Carg16904 (gene) Silver-seed gourd

NameCarg16904
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionsubtilisin-like protease SBT1.2
LocationCucurbita_argyrosperma_scaffold_105 : 776790 .. 778973 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGATTCATGGATTTCAACTCCCAAATGGTTTTTTCCACCATTTTCCTCTGTTTTCTTTTGCTTCAAGTTCAAGCAAACGCTTCCAATCTCCAAACTTACATGATTCAACTCCATCCACAAGGATTAACCAGCTCTGTTTTCGAATCTAAGCTTCAATGGCATCTTTCTTTTCTTGAACAAACGCTATCTGTTGAAGAAGACTGTTCTTCTCGCTTGCTTTATTCATATTCGAACGCCATGGAAGGATTTGCAGCTCAGTTATCTGAAACTGAGCTTGAGTATTTGAAGAAGTTGGCTGATGTTGTGGCTGTAAGAGCTGATAGAAAGTATGAAATTCAGACAACTTATTCACATAAGTTCTTGGGGCTTAGTGTTTGCACACAAGGTGTTTGTTATAAGTCCTCAATGGGCCAAGGGGCAATCGTTGGGGTTCTTGACACGGGAGTTTGGCCTGAGAGTCCAAGCTTTAGTGATTCCAAAATGCCCCCAATTCCACAAAAATGGCGAGGGGGTTGCCAAGAAGGGCAAGATTTTAATTCCTCAAGTTGTAATAGGAAACTCATTGGTGCTAAGTTCTTCATCAAAGGACATCATGTGGCTTCGTCGCCGCCTTCCGATATCGTCCAGGAATATGTCTCCCCAAGAGACTCCCATGGCCATGGGACTCACACTTCTTCTACAGCTGCTGGAGCTTCGGTTGCCGGAGCGAGCGTATTTGGTAATGGAGCAGGTGTGGCGCAGGGGATGGCCCCGGGCGCCCACATTGCTGTGTACAAGGTTTGCTGGTTTAGTGGCTGCTATAGCTCTGATATTGTAGCGGCCATGGATTCCGCAATAAGAGATGGTGTTGACATTCTCTCCCTTTCGCTTGGTGGCTTCCCACTTCCGTTTTTCGACGACAGTATCGCCATTGGCAGTTTTCGAGCAATGCAGCATGGCATCTCAGTCATTTGTGCAGCAGGAAACAATGGCCCAACTCAAAGCTCTGTCGCCAATGTAGCTCCTTGGATCACCACCATTGGTGCAGGCACGCTCGATCGAACATTCCCAGCTATAGTTCGACTAAGCAATGGCGAAGCCATTCCCGGCGAATCAATGTACCCTGGAAACAAGTTCAATCAAGCTACCAAGGAGCTTGAGTTGGTTTACTTGACTGGAGGGAAAATGGGAAGTGAACTTTGCTTAAAAGGGTCTCTTCCACGAGAACAAGTAGAAGGAAAAATGGTGGTTTGCGACCGTGGCGTCAACGGCCGATCCGAAAAGGGGCAGATTGTGAAGGAATCTGGAGGAGCTGCTATGATCCTTGCAAATACAGAGATAAATCTAGAGGAAGACTTGGTCGATGTTCATGTTTTGCCGGCCACGTTGATCGGATTTGCGGAGGCGAATCGGTTAAAAGCTTACATTAACACAACAACCGATCCAAAAGCCCGAATCCAATTTGGAGGAACTGTGATAGGAAGAACAAGAGCTCCATCTGTAGCTCAGTTTTCATCAAGAGGACCAAGCCCCACAAATCCTTCAACGCTCAAACCTGATGTAATTGCTCCTGGGGTCAACATTATAGCAGCTTGGCCTCAAAACCTTGCTCCAACTGGCCTTCTAGAAGATTCTAGAAGGTCAAACTTCACTGTCATGTCAGGAACATCCATGTCTTGTCCCCATGTCAGTGGAATTGCAGCTCTAATCCATTCAGCTCATCCAAAATGGACACCGGCAGCTATCAAATCAGCCATCATGACAACCGCCGATGTTACCGATCGTTTCGGGAAACCGATTCTCGATGGCAACAAACCAGCTAGTGTTTTTGCAATGGGAGCTGGTCATGTAAACCCAACAAGAGCCATTGATCCTGGCTTGGTCTATGATATCAAGCCATATGATTATGTCATTCATCTTTGTGTTCTTGGCTACACTCATTCAGAAATCTTCATCATTACCCACATGAATGTGAGCTGCCACAAAGTTCTTCAGATGAACAAAGGCTTCACATTGAACTATCCCTCCATGTCTGTCGTTTTTAAGCACGGGACAACGAGTAAGATGGTTTCGAGATGGTTGACGAATGTCGGGAGCCCGAATTCCGTCTATGAAGTGGAAGTAACCGCGCCTGAAGGAGTGAGCGTTCAAGTTAAGCCTCGGCGCTTGGTGTTCAAACATGTGAACGAAAGTTTGAGTTATAAG

mRNA sequence

AGATTCATGGATTTCAACTCCCAAATGGTTTTTTCCACCATTTTCCTCTGTTTTCTTTTGCTTCAAGTTCAAGCAAACGCTTCCAATCTCCAAACTTACATGATTCAACTCCATCCACAAGGATTAACCAGCTCTGTTTTCGAATCTAAGCTTCAATGGCATCTTTCTTTTCTTGAACAAACGCTATCTGTTGAAGAAGACTGTTCTTCTCGCTTGCTTTATTCATATTCGAACGCCATGGAAGGATTTGCAGCTCAGTTATCTGAAACTGAGCTTGAGTATTTGAAGAAGTTGGCTGATGTTGTGGCTGTAAGAGCTGATAGAAAGTATGAAATTCAGACAACTTATTCACATAAGTTCTTGGGGCTTAGTGTTTGCACACAAGGTGTTTGTTATAAGTCCTCAATGGGCCAAGGGGCAATCGTTGGGGTTCTTGACACGGGAGTTTGGCCTGAGAGTCCAAGCTTTAGTGATTCCAAAATGCCCCCAATTCCACAAAAATGGCGAGGGGGTTGCCAAGAAGGGCAAGATTTTAATTCCTCAAGTTGTAATAGGAAACTCATTGGTGCTAAGTTCTTCATCAAAGGACATCATGTGGCTTCGTCGCCGCCTTCCGATATCGTCCAGGAATATGTCTCCCCAAGAGACTCCCATGGCCATGGGACTCACACTTCTTCTACAGCTGCTGGAGCTTCGGTTGCCGGAGCGAGCGTATTTGGTAATGGAGCAGGTGTGGCGCAGGGGATGGCCCCGGGCGCCCACATTGCTGTGTACAAGGTTTGCTGGTTTAGTGGCTGCTATAGCTCTGATATTGTAGCGGCCATGGATTCCGCAATAAGAGATGGTGTTGACATTCTCTCCCTTTCGCTTGGTGGCTTCCCACTTCCGTTTTTCGACGACAGTATCGCCATTGGCAGTTTTCGAGCAATGCAGCATGGCATCTCAGTCATTTGTGCAGCAGGAAACAATGGCCCAACTCAAAGCTCTGTCGCCAATGTAGCTCCTTGGATCACCACCATTGGTGCAGGCACGCTCGATCGAACATTCCCAGCTATAGTTCGACTAAGCAATGGCGAAGCCATTCCCGGCGAATCAATGTACCCTGGAAACAAGTTCAATCAAGCTACCAAGGAGCTTGAGTTGGTTTACTTGACTGGAGGGAAAATGGGAAGTGAACTTTGCTTAAAAGGGTCTCTTCCACGAGAACAAGTAGAAGGAAAAATGGTGGTTTGCGACCGTGGCGTCAACGGCCGATCCGAAAAGGGGCAGATTGTGAAGGAATCTGGAGGAGCTGCTATGATCCTTGCAAATACAGAGATAAATCTAGAGGAAGACTTGGTCGATGTTCATGTTTTGCCGGCCACGTTGATCGGATTTGCGGAGGCGAATCGGTTAAAAGCTTACATTAACACAACAACCGATCCAAAAGCCCGAATCCAATTTGGAGGAACTGTGATAGGAAGAACAAGAGCTCCATCTGTAGCTCAGTTTTCATCAAGAGGACCAAGCCCCACAAATCCTTCAACGCTCAAACCTGATGTAATTGCTCCTGGGGTCAACATTATAGCAGCTTGGCCTCAAAACCTTGCTCCAACTGGCCTTCTAGAAGATTCTAGAAGGTCAAACTTCACTGTCATGTCAGGAACATCCATGTCTTGTCCCCATGTCAGTGGAATTGCAGCTCTAATCCATTCAGCTCATCCAAAATGGACACCGGCAGCTATCAAATCAGCCATCATGACAACCGCCGATGTTACCGATCGTTTCGGGAAACCGATTCTCGATGGCAACAAACCAGCTAGTGTTTTTGCAATGGGAGCTGGTCATGTAAACCCAACAAGAGCCATTGATCCTGGCTTGGTCTATGATATCAAGCCATATGATTATGTCATTCATCTTTGTGTTCTTGGCTACACTCATTCAGAAATCTTCATCATTACCCACATGAATGTGAGCTGCCACAAAGTTCTTCAGATGAACAAAGGCTTCACATTGAACTATCCCTCCATGTCTGTCGTTTTTAAGCACGGGACAACGAGTAAGATGGTTTCGAGATGGTTGACGAATGTCGGGAGCCCGAATTCCGTCTATGAAGTGGAAGTAACCGCGCCTGAAGGAGTGAGCGTTCAAGTTAAGCCTCGGCGCTTGGTGTTCAAACATGTGAACGAAAGTTTGAGTTATAAG

Coding sequence (CDS)

ATGGATTTCAACTCCCAAATGGTTTTTTCCACCATTTTCCTCTGTTTTCTTTTGCTTCAAGTTCAAGCAAACGCTTCCAATCTCCAAACTTACATGATTCAACTCCATCCACAAGGATTAACCAGCTCTGTTTTCGAATCTAAGCTTCAATGGCATCTTTCTTTTCTTGAACAAACGCTATCTGTTGAAGAAGACTGTTCTTCTCGCTTGCTTTATTCATATTCGAACGCCATGGAAGGATTTGCAGCTCAGTTATCTGAAACTGAGCTTGAGTATTTGAAGAAGTTGGCTGATGTTGTGGCTGTAAGAGCTGATAGAAAGTATGAAATTCAGACAACTTATTCACATAAGTTCTTGGGGCTTAGTGTTTGCACACAAGGTGTTTGTTATAAGTCCTCAATGGGCCAAGGGGCAATCGTTGGGGTTCTTGACACGGGAGTTTGGCCTGAGAGTCCAAGCTTTAGTGATTCCAAAATGCCCCCAATTCCACAAAAATGGCGAGGGGGTTGCCAAGAAGGGCAAGATTTTAATTCCTCAAGTTGTAATAGGAAACTCATTGGTGCTAAGTTCTTCATCAAAGGACATCATGTGGCTTCGTCGCCGCCTTCCGATATCGTCCAGGAATATGTCTCCCCAAGAGACTCCCATGGCCATGGGACTCACACTTCTTCTACAGCTGCTGGAGCTTCGGTTGCCGGAGCGAGCGTATTTGGTAATGGAGCAGGTGTGGCGCAGGGGATGGCCCCGGGCGCCCACATTGCTGTGTACAAGGTTTGCTGGTTTAGTGGCTGCTATAGCTCTGATATTGTAGCGGCCATGGATTCCGCAATAAGAGATGGTGTTGACATTCTCTCCCTTTCGCTTGGTGGCTTCCCACTTCCGTTTTTCGACGACAGTATCGCCATTGGCAGTTTTCGAGCAATGCAGCATGGCATCTCAGTCATTTGTGCAGCAGGAAACAATGGCCCAACTCAAAGCTCTGTCGCCAATGTAGCTCCTTGGATCACCACCATTGGTGCAGGCACGCTCGATCGAACATTCCCAGCTATAGTTCGACTAAGCAATGGCGAAGCCATTCCCGGCGAATCAATGTACCCTGGAAACAAGTTCAATCAAGCTACCAAGGAGCTTGAGTTGGTTTACTTGACTGGAGGGAAAATGGGAAGTGAACTTTGCTTAAAAGGGTCTCTTCCACGAGAACAAGTAGAAGGAAAAATGGTGGTTTGCGACCGTGGCGTCAACGGCCGATCCGAAAAGGGGCAGATTGTGAAGGAATCTGGAGGAGCTGCTATGATCCTTGCAAATACAGAGATAAATCTAGAGGAAGACTTGGTCGATGTTCATGTTTTGCCGGCCACGTTGATCGGATTTGCGGAGGCGAATCGGTTAAAAGCTTACATTAACACAACAACCGATCCAAAAGCCCGAATCCAATTTGGAGGAACTGTGATAGGAAGAACAAGAGCTCCATCTGTAGCTCAGTTTTCATCAAGAGGACCAAGCCCCACAAATCCTTCAACGCTCAAACCTGATGTAATTGCTCCTGGGGTCAACATTATAGCAGCTTGGCCTCAAAACCTTGCTCCAACTGGCCTTCTAGAAGATTCTAGAAGGTCAAACTTCACTGTCATGTCAGGAACATCCATGTCTTGTCCCCATGTCAGTGGAATTGCAGCTCTAATCCATTCAGCTCATCCAAAATGGACACCGGCAGCTATCAAATCAGCCATCATGACAACCGCCGATGTTACCGATCGTTTCGGGAAACCGATTCTCGATGGCAACAAACCAGCTAGTGTTTTTGCAATGGGAGCTGGTCATGTAAACCCAACAAGAGCCATTGATCCTGGCTTGGTCTATGATATCAAGCCATATGATTATGTCATTCATCTTTGTGTTCTTGGCTACACTCATTCAGAAATCTTCATCATTACCCACATGAATGTGAGCTGCCACAAAGTTCTTCAGATGAACAAAGGCTTCACATTGAACTATCCCTCCATGTCTGTCGTTTTTAAGCACGGGACAACGAGTAAGATGGTTTCGAGATGGTTGACGAATGTCGGGAGCCCGAATTCCGTCTATGAAGTGGAAGTAACCGCGCCTGAAGGAGTGAGCGTTCAAGTTAAGCCTCGGCGCTTGGTGTTCAAACATGTGAACGAAAGTTTGAGTTATAAG

Protein sequence

MDFNSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTLSVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLGLSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIPGESMYPGNKFNQATKELELVYLTGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFGGTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSNFTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASVFAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFTLNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVNESLSYK
BLAST of Carg16904 vs. NCBI nr
Match: XP_022963543.1 (subtilisin-like protease SBT1.2 [Cucurbita moschata])

HSP 1 Score: 1395.9 bits (3612), Expect = 0.0e+00
Identity = 719/726 (99.04%), Postives = 721/726 (99.31%), Query Frame = 0

Query: 1   MDFNSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDF SQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL
Sbjct: 1   MDFYSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLG 120
           SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKL DVVAVRADRKYEIQTTYSHKFLG
Sbjct: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120

Query: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
           LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSF DSKMPPIPQKWRGGCQEGQDFNSSS
Sbjct: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFGDSKMPPIPQKWRGGCQEGQDFNSSS 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNG 240
           CNRKLIGAKFFIKGHHVASSPPSD VQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 360
           AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP
Sbjct: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKFNQATKELELVYLTGG+MGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFG 480
           QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFG
Sbjct: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540
           GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN
Sbjct: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540

Query: 541 FTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           FTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV
Sbjct: 541 FTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVN 720
           FTLNYPSMSVVFKHGTTSKMVSR LTNVGSPNSVYEVEVTAPEGVSV+VKPRRLVFKHVN
Sbjct: 661 FTLNYPSMSVVFKHGTTSKMVSRRLTNVGSPNSVYEVEVTAPEGVSVRVKPRRLVFKHVN 720

Query: 721 ESLSYK 727
           ESLSYK
Sbjct: 721 ESLSYK 726

BLAST of Carg16904 vs. NCBI nr
Match: XP_022967188.1 (subtilisin-like protease SBT1.2 [Cucurbita maxima])

HSP 1 Score: 1385.2 bits (3584), Expect = 0.0e+00
Identity = 694/726 (95.59%), Postives = 701/726 (96.56%), Query Frame = 0

Query: 1   MDFNSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDFNSQMVFSTIFLCFL LQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL
Sbjct: 1   MDFNSQMVFSTIFLCFLWLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLG 120
           SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKL DVVAVRADRKYEIQTTYSHKFLG
Sbjct: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120

Query: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
           LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS
Sbjct: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNG 240
           CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGT                  NG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGALVAGASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300
            GVAQGMAPG HIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI
Sbjct: 241 VGVAQGMAPGTHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 360
           AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGE IP
Sbjct: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEPIP 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKFNQATKELELVYLTGG+MGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFG 480
           QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTT+PKARIQFG
Sbjct: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540
           GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN
Sbjct: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540

Query: 541 FTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           FTVMSGTSM+CPHVSGIAALI SAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV
Sbjct: 541 FTVMSGTSMACPHVSGIAALIQSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPTRAIDPGLVYDIKP+DYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTRAIDPGLVYDIKPFDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVN 720
           FTLNYPSM+VVFKHGTTSKMVSR LTNVGSPNSVYEVEVTAPEGVSV+VKPRRLVFKHVN
Sbjct: 661 FTLNYPSMAVVFKHGTTSKMVSRRLTNVGSPNSVYEVEVTAPEGVSVRVKPRRLVFKHVN 720

Query: 721 ESLSYK 727
           +SLSYK
Sbjct: 721 QSLSYK 726

BLAST of Carg16904 vs. NCBI nr
Match: XP_023553603.1 (subtilisin-like protease SBT1.2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1374.8 bits (3557), Expect = 0.0e+00
Identity = 709/726 (97.66%), Postives = 718/726 (98.90%), Query Frame = 0

Query: 1   MDFNSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDFNSQM F TIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL
Sbjct: 1   MDFNSQMGFPTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLG 120
           SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKL DVVAVRADRKYEIQTTYSHKFLG
Sbjct: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120

Query: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
             VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS
Sbjct: 121 --VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNG 240
           CNRKLIGAKFFIKGHHVASSPPSD VQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDD+I
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNI 300

Query: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 360
           AIGSFRA+QHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNG+AIP
Sbjct: 301 AIGSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIP 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKFNQATKELELVYLTGG+MGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFG 480
           QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTT+PKARIQFG
Sbjct: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540
           GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGL EDSRRSN
Sbjct: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSN 540

Query: 541 FTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           F+VMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV
Sbjct: 541 FSVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVN 720
           FTLNYPSM+VVFKHGTTSKMVSR LTNVGSPNSVYEVEVTAPEGVSV+VKP+RLVFKHVN
Sbjct: 661 FTLNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVN 720

Query: 721 ESLSYK 727
           ESLSYK
Sbjct: 721 ESLSYK 724

BLAST of Carg16904 vs. NCBI nr
Match: XP_008447369.1 (PREDICTED: subtilisin-like protease SBT1.2 [Cucumis melo])

HSP 1 Score: 1276.9 bits (3303), Expect = 0.0e+00
Identity = 641/726 (88.29%), Postives = 671/726 (92.42%), Query Frame = 0

Query: 1   MDFNSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDFN++M F  +FL FL L VQ N S LQTY+IQLHP GL +SVF+SKLQWHLSFLEQTL
Sbjct: 1   MDFNTRMGF-LLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLG 120
           S EED SSRLLYSYSNAMEGFAAQL+ETELEYLKKL DVVAVR DRKY+IQTTYSHKFLG
Sbjct: 61  SAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLG 120

Query: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
           LSV TQGV  KSSMGQGAIVG+LDTGVWPESPSFSDSKMPPIPQKWRG CQEGQDFNSS+
Sbjct: 121 LSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSN 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNG 240
           CNRKLIGAKFFIKGHHVASS PSD+VQEYVSPRDSHGHGT                  NG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAI DGVDILSLSLGGFPLPFFDDSI
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 360
           AIGSFRAMQHGISV+CAAGNNGPTQSSVANVAPWITTIGAGTLDR FPAIVRLSNGEAI 
Sbjct: 301 AIGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIY 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKF QATKELE+VYLTGG+MG ELCLKGSLPRE+V+GKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFG 480
           QIVKESGGAAMILAN+EINLEEDLVDVHVLPATLIGFAEANRLKAYINTTT+PKARIQFG
Sbjct: 421 QIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540
           GTVIGR+RAPSVAQFSSRGPS +NPSTLKPDVIAPGVNIIAAWPQNL PT L EDSRRSN
Sbjct: 481 GTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSN 540

Query: 541 FTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           FTVMSGTSM+CPHVSGI ALIHSAHPKWTPAAIKSAIMTTADVTD FGK ILDGNKPA V
Sbjct: 541 FTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPT+AIDPGLVYDIKPY+Y+IHLC LGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVN 720
           FTLNYPSMSV+FKHGTTSKMVSR LTNVGS NS+YEV+VTAPEGV V+VKPRRLVFKHVN
Sbjct: 661 FTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVN 720

Query: 721 ESLSYK 727
           +SL+YK
Sbjct: 721 QSLNYK 725

BLAST of Carg16904 vs. NCBI nr
Match: XP_011651620.1 (PREDICTED: subtilisin-like protease SBT1.2 [Cucumis sativus] >KGN58306.1 hypothetical protein Csa_3G610820 [Cucumis sativus])

HSP 1 Score: 1275.8 bits (3300), Expect = 0.0e+00
Identity = 636/726 (87.60%), Postives = 672/726 (92.56%), Query Frame = 0

Query: 1   MDFNSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDFN++M F  +FLCFL L VQ N S LQTY+IQLHP GL +SVF+SKLQWHLSFLEQ+L
Sbjct: 1   MDFNARMGF-LLFLCFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQSL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLG 120
           S EED SSRLLYSYSNAMEGFAAQLSETELEYLK+L DVVAVR DRKY+IQTTYSHKFLG
Sbjct: 61  SAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLKRLPDVVAVREDRKYQIQTTYSHKFLG 120

Query: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
           LSV TQG+  KSSMGQGAIVGVLDTGVWPESPSFSDSKMPP+PQKWRG CQEGQDFNSS+
Sbjct: 121 LSVGTQGLRQKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPVPQKWRGACQEGQDFNSSN 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNG 240
           CNRKLIGAKFFIKGHHVASS PSD+ QEYVSPRDSHGHGT                  NG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSLPSDVAQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 360
           AIGSFRAMQHGISV+CAAGNNGP QSSVANVAPWITTIGAGTLDR FPAI+RLSNGEAI 
Sbjct: 301 AIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGAGTLDRRFPAIIRLSNGEAIY 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKF QATKELE+VYLTGG+MG ELCLKGSLPRE+V+GKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFG 480
           QIVKESGGAAMILAN+EINLEEDLVDVHVLPATLIGFAEANRLKAYINTT++PKARIQFG
Sbjct: 421 QIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTSNPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540
           GTVIGR+RAPSVAQFSSRGPS +NPSTLKPDVIAPGVNIIAAWPQNL PTGL EDSRRSN
Sbjct: 481 GTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSN 540

Query: 541 FTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           FTVMSGTSM+CPHVSGI ALIHSAHPKWTPAAIKSAIMTTADVTD FGK ILDGNKPA V
Sbjct: 541 FTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPT+AIDPGLVYDIKPY+Y+IHLC LGYTHSEIFIITHMNVSCHK+LQMNKG
Sbjct: 601 FAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKILQMNKG 660

Query: 661 FTLNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVN 720
           FTLNYPS+SV+FKHGTTSKMVSR LTNVGS NS+YEV+VTAPEGV V+VKPRRLVFKHVN
Sbjct: 661 FTLNYPSISVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVN 720

Query: 721 ESLSYK 727
           +SL+YK
Sbjct: 721 QSLNYK 725

BLAST of Carg16904 vs. TAIR10
Match: AT1G04110.1 (Subtilase family protein)

HSP 1 Score: 969.9 bits (2506), Expect = 8.6e-283
Identity = 477/724 (65.88%), Postives = 581/724 (80.25%), Query Frame = 0

Query: 13  FLCFLLLQVQANASNL---QTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL----SVEED 72
           FLC + L   +++S +   QTY++QLHP   T+  F SK  WHLSFL++ +      EE+
Sbjct: 7   FLCIIFLLFCSSSSEILQKQTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEE 66

Query: 73  CSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLGL-SVC 132
            SSRLLYSY +A+EGFAAQL+E+E E L+   +VVAVR D   ++QTTYS+KFLGL    
Sbjct: 67  PSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFG 126

Query: 133 TQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRK 192
             GV  KS  GQG I+GVLDTGVWPESPSF D+ MP IP+KW+G CQEG+ F+SSSCNRK
Sbjct: 127 NSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRK 186

Query: 193 LIGAKFFIKGHHVASSPPS--DIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNGAG 252
           LIGA+FFI+GH VA+SP    ++ +EY+S RDS GHGT                  NGAG
Sbjct: 187 LIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAG 246

Query: 253 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAI 312
           VA+GMAPGAHIAVYKVCWF+GCYSSDI+AA+D AI+D VD+LSLSLGGFP+P +DD+IAI
Sbjct: 247 VARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAI 306

Query: 313 GSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIPGE 372
           G+FRAM+ GISVICAAGNNGP +SSVAN APW++TIGAGTLDR FPA+VRL+NG+ + GE
Sbjct: 307 GTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGE 366

Query: 373 SMYPGNKFNQATKELELVYLTGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQI 432
           S+YPG     A +E+E++Y+TGG  GSE CL+GSLPRE++ GKMV+CDRGVNGRSEKG+ 
Sbjct: 367 SLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVNGRSEKGEA 426

Query: 433 VKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFGGT 492
           VKE+GG AMILANTEIN EED +DVH+LPATLIG+ E+  LKAY+N T  PKARI FGGT
Sbjct: 427 VKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGT 486

Query: 493 VIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSNFT 552
           VIGR+RAP VAQFS+RGPS  NPS LKPD+IAPGVNIIAAWPQNL PTGL  DSRR NFT
Sbjct: 487 VIGRSRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFT 546

Query: 553 VMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASVFA 612
           VMSGTSMSCPHVSGI ALI SA+P W+PAAIKSA+MTTAD+ DR GK I DGNKPA VFA
Sbjct: 547 VMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGNKPAGVFA 606

Query: 613 MGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFT 672
           +GAGHVNP +AI+PGLVY+I+P DY+ +LC LG+T S+I  ITH NVSC+ +L+ N GF+
Sbjct: 607 IGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFS 666

Query: 673 LNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVNES 727
           LNYPS++V+FK G T++M++R +TNVGSPNS+Y V V APEG+ V V P+RLVFKHV+++
Sbjct: 667 LNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQT 726

BLAST of Carg16904 vs. TAIR10
Match: AT2G05920.1 (Subtilase family protein)

HSP 1 Score: 609.4 bits (1570), Expect = 2.9e-174
Identity = 333/700 (47.57%), Postives = 439/700 (62.71%), Query Frame = 0

Query: 30  TYMIQLHPQGLTSSVFESKLQWHLSFLEQTLSVEEDCSSRLLYSYSNAMEGFAAQLSETE 89
           TY+I+++      S F +   W+ S L        +  S LLY+Y+ +  GF+A L  TE
Sbjct: 29  TYIIRVNHSDKPES-FLTHHDWYTSQL--------NSESSLLYTYTTSFHGFSAYLDSTE 88

Query: 90  LEYLKKLAD-VVAVRADRKYEIQTTYSHKFLGLSVCTQGVCYKSSMGQGAIVGVLDTGVW 149
            + L   ++ ++ +  D  Y + TT + +FLGL+    GV    S   G I+GVLDTGVW
Sbjct: 89  ADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN-SEFGVHDLGSSSNGVIIGVLDTGVW 148

Query: 150 PESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRKLIGAKFFIKGHHVASSPPSDIVQE 209
           PES SF D+ MP IP KW+G C+ G DF+S  CN+KLIGA+ F KG  +AS       +E
Sbjct: 149 PESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRE 208

Query: 210 YVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNGAGVAQGMAPGAHIAVYKVCWFSGCYSSD 269
            VSPRD  GHGT                    AG A+GMA  A +A YKVCW +GC+ SD
Sbjct: 209 SVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSD 268

Query: 270 IVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQHGISVICAAGNNGPTQSSV 329
           I+AAMD AI DGVD+LSLSLGG   P++ D+IAIG+F AM+ G+ V C+AGN+GPT++SV
Sbjct: 269 ILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 328

Query: 330 ANVAPWITTIGAGTLDRTFPAIVRLSNGEAIPGESMYPGNKFNQATKELELVYLTGGKMG 389
           ANVAPW+ T+GAGTLDR FPA   L NG+ + G S+Y G      TK LELVY  G    
Sbjct: 329 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG--VGMGTKPLELVYNKGNSSS 388

Query: 390 SELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQIVKESGGAAMILANTEINLEEDLVDVH 449
           S LCL GSL    V GK+VVCDRGVN R EKG +V+++GG  MI+ANT  + EE + D H
Sbjct: 389 SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSH 448

Query: 450 VLPATLIGFAEANRLKAYINTTTDPKARIQFGGTVIGRTRAPSVAQFSSRGPSPTNPSTL 509
           +LPA  +G    + L+ Y+ + + P A + F GTV+    +P VA FSSRGP+   P  L
Sbjct: 449 LLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEIL 508

Query: 510 KPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSNFTVMSGTSMSCPHVSGIAALIHSAHPKW 569
           KPDVI PGVNI+A W   + PTGL +DSRR+ F +MSGTSMSCPH+SG+A L+ +AHP+W
Sbjct: 509 KPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEW 568

Query: 570 TPAAIKSAIMTTADVTDRFGKPILDG--NKPASVFAMGAGHVNPTRAIDPGLVYDIKPYD 629
           +P+AIKSA+MTTA V D    P+ D   N  ++ +A G+GHV+P +A+ PGLVYDI   +
Sbjct: 569 SPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEE 628

Query: 630 YVIHLCVLGYTHSEIF-IITHMNVSCHKVLQMNKGFTLNYPSMSVVFKHGTTSKMVSRWL 689
           Y+  LC L YT   I  I+   +V+C K  + +    LNYPS SV+F  G      +R +
Sbjct: 629 YIRFLCSLDYTVDHIVAIVKRPSVNCSK--KFSDPGQLNYPSFSVLF-GGKRVVRYTREV 688

Query: 690 TNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVNESLSY 726
           TNVG+ +SVY+V V     V + VKP +L FK V E   Y
Sbjct: 689 TNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRY 713

BLAST of Carg16904 vs. TAIR10
Match: AT3G14240.1 (Subtilase family protein)

HSP 1 Score: 599.0 bits (1543), Expect = 4.0e-171
Identity = 318/712 (44.66%), Postives = 444/712 (62.36%), Query Frame = 0

Query: 30  TYMIQLHPQGLTSSVFESKLQWHLSFLEQTLSVEEDCSSRLLYSYSNAMEGFAAQLSETE 89
           TY++ +  +    S+F +   W+ S L    S        ++++Y     GF+A+L+  +
Sbjct: 27  TYIVHVDHEA-KPSIFPTHFHWYTSSLASLTS----SPPSIIHTYDTVFHGFSARLTSQD 86

Query: 90  LEYLKKLADVVAVRADRKYEIQTTYSHKFLGL-SVCTQGVCYKSSMGQGAIVGVLDTGVW 149
              L     V++V  ++   + TT S +FLGL S    G+  +S  G   ++GV+DTGVW
Sbjct: 87  ASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVW 146

Query: 150 PESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRKLIGAKFFIKGHHVASSPPSDIVQE 209
           PE PSF D  + P+P KW+G C   QDF  S+CNRKL+GA+FF  G+   +   ++   E
Sbjct: 147 PERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNE-TTE 206

Query: 210 YVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNGAGVAQGMAPGAHIAVYKVCWFSGCYSSD 269
           + SPRDS GHGT                     GVA GMAP A +A YKVCW SGCY SD
Sbjct: 207 FRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSD 266

Query: 270 IVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQHGISVICAAGNNGPTQSSV 329
           I+AA D+A+ DGVD++SLS+GG  +P++ D+IAIG+F A+  GI V  +AGN GP   +V
Sbjct: 267 ILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTV 326

Query: 330 ANVAPWITTIGAGTLDRTFPAIVRLSNGEAIPGESMYPGNKFNQATKELELVY----LTG 389
            NVAPW+TT+GAGT+DR FPA V+L NG+ I G S+Y G   +   +   LVY    L G
Sbjct: 327 TNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPG-RMYPLVYGGSLLGG 386

Query: 390 GKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQIVKESGGAAMILANTEINLEEDL 449
               S LCL+GSL    V+GK+V+CDRG+N R+ KG+IV+++GG  MI+AN   + E  +
Sbjct: 387 DGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLV 446

Query: 450 VDVHVLPATLIGFAEANRLKAYIN------TTTDPKARIQFGGTVIGRTRAPSVAQFSSR 509
            D HVLPAT +G +  + ++ YI+      ++  P A I F GT +G   AP VA FS+R
Sbjct: 447 ADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSAR 506

Query: 510 GPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSNFTVMSGTSMSCPHVSGIA 569
           GP+P  P  LKPDVIAPG+NI+AAWP  + P+G+  D+RR+ F ++SGTSM+CPHVSG+A
Sbjct: 507 GPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLA 566

Query: 570 ALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGN--KPASVFAMGAGHVNPTRAIDP 629
           AL+ +AHP W+PAAI+SA++TTA   D  G+P++D +    +SV   G+GHV+PT+A+DP
Sbjct: 567 ALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDP 626

Query: 630 GLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFTLNYPSMSVVFKHGT 689
           GLVYDI  YDY+  LC   YT + I  IT     C    +      LNYPS SVVF+   
Sbjct: 627 GLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYG 686

Query: 690 TSKMVS---RWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVNESLSY 726
            SKM +   R +TNVG  +SVYE+++  P G +V V+P +L F+ V + LS+
Sbjct: 687 ESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSF 731

BLAST of Carg16904 vs. TAIR10
Match: AT5G67360.1 (Subtilase family protein)

HSP 1 Score: 597.8 bits (1540), Expect = 8.8e-171
Identity = 331/730 (45.34%), Postives = 454/730 (62.19%), Query Frame = 0

Query: 4   NSQMVFSTIF---LCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 63
           +S  + ST F   LC     V +++S+  TY++ +    + SS F+    W+ S L    
Sbjct: 2   SSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSS-FDLHSNWYDSSLR--- 61

Query: 64  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLG 123
           S+ +  S+ LLY+Y NA+ GF+ +L++ E + L     V++V  + +YE+ TT +  FLG
Sbjct: 62  SISD--SAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLG 121

Query: 124 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 183
           L   T  +  ++      +VGVLDTGVWPES S+SD    PIP  W+GGC+ G +F +S 
Sbjct: 122 LDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASL 181

Query: 184 CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNG 243
           CNRKLIGA+FF +G+  ++  P D  +E  SPRD  GHGT                    
Sbjct: 182 CNRKLIGARFFARGYE-STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 241

Query: 244 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 303
           +G A+GMAP A +AVYKVCW  GC+SSDI+AA+D AI D V++LS+SLGG    ++ D +
Sbjct: 242 SGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGV 301

Query: 304 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 363
           AIG+F AM+ GI V C+AGN GP+ SS++NVAPWITT+GAGTLDR FPA+  L NG+   
Sbjct: 302 AIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFT 361

Query: 364 GESMYPGNKFNQATKELELVYL--TGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSE 423
           G S++ G       K L  +Y           LC+ G+L  E+V+GK+V+CDRG+N R +
Sbjct: 362 GVSLFKGEAL--PDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQ 421

Query: 424 KGQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQ 483
           KG +VK +GG  MILANT  N EE + D H+LPAT +G    + ++ Y+ T  +P A I 
Sbjct: 422 KGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASIS 481

Query: 484 FGGTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRR 543
             GTV+G   +P VA FSSRGP+   P+ LKPD+IAPGVNI+AAW     PTGL  DSRR
Sbjct: 482 ILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRR 541

Query: 544 SNFTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILD--GNK 603
             F ++SGTSMSCPHVSG+AAL+ S HP+W+PAAI+SA+MTTA  T + GKP+LD    K
Sbjct: 542 VEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK 601

Query: 604 PASVFAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQ 663
           P++ F  GAGHV+PT A +PGL+YD+   DY+  LC L YT  +I  ++  N +C     
Sbjct: 602 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS 661

Query: 664 MNKGFTLNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTA-PEGVSVQVKPRRLV 723
            +    LNYPS +V    G  +   +R +T+VG   + Y V+VT+   GV + V+P  L 
Sbjct: 662 YSVA-DLNYPSFAVNV-DGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVLN 719

Query: 724 FKHVNESLSY 726
           FK  NE  SY
Sbjct: 722 FKEANEKKSY 719

BLAST of Carg16904 vs. TAIR10
Match: AT5G51750.1 (subtilase 1.3)

HSP 1 Score: 573.2 bits (1476), Expect = 2.3e-163
Identity = 320/731 (43.78%), Postives = 437/731 (59.78%), Query Frame = 0

Query: 8   VFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFL----EQTLSVE 67
           +  +I L FL  +     S  +TY+I +    +    + + LQW+ S +    +     E
Sbjct: 14  IILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLP-YTNHLQWYSSKINSVTQHKSQEE 73

Query: 68  EDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLGLS- 127
           E  ++R+LY+Y  A  G AAQL++ E E L++   VVAV  + +YE+ TT S  FLGL  
Sbjct: 74  EGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLER 133

Query: 128 VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCN 187
             ++ V  +       +VGVLDTG+WPES SF+D+ M P+P  WRG C+ G+ F   +CN
Sbjct: 134 QESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCN 193

Query: 188 RKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNGAG 247
           RK++GA+ F +G+  A+    D   EY SPRD  GHGT                     G
Sbjct: 194 RKIVGARVFYRGYEAATG-KIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 253

Query: 248 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAI 307
            A+GMA  A +A YKVCW  GC+SSDI++A+D A+ DGV +LS+SLGG    +  DS++I
Sbjct: 254 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 313

Query: 308 GSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIPGE 367
            +F AM+ G+ V C+AGN GP   S+ NV+PWITT+GA T+DR FPA V++       G 
Sbjct: 314 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 373

Query: 368 SMYPGNKFNQATKELELVYL---TGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEK 427
           S+Y G       K+  LVYL         +  CL G+L R  V GK+V+CDRGV  R +K
Sbjct: 374 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 433

Query: 428 GQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQF 487
           GQ+VK +GG  M+L NT  N EE + D H+LPA  +G  E   +K Y  T+    A ++ 
Sbjct: 434 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEI 493

Query: 488 GGTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRS 547
            GT IG   +P VA FSSRGP+  +   LKPD++APGVNI+AAW  ++AP+ L  D RR 
Sbjct: 494 LGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRV 553

Query: 548 NFTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILD--GNKP 607
            F ++SGTSMSCPHVSG+AALI S HP W+PAAIKSA+MTTA V D   KP+ D  G  P
Sbjct: 554 KFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAP 613

Query: 608 ASVFAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIIT-HMNVSCHKVLQ 667
           +S +  GAGH++P RA DPGLVYDI P +Y   LC    + S++ + T H N +C   L 
Sbjct: 614 SSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLA 673

Query: 668 MNKGFTLNYPSMSVVFKHGTTSKMVS--RWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRL 726
            N G  LNYP++S +F   T  K ++  R +TNVG   S Y+V V+  +G SV V+P+ L
Sbjct: 674 KNPG-NLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTL 733

BLAST of Carg16904 vs. Swiss-Prot
Match: sp|O64495|SBT12_ARATH (Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana OX=3702 GN=SBT1.2 PE=2 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 1.5e-281
Identity = 477/724 (65.88%), Postives = 581/724 (80.25%), Query Frame = 0

Query: 13  FLCFLLLQVQANASNL---QTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL----SVEED 72
           FLC + L   +++S +   QTY++QLHP   T+  F SK  WHLSFL++ +      EE+
Sbjct: 7   FLCIIFLLFCSSSSEILQKQTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEE 66

Query: 73  CSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLGL-SVC 132
            SSRLLYSY +A+EGFAAQL+E+E E L+   +VVAVR D   ++QTTYS+KFLGL    
Sbjct: 67  PSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFG 126

Query: 133 TQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRK 192
             GV  KS  GQG I+GVLDTGVWPESPSF D+ MP IP+KW+G CQEG+ F+SSSCNRK
Sbjct: 127 NSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRK 186

Query: 193 LIGAKFFIKGHHVASSPPS--DIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNGAG 252
           LIGA+FFI+GH VA+SP    ++ +EY+S RDS GHGT                  NGAG
Sbjct: 187 LIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAG 246

Query: 253 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAI 312
           VA+GMAPGAHIAVYKVCWF+GCYSSDI+AA+D AI+D VD+LSLSLGGFP+P +DD+IAI
Sbjct: 247 VARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAI 306

Query: 313 GSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIPGE 372
           G+FRAM+ GISVICAAGNNGP +SSVAN APW++TIGAGTLDR FPA+VRL+NG+ + GE
Sbjct: 307 GTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGE 366

Query: 373 SMYPGNKFNQATKELELVYLTGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQI 432
           S+YPG     A +E+E++Y+TGG  GSE CL+GSLPRE++ GKMV+CDRGVNGRSEKG+ 
Sbjct: 367 SLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVNGRSEKGEA 426

Query: 433 VKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFGGT 492
           VKE+GG AMILANTEIN EED +DVH+LPATLIG+ E+  LKAY+N T  PKARI FGGT
Sbjct: 427 VKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGT 486

Query: 493 VIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSNFT 552
           VIGR+RAP VAQFS+RGPS  NPS LKPD+IAPGVNIIAAWPQNL PTGL  DSRR NFT
Sbjct: 487 VIGRSRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFT 546

Query: 553 VMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASVFA 612
           VMSGTSMSCPHVSGI ALI SA+P W+PAAIKSA+MTTAD+ DR GK I DGNKPA VFA
Sbjct: 547 VMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGNKPAGVFA 606

Query: 613 MGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFT 672
           +GAGHVNP +AI+PGLVY+I+P DY+ +LC LG+T S+I  ITH NVSC+ +L+ N GF+
Sbjct: 607 IGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFS 666

Query: 673 LNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVNES 727
           LNYPS++V+FK G T++M++R +TNVGSPNS+Y V V APEG+ V V P+RLVFKHV+++
Sbjct: 667 LNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQT 726

BLAST of Carg16904 vs. Swiss-Prot
Match: sp|Q9ZUF6|SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 5.3e-173
Identity = 333/700 (47.57%), Postives = 439/700 (62.71%), Query Frame = 0

Query: 30  TYMIQLHPQGLTSSVFESKLQWHLSFLEQTLSVEEDCSSRLLYSYSNAMEGFAAQLSETE 89
           TY+I+++      S F +   W+ S L        +  S LLY+Y+ +  GF+A L  TE
Sbjct: 29  TYIIRVNHSDKPES-FLTHHDWYTSQL--------NSESSLLYTYTTSFHGFSAYLDSTE 88

Query: 90  LEYLKKLAD-VVAVRADRKYEIQTTYSHKFLGLSVCTQGVCYKSSMGQGAIVGVLDTGVW 149
            + L   ++ ++ +  D  Y + TT + +FLGL+    GV    S   G I+GVLDTGVW
Sbjct: 89  ADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLN-SEFGVHDLGSSSNGVIIGVLDTGVW 148

Query: 150 PESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRKLIGAKFFIKGHHVASSPPSDIVQE 209
           PES SF D+ MP IP KW+G C+ G DF+S  CN+KLIGA+ F KG  +AS       +E
Sbjct: 149 PESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRE 208

Query: 210 YVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNGAGVAQGMAPGAHIAVYKVCWFSGCYSSD 269
            VSPRD  GHGT                    AG A+GMA  A +A YKVCW +GC+ SD
Sbjct: 209 SVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSD 268

Query: 270 IVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQHGISVICAAGNNGPTQSSV 329
           I+AAMD AI DGVD+LSLSLGG   P++ D+IAIG+F AM+ G+ V C+AGN+GPT++SV
Sbjct: 269 ILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 328

Query: 330 ANVAPWITTIGAGTLDRTFPAIVRLSNGEAIPGESMYPGNKFNQATKELELVYLTGGKMG 389
           ANVAPW+ T+GAGTLDR FPA   L NG+ + G S+Y G      TK LELVY  G    
Sbjct: 329 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG--VGMGTKPLELVYNKGNSSS 388

Query: 390 SELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQIVKESGGAAMILANTEINLEEDLVDVH 449
           S LCL GSL    V GK+VVCDRGVN R EKG +V+++GG  MI+ANT  + EE + D H
Sbjct: 389 SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSH 448

Query: 450 VLPATLIGFAEANRLKAYINTTTDPKARIQFGGTVIGRTRAPSVAQFSSRGPSPTNPSTL 509
           +LPA  +G    + L+ Y+ + + P A + F GTV+    +P VA FSSRGP+   P  L
Sbjct: 449 LLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEIL 508

Query: 510 KPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSNFTVMSGTSMSCPHVSGIAALIHSAHPKW 569
           KPDVI PGVNI+A W   + PTGL +DSRR+ F +MSGTSMSCPH+SG+A L+ +AHP+W
Sbjct: 509 KPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEW 568

Query: 570 TPAAIKSAIMTTADVTDRFGKPILDG--NKPASVFAMGAGHVNPTRAIDPGLVYDIKPYD 629
           +P+AIKSA+MTTA V D    P+ D   N  ++ +A G+GHV+P +A+ PGLVYDI   +
Sbjct: 569 SPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEE 628

Query: 630 YVIHLCVLGYTHSEIF-IITHMNVSCHKVLQMNKGFTLNYPSMSVVFKHGTTSKMVSRWL 689
           Y+  LC L YT   I  I+   +V+C K  + +    LNYPS SV+F  G      +R +
Sbjct: 629 YIRFLCSLDYTVDHIVAIVKRPSVNCSK--KFSDPGQLNYPSFSVLF-GGKRVVRYTREV 688

Query: 690 TNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVNESLSY 726
           TNVG+ +SVY+V V     V + VKP +L FK V E   Y
Sbjct: 689 TNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRY 713

BLAST of Carg16904 vs. Swiss-Prot
Match: sp|Q9LUM3|SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 7.1e-170
Identity = 318/712 (44.66%), Postives = 444/712 (62.36%), Query Frame = 0

Query: 30  TYMIQLHPQGLTSSVFESKLQWHLSFLEQTLSVEEDCSSRLLYSYSNAMEGFAAQLSETE 89
           TY++ +  +    S+F +   W+ S L    S        ++++Y     GF+A+L+  +
Sbjct: 27  TYIVHVDHEA-KPSIFPTHFHWYTSSLASLTS----SPPSIIHTYDTVFHGFSARLTSQD 86

Query: 90  LEYLKKLADVVAVRADRKYEIQTTYSHKFLGL-SVCTQGVCYKSSMGQGAIVGVLDTGVW 149
              L     V++V  ++   + TT S +FLGL S    G+  +S  G   ++GV+DTGVW
Sbjct: 87  ASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVW 146

Query: 150 PESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRKLIGAKFFIKGHHVASSPPSDIVQE 209
           PE PSF D  + P+P KW+G C   QDF  S+CNRKL+GA+FF  G+   +   ++   E
Sbjct: 147 PERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNE-TTE 206

Query: 210 YVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNGAGVAQGMAPGAHIAVYKVCWFSGCYSSD 269
           + SPRDS GHGT                     GVA GMAP A +A YKVCW SGCY SD
Sbjct: 207 FRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSD 266

Query: 270 IVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQHGISVICAAGNNGPTQSSV 329
           I+AA D+A+ DGVD++SLS+GG  +P++ D+IAIG+F A+  GI V  +AGN GP   +V
Sbjct: 267 ILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTV 326

Query: 330 ANVAPWITTIGAGTLDRTFPAIVRLSNGEAIPGESMYPGNKFNQATKELELVY----LTG 389
            NVAPW+TT+GAGT+DR FPA V+L NG+ I G S+Y G   +   +   LVY    L G
Sbjct: 327 TNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPG-RMYPLVYGGSLLGG 386

Query: 390 GKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQIVKESGGAAMILANTEINLEEDL 449
               S LCL+GSL    V+GK+V+CDRG+N R+ KG+IV+++GG  MI+AN   + E  +
Sbjct: 387 DGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLV 446

Query: 450 VDVHVLPATLIGFAEANRLKAYIN------TTTDPKARIQFGGTVIGRTRAPSVAQFSSR 509
            D HVLPAT +G +  + ++ YI+      ++  P A I F GT +G   AP VA FS+R
Sbjct: 447 ADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSAR 506

Query: 510 GPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSNFTVMSGTSMSCPHVSGIA 569
           GP+P  P  LKPDVIAPG+NI+AAWP  + P+G+  D+RR+ F ++SGTSM+CPHVSG+A
Sbjct: 507 GPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLA 566

Query: 570 ALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGN--KPASVFAMGAGHVNPTRAIDP 629
           AL+ +AHP W+PAAI+SA++TTA   D  G+P++D +    +SV   G+GHV+PT+A+DP
Sbjct: 567 ALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDP 626

Query: 630 GLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFTLNYPSMSVVFKHGT 689
           GLVYDI  YDY+  LC   YT + I  IT     C    +      LNYPS SVVF+   
Sbjct: 627 GLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYG 686

Query: 690 TSKMVS---RWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVNESLSY 726
            SKM +   R +TNVG  +SVYE+++  P G +V V+P +L F+ V + LS+
Sbjct: 687 ESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSF 731

BLAST of Carg16904 vs. Swiss-Prot
Match: sp|O65351|SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.6e-169
Identity = 331/730 (45.34%), Postives = 454/730 (62.19%), Query Frame = 0

Query: 4   NSQMVFSTIF---LCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 63
           +S  + ST F   LC     V +++S+  TY++ +    + SS F+    W+ S L    
Sbjct: 2   SSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSS-FDLHSNWYDSSLR--- 61

Query: 64  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLG 123
           S+ +  S+ LLY+Y NA+ GF+ +L++ E + L     V++V  + +YE+ TT +  FLG
Sbjct: 62  SISD--SAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLG 121

Query: 124 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 183
           L   T  +  ++      +VGVLDTGVWPES S+SD    PIP  W+GGC+ G +F +S 
Sbjct: 122 LDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASL 181

Query: 184 CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNG 243
           CNRKLIGA+FF +G+  ++  P D  +E  SPRD  GHGT                    
Sbjct: 182 CNRKLIGARFFARGYE-STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 241

Query: 244 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 303
           +G A+GMAP A +AVYKVCW  GC+SSDI+AA+D AI D V++LS+SLGG    ++ D +
Sbjct: 242 SGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGV 301

Query: 304 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 363
           AIG+F AM+ GI V C+AGN GP+ SS++NVAPWITT+GAGTLDR FPA+  L NG+   
Sbjct: 302 AIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFT 361

Query: 364 GESMYPGNKFNQATKELELVYL--TGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSE 423
           G S++ G       K L  +Y           LC+ G+L  E+V+GK+V+CDRG+N R +
Sbjct: 362 GVSLFKGEAL--PDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQ 421

Query: 424 KGQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQ 483
           KG +VK +GG  MILANT  N EE + D H+LPAT +G    + ++ Y+ T  +P A I 
Sbjct: 422 KGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASIS 481

Query: 484 FGGTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRR 543
             GTV+G   +P VA FSSRGP+   P+ LKPD+IAPGVNI+AAW     PTGL  DSRR
Sbjct: 482 ILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRR 541

Query: 544 SNFTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILD--GNK 603
             F ++SGTSMSCPHVSG+AAL+ S HP+W+PAAI+SA+MTTA  T + GKP+LD    K
Sbjct: 542 VEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK 601

Query: 604 PASVFAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQ 663
           P++ F  GAGHV+PT A +PGL+YD+   DY+  LC L YT  +I  ++  N +C     
Sbjct: 602 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS 661

Query: 664 MNKGFTLNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTA-PEGVSVQVKPRRLV 723
            +    LNYPS +V    G  +   +R +T+VG   + Y V+VT+   GV + V+P  L 
Sbjct: 662 YSVA-DLNYPSFAVNV-DGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVLN 719

Query: 724 FKHVNESLSY 726
           FK  NE  SY
Sbjct: 722 FKEANEKKSY 719

BLAST of Carg16904 vs. Swiss-Prot
Match: sp|Q9FLI4|SBT13_ARATH (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 4.2e-162
Identity = 320/731 (43.78%), Postives = 437/731 (59.78%), Query Frame = 0

Query: 8   VFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFL----EQTLSVE 67
           +  +I L FL  +     S  +TY+I +    +    + + LQW+ S +    +     E
Sbjct: 14  IILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLP-YTNHLQWYSSKINSVTQHKSQEE 73

Query: 68  EDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLGLS- 127
           E  ++R+LY+Y  A  G AAQL++ E E L++   VVAV  + +YE+ TT S  FLGL  
Sbjct: 74  EGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLER 133

Query: 128 VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCN 187
             ++ V  +       +VGVLDTG+WPES SF+D+ M P+P  WRG C+ G+ F   +CN
Sbjct: 134 QESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCN 193

Query: 188 RKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNGAG 247
           RK++GA+ F +G+  A+    D   EY SPRD  GHGT                     G
Sbjct: 194 RKIVGARVFYRGYEAATG-KIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 253

Query: 248 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAI 307
            A+GMA  A +A YKVCW  GC+SSDI++A+D A+ DGV +LS+SLGG    +  DS++I
Sbjct: 254 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 313

Query: 308 GSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIPGE 367
            +F AM+ G+ V C+AGN GP   S+ NV+PWITT+GA T+DR FPA V++       G 
Sbjct: 314 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 373

Query: 368 SMYPGNKFNQATKELELVYL---TGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEK 427
           S+Y G       K+  LVYL         +  CL G+L R  V GK+V+CDRGV  R +K
Sbjct: 374 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 433

Query: 428 GQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQF 487
           GQ+VK +GG  M+L NT  N EE + D H+LPA  +G  E   +K Y  T+    A ++ 
Sbjct: 434 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEI 493

Query: 488 GGTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRS 547
            GT IG   +P VA FSSRGP+  +   LKPD++APGVNI+AAW  ++AP+ L  D RR 
Sbjct: 494 LGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRV 553

Query: 548 NFTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILD--GNKP 607
            F ++SGTSMSCPHVSG+AALI S HP W+PAAIKSA+MTTA V D   KP+ D  G  P
Sbjct: 554 KFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAP 613

Query: 608 ASVFAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIIT-HMNVSCHKVLQ 667
           +S +  GAGH++P RA DPGLVYDI P +Y   LC    + S++ + T H N +C   L 
Sbjct: 614 SSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLA 673

Query: 668 MNKGFTLNYPSMSVVFKHGTTSKMVS--RWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRL 726
            N G  LNYP++S +F   T  K ++  R +TNVG   S Y+V V+  +G SV V+P+ L
Sbjct: 674 KNPG-NLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTL 733

BLAST of Carg16904 vs. TrEMBL
Match: tr|A0A1S3BHA2|A0A1S3BHA2_CUCME (subtilisin-like protease SBT1.2 OS=Cucumis melo OX=3656 GN=LOC103489836 PE=4 SV=1)

HSP 1 Score: 1276.9 bits (3303), Expect = 0.0e+00
Identity = 641/726 (88.29%), Postives = 671/726 (92.42%), Query Frame = 0

Query: 1   MDFNSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDFN++M F  +FL FL L VQ N S LQTY+IQLHP GL +SVF+SKLQWHLSFLEQTL
Sbjct: 1   MDFNTRMGF-LLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLG 120
           S EED SSRLLYSYSNAMEGFAAQL+ETELEYLKKL DVVAVR DRKY+IQTTYSHKFLG
Sbjct: 61  SAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLG 120

Query: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
           LSV TQGV  KSSMGQGAIVG+LDTGVWPESPSFSDSKMPPIPQKWRG CQEGQDFNSS+
Sbjct: 121 LSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSN 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNG 240
           CNRKLIGAKFFIKGHHVASS PSD+VQEYVSPRDSHGHGT                  NG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAI DGVDILSLSLGGFPLPFFDDSI
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 360
           AIGSFRAMQHGISV+CAAGNNGPTQSSVANVAPWITTIGAGTLDR FPAIVRLSNGEAI 
Sbjct: 301 AIGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIY 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKF QATKELE+VYLTGG+MG ELCLKGSLPRE+V+GKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFG 480
           QIVKESGGAAMILAN+EINLEEDLVDVHVLPATLIGFAEANRLKAYINTTT+PKARIQFG
Sbjct: 421 QIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540
           GTVIGR+RAPSVAQFSSRGPS +NPSTLKPDVIAPGVNIIAAWPQNL PT L EDSRRSN
Sbjct: 481 GTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSN 540

Query: 541 FTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           FTVMSGTSM+CPHVSGI ALIHSAHPKWTPAAIKSAIMTTADVTD FGK ILDGNKPA V
Sbjct: 541 FTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPT+AIDPGLVYDIKPY+Y+IHLC LGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVN 720
           FTLNYPSMSV+FKHGTTSKMVSR LTNVGS NS+YEV+VTAPEGV V+VKPRRLVFKHVN
Sbjct: 661 FTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVN 720

Query: 721 ESLSYK 727
           +SL+YK
Sbjct: 721 QSLNYK 725

BLAST of Carg16904 vs. TrEMBL
Match: tr|A0A0A0LDY7|A0A0A0LDY7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G610820 PE=4 SV=1)

HSP 1 Score: 1275.8 bits (3300), Expect = 0.0e+00
Identity = 636/726 (87.60%), Postives = 672/726 (92.56%), Query Frame = 0

Query: 1   MDFNSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDFN++M F  +FLCFL L VQ N S LQTY+IQLHP GL +SVF+SKLQWHLSFLEQ+L
Sbjct: 1   MDFNARMGF-LLFLCFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQSL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLG 120
           S EED SSRLLYSYSNAMEGFAAQLSETELEYLK+L DVVAVR DRKY+IQTTYSHKFLG
Sbjct: 61  SAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLKRLPDVVAVREDRKYQIQTTYSHKFLG 120

Query: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
           LSV TQG+  KSSMGQGAIVGVLDTGVWPESPSFSDSKMPP+PQKWRG CQEGQDFNSS+
Sbjct: 121 LSVGTQGLRQKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPVPQKWRGACQEGQDFNSSN 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXNG 240
           CNRKLIGAKFFIKGHHVASS PSD+ QEYVSPRDSHGHGT                  NG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSLPSDVAQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 360
           AIGSFRAMQHGISV+CAAGNNGP QSSVANVAPWITTIGAGTLDR FPAI+RLSNGEAI 
Sbjct: 301 AIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGAGTLDRRFPAIIRLSNGEAIY 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKF QATKELE+VYLTGG+MG ELCLKGSLPRE+V+GKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFG 480
           QIVKESGGAAMILAN+EINLEEDLVDVHVLPATLIGFAEANRLKAYINTT++PKARIQFG
Sbjct: 421 QIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTSNPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540
           GTVIGR+RAPSVAQFSSRGPS +NPSTLKPDVIAPGVNIIAAWPQNL PTGL EDSRRSN
Sbjct: 481 GTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSN 540

Query: 541 FTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           FTVMSGTSM+CPHVSGI ALIHSAHPKWTPAAIKSAIMTTADVTD FGK ILDGNKPA V
Sbjct: 541 FTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPT+AIDPGLVYDIKPY+Y+IHLC LGYTHSEIFIITHMNVSCHK+LQMNKG
Sbjct: 601 FAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKILQMNKG 660

Query: 661 FTLNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVN 720
           FTLNYPS+SV+FKHGTTSKMVSR LTNVGS NS+YEV+VTAPEGV V+VKPRRLVFKHVN
Sbjct: 661 FTLNYPSISVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVN 720

Query: 721 ESLSYK 727
           +SL+YK
Sbjct: 721 QSLNYK 725

BLAST of Carg16904 vs. TrEMBL
Match: tr|A0A2N9HDH2|A0A2N9HDH2_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS37602 PE=4 SV=1)

HSP 1 Score: 1073.9 bits (2776), Expect = 1.5e-310
Identity = 529/728 (72.66%), Postives = 608/728 (83.52%), Query Frame = 0

Query: 1   MDFNSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           M+  +Q+ FST+F   + L    NA+ LQTY++QLHP G+TSS+F S L WHLSFLE+T+
Sbjct: 1   MEAITQLFFSTLFASLVCL----NANTLQTYIVQLHPHGITSSLFNSNLHWHLSFLEKTV 60

Query: 61  -SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFL 120
            S EED S RLLYSY +AMEGFAAQLSE E+E LKKL DV+A+R DR+ +I TTYS+KFL
Sbjct: 61  SSKEEDPSHRLLYSYHSAMEGFAAQLSEAEVESLKKLPDVIAIRPDRRLQIHTTYSYKFL 120

Query: 121 GLSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSS 180
           GL+       YKS  G+G I+GVLDTGVWPESPSF D  MPP+P+KW G CQEGQ FNSS
Sbjct: 121 GLNTTRGNAWYKSGFGRGTIIGVLDTGVWPESPSFDDHGMPPVPKKWGGICQEGQSFNSS 180

Query: 181 SCNRKLIGAKFFIKGHHVAS-SPPSDIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXX 240
           +CNRKLIGA+FF +GH VAS S   +IVQEYVSPRDSHGHGT                  
Sbjct: 181 NCNRKLIGARFFTEGHRVASMSSSPNIVQEYVSPRDSHGHGTHTISTVGGALVPMASVFG 240

Query: 241 NGAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDD 300
           NGAGVA+GMAP  HIA+YKVCW++GCYSSDIVAAMD+AIRDGVDILSLSLGGFPLP ++D
Sbjct: 241 NGAGVARGMAPDTHIAMYKVCWYNGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPIYED 300

Query: 301 SIAIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEA 360
           SIAIGSFRAM+HGISVICAAGNNGP +SSV+N APWI+TIGA TLDR FPAIVR+ NGEA
Sbjct: 301 SIAIGSFRAMEHGISVICAAGNNGPIESSVSNEAPWISTIGASTLDRKFPAIVRIGNGEA 360

Query: 361 IPGESMYPGNKFNQATKELELVYLTGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSE 420
           + GESMYPGN F  A +EL+LVY+TGG  GSE CL+G+LPRE+V GKMV+CDRGVNGR E
Sbjct: 361 LYGESMYPGNHFMTAGRELKLVYVTGGNRGSEFCLQGTLPREKVRGKMVICDRGVNGRGE 420

Query: 421 KGQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQ 480
           KGQ+VKE+GGAAMIL NT INLEED VDVHVLPAT++GF E+ RLKAYIN+T  P ARI 
Sbjct: 421 KGQVVKEAGGAAMILTNTAINLEEDSVDVHVLPATMVGFDESVRLKAYINSTMKPMARIV 480

Query: 481 FGGTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRR 540
           FGGT IG++R P+VAQFS RGPS TNPS LKPDVIAPGVNIIAAWPQNL PTGL EDSRR
Sbjct: 481 FGGTEIGKSRGPAVAQFSGRGPSYTNPSILKPDVIAPGVNIIAAWPQNLGPTGLPEDSRR 540

Query: 541 SNFTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPA 600
            NFTVMSGTSM+CPHVSGIAALIHS HPKWTPAAIKSA+MTTAD+TD  GKPI+DG+KPA
Sbjct: 541 VNFTVMSGTSMACPHVSGIAALIHSVHPKWTPAAIKSALMTTADITDHSGKPIMDGDKPA 600

Query: 601 SVFAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMN 660
            VFA+GAGHVNP +AIDPGL+YDIKP +Y+ HLC LGYT  EIF ITH NVSCH+VLQMN
Sbjct: 601 GVFAIGAGHVNPGKAIDPGLIYDIKPDEYITHLCTLGYTIKEIFTITHRNVSCHEVLQMN 660

Query: 661 KGFTLNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKH 720
           +GF+LNYPS+SV+FKHG  SKM+ R LTNVGSPNS+Y VEVTAPEGV V+VKP+RL+FKH
Sbjct: 661 RGFSLNYPSISVIFKHGMRSKMIKRRLTNVGSPNSIYSVEVTAPEGVKVRVKPQRLIFKH 720

Query: 721 VNESLSYK 727
           +N+SLSYK
Sbjct: 721 INQSLSYK 724

BLAST of Carg16904 vs. TrEMBL
Match: tr|A0A061DNN4|A0A061DNN4_THECC (Subtilase family protein isoform 1 OS=Theobroma cacao OX=3641 GN=TCM_003899 PE=4 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 1.9e-308
Identity = 530/727 (72.90%), Postives = 610/727 (83.91%), Query Frame = 0

Query: 1   MDFNSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           M+   Q+  ST+FL   L+ V+AN   LQTY++QLHP G+TSS+F +KLQWHLSFLEQTL
Sbjct: 1   MEAKIQLFCSTLFL--YLVSVRAN--TLQTYIVQLHPHGVTSSLFPTKLQWHLSFLEQTL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLG 120
           S EED SSRLLYSY +AM+GFAAQLSETELE L+ L DVVA+R DR  +I TTYS+KFLG
Sbjct: 61  SSEEDPSSRLLYSYGSAMDGFAAQLSETELELLRSLPDVVAIRPDRLLQIHTTYSYKFLG 120

Query: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
           LS    G  +KS +G+G I+GVLDTGVWPESPSF+D  MPP+P+KWRG CQEGQ FN+ +
Sbjct: 121 LSTTRDGAWFKSGLGRGTIIGVLDTGVWPESPSFNDQGMPPVPKKWRGICQEGQSFNALN 180

Query: 181 CNRKLIGAKFFIKGHHVAS-SPPSDIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXN 240
           CNRKLIGA+FFIKGHHV+S S  ++++QEY+SPRDS GHGT                  N
Sbjct: 181 CNRKLIGARFFIKGHHVSSVSRSANMIQEYISPRDSSGHGTHTSSTAGGVSVPMASVLGN 240

Query: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVA+GMAPGAHIAVYKVCWF+GCYSSDI+AAMD AI DGVD+LSLSLGGFPLP FDDS
Sbjct: 241 GAGVARGMAPGAHIAVYKVCWFNGCYSSDILAAMDVAIADGVDVLSLSLGGFPLPLFDDS 300

Query: 301 IAIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAI 360
           IA+GSFRA++HGISVICAAGNNGP QSSVAN+APWI TIGA TLDR FPAIVR+ NGE I
Sbjct: 301 IAVGSFRAVEHGISVICAAGNNGPIQSSVANIAPWIATIGASTLDRKFPAIVRMGNGEFI 360

Query: 361 PGESMYPGNKFNQATKELELVYLTGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEK 420
            GES+YPGN+   A KELELVY+TGG  GSE C KGSLPR +V GKMV+CDRGVNGR+EK
Sbjct: 361 YGESVYPGNRLRSAKKELELVYVTGGDSGSEFCFKGSLPRAKVGGKMVICDRGVNGRAEK 420

Query: 421 GQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQF 480
           G  VKE+GGAAMILANTEINLEED VD HVLPAT IG+AEA RLKAYINTT+ P+ARI F
Sbjct: 421 GIAVKEAGGAAMILANTEINLEEDSVDAHVLPATEIGYAEAVRLKAYINTTSRPRARIIF 480

Query: 481 GGTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRS 540
           GGTVIGR+RAP+VA FS+RGP+  + S LKPDVIAPGVNIIAAWPQNL PTGL ED+RR 
Sbjct: 481 GGTVIGRSRAPTVALFSARGPNLYDSSILKPDVIAPGVNIIAAWPQNLGPTGLPEDARRV 540

Query: 541 NFTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPAS 600
           NFTVMSGTSM+CPHVSGIAALIHSAHP WTPAAIKSA+MT+AD  D  GKPI DGNKPA 
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPTWTPAAIKSALMTSADANDHRGKPITDGNKPAD 600

Query: 601 VFAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFA+GAGHVNP RAIDPGL+YDI+P +YVIHLC LGYT SEIF ITH NVSC ++L+MN+
Sbjct: 601 VFAIGAGHVNPDRAIDPGLIYDIRPDEYVIHLCTLGYTRSEIFTITHKNVSCSEILRMNR 660

Query: 661 GFTLNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHV 720
           GFTLNYPS++V FK G  SKM++R LTNVGSP+SVY VEV APEGV V+VKP+RL+F+H+
Sbjct: 661 GFTLNYPSITVAFKQGMKSKMITRRLTNVGSPHSVYSVEVKAPEGVKVKVKPQRLIFEHI 720

Query: 721 NESLSYK 727
           N+SLSY+
Sbjct: 721 NQSLSYR 723

BLAST of Carg16904 vs. TrEMBL
Match: tr|F6HZR3|F6HZR3_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_07s0005g04410 PE=4 SV=1)

HSP 1 Score: 1058.5 bits (2736), Expect = 6.7e-306
Identity = 525/727 (72.21%), Postives = 607/727 (83.49%), Query Frame = 0

Query: 1   MDFNSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           M+  +Q++FS +FL FL   V  +A +LQTY+IQLHP G T+S F SK+QWHLSFLE+ +
Sbjct: 1   MEAKAQLLFSVLFL-FL---VFVHAQSLQTYIIQLHPHGATASSFSSKVQWHLSFLERIM 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLG 120
             E+D SSRLLYSY +AMEGFAAQLSETELE L+KL +V+AVR D + ++ TTYS+KFLG
Sbjct: 61  FSEDDPSSRLLYSYHSAMEGFAAQLSETELESLRKLGEVIAVRPDTRLQLHTTYSYKFLG 120

Query: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
           LS  ++G  ++S  G G IVGVLDTGVWPESPSFSD  MPP+P+KWRG CQEGQDFNSS+
Sbjct: 121 LSPASRGGWFQSGFGHGTIVGVLDTGVWPESPSFSDHGMPPVPKKWRGVCQEGQDFNSSN 180

Query: 181 CNRKLIGAKFFIKGHHVAS-SPPSDIVQEYVSPRDSHGHGTXXXXXXXXXXXXXXXXXXN 240
           CNRKLIGA+FF KGH VAS SP SD V EYVS RDSHGHGT                  N
Sbjct: 181 CNRKLIGARFFSKGHRVASISPSSDTVVEYVSARDSHGHGTHTSSTAGGASVPMASVLGN 240

Query: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDS 300
           GAGVAQGMAP AHIA+YKVCWFSGCYSSDI+AAMD AIRDGVDILSLSLGGFP+P FDDS
Sbjct: 241 GAGVAQGMAPRAHIAIYKVCWFSGCYSSDILAAMDVAIRDGVDILSLSLGGFPIPLFDDS 300

Query: 301 IAIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAI 360
           IAIGSFRAM+HGISVICAAGNNGP QSSVAN APWITT+GA TLDR FPAIVR+ NG+ +
Sbjct: 301 IAIGSFRAMEHGISVICAAGNNGPIQSSVANEAPWITTVGASTLDRRFPAIVRMGNGKRL 360

Query: 361 PGESMYPGNKFNQATKELELVYLTGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEK 420
            GESMYPG     A KELELVY+TGG  GSE C KGSLPR +V GKMVVCDRGVNGR+EK
Sbjct: 361 YGESMYPGKHNPYAGKELELVYVTGGDSGSEFCFKGSLPRAKVLGKMVVCDRGVNGRAEK 420

Query: 421 GQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQF 480
           G+ VKE+GGAAMILANT+INLEED VD HVLPA+LIGFAE+ +LK+Y+N++  P ARI+F
Sbjct: 421 GEAVKEAGGAAMILANTDINLEEDSVDAHVLPASLIGFAESVQLKSYMNSSRTPTARIEF 480

Query: 481 GGTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRS 540
           GGTVIG++RAP+VAQFSSRGPS TNP+ LKPD+IAPGVNIIAAWPQNL P+GL EDSRR 
Sbjct: 481 GGTVIGKSRAPAVAQFSSRGPSLTNPTILKPDIIAPGVNIIAAWPQNLGPSGLPEDSRRV 540

Query: 541 NFTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPAS 600
           NFTVMSGTSM+CPH+SGIAALIHSA+P WTPAAIKSA++TTADVTD  GKPI+D NKPA 
Sbjct: 541 NFTVMSGTSMACPHISGIAALIHSANPTWTPAAIKSAMITTADVTDHTGKPIMDSNKPAG 600

Query: 601 VFAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNK 660
           VFAMGAG VNP +AIDPGL+YDIKP +Y+ HLC LGYT SEI  ITH NVSCH+++Q NK
Sbjct: 601 VFAMGAGQVNPEKAIDPGLIYDIKPDEYITHLCTLGYTRSEISAITHRNVSCHELVQKNK 660

Query: 661 GFTLNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHV 720
           GF+LNYPS+SV+F+HG  S+M+ R LTNVG PNS+Y VEV APEGV V+VKP  L+FKH+
Sbjct: 661 GFSLNYPSISVIFRHGMMSRMIKRRLTNVGVPNSIYSVEVVAPEGVKVRVKPHHLIFKHI 720

Query: 721 NESLSYK 727
           N+SLSY+
Sbjct: 721 NQSLSYR 723

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022963543.10.0e+0099.04subtilisin-like protease SBT1.2 [Cucurbita moschata][more]
XP_022967188.10.0e+0095.59subtilisin-like protease SBT1.2 [Cucurbita maxima][more]
XP_023553603.10.0e+0097.66subtilisin-like protease SBT1.2 [Cucurbita pepo subsp. pepo][more]
XP_008447369.10.0e+0088.29PREDICTED: subtilisin-like protease SBT1.2 [Cucumis melo][more]
XP_011651620.10.0e+0087.60PREDICTED: subtilisin-like protease SBT1.2 [Cucumis sativus] >KGN58306.1 hypothe... [more]
Match NameE-valueIdentityDescription
AT1G04110.18.6e-28365.88Subtilase family protein[more]
AT2G05920.12.9e-17447.57Subtilase family protein[more]
AT3G14240.14.0e-17144.66Subtilase family protein[more]
AT5G67360.18.8e-17145.34Subtilase family protein[more]
AT5G51750.12.3e-16343.78subtilase 1.3[more]
Match NameE-valueIdentityDescription
sp|O64495|SBT12_ARATH1.5e-28165.88Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana OX=3702 GN=SBT1.2 PE=2 S... [more]
sp|Q9ZUF6|SBT18_ARATH5.3e-17347.57Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
sp|Q9LUM3|SBT15_ARATH7.1e-17044.66Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
sp|O65351|SBT17_ARATH1.6e-16945.34Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
sp|Q9FLI4|SBT13_ARATH4.2e-16243.78Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3BHA2|A0A1S3BHA2_CUCME0.0e+0088.29subtilisin-like protease SBT1.2 OS=Cucumis melo OX=3656 GN=LOC103489836 PE=4 SV=... [more]
tr|A0A0A0LDY7|A0A0A0LDY7_CUCSA0.0e+0087.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G610820 PE=4 SV=1[more]
tr|A0A2N9HDH2|A0A2N9HDH2_FAGSY1.5e-31072.66Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS37602 PE=4 SV=1[more]
tr|A0A061DNN4|A0A061DNN4_THECC1.9e-30872.90Subtilase family protein isoform 1 OS=Theobroma cacao OX=3641 GN=TCM_003899 PE=4... [more]
tr|F6HZR3|F6HZR3_VITVI6.7e-30672.21Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_07s0005g04410 PE=4 SV=... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: INTERPRO
TermDefinition
IPR034197Peptidases_S8_3
IPR023828Peptidase_S8_Ser-AS
IPR003137PA_domain
IPR037045S8pro/Inhibitor_I9_sf
IPR010259S8pro/Inhibitor_I9
IPR000209Peptidase_S8/S53_dom
IPR036852Peptidase_S8/S53_dom_sf
IPR015500Peptidase_S8_subtilisin-rel
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16904-RACarg16904-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 135..154
score: 37.11
coord: 214..227
score: 44.04
coord: 545..561
score: 58.8
IPR036852Peptidase S8/S53 domain superfamilyGENE3DG3DSA:3.40.50.200coord: 485..628
e-value: 2.8E-180
score: 602.1
coord: 137..343
e-value: 2.8E-180
score: 602.1
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILYSSF52743Subtilisin-likecoord: 211..347
coord: 493..619
coord: 131..171
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 135..606
e-value: 5.3E-55
score: 186.7
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 30..110
e-value: 3.1E-13
score: 50.1
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3DG3DSA:3.30.70.80coord: 19..111
e-value: 2.2E-9
score: 39.6
NoneNo IPR availableGENE3DG3DSA:3.50.30.30coord: 344..484
e-value: 2.8E-180
score: 602.1
NoneNo IPR availableGENE3DG3DSA:2.60.40.2310coord: 631..726
e-value: 4.2E-26
score: 93.2
NoneNo IPR availablePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 7..726
NoneNo IPR availablePANTHERPTHR10795:SF350SUBTILISIN-LIKE PROTEASE SBT1.2coord: 7..726
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 351..477
e-value: 2.17062E-34
score: 127.145
IPR003137PA domainPFAMPF02225PAcoord: 391..463
e-value: 1.3E-8
score: 34.7
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 546..556
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 108..582
e-value: 7.60779E-135
score: 402.362