMPF07541EIF_2_alphacoord: 130..260
e-value: 9.9E-40
score: 135.2
IPR011488Translation initiation factor 2, alpha subunitPANTHERPTHR10602EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1coord: 7..334
IPR003029S1 domainPFAMPF00575S1coord: 18..92
e-value: 1.0E-12
score: 48.1
IPR003029S1 domainPROSITEPS50126S1coord: 21..92
score: 15.926
IPR024054Translation initiation factor 2, alpha subunit, middle domain superfamilyGENE3DG3DSA:1.10.150.190coord: 95..199
e-value: 1.0E-29
score: 104.4
IPR024054Translation initiation factor 2, alpha subunit, middle domain superfamilySUPERFAMILYSSF116742eIF2alpha middle domain-likecoord: 94..199
IPR024055Translation initiation factor 2, alpha subunit, C-terminalGENE3DG3DSA:3.30.70.1130coord: 204..327
e-value: 5.9E-42
score: 144.3
IPR024055Translation initiation factor 2, alpha subunit, C-terminalSUPERFAMILYSSF110993eIF-2-alpha, C-terminal domaincoord: 203..319
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILYSSF50249Nucleic acid-binding proteinscoord: 15..97