Carg17543 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg17543
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Chaperone protein DnaJ
Location	Cucurbita_argyrosperma_scaffold_183 : 128760 .. 132093 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATCAACTTCCCGCGAACGCCGACAATGGAGGCGCTGTATCTCTCCACTTCAGCACCAACCGTTCTTCCATTTCGGAAAACCGTTGGCTCTCGAACCATTTCAAAATCCTCGATTCTCTTCCCTTCTTCATCAAAGTCTCGATTCGCAACGATTTGCAGGCAAAGAATTGATGGAGAAGATACGCCTCTCTCCAATGCTATGGATGCTTACAGATTGTTCGGCGTCGACCCGACCTGCTCCGAATCCGAGCTCAAAGCCGCTTTTCGAGCTAAAGTACGTAGGTTTTACTCGTTGCGATGAAAAATCGGATTGTAGTATCTAGATTTGAGTTTTCGGAATATGATTGGGAAGCGATTAAGTGATTGGCTACTTAGGTTGATTGTAGAATTTTTGGTTTTCTGTACCGATTTTCAATTCGGCTTCGGATTTTTGGCCGTGCAGGTGAAGCAATTCCATCCAGACGTGAATAGAGATGGAAATTATTCCGATTCTATGATTCGTCGTGTAATTCAGGCGTACGAGGTAGCTATTCATTTATAGTATCAAGAAGAAAGTTGTGTGGTGTTCTTATCTCATTGGTGAACGTTGTTTCTTTAGTAGGATGAACATTATGGCAATCATCATTCATGATGACCTTAATTTGATTGTTATCTCCGCGTCCTTTATTACATTGTAGAATTCAAAACTATTGTTCTGTTGTTTCGAGAGAAAGTGCATAGAGAGTAATCTACGAATGGAAAAGATCACCATTAAAGGTTAACGATGAAATATTCAAGCCATAAGTACTTGTTTCGATGCTGACACCACAAGAGACTGTAACAAGCTGGAACTATTTGTGTTCTGATGAAGAACTTGTTCACTTCTATCATCTATGTTATAGTCAATTATGAAACTCAACAATCACTATGCTTTCTACAATATGCAGATGTTGTCAAGTTGCAGCCGAACCGAGTTCATTGAGAGGTATCGTTGTGCTTTTGGTGTTGTTCTTAATGTGTATAGAGCTGCTTAAAATTCTTGCAGCATTCATCATAGAATAAATTGTGTAATGACCATATTCTTGGAAAGTGCAGGGGAATTCTTATTTATAAATGAAAGGTTATAGATAATATGTAACTGTGAGATCCCACCTCGGTTGGAGAGGTAGAGAGGAGAATGAAGCATACTTTATATAGGTGTGGAAACCTCTCCTTAGCATACATGTTTTAAACACCTTGAGAGGAAGCCCGACTGAGAAAGCTCAAAGAGGACAATATCTGTCAGCGGTGGGCTTGAGCTATTACAAATGGTATCAGAGCTATATACGGGGCAATGTGCCAGTGAGAAGGTTGAGCCCCAAAGAGGGGTGGGCACGAGACGGTATGCTAGCAAGGACGCTAGGCCCTGAAGAGGGTAGATTGGGGGGTCTCACATTGATTGGAGAAGGGAACGAGTGCCGGTCAGGATGCTGGACCCCTAAGGGGTAGATTGTGAGATCCCATCGGTTCAGGAGGAGAATGAAGGACCCTTTATAAAGGTGTGGAAACCTTTCACTAGCAGATGCGTTTTAAAAACTAGCCTGGAAGGGAAAGCCCAAAGAGGACGATCTGCCCGCAGTGAGCTTGGGCTATTACAACAACAATGAATTACTTTTTACTCAAAATTTACCATATATTAGGGAGATTAAAGGTCCATTATTGACATTTTATAATTTGATAATGTTCATTTCTATCATTATTCGATGATATATCTATTGTTCATTTTTTATGGAACTCTCACAATCAAACATATGAACTCAGGGAATGTTTAGATCCTTTTGAAAACCCAGAATGTGATGCATTTGATGTCTTTGTTAATGAGTTTCTTTGTGTTGGAAAAGGTACCATCTTTGCTCCAACTTATGAATGTTACAATTTAAGTTCTTTGTTACAATACAACAATCTACCTGTGTTGTGTCATAACAATGTGCAGGTTGCCCATATTCGTGTGTAGACAGAGCTCCCCATGCCTTCACTTTTGCTTCGTCCACCGGGACAGCCCGGGCGACTTCTCAAGGTTCGTTCGAGTGATTATATGCAATTATAATTTCAGTATTTCGAGTGACATATGAAATGTGTATTATTCCTACAACGATTTTATCTTTCGATAAACTAGGACACGGTGAAGATTATCAGGTTCAAATTGCAGTTGGCCAATGCCCCAGAAGCTGTATACATTATGTAACACCTTTGCAAAGAATCATTCTGGTAGAGCTACTTGACAGGTATAAAAATATTCCAACTTAGATTCATGTTCGAAACTATGGTGTCCCACGTTGGTCGGGGAGGAGAACAAACCACCATTTATAAGAGTATGGAAACCTTCCCCTAGAAGACGCGTTTTAAAGCCTTGAGGGGAAGCCCAAAGAGGACAATATCTGCTAGCGGTGGATCTGGGTCGTTACTAATGGTATCAGAGCCAGACACTGGACGATGTGCCAGCCTTCTCGCTGTTCCTTGAAGGGGTAGACAAGAGGCGGTGTGCTAGTAAGGACGCTGGGCCCCAAAGGGGGTGGATTTGGGGACGGTCCCACATCCATTGGAGAAAGGAAAGAGTGCCGGCGAGGACGCTGGGTCCTGAAGGGGAGTAGATTGTGATGTCCCACATTGGTTGGGGAGGAGAACAAACCACCATTTATAAGGGTGTGGAAACTAGCAGACAAGCCTTGAGGGGAAGCCCGAGAAGGAAAAGCCCAAAGAGAATAATATCCACTAGTGGATCTGGGTCGTTACAGAAACCTTACGACTAGAAATCTCAACCCCTCTAAACTTGAATTTTATACTGAATTTATATTATTTATTAAAGGTTCTTACCTTTACTTTATTAGTTTAATTAGAAAGTAGCCTAGTTCGGCTGGGCGAAGCTCCGAATGGAAAGAGTACTCTCGTTAAAATAGGTTCTTACCACATAGCTTTTTGGTGACTTAAGTTCATCTTTGTTTGTGTAGTGCCTTGGATGTGCCATATGATAATTCAGCAGAGGCAGATTTACTATATTCTCTTATAGTGAAATCAAAATTCGAGAATAATCGATACAGGAAGCCGAAGAGGGAGCCAAAGACGTCGACCGAACGAGTCGATTGGTATTGAAATCTTGATTTTACCATCTCGTGTTGAACTGAATCAAGAAGAAACCAATGCAGGTGACAAAATATCTTCTAAGTTCTCTAAAGAAACATATGTGTTAGAGTTCGGTGGGTTAGTTTATTACCCATCAAATGGGATCATTTCGATTCTTTAAATGTTTTAGATTAAGATTTGTATATATCTTAGGGGATCGTTTTGTAATAGAATTATTATTACTATTTTATAATT

mRNA sequence

AAATCAACTTCCCGCGAACGCCGACAATGGAGGCGCTGTATCTCTCCACTTCAGCACCAACCGTTCTTCCATTTCGGAAAACCGTTGGCTCTCGAACCATTTCAAAATCCTCGATTCTCTTCCCTTCTTCATCAAAGTCTCGATTCGCAACGATTTGCAGGCAAAGAATTGATGGAGAAGATACGCCTCTCTCCAATGCTATGGATGCTTACAGATTGTTCGGCGTCGACCCGACCTGCTCCGAATCCGAGCTCAAAGCCGCTTTTCGAGCTAAAGTGAAGCAATTCCATCCAGACGTGAATAGAGATGGAAATTATTCCGATTCTATGATTCGTCGTGTAATTCAGGCGTACGAGATGTTGTCAAGTTGCAGCCGAACCGAGTTCATTGAGAGGTTGCCCATATTCGTGTGTAGACAGAGCTCCCCATGCCTTCACTTTTGCTTCGTCCACCGGGACAGCCCGGGCGACTTCTCAAGTGCCTTGGATGTGCCATATGATAATTCAGCAGAGGCAGATTTACTATATTCTCTTATAGTGAAATCAAAATTCGAGAATAATCGATACAGGAAGCCGAAGAGGGAGCCAAAGACGTCGACCGAACGAGTCGATTGGTATTGAAATCTTGATTTTACCATCTCGTGTTGAACTGAATCAAGAAGAAACCAATGCAGGTGACAAAATATCTTCTAAGTTCTCTAAAGAAACATATGTGTTAGAGTTCGGTGGGTTAGTTTATTACCCATCAAATGGGATCATTTCGATTCTTTAAATGTTTTAGATTAAGATTTGTATATATCTTAGGGGATCGTTTTGTAATAGAATTATTATTACTATTTTATAATT

Coding sequence (CDS)

ATGGAGGCGCTGTATCTCTCCACTTCAGCACCAACCGTTCTTCCATTTCGGAAAACCGTTGGCTCTCGAACCATTTCAAAATCCTCGATTCTCTTCCCTTCTTCATCAAAGTCTCGATTCGCAACGATTTGCAGGCAAAGAATTGATGGAGAAGATACGCCTCTCTCCAATGCTATGGATGCTTACAGATTGTTCGGCGTCGACCCGACCTGCTCCGAATCCGAGCTCAAAGCCGCTTTTCGAGCTAAAGTGAAGCAATTCCATCCAGACGTGAATAGAGATGGAAATTATTCCGATTCTATGATTCGTCGTGTAATTCAGGCGTACGAGATGTTGTCAAGTTGCAGCCGAACCGAGTTCATTGAGAGGTTGCCCATATTCGTGTGTAGACAGAGCTCCCCATGCCTTCACTTTTGCTTCGTCCACCGGGACAGCCCGGGCGACTTCTCAAGTGCCTTGGATGTGCCATATGATAATTCAGCAGAGGCAGATTTACTATATTCTCTTATAGTGAAATCAAAATTCGAGAATAATCGATACAGGAAGCCGAAGAGGGAGCCAAAGACGTCGACCGAACGAGTCGATTGGTATTGA

Protein sequence

MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEFIERLPIFVCRQSSPCLHFCFVHRDSPGDFSSALDVPYDNSAEADLLYSLIVKSKFENNRYRKPKREPKTSTERVDWY

Homology

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BLAST of Carg17543 vs. NCBI nr
Match: XP_023537184.1 (uncharacterized protein LOC111798340 [Cucurbita pepo subsp. pepo] >XP_023537185.1 uncharacterized protein LOC111798340 [Cucurbita pepo subsp. pepo] >XP_023537187.1 uncharacterized protein LOC111798340 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 310.5 bits (794), Expect = 4.2e-81
Identity = 178/261 (68.20%), Postives = 182/261 (69.73%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60
           MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD
Sbjct: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60

Query: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120
           AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF
Sbjct: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120

Query: 121 IER-------------LPIFV-------------CRQSSP-------------------- 180
           IER               +FV             C   +P                    
Sbjct: 121 IERECLDPFENPECDAFDVFVNEFLCVGRGCPYSCVDRAPHVFTFASSTGTARATSQGHG 180

Query: 181 -----------CLHFCFVHRDSPGD-------FSSALDVPYDNSAEADLLYSLIVKSKFE 198
                      C   C +H  +P           SALDVPYDNSAEADLLYSLIVKSKFE
Sbjct: 181 EDYQVQIAVGQCPRSC-IHYVTPLQRIILEELLDSALDVPYDNSAEADLLYSLIVKSKFE 240

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BLAST of Carg17543 vs. NCBI nr
Match: XP_022937591.1 (uncharacterized protein LOC111443953 [Cucurbita moschata] >XP_022937592.1 uncharacterized protein LOC111443953 [Cucurbita moschata] >XP_022937593.1 uncharacterized protein LOC111443953 [Cucurbita moschata])

HSP 1 Score: 307.8 bits (787), Expect = 2.7e-80
Identity = 177/261 (67.82%), Postives = 181/261 (69.35%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60
           MEALYLSTSAPTVLPFRKTVGSRTISKSSI FPSSSKSRFATICRQRIDGEDTPLSNAMD
Sbjct: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSIPFPSSSKSRFATICRQRIDGEDTPLSNAMD 60

Query: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120
           AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF
Sbjct: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120

Query: 121 IER-------------LPIFV-------------CRQSSP-------------------- 180
           IER               +FV             C   +P                    
Sbjct: 121 IERECLDPFENPECDAFDVFVNEFLCVGKGCPYSCVDRAPHAFTFASSTGTARATSQGHG 180

Query: 181 -----------CLHFCFVHRDSPGD-------FSSALDVPYDNSAEADLLYSLIVKSKFE 198
                      C   C +H  +P           SALDVPYDNSAEADLLYSLIVKSKFE
Sbjct: 181 EDYQVQIAVGQCPRSC-IHYVTPLQRIILEELLDSALDVPYDNSAEADLLYSLIVKSKFE 240

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BLAST of Carg17543 vs. NCBI nr
Match: XP_022965891.1 (uncharacterized protein LOC111465640 [Cucurbita maxima])

HSP 1 Score: 301.2 bits (770), Expect = 2.6e-78
Identity = 172/261 (65.90%), Postives = 180/261 (68.97%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60
           MEALY STSAP VLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD
Sbjct: 1   MEALYFSTSAPPVLPFRKTVGSRTISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMD 60

Query: 61  AYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120
           AY+LFGVDPTCSESELKAAFRAKVK+FHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF
Sbjct: 61  AYKLFGVDPTCSESELKAAFRAKVKEFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEF 120

Query: 121 IER-------------LPIFV-------------CRQSSP-------------------- 180
           IER               +FV             C   +P                    
Sbjct: 121 IERECLDPFENPECDAFDVFVNEFLCVGKGCPYSCVGRAPHVFTFASSTGTARATSQGHG 180

Query: 181 -----------CLHFCFVHRDSPGD-------FSSALDVPYDNSAEADLLYSLIVKSKFE 198
                      C   C +H  +P           SALDVPYDNSAE+DLLYSLIVKS+FE
Sbjct: 181 EDYQVQIAVGQCPRSC-IHYVTPLQRIILEELLDSALDVPYDNSAESDLLYSLIVKSRFE 240

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BLAST of Carg17543 vs. NCBI nr
Match: KGN60912.1 (hypothetical protein Csa_2G022810 [Cucumis sativus])

HSP 1 Score: 244.2 bits (622), Expect = 3.7e-61
Identity = 154/268 (57.46%), Postives = 164/268 (61.19%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGS-------RTISKSSILFPSSSKSRFATICRQRIDGEDT 60
           MEAL  ST   TVLPFRKTVGS        +ISKSSILFPSSS SRF  ICRQR +GEDT
Sbjct: 1   MEALCFST---TVLPFRKTVGSFNDTSNFPSISKSSILFPSSSTSRFTPICRQRTEGEDT 60

Query: 61  PLSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLS 120
            LS  M AYRL G+DPTCSESELKAAFRAKVKQFHPDVNR+GN SDSMIRRVIQAYEMLS
Sbjct: 61  LLSGPMAAYRLLGLDPTCSESELKAAFRAKVKQFHPDVNRNGNDSDSMIRRVIQAYEMLS 120

Query: 121 SCSRTEFIER-------------LPIFV-------------CRQSSP------------- 180
           S SRTEFIER               +FV             C   +P             
Sbjct: 121 SYSRTEFIERECLDPFENPECEAFDVFVNEFLCVGKGCPYSCVDRAPHVFTFASSTGTAR 180

Query: 181 ------------------CLHFCFVHRDSPGD-------FSSALDVPYDNSAEADLLYSL 198
                             C   C +H  +P           SALDVPYDNSAEADLLYSL
Sbjct: 181 AMSQGHSEDYQLQMAVGQCPRSC-IHYVTPLQRIILEELLDSALDVPYDNSAEADLLYSL 240

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BLAST of Carg17543 vs. NCBI nr
Match: XP_022150755.1 (pentatricopeptide repeat-containing protein At3g05340 [Momordica charantia])

HSP 1 Score: 233.8 bits (595), Expect = 5.0e-58
Identity = 146/270 (54.07%), Postives = 162/270 (60.00%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGS-------RTISKSSILFPSSSK--SRFATICRQRIDGE 60
           M AL    S PT+LPFRKTVGS        + SK SILFPSSS   SRFATICRQR +GE
Sbjct: 684 MAALCFYASTPTILPFRKTVGSPNDSSNFPSKSKPSILFPSSSSSASRFATICRQRSEGE 743

Query: 61  DTPLSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEM 120
           D PLS A+ AYR+ GVDPTCS+SELKAAFRAKVKQFHPDVN +GN SDSMIRRVIQAYEM
Sbjct: 744 DAPLSTALAAYRVLGVDPTCSQSELKAAFRAKVKQFHPDVNGNGNASDSMIRRVIQAYEM 803

Query: 121 LSSCSRTEFIER-------------LPIFV-------------CRQSSP----------- 180
           LSS SRTEFIER               +FV             C  ++P           
Sbjct: 804 LSSYSRTEFIERECLDPFENPECQAFDVFVNEFLCVGKGCPYSCVDTAPHVFTFASSTGT 863

Query: 181 --------------------CLHFCFVHRDSPGD-------FSSALDVPYDNSAEADLLY 198
                               C   C +H  +P           SAL+VPYDNS EADLLY
Sbjct: 864 ARATSQGHGEDYQVLIAAGQCPRSC-IHYVTPLQRIILEELLDSALNVPYDNSVEADLLY 923

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BLAST of Carg17543 vs. TAIR10
Match: AT3G05345.1 (Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 140.2 bits (352), Expect = 1.4e-33
Identity = 92/226 (40.71%), Postives = 121/226 (53.54%), Query Frame = 0


Query: 35  SSKSRFATICRQRIDGEDTPLSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRD 94
           S+  RF   CR +    + PLS++   Y + GV+P+CS SELKAAFRAKVKQ+HPDVN+D
Sbjct: 22  STSQRFIPSCRGK--NREDPLSSS-SPYSILGVEPSCSSSELKAAFRAKVKQYHPDVNKD 81

Query: 95  GNYSDSMIRRVIQAYEMLSSCSRTEFI-------------ERLPIFV----C---RQSSP 154
           G+ SD MIRR+IQAYEML++ SR+E I             E L +FV    C   R S P
Sbjct: 82  GSNSDIMIRRIIQAYEMLTNYSRSEIIEGECLDPFDHPECEALDVFVNEVLCVGKRCSYP 141

Query: 155 CLHFC-----------------------------------FVHRDSPGD-------FSSA 198
           C                                        +H  +P           S 
Sbjct: 142 CFETASHVFSCDSSGTARAMSQGHGEDYRVQSAVNQCPRNCIHYVTPSQRIILEELLDSV 201

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BLAST of Carg17543 vs. TAIR10
Match: AT4G39960.1 (Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 49.3 bits (116), Expect = 3.2e-06
Identity = 21/64 (32.81%), Postives = 36/64 (56.25%), Query Frame = 0


Query: 60  DAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTE 119
           D Y + GV    +++E+K+A+R   + +HPDVN+D    D   + +  AYE+LS   +  
Sbjct: 85  DFYSVLGVSKNATKAEIKSAYRKLARSYHPDVNKDAGAEDK-FKEISNAYEILSDDEKRS 144

Query: 120 FIER 124
             +R
Sbjct: 145 LYDR 147

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BLAST of Carg17543 vs. TAIR10
Match: AT2G22360.1 (DNAJ heat shock family protein)

HSP 1 Score: 47.8 bits (112), Expect = 9.2e-06
Identity = 20/64 (31.25%), Postives = 37/64 (57.81%), Query Frame = 0


Query: 60  DAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTE 119
           D Y + GV    +++E+K+A+R   + +HPDVN+D   ++   + +  AYE+LS   +  
Sbjct: 86  DYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPG-AEEKFKEISNAYEVLSDDEKKS 145

Query: 120 FIER 124
             +R
Sbjct: 146 LYDR 148

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BLAST of Carg17543 vs. TAIR10
Match: AT1G80030.1 (Molecular chaperone Hsp40/DnaJ family protein)

HSP 1 Score: 46.2 bits (108), Expect = 2.7e-05
Identity = 20/54 (37.04%), Postives = 33/54 (61.11%), Query Frame = 0


Query: 60  DAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLS 114
           D Y   GV  + +  E+KAA+R   +Q+HPDVN++   ++   + +  AYE+LS
Sbjct: 75  DYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEPGATEK-FKEISAAYEVLS 127

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BLAST of Carg17543 vs. TAIR10
Match: AT4G21180.1 (DnaJ / Sec63 Brl domains-containing protein)

HSP 1 Score: 45.1 bits (105), Expect = 6.0e-05
Identity = 20/68 (29.41%), Postives = 36/68 (52.94%), Query Frame = 0


Query: 56  SNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSC 115
           S   + + + G++P  S+SE+K A+R    Q+HPD N D   +   +  + +AY+ L+  
Sbjct: 95  SQLFEPFGILGLEPGASDSEIKKAYRRLSIQYHPDKNPDPEANKYFVESIAKAYQALTDP 154

Query: 116 SRTEFIER 124
              E  E+
Sbjct: 155 LSRENFEK 162

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BLAST of Carg17543 vs. Swiss-Prot
Match: sp|B8I304|DNAJ_CLOCE (Chaperone protein DnaJ OS=Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10) OX=394503 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.8e-06
Identity = 23/63 (36.51%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 55  LSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSS 114
           +++  D Y + GVD   S++ELK A+R   K++HPDVN     +++  + V +AYE+LS 
Sbjct: 1   MADKRDYYEVLGVDKNASDAELKKAYRNLAKKYHPDVNPGDTTAEAKFKEVNEAYEILSD 60

Query: 115 CSR 118
             +
Sbjct: 61  SQK 63

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BLAST of Carg17543 vs. Swiss-Prot
Match: sp|A3DF24|DNAJ_CLOTH (Chaperone protein DnaJ OS=Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 6.8e-06
Identity = 21/59 (35.59%), Postives = 37/59 (62.71%), Query Frame = 0


Query: 55  LSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLS 114
           ++   D Y + GVD   S++E+K A+R   KQ+HPD+N     +++  + + +AYE+LS
Sbjct: 1   MAGKRDYYEILGVDRGASDAEIKKAYRKLAKQYHPDMNPGDKAAEAKFKEINEAYEVLS 59

[Close]

BLAST of Carg17543 vs. Swiss-Prot
Match: sp|B1ZUS0|DNAJ_OPITP (Chaperone protein DnaJ OS=Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1) OX=452637 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 6.8e-06
Identity = 24/64 (37.50%), Postives = 34/64 (53.12%), Query Frame = 0


Query: 60  DAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTE 119
           D Y L GV    SE ELK A+R K  Q+HPD N     ++ M +++  AYE+L    +  
Sbjct: 5   DYYELLGVQKGASEEELKKAYRKKAVQYHPDKNPGNKEAEEMFKKISHAYEVLKDPEKRA 64

Query: 120 FIER 124
             +R
Sbjct: 65  AYDR 68

[Close]

BLAST of Carg17543 vs. Swiss-Prot
Match: sp|A3PNM0|DNAJ_RHOS1 (Chaperone protein DnaJ OS=Rhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9) OX=349101 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 6.8e-06
Identity = 23/64 (35.94%), Postives = 35/64 (54.69%), Query Frame = 0


Query: 60  DAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTE 119
           D Y + GV  T S  ELK A+R K K+ HPD N D   +++  + V +AY++L    +  
Sbjct: 5   DYYEVLGVSRTASADELKKAYRTKAKELHPDRNADNPQAEAQFKEVNEAYDVLRDADKKA 64

Query: 120 FIER 124
             +R
Sbjct: 65  AYDR 68

[Close]

BLAST of Carg17543 vs. Swiss-Prot
Match: sp|Q3IYM8|DNAJ_RHOS4 (Chaperone protein DnaJ OS=Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) OX=272943 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 6.8e-06
Identity = 23/64 (35.94%), Postives = 35/64 (54.69%), Query Frame = 0


Query: 60  DAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTE 119
           D Y + GV  T S  ELK A+R K K+ HPD N D   +++  + V +AY++L    +  
Sbjct: 5   DYYEVLGVSRTASADELKKAYRTKAKELHPDRNADNPQAEAQFKEVNEAYDVLRDADKKA 64

Query: 120 FIER 124
             +R
Sbjct: 65  AYDR 68

[Close]

BLAST of Carg17543 vs. TrEMBL
Match: tr|A0A0A0LJM6|A0A0A0LJM6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G022810 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.5e-61
Identity = 154/268 (57.46%), Postives = 164/268 (61.19%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVGS-------RTISKSSILFPSSSKSRFATICRQRIDGEDT 60
           MEAL  ST   TVLPFRKTVGS        +ISKSSILFPSSS SRF  ICRQR +GEDT
Sbjct: 1   MEALCFST---TVLPFRKTVGSFNDTSNFPSISKSSILFPSSSTSRFTPICRQRTEGEDT 60

Query: 61  PLSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLS 120
            LS  M AYRL G+DPTCSESELKAAFRAKVKQFHPDVNR+GN SDSMIRRVIQAYEMLS
Sbjct: 61  LLSGPMAAYRLLGLDPTCSESELKAAFRAKVKQFHPDVNRNGNDSDSMIRRVIQAYEMLS 120

Query: 121 SCSRTEFIER-------------LPIFV-------------CRQSSP------------- 180
           S SRTEFIER               +FV             C   +P             
Sbjct: 121 SYSRTEFIERECLDPFENPECEAFDVFVNEFLCVGKGCPYSCVDRAPHVFTFASSTGTAR 180

Query: 181 ------------------CLHFCFVHRDSPGD-------FSSALDVPYDNSAEADLLYSL 198
                             C   C +H  +P           SALDVPYDNSAEADLLYSL
Sbjct: 181 AMSQGHSEDYQLQMAVGQCPRSC-IHYVTPLQRIILEELLDSALDVPYDNSAEADLLYSL 240

[Close]

BLAST of Carg17543 vs. TrEMBL
Match: tr|A0A1S4E0D4|A0A1S4E0D4_CUCME (pentatricopeptide repeat-containing protein At3g05340 OS=Cucumis melo OX=3656 GN=LOC103494955 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.6e-57
Identity = 139/238 (58.40%), Postives = 148/238 (62.18%), Query Frame = 0


Query: 24  TISKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMDAYRLFGVDPTCSESELKAAFRAK 83
           +ISKSSILF SSS SRF TICRQRI+GEDTPLS  M AYRL G+DPTCSESELKAAFRAK
Sbjct: 666 SISKSSILFSSSSTSRFTTICRQRIEGEDTPLSGPMAAYRLLGLDPTCSESELKAAFRAK 725

Query: 84  VKQFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEFIER-------------LPIFV-- 143
           VKQFHPDV R+GN SDSMIRRVIQAYEMLSS SRTEFIER               +FV  
Sbjct: 726 VKQFHPDVTRNGNDSDSMIRRVIQAYEMLSSYSRTEFIERECLDPFENPECEAFDVFVNE 785

Query: 144 -----------CRQSSP-------------------------------CLHFCFVHRDSP 198
                      C   +P                               C   C +H  +P
Sbjct: 786 FLCVGKGCPYSCVDRAPHVFTFASSTGTARATSQGHSEDYQLQMAVGQCPRSC-IHYVTP 845

[Close]

BLAST of Carg17543 vs. TrEMBL
Match: tr|B9RF61|B9RF61_RICCO (Pentatricopeptide repeat-containing protein, putative OS=Ricinus communis OX=3988 GN=RCOM_1431500 PE=4 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 4.1e-40
Identity = 105/236 (44.49%), Postives = 134/236 (56.78%), Query Frame = 0


Query: 26  SKSSILFPSSSKSRFATICRQRIDGEDTPLSNAMDAYRLFGVDPTCSESELKAAFRAKVK 85
           S++ + FPS S S+F   C +RI G+DTPLS++  A+ + GVDP CS +ELKAAFRAKVK
Sbjct: 503 SRNPLRFPSHSNSKFTVNCSERI-GDDTPLSSSSAAHMVLGVDPGCSAAELKAAFRAKVK 562

Query: 86  QFHPDVNRDGNYSDSMIRRVIQAYEMLSSCSRTEFI-------------ERLPIFV---- 145
           QFHPDVNRD  + D+MIRRVIQAYE+LS+ SR+E I             E   IFV    
Sbjct: 563 QFHPDVNRDRKFPDTMIRRVIQAYEILSNFSRSEIIESECLDPFDNPECEAFDIFVNEVL 622

Query: 146 ---------CRQSSP-------------------------------CLHFCFVHRDSPGD 198
                    C Q++P                               C   C +H  +P  
Sbjct: 623 CAGKGCPYSCVQTAPHAFAYASSTGTARAISQGHGEDYKVQLAVGQCPRSC-IHYVTPSQ 682

[Close]

BLAST of Carg17543 vs. TrEMBL
Match: tr|A0A2I4GI41|A0A2I4GI41_9ROSI (uncharacterized protein LOC109008071 isoform X2 OS=Juglans regia OX=51240 GN=LOC109008071 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 9.1e-40
Identity = 118/266 (44.36%), Postives = 146/266 (54.89%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTV----GSRTIS--KSSILFPSSSKSRFATICRQRIDGEDTP 60
           ME L L +S P++LPFRK V    G    S  ++  LF SS  S   T CR+RI G D P
Sbjct: 1   MECLLLCSSTPSILPFRKAVTFPNGHHFPSNFRNPELFSSSLSSLNYTNCRERI-GVDAP 60

Query: 61  LSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEMLSS 120
           LS+   AY + GV+P CS +ELKAAFRAKVKQFHPDVNRDG  SD+MIRRVIQAYEMLS+
Sbjct: 61  LSST-SAYVVLGVEPDCSAAELKAAFRAKVKQFHPDVNRDGPDSDTMIRRVIQAYEMLSN 120

Query: 121 CSRTEFIER-------------LPIFV-------------CRQSSP-------------- 180
            SR+E IER               +FV             C + +P              
Sbjct: 121 YSRSEIIERECLDPFENPECEAFDLFVNEVLCLGKGCPYSCVKGAPHAFTYASTGTARVS 180

Query: 181 ----------------CLHFCFVHRDSPGD-------FSSALDVPYDNSAEADLLYSLIV 198
                           C   C ++  +P           S L+ PYD SAEA+LLYSL+ 
Sbjct: 181 SQGHGDDYQVQMAVGQCPRSC-IYYVTPSQRIILEELLDSILNKPYDTSAEAELLYSLMA 240

[Close]

BLAST of Carg17543 vs. TrEMBL
Match: tr|A0A2C9VBH2|A0A2C9VBH2_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_09G167200 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 9.1e-40
Identity = 116/269 (43.12%), Postives = 147/269 (54.65%), Query Frame = 0


Query: 1   MEALYLSTSAPTVLPFRKTVG--------SRTISKSSILFPSSSKSRFATICRQRIDGED 60
           ME+L   +S P ++ F+K V         S   S++ +LFP SS S+F   C +R   +D
Sbjct: 1   MESLCFYSSIPKIVQFQKFVTNPNCHNSISNFNSRNPLLFPWSSNSKFTVHCTER-TRDD 60

Query: 61  TPLSNAMDAYRLFGVDPTCSESELKAAFRAKVKQFHPDVNRDGNYSDSMIRRVIQAYEML 120
           TPLS++  AY + GV   CS +ELKAAFRAKVKQFHPDVNRDG+ SD+MIRRVIQAY+ML
Sbjct: 61  TPLSSS-SAYAVLGVQLGCSPAELKAAFRAKVKQFHPDVNRDGDDSDTMIRRVIQAYQML 120

Query: 121 SSCSRTEFI-------------ERLPIFV-------------CRQSSP------------ 180
           S+CSR E I             E L IFV             C +S+P            
Sbjct: 121 SNCSRLEIIEGECLDPFDKPECEALDIFVNEVLCVGKGCPYSCVRSAPHAFAYDSSTGTA 180

Query: 181 -------------------CLHFCFVHRDSPGD-------FSSALDVPYDNSAEADLLYS 198
                              C   C +H  +P           S L+ PYD SAEADLLYS
Sbjct: 181 RATSQGHGEDYQVQLAVGQCPRSC-IHYVTPSQRIILEELLDSILNAPYDCSAEADLLYS 240

The following BLAST results are available for this feature:

BLAST of Carg17543 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023537184.1	4.2e-81	68.20	uncharacterized protein LOC111798340 [Cucurbita pepo subsp. pepo] >XP_023537185....	[more]
XP_022937591.1	2.7e-80	67.82	uncharacterized protein LOC111443953 [Cucurbita moschata] >XP_022937592.1 unchar...	[more]
XP_022965891.1	2.6e-78	65.90	uncharacterized protein LOC111465640 [Cucurbita maxima]	[more]
KGN60912.1	3.7e-61	57.46	hypothetical protein Csa_2G022810 [Cucumis sativus]	[more]
XP_022150755.1	5.0e-58	54.07	pentatricopeptide repeat-containing protein At3g05340 [Momordica charantia]	[more]

BLAST of Carg17543 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G05345.1	1.4e-33	40.71	Chaperone DnaJ-domain superfamily protein	[more]
AT4G39960.1	3.2e-06	32.81	Molecular chaperone Hsp40/DnaJ family protein	[more]
AT2G22360.1	9.2e-06	31.25	DNAJ heat shock family protein	[more]
AT1G80030.1	2.7e-05	37.04	Molecular chaperone Hsp40/DnaJ family protein	[more]
AT4G21180.1	6.0e-05	29.41	DnaJ / Sec63 Brl domains-containing protein	[more]

BLAST of Carg17543 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|B8I304\|DNAJ_CLOCE	1.8e-06	36.51	Chaperone protein DnaJ OS=Clostridium cellulolyticum (strain ATCC 35319 / DSM 58...	[more]
sp\|A3DF24\|DNAJ_CLOTH	6.8e-06	35.59	Chaperone protein DnaJ OS=Clostridium thermocellum (strain ATCC 27405 / DSM 1237...	[more]
sp\|B1ZUS0\|DNAJ_OPITP	6.8e-06	37.50	Chaperone protein DnaJ OS=Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1...	[more]
sp\|A3PNM0\|DNAJ_RHOS1	6.8e-06	35.94	Chaperone protein DnaJ OS=Rhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9...	[more]
sp\|Q3IYM8\|DNAJ_RHOS4	6.8e-06	35.94	Chaperone protein DnaJ OS=Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / N...	[more]

BLAST of Carg17543 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LJM6\|A0A0A0LJM6_CUCSA	2.5e-61	57.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G022810 PE=4 SV=1	[more]
tr\|A0A1S4E0D4\|A0A1S4E0D4_CUCME	1.6e-57	58.40	pentatricopeptide repeat-containing protein At3g05340 OS=Cucumis melo OX=3656 GN...	[more]
tr\|B9RF61\|B9RF61_RICCO	4.1e-40	44.49	Pentatricopeptide repeat-containing protein, putative OS=Ricinus communis OX=398...	[more]
tr\|A0A2I4GI41\|A0A2I4GI41_9ROSI	9.1e-40	44.36	uncharacterized protein LOC109008071 isoform X2 OS=Juglans regia OX=51240 GN=LOC...	[more]
tr\|A0A2C9VBH2\|A0A2C9VBH2_MANES	9.1e-40	43.12	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_09G167200 PE=4 SV=...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR036869	J_dom_sf
IPR001623	DnaJ_domain

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg17543-RA	Carg17543-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 80..95 score: 35.33 coord: 97..117 score: 22.9 coord: 62..80 score: 34.98
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 59..117 e-value: 2.5E-12 score: 57.0
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 60..114 e-value: 1.8E-10 score: 40.7
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 60..125 score: 12.552
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 60..113 e-value: 2.52122E-13 score: 60.6369
IPR036869	Chaperone J-domain superfamily	GENE3D	G3DSA:1.10.287.110		coord: 46..124 e-value: 4.3E-13 score: 51.2
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	SSF46565	Chaperone J-domain	coord: 56..123
None	No IPR available	PANTHER	PTHR24074:SF12	DNAJ HOMOLOG SUBFAMILY C MEMBER 7 HOMOLOG	coord: 54..113
None	No IPR available	PANTHER	PTHR24074	FAMILY NOT NAMED	coord: 54..113

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg17543	Cla019892	Watermelon (97103) v1	carwmB0714
Carg17543	Csa2G022810	Cucumber (Chinese Long) v2	carcuB0735
Carg17543	MELO3C004256	Melon (DHL92) v3.5.1	carmeB0711
Carg17543	ClCG02G015790	Watermelon (Charleston Gray)	carwcgB0692
Carg17543	CSPI02G03980	Wild cucumber (PI 183967)	carcpiB0751
Carg17543	Cucsa.091430	Cucumber (Gy14) v1	carcgyB0803
Carg17543	CmoCh12G010850	Cucurbita moschata (Rifu)	carcmoB0973
Carg17543	Cp4.1LG07g09030	Cucurbita pepo (Zucchini)	carcpeB1006
Carg17543	CsGy2G004080	Cucumber (Gy14) v2	carcgybB0713
Carg17543	MELO3C004256.2	Melon (DHL92) v3.6.1	carmedB0724
Carg17543	CsaV3_2G005310	Cucumber (Chinese Long) v3	carcucB0754
Carg17543	Cla97C02G041520	Watermelon (97103) v2	carwmbB0746

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg17543	Cucurbita maxima (Rimu)	carcmaB1000
Carg17543	Cucurbita maxima (Rimu)	carcmaB1001
Carg17543	Cucurbita moschata (Rifu)	carcmoB0974
Carg17543	Cucurbita pepo (Zucchini)	carcpeB1005
Carg17543	Bottle gourd (USVL1VR-Ls)	carlsiB654