Carg18054 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg18054
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Calcium-binding EF-hand family protein
Location	Cucurbita_argyrosperma_scaffold_095 : 140706 .. 141053 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCGTAGAATTACTAGACAGCGCCACCGTAGTGAGCTTCGTGGAGGACGAGGAAGCCTTCGGCGCCGCCATTAGGGAGCGTTTCAGGCGCCTCGATGTGGACGGCGATGGCGCGCTGTCGTACACAGAGATGTTGAAGGAGTTTGGAAGCGATGAGGTTTGTTGTGCGTACGAAAGTGAGTCGAATGGAAAAGTTGATTTGGAGAAGTTCATGAGGGGAATGAAGAAGATGATGATGGCCATTGCTGAGGGATTAGGGTTCTTGCCGATTCATATGGCGCTGCAAAAAGATGGGTTGTTGATGAAAGCTGTTCAGAGAGAAGCCGCTGCCATGGTAGATGCTTGA

mRNA sequence

ATGAGCGTAGAATTACTAGACAGCGCCACCGTAGTGAGCTTCGTGGAGGACGAGGAAGCCTTCGGCGCCGCCATTAGGGAGCGTTTCAGGCGCCTCGATGTGGACGGCGATGGCGCGCTGTCGTACACAGAGATGTTGAAGGAGTTTGGAAGCGATGAGGTTTGTTGTGCGTACGAAAGTGAGTCGAATGGAAAAGTTGATTTGGAGAAGTTCATGAGGGGAATGAAGAAGATGATGATGGCCATTGCTGAGGGATTAGGGTTCTTGCCGATTCATATGGCGCTGCAAAAAGATGGGTTGTTGATGAAAGCTGTTCAGAGAGAAGCCGCTGCCATGGTAGATGCTTGA

Coding sequence (CDS)

ATGAGCGTAGAATTACTAGACAGCGCCACCGTAGTGAGCTTCGTGGAGGACGAGGAAGCCTTCGGCGCCGCCATTAGGGAGCGTTTCAGGCGCCTCGATGTGGACGGCGATGGCGCGCTGTCGTACACAGAGATGTTGAAGGAGTTTGGAAGCGATGAGGTTTGTTGTGCGTACGAAAGTGAGTCGAATGGAAAAGTTGATTTGGAGAAGTTCATGAGGGGAATGAAGAAGATGATGATGGCCATTGCTGAGGGATTAGGGTTCTTGCCGATTCATATGGCGCTGCAAAAAGATGGGTTGTTGATGAAAGCTGTTCAGAGAGAAGCCGCTGCCATGGTAGATGCTTGA

Protein sequence

MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA

Homology

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BLAST of Carg18054 vs. NCBI nr
Match: XP_022930694.1 (uncharacterized protein LOC111437091 [Cucurbita moschata] >XP_023530313.1 uncharacterized protein LOC111792919 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 219.9 bits (559), Expect = 4.4e-54
Identity = 113/115 (98.26%), Postives = 114/115 (99.13%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES 60
           MSVELLDSATVVSFVEDEEAFGAAIRERFR LDVDGDGALSYTEMLKEFGSDEVCCAYE+
Sbjct: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRHLDVDGDGALSYTEMLKEFGSDEVCCAYET 60

Query: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA 116
           ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA
Sbjct: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA 115

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BLAST of Carg18054 vs. NCBI nr
Match: XP_008455505.1 (PREDICTED: uncharacterized protein LOC103495656 [Cucumis melo])

HSP 1 Score: 133.7 bits (335), Expect = 4.1e-28
Identity = 76/132 (57.58%), Postives = 89/132 (67.42%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEF----------- 60
           MSVELLD AT+V+FVEDEEAF    RE F RLD D DG LSY EMLKEF           
Sbjct: 1   MSVELLDGATIVNFVEDEEAFNGWTREHFTRLDTDRDGFLSYAEMLKEFYTLRVSEADFG 60

Query: 61  -----GSDEVCCAY-------ESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 110
                 +DE+   Y       + +S+GKVD+++FM GMK MM+AIAEG+GFLPI MAL+K
Sbjct: 61  TDVKLDADELSQLYGAVFKQFDRDSDGKVDVDEFMMGMKNMMLAIAEGMGFLPIQMALEK 120

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BLAST of Carg18054 vs. NCBI nr
Match: XP_004144609.1 (PREDICTED: uncharacterized protein LOC101208968 [Cucumis sativus])

HSP 1 Score: 133.3 bits (334), Expect = 5.4e-28
Identity = 76/132 (57.58%), Postives = 89/132 (67.42%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKE----------FG 60
           MSVELLD AT+V+FVEDEEAF    RE F RLD D DG LSY EMLKE          FG
Sbjct: 1   MSVELLDGATIVNFVEDEEAFNGWTREHFTRLDTDRDGFLSYAEMLKELHALRVSEADFG 60

Query: 61  SD-------------EVCCAYESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 110
           +D              V   ++ +S+GKVD+++FM GMK MM+AIAEG+GFLPI MAL+K
Sbjct: 61  TDVKPDADELSQLYSAVFKQFDRDSDGKVDVDEFMMGMKNMMLAIAEGMGFLPIQMALEK 120

[Close]

BLAST of Carg18054 vs. NCBI nr
Match: XP_022135904.1 (uncharacterized protein LOC111007741 [Momordica charantia])

HSP 1 Score: 131.7 bits (330), Expect = 1.6e-27
Identity = 73/131 (55.73%), Postives = 90/131 (68.70%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKE----------FG 60
           MSVE+LDSAT+++FVEDEEAFGA IRERFR LD D DG LSY EML+E          FG
Sbjct: 1   MSVEILDSATIINFVEDEEAFGAWIRERFRHLDADRDGVLSYAEMLQELHSLRVYETHFG 60

Query: 61  SD-------------EVCCAYESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 109
            D              V   ++  SNG+VDLE+FM G K++M+AIAEG+GFLP+ MAL++
Sbjct: 61  VDAKPDPDELSRLYGAVFMQFDRNSNGEVDLEEFMAGTKRIMVAIAEGMGFLPLQMALEE 120

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BLAST of Carg18054 vs. NCBI nr
Match: XP_021669195.1 (uncharacterized protein LOC110656634 [Hevea brasiliensis])

HSP 1 Score: 121.3 bits (303), Expect = 2.1e-24
Identity = 70/134 (52.24%), Postives = 89/134 (66.42%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKE----------FG 60
           MSVE+LD AT+V+FVEDEEAF  +IRERF  LD+D DG LSYTEMLKE          FG
Sbjct: 1   MSVEVLDGATIVNFVEDEEAFTMSIRERFAHLDIDQDGLLSYTEMLKELQSLRVFETHFG 60

Query: 61  SD------EVCCAYES-------ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 112
            D      E+   Y S       + NG+VDLE+F +  KKMM+A+A G+GFLP+ M L++
Sbjct: 61  IDVKREPEELARVYSSLFEQFDHDLNGRVDLEEFKQETKKMMLAMANGMGFLPVQMVLEE 120

[Close]

BLAST of Carg18054 vs. TAIR10
Match: AT2G44310.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 81.6 bits (200), Expect = 3.4e-16
Identity = 56/140 (40.00%), Postives = 76/140 (54.29%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGS--------- 60
           M V L+D +TV SFV+DEE F  ++ ERF  LD++ DG LS +E+ K F S         
Sbjct: 1   MGVVLIDGSTVRSFVDDEEQFKKSVDERFAALDLNKDGVLSRSELRKAFESMRLLESHFG 60

Query: 61  -------DEVCCAYES-------ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMAL-- 116
                  DE+   Y+S       + +G VDLE+F   MKK+++AIA+GLG  PI M L  
Sbjct: 61  VDVVTPQDELTNLYDSIFEKFDTDQSGSVDLEEFRSEMKKIVLAIADGLGSCPITMVLDD 120

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BLAST of Carg18054 vs. TAIR10
Match: AT1G54530.1 (Calcium-binding EF hand family protein)

HSP 1 Score: 48.5 bits (114), Expect = 3.2e-06
Identity = 32/119 (26.89%), Postives = 62/119 (52.10%), Query Frame = 0


Query: 5   LLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGS-----DEVCCAYE 64
           ++ S T++ F+ D ++F +   + F+ LD+D +G LS +E+ +   +      EV    E
Sbjct: 5   VITSKTLIGFLSDTKSFESITNDYFQILDLDKNGMLSPSELRQGLNNVVAVESEVAPGDE 64

Query: 65  SESNGKVDLEKFMRGM---------KKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREA 110
           +++      E+F   +          +++ A+A G+G  P+ M +  DGL+MKAV  E+
Sbjct: 65  TDNVYNAIFERFGEDLVPKNFRDLIAEILTAMARGIGNSPVIMVVHNDGLIMKAVLHES 123

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BLAST of Carg18054 vs. TAIR10
Match: AT4G38810.2 (Calcium-binding EF-hand family protein)

HSP 1 Score: 42.7 bits (99), Expect = 1.7e-04
Identity = 32/116 (27.59%), Postives = 56/116 (48.28%), Query Frame = 0


Query: 4   ELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEM---LKEFGS--------- 63
           ++LD + +V  VE+E+ F   + ++F++LD D DG LS TE+   + + G+         
Sbjct: 13  QVLDGSDIVELVENEKVFDKFVEQKFQQLDQDEDGKLSVTELQPAVADIGAALGLPAQGT 72

Query: 64  --------DEVCCAYESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDG 100
                    EV   +   S  KV   +F   +  +++ +A GL   PI + L+ DG
Sbjct: 73  SPDSDHIYSEVLNEFTHGSQEKVSKTEFKEVLSDILLGMAAGLKRDPI-VILRMDG 127

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BLAST of Carg18054 vs. TrEMBL
Match: tr|A0A1S3C0M6|A0A1S3C0M6_CUCME (uncharacterized protein LOC103495656 OS=Cucumis melo OX=3656 GN=LOC103495656 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.7e-28
Identity = 76/132 (57.58%), Postives = 89/132 (67.42%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEF----------- 60
           MSVELLD AT+V+FVEDEEAF    RE F RLD D DG LSY EMLKEF           
Sbjct: 1   MSVELLDGATIVNFVEDEEAFNGWTREHFTRLDTDRDGFLSYAEMLKEFYTLRVSEADFG 60

Query: 61  -----GSDEVCCAY-------ESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 110
                 +DE+   Y       + +S+GKVD+++FM GMK MM+AIAEG+GFLPI MAL+K
Sbjct: 61  TDVKLDADELSQLYGAVFKQFDRDSDGKVDVDEFMMGMKNMMLAIAEGMGFLPIQMALEK 120

[Close]

BLAST of Carg18054 vs. TrEMBL
Match: tr|F6HIA0|F6HIA0_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_12s0059g00370 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 4.1e-24
Identity = 69/135 (51.11%), Postives = 85/135 (62.96%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGS--------- 60
           MSVE+LD AT+VSFVEDEEAF   I +RF  LD + DG LSY EMLKEF S         
Sbjct: 1   MSVEVLDGATIVSFVEDEEAFNGLIHDRFSNLDTNHDGFLSYAEMLKEFQSLRVFETHFG 60

Query: 61  -------DEVCCAYES-------ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 113
                  DE+   Y+S       ++NG VDLE+F +  K+MM+A+A GLGFLP+ M L++
Sbjct: 61  IDVKRDPDELAHVYDSLFVKFDHDANGAVDLEEFRKETKQMMLAMANGLGFLPVQMVLEE 120

[Close]

BLAST of Carg18054 vs. TrEMBL
Match: tr|F6HI98|F6HI98_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_12s0059g00350 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 5.3e-24
Identity = 68/135 (50.37%), Postives = 86/135 (63.70%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGS--------- 60
           MSVE+LD AT+VSFVEDEEAF   I +RF  LD + DG LS+TEMLKEF S         
Sbjct: 1   MSVEVLDGATIVSFVEDEEAFNGLIHDRFSNLDTNHDGFLSFTEMLKEFQSLRVFETHFG 60

Query: 61  -------DEVCCAYES-------ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 113
                  DE+   Y+S       ++NG VDLE+F +  K+MM+A+A GLGFLP+ M L++
Sbjct: 61  IDVKRDPDELAHVYDSLFVKFDHDANGAVDLEEFKKETKRMMLAMANGLGFLPVQMVLEE 120

[Close]

BLAST of Carg18054 vs. TrEMBL
Match: tr|V4U9W3|V4U9W3_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10018069mg PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 6.9e-24
Identity = 68/138 (49.28%), Postives = 87/138 (63.04%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKE----------FG 60
           MSVE+LDSAT+VSFVEDEEAF  +IR RF  LD D DG LSY EM++E          FG
Sbjct: 1   MSVEVLDSATIVSFVEDEEAFNNSIRNRFAHLDTDHDGLLSYAEMMQELKSLRVFETHFG 60

Query: 61  SDE-------------VCCAYESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 116
            DE             +   ++ +SNG VDLE+F    K+MM+A+A GLGFLP+ M L++
Sbjct: 61  IDEKPDPEELASVYNSLFVQFDHDSNGTVDLEEFKAETKQMMLAMANGLGFLPVQMVLEE 120

[Close]

BLAST of Carg18054 vs. TrEMBL
Match: tr|A0A2H5PLI2|A0A2H5PLI2_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_147600 PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 6.9e-24
Identity = 68/138 (49.28%), Postives = 87/138 (63.04%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKE----------FG 60
           MSVE+LDSAT+VSFVEDEEAF  +IR RF  LD D DG LSY EM++E          FG
Sbjct: 1   MSVEVLDSATIVSFVEDEEAFNNSIRNRFAHLDTDHDGLLSYAEMMQELKSLRVFETHFG 60

Query: 61  SDE-------------VCCAYESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 116
            DE             +   ++ +SNG VDLE+F    K+MM+A+A GLGFLP+ M L++
Sbjct: 61  IDEKPDPEELASVYNSLFVQFDHDSNGTVDLEEFKAETKQMMLAMANGLGFLPVQMVLEE 120

The following BLAST results are available for this feature:

BLAST of Carg18054 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022930694.1	4.4e-54	98.26	uncharacterized protein LOC111437091 [Cucurbita moschata] >XP_023530313.1 unchar...	[more]
XP_008455505.1	4.1e-28	57.58	PREDICTED: uncharacterized protein LOC103495656 [Cucumis melo]	[more]
XP_004144609.1	5.4e-28	57.58	PREDICTED: uncharacterized protein LOC101208968 [Cucumis sativus]	[more]
XP_022135904.1	1.6e-27	55.73	uncharacterized protein LOC111007741 [Momordica charantia]	[more]
XP_021669195.1	2.1e-24	52.24	uncharacterized protein LOC110656634 [Hevea brasiliensis]	[more]

BLAST of Carg18054 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G44310.1	3.4e-16	40.00	Calcium-binding EF-hand family protein	[more]
AT1G54530.1	3.2e-06	26.89	Calcium-binding EF hand family protein	[more]
AT4G38810.2	1.7e-04	27.59	Calcium-binding EF-hand family protein	[more]

BLAST of Carg18054 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Carg18054 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3C0M6\|A0A1S3C0M6_CUCME	2.7e-28	57.58	uncharacterized protein LOC103495656 OS=Cucumis melo OX=3656 GN=LOC103495656 PE=...	[more]
tr\|F6HIA0\|F6HIA0_VITVI	4.1e-24	51.11	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_12s0059g00370 PE=4 SV=...	[more]
tr\|F6HI98\|F6HI98_VITVI	5.3e-24	50.37	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_12s0059g00350 PE=4 SV=...	[more]
tr\|V4U9W3\|V4U9W3_9ROSI	6.9e-24	49.28	Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10018069mg PE=4 ...	[more]
tr\|A0A2H5PLI2\|A0A2H5PLI2_CITUN	6.9e-24	49.28	Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_147600 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011992	EF-hand-dom_pair
IPR002048	EF_hand_dom
IPR018247	EF_Hand_1_Ca_BS

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg18054-RA	Carg18054-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 4..83 e-value: 1.3E-7 score: 33.9
None	No IPR available	PANTHER	PTHR44089:SF4	SUBFAMILY NOT NAMED	coord: 1..51
None	No IPR available	PANTHER	PTHR44089:SF4	SUBFAMILY NOT NAMED	coord: 57..112
None	No IPR available	PANTHER	PTHR44089	FAMILY NOT NAMED	coord: 1..51 coord: 57..112
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 33..45
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 20..55 score: 8.711
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 63..81 score: 5.726
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 19..86

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg18054	Cla015650	Watermelon (97103) v1	carwmB1066
Carg18054	ClCG02G002450	Watermelon (Charleston Gray)	carwcgB1037

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg18054	Wax gourd	carwgoB1253
Carg18054	Silver-seed gourd	carcarB242
Carg18054	Cucumber (Gy14) v2	carcgybB1049
Carg18054	Cucumber (Gy14) v1	carcgyB1185
Carg18054	Cucurbita maxima (Rimu)	carcmaB1471
Carg18054	Cucurbita maxima (Rimu)	carcmaB1474
Carg18054	Cucurbita moschata (Rifu)	carcmoB1428
Carg18054	Cucurbita moschata (Rifu)	carcmoB1430
Carg18054	Cucurbita moschata (Rifu)	carcmoB1431
Carg18054	Cucurbita pepo (Zucchini)	carcpeB1481
Carg18054	Cucurbita pepo (Zucchini)	carcpeB1482
Carg18054	Cucurbita pepo (Zucchini)	carcpeB1483
Carg18054	Wild cucumber (PI 183967)	carcpiB1103
Carg18054	Cucumber (Chinese Long) v2	carcuB1081
Carg18054	Cucumber (Chinese Long) v3	carcucB1114
Carg18054	Bottle gourd (USVL1VR-Ls)	carlsiB972
Carg18054	Bottle gourd (USVL1VR-Ls)	carlsiB974
Carg18054	Melon (DHL92) v3.5.1	carmeB1033
Carg18054	Melon (DHL92) v3.6.1	carmedB1047
Carg18054	Watermelon (97103) v2	carwmbB1124