Carg16490 (gene) Silver-seed gourd

BLAST of Carg16490 vs. NCBI nr
Match: XP_022932543.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata])

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 570/572 (99.65%), Postives = 570/572 (99.65%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAVALNDD STYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

BLAST of Carg16490 vs. NCBI nr
Match: XP_023531470.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 563/572 (98.43%), Postives = 563/572 (98.43%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAVALNDD STYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAVSNLKASKASKQEIG       KAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

BLAST of Carg16490 vs. NCBI nr
Match: XP_022965912.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita maxima])

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 559/572 (97.73%), Postives = 560/572 (97.90%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPPIDQLSAV LNDD STYKSLKAEFSDRVPIRTIVCRPDGGSGLAG KVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVTLNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKIELRAEAVVHVG VDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGLVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAVSNLKASKASKQEIG       +AKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVLELKQAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

BLAST of Carg16490 vs. NCBI nr
Match: XP_022961000.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata])

HSP 1 Score: 1060.4 bits (2741), Expect = 2.1e-306
Identity = 529/572 (92.48%), Postives = 548/572 (95.80%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPP DQL+AV LNDD  TY SLKAEFSDRVPIR+IV RPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA++ADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRA+AVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAV+ LKA         XXXXXXX   ENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKAXXXXXXXXXXXXXXXXXXXENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAE+YVRFLCQWLLDNCLDDM+FMADKYDRTCIDRLKLVSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+ LPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

BLAST of Carg16490 vs. NCBI nr
Match: XP_023520843.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1059.7 bits (2739), Expect = 3.6e-306
Identity = 529/572 (92.48%), Postives = 547/572 (95.63%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEH APP DQL+AV LNDD  TY SLKAEFSDRVPIR+IV RPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHPAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA++ADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRA+AVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAV+ LKA         XXXXXXX   ENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKAXXXXXXXXXXXXXXXXXXXENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDM+FMADKYDRTCIDRLKLVSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+ LPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

BLAST of Carg16490 vs. TAIR10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein)

HSP 1 Score: 847.4 bits (2188), Expect = 5.0e-246
Identity = 409/574 (71.25%), Postives = 487/574 (84.84%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MA+   PP  QL+AV+L +D ST +  +A+FS+RV IRTI+ RPDGG+GLAG  VR+GGW
Sbjct: 1   MADEIVPPATQLAAVSLENDGSTVQ--RAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VK+GR   K  F+FL +NDGSCP NLQV+++ ++ D+  +V TGTCV VDGV K+PP  K
Sbjct: 61  VKSGRDQGKRTFSFLAVNDGSCPANLQVMVDPSLYDVSNLVATGTCVTVDGVLKVPPKGK 120

Query: 121 --HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q+IEL    V+ VG VD ++YPLPKTKLTLE LRD +HLR RTN+ISAVARIRNALA
Sbjct: 121 GTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQ TTL++  ERLE++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IV E+G  V+ LKA+KASK+ I         AKE  A ++ER++L+PG+P+KDG +
Sbjct: 241 AARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNI 300

Query: 301 DYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY +DFF RQAFLTVSGQLQVETYACALS+VYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FADL+DDMNCAEAYV+++C WLL+ C  DM+ MA  +D  CIDRLKLV+STPF R +YT+
Sbjct: 361 FADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTK 420

Query: 421 AIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 480
           AIE+LEEA+ KGKEF+N VEWGIDLASEHERYLTEV+F+KP+IVYNYPKGIKAFYMRLND
Sbjct: 421 AIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLND 480

Query: 481 DSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHS 540
           D KTVAAMDVLVPKVGELIGGSQREER++VI+ RI+E+GLP+EPYEWYLDLRRYGTVKH 
Sbjct: 481 DEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHC 540

Query: 541 GFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GFGLGFERM+LFATG+DNIRDVIPFPRYPG+ADL
Sbjct: 541 GFGLGFERMILFATGLDNIRDVIPFPRYPGKADL 572

BLAST of Carg16490 vs. TAIR10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein)

HSP 1 Score: 795.0 bits (2052), Expect = 3.0e-230
Identity = 403/576 (69.97%), Postives = 468/576 (81.25%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           M +   PP +QL+A  L +D ST +  KA+FSDRV IR+I+    GG+ LAG KVR+GGW
Sbjct: 1   MGDEIVPPANQLAADNLENDGSTVQ--KAQFSDRVLIRSIL---GGGAKLAGQKVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGR+  K  FAFLE+NDGSCP NLQV++++++ DL ++V TGTCV VDGV K+PP  K
Sbjct: 61  VKTGRQQGKGTFAFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGK 120

Query: 121 --HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q IEL  E V+ VG VDP  YPLPKTKLT EFLRD +HLR RTN ISAVARIRNALA
Sbjct: 121 GLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQVTTL++  ER+E++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IVKE+GEAV              XXXXXXX        +EER +LKPG+P+ DGK+
Sbjct: 241 AARLIVKERGEAVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVEERLRLKPGLPKNDGKI 300

Query: 301 DYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY  DFF RQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FAD+ DDMNCAEAYV+++C+WL+D C DDM+ M    D  C  RL +V+   F R +YTE
Sbjct: 361 FADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTE 420

Query: 421 AIEILEEAIKKGK-EFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRL 480
           AIE LE+A+ +GK  F+NKVEWGIDLASEHERYLTEV F +KP+IVYNYPKGIKAFYMRL
Sbjct: 421 AIERLEKAVAQGKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRL 480

Query: 481 NDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVK 540
           NDD KTVAAMDVLVPKVGELIGGSQREER++VI+ RI+E+GLP+EPYEWYLDLRRYGTVK
Sbjct: 481 NDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVK 540

Query: 541 HSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           H GFGLGFERM+ FATGIDNIRDVIPFPRYPG+ADL
Sbjct: 541 HCGFGLGFERMIQFATGIDNIRDVIPFPRYPGKADL 571

BLAST of Carg16490 vs. TAIR10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein)

HSP 1 Score: 486.9 bits (1252), Expect = 1.7e-137
Identity = 264/553 (47.74%), Postives = 342/553 (61.84%), Query Frame = 0

Query: 24  YKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGWVKTGRKADKDAFAFLELNDGSCP 83
           + S   EF  ++ I  +    D G    G  + + GWV+T R   + +  F+E+NDGSC 
Sbjct: 81  FGSKVGEFRKKLRIAEVKGGADEGLSRVGQSLNIMGWVRTLR--SQSSVTFIEINDGSCL 140

Query: 84  QNLQVIIEAAVADLGQI----VPTGTCVVVDGVFKLPPPDKHQKIELRAEAVVHVGPVDP 143
            NLQ ++ +      Q+    + TG  V V G        K QK+EL+ E ++ VG  D 
Sbjct: 141 SNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIVASQGTK-QKVELKVEKIIVVGECD- 200

Query: 144 ARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVT 203
           + YP+ K +++ EFLR   HLRPRTNT  AVAR+RN LAYATH FF + GF+++ +PI+T
Sbjct: 201 SSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQESGFVWVASPIIT 260

Query: 204 TSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEARKIVKEKGEAVSNLKASKA 263
            SDCEGAGE F VTTL+              P S    D +   + +             
Sbjct: 261 ASDCEGAGEQFCVTTLI--------------PSSHENTDTSIDAIPK------------- 320

Query: 264 SKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQV 323
                                             K G +D+ +DFF + AFLTVSGQL  
Sbjct: 321 ---------------------------------TKGGLIDWSQDFFGKPAFLTVSGQLNG 380

Query: 324 ETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQW 383
           ETYA ALS VYTFGPTFRAENS+TSRHLAEFWM+EPE+AFADL DDM CA AY++++ ++
Sbjct: 381 ETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACATAYLQYVVKY 440

Query: 384 LLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAIEILEEAIKKGKEFENKVEW 443
           +LDNC +DM+F     ++  I RL  V+   F++  YT+AIEIL   +K  K+F+  V+W
Sbjct: 441 VLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEIL---LKANKKFDFPVKW 500

Query: 444 GIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRLNDDSKTVAAMDVLVPKVGELIG 503
           G+DL SEHERY+TE  F  +PVI+ +YPK IKAFYMR NDD KTVAAMD+LVP++GELIG
Sbjct: 501 GLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMDMLVPRIGELIG 560

Query: 504 GSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIR 563
           GSQREER EV+  R+ E+ L  E Y WYLDLRRYG+V H+GFGLGFER++ F TGIDNIR
Sbjct: 561 GSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIR 566

Query: 564 DVIPFPRYPGRAD 572
           DVIPFPR P  A+
Sbjct: 621 DVIPFPRTPASAE 566

BLAST of Carg16490 vs. TAIR10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2)

HSP 1 Score: 475.7 bits (1223), Expect = 4.0e-134
Identity = 258/632 (40.82%), Postives = 374/632 (59.18%), Query Frame = 0

Query: 18  NDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGWVKTGRKA---------- 77
           ++D+S      +++S RV ++T++ R D G+GLAG +V +GGWVK+ R            
Sbjct: 13  DNDLSPKPITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKXXXXXXXXX 72

Query: 78  --------------------------------------------------DKDAFA---- 137
                                                             DK   A    
Sbjct: 73  XXXXXXXXXXGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKA 132

Query: 138 --------FLELNDGSCPQNLQVIIEAAVADL--GQIVPTGTCVVVDGVFKLP-PPDKHQ 197
                   +  ++DGS   +LQV++++A++ +   Q++  GTC+V +GV +LP       
Sbjct: 133 VPPPEYVFYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAKH 192

Query: 198 KIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYATH 257
            IEL AE ++HVG VDP +YPL K +L L  LRDF H RPRT T+ +V R+ +AL  A+H
Sbjct: 193 VIELEAEKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASH 252

Query: 258 TFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAE---VDE 317
           TF   HGF Y+  P++TT+   G GEMF+VTTLL + +  E+   K PP  E +   +D 
Sbjct: 253 TFLQYHGFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEE---KKPPVQEKDGFSIDT 312

Query: 318 ARKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKVD 377
            + ++KEK   + +LK S ++++ +        K  +  +++E + K K G   K  K+D
Sbjct: 313 VKAVIKEKTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLD 372

Query: 378 YGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAF 437
           + +DFF R  +LT SG+  +E+YA AL  VYTFGP F A+    +RHLAE W VE E+AF
Sbjct: 373 FSKDFFGRDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAF 432

Query: 438 ADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEA 497
           A+L D M+CA+ Y +FLC+++L+N  +DMKF++ + D+T   RL+  +S+  +R SYTE 
Sbjct: 433 AELDDAMDCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEV 492

Query: 498 IEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDD 557
           I +L++A     +FE K EWG+ L +EH  YLT+ ++K PVIV+ YPK IK FY+RLNDD
Sbjct: 493 ISLLQKATT--TKFETKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLNDD 552

Query: 558 SKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSG 572
            KTVAA D++VPKVG +I GSQ EERFE++  RI E G   E +EWYLDLRR+GTVKHSG
Sbjct: 553 KKTVAAFDLVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSG 612

BLAST of Carg16490 vs. TAIR10
Match: AT4G31180.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein)

HSP 1 Score: 119.0 bits (297), Expect = 9.5e-27
Identity = 91/291 (31.27%), Postives = 146/291 (50.17%), Query Frame = 0

Query: 286 KLKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQVETYACA-LSSVYTFGPTFRAENSHTS 345
           KL  G  E  G   +  ++  + A L  S QL  +   C  L  V+  GP FRAE+S T 
Sbjct: 279 KLMAGSSE-GGSAVFRLEYKGQPACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTH 338

Query: 346 RHLAEFWMVEPEIAF-ADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRL 405
           RHL EF  ++ E+       + M+  +    F+   L + C  +++ +  +Y     + L
Sbjct: 339 RHLCEFVGLDVEMEIRKHYSEIMDLVDELFVFIFTSLNERCKKELQAVGKQYP---FEPL 398

Query: 406 KLVSSTPFVRTSYTEAIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKP---- 465
           K +  T  +R ++ E +++L+EA   G E    V+   DL +E ER L ++V +K     
Sbjct: 399 KFLPKT--LRLTFEEGVQMLKEA---GVE----VDPLGDLNTESERKLGQLVLEKYNTEF 458

Query: 466 VIVYNYPKGIKAFY-MRLNDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGL 525
            I++ YPK ++ FY M   D+     + DV + +  E+I G+QR    EV+  R  E G+
Sbjct: 459 YILHRYPKAVRPFYTMTCADNPLYSNSFDVFI-RGEEIISGAQRVHIPEVLEQRAGECGI 518

Query: 526 PLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGR 570
            ++    Y+D  RYG   H GFG+G ER+++    ++NIR    FPR P R
Sbjct: 519 DVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALNNIRKTSLFPRDPQR 555

BLAST of Carg16490 vs. Swiss-Prot
Match: sp|Q9SW96|SYNC1_ARATH (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 9.1e-245
Identity = 409/574 (71.25%), Postives = 487/574 (84.84%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MA+   PP  QL+AV+L +D ST +  +A+FS+RV IRTI+ RPDGG+GLAG  VR+GGW
Sbjct: 1   MADEIVPPATQLAAVSLENDGSTVQ--RAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VK+GR   K  F+FL +NDGSCP NLQV+++ ++ D+  +V TGTCV VDGV K+PP  K
Sbjct: 61  VKSGRDQGKRTFSFLAVNDGSCPANLQVMVDPSLYDVSNLVATGTCVTVDGVLKVPPKGK 120

Query: 121 --HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q+IEL    V+ VG VD ++YPLPKTKLTLE LRD +HLR RTN+ISAVARIRNALA
Sbjct: 121 GTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQ TTL++  ERLE++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IV E+G  V+ LKA+KASK+ I         AKE  A ++ER++L+PG+P+KDG +
Sbjct: 241 AARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNI 300

Query: 301 DYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY +DFF RQAFLTVSGQLQVETYACALS+VYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FADL+DDMNCAEAYV+++C WLL+ C  DM+ MA  +D  CIDRLKLV+STPF R +YT+
Sbjct: 361 FADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTK 420

Query: 421 AIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 480
           AIE+LEEA+ KGKEF+N VEWGIDLASEHERYLTEV+F+KP+IVYNYPKGIKAFYMRLND
Sbjct: 421 AIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLND 480

Query: 481 DSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHS 540
           D KTVAAMDVLVPKVGELIGGSQREER++VI+ RI+E+GLP+EPYEWYLDLRRYGTVKH 
Sbjct: 481 DEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHC 540

Query: 541 GFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GFGLGFERM+LFATG+DNIRDVIPFPRYPG+ADL
Sbjct: 541 GFGLGFERMILFATGLDNIRDVIPFPRYPGKADL 572

BLAST of Carg16490 vs. Swiss-Prot
Match: sp|Q9SSK1|SYNC3_ARATH (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 5.4e-229
Identity = 403/576 (69.97%), Postives = 468/576 (81.25%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           M +   PP +QL+A  L +D ST +  KA+FSDRV IR+I+    GG+ LAG KVR+GGW
Sbjct: 1   MGDEIVPPANQLAADNLENDGSTVQ--KAQFSDRVLIRSIL---GGGAKLAGQKVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGR+  K  FAFLE+NDGSCP NLQV++++++ DL ++V TGTCV VDGV K+PP  K
Sbjct: 61  VKTGRQQGKGTFAFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGK 120

Query: 121 --HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q IEL  E V+ VG VDP  YPLPKTKLT EFLRD +HLR RTN ISAVARIRNALA
Sbjct: 121 GLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQVTTL++  ER+E++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IVKE+GEAV              XXXXXXX        +EER +LKPG+P+ DGK+
Sbjct: 241 AARLIVKERGEAVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVEERLRLKPGLPKNDGKI 300

Query: 301 DYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY  DFF RQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FAD+ DDMNCAEAYV+++C+WL+D C DDM+ M    D  C  RL +V+   F R +YTE
Sbjct: 361 FADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTE 420

Query: 421 AIEILEEAIKKGK-EFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRL 480
           AIE LE+A+ +GK  F+NKVEWGIDLASEHERYLTEV F +KP+IVYNYPKGIKAFYMRL
Sbjct: 421 AIERLEKAVAQGKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRL 480

Query: 481 NDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVK 540
           NDD KTVAAMDVLVPKVGELIGGSQREER++VI+ RI+E+GLP+EPYEWYLDLRRYGTVK
Sbjct: 481 NDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVK 540

Query: 541 HSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           H GFGLGFERM+ FATGIDNIRDVIPFPRYPG+ADL
Sbjct: 541 HCGFGLGFERMIQFATGIDNIRDVIPFPRYPGKADL 571

BLAST of Carg16490 vs. Swiss-Prot
Match: sp|Q6MEC9|SYN_PARUW (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.5e-143
Identity = 269/525 (51.24%), Postives = 335/525 (63.81%), Query Frame = 0

Query: 50  LAGHKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIV---PTGTC 109
           L GH + + GWV+T R  ++  F F+E+NDGS   N Q+I    +A   Q++    TG  
Sbjct: 18  LIGHDITLKGWVRTVR--NQKTFTFIEINDGSTLSNFQIIATPDIAGYDQLINQLSTGVS 77

Query: 110 VVVDGVFKLPPPDKHQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNT 169
           V   G   +  P K Q +E++A A+  +G  DP  Y L K + T EFLR   HLRPRTNT
Sbjct: 78  VSAIGTI-VESPGKEQNLEMQATAITIIGKCDPEVYLLQKKRHTFEFLRSIAHLRPRTNT 137

Query: 170 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 229
           I AV R+RNALA+ATH FF K GFLYIHTPI+T SDCEGAG+MFQVTTL           
Sbjct: 138 IGAVTRVRNALAFATHQFFQKRGFLYIHTPIITGSDCEGAGKMFQVTTL----------- 197

Query: 230 IKNPPPSEAEVDEARKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAK 289
                                                           + N AR  E   
Sbjct: 198 -----------------------------------------------DQNNPARTPE--- 257

Query: 290 LKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRH 349
                    G+VDY +DFF +  +LTVSGQL  E YACALS VYTFGPTFRAENS+TSRH
Sbjct: 258 ---------GRVDYTQDFFGKPTYLTVSGQLNGEIYACALSDVYTFGPTFRAENSNTSRH 317

Query: 350 LAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLV 409
           LAEFWM+EPE+AFADL D+M+CAE Y++++ +++LDNC +DM+F         I RL+ V
Sbjct: 318 LAEFWMIEPEMAFADLNDNMDCAEDYLKYILKYVLDNCQEDMEFFNKHVATDLISRLEHV 377

Query: 410 SSTPFVRTSYTEAIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 469
            +T F R SYT A+ ILE+A KK   FE  V+WG+DL SEHER+L E  F KPVI+ +YP
Sbjct: 378 INTSFERASYTYAVRILEKADKK---FEYPVKWGLDLQSEHERFLAEEFFGKPVILTDYP 437

Query: 470 KGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWY 529
           K IKAFYMR N+D+KTVAAMDVLVPKVGE+IGGSQREER  V+  ++KE  LP E Y WY
Sbjct: 438 KDIKAFYMRTNEDNKTVAAMDVLVPKVGEIIGGSQREERLSVLESKLKEFNLPAEEYWWY 466

Query: 530 LDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 572
           L+LR++G+V HSGFG GFER++ F TG++NIRDVIPFPR+PG+AD
Sbjct: 498 LELRKFGSVPHSGFGAGFERLVQFTTGMENIRDVIPFPRHPGKAD 466

BLAST of Carg16490 vs. Swiss-Prot
Match: sp|Q7MVE5|SYN_PORGI (Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=asnS PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 3.9e-139
Identity = 255/525 (48.57%), Postives = 327/525 (62.29%), Query Frame = 0

Query: 49  GLAGHKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQI--VPTGTC 108
           GL G ++ + GWV+T  K    A  F+ LNDGS   N+Q++ + A  D  Q+  + TG C
Sbjct: 17  GLVGQEINIKGWVRT--KRGNKAVNFIALNDGSTIHNMQIVADLASFDAAQMSQITTGAC 76

Query: 109 VVVDGVFKLPPPDKHQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNT 168
           + V G   +      Q +E++A A+   G  DPA YPL K   TLEFLR+  HLRPRTNT
Sbjct: 77  IGVIGTL-VESQGAGQSVEVQASAIEIYGTADPATYPLQKKGHTLEFLREIAHLRPRTNT 136

Query: 169 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 228
             A+ RIR+ +A A HTFF+  G+ Y H P++T SDCEGAG+MFQVTTL           
Sbjct: 137 FGAIYRIRHHMAIAIHTFFHNKGYYYFHAPLITASDCEGAGQMFQVTTL----------- 196

Query: 229 IKNPPPSEAEVDEARKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAK 288
             NP                                             +++ R EE   
Sbjct: 197 --NP---------------------------------------------DSLPRTEE--- 256

Query: 289 LKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRH 348
                    G+VDY +DFF R   LTVSGQL+ E  A AL  +YTFGPTFRAENS+T RH
Sbjct: 257 ---------GQVDYRKDFFGRHTSLTVSGQLEGEMAAMALGGIYTFGPTFRAENSNTPRH 316

Query: 349 LAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLV 408
           LAEFWM+EPE+AF +++D+M+ AE ++++  QW LDNC+DD+ F+++ +D+  IDRLK V
Sbjct: 317 LAEFWMIEPEVAFLEIEDNMDLAEEFIKYCVQWALDNCMDDISFLSEHFDKELIDRLKFV 376

Query: 409 SSTPFVRTSYTEAIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 468
              PFVR +YTE I ILEEA+K G +FE  + WG DLASEHERYL EV FK PVI+ +YP
Sbjct: 377 LEKPFVRLAYTEGIRILEEAVKNGVKFEFPIYWGADLASEHERYLVEVHFKTPVIMTDYP 436

Query: 469 KGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWY 528
           K IK+FYM+LNDD KTV  MDVL PK+GE+IGGS+RE  ++ +  R  E+G+P +   WY
Sbjct: 437 KEIKSFYMKLNDDGKTVRGMDVLFPKIGEIIGGSEREADYDKLVARANEMGVPEKDIWWY 468

Query: 529 LDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 572
           LD RRYGT  HSGFGLGFER+LLF TG+ NIRDVIPFPR P  A+
Sbjct: 497 LDSRRYGTAPHSGFGLGFERLLLFVTGMSNIRDVIPFPRTPNNAE 468

BLAST of Carg16490 vs. Swiss-Prot
Match: sp|B2RK42|SYN_PORG3 (Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561) OX=431947 GN=asnS PE=3 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 1.1e-138
Identity = 254/525 (48.38%), Postives = 326/525 (62.10%), Query Frame = 0

Query: 49  GLAGHKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQI--VPTGTC 108
           GL G ++ + GWV+T  K    A  F+ LNDGS   N+Q++ + A  D  Q+  + TG C
Sbjct: 17  GLVGQEINIKGWVRT--KRGNKAVNFIALNDGSTIHNMQIVADLASFDAAQMSQITTGAC 76

Query: 109 VVVDGVFKLPPPDKHQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNT 168
           + V G   +      Q +E++A A+   G  DPA YPL K   TLEFLR+  HLRPRTNT
Sbjct: 77  IGVIGTL-VESQGAGQSVEVQASAIEIYGTADPATYPLQKKGHTLEFLREIAHLRPRTNT 136

Query: 169 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 228
              + RIR+ +A A HTFF+  G+ Y H P++T SDCEGAG+MFQVTTL           
Sbjct: 137 FGVIYRIRHHMAIAIHTFFHNKGYYYFHAPLITASDCEGAGQMFQVTTL----------- 196

Query: 229 IKNPPPSEAEVDEARKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAK 288
             NP                                             +++ R EE   
Sbjct: 197 --NP---------------------------------------------DSLPRTEE--- 256

Query: 289 LKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRH 348
                    G+VDY +DFF R   LTVSGQL+ E  A AL  +YTFGPTFRAENS+T RH
Sbjct: 257 ---------GQVDYRKDFFGRHTSLTVSGQLEGEMAAMALGGIYTFGPTFRAENSNTPRH 316

Query: 349 LAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLV 408
           LAEFWM+EPE+AF +++D+M+ AE ++++  QW LDNC+DD+ F+++ +D+  IDRLK V
Sbjct: 317 LAEFWMIEPEVAFLEIEDNMDLAEEFIKYCVQWALDNCMDDISFLSEHFDKELIDRLKFV 376

Query: 409 SSTPFVRTSYTEAIEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 468
              PFVR +YTE I ILEEA+K G +FE  + WG DLASEHERYL EV FK PVI+ +YP
Sbjct: 377 LEKPFVRLAYTEGIRILEEAVKNGVKFEFPIYWGADLASEHERYLVEVHFKTPVIMTDYP 436

Query: 469 KGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWY 528
           K IK+FYM+LNDD KTV  MDVL PK+GE+IGGS+RE  ++ +  R  E+G+P +   WY
Sbjct: 437 KEIKSFYMKLNDDGKTVRGMDVLFPKIGEIIGGSEREADYDKLVARANEMGVPEKDIWWY 468

Query: 529 LDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 572
           LD RRYGT  HSGFGLGFER+LLF TG+ NIRDVIPFPR P  A+
Sbjct: 497 LDSRRYGTAPHSGFGLGFERLLLFVTGMSNIRDVIPFPRTPNNAE 468

BLAST of Carg16490 vs. TrEMBL
Match: tr|A0A1S3BPW6|A0A1S3BPW6_CUCME (asparagine--tRNA ligase, cytoplasmic 1-like OS=Cucumis melo OX=3656 GN=LOC103492241 PE=3 SV=1)

HSP 1 Score: 1058.1 bits (2735), Expect = 6.9e-306
Identity = 528/572 (92.31%), Postives = 548/572 (95.80%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPP DQL+AV LN+D S Y+SLKAEFSDRVPIRTIV RPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPTDQLAAVTLNNDASAYESLKAEFSDRVPIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA+VADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRA+AVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFN HGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R IVKEKGEAV++LKA+K       XXXXXXX   ENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RNIVKEKGEAVASLKAAKXXXXXXXXXXXXXXXXXENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRF+CQWLLDNCLDDMKFMA+KYDRTCIDRLK+VSSTPFVR SYTEA+
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCLDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           E+LEEA+KKGKEFENKVEWGIDLASEHERYLTEVVF+KPVIVYNYPKGIKAFYMRLNDD 
Sbjct: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFRKPVIVYNYPKGIKAFYMRLNDDL 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+GLPLEPYEWYLDLRRYGTVKH GF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

BLAST of Carg16490 vs. TrEMBL
Match: tr|A0A0A0LYN5|A0A0A0LYN5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G573730 PE=3 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 1.0e-301
Identity = 523/572 (91.43%), Postives = 540/572 (94.41%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MAEHSAPP DQL+AVALN+D   Y+SLKAEFSDRV IRTIV RPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPTDQLAAVALNNDAPAYESLKAEFSDRVTIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA+VADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRA+AVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFN HGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAV              XXXXXXX    NVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVXXXXXXXXXXXXXXXXXXXXXXXXXNVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           DLQDDMNCAEAYVRF+CQWLLDNC+DDMKFMA+KYDRTCIDRLK+VSSTPFVR SYTEA+
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCIDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           E+LEEA+KKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDD 
Sbjct: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDM 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+GLPLEPYEWYLDLRRYGTVKH GF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

BLAST of Carg16490 vs. TrEMBL
Match: tr|A0A2I4GR02|A0A2I4GR02_9ROSI (asparagine--tRNA ligase, cytoplasmic 1 isoform X1 OS=Juglans regia OX=51240 GN=LOC109010069 PE=3 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 1.8e-277
Identity = 460/572 (80.42%), Postives = 523/572 (91.43%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MA+ SA    QL+A  L+D VS+ +  KAEFSDRVPIR+IV RPDGG+GLAGH+VR+GGW
Sbjct: 1   MADDSASLSGQLAATTLHDAVSSSEVAKAEFSDRVPIRSIVSRPDGGAGLAGHRVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCP NLQVI++A+V+DL  +VPTGTC+ VDGV KLPP   
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPANLQVIVDASVSDLSPLVPTGTCISVDGVLKLPPSGT 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
            QK+ELR E VVHVGPVDPA+YPLPKT+LTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 KQKVELRVEKVVHVGPVDPAKYPLPKTRLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFF KHGFLY+HTPI+TTSDCEGAGEMFQVTTL+SEA++LEK+LIKNPPPS A+++ A
Sbjct: 181 THTFFQKHGFLYVHTPIITTSDCEGAGEMFQVTTLISEADKLEKDLIKNPPPSAADIEAA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKVDY 300
           + +VKEKGE V+ LK++KA K++I        K KEN+++LEER+KLKPGIP+KDGK+DY
Sbjct: 241 QLLVKEKGEVVAQLKSAKAKKEDINVSVAELKKEKENLSKLEERSKLKPGIPQKDGKIDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           G DFF RQAFLTVSGQLQVE+YAC LSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GLDFFGRQAFLTVSGQLQVESYACCLSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           +L+DDMNCAEAYVRFLCQWLLDNCLDDM+FMADK+D+TCI+RL++V+STPF R SYTEA+
Sbjct: 361 ELKDDMNCAEAYVRFLCQWLLDNCLDDMEFMADKFDKTCIERLRMVASTPFERISYTEAV 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           E+LEEA+K G++FENKVEWGIDLASEHERYLTEV F+KPVIVYNYPK IKAFYMRLNDDS
Sbjct: 421 ELLEEAVKGGRKFENKVEWGIDLASEHERYLTEVKFQKPVIVYNYPKDIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER++VI+ RIK++GLP+EPYEWYLDLRRYGTVKH GF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYDVIQQRIKDMGLPIEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGI+NIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIENIRDVIPFPRYPGRADL 572

BLAST of Carg16490 vs. TrEMBL
Match: tr|V7CQ72|V7CQ72_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_002G305800g PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 9.4e-271
Identity = 456/573 (79.58%), Postives = 520/573 (90.75%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           M + S+PP DQL+A  L+D+V      KA+FSDRVPIR+I+ R DGGSGLAG K RVGGW
Sbjct: 1   MTDTSSPPTDQLAAATLDDEVP-----KAQFSDRVPIRSIISRSDGGSGLAGRKARVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLE+NDGSC  NLQVI+EA++ +LGQ+VPTGTCVVVDG  KLPP   
Sbjct: 61  VKTGRKADKDAFAFLEINDGSCAGNLQVIVEASLGELGQLVPTGTCVVVDGHLKLPPAGT 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
            QKIELRA+ V+HVGPVDPA+YPLPK +LTLEFLRDFVHLR RTNTISAVARIRNALAYA
Sbjct: 121 KQKIELRADKVLHVGPVDPAKYPLPKMRLTLEFLRDFVHLRSRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNK GFLY+HTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL +NPPP+EA+V+ A
Sbjct: 181 THTFFNKEGFLYVHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELSQNPPPTEADVEAA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEK-DGKVD 300
           R +V+ KG+ V+ LKA+KA+K EIG       KAKE++A++EER+KLKPGIP+K DGKVD
Sbjct: 241 RFVVQGKGDVVAQLKAAKATKPEIGAAVDELKKAKESLAKVEERSKLKPGIPKKDDGKVD 300

Query: 301 YGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAF 360
           YG+DFFARQAFLTVSGQLQVE+Y CALSSVYTFGPTFRAENSHTSRHLAEFWMVEPE+AF
Sbjct: 301 YGKDFFARQAFLTVSGQLQVESYVCALSSVYTFGPTFRAENSHTSRHLAEFWMVEPELAF 360

Query: 361 ADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEA 420
           A+L+DDMNCAEAYV+F+CQWLLDNCL+DM+FMADK+D+ CIDRLKLV+ +PF+R SYTEA
Sbjct: 361 AELKDDMNCAEAYVKFMCQWLLDNCLEDMEFMADKFDKGCIDRLKLVAFSPFIRVSYTEA 420

Query: 421 IEILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDD 480
           +EILE+A+K GK+FEN+V+WGIDLASEHER+LTEV F+KPVIVYNYPK IKAFYMRLNDD
Sbjct: 421 VEILEDAVKNGKKFENEVKWGIDLASEHERFLTEVKFQKPVIVYNYPKEIKAFYMRLNDD 480

Query: 481 SKTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSG 540
            KTVAAMDVLVPKVGELIGGSQREER++VI  RIKE+GLPLEPYEWYLDLRRYGTVKH+G
Sbjct: 481 LKTVAAMDVLVPKVGELIGGSQREERYDVIESRIKEMGLPLEPYEWYLDLRRYGTVKHAG 540

Query: 541 FGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           FGLGFERM+LFATG++NIRDVIPFPRYPGRADL
Sbjct: 541 FGLGFERMILFATGLENIRDVIPFPRYPGRADL 568

BLAST of Carg16490 vs. TrEMBL
Match: tr|W9QBZ5|W9QBZ5_9ROSA (Asparagine--tRNA ligase, cytoplasmic 1 OS=Morus notabilis OX=981085 GN=L484_005478 PE=3 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 1.6e-270
Identity = 462/572 (80.77%), Postives = 514/572 (89.86%), Query Frame = 0

Query: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60
           MA+ S PP  QLS   LND VS+  SL+AEFSDRVPIR+I+ RPDGG+GLAG++VRVGGW
Sbjct: 1   MADDSTPPTAQLSQTTLNDAVSSSDSLRAEFSDRVPIRSILSRPDGGAGLAGNRVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCP NLQVI+EA  ADLGQ+VPTGTCV VDGV KLPP   
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPGNLQVIVEADKADLGQLVPTGTCVAVDGVLKLPPAGT 120

Query: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
            QK+ELR E VVHVGPVDPA+YPLPKT+LTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 KQKVELRVEKVVHVGPVDPAKYPLPKTRLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFF KHGFLY+HTPI+TTSDCEGAGEMFQVTTL+SEAERLEK+LIKNPPP+E++++ A
Sbjct: 181 THTFFQKHGFLYVHTPIITTSDCEGAGEMFQVTTLISEAERLEKDLIKNPPPTESDLEAA 240

Query: 241 RKIVKEKGEAVSNLKASKASKQEIGXXXXXXXKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R +VKEKG+                XXXXXXX     +A+LEER+KLKPGIP+KDGK+DY
Sbjct: 241 RLVVKEKGDFXXXXXXXXXXXXXXXXXXXXXXXXXXXLAKLEERSKLKPGIPQKDGKIDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVE++ C+LSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 TQDFFARQAFLTVSGQLQVESFVCSLSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420
           +L+DDM CAEAYVRFLCQWLLDNC DDM+FMADK+D+ CI+RLKLVSSTPF R +YT+A+
Sbjct: 361 ELEDDMKCAEAYVRFLCQWLLDNCYDDMEFMADKFDKGCINRLKLVSSTPFERITYTKAV 420

Query: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           E+LEEA+K GK FENKVEWGIDLASEHERYLTEV+F+KPVIVYNYPKGIKAFYMRLNDD 
Sbjct: 421 ELLEEAVKNGKVFENKVEWGIDLASEHERYLTEVMFQKPVIVYNYPKGIKAFYMRLNDDG 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER++VI+ RI+E+GLP EPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYDVIQSRIQEMGLPTEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERM+LFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMILFATGIDNIRDVIPFPRYPGRADL 572