Carg16984 (gene) Silver-seed gourd

NameCarg16984
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionCystathionine gamma synthase
LocationCucurbita_argyrosperma_scaffold_061 : 746098 .. 750243 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTCAGCTTCCTCCTTCCCCTGCGATTCGCAGCTCTCCGACCCACTACTTTTCCGATCCATTTGCTCTTTCTTTCTCTTCCGCCATGGCTGTTTCTTCTCACGCTGCACTCATTGGTTCGGCCTCCTTCGAGGGCCTCTCCGATCCTAATTCCGGCGTTGCAGCTTGTCTTCGGACGCACAGCTATGCCCGATTTGCCCAACCCAAATCGGTGAAAGGGACGTTGGCGTCTCACGCCTTATCTTCTTCCCGGATTTTTAAGTTCCCGCCGAATTTCCTCAGGCAACTCAGTAACAAGGCGCGAAGGAACTGCAGCAATATTGGCGTTGCACAGGTTGTTGCGGCTTCGTGGTCGAATAACTCCCCTCCTTCTTCCTCGGCGGCGGCGGCGGCGGCTACTTCCGTCGACGCCACTGCCGCTGCCTCAGTGCCTGCCGCGTCTGATGCGGTGGCACTGGAAGGTTGCGTTGGGAATGAGAGTTTGAAGGTGGAGGAGGATTTAGGTGATTCCAGAACCTCTGTCTTCCATTCCGATGGGAGTGTTGCTATCCACGCTGGTAAGGTTTAGTTAGAAATTTGGCTTTGTCTTTGATTCTTTGAGATTCTTACAATAAACTTTTTCTTTCTTTTCCTTTTTGTTGTTTTTAGGAATATTTTTATTATTTACATATAATTGAATGTTTCGGTGCGGATTTCAGGCGAACGATTTGGTCGTGGTAGAGTTGACGACGCTATTACGACGCCGGTGGTTAATACTTCCGCTTATTTCTTCAAGAAAACTGCTGATCTCATTGATTTCAAGGTACTTATTAGCTTTCCATGATGATTCAAAACGGTTGACTTCAACTCTGTAGCTTTTATTATCATTTTCTCCCTACTAGTCTAGCATTGTTTTGCTCGGTATACATGCATCAAAATTGAAGAAAAAAAAAAAAAAAACTATATATAAAGATGTCTGGATTCGTTCTTACTCTTTTAGACATTGGATCCTGGCAGGAGAAGCGCGCTGTAAGTTTCGAGTATGGGCGCTATGGAAATCCGACGACAATTGTTGCGGAGGAGAAGATAAGGTTTTGTTTATGAACTAGTTTGTTTCTTTTGTAATTCTTACCAAATAGAATTGAGTGTTGTAAGTTGTTTTTGGTTTTGAATCAGTGCTTTAGAAGGGGCTGAATCTACCCTGATTGTGGCCTCAGGCATGTGTGCTAGCACGATGATGTTTCTGGCGTTGGTTCCTGCTGGAGGGCATATCGTGACGACCACAGATTGCTATAGGAAGACAAGGATTTTTATTGAAACCATTCTTCCCAAAATGGGAATCACGGTATTATTGTACCTTTAGCTTCATTCTGTTTATTTTATTCATCTTCTTTCGGTGGTTTAGTTAATCATTACGCTAAGATTAGTGTTATTTAACATTTTTCTGTCTCATTCAATGATTGGACACCAACTCGATCTGTCTTGCATGAAAATAGTCTTTTCCCTTATGTATGCATTAATTTAACTGTGACAGAATAATTTTGTTTTTATTCGGAGTAATATTTGAACTTGTTTTCTGCTTGGACATTGGCGGAAAAGTAAGTAAACTGCCTTCCCCTGATAAAATTGTGTGTACAGGGTACGAACAAGTTACCTTTAGGTTTCTAGAATTGAGTAAAATAATTAAGCAGCTTTAGTTGATTGGGGGCAAGTCTATCAGGTGCCGTGCCGCAGCCTTAGTTCATACTTTTCCTTTCATCCTTATTAAATATTTTTGTTTTCGCATCGAAATAGAATTCATGAAGAACAAGAATATGAGAAAATGCTAATTAAGGAACATAGAGCAAACAAATTACTAGAATTATAAAGTTTCCTTAAAAATGTAATAGATTCTGTTAACAATCATTCTCTTGTTTATTTGTAGTGATTATATAAGATCTGAATTTTTATTATTGTCGATATTTTTCCAACTATATGGGATTTCTTATCTCTAAAAAAGAAAATTGGATGGATTTGTTTATAAGTTGGTGTTGCTTGATTTGTAGTGGAACTGGTTGGTTGGAAAGCTCTATTCTTAGATCCATTTTTCTTTTATGTTGTAACTGTAACTATAACTTGGAATGCAGGCTACGACCATTGATCCTGCGGATATGAACAGCCTCGAACTAGCTCTGAAAGAAAATAAAGTGACCATTTATCAACCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTATCGTTTTTATGGGTTTTATGTTTGGTGTTAATGGAAATTGATGTTAACCTGTATTGTCCTTCCATGTAGGTTTCTATTTTCTTCACTGAATCCCCAACCAATCCATTTCTGAGATGTGTTGACATTAAACTCGTTTCAGAGCTTTGTCATCAACATGGAGCATTGGTTTGTATAGATGGTACCTTTGCAACAGCATTGAACCAGAAAGCGCTTTCACTCGGGGCCGACTTGGTTATTCACTCTGCTACGAAGTATCTTGGGGGTCATAATGATGTAAGTGATTCTTTTTATTTGAATAATCTGGATTTAAATTATTAATTAATTAAGAGCTTTATGAGCAATTTCCCTTAAGAAGGAATTTACCAAGCATTGTCTTTTAGGTCATTGCTGGTTGTGTAAGCGGTTCTCTGAAACTGGTTTCTGAGATTCGAAACCTTCATCATGTAATAGGTGGCACTTTAAATCCAGTGAGTCCTTCTCTTATCCTATTTAATTCCTGCATCCTTTTTGTTTTTGATTATGGAGTGATGTTCATGCATGATTGAATTCTCCATTGGTTTTCTGCGCTCATTTCGATCCTTTTTTTTTTTTTTCTTTCTTGTCAAGCCTTGCTGTCTAATTAATTCTGATATCTTCATCTTACAGAATGCTGCATACTTACTTATACGAGGCATGAAGACATTGCATCTTCGTGTTCAGCAGCAGAATTCCTCAGCTTTGAGGATGGCAAAGCTTTTAGAGGCTCATCCTAAGGTATGCAATTTAATTTATCAAGCTGCAGTGCTTTATTACAAGGAAAAACACAACTGCATATTGTTTCGTACGTTTAATTTACTACCTAATATTAACCACGGCGGTGCAAGGGAACAATAGTAGTTTAATTATTGATGCTTTTGAGGCATCTGATAGTAGAGTATACGACTCATACACGTGTTGAGAAATTAGCAGATGTAGTAGCATATTCAAACTGAGTTAATCTCATTAATCAAACTTATTCATTAACTGGATTTAATCACGTGGTTGTGAATTCTTTTACCTTATACAATGTGACATTATGCAGATCAAATGTGTTTACTACCCTGGGTTACCAAGTCATCCTGAGCATAATCTTGCCAAAAGACAGATGAGTGGTTTCGGGGGCGTAGTTAGTTTCGAGGTTAGAAAATGACAAAACCCTTTTACTCTGCTAGGTTTAGGCTTAGGTTTAGTTCACGAATTTGTTCATTCTGGTGTGCAGGTTGATGGGGATTTAACGACCACCATAAAATTCATTGATTCATTAAAAATTCCATATATTGCTCCATCATTTGGTGGGTGCGAGAGTATCATAGACCAGCCAGCAATAATGTCATATTGGTAAATATCCAATTCTGAACTTTCGTTAAACACATTTATTTAGTATGTGGGTGGCGGAAGTAATTAAGTTTTTGGTGATGGAAATGAAATAGGGATCTTAACCAAACAGAGAGGCTCAAGTATGGGATAAAGGACAATTTGGTGCGGTTCAGCATTGGAATTGAAGAGTTTGAGGATTTGAAGGCCGATATTCTGCAGGGTCTTGAGGCCATTTAAAGCCTAAGCCTAAGCCTGCCAAAACCAATGAACCTACACCTCTCTTTATATTTAGATATCATCTTTTTTGCTTTGTCAATTGGGATTTGCCAACTCTGGTGGTATTGTCGGCGACGGCGACGTTGATTTGATGGAAATGGTGCCAGGGGCGAAAAAGTGACCCCCCAGGCATTTGTGGTGGTTGGTGTTCTTCTTAACCAATTATTCAAAATCATGGTAGGCCATTGCTTTCTATTGCCTAAAGTTAACAATATCAGGTTCTGAAATTTCGTTCTATGATATAAATCTTAATAATTTATTTTTAAGAATTTCAAACTTGCCAGTTTTAGTAAATACCGAGTACTTGGAGAAATTAA

mRNA sequence

ATTTTCAGCTTCCTCCTTCCCCTGCGATTCGCAGCTCTCCGACCCACTACTTTTCCGATCCATTTGCTCTTTCTTTCTCTTCCGCCATGGCTGTTTCTTCTCACGCTGCACTCATTGGTTCGGCCTCCTTCGAGGGCCTCTCCGATCCTAATTCCGGCGTTGCAGCTTGTCTTCGGACGCACAGCTATGCCCGATTTGCCCAACCCAAATCGGTGAAAGGGACGTTGGCGTCTCACGCCTTATCTTCTTCCCGGATTTTTAAGTTCCCGCCGAATTTCCTCAGGCAACTCAGTAACAAGGCGCGAAGGAACTGCAGCAATATTGGCGTTGCACAGGTTGTTGCGGCTTCGTGGTCGAATAACTCCCCTCCTTCTTCCTCGGCGGCGGCGGCGGCGGCTACTTCCGTCGACGCCACTGCCGCTGCCTCAGTGCCTGCCGCGTCTGATGCGGTGGCACTGGAAGGTTGCGTTGGGAATGAGAGTTTGAAGGTGGAGGAGGATTTAGGTGATTCCAGAACCTCTGTCTTCCATTCCGATGGGAGTGTTGCTATCCACGCTGGCGAACGATTTGGTCGTGGTAGAGTTGACGACGCTATTACGACGCCGGTGGTTAATACTTCCGCTTATTTCTTCAAGAAAACTGCTGATCTCATTGATTTCAAGGAGAAGCGCGCTGTAAGTTTCGAGTATGGGCGCTATGGAAATCCGACGACAATTGTTGCGGAGGAGAAGATAAGTGCTTTAGAAGGGGCTGAATCTACCCTGATTGTGGCCTCAGGCATGTGTGCTAGCACGATGATGTTTCTGGCGTTGGTTCCTGCTGGAGGGCATATCGTGACGACCACAGATTGCTATAGGAAGACAAGGATTTTTATTGAAACCATTCTTCCCAAAATGGGAATCACGGCTACGACCATTGATCCTGCGGATATGAACAGCCTCGAACTAGCTCTGAAAGAAAATAAAGTTTCTATTTTCTTCACTGAATCCCCAACCAATCCATTTCTGAGATGTGTTGACATTAAACTCGTTTCAGAGCTTTGTCATCAACATGGAGCATTGGTTTGTATAGATGGTACCTTTGCAACAGCATTGAACCAGAAAGCGCTTTCACTCGGGGCCGACTTGGTTATTCACTCTGCTACGAAGTATCTTGGGGGTCATAATGATGTCATTGCTGGTTGTGTAAGCGGTTCTCTGAAACTGGTTTCTGAGATTCGAAACCTTCATCATGTAATAGGTGGCACTTTAAATCCAAATGCTGCATACTTACTTATACGAGGCATGAAGACATTGCATCTTCGTGTTCAGCAGCAGAATTCCTCAGCTTTGAGGATGGCAAAGCTTTTAGAGGCTCATCCTAAGATCAAATGTGTTTACTACCCTGGGTTACCAAGTCATCCTGAGCATAATCTTGCCAAAAGACAGATGAGTGGTTTCGGGGGCGTAGTTAGTTTCGAGGTTGATGGGGATTTAACGACCACCATAAAATTCATTGATTCATTAAAAATTCCATATATTGCTCCATCATTTGGTGGGTGCGAGAGTATCATAGACCAGCCAGCAATAATGTCATATTGGGATCTTAACCAAACAGAGAGGCTCAAGTATGGGATAAAGGACAATTTGGTGCGGTTCAGCATTGGAATTGAAGAGTTTGAGGATTTGAAGGCCGATATTCTGCAGGGTCTTGAGGCCATTTAAAGCCTAAGCCTAAGCCTGCCAAAACCAATGAACCTACACCTCTCTTTATATTTAGATATCATCTTTTTTGCTTTGTCAATTGGGATTTGCCAACTCTGGTGGTATTGTCGGCGACGGCGACGTTGATTTGATGGAAATGGTGCCAGGGGCGAAAAAGTGACCCCCCAGGCATTTGTGGTGGTTGGTGTTCTTCTTAACCAATTATTCAAAATCATGGTAGGCCATTGCTTTCTATTGCCTAAAGTTAACAATATCAGGTTCTGAAATTTCGTTCTATGATATAAATCTTAATAATTTATTTTTAAGAATTTCAAACTTGCCAGTTTTAGTAAATACCGAGTACTTGGAGAAATTAA

Coding sequence (CDS)

ATGGCTGTTTCTTCTCACGCTGCACTCATTGGTTCGGCCTCCTTCGAGGGCCTCTCCGATCCTAATTCCGGCGTTGCAGCTTGTCTTCGGACGCACAGCTATGCCCGATTTGCCCAACCCAAATCGGTGAAAGGGACGTTGGCGTCTCACGCCTTATCTTCTTCCCGGATTTTTAAGTTCCCGCCGAATTTCCTCAGGCAACTCAGTAACAAGGCGCGAAGGAACTGCAGCAATATTGGCGTTGCACAGGTTGTTGCGGCTTCGTGGTCGAATAACTCCCCTCCTTCTTCCTCGGCGGCGGCGGCGGCGGCTACTTCCGTCGACGCCACTGCCGCTGCCTCAGTGCCTGCCGCGTCTGATGCGGTGGCACTGGAAGGTTGCGTTGGGAATGAGAGTTTGAAGGTGGAGGAGGATTTAGGTGATTCCAGAACCTCTGTCTTCCATTCCGATGGGAGTGTTGCTATCCACGCTGGCGAACGATTTGGTCGTGGTAGAGTTGACGACGCTATTACGACGCCGGTGGTTAATACTTCCGCTTATTTCTTCAAGAAAACTGCTGATCTCATTGATTTCAAGGAGAAGCGCGCTGTAAGTTTCGAGTATGGGCGCTATGGAAATCCGACGACAATTGTTGCGGAGGAGAAGATAAGTGCTTTAGAAGGGGCTGAATCTACCCTGATTGTGGCCTCAGGCATGTGTGCTAGCACGATGATGTTTCTGGCGTTGGTTCCTGCTGGAGGGCATATCGTGACGACCACAGATTGCTATAGGAAGACAAGGATTTTTATTGAAACCATTCTTCCCAAAATGGGAATCACGGCTACGACCATTGATCCTGCGGATATGAACAGCCTCGAACTAGCTCTGAAAGAAAATAAAGTTTCTATTTTCTTCACTGAATCCCCAACCAATCCATTTCTGAGATGTGTTGACATTAAACTCGTTTCAGAGCTTTGTCATCAACATGGAGCATTGGTTTGTATAGATGGTACCTTTGCAACAGCATTGAACCAGAAAGCGCTTTCACTCGGGGCCGACTTGGTTATTCACTCTGCTACGAAGTATCTTGGGGGTCATAATGATGTCATTGCTGGTTGTGTAAGCGGTTCTCTGAAACTGGTTTCTGAGATTCGAAACCTTCATCATGTAATAGGTGGCACTTTAAATCCAAATGCTGCATACTTACTTATACGAGGCATGAAGACATTGCATCTTCGTGTTCAGCAGCAGAATTCCTCAGCTTTGAGGATGGCAAAGCTTTTAGAGGCTCATCCTAAGATCAAATGTGTTTACTACCCTGGGTTACCAAGTCATCCTGAGCATAATCTTGCCAAAAGACAGATGAGTGGTTTCGGGGGCGTAGTTAGTTTCGAGGTTGATGGGGATTTAACGACCACCATAAAATTCATTGATTCATTAAAAATTCCATATATTGCTCCATCATTTGGTGGGTGCGAGAGTATCATAGACCAGCCAGCAATAATGTCATATTGGGATCTTAACCAAACAGAGAGGCTCAAGTATGGGATAAAGGACAATTTGGTGCGGTTCAGCATTGGAATTGAAGAGTTTGAGGATTTGAAGGCCGATATTCTGCAGGGTCTTGAGGCCATTTAA

Protein sequence

MAVSSHAALIGSASFEGLSDPNSGVAACLRTHSYARFAQPKSVKGTLASHALSSSRIFKFPPNFLRQLSNKARRNCSNIGVAQVVAASWSNNSPPSSSAAAAAATSVDATAAASVPAASDAVALEGCVGNESLKVEEDLGDSRTSVFHSDGSVAIHAGERFGRGRVDDAITTPVVNTSAYFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMFLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLELALKENKVSIFFTESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATKYLGGHNDVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAKLLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI
BLAST of Carg16984 vs. NCBI nr
Match: XP_022931717.1 (cystathionine gamma-synthase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 990.3 bits (2559), Expect = 2.5e-285
Identity = 527/538 (97.96%), Postives = 529/538 (98.33%), Query Frame = 0

Query: 1   MAVSSHAALIGSASFEGLSDPNSGVAACLRTHSYARFAQPKSVKGTLASHALSSSRIFKF 60
           MAVSSHAALIGSASFEGLSDPNSGVAACLRTHSYARFAQPKSVKGTLASHALSSSRIFKF
Sbjct: 1   MAVSSHAALIGSASFEGLSDPNSGVAACLRTHSYARFAQPKSVKGTLASHALSSSRIFKF 60

Query: 61  PPNFLRQLSNKARRNCSNIGVAQVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPAASD 120
           PPNFLRQLSNKARRNCSNIGVAQVV        XXXXXXXXXXXXXXXXXXXXXX AASD
Sbjct: 61  PPNFLRQLSNKARRNCSNIGVAQVVAASWSNNSXXXXXXXXXXXXXXXXXXXXXXXAASD 120

Query: 121 AVALEGCVGNESLKVEEDLGDSRTSVFHSDGSVAIHAGERFGRGRVDDAITTPVVNTSAY 180
           AVALEGCVGNESLKVEEDLGDSRTSVFHSDGSVAIHAGERFGRGRVDDAITTPVVNTSAY
Sbjct: 121 AVALEGCVGNESLKVEEDLGDSRTSVFHSDGSVAIHAGERFGRGRVDDAITTPVVNTSAY 180

Query: 181 FFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMFL 240
           FFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMFL
Sbjct: 181 FFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMFL 240

Query: 241 ALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLELALKENKVSIFFTE 300
           ALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATT+DPADMNSLE+ALKENKVSIFFTE
Sbjct: 241 ALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTVDPADMNSLEVALKENKVSIFFTE 300

Query: 301 SPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATKYLGGHN 360
           SPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATKYLGGHN
Sbjct: 301 SPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATKYLGGHN 360

Query: 361 DVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAKL 420
           DVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAKL
Sbjct: 361 DVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAKL 420

Query: 421 LEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIAP 480
           LEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIAP
Sbjct: 421 LEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIAP 480

Query: 481 SFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI 539
           SFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI
Sbjct: 481 SFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI 538

BLAST of Carg16984 vs. NCBI nr
Match: XP_023531479.1 (cystathionine gamma-synthase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 969.9 bits (2506), Expect = 3.5e-279
Identity = 529/539 (98.14%), Postives = 531/539 (98.52%), Query Frame = 0

Query: 1   MAVSSHAALIGSASFEGLSDPNSGVAACLRTHSYARFAQPKSVKGTLASHALSSSRIFKF 60
           MAVSS AALIGSASFEG  DPNSGVAACLRTHSYARFAQPKSVKGTLASHALSSSRIFKF
Sbjct: 1   MAVSSRAALIGSASFEGRCDPNSGVAACLRTHSYARFAQPKSVKGTLASHALSSSRIFKF 60

Query: 61  PPNFLRQLSNKARRNCSNIGVAQVV-XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPAAS 120
           PPNFLRQLSNKARRNCSNIGVAQVV XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   S
Sbjct: 61  PPNFLRQLSNKARRNCSNIGVAQVVAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXS 120

Query: 121 DAVALEGCVGNESLKVEEDLGDSRTSVFHSDGSVAIHAGERFGRGRVDDAITTPVVNTSA 180
           DAVALEGCVGNESLKVEEDLGDSRTSVF+SDGSVAIHAGERFGRGRVDDAITTPVVNTSA
Sbjct: 121 DAVALEGCVGNESLKVEEDLGDSRTSVFNSDGSVAIHAGERFGRGRVDDAITTPVVNTSA 180

Query: 181 YFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMF 240
           YFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMF
Sbjct: 181 YFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMF 240

Query: 241 LALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLELALKENKVSIFFT 300
           LALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLE+ALKENKVSIFFT
Sbjct: 241 LALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLEVALKENKVSIFFT 300

Query: 301 ESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATKYLGGH 360
           ESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATKYL GH
Sbjct: 301 ESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATKYLAGH 360

Query: 361 NDVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAK 420
           NDVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAK
Sbjct: 361 NDVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAK 420

Query: 421 LLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIA 480
           LLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIA
Sbjct: 421 LLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIA 480

Query: 481 PSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI 539
           PSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI
Sbjct: 481 PSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI 539

BLAST of Carg16984 vs. NCBI nr
Match: XP_023007592.1 (cystathionine gamma-synthase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 962.6 bits (2487), Expect = 5.6e-277
Identity = 516/538 (95.91%), Postives = 519/538 (96.47%), Query Frame = 0

Query: 1   MAVSSHAALIGSASFEGLSDPNSGVAACLRTHSYARFAQPKSVKGTLASHALSSSRIFKF 60
           MAVSSHA LIGSASF+G  DPNSGVA CLRTHSYARFAQPKSVKGTLASHALSSSRIFKF
Sbjct: 1   MAVSSHATLIGSASFDGRYDPNSGVAPCLRTHSYARFAQPKSVKGTLASHALSSSRIFKF 60

Query: 61  PPNFLRQLSNKARRNCSNIGVAQVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPAASD 120
           PPNFLRQLSNKARRNCSNIGVAQVV        XXXXXXXXXXXXXXXXXXXXXX     
Sbjct: 61  PPNFLRQLSNKARRNCSNIGVAQVV--AASWSNXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 121 AVALEGCVGNESLKVEEDLGDSRTSVFHSDGSVAIHAGERFGRGRVDDAITTPVVNTSAY 180
           AVALEGCVGNESLKVEEDLGDSRTSVF SDGSVAIHAGERFGRGRVDDAITTPVVNTSAY
Sbjct: 121 AVALEGCVGNESLKVEEDLGDSRTSVFDSDGSVAIHAGERFGRGRVDDAITTPVVNTSAY 180

Query: 181 FFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMFL 240
           FFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMFL
Sbjct: 181 FFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMFL 240

Query: 241 ALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLELALKENKVSIFFTE 300
           ALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLE+ALKENKVSIFFTE
Sbjct: 241 ALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLEVALKENKVSIFFTE 300

Query: 301 SPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATKYLGGHN 360
           SPTNPFLRCVDIKLVSELCHQHGALVCIDGTFAT LNQKALSLGADLVIHSATKYLGGHN
Sbjct: 301 SPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATPLNQKALSLGADLVIHSATKYLGGHN 360

Query: 361 DVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAKL 420
           DV+AGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAKL
Sbjct: 361 DVLAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAKL 420

Query: 421 LEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIAP 480
           LEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIAP
Sbjct: 421 LEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIAP 480

Query: 481 SFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI 539
           SFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI
Sbjct: 481 SFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI 536

BLAST of Carg16984 vs. NCBI nr
Match: XP_022983752.1 (cystathionine gamma-synthase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 879.0 bits (2270), Expect = 8.1e-252
Identity = 474/539 (87.94%), Postives = 494/539 (91.65%), Query Frame = 0

Query: 1   MAVSSHAALIGSASFEGLSDPNSGVAACLRTHSYARFA-QPKSVKGTLASHALSSSRIFK 60
           MA SSHA L+G AS E  S P+  VA   R HSY RF  + KSVK T+AS+ALSSSRIFK
Sbjct: 1   MAGSSHARLLGPAS-ECRSCPDLAVAGRFRGHSYGRFTHEAKSVKATVASYALSSSRIFK 60

Query: 61  FPPNFLRQLSNKARRNCSNIGVAQVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPAAS 120
           FPPNF+RQLS KARRNCSNIGVAQVV        XXXXXXXXXXXXXXXXXXXXXX    
Sbjct: 61  FPPNFVRQLSTKARRNCSNIGVAQVVAASWSNNSXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 121 DAVALEGCVGNESLKVEEDLGDSRTSVFHSDGSVAIHAGERFGRGRVDDAITTPVVNTSA 180
               LEG VG ESLKVEE LGDSRTSVF  DGSVAIHAGERFGRGRVDDAITTPVVNTSA
Sbjct: 121 XXXXLEGSVGTESLKVEEGLGDSRTSVFDCDGSVAIHAGERFGRGRVDDAITTPVVNTSA 180

Query: 181 YFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMF 240
           YFFKKTADL+DFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMM 
Sbjct: 181 YFFKKTADLLDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMML 240

Query: 241 LALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLELALKENKVSIFFT 300
           LALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMN+LE+ALKEN VS+FFT
Sbjct: 241 LALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNALEVALKENNVSLFFT 300

Query: 301 ESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATKYLGGH 360
           ESPTNPFLRCVDIKLVSE+CHQHGALVCIDGTFAT LNQKALSLGADLV+HSATKY+GGH
Sbjct: 301 ESPTNPFLRCVDIKLVSEMCHQHGALVCIDGTFATPLNQKALSLGADLVLHSATKYIGGH 360

Query: 361 NDVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAK 420
           NDV+AGCVSGSLKLVSEIRNLHHVIGG LNPNAAYL+IRGMKTLHLRVQQQNSSALRMAK
Sbjct: 361 NDVLAGCVSGSLKLVSEIRNLHHVIGGALNPNAAYLIIRGMKTLHLRVQQQNSSALRMAK 420

Query: 421 LLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIA 480
           LLEAHPKIKCVYYPGLPSHPEHNLAKRQM+GFGGVVSFEVDGD+TTTIKFIDSLKIPYIA
Sbjct: 421 LLEAHPKIKCVYYPGLPSHPEHNLAKRQMTGFGGVVSFEVDGDITTTIKFIDSLKIPYIA 480

Query: 481 PSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI 539
           PSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIE+FEDLKAD+LQ L+AI
Sbjct: 481 PSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEDFEDLKADVLQALDAI 538

BLAST of Carg16984 vs. NCBI nr
Match: XP_023528162.1 (cystathionine gamma-synthase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 877.1 bits (2265), Expect = 3.1e-251
Identity = 467/539 (86.64%), Postives = 489/539 (90.72%), Query Frame = 0

Query: 1   MAVSSHAALIGSASFEGLSDPNSGVAACLRTHSYARFA-QPKSVKGTLASHALSSSRIFK 60
           MA SSHA L+G AS +  S P+  VA   R HSY+RF  + KSVK T+AS+ALSSSRIFK
Sbjct: 1   MAGSSHARLLGPAS-DCRSCPDLAVAGRFRGHSYSRFTHEAKSVKATVASYALSSSRIFK 60

Query: 61  FPPNFLRQLSNKARRNCSNIGVAQVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPAAS 120
           FPPNF+RQLS KARRNCSNIGVAQVV              XXXXXXXXXXXXXXXX    
Sbjct: 61  FPPNFVRQLSTKARRNCSNIGVAQVVAASWSNNSSPFQSAXXXXXXXXXXXXXXXXXXXX 120

Query: 121 DAVALEGCVGNESLKVEEDLGDSRTSVFHSDGSVAIHAGERFGRGRVDDAITTPVVNTSA 180
               LEG VG ESLKVEE LGDSRTSVF  DGSVAIHAGERFGRGRVDDAITTPVVNTSA
Sbjct: 121 XXXXLEGSVGTESLKVEEGLGDSRTSVFDCDGSVAIHAGERFGRGRVDDAITTPVVNTSA 180

Query: 181 YFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMF 240
           YFFKKTADL+DFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMM 
Sbjct: 181 YFFKKTADLLDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMML 240

Query: 241 LALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLELALKENKVSIFFT 300
           LALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMN+LE+ALKEN VS+FFT
Sbjct: 241 LALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNALEVALKENNVSLFFT 300

Query: 301 ESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATKYLGGH 360
           ESPTNPFLRCVDIKLVSE+CH+HGALVCIDGTFAT LNQKALSLGADLV+HSATKY+GGH
Sbjct: 301 ESPTNPFLRCVDIKLVSEMCHRHGALVCIDGTFATPLNQKALSLGADLVLHSATKYIGGH 360

Query: 361 NDVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAK 420
           NDV+AGCVSGSLKLVSEIRNLHHVIGG LNPNAAYL+IRGMKTLHLRVQQQNSSALRMAK
Sbjct: 361 NDVLAGCVSGSLKLVSEIRNLHHVIGGALNPNAAYLIIRGMKTLHLRVQQQNSSALRMAK 420

Query: 421 LLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIA 480
           LLEAHPKIKCVYYPGLPSHPEHNLAKRQM+GFGGVVSFEVDGD+TTTIKFIDSLKIPYIA
Sbjct: 421 LLEAHPKIKCVYYPGLPSHPEHNLAKRQMTGFGGVVSFEVDGDITTTIKFIDSLKIPYIA 480

Query: 481 PSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI 539
           PSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIE+FEDLKADILQ L+AI
Sbjct: 481 PSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEDFEDLKADILQALDAI 538

BLAST of Carg16984 vs. TAIR10
Match: AT3G01120.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein)

HSP 1 Score: 714.1 bits (1842), Expect = 6.3e-206
Identity = 397/564 (70.39%), Postives = 447/564 (79.26%), Query Frame = 0

Query: 1   MAVSSH--AALIGSASFEGL---SDPNSGVAACLRTHSYARFAQPKSVKGTLASHALSSS 60
           MAVSS     +  S+S  G    SDP+  V + +   S  R      +  +    A  SS
Sbjct: 1   MAVSSFQCPTIFSSSSISGFQCRSDPDL-VGSPVGGSSRRRVHASAGISSSFTGDAGLSS 60

Query: 61  RIFKFPPNFLRQLSNKARRNCSNIGVAQVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120
           RI +FPPNF+RQLS KARRNCSNIGVAQ+V     XXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 61  RILRFPPNFVRQLSIKARRNCSNIGVAQIVAAKWSXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 121 PAASDAVALEGCVGNESLK----------------VEEDLG-----DSRTSVFHSDGSVA 180
                                              + E +G     DS+ S   SDGS+ 
Sbjct: 121 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGIREKIGSVQLTDSKHSFLSSDGSLT 180

Query: 181 IHAGERFGRGRVDDAITTPVVNTSAYFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEE 240
           +HAGER GRG V DAITTPVVNTSAYFFKKTA+LIDFKEKR+VSFEYGRYGNPTT+V E+
Sbjct: 181 VHAGERLGRGIVTDAITTPVVNTSAYFFKKTAELIDFKEKRSVSFEYGRYGNPTTVVLED 240

Query: 241 KISALEGAESTLIVASGMCASTMMFLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITA 300
           KISALEGAESTL++ASGMCAST+M LALVPAGGHIVTTTDCYRKTRIF+E  LPK+GIT 
Sbjct: 241 KISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRKTRIFMENFLPKLGITV 300

Query: 301 TTIDPADMNSLELALKENKVSIFFTESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFAT 360
           T IDPAD+  LE A+ E KVS+FFTESPTNPFLRCVDI+LVS++CH+ G LVCIDGTFAT
Sbjct: 301 TVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSKICHKRGTLVCIDGTFAT 360

Query: 361 ALNQKALSLGADLVIHSATKYLGGHNDVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAY 420
            LNQKAL+LGADLV+HSATKY+GGHNDV+AGC+ GSLKLVSEIRNLHHV+GGTLNPNAAY
Sbjct: 361 PLNQKALALGADLVVHSATKYIGGHNDVLAGCICGSLKLVSEIRNLHHVLGGTLNPNAAY 420

Query: 421 LLIRGMKTLHLRVQQQNSSALRMAKLLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGV 480
           L+IRGMKTLHLRVQQQNS+A RMA++LEAHPK+  VYYPGLPSHPEH LAKRQM+GFGGV
Sbjct: 421 LIIRGMKTLHLRVQQQNSTAFRMAEILEAHPKVSHVYYPGLPSHPEHELAKRQMTGFGGV 480

Query: 481 VSFEVDGDLTTTIKFIDSLKIPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNL 539
           VSFE+DGD+ TTIKF+DSLKIPYIAPSFGGCESI+DQPAIMSYWDL Q ERLKYGIKDNL
Sbjct: 481 VSFEIDGDIETTIKFVDSLKIPYIAPSFGGCESIVDQPAIMSYWDLPQEERLKYGIKDNL 540

BLAST of Carg16984 vs. TAIR10
Match: AT1G33320.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein)

HSP 1 Score: 538.9 bits (1387), Expect = 3.6e-153
Identity = 259/381 (67.98%), Postives = 313/381 (82.15%), Query Frame = 0

Query: 158 GERFGRGRVDDAITTPVVNTSAYFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKIS 217
           GER  R     +ITT VVNT+ YFFK T +LIDFKEKR   +EY RYGNPTT+  EEKIS
Sbjct: 33  GERLRRDMKTSSITTLVVNTTTYFFKDTTELIDFKEKRIDLYEYARYGNPTTMALEEKIS 92

Query: 218 ALEGAESTLIVASGMCASTMMFLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTI 277
            LEGAESTL++ASGM AS +M LALVP  GHIV T DCY++TRIF+E  L K+GIT T I
Sbjct: 93  VLEGAESTLVMASGMYASNVMLLALVPTNGHIVATKDCYKETRIFMENFLTKLGITVTFI 152

Query: 278 DPADMNSLELALKENKVSIFFTESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALN 337
           D  D+  L+  +  ++VS+FFTESPTNPFLRCVDIKLVS++CH+ G LVCID T AT +N
Sbjct: 153 DSDDIAGLQTLVNNHEVSLFFTESPTNPFLRCVDIKLVSKICHRRGTLVCIDATIATPIN 212

Query: 338 QKALSLGADLVIHSATKYLGGHNDVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLI 397
           QK L+LGADLV HSATKY+GGHND +AG +SGS++LVS+IRNLH ++GGTLNPNAAYLLI
Sbjct: 213 QKTLALGADLVHHSATKYIGGHNDFLAGSISGSMELVSKIRNLHKLLGGTLNPNAAYLLI 272

Query: 398 RGMKTLHLRVQQQNSSALRMAKLLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSF 457
           RGMKT+HLRV+QQNS+ ++MA++LEAHPK+  VYY GLPSHPEH +AKRQM+G GG++SF
Sbjct: 273 RGMKTMHLRVRQQNSTGMKMAQVLEAHPKVSRVYYLGLPSHPEHLIAKRQMTGIGGLISF 332

Query: 458 EVDGDLTTTIKFIDSLKIPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRF 517
           E+DGDL TTIKFID+LKIPY+A SFGGCES++DQ A    WD+ + ERLK G +DNLVRF
Sbjct: 333 EIDGDLKTTIKFIDALKIPYLAASFGGCESLVDQLA-TGIWDIPREERLKDGFQDNLVRF 392

Query: 518 SIGIEEFEDLKADILQGLEAI 539
           S GIE+FED+KAD+LQ LE I
Sbjct: 393 SFGIEDFEDIKADVLQALETI 412

BLAST of Carg16984 vs. TAIR10
Match: AT3G57050.1 (cystathionine beta-lyase)

HSP 1 Score: 217.6 bits (553), Expect = 1.8e-56
Identity = 126/369 (34.15%), Postives = 204/369 (55.28%), Query Frame = 0

Query: 168 DAITTPVVNTSAYFFKKTADLIDFKEKRAVS---FEYGRYGNPTTIVAEEKISALEGAES 227
           DA++TP+  T+            FK+  A+    ++Y R GNPT    E  ++ L+ A+ 
Sbjct: 102 DAMSTPLYQTAT-----------FKQPSAIENGPYDYTRSGNPTRDALESLLAKLDKADR 161

Query: 228 TLIVASGMCASTMMFLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNS 287
                SGM A + +   L+  G  IV   D Y  +   +  ++P+ G+    ++   ++ 
Sbjct: 162 AFCFTSGMAALSAV-THLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDE 221

Query: 288 LELALKENKVSIFFTESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALNQKALSLG 347
           +  A+   +  + + ESPTNP  +  DI+ +SE+ H  GALV +D +  + +  + L LG
Sbjct: 222 VAAAIGP-QTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELG 281

Query: 348 ADLVIHSATKYLGGHNDVIAG--CVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKT 407
           AD+V+HSATK++ GH+DV+AG   V G  KL  E+  L +  G  L P   +L +RG+KT
Sbjct: 282 ADIVMHSATKFIAGHSDVMAGVLAVKGE-KLAKEVYFLQNSEGSGLAPFDCWLCLRGIKT 341

Query: 408 LHLRVQQQNSSALRMAKLLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGD 467
           + LR+++Q  +A ++A  L +HP++K VYY GLP HP H+L   Q  G G V SF + G 
Sbjct: 342 MALRIEKQQENARKIAMYLSSHPRVKKVYYAGLPDHPGHHLHFSQAKGAGSVFSF-ITGS 401

Query: 468 LTTTIKFIDSLKIPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIE 527
           +  +   +++ K   IA SFG  +S+I  P  MS+  +    R   G+ ++LVR S GIE
Sbjct: 402 VALSKHLVETTKYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDLVRISAGIE 455

Query: 528 EFEDLKADI 532
           + +DL +D+
Sbjct: 462 DVDDLISDL 455

BLAST of Carg16984 vs. TAIR10
Match: AT1G64660.1 (methionine gamma-lyase)

HSP 1 Score: 164.1 bits (414), Expect = 2.4e-40
Identity = 102/334 (30.54%), Postives = 180/334 (53.89%), Query Frame = 0

Query: 199 FEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMFLALVPAGGHIVTTTDCYRK 258
           + Y R+ NPT +    +++ALEG ++    +SGM A + + L L  +GGH+V  +  Y  
Sbjct: 93  YVYSRHFNPTVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYGG 152

Query: 259 TRIFIETILPK-MGITATTIDPADMNSLELALKENKVSIFFTESPTNPFLRCVDIKLVSE 318
           T   +   LP+   IT + +D  D  ++  A+ E +  + + ES  NP L   DI  +S 
Sbjct: 153 THALLSHFLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELSR 212

Query: 319 LCHQHGALVCIDGTFATALNQKALSLGADLVIHSATKYLGGHNDVIAGCVSGSLKLVSEI 378
           + H+ G  V +D TFA  +   A  LGAD+V+HS +K++ G  D+IAG V GS  LV E+
Sbjct: 213 MAHEKGVTVVVDNTFAPMVLSPA-KLGADVVVHSISKFISGGADIIAGAVCGSENLVKEM 272

Query: 379 RNLH----HVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMA-KLLEAHPKIKCVYY 438
            +L      ++G T+N   A+ L   +  L LR+++ +  A   A ++ +   K   V Y
Sbjct: 273 MDLRGGSLMLLGPTMNAKVAFELSERIPHLGLRMREHSHRAQVYAERMRDLGMK---VIY 332

Query: 439 PGLPSHPEHNLAKRQMS---GFGGVVSFEVDGD--LTTTIKFI-DSLKIPYIAPSFGGCE 498
           PGL +HP+H L K  ++   G+GG++S +++ +      + ++ ++ +  ++A S G  E
Sbjct: 333 PGLETHPQHKLFKGMVNRDYGYGGLLSIDMETEEKANKLMAYLQNATQFGFMAVSLGYYE 392

Query: 499 SIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIG 521
           +++      +  +L+ +++   GI   LVR S+G
Sbjct: 393 TLMSCSGSSTSSELDPSQKEAAGISPGLVRMSVG 422

BLAST of Carg16984 vs. Swiss-Prot
Match: sp|P55217|CGS1_ARATH (Cystathionine gamma-synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CGS1 PE=1 SV=3)

HSP 1 Score: 714.1 bits (1842), Expect = 1.1e-204
Identity = 397/564 (70.39%), Postives = 447/564 (79.26%), Query Frame = 0

Query: 1   MAVSSH--AALIGSASFEGL---SDPNSGVAACLRTHSYARFAQPKSVKGTLASHALSSS 60
           MAVSS     +  S+S  G    SDP+  V + +   S  R      +  +    A  SS
Sbjct: 1   MAVSSFQCPTIFSSSSISGFQCRSDPDL-VGSPVGGSSRRRVHASAGISSSFTGDAGLSS 60

Query: 61  RIFKFPPNFLRQLSNKARRNCSNIGVAQVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120
           RI +FPPNF+RQLS KARRNCSNIGVAQ+V     XXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 61  RILRFPPNFVRQLSIKARRNCSNIGVAQIVAAKWSXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 121 PAASDAVALEGCVGNESLK----------------VEEDLG-----DSRTSVFHSDGSVA 180
                                              + E +G     DS+ S   SDGS+ 
Sbjct: 121 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGIREKIGSVQLTDSKHSFLSSDGSLT 180

Query: 181 IHAGERFGRGRVDDAITTPVVNTSAYFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEE 240
           +HAGER GRG V DAITTPVVNTSAYFFKKTA+LIDFKEKR+VSFEYGRYGNPTT+V E+
Sbjct: 181 VHAGERLGRGIVTDAITTPVVNTSAYFFKKTAELIDFKEKRSVSFEYGRYGNPTTVVLED 240

Query: 241 KISALEGAESTLIVASGMCASTMMFLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITA 300
           KISALEGAESTL++ASGMCAST+M LALVPAGGHIVTTTDCYRKTRIF+E  LPK+GIT 
Sbjct: 241 KISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRKTRIFMENFLPKLGITV 300

Query: 301 TTIDPADMNSLELALKENKVSIFFTESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFAT 360
           T IDPAD+  LE A+ E KVS+FFTESPTNPFLRCVDI+LVS++CH+ G LVCIDGTFAT
Sbjct: 301 TVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSKICHKRGTLVCIDGTFAT 360

Query: 361 ALNQKALSLGADLVIHSATKYLGGHNDVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAY 420
            LNQKAL+LGADLV+HSATKY+GGHNDV+AGC+ GSLKLVSEIRNLHHV+GGTLNPNAAY
Sbjct: 361 PLNQKALALGADLVVHSATKYIGGHNDVLAGCICGSLKLVSEIRNLHHVLGGTLNPNAAY 420

Query: 421 LLIRGMKTLHLRVQQQNSSALRMAKLLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGV 480
           L+IRGMKTLHLRVQQQNS+A RMA++LEAHPK+  VYYPGLPSHPEH LAKRQM+GFGGV
Sbjct: 421 LIIRGMKTLHLRVQQQNSTAFRMAEILEAHPKVSHVYYPGLPSHPEHELAKRQMTGFGGV 480

Query: 481 VSFEVDGDLTTTIKFIDSLKIPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNL 539
           VSFE+DGD+ TTIKF+DSLKIPYIAPSFGGCESI+DQPAIMSYWDL Q ERLKYGIKDNL
Sbjct: 481 VSFEIDGDIETTIKFVDSLKIPYIAPSFGGCESIVDQPAIMSYWDLPQEERLKYGIKDNL 540

BLAST of Carg16984 vs. Swiss-Prot
Match: sp|Q9C876|CGS2_ARATH (Probable cystathionine gamma-synthase 2 OS=Arabidopsis thaliana OX=3702 GN=CGS2 PE=3 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 6.5e-152
Identity = 259/381 (67.98%), Postives = 313/381 (82.15%), Query Frame = 0

Query: 158 GERFGRGRVDDAITTPVVNTSAYFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKIS 217
           GER  R     +ITT VVNT+ YFFK T +LIDFKEKR   +EY RYGNPTT+  EEKIS
Sbjct: 33  GERLRRDMKTSSITTLVVNTTTYFFKDTTELIDFKEKRIDLYEYARYGNPTTMALEEKIS 92

Query: 218 ALEGAESTLIVASGMCASTMMFLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTI 277
            LEGAESTL++ASGM AS +M LALVP  GHIV T DCY++TRIF+E  L K+GIT T I
Sbjct: 93  VLEGAESTLVMASGMYASNVMLLALVPTNGHIVATKDCYKETRIFMENFLTKLGITVTFI 152

Query: 278 DPADMNSLELALKENKVSIFFTESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALN 337
           D  D+  L+  +  ++VS+FFTESPTNPFLRCVDIKLVS++CH+ G LVCID T AT +N
Sbjct: 153 DSDDIAGLQTLVNNHEVSLFFTESPTNPFLRCVDIKLVSKICHRRGTLVCIDATIATPIN 212

Query: 338 QKALSLGADLVIHSATKYLGGHNDVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLI 397
           QK L+LGADLV HSATKY+GGHND +AG +SGS++LVS+IRNLH ++GGTLNPNAAYLLI
Sbjct: 213 QKTLALGADLVHHSATKYIGGHNDFLAGSISGSMELVSKIRNLHKLLGGTLNPNAAYLLI 272

Query: 398 RGMKTLHLRVQQQNSSALRMAKLLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSF 457
           RGMKT+HLRV+QQNS+ ++MA++LEAHPK+  VYY GLPSHPEH +AKRQM+G GG++SF
Sbjct: 273 RGMKTMHLRVRQQNSTGMKMAQVLEAHPKVSRVYYLGLPSHPEHLIAKRQMTGIGGLISF 332

Query: 458 EVDGDLTTTIKFIDSLKIPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRF 517
           E+DGDL TTIKFID+LKIPY+A SFGGCES++DQ A    WD+ + ERLK G +DNLVRF
Sbjct: 333 EIDGDLKTTIKFIDALKIPYLAASFGGCESLVDQLA-TGIWDIPREERLKDGFQDNLVRF 392

Query: 518 SIGIEEFEDLKADILQGLEAI 539
           S GIE+FED+KAD+LQ LE I
Sbjct: 393 SFGIEDFEDIKADVLQALETI 412

BLAST of Carg16984 vs. Swiss-Prot
Match: sp|P0A4K2|METC_LACLA (Cystathionine beta-lyase OS=Lactococcus lactis subsp. lactis (strain IL1403) OX=272623 GN=metC PE=3 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 9.3e-74
Identity = 159/383 (41.51%), Postives = 231/383 (60.31%), Query Frame = 0

Query: 155 IHAGERFGRGRVDDAITTPVVNTSAYFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEE 214
           IH G      +   A++ P+  TS Y   K   L   KE     +EY R GNPT    EE
Sbjct: 9   IHGG--ISTDKTTGAVSVPIYQTSTY---KQNGLGQPKE-----YEYSRSGNPTRHALEE 68

Query: 215 KISALEGAESTLIVASGMCASTMMFLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITA 274
            I+ LEG       +SG+ A     L+L  AG HI+   D Y  T   ++ +L K GI  
Sbjct: 69  LIADLEGGVQGFAFSSGL-AGIHAVLSLFSAGDHIILADDVYGGTFRLMDKVLTKTGIIY 128

Query: 275 TTIDPADMNSLELALKENKVSIFFTESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFAT 334
             +D ++++ L+ A KE   +I+F E+P+NP L+ +DIK +S +   H AL  +D TFAT
Sbjct: 129 DLVDLSNLDDLKAAFKEETKAIYF-ETPSNPLLKVLDIKEISAIAKAHDALTLVDNTFAT 188

Query: 335 ALNQKALSLGADLVIHSATKYLGGHNDVIAGCV-SGSLKLVSEIRNLHHVIGGTLNPNAA 394
              Q+ ++LGAD+V+HSATKYLGGH+DV+AG V + S +L SEI  L + IG  L P  +
Sbjct: 189 PYLQQPIALGADIVLHSATKYLGGHSDVVAGLVTTNSKELASEIGFLQNSIGAVLGPQDS 248

Query: 395 YLLIRGMKTLHLRVQQQNSSALRMAKLLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGG 454
           +L+ RG+KTL LR++  +++A ++A+ LE    +  VYYPGL SHP H +AK+QMS FGG
Sbjct: 249 WLVQRGIKTLALRMEAHSANAQKIAEFLETSKAVSKVYYPGLNSHPGHEIAKKQMSAFGG 308

Query: 455 VVSFEVDGDLTTTIKFIDSLKIPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDN 514
           ++SFE+  D      F+++L    +A S GG ES+I+ PA+M++  + +  R + GIKD 
Sbjct: 309 MISFELT-DENAVKDFVENLSYFTLAESLGGVESLIEVPAVMTHASIPKELREEIGIKDG 368

Query: 515 LVRFSIGIEEFEDLKADILQGLE 537
           L+R S+G+E  EDL  DI + LE
Sbjct: 369 LIRLSVGVEAIEDLLTDIKEALE 378

BLAST of Carg16984 vs. Swiss-Prot
Match: sp|A2RM21|METC_LACLM (Cystathionine beta-lyase OS=Lactococcus lactis subsp. cremoris (strain MG1363) OX=416870 GN=metC PE=1 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 9.3e-74
Identity = 159/383 (41.51%), Postives = 231/383 (60.31%), Query Frame = 0

Query: 155 IHAGERFGRGRVDDAITTPVVNTSAYFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEE 214
           IH G      +   A++ P+  TS Y   K   L   KE     +EY R GNPT    EE
Sbjct: 9   IHGG--ISTDKTTGAVSVPIYQTSTY---KQNGLGQPKE-----YEYSRSGNPTRHALEE 68

Query: 215 KISALEGAESTLIVASGMCASTMMFLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITA 274
            I+ LEG       +SG+ A     L+L  AG HI+   D Y  T   ++ +L K GI  
Sbjct: 69  LIADLEGGVQGFAFSSGL-AGIHAVLSLFSAGDHIILADDVYGGTFRLMDKVLTKTGIIY 128

Query: 275 TTIDPADMNSLELALKENKVSIFFTESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFAT 334
             +D ++++ L+ A KE   +I+F E+P+NP L+ +DIK +S +   H AL  +D TFAT
Sbjct: 129 DLVDLSNLDDLKAAFKEETKAIYF-ETPSNPLLKVLDIKEISAIAKAHDALTLVDNTFAT 188

Query: 335 ALNQKALSLGADLVIHSATKYLGGHNDVIAGCV-SGSLKLVSEIRNLHHVIGGTLNPNAA 394
              Q+ ++LGAD+V+HSATKYLGGH+DV+AG V + S +L SEI  L + IG  L P  +
Sbjct: 189 PYLQQPIALGADIVLHSATKYLGGHSDVVAGLVTTNSKELASEIGFLQNSIGAVLGPQDS 248

Query: 395 YLLIRGMKTLHLRVQQQNSSALRMAKLLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGG 454
           +L+ RG+KTL LR++  +++A ++A+ LE    +  VYYPGL SHP H +AK+QMS FGG
Sbjct: 249 WLVQRGIKTLALRMEAHSANAQKIAEFLETSKAVSKVYYPGLNSHPGHEIAKKQMSAFGG 308

Query: 455 VVSFEVDGDLTTTIKFIDSLKIPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDN 514
           ++SFE+  D      F+++L    +A S GG ES+I+ PA+M++  + +  R + GIKD 
Sbjct: 309 MISFELT-DENAVKDFVENLSYFTLAESLGGVESLIEVPAVMTHASIPKELREEIGIKDG 368

Query: 515 LVRFSIGIEEFEDLKADILQGLE 537
           L+R S+G+E  EDL  DI + LE
Sbjct: 369 LIRLSVGVEAIEDLLTDIKEALE 378

BLAST of Carg16984 vs. Swiss-Prot
Match: sp|Q1M0P5|METB_HELPX (Cystathionine gamma-synthase OS=Helicobacter pylori OX=210 GN=metB PE=1 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 3.5e-73
Identity = 154/385 (40.00%), Postives = 223/385 (57.92%), Query Frame = 0

Query: 155 IHAGERFGRGRVDDAITTPVVNTSAYFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEE 214
           IH G          A++ P+  TS Y      D I   +     +EY R GNPT    EE
Sbjct: 8   IHGG--ISEDATTGAVSVPIYQTSTY----RQDAIGHHK----GYEYSRSGNPTRFALEE 67

Query: 215 KISALEGAESTLIVASGMCASTMMFLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITA 274
            I+ LEG       ASG+     +F +L+ +G H++   D Y  T      +L K G++ 
Sbjct: 68  LIADLEGGVKGFAFASGLAGIHAVF-SLLQSGDHVLLGDDVYGGTFRLFNKVLVKNGLSC 127

Query: 275 TTIDPADMNSLELALKENKVSIFFTESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFAT 334
           T ID +D++ ++ A+K N  +++  E+P+NP L+  D+   + +   HG L  +D TFAT
Sbjct: 128 TIIDTSDLSQIKKAIKPNTKALYL-ETPSNPLLKITDLAQCASVAKDHGLLTIVDNTFAT 187

Query: 335 ALNQKALSLGADLVIHSATKYLGGHNDVIAGCV-SGSLKLVSEIRNLHHVIGGTLNPNAA 394
              Q  L LGAD+V+HS TKYLGGH+DV+AG V + +  L  EI    + IGG L P  +
Sbjct: 188 PYYQNPLLLGADIVVHSGTKYLGGHSDVVAGLVTTNNEALAQEIAFFQNAIGGVLGPQDS 247

Query: 395 YLLIRGMKTLHLRVQQQNSSALRMAKLLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGG 454
           +LL RG+KTL LR+Q    +AL +A+ LE HPK++ VYYPGLP+HP + LAK+QM GF G
Sbjct: 248 WLLQRGIKTLGLRMQAHQKNALCVAEFLEKHPKVERVYYPGLPTHPNYELAKKQMRGFSG 307

Query: 455 VVSFEVDGDLTTTIKFIDSLKIPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDN 514
           ++SF +  D   T  F++SLK+  +  S GG ES++  PA M++  + +T+R   GI+D 
Sbjct: 308 MLSFTLKNDSEAT-PFVESLKLFILGESLGGVESLVGVPAFMTHACIPKTQREAAGIRDG 367

Query: 515 LVRFSIGIEEFEDLKADILQGLEAI 539
           LVR S+GIE  +DL  D+ Q    I
Sbjct: 368 LVRLSVGIEHEQDLLEDLEQAFAKI 379

BLAST of Carg16984 vs. TrEMBL
Match: tr|A0A0A0LG06|A0A0A0LG06_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G824870 PE=3 SV=1)

HSP 1 Score: 869.8 bits (2246), Expect = 3.3e-249
Identity = 479/544 (88.05%), Postives = 506/544 (93.01%), Query Frame = 0

Query: 1   MAVSSHAALIGSASFEGLSDPNSGVAACLRTHSYARFA-QPKSVKGTLASHALSSSRIFK 60
           MAVSSHA L+ S +F+  SDP   +A+  R ++Y RFA +PKSVKGT+ASH LSS  IFK
Sbjct: 1   MAVSSHARLL-SPTFDCRSDPVLALASAHRRNTYGRFAHEPKSVKGTVASHGLSSFPIFK 60

Query: 61  FPPNFLRQLSNKARRNCSNIGVAQVV----XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120
           FPPNF+RQLSNKARRNCSNIGVAQVV    XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 61  FPPNFVRQLSNKARRNCSNIGVAQVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 121 PAASDAVALEGCVGNESLKVEE-DLGDSRTSVFHSDGSVAIHAGERFGRGRVDDAITTPV 180
              SDAVALEG VGNESLKVEE  L DSRTSVF SDGSVAIHAGERFGRGR DDAITTPV
Sbjct: 121 XXXSDAVALEGSVGNESLKVEEKGLVDSRTSVFDSDGSVAIHAGERFGRGRADDAITTPV 180

Query: 181 VNTSAYFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCA 240
           VNTSAYFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCA
Sbjct: 181 VNTSAYFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCA 240

Query: 241 STMMFLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLELALKENKV 300
           STMM LALVPAGGHIVTTTDCYRKTRIFIET+LPKMGITATTIDPAD+N+LE+ALKEN V
Sbjct: 241 STMMLLALVPAGGHIVTTTDCYRKTRIFIETMLPKMGITATTIDPADINALEVALKENNV 300

Query: 301 SIFFTESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATK 360
           S+FFTESPTNPFLRCVDIKLVSE+CHQHGALVCIDGTFAT LNQKALSLGADL++HSATK
Sbjct: 301 SLFFTESPTNPFLRCVDIKLVSEMCHQHGALVCIDGTFATPLNQKALSLGADLILHSATK 360

Query: 361 YLGGHNDVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSA 420
           Y+GGHNDV+AGC+SGSLKLVSEIRNLHHVIGG LNPNAAYL+IRGMKT+HLRVQQQNSS 
Sbjct: 361 YIGGHNDVLAGCISGSLKLVSEIRNLHHVIGGALNPNAAYLIIRGMKTMHLRVQQQNSSG 420

Query: 421 LRMAKLLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLK 480
           L+MA LLEAHPKIKCVYYPGLPSHPEHNLAKRQM+GFGGVVSFEVDGDL TT KFIDSLK
Sbjct: 421 LKMAMLLEAHPKIKCVYYPGLPSHPEHNLAKRQMTGFGGVVSFEVDGDLMTTAKFIDSLK 480

Query: 481 IPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQG 539
           IPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIE+FEDLKADILQ 
Sbjct: 481 IPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEDFEDLKADILQA 540

BLAST of Carg16984 vs. TrEMBL
Match: tr|A0A1S3B7V3|A0A1S3B7V3_CUCME (cystathionine gamma-synthase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486979 PE=3 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 5.6e-249
Identity = 478/544 (87.87%), Postives = 505/544 (92.83%), Query Frame = 0

Query: 1   MAVSSHAALIGSASFEGLSDPNSGVAACLRTHSYARFA-QPKSVKGTLASHALSSSRIFK 60
           MAVSSHA L+        SDP   VA+  R ++Y RFA +PKSVKGT+ASH LSS  IFK
Sbjct: 1   MAVSSHARLLSPTFDSRSSDPLLAVASAHRRNTYGRFAHEPKSVKGTVASHGLSSFPIFK 60

Query: 61  FPPNFLRQLSNKARRNCSNIGVAQVV---XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXP 120
           FPPNF+RQLSNKARRNCSNIGVAQVV   XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 
Sbjct: 61  FPPNFVRQLSNKARRNCSNIGVAQVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120

Query: 121 AASDAVALEGCVG-NESLKVEE-DLGDSRTSVFHSDGSVAIHAGERFGRGRVDDAITTPV 180
            ASDAVALEGCVG NESLKVEE  L DSRTSVF SDGSVAIHAGERFGRGR DDAITTPV
Sbjct: 121 XASDAVALEGCVGNNESLKVEEKGLVDSRTSVFDSDGSVAIHAGERFGRGRADDAITTPV 180

Query: 181 VNTSAYFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCA 240
           VNTSAYFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCA
Sbjct: 181 VNTSAYFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCA 240

Query: 241 STMMFLALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLELALKENKV 300
           STMM LALVPAGGHIVTTTDCYRKTRIFIET+LPKMGITATTIDPADMN+LE+ALKEN V
Sbjct: 241 STMMLLALVPAGGHIVTTTDCYRKTRIFIETMLPKMGITATTIDPADMNALEVALKENNV 300

Query: 301 SIFFTESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATK 360
           S+FFTESPTNPFLRCVDIKLVSE+CH+HGALVCIDGTFAT LNQKAL+LGADL++HSATK
Sbjct: 301 SLFFTESPTNPFLRCVDIKLVSEMCHRHGALVCIDGTFATPLNQKALALGADLILHSATK 360

Query: 361 YLGGHNDVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSA 420
           Y+GGHNDV+AGC+SG LKLVSEIRNLHHVIGG LNPNAAYL+IRGMKT+HLRVQQQNSS 
Sbjct: 361 YIGGHNDVLAGCISGPLKLVSEIRNLHHVIGGALNPNAAYLIIRGMKTMHLRVQQQNSSG 420

Query: 421 LRMAKLLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLK 480
           L+MA+LLEAHPKIKCVYYPGLPSHPEH+LAKRQM+GFGGVVSFEVDGDLTTT KFIDSLK
Sbjct: 421 LKMARLLEAHPKIKCVYYPGLPSHPEHSLAKRQMTGFGGVVSFEVDGDLTTTAKFIDSLK 480

Query: 481 IPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQG 539
           IPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIE+FEDLKADILQ 
Sbjct: 481 IPYIAPSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEDFEDLKADILQA 540

BLAST of Carg16984 vs. TrEMBL
Match: tr|A0A2I4GY06|A0A2I4GY06_9ROSI (cystathionine gamma-synthase 1, chloroplastic-like isoform X1 OS=Juglans regia OX=51240 GN=LOC109011873 PE=3 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 3.2e-212
Identity = 405/539 (75.14%), Postives = 454/539 (84.23%), Query Frame = 0

Query: 14  SFEGLSDPNSGVAACL---RTHSYARFAQP-KSVKGTLASHALSSSRIFKFPPNFLRQLS 73
           SFE  S+P+   A  +   R  S+ RFA   K  +GT + H   SS IF+FPPNF+RQLS
Sbjct: 11  SFESCSEPDFSGAPNIGGDRKRSHGRFAGGLKPARGTASFHGSLSSLIFRFPPNFVRQLS 70

Query: 74  NKARRNCSNIGVAQVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXP----AASDAVAL- 133
            KARRNCSNIGVA +V     XXXXXXXXXXXXXXXXXXXXXX   P    AA D VAL 
Sbjct: 71  TKARRNCSNIGVAHIVAASWSXXXXXXXXXXXXXXXXXXXXXXPIPPSVDAAAVDGVALI 130

Query: 134 -----EGCVGNESLKVEEDLGDSRTSVFHSDGSVAIHAGERFGRGRVDDAITTPVVNTSA 193
                   +G  +L++ E    S+ SVF  DGS+ IHAGER GRG V D ITTPVVNTSA
Sbjct: 131 EKEVDRSVIGGLNLEL-EGFPKSKPSVFSFDGSLTIHAGERSGRGIVTDGITTPVVNTSA 190

Query: 194 YFFKKTADLIDFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMF 253
           YFF  TA LIDFKEKR  SFEYGRYGNPTT+V+EEKISALEGAESTLI+ASGMCAST++ 
Sbjct: 191 YFFDNTAQLIDFKEKRRASFEYGRYGNPTTVVSEEKISALEGAESTLIMASGMCASTVLL 250

Query: 254 LALVPAGGHIVTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLELALKENKVSIFFT 313
           +ALVPAGGH+VTTTDCYRKTRIFIET+LP+MGITAT IDPAD+ +LE AL +NKVS+FFT
Sbjct: 251 MALVPAGGHLVTTTDCYRKTRIFIETVLPRMGITATVIDPADVGALESALNKNKVSLFFT 310

Query: 314 ESPTNPFLRCVDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATKYLGGH 373
           ESPTNPFLRCVDIKLVSELCH  GALVCIDGTFAT LNQKAL+LGADLV+HSATKYLGGH
Sbjct: 311 ESPTNPFLRCVDIKLVSELCHGRGALVCIDGTFATPLNQKALALGADLVLHSATKYLGGH 370

Query: 374 NDVIAGCVSGSLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAK 433
           NDV+AGC+SGS+KLVSE+RNLHH++GG LNPNAAYL+IRGMKTLHLRVQQQNS+AL MAK
Sbjct: 371 NDVLAGCISGSMKLVSEVRNLHHILGGALNPNAAYLIIRGMKTLHLRVQQQNSTALGMAK 430

Query: 434 LLEAHPKIKCVYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIA 493
           +LEAHPK+K VYYPGLPSHPEH+LAK+QM+GFGGVVSFEVDGDL TTIKF+DSLKIPYIA
Sbjct: 431 ILEAHPKVKRVYYPGLPSHPEHDLAKQQMTGFGGVVSFEVDGDLQTTIKFVDSLKIPYIA 490

Query: 494 PSFGGCESIIDQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI 539
           PSFGGCESI+DQPAIMSYWDL Q+ERLKYGIKDNLVR+S G+E+FEDLKADILQ LE I
Sbjct: 491 PSFGGCESIVDQPAIMSYWDLPQSERLKYGIKDNLVRYSFGVEDFEDLKADILQALENI 548

BLAST of Carg16984 vs. TrEMBL
Match: tr|A0A2P5XQ51|A0A2P5XQ51_GOSBA (Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=GOBAR_AA15211 PE=3 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 2.3e-210
Identity = 389/529 (73.53%), Postives = 439/529 (82.99%), Query Frame = 0

Query: 13  ASFEGLSDPN-SGVAACLRTHSYARFAQPKSVKGTL--ASHALSSSRIFKFPPNFLRQLS 72
           +SFE  SDP+ SG      T    R    + V  +L        SS IF+FPPNF+RQLS
Sbjct: 17  SSFECRSDPDFSGTP----TGEKPRIGSNRRVTASLFCTGGGGLSSLIFRFPPNFVRQLS 76

Query: 73  NKARRNCSNIGVAQVVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPAASDAVALEGCVG 132
            KARRNCSNIGVAQVV                 XXXXXXXXXXXXXP +     +EGC+ 
Sbjct: 77  VKARRNCSNIGVAQVV--AASWSNGPTSGYPSSXXXXXXXXXXXXXPVSDGVAVVEGCID 136

Query: 133 NESLKVEEDLGDSRTSVFHSDGSVAIHAGERFGRGRVDDAITTPVVNTSAYFFKKTADLI 192
           N+S+++  D  DS+TS   SDGS+ +HAGER GRG V DAITTPVVNTSAYFFKKT +LI
Sbjct: 137 NDSVQI-GDSYDSKTSFLSSDGSITVHAGERLGRGIVTDAITTPVVNTSAYFFKKTQELI 196

Query: 193 DFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMFLALVPAGGHI 252
           DFKEKR  SFEYGRYGNPTT+VAEEKISALEGAESTLI+ASGMCAST+M +ALVPAGGHI
Sbjct: 197 DFKEKRHTSFEYGRYGNPTTVVAEEKISALEGAESTLIMASGMCASTVMLMALVPAGGHI 256

Query: 253 VTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLELALKENKVSIFFTESPTNPFLRC 312
           VTTTDCYRKTRIFIETILPKMGI AT IDPAD++ LE AL +NKVS+FFTESPTNPFLRC
Sbjct: 257 VTTTDCYRKTRIFIETILPKMGILATVIDPADVDGLESALNKNKVSLFFTESPTNPFLRC 316

Query: 313 VDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATKYLGGHNDVIAGCVSG 372
           VDI+ VS+LCH  G LVCIDGTFAT LNQKAL LGADLV+HSATK++GGHNDV+AGC+SG
Sbjct: 317 VDIEKVSKLCHSKGTLVCIDGTFATPLNQKALVLGADLVLHSATKFIGGHNDVLAGCISG 376

Query: 373 SLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAKLLEAHPKIKC 432
           S KLV+EIR LHH++GGTLNPNAAYL+IRGMKTLHLRVQQQNS+ALRMA++LEAHP++KC
Sbjct: 377 SEKLVTEIRTLHHILGGTLNPNAAYLIIRGMKTLHLRVQQQNSTALRMAEVLEAHPRVKC 436

Query: 433 VYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIAPSFGGCESII 492
           VYYPGLPSHPEH +AKRQM+GFGGVVSFEVDGDL TTIKF+D+LKIPYIAPSFGGCESI+
Sbjct: 437 VYYPGLPSHPEHEIAKRQMTGFGGVVSFEVDGDLMTTIKFVDALKIPYIAPSFGGCESIV 496

Query: 493 DQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI 539
           DQPAIMSYWDL+Q ER KYGI DNL+RFS G+E+FEDLKADILQ LE I
Sbjct: 497 DQPAIMSYWDLSQAERSKYGILDNLIRFSFGVEDFEDLKADILQALETI 538

BLAST of Carg16984 vs. TrEMBL
Match: tr|A0A2I4E3L0|A0A2I4E3L0_9ROSI (cystathionine gamma-synthase 1, chloroplastic-like OS=Juglans regia OX=51240 GN=LOC108985953 PE=3 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 8.7e-210
Identity = 401/529 (75.80%), Postives = 445/529 (84.12%), Query Frame = 0

Query: 14  SFEGLSDPN-SGVAACLRTHSYARFAQPKSVKGTLASHALSSSRIFKFPPNFLRQLSNKA 73
           SFE  S+P+ SG     R        +  S +GT++ H   SS IF+FPPNF+RQLS KA
Sbjct: 12  SFECRSEPDFSGAPNIGRGDKKPSHGRLYSARGTVSFHWGLSSLIFRFPPNFVRQLSTKA 71

Query: 74  RRNCSNIGVAQVV---XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPAASDAVALEGCVG 133
           RRNCSNIGVAQ+V           XXXXXXXXXXXXXXXXXXX   PAA D V L     
Sbjct: 72  RRNCSNIGVAQIVAASWSNNPASPXXXXXXXXXXXXXXXXXXXLVDPAAEDDVTLVEKEV 131

Query: 134 NESLKVEEDLGDSRTSVFHSDGSVAIHAGERFGRGRVDDAITTPVVNTSAYFFKKTADLI 193
           + S+ V E L  S+ SVF  DGS+AIHAGER GRG V DAITTPVVNTSAYFF  TA LI
Sbjct: 132 DRSVGV-EGLPKSKPSVFSYDGSLAIHAGERSGRGIVTDAITTPVVNTSAYFFDNTAQLI 191

Query: 194 DFKEKRAVSFEYGRYGNPTTIVAEEKISALEGAESTLIVASGMCASTMMFLALVPAGGHI 253
           DFKEKR  SFEYGRYGNPTT+VAEEKISALEGAESTLI+ASGMCAST++ LALVPAGGH+
Sbjct: 192 DFKEKRRASFEYGRYGNPTTVVAEEKISALEGAESTLIMASGMCASTVLLLALVPAGGHL 251

Query: 254 VTTTDCYRKTRIFIETILPKMGITATTIDPADMNSLELALKENKVSIFFTESPTNPFLRC 313
           VTTTDCYRKTRIFIETILP+MGI AT IDPAD+ +LE AL EN VS+FFTESPTNPFLRC
Sbjct: 252 VTTTDCYRKTRIFIETILPRMGIKATVIDPADVGALESALNENNVSLFFTESPTNPFLRC 311

Query: 314 VDIKLVSELCHQHGALVCIDGTFATALNQKALSLGADLVIHSATKYLGGHNDVIAGCVSG 373
           VDIK VSELCHQ GALVCIDGTFAT LNQKAL+LGADLV+HSATKYLGGHNDV+AGC+SG
Sbjct: 312 VDIKHVSELCHQKGALVCIDGTFATPLNQKALALGADLVLHSATKYLGGHNDVLAGCISG 371

Query: 374 SLKLVSEIRNLHHVIGGTLNPNAAYLLIRGMKTLHLRVQQQNSSALRMAKLLEAHPKIKC 433
           S+KLVSE+RNLHH++GG LNPNAAYL+IRGMKTLHLRVQQQNS+AL MA++LEAHPK+K 
Sbjct: 372 SMKLVSEVRNLHHILGGALNPNAAYLIIRGMKTLHLRVQQQNSTALGMAEILEAHPKVKR 431

Query: 434 VYYPGLPSHPEHNLAKRQMSGFGGVVSFEVDGDLTTTIKFIDSLKIPYIAPSFGGCESII 493
           VYYPGL SHPE++LAKRQM+GFGGVVSFEVDGDL TTIKF+DSL+IPYIAPSFGGCESI+
Sbjct: 432 VYYPGLQSHPEYDLAKRQMTGFGGVVSFEVDGDLQTTIKFVDSLRIPYIAPSFGGCESIV 491

Query: 494 DQPAIMSYWDLNQTERLKYGIKDNLVRFSIGIEEFEDLKADILQGLEAI 539
           DQPAIMSYWDL Q+ERL+YGIKDNLVRFS G+E FEDLKADILQ LE I
Sbjct: 492 DQPAIMSYWDLPQSERLEYGIKDNLVRFSFGVEHFEDLKADILQALETI 539

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022931717.12.5e-28597.96cystathionine gamma-synthase 1, chloroplastic-like [Cucurbita moschata][more]
XP_023531479.13.5e-27998.14cystathionine gamma-synthase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_023007592.15.6e-27795.91cystathionine gamma-synthase 1, chloroplastic-like [Cucurbita maxima][more]
XP_022983752.18.1e-25287.94cystathionine gamma-synthase 1, chloroplastic-like [Cucurbita maxima][more]
XP_023528162.13.1e-25186.64cystathionine gamma-synthase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G01120.16.3e-20670.39Pyridoxal phosphate (PLP)-dependent transferases superfamily protein[more]
AT1G33320.13.6e-15367.98Pyridoxal phosphate (PLP)-dependent transferases superfamily protein[more]
AT3G57050.11.8e-5634.15cystathionine beta-lyase[more]
AT1G64660.12.4e-4030.54methionine gamma-lyase[more]
Match NameE-valueIdentityDescription
sp|P55217|CGS1_ARATH1.1e-20470.39Cystathionine gamma-synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
sp|Q9C876|CGS2_ARATH6.5e-15267.98Probable cystathionine gamma-synthase 2 OS=Arabidopsis thaliana OX=3702 GN=CGS2 ... [more]
sp|P0A4K2|METC_LACLA9.3e-7441.51Cystathionine beta-lyase OS=Lactococcus lactis subsp. lactis (strain IL1403) OX=... [more]
sp|A2RM21|METC_LACLM9.3e-7441.51Cystathionine beta-lyase OS=Lactococcus lactis subsp. cremoris (strain MG1363) O... [more]
sp|Q1M0P5|METB_HELPX3.5e-7340.00Cystathionine gamma-synthase OS=Helicobacter pylori OX=210 GN=metB PE=1 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A0A0LG06|A0A0A0LG06_CUCSA3.3e-24988.05Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G824870 PE=3 SV=1[more]
tr|A0A1S3B7V3|A0A1S3B7V3_CUCME5.6e-24987.87cystathionine gamma-synthase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034... [more]
tr|A0A2I4GY06|A0A2I4GY06_9ROSI3.2e-21275.14cystathionine gamma-synthase 1, chloroplastic-like isoform X1 OS=Juglans regia O... [more]
tr|A0A2P5XQ51|A0A2P5XQ51_GOSBA2.3e-21073.53Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=GOBAR_AA15211 PE=3 SV... [more]
tr|A0A2I4E3L0|A0A2I4E3L0_9ROSI8.7e-21075.80cystathionine gamma-synthase 1, chloroplastic-like OS=Juglans regia OX=51240 GN=... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0030170pyridoxal phosphate binding
Vocabulary: INTERPRO
TermDefinition
IPR015424PyrdxlP-dep_Trfase
IPR015422PyrdxlP-dep_Trfase_dom1
IPR015421PyrdxlP-dep_Trfase_major
IPR000277Cys/Met-Metab_PyrdxlP-dep_enz
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003824 catalytic activity
molecular_function GO:0030170 pyridoxal phosphate binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16984-RACarg16984-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePFAMPF01053Cys_Met_Meta_PPcoord: 153..536
e-value: 8.3E-140
score: 465.5
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePROSITEPS00868CYS_MET_METAB_PPcoord: 346..360
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymeCDDcd00614CGS_likecoord: 168..536
e-value: 2.29352E-176
score: 504.429
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3DG3DSA:3.40.640.10coord: 143..400
e-value: 8.4E-84
score: 282.6
IPR015422Pyridoxal phosphate-dependent transferase domain 1GENE3DG3DSA:3.90.1150.10coord: 401..538
e-value: 1.1E-53
score: 183.1
NoneNo IPR availablePANTHERPTHR43379:SF1CYSTATHIONINE GAMMA-SYNTHASE 1, CHLOROPLASTIC-RELATEDcoord: 43..538
NoneNo IPR availablePANTHERPTHR43379FAMILY NOT NAMEDcoord: 43..538
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILYSSF53383PLP-dependent transferasescoord: 175..537

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg16984CmaCh11G012030Cucurbita maxima (Rimu)carcmaB0651
Carg16984CmoCh11G012350Cucurbita moschata (Rifu)carcmoB0632
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None