Carg16457 (gene) Silver-seed gourd

BLAST of Carg16457 vs. NCBI nr
Match: XP_022931853.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 3472.6 bits (9003), Expect = 0.0e+00
Identity = 1775/1778 (99.83%), Postives = 1777/1778 (99.94%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120
            CGRVFCSKCTANSI APSTDPTNSREDSEKIRVCNYCFKQWGKEPSV+QDGDKATCSAVS
Sbjct: 61   CGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVMQDGDKATCSAVS 120

Query: 121  LSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINXX 180
            LSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINXX
Sbjct: 121  LSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINXX 180

Query: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNADEGLKLDGEDI 240
            XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNADEGLKLDGEDI
Sbjct: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNADEGLKLDGEDI 240

Query: 241  SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX 300
            SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX
Sbjct: 241  SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX 300

Query: 301  XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR 360
            XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR
Sbjct: 301  XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR 360

Query: 361  ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA 420
            ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA
Sbjct: 361  ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA 420

Query: 421  GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL 480
            GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL
Sbjct: 421  GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL 480

Query: 481  DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII 540
            DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII
Sbjct: 481  DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII 540

Query: 541  PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660
            CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI
Sbjct: 601  CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660

Query: 661  SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSNASSTS 720
            SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSNASSTS
Sbjct: 661  SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSNASSTS 720

Query: 721  LSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYISSNALD 780
            LSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYISSNALD
Sbjct: 721  LSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYISSNALD 780

Query: 781  GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH 840
            GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH
Sbjct: 781  GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH 840

Query: 841  LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF 900
            LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF
Sbjct: 841  LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF 900

Query: 901  FLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV 960
            FLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV
Sbjct: 901  FLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV 960

Query: 961  AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHN 1020
            AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHN
Sbjct: 961  AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHN 1020

Query: 1021 RAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLS 1080
            RAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLS
Sbjct: 1021 RAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLS 1080

Query: 1081 GETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI 1140
            GETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI
Sbjct: 1081 GETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI 1140

Query: 1141 ESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL 1200
            ESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL
Sbjct: 1141 ESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL 1200

Query: 1201 GVLEDDLCLSPVMPQDVEISMRRANSEGEYPIMADLSDTLDAVWKGVMHPNSATSDSSYS 1260
            GVLEDDLCLSPVMPQDVEISMRRANSEG+YPIMADLSDTLDAVWKGVMHPNSATSDSSYS
Sbjct: 1201 GVLEDDLCLSPVMPQDVEISMRRANSEGDYPIMADLSDTLDAVWKGVMHPNSATSDSSYS 1260

Query: 1261 RTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNT 1320
            RTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNT
Sbjct: 1261 RTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNT 1320

Query: 1321 KISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSL 1380
            KISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSL
Sbjct: 1321 KISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSL 1380

Query: 1381 LTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSM 1440
            LTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSM
Sbjct: 1381 LTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSM 1440

Query: 1441 PRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYV 1500
            PRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYV
Sbjct: 1441 PRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYV 1500

Query: 1501 RSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGC 1560
            RSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGC
Sbjct: 1501 RSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGC 1560

Query: 1561 PTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSG 1620
            PTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSG
Sbjct: 1561 PTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSG 1620

Query: 1621 KNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQE 1680
            KNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQE
Sbjct: 1621 KNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQE 1680

Query: 1681 LVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVP 1740
            LVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVP
Sbjct: 1681 LVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVP 1740

Query: 1741 DQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1779
            DQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD
Sbjct: 1741 DQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1778

BLAST of Carg16457 vs. NCBI nr
Match: XP_023520487.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3413.2 bits (8849), Expect = 0.0e+00
Identity = 1747/1778 (98.26%), Postives = 1753/1778 (98.59%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCY+CDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120
            CGRVFCSKCTANSI APSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS
Sbjct: 61   CGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120

Query: 121  LSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINXX 180
            LSSSSTSIGSS+SDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRN KSIN X
Sbjct: 121  LSSSSTSIGSSKSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNGKSINRX 180

Query: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNADEGLKLDGEDI 240
            XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSE R+ISQSDNYYCDVNADEGLKLDGEDI
Sbjct: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSECRNISQSDNYYCDVNADEGLKLDGEDI 240

Query: 241  SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX 300
            SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX
Sbjct: 241  SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX 300

Query: 301  XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR 360
            XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR
Sbjct: 301  XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR 360

Query: 361  ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA 420
            ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA
Sbjct: 361  ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA 420

Query: 421  GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL 480
            GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL
Sbjct: 421  GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL 480

Query: 481  DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII 540
            DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII
Sbjct: 481  DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII 540

Query: 541  PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660
            CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI
Sbjct: 601  CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660

Query: 661  SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSNASSTS 720
            SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSNASS  
Sbjct: 661  SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSNASSXX 720

Query: 721  LSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYISSNALD 780
                             SKFTSEANI LDNKDDDTVPISGKGEVSSQLRNSIYISSNALD
Sbjct: 721  XXXXXXXXXXXXXXXXXSKFTSEANIRLDNKDDDTVPISGKGEVSSQLRNSIYISSNALD 780

Query: 781  GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH 840
            GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH
Sbjct: 781  GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH 840

Query: 841  LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF 900
            LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF
Sbjct: 841  LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF 900

Query: 901  FLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV 960
            FLPGE EGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV
Sbjct: 901  FLPGESEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV 960

Query: 961  AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHN 1020
            AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHN
Sbjct: 961  AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHN 1020

Query: 1021 RAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLS 1080
            RAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLS
Sbjct: 1021 RAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLS 1080

Query: 1081 GETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI 1140
            GETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI
Sbjct: 1081 GETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI 1140

Query: 1141 ESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL 1200
            ESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL
Sbjct: 1141 ESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL 1200

Query: 1201 GVLEDDLCLSPVMPQDVEISMRRANSEGEYPIMADLSDTLDAVWKGVMHPNSATSDSSYS 1260
            GVLEDDLCLSPVMPQDVEISMRRANSEGEYPIMADLSDTLDAVWKGVMHPNSATSDSSYS
Sbjct: 1201 GVLEDDLCLSPVMPQDVEISMRRANSEGEYPIMADLSDTLDAVWKGVMHPNSATSDSSYS 1260

Query: 1261 RTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNT 1320
            RTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNT
Sbjct: 1261 RTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNT 1320

Query: 1321 KISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSL 1380
            KISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSL
Sbjct: 1321 KISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSL 1380

Query: 1381 LTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSM 1440
            LTPEYNAQM+EPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSM
Sbjct: 1381 LTPEYNAQMAEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSM 1440

Query: 1441 PRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYV 1500
            PRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYV
Sbjct: 1441 PRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYV 1500

Query: 1501 RSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGC 1560
            RSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGC
Sbjct: 1501 RSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGC 1560

Query: 1561 PTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSG 1620
            PTCLAKVVGMYQVSSKHPKGG+ECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSG
Sbjct: 1561 PTCLAKVVGMYQVSSKHPKGGKECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSG 1620

Query: 1621 KNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQE 1680
            KNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQE
Sbjct: 1621 KNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQE 1680

Query: 1681 LVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVP 1740
            LVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVP
Sbjct: 1681 LVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVP 1740

Query: 1741 DQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1779
            DQWPSSTVSPSESHPDLCEE+SEPEHSWDGKPYRYQSD
Sbjct: 1741 DQWPSSTVSPSESHPDLCEESSEPEHSWDGKPYRYQSD 1778

BLAST of Carg16457 vs. NCBI nr
Match: XP_023004649.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like [Cucurbita maxima])

HSP 1 Score: 3384.0 bits (8773), Expect = 0.0e+00
Identity = 1736/1779 (97.58%), Postives = 1749/1779 (98.31%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCY+CDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120
            CGRVFCSKCTANSI APSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS
Sbjct: 61   CGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120

Query: 121  LSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINXX 180
             SSSSTSIGS++SDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRN KSIN  
Sbjct: 121  PSSSSTSIGSTKSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNGKSINRT 180

Query: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNADEGLKLDGEDI 240
            XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCD NADEGLKLDGEDI
Sbjct: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDANADEGLKLDGEDI 240

Query: 241  SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX 300
            SKKGSPV+FDNQC RDSEESS      DKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX
Sbjct: 241  SKKGSPVIFDNQCQRDSEESSXXXXXXDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX 300

Query: 301  XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR 360
            XXXXXXXXXXXXXXXXXXX APGEWGYVPSSLD GECPVKG SSEEHRKTAKNVVEGHFR
Sbjct: 301  XXXXXXXXXXXXXXXXXXXCAPGEWGYVPSSLDIGECPVKGSSSEEHRKTAKNVVEGHFR 360

Query: 361  ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA 420
            ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA
Sbjct: 361  ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA 420

Query: 421  GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL 480
            GGKR ESIIVKGIICKKNVAHRRMTREISKP FFVLGGALEYQRVTNHLSSLDTLLQQEL
Sbjct: 421  GGKRCESIIVKGIICKKNVAHRRMTREISKPRFFVLGGALEYQRVTNHLSSLDTLLQQEL 480

Query: 481  DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII 540
            DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII
Sbjct: 481  DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII 540

Query: 541  PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKK+TKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKSTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660
            CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVAL HNPSNIDRSI
Sbjct: 601  CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALLHNPSNIDRSI 660

Query: 661  SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSNASSTS 720
            SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDS IISNNVVNLNSLRLLKSNASSTS
Sbjct: 661  SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSPIISNNVVNLNSLRLLKSNASSTS 720

Query: 721  LSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYISSNALD 780
            LSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYISSNALD
Sbjct: 721  LSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYISSNALD 780

Query: 781  GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH 840
            GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH
Sbjct: 781  GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH 840

Query: 841  LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF 900
            LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF
Sbjct: 841  LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF 900

Query: 901  FLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV 960
            FLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV
Sbjct: 901  FLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV 960

Query: 961  AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHN 1020
            AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPP+LIEFYHNNLEWINKEANEVHN
Sbjct: 961  AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPALIEFYHNNLEWINKEANEVHN 1020

Query: 1021 RAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLS 1080
            RAETLFA VCKALPLIPK+ELGSEQED GVNELNHYIQELEQIIIMEKEDFEVHFNEVLS
Sbjct: 1021 RAETLFAAVCKALPLIPKNELGSEQEDVGVNELNHYIQELEQIIIMEKEDFEVHFNEVLS 1080

Query: 1081 GETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI 1140
            GETTV QPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI
Sbjct: 1081 GETTVSQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI 1140

Query: 1141 ESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL 1200
            ESVEEFV+FTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNE EREK+IDL
Sbjct: 1141 ESVEEFVKFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNEMEREKNIDL 1200

Query: 1201 GVLEDDLCLSPVMPQDVEISMRRANSE-GEYPIMADLSDTLDAVWKGVMHPNSATSDSSY 1260
            GVLEDDLCLSPVMPQDVEISMRRANSE GEYPIMADLSDTLDAVWKGVMHPNS TS+SSY
Sbjct: 1201 GVLEDDLCLSPVMPQDVEISMRRANSEGGEYPIMADLSDTLDAVWKGVMHPNSVTSESSY 1260

Query: 1261 SRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYN 1320
            S+TNSAILEPLV QSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAY+
Sbjct: 1261 SQTNSAILEPLVVQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYS 1320

Query: 1321 TKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYS 1380
            TKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGS DTVVPVYDEEPTSIIAYS
Sbjct: 1321 TKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSNDTVVPVYDEEPTSIIAYS 1380

Query: 1381 LLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILS 1440
            LLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILS
Sbjct: 1381 LLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILS 1440

Query: 1441 MPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDY 1500
            MPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDY
Sbjct: 1441 MPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDY 1500

Query: 1501 VRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTG 1560
            VRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTG
Sbjct: 1501 VRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTG 1560

Query: 1561 CPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTS 1620
            CPTCLAKVVGMYQVSSKH KGG+ECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTS
Sbjct: 1561 CPTCLAKVVGMYQVSSKHLKGGKECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTS 1620

Query: 1621 GKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQ 1680
            GKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQ
Sbjct: 1621 GKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQ 1680

Query: 1681 ELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMV 1740
            ELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMV
Sbjct: 1681 ELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMV 1740

Query: 1741 PDQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1779
            PDQWPSSTVSPSESHPDLCEENSEPEHSWDGK YRYQSD
Sbjct: 1741 PDQWPSSTVSPSESHPDLCEENSEPEHSWDGKRYRYQSD 1779

BLAST of Carg16457 vs. NCBI nr
Match: XP_022931861.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 3378.6 bits (8759), Expect = 0.0e+00
Identity = 1731/1778 (97.36%), Postives = 1734/1778 (97.53%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120
            CGRVFCSKCTANSI APSTDPTNSREDSEKIRVCNYCFKQWGKEPSV+QDGDKATCSAVS
Sbjct: 61   CGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVMQDGDKATCSAVS 120

Query: 121  LSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINXX 180
            LSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINXX
Sbjct: 121  LSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINXX 180

Query: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNADEGLKLDGEDI 240
            XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNADEGLKLDGEDI
Sbjct: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNADEGLKLDGEDI 240

Query: 241  SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX 300
            SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX
Sbjct: 241  SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX 300

Query: 301  XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR 360
            XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR
Sbjct: 301  XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR 360

Query: 361  ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA 420
            ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA
Sbjct: 361  ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA 420

Query: 421  GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL 480
            GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL
Sbjct: 421  GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL 480

Query: 481  DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII 540
            DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII
Sbjct: 481  DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII 540

Query: 541  PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660
            CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI
Sbjct: 601  CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660

Query: 661  SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSNASSTS 720
            SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSII                     
Sbjct: 661  SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSII--------------------- 720

Query: 721  LSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYISSNALD 780
                                  +ANISLDNKDDDTVPISGKGEVSSQLRNSIYISSNALD
Sbjct: 721  ----------------------KANISLDNKDDDTVPISGKGEVSSQLRNSIYISSNALD 780

Query: 781  GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH 840
            GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH
Sbjct: 781  GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH 840

Query: 841  LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF 900
            LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF
Sbjct: 841  LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF 900

Query: 901  FLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV 960
            FLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV
Sbjct: 901  FLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV 960

Query: 961  AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHN 1020
            AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHN
Sbjct: 961  AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHN 1020

Query: 1021 RAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLS 1080
            RAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLS
Sbjct: 1021 RAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLS 1080

Query: 1081 GETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI 1140
            GETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI
Sbjct: 1081 GETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI 1140

Query: 1141 ESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL 1200
            ESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL
Sbjct: 1141 ESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL 1200

Query: 1201 GVLEDDLCLSPVMPQDVEISMRRANSEGEYPIMADLSDTLDAVWKGVMHPNSATSDSSYS 1260
            GVLEDDLCLSPVMPQDVEISMRRANSEG+YPIMADLSDTLDAVWKGVMHPNSATSDSSYS
Sbjct: 1201 GVLEDDLCLSPVMPQDVEISMRRANSEGDYPIMADLSDTLDAVWKGVMHPNSATSDSSYS 1260

Query: 1261 RTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNT 1320
            RTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNT
Sbjct: 1261 RTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNT 1320

Query: 1321 KISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSL 1380
            KISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSL
Sbjct: 1321 KISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSL 1380

Query: 1381 LTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSM 1440
            LTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSM
Sbjct: 1381 LTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSM 1440

Query: 1441 PRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYV 1500
            PRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYV
Sbjct: 1441 PRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYV 1500

Query: 1501 RSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGC 1560
            RSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGC
Sbjct: 1501 RSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGC 1560

Query: 1561 PTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSG 1620
            PTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSG
Sbjct: 1561 PTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSG 1620

Query: 1621 KNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQE 1680
            KNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQE
Sbjct: 1621 KNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQE 1680

Query: 1681 LVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVP 1740
            LVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVP
Sbjct: 1681 LVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVP 1735

Query: 1741 DQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1779
            DQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD
Sbjct: 1741 DQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1735

BLAST of Carg16457 vs. NCBI nr
Match: XP_023520488.1 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3361.2 bits (8714), Expect = 0.0e+00
Identity = 1722/1778 (96.85%), Postives = 1729/1778 (97.24%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCY+CDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120
            CGRVFCSKCTANSI APSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS
Sbjct: 61   CGRVFCSKCTANSIPAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120

Query: 121  LSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINXX 180
            LSSSSTSIGSS+SDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRN KSIN X
Sbjct: 121  LSSSSTSIGSSKSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNGKSINRX 180

Query: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNADEGLKLDGEDI 240
            XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSE R+ISQSDNYYCDVNADEGLKLDGEDI
Sbjct: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSECRNISQSDNYYCDVNADEGLKLDGEDI 240

Query: 241  SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX 300
            SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX
Sbjct: 241  SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX 300

Query: 301  XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR 360
            XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR
Sbjct: 301  XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR 360

Query: 361  ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA 420
            ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA
Sbjct: 361  ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA 420

Query: 421  GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL 480
            GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL
Sbjct: 421  GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL 480

Query: 481  DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII 540
            DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII
Sbjct: 481  DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII 540

Query: 541  PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660
            CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI
Sbjct: 601  CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660

Query: 661  SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLRLLKSNASSTS 720
            SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSII                     
Sbjct: 661  SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSII--------------------- 720

Query: 721  LSFLKQDVSISDNNMLSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYISSNALD 780
                                  +ANI LDNKDDDTVPISGKGEVSSQLRNSIYISSNALD
Sbjct: 721  ----------------------KANIRLDNKDDDTVPISGKGEVSSQLRNSIYISSNALD 780

Query: 781  GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH 840
            GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH
Sbjct: 781  GNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAH 840

Query: 841  LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF 900
            LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF
Sbjct: 841  LLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDF 900

Query: 901  FLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV 960
            FLPGE EGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV
Sbjct: 901  FLPGESEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRV 960

Query: 961  AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHN 1020
            AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHN
Sbjct: 961  AGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHN 1020

Query: 1021 RAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLS 1080
            RAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLS
Sbjct: 1021 RAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLS 1080

Query: 1081 GETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI 1140
            GETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI
Sbjct: 1081 GETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGI 1140

Query: 1141 ESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL 1200
            ESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL
Sbjct: 1141 ESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL 1200

Query: 1201 GVLEDDLCLSPVMPQDVEISMRRANSEGEYPIMADLSDTLDAVWKGVMHPNSATSDSSYS 1260
            GVLEDDLCLSPVMPQDVEISMRRANSEGEYPIMADLSDTLDAVWKGVMHPNSATSDSSYS
Sbjct: 1201 GVLEDDLCLSPVMPQDVEISMRRANSEGEYPIMADLSDTLDAVWKGVMHPNSATSDSSYS 1260

Query: 1261 RTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNT 1320
            RTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNT
Sbjct: 1261 RTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNT 1320

Query: 1321 KISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSL 1380
            KISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSL
Sbjct: 1321 KISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSL 1380

Query: 1381 LTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSM 1440
            LTPEYNAQM+EPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSM
Sbjct: 1381 LTPEYNAQMAEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSM 1440

Query: 1441 PRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYV 1500
            PRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYV
Sbjct: 1441 PRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYV 1500

Query: 1501 RSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGC 1560
            RSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGC
Sbjct: 1501 RSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGC 1560

Query: 1561 PTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSG 1620
            PTCLAKVVGMYQVSSKHPKGG+ECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSG
Sbjct: 1561 PTCLAKVVGMYQVSSKHPKGGKECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSG 1620

Query: 1621 KNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQE 1680
            KNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQE
Sbjct: 1621 KNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQE 1680

Query: 1681 LVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVP 1740
            LVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVP
Sbjct: 1681 LVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVP 1735

Query: 1741 DQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1779
            DQWPSSTVSPSESHPDLCEE+SEPEHSWDGKPYRYQSD
Sbjct: 1741 DQWPSSTVSPSESHPDLCEESSEPEHSWDGKPYRYQSD 1735

BLAST of Carg16457 vs. TAIR10
Match: AT3G14270.1 (phosphatidylinositol-4-phosphate 5-kinase family protein)

HSP 1 Score: 1562.4 bits (4044), Expect = 0.0e+00
Identity = 913/1847 (49.43%), Postives = 1166/1847 (63.13%), Query Frame = 0

Query: 9    SDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRRCGRVFCSK 68
            S++VGL+KSW+PWRS+P  VSRDFWMPD+SCRVCY+CD QFT INRRHHCR CGRVFC K
Sbjct: 12   SEIVGLIKSWLPWRSEPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCGK 71

Query: 69   CTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGD----KATCSAVSLSSS 128
            CTANSI    +D    RED E+IRVCNYCF+QW       + GD     +  + +S S S
Sbjct: 72   CTANSIPFAPSDLRTPREDWERIRVCNYCFRQW-------EQGDGGPHVSNITELSTSPS 131

Query: 129  STSIGSSRSDYTCHSAISNVNSTP---CSNDRQYY--DPYSSGVITDEQDNLRNRKSI-- 188
             TS+ SS++  T +S+   + S P     N R ++  D    GV + E    +  K    
Sbjct: 132  ETSLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTKQGKETSR 191

Query: 189  -NXXXXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNADEGLKLD 248
             +                             Y++D E+ H  +++ YY  +  + G+ +D
Sbjct: 192  RSSFIATDVEDPSRFALNSIRSDDEYDEYGAYQTDIETSHSPRANEYYGPMEYN-GMGID 251

Query: 249  --------GEDISKK---GSPVMFDNQC----PRDSEESSEDSDENDKGSECD--FPTYA 308
                    GE   +K   GSP++  +QC     R+  E  +   E+D   EC+   P   
Sbjct: 252  DVPCKHLGGETADQKSLSGSPLI--HQCLESLIREGSEQFQKKSEHDGRDECEASSPADI 311

Query: 309  MQNTNNEAMDFQNNGILWLP--XXXXXXXXXXXXXXXXXXXXXAPGEWGYV--PSSLDSG 368
              +   E +DF+NNG+LW+P                       A GEWGY+   +S  SG
Sbjct: 312  SDDQVVEPVDFENNGLLWVPPEPENEEDERESALFDEEDNEGDASGEWGYLRPSTSFGSG 371

Query: 369  ECPVKGRSSEEHRKTAKNVVEGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAA 428
            E   + R++EEH+K  KNVV+GHFRAL++QLL+ EN+ + DE  ++ WL+IIT LSWEAA
Sbjct: 372  EYRGEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAA 431

Query: 429  AVLKPDTSKSGGMDPGGYVKVKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFFV 488
             +LKPD SKSGGMDPGGYVKVK +A G R +S++VKG++CKKNV +RRM+ +I K    +
Sbjct: 432  NLLKPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLI 491

Query: 489  LGGALEYQRVTNHLSSLDTLLQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSK 548
            LGG LEYQRV+N LSS DTLLQQE DHLKMAVAKI +  PN+LLVEKSVSR AQEYLL+K
Sbjct: 492  LGGGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAK 551

Query: 549  NITLVLNIKRSLLERIACCTGASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGK 608
            +I+LVLNIKR LL+RIA CTGA IIP+VDH++S KLGYC+ FRV+ + EEH S  GQ GK
Sbjct: 552  DISLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGS-TGQVGK 611

Query: 609  KTTKTLMFFEGCPKPLCCTILLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGAS 668
            K  KTLM+FE CPKPL  TILLRGA  DELKKVKHVVQY  FAAYHLALET FLADEGAS
Sbjct: 612  KVVKTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGAS 671

Query: 669  VPELPLNSPITVALPHNPSNIDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRD 728
             PELPLNSPITVALP   ++I+RSIS +PGFT  + +KS    P+M        A P R 
Sbjct: 672  -PELPLNSPITVALPDKSTSIERSISTVPGFTVSTYEKS----PTML-----SCAEPQRA 731

Query: 729  SSI-ISNNVVNLNSLRLLK------------SNASSTSLSFLKQDVSI--------SDNN 788
            +S+ +S  +    +L + K            +   + S  F + ++S+        S N+
Sbjct: 732  NSVPVSELLSTTTNLSIQKDIPPIPYGSGWQAREINPSFVFSRHNISLNLPDRVIESRNS 791

Query: 789  MLSSKFT--------SEANISLDNKDDDTVPISGKGEV--SSQLRNSIYISSNALDGNTQ 848
             LS +          S   +  D   ++++ +SG+G V  SSQ+  SI + +       Q
Sbjct: 792  DLSGRSVPVDTPADKSNPIVVADETTNNSLHLSGQGFVRKSSQIGTSIMVEN-------Q 851

Query: 849  ASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRI 908
             +                           SPSD Q+ILVSLS+R VWKGT+C+R+HL RI
Sbjct: 852  DNGXXXXXXXXXXXXXXXXXXXXXXXXXPSPSDHQSILVSLSSRSVWKGTVCERSHLFRI 911

Query: 909  KYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDFFLPG 968
            KYYGSFD+PLGRFLRD LFDQ+YRCRSC MPSEAHV+CYTH QGSLTIS KKL D+ LPG
Sbjct: 912  KYYGSFDKPLGRFLRDHLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDYLLPG 971

Query: 969  EPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCG 1028
            E EGKIWMWHRCL+CPR NGFPPAT RV+MSDAAWGLSFGKFLELSFSN+AAASRVA CG
Sbjct: 972  EKEGKIWMWHRCLRCPRLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCG 1031

Query: 1029 HFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHNRAET 1088
            H LHRDCLRFYGFG  VACF YA I+V SV++PPS++ F + N +WI +E +EV  RAE 
Sbjct: 1032 HSLHRDCLRFYGFGNMVACFRYATIDVHSVYLPPSILSFNYENQDWIQRETDEVIERAEL 1091

Query: 1089 LFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLSGETT 1148
            LF+EV  A+  I +                  I ELE+++  EK +FE +  ++L  E  
Sbjct: 1092 LFSEVLNAISQIAE------------KGFRRRIGELEEVLQKEKAEFEENMQKILHREVN 1151

Query: 1149 VGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGIESVE 1208
             GQP VDI E+ ++H  +LF SY+W  RL  A TL +  +   +  +    ++  +   +
Sbjct: 1152 EGQPLVDILELYRIHRQLLFQSYMWDHRLINASTLHKLENSDDTKRE--ENEKPPLAKSQ 1211

Query: 1209 EFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNS-GQISQLNETEREKDIDLGVL 1268
               E   G                L +  E++L   G+S G    LN  ++E D +  + 
Sbjct: 1212 TLPEMNAGTNS-------------LLTGSEVNLNPDGDSTGDTGSLNNVQKEADTNSDLY 1271

Query: 1269 ED-----DLCLSPVMPQ-----DVEISMRRANSEGEYPIMADLSDTLDAVWKG------- 1328
            ++     ++  S  +P      + ++ +RR  S+G+  +M +LS TLDA W G       
Sbjct: 1272 QEKDDGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQI-VMKNLSATLDAAWIGERQTSVE 1331

Query: 1329 ------VMHPNSATSDSSYSRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTM 1388
                  V  P S  S+SS   T   I E L+     E+ N      + A  +  AL    
Sbjct: 1332 IPTNNKVSLPPSTMSNSS---TFPPISEGLMPIDLPEQQNE----FKVAYPVSPALPSKN 1391

Query: 1389 VDSVETSTSLVSMPSSAYNTKIS---LLNIPKPD-IGNYDPAFISSFRELEKQGYLRLLM 1448
             ++ E S S +S+P   +   I+   LL+  K D  G + P +ISSFRE E QG  RLL+
Sbjct: 1392 YENSEDSVSWLSVPFLNFYRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLL 1451

Query: 1449 HVGSTDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSI 1508
             VG  D VVPVYD+EPTS+IAY+L++PEY  Q S     L          +   +++N  
Sbjct: 1452 PVGLNDIVVPVYDDEPTSMIAYALMSPEYQRQTSAEGESL----------VSYPSELNIP 1511

Query: 1509 KTCDEEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYT 1568
            +  D+   D  ++  S +E+ILS+    S    D L +T+ LH RV +  +  LG VKYT
Sbjct: 1512 RPVDDTIFDPSRSNGSVDESILSISSSRSTSLLDPLSYTKALHARVSYGEDGTLGKVKYT 1571

Query: 1569 ITCYFAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKI 1628
            +TCY+AKRFEALR  C PSEL+Y+RS+SRC++WGAQGGKSNVFFAKTLD+RFIIKQVTK 
Sbjct: 1572 VTCYYAKRFEALRGICLPSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKT 1631

Query: 1629 ELESFFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLF 1688
            ELESF +FAPAYF YLSE+I T  PTCLAK++G+YQV++K  K G+E K+D+L+MENLLF
Sbjct: 1632 ELESFIKFAPAYFKYLSESISTKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLLF 1691

Query: 1689 RHNVTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWND 1748
               V RLYDLKGSSR+RYN D+SG NKVLLDQNLIE+MPTSPIF+G+KAKRLLERAVWND
Sbjct: 1692 GRTVKRLYDLKGSSRARYNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWND 1751

Query: 1749 TSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAP 1764
            T+FLA  DVMDYSLLVG+DEEK ELV+GIIDF+RQYTWDKHLE+WVK +GILGGPKN AP
Sbjct: 1752 TAFLALGDVMDYSLLVGVDEEKNELVLGIIDFLRQYTWDKHLESWVKFTGILGGPKNEAP 1785

BLAST of Carg16457 vs. TAIR10
Match: AT4G33240.1 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases)

HSP 1 Score: 1507.3 bits (3901), Expect = 0.0e+00
Identity = 901/1820 (49.51%), Postives = 1160/1820 (63.74%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            M   ++K    V +VKSW+P +S+  N+SRDFWMPD+SC VCY+CD+QFT  NRRHHCR 
Sbjct: 1    MDSQDHKAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRL 60

Query: 61   CGRVFCSKCTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120
            CGRVFC+KC ANSI +PS +  +S E+ E+IRVCNYC+KQW +      +G        S
Sbjct: 61   CGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNGASIISLHFS 120

Query: 121  LSSSSTSIGSSRS------------------------DYTCHSAISNVNSTPCSNDRQYY 180
             S S+ S+ S+ S                                           R+  
Sbjct: 121  SSPSARSVASTTSXXXXCTIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRRSS 180

Query: 181  DPYSSGVITDEQDNLRNRKSINXXXXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRH 240
            D Y  G + D  DN +    +N   XXXXXXXXXXXXXXXXXXXXXX    Y     + +
Sbjct: 181  DHY--GHVLDSSDN-QVEFFVNSSGXXXXXXXXXXXXXXXXXXXXXXGNDYY----GAIN 240

Query: 241  ISQSDNYYCDVNA-DEGLKLDGEDISKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDF 300
            + + D+ Y    A D G+K++  +IS  G P   D               EN      + 
Sbjct: 241  LDEVDHIYGSHEAHDVGVKIE-PNIS--GFPPDQDLDSLNTETIDKTRQQENGWNDVKEG 300

Query: 301  PTYAMQNTNNEAMDFQNNGILWL-PXXXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDS 360
                 ++   E +DF+++G+LWL P                XXXXX      Y+  S   
Sbjct: 301  SPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDXXXXXXXXXXXYLRPSNSF 360

Query: 361  GECPVKGRSSEEHRKTAKNVVEGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEA 420
             E      S ++     KNVVEGHFRALV+QLLE +NLP+ +E  E+GWLDIIT LSWEA
Sbjct: 361  NEKDF--HSKDKSSGAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSWEA 420

Query: 421  AAVLKPDTSKSGGMDPGGYVKVKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFF 480
            A +LKPDTSKSGGMDPGGYVKVK +  G+RSES++VKG++CKKNVAHRRMT +I KP   
Sbjct: 421  ATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPRLL 480

Query: 481  VLGGALEYQRVTNHLSSLDTLLQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLS 540
            +LGGALEYQR++N LSS DTLLQQE+DHLKMAVAKIDSH+P++LLVEKSVSR AQEYLL+
Sbjct: 481  ILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYLLA 540

Query: 541  KNITLVLNIKRSLLERIACCTGASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAG 600
            K+I+LVLNIKRSLLERI+ CTGA I+P++D + S KLGYCD+F VE FVE H S   Q  
Sbjct: 541  KDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVS-PCQVA 600

Query: 601  KKTTKTLMFFEGCPKPLCCTILLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGA 660
            KK  KTLMFF+GCPKPL CTILL+GA  DELKKVKHV+QY  FAAYHLALET FLADEGA
Sbjct: 601  KKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEGA 660

Query: 661  SVPELPLNSPITVALPHNPSNIDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNR 720
            S+ ELPL +PITVALP  PS ++RSIS IPGFT  S +KS   +   E  +   D   N 
Sbjct: 661  SIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGNF 720

Query: 721  DSS-------IISNNVVNLNSLRLLKSNASSTSLSFLKQDVSISDNNMLSSKFTSEANIS 780
             SS       +  N+ ++  S RLL +    T      + ++  D+ ++ +  + + +  
Sbjct: 721  TSSKTHFQGKLDGNDRID-PSERLLHN--LDTVYCKPPETITSKDDGLVPTLESRQLSFH 780

Query: 781  LDNKDDDTVPISGKGEVSSQLRNSIYISSNALDGNTQASNGSTSQRPESSSTNLVTTKSS 840
            ++         S     + Q+ +  Y + +A+ GN    N +  ++ E          SS
Sbjct: 781  VEEPSVQKDQWSVLSGATEQVTDGGYTNDSAVIGN---QNFNRQEQME----------SS 840

Query: 841  KEDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDEPLGRFLRDRLFDQNY 900
            K DF  S SD Q+ILVSLSTRCVWKG++C+RAHLLRIKYYGSFD+PLGRFLRD LFDQ+ 
Sbjct: 841  KGDFHPSASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQ 900

Query: 901  RCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPP 960
             C SC MP+EAH++CYTH QGSLTIS KKLP+  LPG+ EGKIWMWHRCLKCPR NGFPP
Sbjct: 901  CCPSCTMPAEAHIHCYTHRQGSLTISVKKLPE-LLPGQREGKIWMWHRCLKCPRINGFPP 960

Query: 961  ATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYA 1020
            ATRR++MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRDCLRFYGFGR VACF YA
Sbjct: 961  ATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYA 1020

Query: 1021 AINVQSVHIPPSLIEFYHNNLEWINKEANEVHNRAETLFAEVCKALPLIPKHELGSEQED 1080
            +IN+ +V +PP+ + F + N EW+ KE+ EV  +AE LF EV +AL  I    +G+  + 
Sbjct: 1021 SINIYAVTLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKG 1080

Query: 1081 FGVNELNHYIQELEQIIIMEKEDFEVHFNEVLSGETTVGQPTVDIFEINKLHMHILFLSY 1140
               N++   ++EL  ++   K++++    ++L+     GQPT+DI  INKL   I+F SY
Sbjct: 1081 STPNKIKLSLEELAGLLEQRKKEYKDSLQQMLN-VVKDGQPTIDILLINKLRRLIIFDSY 1140

Query: 1141 IWVQRLSYAVTLRRKN---SPYSSSNDLL-------TLDERGIESVEEFVEFTLGLGQDD 1200
             W + L+ A  + R N   +P +S+  ++        L +  ++S+   V          
Sbjct: 1141 AWDECLAGAANMVRNNYLEAPKNSAPKVMGRNVSLEKLSDEKVKSIPTHVAIC------- 1200

Query: 1201 FFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDLGVLEDDLCLSPVMPQD- 1260
                  NDSL  ++ +E  L +G +    S           D+G      C     P + 
Sbjct: 1201 ------NDSLLQDADYETCLNQGKSFADTSGKFAIPE----DVGSDRPPDCRMEFDPSEG 1260

Query: 1261 -----VEIS--MRRANSEGEYPIMADLSDTLDAVWKGVMHPNSATSDSSYSRTNSAILEP 1320
                 VE S  ++ A++E ++    DLSDTLDA W G       TS++   R  S     
Sbjct: 1261 GKDNFVESSQVVKPAHTESQFQ-ATDLSDTLDAAWIG----EQTTSENGIFRPPSR---- 1320

Query: 1321 LVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNTKIS---LLN 1380
                +  +  +  L+G+E      S L      + +  T+ V +PS ++   ++    LN
Sbjct: 1321 AASTNGTQIPDLRLLGSE------SELNFKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLN 1380

Query: 1381 IPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSLLTPEYN 1440
              K  +    P ++SS+RELE +   RLL+ +G  D V+PVYD+EPTSIIAY+L + EY 
Sbjct: 1381 SRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYK 1440

Query: 1441 AQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSMPRPESL 1500
            AQMS  ++  + +++  S  +  S  + S+ +  + + D  ++L S +E +  +      
Sbjct: 1441 AQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQLLHSS-- 1500

Query: 1501 QFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYVRSISRC 1560
                  L+ +DLH R+ F +E P G VKY++TCY+AK FEALR  CCPSE D++RS+ RC
Sbjct: 1501 ------LYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRC 1560

Query: 1561 RRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGCPTCLAK 1620
            R+WGAQGGKSNVFFAK+LD+RFIIKQVTK ELESF +F PAYF YL+E+I T  PT LAK
Sbjct: 1561 RKWGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAK 1620

Query: 1621 VVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSGKNKVLL 1680
            ++G+YQVSSKH KGG+E K+D+LVMENLLF+ N TRLYDLKGS+R+RYN DTSG N VLL
Sbjct: 1621 ILGIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLL 1680

Query: 1681 DQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGII 1740
            DQNL+E+MPTSPIF+GSKAKRLLERAVWNDTSFLASI VMDYSLLVG+DEE+ ELV+GII
Sbjct: 1681 DQNLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGII 1740

Query: 1741 DFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSS 1767
            DFMRQYTWDKHLETWVK SG+LGGPKN+ PTVISP QYKKRFRKAMT YFLMVPDQW  +
Sbjct: 1741 DFMRQYTWDKHLETWVKTSGLLGGPKNSTPTVISPQQYKKRFRKAMTAYFLMVPDQWSPA 1749

BLAST of Carg16457 vs. TAIR10
Match: AT1G71010.1 (FORMS APLOID AND BINUCLEATE CELLS 1C)

HSP 1 Score: 1061.6 bits (2744), Expect = 5.3e-310
Identity = 607/1433 (42.36%), Postives = 858/1433 (59.87%), Query Frame = 0

Query: 330  SSLDSGECPVKGRSSEEHRKTAKNVVEGHFRALVSQLLEAENLPIGDEPYEDGWLDIITY 389
            SS  S   P K +  E   +  + VV  HFRALV++LL  E L   D+     WLDI+T 
Sbjct: 300  SSSFSSHIPTKEKLGENSNEPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTA 359

Query: 390  LSWEAAAVLKPDTSKSGGMDPGGYVKVKYVAGGKRSESIIVKGIICKKNVAHRRMTREIS 449
            L+W+AA  +KPDT   G MDPG YVK+K VA G ++ESI+++GI+C KN+ H+RM  +  
Sbjct: 360  LAWQAANFVKPDTRAGGSMDPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYK 419

Query: 450  KPCFFVLGGALEYQRVTNHLSSLDTLLQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQ 509
             P   +L G+LEYQRV   L+S +TLLQQE +H+K  +AKI+S  PNVLLVEKS S +AQ
Sbjct: 420  NPRVMLLAGSLEYQRVAGQLASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQ 479

Query: 510  EYLLSKNITLVLNIKRSLLERIACCTGASIIPTVDHVASLKLGYCDVFRVETFVEEHYSG 569
            +YLL K I+LVLN+KRSLL+RIA CTGA + P++D +++ +LG+C++FR E  +E+H +G
Sbjct: 480  QYLLEKEISLVLNVKRSLLDRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAG 539

Query: 570  DGQAGKKTTKTLMFFEGCPKPLCCTILLRGACGDELKKVKHVVQYATFAAYHLALETCFL 629
            + Q+ +K ++TLM+FEGCP+ L CT++LRG+C +ELKKVKHV+QYA FAAYHL+LET FL
Sbjct: 540  N-QSNRKPSRTLMYFEGCPRRLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFL 599

Query: 630  ADEGASVPELPLNSPITVALPHNPSNIDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKD 689
            ADEGAS+P++ L  P  V        ID  IS I      SP ++             + 
Sbjct: 600  ADEGASLPKIRLKQPGMVRTASQRRIIDEGISLI----TQSPTETDSQALLETAAHEDEH 659

Query: 690  AVPNRDSSIISNNVVNLNSLRLL--KSNASSTSLSFLKQDVSISDNNMLSSKFTSEANIS 749
              P  +  +  +   + +  ++    S   +     L  D +   NN+++  ++S     
Sbjct: 660  TAPMPEHEVCESLCEDFDPTQIFPPSSEVETEQSDTLNGDFA---NNLVTRSYSSN---Q 719

Query: 750  LDNKDDDTVPISGK-GEVSSQLRNSIYISSNALDGNTQASNGSTSQRPESSSTNLVTTKS 809
            L++  + T+ +S +  E  +Q       S    +G  +  N   + +      +      
Sbjct: 720  LNDLHEPTLCLSSEIPETPTQ-----QPSGEEDNGRGEEENQLVNPQDLPQHESFYEDDV 779

Query: 810  SKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDEPLGRFLRDRLFDQN 869
            S E F  + S  Q+ILVS S+RCV K ++C+R+ LLRIK+YGSFD+PLGR+L+D LFD+ 
Sbjct: 780  SSEYFSAADS-HQSILVSFSSRCVLKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKT 839

Query: 870  YRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFP 929
              CRSC    +AHV CY+H  G+LTI+ ++LP   LPGE +GKIWMWHRCL+C   +G P
Sbjct: 840  SSCRSCKELVDAHVLCYSHQNGNLTINVRRLPSMKLPGEQDGKIWMWHRCLRCAHVDGVP 899

Query: 930  PATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHY 989
            PATRRV+MSDAAWGLSFGKFLELSFSN+A A+RVA CGH L RDCLRFYGFG  VA F Y
Sbjct: 900  PATRRVVMSDAAWGLSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRY 959

Query: 990  AAINVQSVHIPPSLIEF-YHNNLEWINKEANEVHNRAETLFAEVCKALPLIPKHELGSEQ 1049
            + IN+ +V +PPS++EF  H   EWI  EA E+  +  T++ E+   L  + +     E 
Sbjct: 960  SPINILTVLLPPSMLEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRMEEKSSLLEP 1019

Query: 1050 EDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLSGETTVGQPTVDIFEINKLHMHILFL 1109
            E     +L+  I  L   ++ EK++++     +      + Q ++DI E+N+L   ++  
Sbjct: 1020 EQSEACDLHSRIIGLIDQLVKEKDEYDDALQPIFEENLQI-QGSLDILELNRLRRALMIG 1079

Query: 1110 SYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGIESVEEFVEFTLGLGQDDFFGGCDND 1169
            ++ W  +L    +  +K S + + +D    +                             
Sbjct: 1080 AHAWDHQLYLLNSQLKKASVFKTGDDNAPRNPE--------------------------- 1139

Query: 1170 SLPLESKHEIDLGRGGNSGQISQLNETEREKDIDLGVLEDDLCLSPVMPQDVEISMRRAN 1229
               +    +ID      S +  + + T+ E + D      D       P+++        
Sbjct: 1140 ---MHDPPKIDRRMQEGSDERDEQSHTDSEANGD----NKD-------PENIP------- 1199

Query: 1230 SEGEYPIMADLSDTLDAVWKG----VMHPNSATSDSSYSRTNSAILEPLVGQSDMEKCNA 1289
            S G       LS+ +D+ W G    +    +      +S  NS+ L  L     ++  ++
Sbjct: 1200 SPG-----TSLSERIDSAWLGSFQNLEKAETIAETEGFSAVNSS-LRRLARPIRVQSFDS 1259

Query: 1290 NLVGAETAR--LLHSALVLTMVDSVETSTSLVSMPSSAYN------TKISLLNIPKPD-I 1349
             +   E  +  L  S+L L+ + S   S    +M     +      +++  L + K D I
Sbjct: 1260 AIRFQERIQKGLPPSSLYLSTLRSFHASGEYRNMVRDPVSNVMRTYSQMLPLEVQKLDLI 1319

Query: 1350 GNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEP 1409
                P +ISS  ++   G   L+   G  D VVPVYD++P S+++Y++ + EY   +   
Sbjct: 1320 VGSAPTYISSASQM-ADGARMLIPQRGLNDIVVPVYDDDPASVVSYAINSKEYKEWI--- 1379

Query: 1410 ERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSMPRPESLQFEDLL 1469
                  VN  + L    S+   + +  +  A   +++L    + I       S       
Sbjct: 1380 ------VN--KGLASSSSSSNLNNRESEPSAFSTWRSLSMDVDYIQHAVYGSSQDDRKSP 1439

Query: 1470 LFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYVRSISRCRRWGAQ 1529
              T     R    +    G VK+++TCYFA +F+ LRK CCPSE+D+VRS+SRC+RW AQ
Sbjct: 1440 HLTISFSDRASSSSTATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRCQRWSAQ 1499

Query: 1530 GGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQ 1589
            GGKSNV+FAK+LDERFIIKQV K EL+SF  FAP YF YL E++ +G PTCLAK++G+YQ
Sbjct: 1500 GGKSNVYFAKSLDERFIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAKILGIYQ 1559

Query: 1590 VSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIE 1649
            VS KHPKGG+E K+D++VMENL +   ++R+YDLKGS+RSRYN +TSG +KVLLD NL+E
Sbjct: 1560 VSIKHPKGGKETKMDLMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLLDMNLLE 1619

Query: 1650 SMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQY 1709
            ++ T PIFLGSKAKR LERA+WNDT+FLAS+DVMDYSLLVG DEE++ELV+GIIDFMRQY
Sbjct: 1620 TLRTEPIFLGSKAKRSLERAIWNDTNFLASVDVMDYSLLVGFDEERKELVLGIIDFMRQY 1648

Query: 1710 TWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPS 1746
            TWDKHLETWVK SGILGGPKNA+PT++SP QYK+RFRKAMTTYFL VP+ W S
Sbjct: 1680 TWDKHLETWVKASGILGGPKNASPTIVSPKQYKRRFRKAMTTYFLTVPEPWTS 1648

BLAST of Carg16457 vs. TAIR10
Match: AT1G34260.1 (FORMS APLOID AND BINUCLEATE CELLS 1A)

HSP 1 Score: 664.8 bits (1714), Expect = 1.4e-190
Identity = 492/1434 (34.31%), Postives = 729/1434 (50.84%), Query Frame = 0

Query: 334  SGECPVKGRSSEEHRKTAKNVVEGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWE 393
            S E   K +  EE+R+      +  F+ +VSQL+++    I +  Y   W +I+  L WE
Sbjct: 135  SDESSEKRKVYEENRRVMLEEADSKFKFIVSQLIKSAGFSIEESGY---WFEIVARLCWE 194

Query: 394  AAAVLKPDTSKSGGMDPGGYVKVKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCF 453
            AA++LKP       +DP  Y+KVK +A G   +S + KG++ KK+ A + M  +   P  
Sbjct: 195  AASMLKP-AIDGKSVDPTEYIKVKCIATGSCVDSEVFKGLVFKKHAALKHMATKYEHPRI 254

Query: 454  FVLGGALEYQRVTNHLSSLDTLLQQE---LDHLKMAVAKIDSHHPNVLLVEKSVSRHAQE 513
             ++ G L +    +  SSL ++ Q     L ++K  V  I++  P+V+LVEKSVSR  Q+
Sbjct: 255  MLVEGVLGHP--ISGFSSLQSVNQDNEYLLKYVKPVVDIIEASKPDVMLVEKSVSRDIQK 314

Query: 514  YLLSKNITLVLNIKRSLLERIACCTGASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGD 573
             +L K +TLV ++K   L+RI+ C G+ I+ +VD ++S KL +CD FR+E  VEEH +  
Sbjct: 315  TILDKGVTLVFDMKLHRLQRISRCIGSPIL-SVDSLSSQKLKHCDSFRIEKIVEEH-NAA 374

Query: 574  GQAGKKTTKTLMFFEGCPKPLCCTILLRGACGDELKKVKHVVQYATFAAYHLALETCFLA 633
            G++ KK TKTLMF EGCP  L CTILL+G   + LKKVK VVQY+   AYHL LE  FLA
Sbjct: 375  GESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVKEVVQYSFILAYHLMLEASFLA 434

Query: 634  DEGASVPELPLNSPITVALPHNPSNIDRSISAIPGFTCPSPDKSSEPKPSMEIG-QYGKD 693
            D       +      +             +  I  F+ PSP     P  +++I    G D
Sbjct: 435  DRHTMFSTIFAKEATS------------CVVEIENFS-PSPSPRESPSEAVDIPVSNGFD 494

Query: 694  AVPNRDSSIISNNVVNLNSLRLLKSNASSTSLSFLKQDVSISDNNMLSSKFTSEANISLD 753
                 + +I  N   +   +   +S+            V  +  + LS++ +        
Sbjct: 495  -----EQTIQINGEADGEKVGTWESDGDHVFSHEPYNPVIFTGFSSLSARLSKYLGFV-- 554

Query: 754  NKDDDTVPISGKGEVSSQLRNSIYISSNALDGNTQASNGSTS------QRPESSSTNLVT 813
             ++ ++VP+S   +VS+    S   S    + +T   N          + P +SS++   
Sbjct: 555  -QNPESVPVSVDTDVST---TSNLDSIRESEEDTAEKNEDKQPLLLDPELPVNSSSDDGD 614

Query: 814  TKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDEPLGRFLRDRLF 873
             KS  E+   S  + Q+ILV +S R   +G +CD+ H   IK+Y  FD PL +FLRD +F
Sbjct: 615  NKSQTENDIESTLESQSILVLVSKRNALRGIMCDQRHFSHIKFYKHFDVPLEKFLRD-MF 674

Query: 874  DQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLP-DFFLPGEPEGKIWMWHRCLKCPRT 933
            +Q   C++C    EAH+  Y H    LTI  K++P    L GE +GKIWMW RC KC   
Sbjct: 675  NQRNLCQTCVEFPEAHLYYYAHQNKQLTIQIKRIPVAKGLAGEAKGKIWMWSRCGKCKTK 734

Query: 934  NGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVA 993
            N    +T+RV++S AA  LSFGKFLELSFS     +R + CGH    D L F+G G  VA
Sbjct: 735  NASRKSTKRVLISTAARSLSFGKFLELSFSQQTFLNRSSSCGHSFDSDFLHFFGLGSMVA 794

Query: 994  CFHYAAINVQSVHIPPSLIE-FYHNNLEWINKEANEVHNRAETLFAEVCKALPLIPKHEL 1053
               Y+ +   +V +PP  +E        W+ KE   V  +  +LF +    L  +     
Sbjct: 795  MLSYSQVASYTVSLPPMKLESSILIKAGWLEKEFQTVFTKGISLFEDAAGFLKRLRSQFT 854

Query: 1054 GSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLSGETTVGQPTVDIFEINKLHMH 1113
             S   D         +  +E+++  E+  FE +         T+   +  +  +N++   
Sbjct: 855  NS---DLRYQRARKLLSNIEELLKHERCIFEENIKNSFDKAKTIDDVSHRLLRLNRMRWE 914

Query: 1114 ILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGIESVEEFVEFTLGLGQDDFFGG 1173
            +L  + IW  RL   V   R       S+D   + E+G+++V E        G   +   
Sbjct: 915  LLLQALIWNYRLQSLVLSDR----LLPSSDETKIYEQGLKTVSE-------AGMTRY--- 974

Query: 1174 CDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL---GVLEDDLCLSPVMPQDVE 1233
             +ND+   +S        G N G  + L E    KDI +    V ++D      +P+D E
Sbjct: 975  -ENDNKVSDS--------GSNGGIDTPLVE---HKDIPIAGASVGDNDQMAESYVPEDNE 1034

Query: 1234 ISMRRANSEGEYPIMADLSDTLDAVWKGVMHPNSATSDSSYSRTNSAILEPLVGQS-DME 1293
                 ++S      + +  DT  AV   V   N   +D S          P+ G+S D E
Sbjct: 1035 SQTLCSSSPDTTSPINNHFDTHLAV--NVHSTNGQEADKSI---------PVTGESLDDE 1094

Query: 1294 KCNAN----LVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNTKISLLNIPKPDIG 1353
               +N    L   E   L    L    +  +E            Y  K   +N       
Sbjct: 1095 VSTSNGPHILGWDEWFWLPFEELRSKRIVDIE----------KEYLLKFEYVN------- 1154

Query: 1354 NYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPE 1413
            N+    + +  ++  +   RL + +   D +V  Y++E +S+IA +L      A ++  E
Sbjct: 1155 NFTQENLQTVNQIITEESSRLRISLRDDDFIVSDYEDELSSLIACAL------AHLNN-E 1214

Query: 1414 RVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEET--ILSMPRPESLQFEDL 1473
               +P++      I GS +       D + TD   +  S E T  + ++P PE L     
Sbjct: 1215 ESKKPLSRC----IHGSLQGFLDNNQDSKQTDRDVSRFSSESTNRLETLPPPEVL----- 1274

Query: 1474 LLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYVRSISRCRRWGA 1533
                      V F +   +G  KY+I   +A  F  LRK+CC SELDY+ S+SRC+ W A
Sbjct: 1275 ----------VTFGSVKSVGKPKYSIVSLYADDFRDLRKRCCSSELDYIASLSRCKPWDA 1334

Query: 1534 QGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGCPTCLAKVVGMY 1593
            +GGKS   FAKTLD+RFI+K++ K E ESF  FA  YF Y+ ++   G  TCLAKV+G++
Sbjct: 1335 KGGKSKSVFAKTLDDRFIVKEIKKTEYESFVTFATEYFKYMKDSYDLGNQTCLAKVLGIH 1394

Query: 1594 QVSSKHPK-GGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYN-SDTSGKNKVLLDQN 1653
            QV+ + PK GG+E + D++VMENL F   VTR YDLKG+  +R+  +  +G++ VLLDQN
Sbjct: 1395 QVTVRQPKGGGKEIRHDLMVMENLSFSRKVTRQYDLKGALHARFTATSANGEDDVLLDQN 1450

Query: 1654 LIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFM 1713
             +  M  SP+++   +K+ L+RAV+NDTSFL SI+VMDYSLLVG+D+E  ELV GIID++
Sbjct: 1455 FVNDMNKSPLYVSKTSKQNLQRAVYNDTSFLTSINVMDYSLLVGVDDENHELVCGIIDYL 1450

Query: 1714 RQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQW 1744
            RQYTWDK LETWVK+S ++  PKN  PTVISP  YK RFRK M T+FL VPDQW
Sbjct: 1515 RQYTWDKQLETWVKSSLVV--PKNVQPTVISPIDYKTRFRKFMKTHFLCVPDQW 1450

BLAST of Carg16457 vs. TAIR10
Match: AT5G26360.1 (TCP-1/cpn60 chaperonin family protein)

HSP 1 Score: 79.0 bits (193), Expect = 3.4e-14
Identity = 64/261 (24.52%), Postives = 123/261 (47.13%), Query Frame = 0

Query: 385 DIITYLSWEAAAVLKPDTSKS-GGMDPGGYVKVKYVAGGKRSESIIVKGIICKKN-VAHR 444
           D+I  L+ +A   +  D  +    +D   Y+KV+ V GG+  +S ++KG++  K+ VA  
Sbjct: 168 DLIADLAIDATTTVGVDLGQGLREVDIKKYIKVEKVPGGQFEDSEVLKGVMFNKDVVAPG 227

Query: 445 RMTREISKPCFFVLGGALEYQRVTNHLSS-------LDTLLQQELDHLKMAVAKIDSHHP 504
           +M R+I  P   +L   LEY++  N  ++        + LL+ E ++++    +I    P
Sbjct: 228 KMKRKIVNPRIILLDCPLEYKKGENQTNAELVREEDWEVLLKLEEEYIENICVQILKFKP 287

Query: 505 NVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASIIPTVDHVASLKLGY-C 564
           ++++ EK +S  A  Y     ++ +  ++++   RIA   GA I+   D +    +G   
Sbjct: 288 DLVITEKGLSDLACHYFSKAGVSAIRRLRKTDNNRIAKACGAVIVNRPDELQESDIGTGA 347

Query: 565 DVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGACGDELKKVKHVVQY 624
            +F V+   ++ +S              F   C +P  CT+LLRG   D + +V+  +Q 
Sbjct: 348 GLFEVKKIGDDFFS--------------FIVDCKEPKACTVLLRGPSKDFINEVERNLQD 407

Query: 625 ATFAAYHLALETCFLADEGAS 636
           A   A ++      +   GA+
Sbjct: 408 AMSVARNIIKNPKLVPGGGAT 414

BLAST of Carg16457 vs. Swiss-Prot
Match: sp|Q9LUM0|FAB1B_ARATH (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana OX=3702 GN=FAB1B PE=2 SV=1)

HSP 1 Score: 1562.4 bits (4044), Expect = 0.0e+00
Identity = 913/1847 (49.43%), Postives = 1166/1847 (63.13%), Query Frame = 0

Query: 9    SDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRRCGRVFCSK 68
            S++VGL+KSW+PWRS+P  VSRDFWMPD+SCRVCY+CD QFT INRRHHCR CGRVFC K
Sbjct: 12   SEIVGLIKSWLPWRSEPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCGK 71

Query: 69   CTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGD----KATCSAVSLSSS 128
            CTANSI    +D    RED E+IRVCNYCF+QW       + GD     +  + +S S S
Sbjct: 72   CTANSIPFAPSDLRTPREDWERIRVCNYCFRQW-------EQGDGGPHVSNITELSTSPS 131

Query: 129  STSIGSSRSDYTCHSAISNVNSTP---CSNDRQYY--DPYSSGVITDEQDNLRNRKSI-- 188
             TS+ SS++  T +S+   + S P     N R ++  D    GV + E    +  K    
Sbjct: 132  ETSLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTKQGKETSR 191

Query: 189  -NXXXXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNADEGLKLD 248
             +                             Y++D E+ H  +++ YY  +  + G+ +D
Sbjct: 192  RSSFIATDVEDPSRFALNSIRSDDEYDEYGAYQTDIETSHSPRANEYYGPMEYN-GMGID 251

Query: 249  --------GEDISKK---GSPVMFDNQC----PRDSEESSEDSDENDKGSECD--FPTYA 308
                    GE   +K   GSP++  +QC     R+  E  +   E+D   EC+   P   
Sbjct: 252  DVPCKHLGGETADQKSLSGSPLI--HQCLESLIREGSEQFQKKSEHDGRDECEASSPADI 311

Query: 309  MQNTNNEAMDFQNNGILWLP--XXXXXXXXXXXXXXXXXXXXXAPGEWGYV--PSSLDSG 368
              +   E +DF+NNG+LW+P                       A GEWGY+   +S  SG
Sbjct: 312  SDDQVVEPVDFENNGLLWVPPEPENEEDERESALFDEEDNEGDASGEWGYLRPSTSFGSG 371

Query: 369  ECPVKGRSSEEHRKTAKNVVEGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAA 428
            E   + R++EEH+K  KNVV+GHFRAL++QLL+ EN+ + DE  ++ WL+IIT LSWEAA
Sbjct: 372  EYRGEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAA 431

Query: 429  AVLKPDTSKSGGMDPGGYVKVKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFFV 488
             +LKPD SKSGGMDPGGYVKVK +A G R +S++VKG++CKKNV +RRM+ +I K    +
Sbjct: 432  NLLKPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLI 491

Query: 489  LGGALEYQRVTNHLSSLDTLLQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSK 548
            LGG LEYQRV+N LSS DTLLQQE DHLKMAVAKI +  PN+LLVEKSVSR AQEYLL+K
Sbjct: 492  LGGGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAK 551

Query: 549  NITLVLNIKRSLLERIACCTGASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGK 608
            +I+LVLNIKR LL+RIA CTGA IIP+VDH++S KLGYC+ FRV+ + EEH S  GQ GK
Sbjct: 552  DISLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGS-TGQVGK 611

Query: 609  KTTKTLMFFEGCPKPLCCTILLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGAS 668
            K  KTLM+FE CPKPL  TILLRGA  DELKKVKHVVQY  FAAYHLALET FLADEGAS
Sbjct: 612  KVVKTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGAS 671

Query: 669  VPELPLNSPITVALPHNPSNIDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRD 728
             PELPLNSPITVALP   ++I+RSIS +PGFT  + +KS    P+M        A P R 
Sbjct: 672  -PELPLNSPITVALPDKSTSIERSISTVPGFTVSTYEKS----PTML-----SCAEPQRA 731

Query: 729  SSI-ISNNVVNLNSLRLLK------------SNASSTSLSFLKQDVSI--------SDNN 788
            +S+ +S  +    +L + K            +   + S  F + ++S+        S N+
Sbjct: 732  NSVPVSELLSTTTNLSIQKDIPPIPYGSGWQAREINPSFVFSRHNISLNLPDRVIESRNS 791

Query: 789  MLSSKFT--------SEANISLDNKDDDTVPISGKGEV--SSQLRNSIYISSNALDGNTQ 848
             LS +          S   +  D   ++++ +SG+G V  SSQ+  SI + +       Q
Sbjct: 792  DLSGRSVPVDTPADKSNPIVVADETTNNSLHLSGQGFVRKSSQIGTSIMVEN-------Q 851

Query: 849  ASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRI 908
             +                           SPSD Q+ILVSLS+R VWKGT+C+R+HL RI
Sbjct: 852  DNGXXXXXXXXXXXXXXXXXXXXXXXXXPSPSDHQSILVSLSSRSVWKGTVCERSHLFRI 911

Query: 909  KYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDFFLPG 968
            KYYGSFD+PLGRFLRD LFDQ+YRCRSC MPSEAHV+CYTH QGSLTIS KKL D+ LPG
Sbjct: 912  KYYGSFDKPLGRFLRDHLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDYLLPG 971

Query: 969  EPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCG 1028
            E EGKIWMWHRCL+CPR NGFPPAT RV+MSDAAWGLSFGKFLELSFSN+AAASRVA CG
Sbjct: 972  EKEGKIWMWHRCLRCPRLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCG 1031

Query: 1029 HFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHNRAET 1088
            H LHRDCLRFYGFG  VACF YA I+V SV++PPS++ F + N +WI +E +EV  RAE 
Sbjct: 1032 HSLHRDCLRFYGFGNMVACFRYATIDVHSVYLPPSILSFNYENQDWIQRETDEVIERAEL 1091

Query: 1089 LFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLSGETT 1148
            LF+EV  A+  I +                  I ELE+++  EK +FE +  ++L  E  
Sbjct: 1092 LFSEVLNAISQIAE------------KGFRRRIGELEEVLQKEKAEFEENMQKILHREVN 1151

Query: 1149 VGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGIESVE 1208
             GQP VDI E+ ++H  +LF SY+W  RL  A TL +  +   +  +    ++  +   +
Sbjct: 1152 EGQPLVDILELYRIHRQLLFQSYMWDHRLINASTLHKLENSDDTKRE--ENEKPPLAKSQ 1211

Query: 1209 EFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNS-GQISQLNETEREKDIDLGVL 1268
               E   G                L +  E++L   G+S G    LN  ++E D +  + 
Sbjct: 1212 TLPEMNAGTNS-------------LLTGSEVNLNPDGDSTGDTGSLNNVQKEADTNSDLY 1271

Query: 1269 ED-----DLCLSPVMPQ-----DVEISMRRANSEGEYPIMADLSDTLDAVWKG------- 1328
            ++     ++  S  +P      + ++ +RR  S+G+  +M +LS TLDA W G       
Sbjct: 1272 QEKDDGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQI-VMKNLSATLDAAWIGERQTSVE 1331

Query: 1329 ------VMHPNSATSDSSYSRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTM 1388
                  V  P S  S+SS   T   I E L+     E+ N      + A  +  AL    
Sbjct: 1332 IPTNNKVSLPPSTMSNSS---TFPPISEGLMPIDLPEQQNE----FKVAYPVSPALPSKN 1391

Query: 1389 VDSVETSTSLVSMPSSAYNTKIS---LLNIPKPD-IGNYDPAFISSFRELEKQGYLRLLM 1448
             ++ E S S +S+P   +   I+   LL+  K D  G + P +ISSFRE E QG  RLL+
Sbjct: 1392 YENSEDSVSWLSVPFLNFYRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLL 1451

Query: 1449 HVGSTDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSI 1508
             VG  D VVPVYD+EPTS+IAY+L++PEY  Q S     L          +   +++N  
Sbjct: 1452 PVGLNDIVVPVYDDEPTSMIAYALMSPEYQRQTSAEGESL----------VSYPSELNIP 1511

Query: 1509 KTCDEEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYT 1568
            +  D+   D  ++  S +E+ILS+    S    D L +T+ LH RV +  +  LG VKYT
Sbjct: 1512 RPVDDTIFDPSRSNGSVDESILSISSSRSTSLLDPLSYTKALHARVSYGEDGTLGKVKYT 1571

Query: 1569 ITCYFAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKI 1628
            +TCY+AKRFEALR  C PSEL+Y+RS+SRC++WGAQGGKSNVFFAKTLD+RFIIKQVTK 
Sbjct: 1572 VTCYYAKRFEALRGICLPSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKT 1631

Query: 1629 ELESFFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLF 1688
            ELESF +FAPAYF YLSE+I T  PTCLAK++G+YQV++K  K G+E K+D+L+MENLLF
Sbjct: 1632 ELESFIKFAPAYFKYLSESISTKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLLF 1691

Query: 1689 RHNVTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWND 1748
               V RLYDLKGSSR+RYN D+SG NKVLLDQNLIE+MPTSPIF+G+KAKRLLERAVWND
Sbjct: 1692 GRTVKRLYDLKGSSRARYNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWND 1751

Query: 1749 TSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAP 1764
            T+FLA  DVMDYSLLVG+DEEK ELV+GIIDF+RQYTWDKHLE+WVK +GILGGPKN AP
Sbjct: 1752 TAFLALGDVMDYSLLVGVDEEKNELVLGIIDFLRQYTWDKHLESWVKFTGILGGPKNEAP 1785

BLAST of Carg16457 vs. Swiss-Prot
Match: sp|Q0WUR5|FAB1A_ARATH (1-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana OX=3702 GN=FAB1A PE=2 SV=1)

HSP 1 Score: 1507.3 bits (3901), Expect = 0.0e+00
Identity = 901/1820 (49.51%), Postives = 1160/1820 (63.74%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            M   ++K    V +VKSW+P +S+  N+SRDFWMPD+SC VCY+CD+QFT  NRRHHCR 
Sbjct: 1    MDSQDHKAPGFVDIVKSWIPRKSESSNMSRDFWMPDQSCPVCYECDAQFTVFNRRHHCRL 60

Query: 61   CGRVFCSKCTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120
            CGRVFC+KC ANSI +PS +  +S E+ E+IRVCNYC+KQW +      +G        S
Sbjct: 61   CGRVFCAKCAANSIPSPSDETKDSHEEPERIRVCNYCYKQWEQGIVPPDNGASIISLHFS 120

Query: 121  LSSSSTSIGSSRS------------------------DYTCHSAISNVNSTPCSNDRQYY 180
             S S+ S+ S+ S                                           R+  
Sbjct: 121  SSPSARSVASTTSXXXXCTIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRRSS 180

Query: 181  DPYSSGVITDEQDNLRNRKSINXXXXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRH 240
            D Y  G + D  DN +    +N   XXXXXXXXXXXXXXXXXXXXXX    Y     + +
Sbjct: 181  DHY--GHVLDSSDN-QVEFFVNSSGXXXXXXXXXXXXXXXXXXXXXXGNDYY----GAIN 240

Query: 241  ISQSDNYYCDVNA-DEGLKLDGEDISKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDF 300
            + + D+ Y    A D G+K++  +IS  G P   D               EN      + 
Sbjct: 241  LDEVDHIYGSHEAHDVGVKIE-PNIS--GFPPDQDLDSLNTETIDKTRQQENGWNDVKEG 300

Query: 301  PTYAMQNTNNEAMDFQNNGILWL-PXXXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDS 360
                 ++   E +DF+++G+LWL P                XXXXX      Y+  S   
Sbjct: 301  SPPCEESFEPEVVDFESDGLLWLPPEPENEEDEREAVLSDDXXXXXXXXXXXYLRPSNSF 360

Query: 361  GECPVKGRSSEEHRKTAKNVVEGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEA 420
             E      S ++     KNVVEGHFRALV+QLLE +NLP+ +E  E+GWLDIIT LSWEA
Sbjct: 361  NEKDF--HSKDKSSGAMKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSWEA 420

Query: 421  AAVLKPDTSKSGGMDPGGYVKVKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFF 480
            A +LKPDTSKSGGMDPGGYVKVK +  G+RSES++VKG++CKKNVAHRRMT +I KP   
Sbjct: 421  ATLLKPDTSKSGGMDPGGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPRLL 480

Query: 481  VLGGALEYQRVTNHLSSLDTLLQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLS 540
            +LGGALEYQR++N LSS DTLLQQE+DHLKMAVAKIDSH+P++LLVEKSVSR AQEYLL+
Sbjct: 481  ILGGALEYQRISNQLSSFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYLLA 540

Query: 541  KNITLVLNIKRSLLERIACCTGASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAG 600
            K+I+LVLNIKRSLLERI+ CTGA I+P++D + S KLGYCD+F VE FVE H S   Q  
Sbjct: 541  KDISLVLNIKRSLLERISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVS-PCQVA 600

Query: 601  KKTTKTLMFFEGCPKPLCCTILLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGA 660
            KK  KTLMFF+GCPKPL CTILL+GA  DELKKVKHV+QY  FAAYHLALET FLADEGA
Sbjct: 601  KKMAKTLMFFDGCPKPLGCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEGA 660

Query: 661  SVPELPLNSPITVALPHNPSNIDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNR 720
            S+ ELPL +PITVALP  PS ++RSIS IPGFT  S +KS   +   E  +   D   N 
Sbjct: 661  SIHELPLQTPITVALPDKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGNF 720

Query: 721  DSS-------IISNNVVNLNSLRLLKSNASSTSLSFLKQDVSISDNNMLSSKFTSEANIS 780
             SS       +  N+ ++  S RLL +    T      + ++  D+ ++ +  + + +  
Sbjct: 721  TSSKTHFQGKLDGNDRID-PSERLLHN--LDTVYCKPPETITSKDDGLVPTLESRQLSFH 780

Query: 781  LDNKDDDTVPISGKGEVSSQLRNSIYISSNALDGNTQASNGSTSQRPESSSTNLVTTKSS 840
            ++         S     + Q+ +  Y + +A+ GN    N +  ++ E          SS
Sbjct: 781  VEEPSVQKDQWSVLSGATEQVTDGGYTNDSAVIGN---QNFNRQEQME----------SS 840

Query: 841  KEDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDEPLGRFLRDRLFDQNY 900
            K DF  S SD Q+ILVSLSTRCVWKG++C+RAHLLRIKYYGSFD+PLGRFLRD LFDQ+ 
Sbjct: 841  KGDFHPSASDHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQ 900

Query: 901  RCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPP 960
             C SC MP+EAH++CYTH QGSLTIS KKLP+  LPG+ EGKIWMWHRCLKCPR NGFPP
Sbjct: 901  CCPSCTMPAEAHIHCYTHRQGSLTISVKKLPE-LLPGQREGKIWMWHRCLKCPRINGFPP 960

Query: 961  ATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYA 1020
            ATRR++MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRDCLRFYGFGR VACF YA
Sbjct: 961  ATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYA 1020

Query: 1021 AINVQSVHIPPSLIEFYHNNLEWINKEANEVHNRAETLFAEVCKALPLIPKHELGSEQED 1080
            +IN+ +V +PP+ + F + N EW+ KE+ EV  +AE LF EV +AL  I    +G+  + 
Sbjct: 1021 SINIYAVTLPPAKLYFNYENQEWLQKESKEVIKKAEVLFNEVQEALSQISAKTMGAGSKG 1080

Query: 1081 FGVNELNHYIQELEQIIIMEKEDFEVHFNEVLSGETTVGQPTVDIFEINKLHMHILFLSY 1140
               N++   ++EL  ++   K++++    ++L+     GQPT+DI  INKL   I+F SY
Sbjct: 1081 STPNKIKLSLEELAGLLEQRKKEYKDSLQQMLN-VVKDGQPTIDILLINKLRRLIIFDSY 1140

Query: 1141 IWVQRLSYAVTLRRKN---SPYSSSNDLL-------TLDERGIESVEEFVEFTLGLGQDD 1200
             W + L+ A  + R N   +P +S+  ++        L +  ++S+   V          
Sbjct: 1141 AWDECLAGAANMVRNNYLEAPKNSAPKVMGRNVSLEKLSDEKVKSIPTHVAIC------- 1200

Query: 1201 FFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDLGVLEDDLCLSPVMPQD- 1260
                  NDSL  ++ +E  L +G +    S           D+G      C     P + 
Sbjct: 1201 ------NDSLLQDADYETCLNQGKSFADTSGKFAIPE----DVGSDRPPDCRMEFDPSEG 1260

Query: 1261 -----VEIS--MRRANSEGEYPIMADLSDTLDAVWKGVMHPNSATSDSSYSRTNSAILEP 1320
                 VE S  ++ A++E ++    DLSDTLDA W G       TS++   R  S     
Sbjct: 1261 GKDNFVESSQVVKPAHTESQFQ-ATDLSDTLDAAWIG----EQTTSENGIFRPPSR---- 1320

Query: 1321 LVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNTKIS---LLN 1380
                +  +  +  L+G+E      S L      + +  T+ V +PS ++   ++    LN
Sbjct: 1321 AASTNGTQIPDLRLLGSE------SELNFKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLN 1380

Query: 1381 IPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSLLTPEYN 1440
              K  +    P ++SS+RELE +   RLL+ +G  D V+PVYD+EPTSIIAY+L + EY 
Sbjct: 1381 SRKHIMAEDRPVYVSSYRELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYK 1440

Query: 1441 AQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSMPRPESL 1500
            AQMS  ++  + +++  S  +  S  + S+ +  + + D  ++L S +E +  +      
Sbjct: 1441 AQMSGSDKSRDRLDSGGSFSLFDSVNLLSLNSLSDLSVDMSRSLSSADEQVSQLLHSS-- 1500

Query: 1501 QFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYVRSISRC 1560
                  L+ +DLH R+ F +E P G VKY++TCY+AK FEALR  CCPSE D++RS+ RC
Sbjct: 1501 ------LYLKDLHARISFTDEGPPGKVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRC 1560

Query: 1561 RRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGCPTCLAK 1620
            R+WGAQGGKSNVFFAK+LD+RFIIKQVTK ELESF +F PAYF YL+E+I T  PT LAK
Sbjct: 1561 RKWGAQGGKSNVFFAKSLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAK 1620

Query: 1621 VVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSGKNKVLL 1680
            ++G+YQVSSKH KGG+E K+D+LVMENLLF+ N TRLYDLKGS+R+RYN DTSG N VLL
Sbjct: 1621 ILGIYQVSSKHLKGGKEFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLL 1680

Query: 1681 DQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGII 1740
            DQNL+E+MPTSPIF+GSKAKRLLERAVWNDTSFLASI VMDYSLLVG+DEE+ ELV+GII
Sbjct: 1681 DQNLVEAMPTSPIFVGSKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGII 1740

Query: 1741 DFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPSS 1767
            DFMRQYTWDKHLETWVK SG+LGGPKN+ PTVISP QYKKRFRKAMT YFLMVPDQW  +
Sbjct: 1741 DFMRQYTWDKHLETWVKTSGLLGGPKNSTPTVISPQQYKKRFRKAMTAYFLMVPDQWSPA 1749

BLAST of Carg16457 vs. Swiss-Prot
Match: sp|Q9SSJ8|FAB1C_ARATH (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thaliana OX=3702 GN=FAB1C PE=2 SV=1)

HSP 1 Score: 1061.6 bits (2744), Expect = 9.5e-309
Identity = 607/1433 (42.36%), Postives = 858/1433 (59.87%), Query Frame = 0

Query: 330  SSLDSGECPVKGRSSEEHRKTAKNVVEGHFRALVSQLLEAENLPIGDEPYEDGWLDIITY 389
            SS  S   P K +  E   +  + VV  HFRALV++LL  E L   D+     WLDI+T 
Sbjct: 300  SSSFSSHIPTKEKLGENSNEPLRTVVHDHFRALVAELLRGEELSPSDDGSAGEWLDIVTA 359

Query: 390  LSWEAAAVLKPDTSKSGGMDPGGYVKVKYVAGGKRSESIIVKGIICKKNVAHRRMTREIS 449
            L+W+AA  +KPDT   G MDPG YVK+K VA G ++ESI+++GI+C KN+ H+RM  +  
Sbjct: 360  LAWQAANFVKPDTRAGGSMDPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYK 419

Query: 450  KPCFFVLGGALEYQRVTNHLSSLDTLLQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQ 509
             P   +L G+LEYQRV   L+S +TLLQQE +H+K  +AKI+S  PNVLLVEKS S +AQ
Sbjct: 420  NPRVMLLAGSLEYQRVAGQLASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQ 479

Query: 510  EYLLSKNITLVLNIKRSLLERIACCTGASIIPTVDHVASLKLGYCDVFRVETFVEEHYSG 569
            +YLL K I+LVLN+KRSLL+RIA CTGA + P++D +++ +LG+C++FR E  +E+H +G
Sbjct: 480  QYLLEKEISLVLNVKRSLLDRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAG 539

Query: 570  DGQAGKKTTKTLMFFEGCPKPLCCTILLRGACGDELKKVKHVVQYATFAAYHLALETCFL 629
            + Q+ +K ++TLM+FEGCP+ L CT++LRG+C +ELKKVKHV+QYA FAAYHL+LET FL
Sbjct: 540  N-QSNRKPSRTLMYFEGCPRRLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFL 599

Query: 630  ADEGASVPELPLNSPITVALPHNPSNIDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKD 689
            ADEGAS+P++ L  P  V        ID  IS I      SP ++             + 
Sbjct: 600  ADEGASLPKIRLKQPGMVRTASQRRIIDEGISLI----TQSPTETDSQALLETAAHEDEH 659

Query: 690  AVPNRDSSIISNNVVNLNSLRLL--KSNASSTSLSFLKQDVSISDNNMLSSKFTSEANIS 749
              P  +  +  +   + +  ++    S   +     L  D +   NN+++  ++S     
Sbjct: 660  TAPMPEHEVCESLCEDFDPTQIFPPSSEVETEQSDTLNGDFA---NNLVTRSYSSN---Q 719

Query: 750  LDNKDDDTVPISGK-GEVSSQLRNSIYISSNALDGNTQASNGSTSQRPESSSTNLVTTKS 809
            L++  + T+ +S +  E  +Q       S    +G  +  N   + +      +      
Sbjct: 720  LNDLHEPTLCLSSEIPETPTQ-----QPSGEEDNGRGEEENQLVNPQDLPQHESFYEDDV 779

Query: 810  SKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDEPLGRFLRDRLFDQN 869
            S E F  + S  Q+ILVS S+RCV K ++C+R+ LLRIK+YGSFD+PLGR+L+D LFD+ 
Sbjct: 780  SSEYFSAADS-HQSILVSFSSRCVLKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKT 839

Query: 870  YRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFP 929
              CRSC    +AHV CY+H  G+LTI+ ++LP   LPGE +GKIWMWHRCL+C   +G P
Sbjct: 840  SSCRSCKELVDAHVLCYSHQNGNLTINVRRLPSMKLPGEQDGKIWMWHRCLRCAHVDGVP 899

Query: 930  PATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHY 989
            PATRRV+MSDAAWGLSFGKFLELSFSN+A A+RVA CGH L RDCLRFYGFG  VA F Y
Sbjct: 900  PATRRVVMSDAAWGLSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRY 959

Query: 990  AAINVQSVHIPPSLIEF-YHNNLEWINKEANEVHNRAETLFAEVCKALPLIPKHELGSEQ 1049
            + IN+ +V +PPS++EF  H   EWI  EA E+  +  T++ E+   L  + +     E 
Sbjct: 960  SPINILTVLLPPSMLEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRMEEKSSLLEP 1019

Query: 1050 EDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLSGETTVGQPTVDIFEINKLHMHILFL 1109
            E     +L+  I  L   ++ EK++++     +      + Q ++DI E+N+L   ++  
Sbjct: 1020 EQSEACDLHSRIIGLIDQLVKEKDEYDDALQPIFEENLQI-QGSLDILELNRLRRALMIG 1079

Query: 1110 SYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGIESVEEFVEFTLGLGQDDFFGGCDND 1169
            ++ W  +L    +  +K S + + +D    +                             
Sbjct: 1080 AHAWDHQLYLLNSQLKKASVFKTGDDNAPRNPE--------------------------- 1139

Query: 1170 SLPLESKHEIDLGRGGNSGQISQLNETEREKDIDLGVLEDDLCLSPVMPQDVEISMRRAN 1229
               +    +ID      S +  + + T+ E + D      D       P+++        
Sbjct: 1140 ---MHDPPKIDRRMQEGSDERDEQSHTDSEANGD----NKD-------PENIP------- 1199

Query: 1230 SEGEYPIMADLSDTLDAVWKG----VMHPNSATSDSSYSRTNSAILEPLVGQSDMEKCNA 1289
            S G       LS+ +D+ W G    +    +      +S  NS+ L  L     ++  ++
Sbjct: 1200 SPG-----TSLSERIDSAWLGSFQNLEKAETIAETEGFSAVNSS-LRRLARPIRVQSFDS 1259

Query: 1290 NLVGAETAR--LLHSALVLTMVDSVETSTSLVSMPSSAYN------TKISLLNIPKPD-I 1349
             +   E  +  L  S+L L+ + S   S    +M     +      +++  L + K D I
Sbjct: 1260 AIRFQERIQKGLPPSSLYLSTLRSFHASGEYRNMVRDPVSNVMRTYSQMLPLEVQKLDLI 1319

Query: 1350 GNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEP 1409
                P +ISS  ++   G   L+   G  D VVPVYD++P S+++Y++ + EY   +   
Sbjct: 1320 VGSAPTYISSASQM-ADGARMLIPQRGLNDIVVPVYDDDPASVVSYAINSKEYKEWI--- 1379

Query: 1410 ERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSMPRPESLQFEDLL 1469
                  VN  + L    S+   + +  +  A   +++L    + I       S       
Sbjct: 1380 ------VN--KGLASSSSSSNLNNRESEPSAFSTWRSLSMDVDYIQHAVYGSSQDDRKSP 1439

Query: 1470 LFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYVRSISRCRRWGAQ 1529
              T     R    +    G VK+++TCYFA +F+ LRK CCPSE+D+VRS+SRC+RW AQ
Sbjct: 1440 HLTISFSDRASSSSTATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRSLSRCQRWSAQ 1499

Query: 1530 GGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQ 1589
            GGKSNV+FAK+LDERFIIKQV K EL+SF  FAP YF YL E++ +G PTCLAK++G+YQ
Sbjct: 1500 GGKSNVYFAKSLDERFIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPTCLAKILGIYQ 1559

Query: 1590 VSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIE 1649
            VS KHPKGG+E K+D++VMENL +   ++R+YDLKGS+RSRYN +TSG +KVLLD NL+E
Sbjct: 1560 VSIKHPKGGKETKMDLMVMENLFYNRRISRIYDLKGSARSRYNPNTSGADKVLLDMNLLE 1619

Query: 1650 SMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQY 1709
            ++ T PIFLGSKAKR LERA+WNDT+FLAS+DVMDYSLLVG DEE++ELV+GIIDFMRQY
Sbjct: 1620 TLRTEPIFLGSKAKRSLERAIWNDTNFLASVDVMDYSLLVGFDEERKELVLGIIDFMRQY 1648

Query: 1710 TWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQWPS 1746
            TWDKHLETWVK SGILGGPKNA+PT++SP QYK+RFRKAMTTYFL VP+ W S
Sbjct: 1680 TWDKHLETWVKASGILGGPKNASPTIVSPKQYKRRFRKAMTTYFLTVPEPWTS 1648

BLAST of Carg16457 vs. Swiss-Prot
Match: sp|Q9XID0|FAB1D_ARATH (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thaliana OX=3702 GN=FAB1D PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 2.6e-189
Identity = 492/1434 (34.31%), Postives = 729/1434 (50.84%), Query Frame = 0

Query: 334  SGECPVKGRSSEEHRKTAKNVVEGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWE 393
            S E   K +  EE+R+      +  F+ +VSQL+++    I +  Y   W +I+  L WE
Sbjct: 135  SDESSEKRKVYEENRRVMLEEADSKFKFIVSQLIKSAGFSIEESGY---WFEIVARLCWE 194

Query: 394  AAAVLKPDTSKSGGMDPGGYVKVKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCF 453
            AA++LKP       +DP  Y+KVK +A G   +S + KG++ KK+ A + M  +   P  
Sbjct: 195  AASMLKP-AIDGKSVDPTEYIKVKCIATGSCVDSEVFKGLVFKKHAALKHMATKYEHPRI 254

Query: 454  FVLGGALEYQRVTNHLSSLDTLLQQE---LDHLKMAVAKIDSHHPNVLLVEKSVSRHAQE 513
             ++ G L +    +  SSL ++ Q     L ++K  V  I++  P+V+LVEKSVSR  Q+
Sbjct: 255  MLVEGVLGHP--ISGFSSLQSVNQDNEYLLKYVKPVVDIIEASKPDVMLVEKSVSRDIQK 314

Query: 514  YLLSKNITLVLNIKRSLLERIACCTGASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGD 573
             +L K +TLV ++K   L+RI+ C G+ I+ +VD ++S KL +CD FR+E  VEEH +  
Sbjct: 315  TILDKGVTLVFDMKLHRLQRISRCIGSPIL-SVDSLSSQKLKHCDSFRIEKIVEEH-NAA 374

Query: 574  GQAGKKTTKTLMFFEGCPKPLCCTILLRGACGDELKKVKHVVQYATFAAYHLALETCFLA 633
            G++ KK TKTLMF EGCP  L CTILL+G   + LKKVK VVQY+   AYHL LE  FLA
Sbjct: 375  GESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVKEVVQYSFILAYHLMLEASFLA 434

Query: 634  DEGASVPELPLNSPITVALPHNPSNIDRSISAIPGFTCPSPDKSSEPKPSMEIG-QYGKD 693
            D       +      +             +  I  F+ PSP     P  +++I    G D
Sbjct: 435  DRHTMFSTIFAKEATS------------CVVEIENFS-PSPSPRESPSEAVDIPVSNGFD 494

Query: 694  AVPNRDSSIISNNVVNLNSLRLLKSNASSTSLSFLKQDVSISDNNMLSSKFTSEANISLD 753
                 + +I  N   +   +   +S+            V  +  + LS++ +        
Sbjct: 495  -----EQTIQINGEADGEKVGTWESDGDHVFSHEPYNPVIFTGFSSLSARLSKYLGFV-- 554

Query: 754  NKDDDTVPISGKGEVSSQLRNSIYISSNALDGNTQASNGSTS------QRPESSSTNLVT 813
             ++ ++VP+S   +VS+    S   S    + +T   N          + P +SS++   
Sbjct: 555  -QNPESVPVSVDTDVST---TSNLDSIRESEEDTAEKNEDKQPLLLDPELPVNSSSDDGD 614

Query: 814  TKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDEPLGRFLRDRLF 873
             KS  E+   S  + Q+ILV +S R   +G +CD+ H   IK+Y  FD PL +FLRD +F
Sbjct: 615  NKSQTENDIESTLESQSILVLVSKRNALRGIMCDQRHFSHIKFYKHFDVPLEKFLRD-MF 674

Query: 874  DQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLP-DFFLPGEPEGKIWMWHRCLKCPRT 933
            +Q   C++C    EAH+  Y H    LTI  K++P    L GE +GKIWMW RC KC   
Sbjct: 675  NQRNLCQTCVEFPEAHLYYYAHQNKQLTIQIKRIPVAKGLAGEAKGKIWMWSRCGKCKTK 734

Query: 934  NGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVA 993
            N    +T+RV++S AA  LSFGKFLELSFS     +R + CGH    D L F+G G  VA
Sbjct: 735  NASRKSTKRVLISTAARSLSFGKFLELSFSQQTFLNRSSSCGHSFDSDFLHFFGLGSMVA 794

Query: 994  CFHYAAINVQSVHIPPSLIE-FYHNNLEWINKEANEVHNRAETLFAEVCKALPLIPKHEL 1053
               Y+ +   +V +PP  +E        W+ KE   V  +  +LF +    L  +     
Sbjct: 795  MLSYSQVASYTVSLPPMKLESSILIKAGWLEKEFQTVFTKGISLFEDAAGFLKRLRSQFT 854

Query: 1054 GSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLSGETTVGQPTVDIFEINKLHMH 1113
             S   D         +  +E+++  E+  FE +         T+   +  +  +N++   
Sbjct: 855  NS---DLRYQRARKLLSNIEELLKHERCIFEENIKNSFDKAKTIDDVSHRLLRLNRMRWE 914

Query: 1114 ILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGIESVEEFVEFTLGLGQDDFFGG 1173
            +L  + IW  RL   V   R       S+D   + E+G+++V E        G   +   
Sbjct: 915  LLLQALIWNYRLQSLVLSDR----LLPSSDETKIYEQGLKTVSE-------AGMTRY--- 974

Query: 1174 CDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDL---GVLEDDLCLSPVMPQDVE 1233
             +ND+   +S        G N G  + L E    KDI +    V ++D      +P+D E
Sbjct: 975  -ENDNKVSDS--------GSNGGIDTPLVE---HKDIPIAGASVGDNDQMAESYVPEDNE 1034

Query: 1234 ISMRRANSEGEYPIMADLSDTLDAVWKGVMHPNSATSDSSYSRTNSAILEPLVGQS-DME 1293
                 ++S      + +  DT  AV   V   N   +D S          P+ G+S D E
Sbjct: 1035 SQTLCSSSPDTTSPINNHFDTHLAV--NVHSTNGQEADKSI---------PVTGESLDDE 1094

Query: 1294 KCNAN----LVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNTKISLLNIPKPDIG 1353
               +N    L   E   L    L    +  +E            Y  K   +N       
Sbjct: 1095 VSTSNGPHILGWDEWFWLPFEELRSKRIVDIE----------KEYLLKFEYVN------- 1154

Query: 1354 NYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPE 1413
            N+    + +  ++  +   RL + +   D +V  Y++E +S+IA +L      A ++  E
Sbjct: 1155 NFTQENLQTVNQIITEESSRLRISLRDDDFIVSDYEDELSSLIACAL------AHLNN-E 1214

Query: 1414 RVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEET--ILSMPRPESLQFEDL 1473
               +P++      I GS +       D + TD   +  S E T  + ++P PE L     
Sbjct: 1215 ESKKPLSRC----IHGSLQGFLDNNQDSKQTDRDVSRFSSESTNRLETLPPPEVL----- 1274

Query: 1474 LLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYVRSISRCRRWGA 1533
                      V F +   +G  KY+I   +A  F  LRK+CC SELDY+ S+SRC+ W A
Sbjct: 1275 ----------VTFGSVKSVGKPKYSIVSLYADDFRDLRKRCCSSELDYIASLSRCKPWDA 1334

Query: 1534 QGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGCPTCLAKVVGMY 1593
            +GGKS   FAKTLD+RFI+K++ K E ESF  FA  YF Y+ ++   G  TCLAKV+G++
Sbjct: 1335 KGGKSKSVFAKTLDDRFIVKEIKKTEYESFVTFATEYFKYMKDSYDLGNQTCLAKVLGIH 1394

Query: 1594 QVSSKHPK-GGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYN-SDTSGKNKVLLDQN 1653
            QV+ + PK GG+E + D++VMENL F   VTR YDLKG+  +R+  +  +G++ VLLDQN
Sbjct: 1395 QVTVRQPKGGGKEIRHDLMVMENLSFSRKVTRQYDLKGALHARFTATSANGEDDVLLDQN 1450

Query: 1654 LIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQELVVGIIDFM 1713
             +  M  SP+++   +K+ L+RAV+NDTSFL SI+VMDYSLLVG+D+E  ELV GIID++
Sbjct: 1455 FVNDMNKSPLYVSKTSKQNLQRAVYNDTSFLTSINVMDYSLLVGVDDENHELVCGIIDYL 1450

Query: 1714 RQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVPDQW 1744
            RQYTWDK LETWVK+S ++  PKN  PTVISP  YK RFRK M T+FL VPDQW
Sbjct: 1515 RQYTWDKQLETWVKSSLVV--PKNVQPTVISPIDYKTRFRKFMKTHFLCVPDQW 1450

BLAST of Carg16457 vs. Swiss-Prot
Match: sp|Q9Y2I7|FYV1_HUMAN (1-phosphatidylinositol 3-phosphate 5-kinase OS=Homo sapiens OX=9606 GN=PIKFYVE PE=1 SV=3)

HSP 1 Score: 387.9 bits (995), Expect = 6.1e-106
Identity = 409/1617 (25.29%), Postives = 658/1617 (40.69%), Query Frame = 0

Query: 358  HFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDT-SKSGGMDPGGYVKV 417
            H  AL+ QLL +++L          W DII  L  +    ++PD  ++   MD   +V +
Sbjct: 615  HMMALLQQLLHSDSL-------SSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHI 674

Query: 418  KYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEY-QRVTNHLSSLDTL 477
            K + GGK+ +S++V G +C KN+AH++M+  I  P   +L  ++EY  R     + +D +
Sbjct: 675  KKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPI 734

Query: 478  LQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCT 537
            + QE + LK  V +I    P ++LVEK+VSR AQ+ LL   ITLV+N+K  +LERI+  T
Sbjct: 735  VLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMT 794

Query: 538  GASIIPTVDHVASL-KLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCT 597
               ++ ++D + +   LG C  F ++ F         Q   + TKTLMFFEGCP+ L CT
Sbjct: 795  QGDLVMSMDQLLTKPHLGTCHKFYMQIF---------QLPNEQTKTLMFFEGCPQHLGCT 854

Query: 598  ILLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPI--------- 657
            I LRG    EL +VK ++ +    AYH  LE  FL DE A  P L  N            
Sbjct: 855  IKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGH 914

Query: 658  --------------------TVALPHNPSNI---------------------------DR 717
                                  +LP + S++                           + 
Sbjct: 915  EGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEH 974

Query: 718  SISAIP-----GFTCPSPDK--------------SSEPK----------PSMEIGQYGKD 777
            S +A P      F  P P+               S +P+          P  +I  + +D
Sbjct: 975  STTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAF-RD 1034

Query: 778  AVPNRDSSIISNNVVNLNSLRLLKSNASSTSLS----------FLKQDVSISDNNM---- 837
             + +     ++  V +    R   S A    L             ++   +++  M    
Sbjct: 1035 PLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCST 1094

Query: 838  -------------LSSKFTSEANISLDNKDDDTVPISG-KGEV----------------- 897
                         L+ +F    N        D   + G  G +                 
Sbjct: 1095 RDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTR 1154

Query: 898  ------SSQLRNSIYISSNALDGNTQASNGSTSQRPESSSTNLVTTKSSKEDFP------ 957
                   SQ    +     A  G  Q  N       + +S+       SK +        
Sbjct: 1155 IAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLI 1214

Query: 958  ------------LSPSDQQNILVSLSTRCVWKG---TICDRAHLLRIKYYGSFDEPLGRF 1017
                        L+P + Q + V  S+         + C    ++ +++YG  D  LG F
Sbjct: 1215 LSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIF 1274

Query: 1018 LRDRLFDQNYRCRS--CGMPSEAHVNCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHR 1077
            L    F  +Y+C S  C  P   H+  + H QG + I  K+L D  +PG  +  I  +  
Sbjct: 1275 LERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKEL-DSPVPGY-QHTILTYSW 1334

Query: 1078 CLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAG--CGHFLHRDCLR 1137
            C  C +       T  V +S+ +W +SF K+LEL F  +    R     CGH +H D  +
Sbjct: 1335 CRICKQ------VTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQ 1394

Query: 1138 FYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHNRAETLFAEVCKAL 1197
            ++ + + VA F Y+ I +  V +P   I        +I ++A                  
Sbjct: 1395 YFSYNQMVASFSYSPIRLLEVCVPLPKI--------FIKRQA------------------ 1454

Query: 1198 PLIPKHELGSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHFNEVLSGETTVGQPTVDIF 1257
            PL  K  L  + +DF       Y+   E++  ++ + F     E +  E    Q  ++  
Sbjct: 1455 PL--KVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKM--EDIFAQKEMEEG 1514

Query: 1258 EINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGIESVEEFVEFTLGL 1317
            E              W++++      R  +S   +   L ++ E  I   +   E     
Sbjct: 1515 EFKN-----------WIEKMQ----ARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAW 1574

Query: 1318 GQ--DDFF---GGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDIDLGVLEDDLC 1377
                 D F    G    S+P  S   +   R G   +IS ++ + R              
Sbjct: 1575 NNRLQDLFQQEKGRKRPSVP-PSPGRL---RQGEESKISAMDASPR-------------- 1634

Query: 1378 LSPVMPQDVEISMRRANSEGEYPIMADLSDTLDAVWKGVMHPNSATSDSSYSRTNSAILE 1437
                      IS    N E E   +  LS         +  P      S  S      L+
Sbjct: 1635 ---------NISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELD 1694

Query: 1438 PLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNTKISLLNIP 1497
                  D+   + +L+G+  +++   + +  +  ++        +P ++YN       IP
Sbjct: 1695 TASSSEDV--FDGHLLGSTDSQVKEKSTMKAIFANL--------LPGNSYNP------IP 1754

Query: 1498 KPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSLLTPEYNAQ 1557
             P    +DP       E E+                + V ++EP+SIIA++L   EY   
Sbjct: 1755 FP----FDPDKHYLMYEHER--------------VPIAVCEKEPSSIIAFALSCKEYRNA 1814

Query: 1558 MSEPERVLE--------PVN---------------------------------------- 1617
            + E  +  +        P N                                        
Sbjct: 1815 LEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGM 1874

Query: 1618 ------TARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSMPRPE----SLQFED 1677
                  TA   P + S K  +++  D       QT +   E     P+ E      Q + 
Sbjct: 1875 LSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQ 1934

Query: 1678 LLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCC-PSELDYVRSISRCRRW 1737
            L+    + H  + F +       K+    Y+A  F  +R+     SE D++RS+S    W
Sbjct: 1935 LI----NPHVELQFSDAN----AKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPW 1994

Query: 1738 GAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGCPTCLAKVVG 1744
             A+GGKS   F  T D+RFI+KQ+ ++E++SF  FAP YF Y++ A++   PT LAK++G
Sbjct: 1995 QARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILG 2054

HSP 2 Score: 75.5 bits (184), Expect = 6.7e-12
Identity = 43/138 (31.16%), Postives = 65/138 (47.10%), Query Frame = 0

Query: 8   LSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRRCGRVFCS 67
           LS ++  +K  M  +S   ++ + +WMPD  C+ CYDC  +FT   RRHHCR CG++FCS
Sbjct: 131 LSTVLKRLKEIMEGKSQDSDL-KQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCS 190

Query: 68  KCTANSISAPSTDPTNSREDSEKIRVCNYCFK------------QWGKEPSVLQDGDKAT 127
           +C    I       T        +R C YC K              G++ + L D    +
Sbjct: 191 RCCNQEIPGKFMGYTGD------LRACTYCRKIALSYAHSTDSNSIGEDLNALSD-SACS 250

Query: 128 CSAVSLSSSSTSIGSSRS 134
            S +  S   T +GS ++
Sbjct: 251 VSVLDPSEPRTPVGSRKA 260

BLAST of Carg16457 vs. TrEMBL
Match: tr|A0A1S4DVR6|A0A1S4DVR6_CUCME (LOW QUALITY PROTEIN: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like OS=Cucumis melo OX=3656 GN=LOC103487265 PE=4 SV=1)

HSP 1 Score: 2932.9 bits (7602), Expect = 0.0e+00
Identity = 1525/1800 (84.72%), Postives = 1615/1800 (89.72%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENK+SDLVGLVKSWMPWRSDP NVSRDFWMPDKSCRVCY+CDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKISDLVGLVKSWMPWRSDPANVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120
            CGRVFCSKCTAN I A STDPTN+REDSEKIRVCNYCFKQWGKEPS LQDGDKAT SA+S
Sbjct: 61   CGRVFCSKCTANFIPALSTDPTNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121  LSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINXX 180
            +SSSSTS+GS++S YTCHSAIS++ STPCS  RQYYDPY S  I DE DNLRN K+IN  
Sbjct: 121  VSSSSTSMGSTKSGYTCHSAISHIASTPCSTSRQYYDPYCSAAIADEHDNLRNGKTINRT 180

Query: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNADEGLKLDGEDI 240
            XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSD+YYC VN D GL L+ E+ 
Sbjct: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDDYYCAVNIDGGLNLERENT 240

Query: 241  SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX 300
            S KGSPV FD QCPR +EESSEDSDENDKGSECDFPTYAMQN ++E MDFQNNGILWLP 
Sbjct: 241  STKGSPVNFDTQCPRGNEESSEDSDENDKGSECDFPTYAMQNKDHEPMDFQNNGILWLPP 300

Query: 301  XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR 360
             XXXXXXXXXXXXXXXXX  APGEWG++ SSL SGECP+KG+SSE+++KTAKNVVEGHFR
Sbjct: 301  EXXXXXXXXXXXXXXXXXVCAPGEWGHLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360

Query: 361  ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA 420
            ALVSQLLEAENLPIGD P EDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVK VA
Sbjct: 361  ALVSQLLEAENLPIGDAPNEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420

Query: 421  GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL 480
            GGKRSESIIVKGIICKKNVAHRRMTR ISKP FFVLGGALEYQRVTNHLSS DTLLQQEL
Sbjct: 421  GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481  DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII 540
            DHLKMAVAKID+HHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGA+II
Sbjct: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540

Query: 541  PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVASLKLGYCD FRVETFVEEH SGDGQ GKK+TKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660
             GDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI
Sbjct: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660

Query: 661  SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLR----LLKSNA 720
            SAIPGFTCP P KS EP+P MEIGQ GKDAV +RD SIIS+NV NLNSL     LL+SNA
Sbjct: 661  SAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDLSIISHNVENLNSLEPTLSLLESNA 720

Query: 721  SSTSLSFLKQDVSISDNNMLSSKFTSEANISLDNK---------------DDDTVPISGK 780
            +STS SFLKQD S     MLSSKF SE   SLD+K               D D V  SGK
Sbjct: 721  TSTSFSFLKQDFS----TMLSSKFDSEGKRSLDSKEYSMGMMTIKEEAEEDGDPVSSSGK 780

Query: 781  GEVSSQLRNS-IYISSNALDGNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQN 840
             E SS+  NS I +S N+LD NTQ+ N STSQ PE+S+T+LVTTKSS+EDFPLSPSDQQN
Sbjct: 781  REASSRHANSKILVSRNSLDSNTQSPNDSTSQGPENSNTDLVTTKSSREDFPLSPSDQQN 840

Query: 841  ILVSLSTRCVWKGTICDRAHLLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHV 900
            ILVSLSTRCVWKGTICDRAHLLRIKYYGSFD+PLGRFLRD LFDQNYRC SCGMPSEAHV
Sbjct: 841  ILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHV 900

Query: 901  NCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWG 960
            +CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWG
Sbjct: 901  HCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWG 960

Query: 961  LSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSL 1020
            LSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS+
Sbjct: 961  LSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSM 1020

Query: 1021 IEFYHNNLEWINKEANEVHNRAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQEL 1080
            IEF+ NNLEWINKEANEVHNRA+TLF EVCKALPLI K+E G+EQEDFG +ELN+YIQEL
Sbjct: 1021 IEFHLNNLEWINKEANEVHNRAKTLFDEVCKALPLISKNESGAEQEDFGADELNNYIQEL 1080

Query: 1081 EQIIIMEKEDFEVHFNEVLSGETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLR 1140
            EQII MEKE+FEVHFN+VLSG TTV QPTVDIFEINKLHMHILFLSYIWVQRL+Y  TLR
Sbjct: 1081 EQIINMEKENFEVHFNKVLSGGTTVSQPTVDIFEINKLHMHILFLSYIWVQRLNYVATLR 1140

Query: 1141 RKNSPYSSSNDLLTLDERGIESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRG 1200
            +K+SP  SSND+ TL+E+ + S+EE VE TL  G+D+FFGGCDNDSLP ESK E  LGR 
Sbjct: 1141 KKHSPDGSSNDISTLEEKELNSLEELVELTLASGRDEFFGGCDNDSLPPESKREAGLGRE 1200

Query: 1201 GNSGQISQLNETEREKDIDLGVLEDDLCLSPV--MPQDVEISMRRANSEGEYPIMADLSD 1260
            GNSG IS+LNET REK+IDLG  EDD CLSPV   P DV I +RRANS GEYPIM DLS 
Sbjct: 1201 GNSGNISRLNETNREKNIDLGGQEDDFCLSPVNSEPVDVGIGIRRANSVGEYPIMTDLSG 1260

Query: 1261 TLDAVWKGVMHPNSATSDSSYSRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVL 1320
            TLDAVWKGV+HPNS   + S+SRTNSAILEPL GQSDMEKC AN  G ETA LLHSALVL
Sbjct: 1261 TLDAVWKGVIHPNSLIFEDSFSRTNSAILEPLAGQSDMEKCTAN--GIETAHLLHSALVL 1320

Query: 1321 TMVDSVETSTSLVSMPSSAYNTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHV 1380
            T  DSVET T+  SMP SAYNTKISLLNIPK DI +Y   FISS+RELEKQ +LRLLMHV
Sbjct: 1321 TSDDSVETITNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQSHLRLLMHV 1380

Query: 1381 GSTDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKT 1440
            GS+DTVVPVYDEEPTSIIAYSLL PEY AQMSEP + +E  +TARSLP+IGS KMNSIKT
Sbjct: 1381 GSSDTVVPVYDEEPTSIIAYSLLMPEYIAQMSEPVKGMEASDTARSLPVIGSVKMNSIKT 1440

Query: 1441 CDEEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTIT 1500
            CDEE +DAY+TLRS EETILSMPR ESLQFEDL+  T+DLHTRVCFVNETPLGPVKYTIT
Sbjct: 1441 CDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMTKDLHTRVCFVNETPLGPVKYTIT 1500

Query: 1501 CYFAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIEL 1560
            CYFAKRFEALRKKCCPSELD+VRS+SRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIEL
Sbjct: 1501 CYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIEL 1560

Query: 1561 ESFFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRH 1620
            ESF QF+ AYFIYLS+AI TGCPTCLAKVVGMYQVSSKH KGG+ECK+D+LVMENLLFRH
Sbjct: 1561 ESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVMENLLFRH 1620

Query: 1621 NVTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTS 1680
            NVTRLYDLKGSSRSRYN DTSG NKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTS
Sbjct: 1621 NVTRLYDLKGSSRSRYNPDTSGXNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTS 1680

Query: 1681 FLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTV 1740
            FLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTV
Sbjct: 1681 FLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTV 1740

Query: 1741 ISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1779
            ISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLC+ENSEPE+SWD K Y   SD
Sbjct: 1741 ISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPENSWDCKHYVEHSD 1794

BLAST of Carg16457 vs. TrEMBL
Match: tr|A0A0A0M3A5|A0A0A0M3A5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G662800 PE=4 SV=1)

HSP 1 Score: 2910.9 bits (7545), Expect = 0.0e+00
Identity = 1512/1800 (84.00%), Postives = 1613/1800 (89.61%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MGHPENK+SDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCY+CDSQFTFINRRHHCRR
Sbjct: 1    MGHPENKISDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYECDSQFTFINRRHHCRR 60

Query: 61   CGRVFCSKCTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120
            CGRVFCS+CTAN I APSTD TN+REDSEKIRVCNYCFKQWGKEPS LQDGDKAT SA+S
Sbjct: 61   CGRVFCSRCTANFIPAPSTDATNAREDSEKIRVCNYCFKQWGKEPSSLQDGDKATSSALS 120

Query: 121  LSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITDEQDNLRNRKSINXX 180
            LSSSSTS+GS++S YTCHSAIS++ STPCS  RQYYDPY S  + DE DNLRN K+IN  
Sbjct: 121  LSSSSTSMGSTKSGYTCHSAISHIASTPCSTGRQYYDPYCSAAMADEHDNLRNGKTINRT 180

Query: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNADEGLKLDGEDI 240
            XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSD+YYC VN DEG  L+ E+ 
Sbjct: 181  XXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDDYYCAVNIDEGSNLERENT 240

Query: 241  SKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQNTNNEAMDFQNNGILWLPX 300
            S KGSPV FD Q PR +EESSEDSDENDKGSECDFP+YAMQN N+E MDFQNNGILWLP 
Sbjct: 241  STKGSPVNFDTQSPRGNEESSEDSDENDKGSECDFPSYAMQNKNHEPMDFQNNGILWLPP 300

Query: 301  XXXXXXXXXXXXXXXXXXXXAPGEWGYVPSSLDSGECPVKGRSSEEHRKTAKNVVEGHFR 360
            XXXXXXXXXXXXXXXXXX  APGEWGY+ SSL SGECP+KG+SSE+++KTAKNVVEGHFR
Sbjct: 301  XXXXXXXXXXXXXXXXXXVCAPGEWGYLHSSLSSGECPMKGKSSEDYKKTAKNVVEGHFR 360

Query: 361  ALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKYVA 420
            ALVSQLLEAENLPIGD P EDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVK VA
Sbjct: 361  ALVSQLLEAENLPIGDAPREDGWLDIITYLSWEAAAVLKPDTSKSGGMDPGGYVKVKCVA 420

Query: 421  GGKRSESIIVKGIICKKNVAHRRMTREISKPCFFVLGGALEYQRVTNHLSSLDTLLQQEL 480
            GGKRSESIIVKGIICKKNVAHRRMTR ISKP FFVLGGALEYQRVTNHLSS DTLLQQEL
Sbjct: 421  GGKRSESIIVKGIICKKNVAHRRMTRGISKPRFFVLGGALEYQRVTNHLSSFDTLLQQEL 480

Query: 481  DHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGASII 540
            DHLKMAVAKID+HHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGA+II
Sbjct: 481  DHLKMAVAKIDAHHPNVLLVEKSVSRHAQEYLLSKNITLVLNIKRSLLERIACCTGANII 540

Query: 541  PTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGKKTTKTLMFFEGCPKPLCCTILLRGA 600
            PTVDHVASLKLGYCD FRVETFVEEH SGDGQ GKK+TKTLMFFEGCPKPLCCTILLRGA
Sbjct: 541  PTVDHVASLKLGYCDAFRVETFVEEHDSGDGQGGKKSTKTLMFFEGCPKPLCCTILLRGA 600

Query: 601  CGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNIDRSI 660
             GDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSN+DRSI
Sbjct: 601  SGDELKKVKHVVQYATFAAYHLALETCFLADEGASVPELPLNSPITVALPHNPSNVDRSI 660

Query: 661  SAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPNRDSSIISNNVVNLNSLR----LLKSNA 720
            SAIPGFTCP P KS EP+P MEIGQ GKDAV +RDSSIIS+NVVNLNSL     LL+SNA
Sbjct: 661  SAIPGFTCPLPVKSPEPEPIMEIGQSGKDAVSSRDSSIISHNVVNLNSLEPTSSLLESNA 720

Query: 721  SSTSLSFLKQDVSISDNNMLSSKFTSEANISLDNK---------------DDDTVPISGK 780
            +S S SFLKQD S     M+SS F SE   SL +K               DDD V  SGK
Sbjct: 721  TSPSFSFLKQDFS----TMISSMFDSEGKRSLYSKEYSMGMMNIKEEAGEDDDPVSSSGK 780

Query: 781  GEVSSQLRNS-IYISSNALDGNTQASNGSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQN 840
             E SS+  N+ + ++ N+LD NTQ+ N STSQ PE+ +T+LVTTKSS+EDFPLSPSDQQN
Sbjct: 781  REASSRHANTKVLMNRNSLDANTQSPNNSTSQGPENFNTDLVTTKSSREDFPLSPSDQQN 840

Query: 841  ILVSLSTRCVWKGTICDRAHLLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHV 900
            ILVSLSTRCVWKGTICDRAHLLRIKYYGSFD+PLGRFLRD LFDQNYRC SCGMPSEAHV
Sbjct: 841  ILVSLSTRCVWKGTICDRAHLLRIKYYGSFDKPLGRFLRDNLFDQNYRCHSCGMPSEAHV 900

Query: 901  NCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWG 960
            +CYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWG
Sbjct: 901  HCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWG 960

Query: 961  LSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSL 1020
            LSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPS+
Sbjct: 961  LSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSM 1020

Query: 1021 IEFYHNNLEWINKEANEVHNRAETLFAEVCKALPLIPKHELGSEQEDFGVNELNHYIQEL 1080
            IEF+ NNLEWINKEANEVHNRA+TLF EVCKALPLIPK+E G+EQEDFGV+ELN+YIQEL
Sbjct: 1021 IEFHLNNLEWINKEANEVHNRAKTLFGEVCKALPLIPKNESGAEQEDFGVDELNNYIQEL 1080

Query: 1081 EQIIIMEKEDFEVHFNEVLSGETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLR 1140
            EQII MEKE+FEVHFN++LSG TTV QPTVDIFEINKLHMHILFLSYIWVQRL+Y  TLR
Sbjct: 1081 EQIINMEKENFEVHFNKLLSGGTTVSQPTVDIFEINKLHMHILFLSYIWVQRLNYVATLR 1140

Query: 1141 RKNSPYSSSNDLLTLDERGIESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRG 1200
            +K+SP  SSND+  L E+ + S+EE VE TL  G+D+FFGGCDNDSLP ESK E  LGR 
Sbjct: 1141 KKHSPDGSSNDISILVEKELNSLEELVELTLASGRDEFFGGCDNDSLPAESKREAGLGRE 1200

Query: 1201 GNSGQISQLNETEREKDIDLGVLEDDLCLSPVM--PQDVEISMRRANSEGEYPIMADLSD 1260
            GNSG IS+LN+T+REK+IDLG  EDD CLSPV   P DV I +RRANS GEYPIM  +  
Sbjct: 1201 GNSGNISRLNKTDREKNIDLGGQEDDFCLSPVNLDPVDVGIGIRRANSVGEYPIMT-VPG 1260

Query: 1261 TLDAVWKGVMHPNSATSDSSYSRTNSAILEPLVGQSDMEKCNANLVGAETARLLHSALVL 1320
            TLDAVWKGV+HPNS   D S+SRT SAILEPL GQSDMEKC AN +  ETA LLHSALVL
Sbjct: 1261 TLDAVWKGVIHPNSLIFDDSFSRTTSAILEPLAGQSDMEKCTANRI--ETAHLLHSALVL 1320

Query: 1321 TMVDSVETSTSLVSMPSSAYNTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHV 1380
            T  DSVET  +  SMP SAYNTKISLLNIPK DI +Y   FISS+RELEKQ +LRLLMHV
Sbjct: 1321 TRDDSVETIPNSTSMPPSAYNTKISLLNIPKSDISDYSSVFISSYRELEKQSHLRLLMHV 1380

Query: 1381 GSTDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKT 1440
            GS+DTVVPVYDEEPTSIIAYS+L PEY AQMSEP + +E  +TARSLP+IGS KMNSIKT
Sbjct: 1381 GSSDTVVPVYDEEPTSIIAYSMLMPEYIAQMSEPAKGMEASDTARSLPVIGSVKMNSIKT 1440

Query: 1441 CDEEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTIT 1500
            CDEE +DAY+TLRS EETILSMPR ESLQFEDL+  ++DLHTRVCF+NETPLGPVKYTIT
Sbjct: 1441 CDEEGSDAYKTLRSNEETILSMPRTESLQFEDLVCMSKDLHTRVCFINETPLGPVKYTIT 1500

Query: 1501 CYFAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIEL 1560
            CYFAKRFEALRKKCCPSELD+VRS+SRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIEL
Sbjct: 1501 CYFAKRFEALRKKCCPSELDFVRSLSRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIEL 1560

Query: 1561 ESFFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRH 1620
            ESF QF+ AYFIYLS+AI TGCPTCLAKVVGMYQVSSKH KGG+ECK+D+LVMENLLFRH
Sbjct: 1561 ESFIQFSKAYFIYLSQAICTGCPTCLAKVVGMYQVSSKHLKGGKECKVDVLVMENLLFRH 1620

Query: 1621 NVTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTS 1680
            NVTRLYDLKGSSRSRYN DTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTS
Sbjct: 1621 NVTRLYDLKGSSRSRYNPDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTS 1680

Query: 1681 FLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTV 1740
            FLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTV
Sbjct: 1681 FLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTV 1740

Query: 1741 ISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCEENSEPEHSWDGKPYRYQSD 1779
            ISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLC+ENSEPE+SWD K Y   SD
Sbjct: 1741 ISPHQYKKRFRKAMTTYFLMVPDQWPSSTVSPSESHPDLCDENSEPENSWDCKHYVEHSD 1793

BLAST of Carg16457 vs. TrEMBL
Match: tr|M5W4R3|M5W4R3_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G191000 PE=4 SV=1)

HSP 1 Score: 1884.0 bits (4879), Expect = 0.0e+00
Identity = 1035/1846 (56.07%), Postives = 1288/1846 (69.77%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MG P+NKLS+LV + KSW+P RS+P NVSRDFWMPD+SCRVCYDCDSQFT  NRRHHCR 
Sbjct: 1    MGTPDNKLSELVDIFKSWIPRRSEPPNVSRDFWMPDQSCRVCYDCDSQFTIFNRRHHCRL 60

Query: 61   CGRVFCSKCTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120
            CGRVFC+KCTANS+ APS +    RED E+IRVCNYCF+QW +  + + +G  A    +S
Sbjct: 61   CGRVFCAKCTANSVPAPSDEQRAGREDWERIRVCNYCFRQWEQGIATVDNGPPARSPGLS 120

Query: 121  LSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGV-----------ITDEQD 180
             S S+TS+ S++S  TCH   S + STP S       PY+SG+           +T  QD
Sbjct: 121  PSPSATSLASTKSSCTCHXXXSTIGSTPYSTGPYQRVPYTSGLSPNQSSPQIDSVTATQD 180

Query: 181  NLRNRKSINXXXXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNA 240
            N  +++SI+                            +YR DSE  H S +++YY  VN 
Sbjct: 181  NCTSQRSISSDAAMAESSPNHFGFGMNRSDDEDDDYGVYRLDSEPSHFSHANDYYGAVNI 240

Query: 241  DE--------GLKLDGEDISKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQ 300
            +E         + LDG++ S    P  FD Q    S+E  E+S E++   EC+   Y +Q
Sbjct: 241  EEFDNVYGPHNVHLDGDNTSSL-LPEGFDTQGVEGSQELREESYEHNNCDECETSPYDLQ 300

Query: 301  NTNNEAMDFQNNGILWLP------XXXXXXXXXXXXXXXXXXXXXAPGEWGYVPS--SLD 360
            +TN E +DF+NNG+LWLP      XXXXXXXXXXXXXXXXXXXXXA GEWGY+ S  S  
Sbjct: 301  STNAEPVDFENNGLLWLPXXXXXXXXXXXXXXXXXXXXXXXXXXXATGEWGYLRSSNSFG 360

Query: 361  SGECPVKGRSSEEHRKTAKNVVEGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWE 420
            +GEC  + +S EEHR   KNVVEGHFRALV+QLL+ E+LP+GDE  ++ WLDIIT LSWE
Sbjct: 361  NGECRTREKSIEEHRNAMKNVVEGHFRALVAQLLQVESLPLGDEDNKESWLDIITSLSWE 420

Query: 421  AAAVLKPDTSKSGGMDPGGYVKVKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCF 480
            AA +LKPDTSK GGMDPGGYVKVK +A G+R+ES +VKG++CKKNVAHRRMT +I KP F
Sbjct: 421  AATLLKPDTSKGGGMDPGGYVKVKCIACGRRNESTVVKGVVCKKNVAHRRMTSKIEKPRF 480

Query: 481  FVLGGALEYQRVTNHLSSLDTLLQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLL 540
             +LGGALEYQRV+N LSS DTLLQQE+DHLKMAVAKIDSHHPNVLLVEKSVSR+AQ+YLL
Sbjct: 481  LILGGALEYQRVSNLLSSFDTLLQQEMDHLKMAVAKIDSHHPNVLLVEKSVSRYAQDYLL 540

Query: 541  SKNITLVLNIKRSLLERIACCTGASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQA 600
            +K+I+LVLNIKR LLERIA CTGA I+P++DH+ S KLGYCD+F VE F E H S  GQ 
Sbjct: 541  AKDISLVLNIKRPLLERIARCTGAQIVPSIDHLISPKLGYCDIFHVEKFFEVHGSA-GQG 600

Query: 601  GKKTTKTLMFFEGCPKPLCCTILLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEG 660
            GKK TKTLMFFEGCPKPL  TILLRGA GDELKKVKHVVQY  FAAYHLALET FLADEG
Sbjct: 601  GKKLTKTLMFFEGCPKPLGVTILLRGANGDELKKVKHVVQYGVFAAYHLALETSFLADEG 660

Query: 661  ASVPELPLNSPITVALPHNPSNIDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPN 720
            AS+PELPL S ITVALP  PS+IDRSIS IPGF+ P+  K   P+ S E+ +  K ++ +
Sbjct: 661  ASLPELPLKSVITVALPDKPSSIDRSISTIPGFSVPAAGKPQGPEASSELQKSNKGSISD 720

Query: 721  RDSSIISNNVVNLNSLRLLKSNASSTSLSFL--------------------KQDVSISDN 780
             D     + ++N+     + S+ ++ S +FL                     +D+  S  
Sbjct: 721  SDLCTNIDPILNMEGANSICSSKAACSQAFLGVHSSGSVAPRSPFGSLSHPGEDIRDSFR 780

Query: 781  NMLSSKFTSEANISLDNKDDDTVPISGKGEV--SSQLRNSIYISSNALD---GNTQASN- 840
              L     SE +I +  K+         GE   + +L ++ + +S AL+   GN+ A N 
Sbjct: 781  KKLPGICASENDIDMGCKESFLAKTDKAGEALFNDRLISNSFGASEALEHGGGNSHADNV 840

Query: 841  ----------GSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICD 900
                      G TS +  S + N    +SSKE+FP SPSD Q+ILVSLSTRCVWKGT+C+
Sbjct: 841  DLVANLGEAPGFTSIKNHSDNHN-EEVESSKEEFPPSPSDHQSILVSLSTRCVWKGTVCE 900

Query: 901  RAHLLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKL 960
            R+HL RIKYYG+FD+PLGRFLRD LFDQ+Y CRSCGMPSEAHV+CYTH QGSLTIS KKL
Sbjct: 901  RSHLFRIKYYGNFDKPLGRFLRDHLFDQSYLCRSCGMPSEAHVHCYTHRQGSLTISVKKL 960

Query: 961  PDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAA 1020
            P+  LPGE EGKIWMWHRCL+CPRTNGFPPATRRV+MSDAAWGLSFGKFLELSFSN+AAA
Sbjct: 961  PETLLPGEREGKIWMWHRCLRCPRTNGFPPATRRVVMSDAAWGLSFGKFLELSFSNHAAA 1020

Query: 1021 SRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANE 1080
            +RVA CGH LHRDCLRFYGFGR VACF YA+I+V SV++PPS +EFY++N EWI KEA+E
Sbjct: 1021 NRVATCGHSLHRDCLRFYGFGRMVACFSYASIHVHSVYLPPSKLEFYYDNQEWIQKEADE 1080

Query: 1081 VHNRAETLFAEVCKAL-PLIPKHEL-GSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHF 1140
            + +RAE LF E+  AL  ++ K  L G++       E +H I ELE+++  E+EDFE   
Sbjct: 1081 MGHRAELLFTELRNALNQILGKRPLAGTQDGGKKAPESSHQIAELEEMLQKEREDFEESL 1140

Query: 1141 NEVLSGETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTL 1200
             +V+  E   G P +DI EINKL   +LF SY+W QRL +A +L  K      S+ L  L
Sbjct: 1141 RKVMHREVKFGHPAIDILEINKLRRQLLFHSYVWDQRLIHAASLSNKGFQEGLSSSLPKL 1200

Query: 1201 DERGIESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLN--ETE 1260
             E+ + S+E+  E  +          C  DS  LE+K +I++ +GG+ G  S     + +
Sbjct: 1201 KEKPLSSMEKLAETNINSKPGKGVSIC--DSSLLETKPDINVNQGGDVGYFSPPGGVQNK 1260

Query: 1261 REKDIDLGVLEDDLCLSPVMPQDVE--------ISMRRANSEG-EYPIMADLSDTLDAVW 1320
             E  +DL    +    +P  P  ++         S+RRA SEG E P +A+LSDTLDA W
Sbjct: 1261 TEMGLDLNHSNEADLSTPSFPNVIDKSDPLESGKSVRRALSEGDECPTVANLSDTLDAAW 1320

Query: 1321 KGVMHPNSA-TSDSSYSRTNSAI------LEPLVGQSDMEKCNANLVGAETARLLHSALV 1380
             G  HP S    D+ YS  +S +      +  +   SD++    + VG +    L S L 
Sbjct: 1321 TGESHPTSTIPKDNGYSIPDSTLVNSPTAIRKVASNSDLQNYTIDQVGVQVTHSLSSPLH 1380

Query: 1381 LTMVDSVETSTSLVSMPSSAYNTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMH 1440
            L                   ++  IS LN  K  IG  +P ++  FRELE+Q   RLL+ 
Sbjct: 1381 L-----------------KGFDKNIS-LNAQKLFIGEGNPVYVPLFRELERQSGARLLLP 1440

Query: 1441 VGSTDTVVPVYDEEPTSIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIK 1500
            +G  DTV+PV+D+EPTSIIAY+L++P+Y+ Q+SE ER  + ++++ SLP+  SA + S+ 
Sbjct: 1441 IGVNDTVIPVFDDEPTSIIAYALVSPDYHLQISESERPKDALDSSVSLPLFDSANLLSLT 1500

Query: 1501 TCDEEATDAYQTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTI 1560
            + DE  ++ Y+ L S +E+++S  R  S Q  D LL ++DLH RV F ++ PLG VKYT+
Sbjct: 1501 SFDEAVSETYRNLGSSDESLISTSRSRSSQALDSLL-SKDLHARVSFTDDGPLGKVKYTV 1560

Query: 1561 TCYFAKRFEALRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIE 1620
            TCY+A RFEALR+ CCPSE+D+VRS+SRC++WGAQGGKSNVFFAKTLD+RFIIKQVTK E
Sbjct: 1561 TCYYATRFEALRRTCCPSEIDFVRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTE 1620

Query: 1621 LESFFQFAPAYFIYLSEAIRTGCPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFR 1680
            LESF +FAP+YF YLSE+I T  PTCLAK++G+YQVSSKH KGG+E K+D+LVMENLLFR
Sbjct: 1621 LESFIKFAPSYFKYLSESISTRSPTCLAKILGIYQVSSKHGKGGKESKMDVLVMENLLFR 1680

Query: 1681 HNVTRLYDLKGSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDT 1740
             NVTRLYDLKGSSRSRYN DTSG NKVLLDQNLIE+MPTSPIF+G+KAKRLLERAVWNDT
Sbjct: 1681 RNVTRLYDLKGSSRSRYNPDTSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDT 1740

Query: 1741 SFLASIDVMDYSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPT 1764
            +FLASIDVMDYSLLVG+DEEK ELV+GIIDF+RQYTWDKHLETWVK SG+LGGPKN +PT
Sbjct: 1741 AFLASIDVMDYSLLVGVDEEKDELVLGIIDFVRQYTWDKHLETWVKTSGLLGGPKNTSPT 1800

BLAST of Carg16457 vs. TrEMBL
Match: tr|A0A2C9UB18|A0A2C9UB18_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_16G089800 PE=4 SV=1)

HSP 1 Score: 1874.8 bits (4855), Expect = 0.0e+00
Identity = 1020/1820 (56.04%), Postives = 1251/1820 (68.74%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MG+ +NK SD V +VKSW+P RS+P NVSRDFWMPD+SCRVCY+CDSQFT  NRRHHCR 
Sbjct: 1    MGNSDNKRSDFVDIVKSWIPRRSEPANVSRDFWMPDQSCRVCYECDSQFTIFNRRHHCRL 60

Query: 61   CGRVFCSKCTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120
            CGRVFC+KCTA+SI APS DP    ED E+IRVCNYCFKQW +  + + +G       +S
Sbjct: 61   CGRVFCAKCTASSIPAPSEDPRTGSEDWERIRVCNYCFKQWQQGTTAVDNGTNVGSPGLS 120

Query: 121  LSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGVITD----------EQDN 180
             S S+TS+ SS+S  TC+S+ S V STPCS+      PYSSG+             EQ+N
Sbjct: 121  PSPSATSLVSSKSCCTCNSS-STVGSTPCSSGAYQSVPYSSGLSPQQSAPMDRTVVEQEN 180

Query: 181  LRNRKSINXXXXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNAD 240
            L   +S +                            +Y S S +RH S +D YY     D
Sbjct: 181  LTCGRSSDAPAAAFCSSANQFGYCMSRSDDEDDEYGVYHSGSGTRHFSHADGYYGPGTVD 240

Query: 241  E--------GLKLDGE-DISKKGSPVM--FDNQCPRDSEESSEDSDENDKGSECDFPTYA 300
            +        G+  + + D S    P+   FD Q     +   E++  +D  +EC+ P+Y 
Sbjct: 241  DIDHMYEPCGIMHEDQIDRSISCPPLSENFDTQVVDKIKNDGEEAYRHD-CNECESPSYG 300

Query: 301  MQNTNNEAMDFQNNGILWLP--XXXXXXXXXXXXXXXXXXXXXAPGEWGYV--PSSLDSG 360
            +   + E +DF+NNG+LWLP     XXX                 GEWGY+   +S  SG
Sbjct: 301  VNGADAEPVDFENNGLLWLPPEPEDXXXEREAVLFDEDDGDEATTGEWGYLRPSNSFGSG 360

Query: 361  ECPVKGRSSEEHRKTAKNVVEGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWEAA 420
            E   K +SSEEHRK  KNVVEGHFRALV+QLL+ ENL +GDE   + WL+IIT LSWEAA
Sbjct: 361  EYRSKDKSSEEHRKAMKNVVEGHFRALVAQLLQVENLSVGDEDDNESWLEIITSLSWEAA 420

Query: 421  AVLKPDTSKSGGMDPGGYVKVKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCFFV 480
             +LKPDTS SGGMDPGGYVKVK +A G+RSES++VKG++CKKNVAHRRM  +I KP F +
Sbjct: 421  TLLKPDTSMSGGMDPGGYVKVKCIASGRRSESMVVKGVVCKKNVAHRRMMSKIDKPRFLI 480

Query: 481  LGGALEYQRVTNHLSSLDTLLQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLLSK 540
            LGGALEYQRV+NHLSS+DTLLQQE+DHLKMAVAKID+HHP+VLLVEKSVSR+AQ+YLL+K
Sbjct: 481  LGGALEYQRVSNHLSSVDTLLQQEMDHLKMAVAKIDAHHPSVLLVEKSVSRYAQDYLLAK 540

Query: 541  NITLVLNIKRSLLERIACCTGASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQAGK 600
            +I+LVLNIKR LLERIA CTGA I+P++DH+ S KLGYCD+F VE F+EEH S  GQ GK
Sbjct: 541  DISLVLNIKRPLLERIARCTGAHIVPSIDHLNSQKLGYCDLFHVEKFLEEHGSA-GQGGK 600

Query: 601  KTTKTLMFFEGCPKPLCCTILLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEGAS 660
            K TKTLMFFEGCPKPL  TILL+GA GD+LKKVKHVVQY  FAAYHLALET FLADEGAS
Sbjct: 601  KLTKTLMFFEGCPKPLGFTILLKGAHGDDLKKVKHVVQYGVFAAYHLALETSFLADEGAS 660

Query: 661  VPELPLNSPITVALPHNPSNIDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPN-- 720
            +PELPL SP+TVALP  P +IDRSIS IPGFT P   K S  +P  E+ +   D + +  
Sbjct: 661  LPELPLKSPLTVALPDKPLSIDRSISTIPGFTVPGTGKLSSCQPPSEVQKSNNDVISDVP 720

Query: 721  -------------RDSSIISNNVVNLNSLRLLKSNA-----SSTSLSFLKQDVSISDNNM 780
                          DS+  SN+  +    R L SN+     S TSLS L Q+ S   +N 
Sbjct: 721  SPTNFELVCKSGYADSTSPSNS--SQIEYRNLGSNSTEHAGSWTSLSPLGQNTSGPCHNK 780

Query: 781  LSSKFTSEANISLDNKDDDTVPISGKGEVSSQLRNSIYISSNALDGNTQASNGSTSQRPE 840
            L S      +   + ++        +G+ ++    S Y S     G+  A     + + +
Sbjct: 781  LFSDHVPGKDYMTEAEE------LFQGKKANSDSPSYYTSLEQGTGSGHADENVLAVKQD 840

Query: 841  SSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICDRAHLLRIKYYGSFDEPLG 900
             +  N     SSKEDFP SPSD Q+ILVSLSTRC+WKGT+C+RAHL RIKYYGSFD+PLG
Sbjct: 841  GN--NHEEIGSSKEDFPPSPSDHQSILVSLSTRCIWKGTVCERAHLFRIKYYGSFDKPLG 900

Query: 901  RFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKLPDFFLPGEPEGKIWMWHR 960
            RFLRD LFDQNYRCRSC MPSEAHV CYTH QGSLTIS K+LP+F LPGE EGKIWMWHR
Sbjct: 901  RFLRDHLFDQNYRCRSCEMPSEAHVYCYTHRQGSLTISVKRLPEFLLPGEREGKIWMWHR 960

Query: 961  CLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAASRVAGCGHFLHRDCLRFY 1020
            CL+CPR+NGFPPATRR++MSDAAWGLSFGKFLELSFSN+AAASRVA CGH LHRDCLRFY
Sbjct: 961  CLRCPRSNGFPPATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVASCGHSLHRDCLRFY 1020

Query: 1021 GFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANEVHNRAETLFAEVCKALPL 1080
            GFGR VACF YA+I+V SVH+PPS +EF ++N EWI KEANEV  RAE LF EV  AL  
Sbjct: 1021 GFGRMVACFRYASIDVHSVHLPPSKLEFNYDNQEWIQKEANEVRQRAELLFTEVQNALHR 1080

Query: 1081 IPKHEL--GSEQEDFGVNELNHY-IQELEQIIIMEKEDFEVHFNEVLSGETTVGQPTVDI 1140
            I +  L  GS+  D   +EL+ + + EL+ I+  EK +FE      LS E  VG P +D+
Sbjct: 1081 ISEKILGEGSQNGDIKSSELSRFHVAELKSILQKEKAEFEESLLNTLSKEVKVGHPLIDL 1140

Query: 1141 FEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTLDERGIESVEEFVEFTLG 1200
             E+NKL   ILF SY+W QRL YAV+L   N   S  N +  L  + I SVE  VE    
Sbjct: 1141 LEVNKLRRQILFHSYVWDQRLIYAVSLSNTNHQESMRNFIPKLGGKRINSVESLVEMDTS 1200

Query: 1201 LGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETEREKDI--DLGVLEDDLCLS 1260
                  F  C  DS+ + +K E++L +GGN G   Q        +I  DL + + + CLS
Sbjct: 1201 PKPGKGFSNC--DSVIVHTKPELNLNQGGNDGSSRQSVRVHEGGNIRLDLKLKDSEHCLS 1260

Query: 1261 -------PVMPQDVEISMRRANSEGEYPIMADLSDTLDAVWKGVMHPNS-ATSDSSYSRT 1320
                    + P +    MRR +SEGE+PI+ +LSDTLDA W G  H  S    ++  S  
Sbjct: 1261 SCENSNEKIDPSESGNVMRRVHSEGEFPIVDNLSDTLDAAWTGKNHLESMILKENGVSLP 1320

Query: 1321 NSAILEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETSTSLVSMPSSAYNT-- 1380
            +S+ +  +V   ++E+C  +  G +    L SAL   +  +VE S+ L +   + Y++  
Sbjct: 1321 DSSPVHSVVPNVELERCIVDKGGIDVVHSLDSALGAKVPQNVENSSGLGTPFPNLYSSFK 1380

Query: 1381 KISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPVYDEEPTSIIAYSL 1440
            + S LN  K  I  Y+P ++S FR+LE+    RLL+ VG  DT++PV+D+EPTSIIAY+L
Sbjct: 1381 RTSSLNAQKLGINEYNPVYVSLFRDLERPNNARLLLPVGVNDTIIPVFDDEPTSIIAYAL 1440

Query: 1441 LTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAYQTLRSKEETILSM 1500
            ++ +Y+ QMSE E+  +   T  SLP+  S  + S  + DE A+D Y+++ S EE ILS+
Sbjct: 1441 VSSDYHLQMSELEKPKDAGETTISLPLFDSVNLLSFNSFDESASDIYRSVGSIEENILSI 1500

Query: 1501 PRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEALRKKCCPSELDYV 1560
            P     Q  D L +T+DLH RV F +++  G VKY +TCY AKRFEALR+ CCPSELD++
Sbjct: 1501 PGSRGTQILDPLSYTKDLHARVSFTDDSVQGKVKYMVTCYCAKRFEALRRICCPSELDFI 1560

Query: 1561 RSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPAYFIYLSEAIRTGC 1620
            RS+SRC++WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESF +F PAYF YLS++I  G 
Sbjct: 1561 RSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFGPAYFKYLSDSIGNGS 1620

Query: 1621 PTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLKGSSRSRYNSDTSG 1680
            PTCLAK++G+YQVSSKH KGG+E K+D+LVMENLLF+ N+ RLYDLKGSSRSRYNSDTSG
Sbjct: 1621 PTCLAKILGIYQVSSKHLKGGKETKMDLLVMENLLFKRNIVRLYDLKGSSRSRYNSDTSG 1680

Query: 1681 KNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMDYSLLVGLDEEKQE 1740
             NKVLLDQNLIESMPTSPIF+G+KAKRLLERAVWNDTSFLASIDVMDYSLLVG+D E+ E
Sbjct: 1681 SNKVLLDQNLIESMPTSPIFVGNKAKRLLERAVWNDTSFLASIDVMDYSLLVGVDTERHE 1740

Query: 1741 LVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKRFRKAMTTYFLMVP 1761
            LV+GIIDFMRQYTWDKHLETWVK SGILGG KN  PTVISP QYKKRFRKAMT YFLMVP
Sbjct: 1741 LVIGIIDFMRQYTWDKHLETWVKASGILGGSKNTTPTVISPQQYKKRFRKAMTAYFLMVP 1800

BLAST of Carg16457 vs. TrEMBL
Match: tr|A0A251NDN9|A0A251NDN9_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G191000 PE=4 SV=1)

HSP 1 Score: 1859.0 bits (4814), Expect = 0.0e+00
Identity = 1026/1836 (55.88%), Postives = 1267/1836 (69.01%), Query Frame = 0

Query: 1    MGHPENKLSDLVGLVKSWMPWRSDPENVSRDFWMPDKSCRVCYDCDSQFTFINRRHHCRR 60
            MG P+NKLS+LV + KSW+P RS+P NVSRDFWMPD+SCRVCYDCDSQFT  NRRHHCR 
Sbjct: 1    MGTPDNKLSELVDIFKSWIPRRSEPPNVSRDFWMPDQSCRVCYDCDSQFTIFNRRHHCRL 60

Query: 61   CGRVFCSKCTANSISAPSTDPTNSREDSEKIRVCNYCFKQWGKEPSVLQDGDKATCSAVS 120
            CGRVFC+KCTANS+ APS +    RED E+IRVCNYCF+QW +  + + +G  A    +S
Sbjct: 61   CGRVFCAKCTANSVPAPSDEQRAGREDWERIRVCNYCFRQWEQGIATVDNGPPARSPGLS 120

Query: 121  LSSSSTSIGSSRSDYTCHSAISNVNSTPCSNDRQYYDPYSSGV-----------ITDEQD 180
             S S+TS+ S++S  TCH   S + STP S       PY+SG+           +T  QD
Sbjct: 121  PSPSATSLASTKSSCTCHXXXSTIGSTPYSTGPYQRVPYTSGLSPNQSSPQIDSVTATQD 180

Query: 181  NLRNRKSINXXXXXXXXXXXXXXXXXXXXXXXXXXXXMYRSDSESRHISQSDNYYCDVNA 240
            N  +++SI+                            +YR DSE  H S +++YY  VN 
Sbjct: 181  NCTSQRSISSDAAMAESSPNHFGFGMNRSDDEDDDYGVYRLDSEPSHFSHANDYYGAVNI 240

Query: 241  DE--------GLKLDGEDISKKGSPVMFDNQCPRDSEESSEDSDENDKGSECDFPTYAMQ 300
            +E         + LDG++ S    P  FD Q    S+E  E+S E++   EC+   Y +Q
Sbjct: 241  EEFDNVYGPHNVHLDGDNTSSL-LPEGFDTQGVEGSQELREESYEHNNCDECETSPYDLQ 300

Query: 301  NTNNEAMDFQNNGILWLP------XXXXXXXXXXXXXXXXXXXXXAPGEWGYVPS--SLD 360
            +TN E +DF+NNG+LWLP      XXXXXXXXXXXXXXXXXXXXXA GEWGY+ S  S  
Sbjct: 301  STNAEPVDFENNGLLWLPXXXXXXXXXXXXXXXXXXXXXXXXXXXATGEWGYLRSSNSFG 360

Query: 361  SGECPVKGRSSEEHRKTAKNVVEGHFRALVSQLLEAENLPIGDEPYEDGWLDIITYLSWE 420
            +GEC  + +S EEHR   KNVVEGHFRALV+QLL+ E+LP+GDE  ++ WLDIIT LSWE
Sbjct: 361  NGECRTREKSIEEHRNAMKNVVEGHFRALVAQLLQVESLPLGDEDNKESWLDIITSLSWE 420

Query: 421  AAAVLKPDTSKSGGMDPGGYVKVKYVAGGKRSESIIVKGIICKKNVAHRRMTREISKPCF 480
            AA +LKPDTSK GGMDPGGYVKVK +A G+R+ES +VKG++CKKNVAHRRMT +I KP F
Sbjct: 421  AATLLKPDTSKGGGMDPGGYVKVKCIACGRRNESTVVKGVVCKKNVAHRRMTSKIEKPRF 480

Query: 481  FVLGGALEYQRVTNHLSSLDTLLQQELDHLKMAVAKIDSHHPNVLLVEKSVSRHAQEYLL 540
             +LGGALEYQRV+N LSS DTLLQQE+DHLKMAVAKIDSHHPNVLLVEKSVSR+AQ+YLL
Sbjct: 481  LILGGALEYQRVSNLLSSFDTLLQQEMDHLKMAVAKIDSHHPNVLLVEKSVSRYAQDYLL 540

Query: 541  SKNITLVLNIKRSLLERIACCTGASIIPTVDHVASLKLGYCDVFRVETFVEEHYSGDGQA 600
            +K+I+LVLNIKR LLERIA CTGA I+P++DH+ S KLGYCD+F VE F E H S  GQ 
Sbjct: 541  AKDISLVLNIKRPLLERIARCTGAQIVPSIDHLISPKLGYCDIFHVEKFFEVHGSA-GQG 600

Query: 601  GKKTTKTLMFFEGCPKPLCCTILLRGACGDELKKVKHVVQYATFAAYHLALETCFLADEG 660
            GKK TKTLMFFEGCPKPL  TILLRGA GDELKKVKHVVQY  FAAYHLALET FLADEG
Sbjct: 601  GKKLTKTLMFFEGCPKPLGVTILLRGANGDELKKVKHVVQYGVFAAYHLALETSFLADEG 660

Query: 661  ASVPELPLNSPITVALPHNPSNIDRSISAIPGFTCPSPDKSSEPKPSMEIGQYGKDAVPN 720
            AS+PELPL S ITVALP  PS+IDRSIS IPGF+ P+  K   P+ S E+ +  K ++ +
Sbjct: 661  ASLPELPLKSVITVALPDKPSSIDRSISTIPGFSVPAAGKPQGPEASSELQKSNKGSISD 720

Query: 721  RDSSIISNNVVNLNSLRLLKSNASSTSLSFL--------------------KQDVSISDN 780
             D     + ++N+     + S+ ++ S +FL                     +D+  S  
Sbjct: 721  SDLCTNIDPILNMEGANSICSSKAACSQAFLGVHSSGSVAPRSPFGSLSHPGEDIRDSFR 780

Query: 781  NMLSSKFTSEANISLDNKDDDTVPISGKGEV--SSQLRNSIYISSNALD---GNTQASN- 840
              L     SE +I +  K+         GE   + +L ++ + +S AL+   GN+ A N 
Sbjct: 781  KKLPGICASENDIDMGCKESFLAKTDKAGEALFNDRLISNSFGASEALEHGGGNSHADNV 840

Query: 841  ----------GSTSQRPESSSTNLVTTKSSKEDFPLSPSDQQNILVSLSTRCVWKGTICD 900
                      G TS +  S + N    +SSKE+FP SPSD Q+ILVSLSTRCVWKGT+C+
Sbjct: 841  DLVANLGEAPGFTSIKNHSDNHN-EEVESSKEEFPPSPSDHQSILVSLSTRCVWKGTVCE 900

Query: 901  RAHLLRIKYYGSFDEPLGRFLRDRLFDQNYRCRSCGMPSEAHVNCYTHPQGSLTISAKKL 960
            R+HL RIKYYG+FD+PLGRFLRD LFDQ+Y CRSCGMPSEAHV+CYTH QGSLTIS KKL
Sbjct: 901  RSHLFRIKYYGNFDKPLGRFLRDHLFDQSYLCRSCGMPSEAHVHCYTHRQGSLTISVKKL 960

Query: 961  PDFFLPGEPEGKIWMWHRCLKCPRTNGFPPATRRVIMSDAAWGLSFGKFLELSFSNNAAA 1020
            P+  LPGE EGKIWMWHRCL+CPRTNGFPPATRRV+MSDAAWGLSFGKFLELSFSN+AAA
Sbjct: 961  PETLLPGEREGKIWMWHRCLRCPRTNGFPPATRRVVMSDAAWGLSFGKFLELSFSNHAAA 1020

Query: 1021 SRVAGCGHFLHRDCLRFYGFGRTVACFHYAAINVQSVHIPPSLIEFYHNNLEWINKEANE 1080
            +RVA CGH LHRDCLRFYGFGR VACF YA+I+V SV++PPS +EFY++N EWI KEA+E
Sbjct: 1021 NRVATCGHSLHRDCLRFYGFGRMVACFSYASIHVHSVYLPPSKLEFYYDNQEWIQKEADE 1080

Query: 1081 VHNRAETLFAEVCKAL-PLIPKHEL-GSEQEDFGVNELNHYIQELEQIIIMEKEDFEVHF 1140
            + +RAE LF E+  AL  ++ K  L G++       E +H I ELE+++  E+EDFE   
Sbjct: 1081 MGHRAELLFTELRNALNQILGKRPLAGTQDGGKKAPESSHQIAELEEMLQKEREDFEESL 1140

Query: 1141 NEVLSGETTVGQPTVDIFEINKLHMHILFLSYIWVQRLSYAVTLRRKNSPYSSSNDLLTL 1200
             +V+  E   G P +DI EINKL   +LF SY+W QRL +A +L  K      S+ L  L
Sbjct: 1141 RKVMHREVKFGHPAIDILEINKLRRQLLFHSYVWDQRLIHAASLSNKGFQEGLSSSLPKL 1200

Query: 1201 DERGIESVEEFVEFTLGLGQDDFFGGCDNDSLPLESKHEIDLGRGGNSGQISQLNETERE 1260
             E+ + S+E+  E  +    D         S PLES      G+                
Sbjct: 1201 KEKPLSSMEKLAETNINSKPD--------KSDPLES------GK---------------- 1260

Query: 1261 KDIDLGVLEDDLCLSPVMPQDVEISMRRANSEG-EYPIMADLSDTLDAVWKGVMHPNSA- 1320
                                    S+RRA SEG E P +A+LSDTLDA W G  HP S  
Sbjct: 1261 ------------------------SVRRALSEGDECPTVANLSDTLDAAWTGESHPTSTI 1320

Query: 1321 TSDSSYSRTNSAI------LEPLVGQSDMEKCNANLVGAETARLLHSALVLTMVDSVETS 1380
              D+ YS  +S +      +  +   SD++    + VG +    L S L L         
Sbjct: 1321 PKDNGYSIPDSTLVNSPTAIRKVASNSDLQNYTIDQVGVQVTHSLSSPLHL--------- 1380

Query: 1381 TSLVSMPSSAYNTKISLLNIPKPDIGNYDPAFISSFRELEKQGYLRLLMHVGSTDTVVPV 1440
                      ++  IS LN  K  IG  +P ++  FRELE+Q   RLL+ +G  DTV+PV
Sbjct: 1381 --------KGFDKNIS-LNAQKLFIGEGNPVYVPLFRELERQSGARLLLPIGVNDTVIPV 1440

Query: 1441 YDEEPTSIIAYSLLTPEYNAQMSEPERVLEPVNTARSLPIIGSAKMNSIKTCDEEATDAY 1500
            +D+EPTSIIAY+L++P+Y+ Q+SE ER  + ++++ SLP+  SA + S+ + DE  ++ Y
Sbjct: 1441 FDDEPTSIIAYALVSPDYHLQISESERPKDALDSSVSLPLFDSANLLSLTSFDEAVSETY 1500

Query: 1501 QTLRSKEETILSMPRPESLQFEDLLLFTQDLHTRVCFVNETPLGPVKYTITCYFAKRFEA 1560
            + L S +E+++S  R  S Q  D LL ++DLH RV F ++ PLG VKYT+TCY+A RFEA
Sbjct: 1501 RNLGSSDESLISTSRSRSSQALDSLL-SKDLHARVSFTDDGPLGKVKYTVTCYYATRFEA 1560

Query: 1561 LRKKCCPSELDYVRSISRCRRWGAQGGKSNVFFAKTLDERFIIKQVTKIELESFFQFAPA 1620
            LR+ CCPSE+D+VRS+SRC++WGAQGGKSNVFFAKTLD+RFIIKQVTK ELESF +FAP+
Sbjct: 1561 LRRTCCPSEIDFVRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFAPS 1620

Query: 1621 YFIYLSEAIRTGCPTCLAKVVGMYQVSSKHPKGGRECKLDILVMENLLFRHNVTRLYDLK 1680
            YF YLSE+I T  PTCLAK++G+YQVSSKH KGG+E K+D+LVMENLLFR NVTRLYDLK
Sbjct: 1621 YFKYLSESISTRSPTCLAKILGIYQVSSKHGKGGKESKMDVLVMENLLFRRNVTRLYDLK 1680

Query: 1681 GSSRSRYNSDTSGKNKVLLDQNLIESMPTSPIFLGSKAKRLLERAVWNDTSFLASIDVMD 1740
            GSSRSRYN DTSG NKVLLDQNLIE+MPTSPIF+G+KAKRLLERAVWNDT+FLASIDVMD
Sbjct: 1681 GSSRSRYNPDTSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLASIDVMD 1740

Query: 1741 YSLLVGLDEEKQELVVGIIDFMRQYTWDKHLETWVKNSGILGGPKNAAPTVISPHQYKKR 1764
            YSLLVG+DEEK ELV+GIIDF+RQYTWDKHLETWVK SG+LGGPKN +PTVISP QYKKR
Sbjct: 1741 YSLLVGVDEEKDELVLGIIDFVRQYTWDKHLETWVKTSGLLGGPKNTSPTVISPQQYKKR 1760