Carg16725 (gene) Silver-seed gourd

NameCarg16725
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionEnolase
LocationCucurbita_argyrosperma_scaffold_087 : 144443 .. 149677 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAACGATCGAGATCGTCAAAGCCCGTCAAATTTTTGATAGTCGTGGAAATCCTACTGTCGAAGTGAGTTTTAACTTCCTCTGAATCAATCCGTCGAAAATGTCTCACCGGAATTGTTACCTGTTATTCTACAATCGTCTTGTTGGTTTTTTAGCCTAACATTGGTTGCATTGTGTTTTTTTTTTTTTTTTTTTTTTCTGAGAAATGTGAGAATAGAAAGAGATTGTGAACCTATTGCGGTTATCGATTACTGTAACTGACCGTGTACATATAGATTCCTTGGCGTGAGTGTTGTACAGAGTTTGGGAAAATTTGAATGTGGAAGTTAGTGTGATCGCTATTGTAAATGATTGTTTGGTGTTTTTTTGTTTTGTTGATTTGAATTTGGTTTCAGGTTGACATAACTCTTACTGATGGAACGCTGGCCAGAGCTGCTGTTCCGAGCGGTGCTTCTACCGGTAAATCGATAGTGTTTGTTTATCTAACTGATTGAAAGTAATTATTCACGGTTTGACCTTTTCTGGATATAGATGACGAATGTATGGTAGGTGATGTTTCAATCGGTAGGTCGTTAGTGCTTGTTTATCGAAGAAAAGTAGATGCTACGAAATTTATTAAAAATGTACTTACCATACGTGAAAATTTTGAGAGGCGACCATTACATTTGTCTTGGAAACTTATTAAACATCTTCTGAGTGTGCTTTAAAAAAACTGAAATCACTGCGGCAAGCTTTAAGTATAGACATCTTTGAGGTCATGAGTTGTTCAACTTCAAACAAAACTGTAACCATATACCTTCAAGTTTCCCCTCAAAGAATTTTGCAAGGTCTAACAGTGATCCAAATGAGATTAGTAGAAAATTTGGTCAGAACATTCAATATGACTAATAAACAAAATGAAGGAAAACACAAATCCTTAAGACAGGGTATCATTTCATCTCTGCACGTATGTATTGTATATTTATCGTCACCTAATAGTTGTTAGATTTCATGATTTTCCCTTGTGCCCATTGATATAGTTGTATGAATGTCCTGTGCAAAAAAATGGTTTAAACATTTCGTTTATACTTCATAGTTTTAACTATCATATTTTATTGACTGAAGGTATCTATGAAGCTCTTGAGCTGAGAGATGGTGGGTCTGACTACCTTGGAAAGGGAGTACTTGAGGTTTGTATGGTAAAATCGGATATTTAAGTAAATTTCCCAGTGTTTGTTGTTAGGAACTTAGGAAATAACATTTTCCAAAGAAACATGTACTTTTTTTTTCTCTTGCATGTAAGACATTTTGCTATGATTGGAGGGAGAAAAACTATGCAGCAACTTTTTTCCTCTTGCATACAAGACTATTTGTGTCTGCGTGTTTTAAAGTTGCCTAATGTGACTGCATGACTTGATTTAGGCTGTGGAAAATGTAAATTCAATCATTGGACCTGCTTTAATTGGCAAGGTAAAAGCAATAATTTCATTAGCATTTTTTTTCTACTGTCAGTGAATATGAGTGAAGTTTATATAGTTCATTCAATTTTCTGTGGCATTTCCAAGGACCCAAGAGAGCAGACAAAACTAGACAATTTTATGGTGCGGCAACTTGATGGGACCGTCAATGAATGGGGTTGGTGCAAACAGAAGGCATGTTGAACCTTTTTTTGTTCCACCGAGGTTGATGTCTCCAATGTTATGCTAACTTAGGCTAACGGGATTTTCCCTCTTCATTCTCACAGCTTGGAGCAAATGCTATACTGGCAGTCTCCCTTGCTGTTTGTAAAGCTGGAGCTGCAGTGAACAAGATACCCCTCTATAAGGTATTTGTAGTCTCACGTGGTTTTAGAAGAGGGAATTCGTTTTGATTTTTTGTTTTTTTTTTCCTAGTCTTATATCGTCACTTGTCTTTCTTGTAACTTCTAGCACATTGCCAAGCTTGCTGGGAATAAGAAGTTGGTTCTTCCGGTCCCTGCATTCAACGTCATTAATGGAGGTTCTCATGCAGGCAATAAGCTAGCCATGCAGGTAATATGGTTTTGATATTTTTTCCCTTTTTGATAGATTAGGTGCTTTACTTTGGAATTTTGTGTTATTTTTCTGGTATGTAGGAATTTATGATTCTCCCTGTTGGAGCATCTTCTTTTAAGGAAGCTATGAAAATGGGTGTGGAAGTTTACCATCACCTCAAGGTTGTTTGCAATCCCCTTGTACCCCAGTTTTCTAAATTTATTTATACAAAGCGGTTGTTGTATCAATATATTTTGTCTGCTTTTCAGGCTGTAATAAAAAAGAAATATGGACAAGATGCTACTAATGTTGGTGATGAAGGCGGCTTTGCCCCTAATATTCAGGTCTTAGAAACATTAGCCTACAGCATATTGTTATTTTCAAAGTTGGAGTTTTCAATGATTATTTGGCTATTGATGTTTTTCTCTTCTTTCTTTAGTCCATTCCTCTTTTCCTTTATGGTTGGAGGGAAAGGTTTGTGATCGGACAAAAACGCAGATTCTAGTCAGATAGTTTCCTTCACCATTATTGTCATTTATGACTTATGATTGGTGTTTTTTATTTCACCACGCACACTACCGAGTGGCTAATATTGATTTATATAAATTTCAGGAGAACAAGGAGGGTCTTGAACTCTTGAAGGTTGCTATAGCGAAAGCTGGATACACTGGAAAGGTGCTCTCCTTTTGTCTTTTTATTAACGTATCGAATAATATATTTCCTGATAGTACCTAGGTCAAGTCCTTGATTATACTCTTTTGCCACTTCTAGGTTGTAATTGGAATGGATGTAGCTGCTTCAGAATTTTATGATAACAAGGCCAAGACCTATGACCTGAATTTTAAGGAAGAGGTATTGGACTGTTGGCTGGTCATGGTTCAAGTATTTTTCATGTTACACATCAGCCTGATGATAGATTTTGCTGCAGAACAATGATGGATCAGAAAAAATTTCTGGAGACAGCTTGAAGAATGTCTACAAGTCATTTGTGACAGATTATCCTATTGTGTCCATTGAAGATCCATTCGATCAGGATGATTGGGAGAATTATGCGAAGCTGACTAGTGAAATTGGCCGGCAAGTCCAGATAGTTGGAGACGACCTCTTGGTTACCAACCCAAAGGTCTTAAATGTTTTAATCATCATGTACATTCTGGTTTTCTGAATCAACATTCTGACATTATCTTGCATCTTGTAACAATTGCAGCGTGTAGAGAAAGCTATCAAGGAGAAGGCATGCAATTCCCTTCTCTTGAAGGTAATCACTCCACTTTTTCTTCCTCATAAGTATCCATTTTCCCTCTTATGTGCTTAACTTTCTTTTGGCAGCTATCTGTTTGTTACCATGCTTTAGTGCTCAAGTAATTTGCAGTATATTCATAATGTTGAGATTCTTATGATATATTTATCAGGTAAATCAAATTGGTTCAGTGACTGAAAGTATTGAAGCAGTCAAGTTGTCTAAGCACGCTGGTTGGGGTGTTATGGCAAGCCATAGAAGGTATGCTACGCTTCATTGCATAGCCATCTCCCCTTCTTTTTTTTTTCTTTTTTTTTTTCTTTAATTGCATATTATAATCTCTTTCACCCAGCCTCCATGTACTATTGATTATATTTTTCTCCTTTTTGTGCAATTTAGTGGGGAAACTGAGGATACGTTCATTGCAGACCTTTCAGTTGGCCTGTCAACAGTAAGTGCTAACAATTCCCCCATGAGTGAAACTCTGTAGTTTTACCTGTAAATTTGTTCGTAAAATTATTGTTACTGTATAAGCATGCCCCGCTCCCTGCATGGGCATATTGCTCATGCTATGAGTGATGATATTTCTGATGGAATATTAATCCAACATGTAGCCTTTTAAAGGAGAAAGTAACATTTATGTGCATTTTTTACTTGTTTGTTTTTGGAATTTGGTAGAGTTCAAAAGCATAATTTAAAAAAAAAAAACAAGTTTTTAAAATTTAATTCTTTTAGTTTTCAAAATTGGGCTTGGATAACCATTATCCAGTTGGTCATATTTAAATATCATTTCTTTGTTTGAGGTGGTACCAATCAAATGTACTAACGTTTACAAAGCATTGGTGGTAAATCTTTGTTTATGTGTGTGTATTTTGAGTGTTTTAAATTATCTTCAATTCCTTGACGGTCATTTGAATGTTTCCTAAACTGCAAAACAGGGTCAGATTAAGACTGGTGCACCCTGTAGATCTGAGCGCCTAGCCAAATATAACCAGGTGAATATTATTAGTTGAAATTTTTGCCTCTTGCAAATGAAGATTCGGATAGTGGGCAAGACCATCTAAATTTCTTTAAGGTTATTAGTTCTAGAAGTTGAAATTTAGTCTCCATATTTTCAGTAAATCTTAAAATTAGGGTAGCTTTATTTAATTATTGTCTGCTTACAATAACAAAACCTACAACTAAATAAGACTTTTTTTATGATTTGACCACCGGTTGACCTGTTTTTCAACTGTGATCTTTGCTTTTCCCAACATCCATACAGCTCAAATTTGGGCGCTTGTAACACAATCAACTGCTTTCTTTTTTTTTCCCTGCAGCTCCTTAGGATAGAAGAAGAGCTCGGACCAGCTGCAATTTATGCTGGATCAAAATTTCGATCACCAGTGGAGCCTTATTGAGATTTTAATCATGAGAATGCAACCACTTCACTATATTGATGATTTTGTATGGAACATATGAAATGAACTTTTTTCTGGTAGCAGGGAGGTGATGAGGTTCTCTACGCATGTTATATTAGACTAGATTTAATCTCGATTGTCTCTTTTACTAGAAATGCTGTGATTTTCTAGCCTGCTTCTTTCTGTTTCTATTGTTTTCTTTTTCGTTACTTTTCCTGGAAAAAATTACTTGGAGTGGGGGTTAGCAACCTGGTTCTAGCGAATTTAACCAAACTTTGGTCCCAGTCTGAGTTTTTACCCCCCAAAGATTGGACCTAAACAGGACTATGCACTCTTAACTCCTAGGTCCATCTATGGAAGATTGTCCTAGGAAGGGCCATTTTATTTGCAGGAACTTGCTGGTCGTTACCTTTAGATTTTAGATGCTCTAAAATTAAACAGCCAACATCTTTTAACTATGATATTTGCCAATCCAATAATTCTTTTATCATATTGCTACAGCTACTCCTTGACCTGATCAAATGAATTACTGCATTCATTAGGTAAAAATATTTTTTCTTTTGGTACAACGTGAGATCACTTTGGCATAGGTAAATTGATTT

mRNA sequence

ATGGCAACGATCGAGATCGTCAAAGCCCGTCAAATTTTTGATAGTCGTGGAAATCCTACTGTCGAAGTTGACATAACTCTTACTGATGGAACGCTGGCCAGAGCTGCTGTTCCGAGCGGTGCTTCTACCGGTATCTATGAAGCTCTTGAGCTGAGAGATGGTGGGTCTGACTACCTTGGAAAGGGAGTACTTGAGGCTGTGGAAAATGTAAATTCAATCATTGGACCTGCTTTAATTGGCAAGGACCCAAGAGAGCAGACAAAACTAGACAATTTTATGGTGCGGCAACTTGATGGGACCGTCAATGAATGGGGTTGGTGCAAACAGAAGCTTGGAGCAAATGCTATACTGGCAGTCTCCCTTGCTGTTTGTAAAGCTGGAGCTGCAGTGAACAAGATACCCCTCTATAAGCACATTGCCAAGCTTGCTGGGAATAAGAAGTTGGTTCTTCCGGTCCCTGCATTCAACGTCATTAATGGAGGTTCTCATGCAGGCAATAAGCTAGCCATGCAGGAATTTATGATTCTCCCTGTTGGAGCATCTTCTTTTAAGGAAGCTATGAAAATGGGTGTGGAAGTTTACCATCACCTCAAGGCTGTAATAAAAAAGAAATATGGACAAGATGCTACTAATGTTGGTGATGAAGGCGGCTTTGCCCCTAATATTCAGGAGAACAAGGAGGGTCTTGAACTCTTGAAGGTTGCTATAGCGAAAGCTGGATACACTGGAAAGGTTGTAATTGGAATGGATGTAGCTGCTTCAGAATTTTATGATAACAAGGCCAAGACCTATGACCTGAATTTTAAGGAAGAGAACAATGATGGATCAGAAAAAATTTCTGGAGACAGCTTGAAGAATGTCTACAAGTCATTTGTGACAGATTATCCTATTGTGTCCATTGAAGATCCATTCGATCAGGATGATTGGGAGAATTATGCGAAGCTGACTAGTGAAATTGGCCGGCAAGTCCAGATAGTTGGAGACGACCTCTTGGTTACCAACCCAAAGCGTGTAGAGAAAGCTATCAAGGAGAAGGCATGCAATTCCCTTCTCTTGAAGGTAAATCAAATTGGTTCAGTGACTGAAAGTATTGAAGCAGTCAAGTTGTCTAAGCACGCTGGTTGGGGTGTTATGGCAAGCCATAGAAGTGGGGAAACTGAGGATACGTTCATTGCAGACCTTTCAGTTGGCCTGTCAACAGGTCAGATTAAGACTGGTGCACCCTGTAGATCTGAGCGCCTAGCCAAATATAACCAGCTCCTTAGGATAGAAGAAGAGCTCGGACCAGCTGCAATTTATGCTGGATCAAAATTTCGATCACCAGTGGAGCCTTATTGAGATTTTAATCATGAGAATGCAACCACTTCACTATATTGATGATTTTGTATGGAACATATGAAATGAACTTTTTTCTGGTAGCAGGGAGGTGATGAGGTTCTCTACGCATGTTATATTAGACTAGATTTAATCTCGATTGTCTCTTTTACTAGAAATGCTGTGATTTTCTAGCCTGCTTCTTTCTGTTTCTATTGTTTTCTTTTTCGTTACTTTTCCTGGAAAAAATTACTTGGAGTGGGGGTTAGCAACCTGGTTCTAGCGAATTTAACCAAACTTTGGTCCCAGTCTGAGTTTTTACCCCCCAAAGATTGGACCTAAACAGGACTATGCACTCTTAACTCCTAGGTCCATCTATGGAAGATTGTCCTAGGAAGGGCCATTTTATTTGCAGGAACTTGCTGGTCGTTACCTTTAGATTTTAGATGCTCTAAAATTAAACAGCCAACATCTTTTAACTATGATATTTGCCAATCCAATAATTCTTTTATCATATTGCTACAGCTACTCCTTGACCTGATCAAATGAATTACTGCATTCATTAGGTAAAAATATTTTTTCTTTTGGTACAACGTGAGATCACTTTGGCATAGGTAAATTGATTT

Coding sequence (CDS)

ATGGCAACGATCGAGATCGTCAAAGCCCGTCAAATTTTTGATAGTCGTGGAAATCCTACTGTCGAAGTTGACATAACTCTTACTGATGGAACGCTGGCCAGAGCTGCTGTTCCGAGCGGTGCTTCTACCGGTATCTATGAAGCTCTTGAGCTGAGAGATGGTGGGTCTGACTACCTTGGAAAGGGAGTACTTGAGGCTGTGGAAAATGTAAATTCAATCATTGGACCTGCTTTAATTGGCAAGGACCCAAGAGAGCAGACAAAACTAGACAATTTTATGGTGCGGCAACTTGATGGGACCGTCAATGAATGGGGTTGGTGCAAACAGAAGCTTGGAGCAAATGCTATACTGGCAGTCTCCCTTGCTGTTTGTAAAGCTGGAGCTGCAGTGAACAAGATACCCCTCTATAAGCACATTGCCAAGCTTGCTGGGAATAAGAAGTTGGTTCTTCCGGTCCCTGCATTCAACGTCATTAATGGAGGTTCTCATGCAGGCAATAAGCTAGCCATGCAGGAATTTATGATTCTCCCTGTTGGAGCATCTTCTTTTAAGGAAGCTATGAAAATGGGTGTGGAAGTTTACCATCACCTCAAGGCTGTAATAAAAAAGAAATATGGACAAGATGCTACTAATGTTGGTGATGAAGGCGGCTTTGCCCCTAATATTCAGGAGAACAAGGAGGGTCTTGAACTCTTGAAGGTTGCTATAGCGAAAGCTGGATACACTGGAAAGGTTGTAATTGGAATGGATGTAGCTGCTTCAGAATTTTATGATAACAAGGCCAAGACCTATGACCTGAATTTTAAGGAAGAGAACAATGATGGATCAGAAAAAATTTCTGGAGACAGCTTGAAGAATGTCTACAAGTCATTTGTGACAGATTATCCTATTGTGTCCATTGAAGATCCATTCGATCAGGATGATTGGGAGAATTATGCGAAGCTGACTAGTGAAATTGGCCGGCAAGTCCAGATAGTTGGAGACGACCTCTTGGTTACCAACCCAAAGCGTGTAGAGAAAGCTATCAAGGAGAAGGCATGCAATTCCCTTCTCTTGAAGGTAAATCAAATTGGTTCAGTGACTGAAAGTATTGAAGCAGTCAAGTTGTCTAAGCACGCTGGTTGGGGTGTTATGGCAAGCCATAGAAGTGGGGAAACTGAGGATACGTTCATTGCAGACCTTTCAGTTGGCCTGTCAACAGGTCAGATTAAGACTGGTGCACCCTGTAGATCTGAGCGCCTAGCCAAATATAACCAGCTCCTTAGGATAGAAGAAGAGCTCGGACCAGCTGCAATTTATGCTGGATCAAAATTTCGATCACCAGTGGAGCCTTATTGA

Protein sequence

MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLGKGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVSLAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAGYTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSIEDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVTESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLLRIEEELGPAAIYAGSKFRSPVEPY
BLAST of Carg16725 vs. NCBI nr
Match: XP_022922793.1 (enolase-like [Cucurbita moschata])

HSP 1 Score: 875.9 bits (2262), Expect = 5.7e-251
Identity = 444/445 (99.78%), Postives = 444/445 (99.78%), Query Frame = 0

Query: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MA IEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 446
           LRIEEELGPAAIYAGSKFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Carg16725 vs. NCBI nr
Match: XP_023552596.1 (enolase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 875.5 bits (2261), Expect = 7.4e-251
Identity = 443/445 (99.55%), Postives = 444/445 (99.78%), Query Frame = 0

Query: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MA IEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MAAIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 446
           LRIEEELGPAAIYAGSKFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Carg16725 vs. NCBI nr
Match: XP_022984196.1 (enolase 2-like [Cucurbita maxima])

HSP 1 Score: 871.3 bits (2250), Expect = 1.4e-249
Identity = 442/445 (99.33%), Postives = 443/445 (99.55%), Query Frame = 0

Query: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGS YLG
Sbjct: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSAYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMV QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVWQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 446
           LRIEEELGPAAIYAGSKFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Carg16725 vs. NCBI nr
Match: XP_022141329.1 (enolase-like [Momordica charantia])

HSP 1 Score: 853.6 bits (2204), Expect = 3.0e-244
Identity = 427/445 (95.96%), Postives = 443/445 (99.55%), Query Frame = 0

Query: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MATI+IVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MATIKIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AV+NVNSIIGPALIGKDPREQT+LDNFMV++LDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLKAVDNVNSIIGPALIGKDPREQTELDNFMVQKLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK+AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKIAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYD+K KTYDLNFKEE+NDGSEKISGDSLKNVYKSFVTDYP+VSI
Sbjct: 241 YTGKVVIGMDVAASEFYDSKDKTYDLNFKEEHNDGSEKISGDSLKNVYKSFVTDYPVVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAKLTSEIG+QVQIVGDDLLVTNPKRV+KAIKEK CNSLLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKLTSEIGQQVQIVGDDLLVTNPKRVDKAIKEKTCNSLLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SKHAGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKHAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 446
           LRIEEELGPAAIYAGSKFRSPVEPY
Sbjct: 421 LRIEEELGPAAIYAGSKFRSPVEPY 445

BLAST of Carg16725 vs. NCBI nr
Match: OVA07690.1 (Enolase [Macleaya cordata])

HSP 1 Score: 828.6 bits (2139), Expect = 1.0e-236
Identity = 414/445 (93.03%), Postives = 433/445 (97.30%), Query Frame = 0

Query: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MATI+ VKARQIFDSRGNPTVEVD+TL+DGTLARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MATIKAVKARQIFDSRGNPTVEVDVTLSDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AVENVN IIGPALIGKDP +Q +LDNFMV+QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLKAVENVNGIIGPALIGKDPTKQVELDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGA+VNKIPLYKHIA LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGASVNKIPLYKHIANLAGNKTLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYD+K KTYDLNFKEENNDGS+KISGDSLKNVYKSFV DYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDDKDKTYDLNFKEENNDGSQKISGDSLKNVYKSFVADYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAK+TSEIG+QVQIVGDDLLVTNPKRVEKAIKEK CN+LLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKMTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SK AGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKQAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 446
           LRIEEELG AAIYAG+KFR+PVEPY
Sbjct: 421 LRIEEELGSAAIYAGAKFRTPVEPY 445

BLAST of Carg16725 vs. TAIR10
Match: AT2G36530.1 (Enolase)

HSP 1 Score: 764.6 bits (1973), Expect = 3.3e-221
Identity = 384/445 (86.29%), Postives = 412/445 (92.58%), Query Frame = 0

Query: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MATI +VKARQIFDSRGNPTVEVDI  ++G    AAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MATITVVKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGV +AV NVN+IIGPALIGKDP +QT +DNFMV +LDGT NEWGWCKQKLGANAILAVS
Sbjct: 61  KGVSKAVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVS 120

Query: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAVCKAGA V+ IPLYKHIA LAGN K+VLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAVCKAGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           +SFKEAMKMGVEVYHHLK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AI KAG
Sbjct: 181 ASFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFY ++ KTYDLNFKEENN+GS+KISGD+LK++YKSFV +YPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFY-SEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAK+T+E G +VQIVGDDLLVTNPKRV KAI EK+CN+LLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKMTTECGTEVQIVGDDLLVTNPKRVAKAIAEKSCNALLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SK AGWGVM SHRSGETEDTFIADL+VGLSTGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 446
           LRIEEELG  AIYAG  FR PVEPY
Sbjct: 421 LRIEEELGSEAIYAGVNFRKPVEPY 444

BLAST of Carg16725 vs. TAIR10
Match: AT1G74030.1 (enolase 1)

HSP 1 Score: 577.8 bits (1488), Expect = 5.8e-165
Identity = 302/436 (69.27%), Postives = 354/436 (81.19%), Query Frame = 0

Query: 7   VKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGG-SDYLGKGVLE 66
           VKARQI DSRGNPTVEVD+   D  L R+AVPSGASTGIYEALELRDG  S Y GKGVL+
Sbjct: 54  VKARQIIDSRGNPTVEVDLITDD--LYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLQ 113

Query: 67  AVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVSLAVCK 126
           A++N+N ++ P LIG D R Q  +D  M+ +LDGT N     K KLGANAIL VSL+VC+
Sbjct: 114 AIKNINELVAPKLIGVDVRNQADVDALML-ELDGTPN-----KSKLGANAILGVSLSVCR 173

Query: 127 AGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKE 186
           AGA    +PLYKHI + +G K+LV+PVPAFNVINGGSHAGN LAMQEFMILPVGA+SF E
Sbjct: 174 AGAGAKGVPLYKHIQETSGTKELVMPVPAFNVINGGSHAGNSLAMQEFMILPVGATSFSE 233

Query: 187 AMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAGYTGKV 246
           A +MG EVYH LK +IK KYGQDA NVGDEGGFAPN+Q+N+EGL LL  AI KAGYTGK+
Sbjct: 234 AFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 293

Query: 247 VIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSIEDPFD 306
            IGMDVAASEF+  K   YDLNFK++ NDG+  +S +SL ++Y+ F+ D+PIVSIEDPFD
Sbjct: 294 KIGMDVAASEFF-MKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFD 353

Query: 307 QDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVTESIE 366
           QDDW ++A L S +   +Q+VGDDLLVTNPKR+ +AIK+++CN+LLLKVNQIG+VTESI+
Sbjct: 354 QDDWSSWASLQSSV--DIQLVGDDLLVTNPKRIAEAIKKQSCNALLLKVNQIGTVTESIQ 413

Query: 367 AVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLLRIEE 426
           A   SK AGWGVM SHRSGETED FIADLSVGL++GQIKTGAPCRSERL+KYNQLLRIEE
Sbjct: 414 AALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLSKYNQLLRIEE 473

Query: 427 ELGPAAIYAGSKFRSP 442
           ELG    YAG  FRSP
Sbjct: 474 ELGNVR-YAGEAFRSP 477

BLAST of Carg16725 vs. TAIR10
Match: AT2G29560.1 (cytosolic enolase)

HSP 1 Score: 468.4 bits (1204), Expect = 5.0e-132
Identity = 250/439 (56.95%), Postives = 315/439 (71.75%), Query Frame = 0

Query: 2   ATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSD-YLG 61
           + I  VKARQI DSRG PTVEVD+    G   RA+VPSG S+G YEA+ELRDG    YLG
Sbjct: 45  SVITKVKARQILDSRGIPTVEVDLHTNKGVF-RASVPSGDSSGTYEAIELRDGDKGMYLG 104

Query: 62  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 121
             V +AV+N+N  I  ALIG DP+ Q ++D  M+  LD T       K +LGANAILAVS
Sbjct: 105 NSVAKAVKNINEKISEALIGMDPKLQGQIDQAMI-DLDKTEK-----KSELGANAILAVS 164

Query: 122 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 181
           +A CKAGAA  ++PL KH++ L+G   +VLPVPAF V++GG HA N  A+QE MILP+GA
Sbjct: 165 IAACKAGAAEKEVPLCKHLSDLSGRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGA 224

Query: 182 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 241
           S F+EA++ G E YHHLKAVI +K G    NVG++GG AP+I   KEGLEL+K AI + G
Sbjct: 225 SRFEEALQWGSETYHHLKAVISEKNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTG 284

Query: 242 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 301
           Y  K+ I +D+AA+ F       YDL+ K  N  G    S + + ++YK    DYPIVSI
Sbjct: 285 YNDKIKIAIDIAATNFC--LGTKYDLDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSI 344

Query: 302 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 361
           EDPFD++DWE + K  S +G   Q+VGDDLL++N KRVE+AI+E +CN+LLLKVNQIG+V
Sbjct: 345 EDPFDKEDWE-HTKYFSSLG-ICQVVGDDLLMSNSKRVERAIQESSCNALLLKVNQIGTV 404

Query: 362 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 421
           TE+IE VK+++ A WGV+ SHR GETED+FI+DLSVGL+TG IK GAPCR ER  KYNQL
Sbjct: 405 TEAIEVVKMARDAQWGVVTSHRCGETEDSFISDLSVGLATGVIKAGAPCRGERTMKYNQL 464

Query: 422 LRIEEELGPAAIYAGSKFR 440
           LRIEEELG  A+YAG  ++
Sbjct: 465 LRIEEELGDQAVYAGEDWK 472

BLAST of Carg16725 vs. Swiss-Prot
Match: sp|Q42971|ENO_ORYSJ (Enolase OS=Oryza sativa subsp. japonica OX=39947 GN=ENO1 PE=1 SV=2)

HSP 1 Score: 785.4 bits (2027), Expect = 3.3e-226
Identity = 388/444 (87.39%), Postives = 420/444 (94.59%), Query Frame = 0

Query: 2   ATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLGK 61
           ATI  VKARQIFDSRGNPTVEVD+  +DGT ARAAVPSGASTG+YEALELRDGGSDYLGK
Sbjct: 3   ATIVSVKARQIFDSRGNPTVEVDVCCSDGTFARAAVPSGASTGVYEALELRDGGSDYLGK 62

Query: 62  GVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVSL 121
           GV +AV+NVNS+I PALIGKDP  Q +LDNFMV+QLDGT NEWGWCKQKLGANAILAVSL
Sbjct: 63  GVSKAVDNVNSVIAPALIGKDPTSQAELDNFMVQQLDGTKNEWGWCKQKLGANAILAVSL 122

Query: 122 AVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAS 181
           A+CKAGA + KIPLY+HIA LAGNK+LVLPVPAFNVINGGSHAGNKLAMQEFMILP GA+
Sbjct: 123 AICKAGAIIKKIPLYQHIANLAGNKQLVLPVPAFNVINGGSHAGNKLAMQEFMILPTGAA 182

Query: 182 SFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAGY 241
           SFKEAMKMGVEVYH+LK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AI KAGY
Sbjct: 183 SFKEAMKMGVEVYHNLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGY 242

Query: 242 TGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSIE 301
           TGKVVIGMDVAASEFY++K KTYDLNFKEENNDGS+KISGDSLKNVYKSFV++YPIVSIE
Sbjct: 243 TGKVVIGMDVAASEFYNDKDKTYDLNFKEENNDGSQKISGDSLKNVYKSFVSEYPIVSIE 302

Query: 302 DPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVT 361
           DPFDQDDWE+YAK+T+EIG QVQIVGDDLLVTNP RV KAI+EK+CN+LLLKVNQIGSVT
Sbjct: 303 DPFDQDDWEHYAKMTAEIGEQVQIVGDDLLVTNPTRVAKAIQEKSCNALLLKVNQIGSVT 362

Query: 362 ESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLL 421
           ESIEAVK+SK AGWGVM SHRSGETEDTFIADL+VGL+TGQIKTGAPCRSERLAKYNQLL
Sbjct: 363 ESIEAVKMSKRAGWGVMTSHRSGETEDTFIADLAVGLATGQIKTGAPCRSERLAKYNQLL 422

Query: 422 RIEEELGPAAIYAGSKFRSPVEPY 446
           RIEEELG AA+YAG+KFR+PVEPY
Sbjct: 423 RIEEELGAAAVYAGAKFRAPVEPY 446

BLAST of Carg16725 vs. Swiss-Prot
Match: sp|P42896|ENO_RICCO (Enolase OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 7.4e-226
Identity = 394/443 (88.94%), Postives = 420/443 (94.81%), Query Frame = 0

Query: 3   TIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLGKG 62
           TI  V+ARQIFDSRGNPTVE DI L+DG LARAAVPSGASTGIYEALELRDGGSDYLGKG
Sbjct: 4   TIVSVRARQIFDSRGNPTVEADIKLSDGHLARAAVPSGASTGIYEALELRDGGSDYLGKG 63

Query: 63  VLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVSLA 122
           V +AVENVNSIIGPALIGKDP EQT LDNFMV++LDGTVNEWGWCKQKLGANAILAVSLA
Sbjct: 64  VSKAVENVNSIIGPALIGKDPTEQTALDNFMVQELDGTVNEWGWCKQKLGANAILAVSLA 123

Query: 123 VCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 182
           +CKAGA V  IPLYKHIA LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS
Sbjct: 124 LCKAGAHVKGIPLYKHIANLAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 183

Query: 183 FKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAGYT 242
           FKEAMKMG EVYHHLK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAGYT
Sbjct: 184 FKEAMKMGAEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAGYT 243

Query: 243 GKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSIED 302
           GKVVIGMDVAASEFY +  KTYDLNFKEENNDGS+KISG++LK++YKSF ++YPIVSIED
Sbjct: 244 GKVVIGMDVAASEFYGSD-KTYDLNFKEENNDGSQKISGEALKDLYKSFASEYPIVSIED 303

Query: 303 PFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVTE 362
           PFDQDDWE+Y+KLTSEIG +VQIVGDDLLVTNPKRVEKAI+EKACN+LLLKVNQIGSVTE
Sbjct: 304 PFDQDDWEHYSKLTSEIGEKVQIVGDDLLVTNPKRVEKAIQEKACNALLLKVNQIGSVTE 363

Query: 363 SIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLLR 422
           SIEAV++SK AGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQLLR
Sbjct: 364 SIEAVRMSKRAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLR 423

Query: 423 IEEELGPAAIYAGSKFRSPVEPY 446
           IEEELG  A+YAG+KFR+PVEPY
Sbjct: 424 IEEELGAEAVYAGAKFRTPVEPY 445

BLAST of Carg16725 vs. Swiss-Prot
Match: sp|P42895|ENO2_MAIZE (Enolase 2 OS=Zea mays OX=4577 GN=ENO2 PE=2 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 9.6e-226
Identity = 388/444 (87.39%), Postives = 418/444 (94.14%), Query Frame = 0

Query: 2   ATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLGK 61
           ATI+ VKARQIFDSRGNPTVEVD+  +DGT ARAAVPSGASTG+YEALELRDGGS YLGK
Sbjct: 3   ATIQSVKARQIFDSRGNPTVEVDVFCSDGTFARAAVPSGASTGVYEALELRDGGSYYLGK 62

Query: 62  GVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVSL 121
           GV +AV NVNS+IGPALIGKDP  QT++DNFMV+QLDGT NEWGWCKQKLGANAILAVSL
Sbjct: 63  GVSKAVNNVNSVIGPALIGKDPTAQTEIDNFMVQQLDGTKNEWGWCKQKLGANAILAVSL 122

Query: 122 AVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAS 181
           AVCKAGA++ +IPLY+HIA LAGNK+LVLPVPAFNVINGGSHAGNKLAMQEFMILP GA+
Sbjct: 123 AVCKAGASIKRIPLYQHIANLAGNKQLVLPVPAFNVINGGSHAGNKLAMQEFMILPTGAA 182

Query: 182 SFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAGY 241
           SFKEAMKMGVEVYHHLK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AI KAGY
Sbjct: 183 SFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGY 242

Query: 242 TGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSIE 301
           TGKVVIGMDVAASEFY +K +TYDLNFKEENNDGS+KISGDSLKNVYKSFV++YPIVSIE
Sbjct: 243 TGKVVIGMDVAASEFYSDKDQTYDLNFKEENNDGSQKISGDSLKNVYKSFVSEYPIVSIE 302

Query: 302 DPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVT 361
           DPFDQDDW +YAK+T EIG QVQIVGDDLLVTNP RV KAIKEK+CN+LLLKVNQIGSVT
Sbjct: 303 DPFDQDDWVHYAKMTEEIGEQVQIVGDDLLVTNPTRVAKAIKEKSCNALLLKVNQIGSVT 362

Query: 362 ESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLL 421
           ESIEAVK+SK AGWGVM SHRSGETEDTFIADL+VGLSTGQIKTGAPCRSERLAKYNQLL
Sbjct: 363 ESIEAVKMSKRAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLL 422

Query: 422 RIEEELGPAAIYAGSKFRSPVEPY 446
           RIEEELG  A+YAG+KFR+PVEPY
Sbjct: 423 RIEEELGAIAVYAGAKFRAPVEPY 446

BLAST of Carg16725 vs. Swiss-Prot
Match: sp|Q9LEI9|ENO2_HEVBR (Enolase 2 OS=Hevea brasiliensis OX=3981 GN=ENO2 PE=1 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 2.4e-224
Identity = 392/443 (88.49%), Postives = 416/443 (93.91%), Query Frame = 0

Query: 3   TIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLGKG 62
           TI  V+ARQIFDSRGNPTVE D+ L+DG LARAAVP GASTGIYEALELRDGGSDYLGKG
Sbjct: 4   TIVSVRARQIFDSRGNPTVEADVKLSDGYLARAAVPRGASTGIYEALELRDGGSDYLGKG 63

Query: 63  VLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVSLA 122
           V +AVENVN IIGPAL+GKDP +Q  +DNFMV+QLDGTVNEWGWCKQKLGANAILAVSLA
Sbjct: 64  VSKAVENVNIIIGPALVGKDPTDQVGIDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLA 123

Query: 123 VCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 182
           VCKAGA V  IPLYKH+A LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS
Sbjct: 124 VCKAGAHVKGIPLYKHVANLAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 183

Query: 183 FKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAGYT 242
           FKEAMKMG EVYHHLK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAGYT
Sbjct: 184 FKEAMKMGAEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAGYT 243

Query: 243 GKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSIED 302
           GKVVIGMDVAASEFY +  KTYDLNFKEENN+GS+KISGD LK++YKSFVT+YPIVSIED
Sbjct: 244 GKVVIGMDVAASEFYGSD-KTYDLNFKEENNNGSQKISGDVLKDLYKSFVTEYPIVSIED 303

Query: 303 PFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVTE 362
           PFDQDDWE+YAKLTSEIG +VQIVGDDLLVTNPKRVEKAIKEKACN+LLLKVNQIGSVTE
Sbjct: 304 PFDQDDWEHYAKLTSEIGVKVQIVGDDLLVTNPKRVEKAIKEKACNALLLKVNQIGSVTE 363

Query: 363 SIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLLR 422
           SIEAVK+SK AGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQLLR
Sbjct: 364 SIEAVKMSKRAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLR 423

Query: 423 IEEELGPAAIYAGSKFRSPVEPY 446
           IEEELG  A+YAG+ FR+PVEPY
Sbjct: 424 IEEELGAEAVYAGANFRTPVEPY 445

BLAST of Carg16725 vs. Swiss-Prot
Match: sp|P26300|ENO_SOLLC (Enolase OS=Solanum lycopersicum OX=4081 GN=PGH1 PE=2 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 1.2e-223
Identity = 388/445 (87.19%), Postives = 414/445 (93.03%), Query Frame = 0

Query: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MATI+ +KARQIFDSRGNPTVEVD+ +++G  ARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MATIKSIKARQIFDSRGNPTVEVDVHISNGVFARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGV +AV NVNSIIGPAL+GKDP +QT LDNFMV QLDGT NEWGWCK+KLGANAILAVS
Sbjct: 61  KGVSKAVNNVNSIIGPALVGKDPTDQTGLDNFMVHQLDGTQNEWGWCKEKLGANAILAVS 120

Query: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAVCKAGAAV  +PLYKHIA LAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAVCKAGAAVRNVPLYKHIADLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           ++FKEAMKMG EVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AI KAG
Sbjct: 181 ANFKEAMKMGCEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFY  K K+YDLNFKEE+NDGS+KISGD LK++YKSFV++YPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFY-GKDKSYDLNFKEESNDGSQKISGDQLKDLYKSFVSEYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE YAKLT+EIG QVQIVGDDLLVTNPKRV KAI EK CN+LLLKVNQIGSV
Sbjct: 301 EDPFDQDDWETYAKLTAEIGEQVQIVGDDLLVTNPKRVAKAIAEKTCNALLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SK AGWGVM SHRSGETEDTFIADL+VGLSTGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 446
           LRIEEELG  A+YAG+ FR PVEPY
Sbjct: 421 LRIEEELGSEAVYAGASFRKPVEPY 444

BLAST of Carg16725 vs. TrEMBL
Match: tr|A0A200QB48|A0A200QB48_9MAGN (Enolase OS=Macleaya cordata OX=56857 GN=BVC80_1827g52 PE=3 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 6.9e-237
Identity = 414/445 (93.03%), Postives = 433/445 (97.30%), Query Frame = 0

Query: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MATI+ VKARQIFDSRGNPTVEVD+TL+DGTLARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MATIKAVKARQIFDSRGNPTVEVDVTLSDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AVENVN IIGPALIGKDP +Q +LDNFMV+QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLKAVENVNGIIGPALIGKDPTKQVELDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LA+CKAGA+VNKIPLYKHIA LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAICKAGASVNKIPLYKHIANLAGNKTLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYD+K KTYDLNFKEENNDGS+KISGDSLKNVYKSFV DYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDDKDKTYDLNFKEENNDGSQKISGDSLKNVYKSFVADYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAK+TSEIG+QVQIVGDDLLVTNPKRVEKAIKEK CN+LLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKMTSEIGQQVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SK AGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKQAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 446
           LRIEEELG AAIYAG+KFR+PVEPY
Sbjct: 421 LRIEEELGSAAIYAGAKFRTPVEPY 445

BLAST of Carg16725 vs. TrEMBL
Match: tr|A0A1R3JT80|A0A1R3JT80_9ROSI (Enolase OS=Corchorus olitorius OX=93759 GN=COLO4_14178 PE=3 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 9.0e-237
Identity = 415/445 (93.26%), Postives = 430/445 (96.63%), Query Frame = 0

Query: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MATI+ VKARQIFDSRGNPTVEVD+TL+DGTLARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MATIKCVKARQIFDSRGNPTVEVDVTLSDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AVENVN  IGPAL+GKDP EQ K+DNFMV+QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLKAVENVNGTIGPALVGKDPTEQAKIDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAVCKAGA V KIPLY+HIA LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAVCKAGAMVKKIPLYQHIANLAGNKTLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYDNK KTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKDKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAKLTSEIG QVQIVGDDLLVTNPKRVEKAIKEK CN+LLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKLTSEIGEQVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SK AGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKCAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 446
           LRIEEELG AA+YAG+KFR+PVEPY
Sbjct: 421 LRIEEELGAAAVYAGAKFRAPVEPY 445

BLAST of Carg16725 vs. TrEMBL
Match: tr|D7T227|D7T227_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_16s0022g01770 PE=3 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 1.2e-236
Identity = 411/445 (92.36%), Postives = 433/445 (97.30%), Query Frame = 0

Query: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MATI+++KARQIFDSRGNPTVEVD+TL+DGT ARAAVPSGASTGIYEALELRDGGSD+LG
Sbjct: 1   MATIKVIKARQIFDSRGNPTVEVDVTLSDGTFARAAVPSGASTGIYEALELRDGGSDFLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AVENVN+II PALIGKDP EQ K+DNFMV+QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLKAVENVNAIIAPALIGKDPTEQVKIDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAVCKAGA VNKIPLYKHIA LAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILP+GA
Sbjct: 121 LAVCKAGAMVNKIPLYKHIANLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPLGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLK+VIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYDNK KTYDLNFKEENNDGS+KISGDSLKNVYKSFV DYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKDKTYDLNFKEENNDGSQKISGDSLKNVYKSFVNDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAK+TSEIG +VQIVGDDLLVTNPKRVEKAIKEKACN+LLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKMTSEIGEKVQIVGDDLLVTNPKRVEKAIKEKACNALLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SK AGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKRAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 446
           LRIEEELG AA+YAG+KFR+PVEPY
Sbjct: 421 LRIEEELGSAAVYAGAKFRAPVEPY 445

BLAST of Carg16725 vs. TrEMBL
Match: tr|M5XJJ3|M5XJJ3_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_2G281900 PE=3 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 2.0e-236
Identity = 414/443 (93.45%), Postives = 430/443 (97.07%), Query Frame = 0

Query: 3   TIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLGKG 62
           TI++VKARQIFDSRGNPTVEVD+TL DGT ARAAVPSGASTGIYEALELRDGGSDYLGKG
Sbjct: 4   TIKLVKARQIFDSRGNPTVEVDVTLFDGTYARAAVPSGASTGIYEALELRDGGSDYLGKG 63

Query: 63  VLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVSLA 122
           VL+AVENVNSIIGPALIGKDP EQTK+DN+MV+QLDGTVNEWGWCKQKLGANAILAVSLA
Sbjct: 64  VLKAVENVNSIIGPALIGKDPTEQTKIDNYMVQQLDGTVNEWGWCKQKLGANAILAVSLA 123

Query: 123 VCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 182
           VCKAGA VNKIPLYKHIA LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS
Sbjct: 124 VCKAGAIVNKIPLYKHIANLAGNKTLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 183

Query: 183 FKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAGYT 242
           FKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAGYT
Sbjct: 184 FKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAGYT 243

Query: 243 GKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSIED 302
           GKVVIGMDVAASEFYDNK KTYDLNFKEE NDGS+KISGDSLKNVYKSFVT+YPIVSIED
Sbjct: 244 GKVVIGMDVAASEFYDNKDKTYDLNFKEEKNDGSQKISGDSLKNVYKSFVTEYPIVSIED 303

Query: 303 PFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSVTE 362
           PFDQDDWE+YAK+T+EIG QVQIVGDDLLVTNPKRVEKAIKEK+CN+LLLKVNQIGSVTE
Sbjct: 304 PFDQDDWEHYAKMTAEIGEQVQIVGDDLLVTNPKRVEKAIKEKSCNALLLKVNQIGSVTE 363

Query: 363 SIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLLR 422
           SIEAVK+SK AGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLLR
Sbjct: 364 SIEAVKMSKRAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQLLR 423

Query: 423 IEEELGPAAIYAGSKFRSPVEPY 446
           IEEELG AA+YAGSKFR PVEPY
Sbjct: 424 IEEELGSAAVYAGSKFRVPVEPY 446

BLAST of Carg16725 vs. TrEMBL
Match: tr|A0A1U8P078|A0A1U8P078_GOSHI (enolase OS=Gossypium hirsutum OX=3635 GN=LOC107953663 PE=3 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 5.8e-236
Identity = 411/445 (92.36%), Postives = 432/445 (97.08%), Query Frame = 0

Query: 1   MATIEIVKARQIFDSRGNPTVEVDITLTDGTLARAAVPSGASTGIYEALELRDGGSDYLG 60
           MATI+ VKARQIFDSRGNPTVEVD++L+DGT+ARAAVPSGASTGIYEALELRDGGSDYLG
Sbjct: 1   MATIKCVKARQIFDSRGNPTVEVDVSLSDGTVARAAVPSGASTGIYEALELRDGGSDYLG 60

Query: 61  KGVLEAVENVNSIIGPALIGKDPREQTKLDNFMVRQLDGTVNEWGWCKQKLGANAILAVS 120
           KGVL+AVENVN+IIGPAL+GKDP EQ K+DNFMV+QLDGTVNEWGWCKQKLGANAILAVS
Sbjct: 61  KGVLKAVENVNTIIGPALVGKDPTEQAKIDNFMVQQLDGTVNEWGWCKQKLGANAILAVS 120

Query: 121 LAVCKAGAAVNKIPLYKHIAKLAGNKKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
           LAVCKAGA + KIPLY+HIA LAGNK LVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA
Sbjct: 121 LAVCKAGAMLKKIPLYQHIANLAGNKTLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180

Query: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKVAIAKAG 240
           SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLK AIAKAG
Sbjct: 181 SSFKEAMKMGVEVYHHLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAG 240

Query: 241 YTGKVVIGMDVAASEFYDNKAKTYDLNFKEENNDGSEKISGDSLKNVYKSFVTDYPIVSI 300
           YTGKVVIGMDVAASEFYDNK KTYDLNFKEENNDGS+KISGDSLKNVYKSFVTDYPIVSI
Sbjct: 241 YTGKVVIGMDVAASEFYDNKDKTYDLNFKEENNDGSQKISGDSLKNVYKSFVTDYPIVSI 300

Query: 301 EDPFDQDDWENYAKLTSEIGRQVQIVGDDLLVTNPKRVEKAIKEKACNSLLLKVNQIGSV 360
           EDPFDQDDWE+YAK+TSEIG QVQIVGDDLLVTNPKRVEKAIKEK CN+LLLKVNQIGSV
Sbjct: 301 EDPFDQDDWEHYAKMTSEIGEQVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKVNQIGSV 360

Query: 361 TESIEAVKLSKHAGWGVMASHRSGETEDTFIADLSVGLSTGQIKTGAPCRSERLAKYNQL 420
           TESIEAVK+SK AGWGVMASHRSGETEDTFIADLSVGL+TGQIKTGAPCRSERLAKYNQL
Sbjct: 361 TESIEAVKMSKSAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQL 420

Query: 421 LRIEEELGPAAIYAGSKFRSPVEPY 446
           LRIEEELG AA+YAG+KFR+PVEPY
Sbjct: 421 LRIEEELGAAAVYAGAKFRAPVEPY 445

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022922793.15.7e-25199.78enolase-like [Cucurbita moschata][more]
XP_023552596.17.4e-25199.55enolase-like [Cucurbita pepo subsp. pepo][more]
XP_022984196.11.4e-24999.33enolase 2-like [Cucurbita maxima][more]
XP_022141329.13.0e-24495.96enolase-like [Momordica charantia][more]
OVA07690.11.0e-23693.03Enolase [Macleaya cordata][more]
Match NameE-valueIdentityDescription
AT2G36530.13.3e-22186.29Enolase[more]
AT1G74030.15.8e-16569.27enolase 1[more]
AT2G29560.15.0e-13256.95cytosolic enolase[more]
Match NameE-valueIdentityDescription
sp|Q42971|ENO_ORYSJ3.3e-22687.39Enolase OS=Oryza sativa subsp. japonica OX=39947 GN=ENO1 PE=1 SV=2[more]
sp|P42896|ENO_RICCO7.4e-22688.94Enolase OS=Ricinus communis OX=3988 PE=2 SV=1[more]
sp|P42895|ENO2_MAIZE9.6e-22687.39Enolase 2 OS=Zea mays OX=4577 GN=ENO2 PE=2 SV=1[more]
sp|Q9LEI9|ENO2_HEVBR2.4e-22488.49Enolase 2 OS=Hevea brasiliensis OX=3981 GN=ENO2 PE=1 SV=1[more]
sp|P26300|ENO_SOLLC1.2e-22387.19Enolase OS=Solanum lycopersicum OX=4081 GN=PGH1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A200QB48|A0A200QB48_9MAGN6.9e-23793.03Enolase OS=Macleaya cordata OX=56857 GN=BVC80_1827g52 PE=3 SV=1[more]
tr|A0A1R3JT80|A0A1R3JT80_9ROSI9.0e-23793.26Enolase OS=Corchorus olitorius OX=93759 GN=COLO4_14178 PE=3 SV=1[more]
tr|D7T227|D7T227_VITVI1.2e-23692.36Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_16s0022g01770 PE=3 SV=... [more]
tr|M5XJJ3|M5XJJ3_PRUPE2.0e-23693.45Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_2G281900 PE=3 SV=1[more]
tr|A0A1U8P078|A0A1U8P078_GOSHI5.8e-23692.36enolase OS=Gossypium hirsutum OX=3635 GN=LOC107953663 PE=3 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0046872metal ion binding
GO:0000287magnesium ion binding
GO:0004634phosphopyruvate hydratase activity
Vocabulary: Cellular Component
TermDefinition
GO:0000015phosphopyruvate hydratase complex
Vocabulary: Biological Process
TermDefinition
GO:0006096glycolytic process
Vocabulary: INTERPRO
TermDefinition
IPR020809Enolase_CS
IPR034390Enolase-like_superfamily
IPR036849Enolase-like_C_sf
IPR029017Enolase-like_N
IPR020810Enolase_C
IPR020811Enolase_N
IPR000941Enolase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006096 glycolytic process
biological_process GO:0009094 L-phenylalanine biosynthetic process
biological_process GO:0000162 tryptophan biosynthetic process
biological_process GO:0006571 tyrosine biosynthetic process
cellular_component GO:0000015 phosphopyruvate hydratase complex
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004634 phosphopyruvate hydratase activity
molecular_function GO:0046872 metal ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16725-RACarg16725-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000941EnolasePRINTSPR00148ENOLASEcoord: 379..396
score: 83.84
coord: 327..338
score: 75.51
coord: 350..364
score: 80.2
coord: 169..182
score: 77.06
coord: 37..51
score: 88.38
coord: 112..128
score: 82.98
IPR000941EnolasePIRSFPIRSF001400Enolasecoord: 1..440
e-value: 1.8E-190
score: 631.0
IPR000941EnolaseTIGRFAMTIGR01060TIGR01060coord: 4..439
e-value: 2.6E-174
score: 577.8
IPR000941EnolaseSFLDSFLDG00178enolasecoord: 2..427
e-value: 0.0
score: 642.1
IPR000941EnolasePANTHERPTHR11902ENOLASEcoord: 1..443
IPR000941EnolaseHAMAPMF_00318Enolasecoord: 2..427
score: 44.408
IPR000941EnolaseCDDcd03313enolasecoord: 7..426
e-value: 0.0
score: 753.933
IPR020811Enolase, N-terminalSMARTSM01193Enolase_N_3coord: 4..139
e-value: 3.8E-82
score: 288.9
IPR020811Enolase, N-terminalPFAMPF03952Enolase_Ncoord: 4..139
e-value: 2.2E-53
score: 179.8
IPR020810Enolase, C-terminal TIM barrel domainSMARTSM01192Enolase_C_3coord: 147..441
e-value: 7.7E-204
score: 693.1
IPR020810Enolase, C-terminal TIM barrel domainPFAMPF00113Enolase_Ccoord: 148..440
e-value: 7.0E-158
score: 524.4
IPR029017Enolase-like, N-terminalGENE3DG3DSA:3.30.390.10coord: 1..131
e-value: 2.3E-57
score: 194.9
IPR036849Enolase-like, C-terminal domain superfamilyGENE3DG3DSA:3.20.20.120coord: 133..445
e-value: 1.1E-155
score: 519.4
IPR036849Enolase-like, C-terminal domain superfamilySUPERFAMILYSSF51604Enolase C-terminal domain-likecoord: 147..440
IPR034390Enolase-like superfamilySFLDSFLDS00001Enolasecoord: 4..428
e-value: 0.0
score: 284.6
NoneNo IPR availableSFLDSFLDF00002enolasecoord: 2..427
e-value: 0.0
score: 642.1
NoneNo IPR availablePANTHERPTHR11902:SF32SUBFAMILY NOT NAMEDcoord: 1..443
NoneNo IPR availableSUPERFAMILYSSF54826Enolase N-terminal domain-likecoord: 4..143
IPR020809Enolase, conserved sitePROSITEPS00164ENOLASEcoord: 350..363