Carg16358 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg16358
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Lipase
Location	Cucurbita_argyrosperma_scaffold_088 : 466729 .. 471823 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAAGCCATTGATGGCTATGGTATTTCTTCTTTGCTTCTTCGCTCTCCATTCTGCCGATCATTCCTCTTATCGTTCCCTTGAGTACTCGATTCTTCCTCGCGGTCTGTCTGACCAGAAGTCTTTGTGCTCTCAACTTGTTCAACCTGCTGGTTATCCTTGCGCGGAGCATCAGGTGCTGTGCTTCTCTCATCTCGGTTCTGATTTTTTGGTTGATTGATTAGTGGAAGTAGTGGATTAGGAATTGTTTGTGGATGTTAGTGTTCATTGCTCTGTTTTGACTGGAGGAACTGGAGCGGATTTAGTCAACTAATTGGCTCTAATGCTTCTTGTTTGAGAGTTTTTTTCTCTCTCTTTCCTGAGGTGAGAATGATGGAATGATTTTTCATTTAATTCTTAGATTCGATGTCCGTCCTGGTTTGATTTGTTTCTGTCGAATTTCTGGATAGTCCGATGCGTTTGTATTGTTCTTTGTGTTCATGATGGAAAGTATGAGTACAGAGAAAATACGCGAAGGAAAATGACCGTATTGGCTTGATTTTATCAAATTTTCATTAGCTATTCGGAGGCTCAAGCATTTGTAGCGTATGTTGGATAATGAAAGTCCCATGTTGGCTAGTTTAGGGAATGATCGTGAGTTTATAATCAAAGAATACTATCTCTATTGGTACGAAACCTTTTGGGGAAGCCCAAAGCAAAGCCATGGGAGCTTATGCTCAAAGTGGATAATATCATACCATTGTGGAGAGTCATGTTTGTTGCATATACATATGTATTTTTAAATAGTATCTTCTTTTTATCACTCTCTTGAATTCTGAGGAATGCTATTCATTATTGCAGACTGGGATGATGAATTGTGGTTTTAGTGGCGTACTTAATTACGGTTGTGCATTACTGACGGACGTGATTTGGTTATAAATTTCTTAGATACAAACGAAGGATGGATTTTTATTGGGTCTTCAACGTGTATCTTCATGCGATGGAGATTTGGAAAAGCAAAAGGGCCCTCCAATTTTGCTACTCCATGGACTGTTCATGGTGAGTCTTCCTACAGTTTTGCAACTGTATTTGTAAAATTACTTTACTCTTTATGGTTCAACCCTATCAGCGATAGTTGGAAAGTTTTCAAGGTTGCCTGATATTTTGATTATACGATTGAGACTTATCTCGTTTCTTTGAAATTTTAAACTGCATCATTTCTACAAATTCCAGTAAAATGATTAGTTTTCAAGAAAAAGTAATTATGGTTTTCTTTTAGGTCAGTATTAACCTTAAACTTCTACAGTCCAATTGTTCAAACATGCCTTTGAGTTCGCTTAAGGAATGGTATTGTAAGATGAATGACATAAAGGCTCCTGGAAAATTTTTAGCCAAGAAAGTATCTTATACTTCTGTCTTTCTTTTTCTTTTTTTCCCTTGTTATTCTTCTCCCTTCTGTTTGCTGCACATTTCAAAGCATTTTGAAACATGGATTGTGAATTTTGAATAATCTGTAGACTGGGATCATTCTTACAGATAGGAGTTCCAACTACTAGTTTTTTATTTCTTTCATTTCCTGAACTGGTGCTCGTGCAGGGTGGCGATGCATGGTTTTTGAATTCTGCAAGAGAATCTTTAGGATTTATCCTTGCAGATAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACTCGGTGGAGTCATGGGCATACATCCCTATCAGAGGATGAGAAGGTACTTATATTACTTTATTATAATATGCCTAAGGTGGGTAAGATGCTATTAAATGCAGAGTAAGAATATATTAATAGAAAATTTCTTGATTGTATTATGTGTATATTGGATGTATCTAGAAAGTTATATAACATACAAACATGCTGCTGCTCATCGTGCTAAATGTGTTAATCAATGTGTTGAGGTGGAGAGTATCATACAGTTAATTTTGGAGGTTCAAACAAATTTGAGTGAGTTTTTTCGGAGTTTTGCAGTTACATGACAGATTATATTTAAGAACCATTTGTTTCATGCTAACTGTGTTCATTTGCTAAAGCCTATTTTGTCTCGAACCTCTGCACTATATTGACAGTACTTTAACATAAGTTGAATATGTTTAGTTTCTGGTTACGTTTTTGACTCTAGCATATTCTGATTGTAGGAGTTTTGGGATTGGAGTTGGGAAGACTTGGCTTTATATGATCTTGCAGGCATGATTGACTACATAAATTCATTGACAAAGAGGAAACTTTATGTTGTTGGGCACTCACAGGTCTCATACACCCTCTTTTATAAGATTCTTTGGTGAGGATTGTTGCGAGGGATCCAAGTTGGCTAGGATTTATAAGTAAGGAATATGTCTCCATTGGTACGAGGCCTTTTGGGGAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGATTCCTAACATTCTTTATCTGAAATTTTTTATCGTTGCTTTTGTGATGTTTTTGTCTTTTTTCGCAGGGAACAATTATGTCTTTTGCTGCTCTTACCCAGCCAGACATAGCCGAGAAAGTTGAAGCAGCTGCTCTTTTAAGTCCCATATCGTACTTGGAGCATATCACTGCTCCATTAGTACGTCTAATGGTTGACACACATCTCGATACGGTATTCCTGGCTTGTTATTGTGTAATTAGCATTGTTAAATGTATTATTACTAGTAATAAGCTTGTTCCTATATTTTTCTCGGCAGATAATTCTTGCCGCGGGATTCCATGAACTCAACTTTAAAAGGTTGTCATCCTATGCTAAGTCCAATTTTGATTCTGTAATTGGTTGCATTTTTAGCTTCAGTATAATTCATGACACTAATGTCTATCTTTTGTACCCTACTTCAGTGATTGGGGAACAGTCCTTTTAGATGATCTTTGTGATCGGCTCGTTAACTGCATCAATGTTCTAAGTTCAATAACAGGTTCTCTAAATGCAAAGCACATCATGTTTCATTCTCTTTTCTTATTTTTCTTCTGTTTGGCTTCCAATTCAGATAGGAAATTCGTGCTACTTTTTGATTATTTAGTTAATTGTCTAGCAATTCAAGAAATGGACTACCTACTAGCTAAGCTTCCATTTTTTACTCTCATGCATATCTGCATTTTCATCCATTTTGTTGAACTTAACTTTATTTACATCTTTTTCAGAATTCAAATGCCCTTGGATGGGAAAATTTCAATTATGGTACTTAAAAAAATGTCGTGATTCAATAATTATTGATGTCAGGATTGTCATGTTTCTTCTTGTAGTCAAAAAAATTTGGGTTACAACTAGAAAAGAAACTCGATAATGAGCTTTTGACTGGAAAGGCTACAAAATGTTGCTTTGTTCTTTATAGGAAAGTATCAAGTTTGAATGTTCAATCTATTCAATTGCAGACGTTAAATTTTCTAACAAGGCCTTCTTATAATGTGGCAGGTGAAAATTGTTGCCTTAATGGCTCACGGTTCGACTTATTTTTCGAATACGAACCACATCCATCATCAGCAAAAAACTTGCACCATCTTTTCCAAAGTACGCTCTGCTGTATCTTCTTTAGCTTCATGTTCTATTATACTATGATTTCGATGAATCTACAAGTTTTCACTTGCACATCTGTTGGGAAGTTACTTTTAGGTGTTCCCTTGTGAAGTCTAATATAAATCCTTAAAACATTTGATTGTGAAACTTTGTGAGACTTCTCTAGGGAAGTTACTTTTAGTGTTCCCGTGAGACTACTCCATAAAGGGATTTCCATATGCTCTTTAATCTCTTTCCTGAGCGACTTTTCATCTCCAATAAATCCCTCGCTTTCCAGTTTTCATCAACTTTGTCTCTAATTAAAGCTACCCCTCCAGCTGCTCAAGAAATTCCAGACCATCATCCCCTTATAAAAGCCCGAGGATCCTCCCATCCTTGCTTGAATTTCTATACCTTCCCTTTCCACCCTTCCCCTGTTCCTTTAAATCAAAGCTCCAATTCTGCTTGACGTTTTAAAATCTTCTTATTTCCTTTAACGCAACACGACCAAATGTGAGATCCCACATCAGTTGGGGAGGAGAACAAAACACCCTTTATAAGAGTGTGGAAACCTCTCCCTAGCAGACGCGTTTTAAAAACCTTGAGGGGAAGCCCGAAAGGGAAAGCCCAAAGAGAACAATATCTGCTAGCGGTGGGCTTGGGCTGTGACACCGAACTTCGGTTATTATTCCCAAGCTGTTTTCTAACGTGAGTCATCTTTGTCGTATTGTAATAAAGTGAGAACATTACTAATGATTTAGGATCAGTTTTTCTGTTGTTAAATCTGTTGATACTCTAGAATTTGAAAGAAGAAGAATTAAGCAAGAATTTGTAGTTCATTGTCGTATTAATGAACTGACAAAACTGTTAGAAATCACAACTCTCCACGATGGTATAATATTGTCTACTTTGAGCATAAGCTCTCATGACTTTGCTTTGGGTTTCCCCAATAGGTTTCGTACCAATAGAGATGTATTCTTTACTTGTAAACCCATGATCAATTCTCAACAAAAACTTCTCCTTAAAAGCAAAACCGATCCTAAACCATCACTAATATCCTCACTGAAATTGAATGTTTTTCTCCTCCTGGAGGGAATGGGGAGGCTGTTCGATACAGCTGATTTAACTGCTGCCTTCCTTTTCTTTTACCAGTGATCCGCAAGGGTACTTTTTCCAGGTATGACTATGGATTGTTAAAGAATCTGAGAGTCTATGGCCAAAGGAAGCCTCCTGCATTTGATCTCAGCCGTATTCCTAAGTCCTTGCCGCTGTGGATGGCGTACGGGGGCAGCGACGAGTTGTCGGATTGGACAGATCTGCAGCACACGATCAAGGAGCTGAAGTCGGTACCAGAATTGGTTTACCTTGAAAATTATGGCCATGTTGACTTCATTTTGAGCATGAAAGCGAAGGAAGATGTGTATGATCCTATGATCAAATTCTTGAAATCCTTGGGAAAGTCAGGTAGTTTGTAAAATACAAAATGAGTTCTGGTTAATGTTATTCATTGTAAATATGATGTATGGGGAGTTAAATGTTGTATTTATGTATGTATTAGTTCTTGAA

mRNA sequence

ATGGCGAAGCCATTGATGGCTATGGTATTTCTTCTTTGCTTCTTCGCTCTCCATTCTGCCGATCATTCCTCTTATCGTTCCCTTGAGTACTCGATTCTTCCTCGCGGTCTGTCTGACCAGAAGTCTTTGTGCTCTCAACTTGTTCAACCTGCTGGTTATCCTTGCGCGGAGCATCAGATACAAACGAAGGATGGATTTTTATTGGGTCTTCAACGTGTATCTTCATGCGATGGAGATTTGGAAAAGCAAAAGGGCCCTCCAATTTTGCTACTCCATGGACTGTTCATGGGTGGCGATGCATGGTTTTTGAATTCTGCAAGAGAATCTTTAGGATTTATCCTTGCAGATAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACTCGGTGGAGTCATGGGCATACATCCCTATCAGAGGATGAGAAGCATATCACTGCTCCATTAGTACGTCTAATGGTTGACACACATCTCGATACGATAATTCTTGCCGCGGGATTCCATGAACTCAACTTTAAAAGTGATTGGGGAACAGTCCTTTTAGATGATCTTTGTGATCGGCTCGTTAACTGCATCAATGTTCTAAGTTCAATAACAGGTGAAAATTGTTGCCTTAATGGCTCACGGTTCGACTTATTTTTCGAATACGAACCACATCCATCATCAGCAAAAAACTTGCACCATCTTTTCCAAATGATCCGCAAGGGTACTTTTTCCAGGTATGACTATGGATTGTTAAAGAATCTGAGAGTCTATGGCCAAAGGAAGCCTCCTGCATTTGATCTCAGCCGTATTCCTAAGTCCTTGCCGCTGTGGATGGCGTACGGGGGCAGCGACGAGTTGTCGGATTGGACAGATCTGCAGCACACGATCAAGGAGCTGAAGTCGGTACCAGAATTGGTTTACCTTGAAAATTATGGCCATGTTGACTTCATTTTGAGCATGAAAGCGAAGGAAGATGTGTATGATCCTATGATCAAATTCTTGAAATCCTTGGGAAAGTCAGGTAGTTTGTAAAATACAAAATGAGTTCTGGTTAATGTTATTCATTGTAAATATGATGTATGGGGAGTTAAATGTTGTATTTATGTATGTATTAGTTCTTGAA

Coding sequence (CDS)

ATGGCGAAGCCATTGATGGCTATGGTATTTCTTCTTTGCTTCTTCGCTCTCCATTCTGCCGATCATTCCTCTTATCGTTCCCTTGAGTACTCGATTCTTCCTCGCGGTCTGTCTGACCAGAAGTCTTTGTGCTCTCAACTTGTTCAACCTGCTGGTTATCCTTGCGCGGAGCATCAGATACAAACGAAGGATGGATTTTTATTGGGTCTTCAACGTGTATCTTCATGCGATGGAGATTTGGAAAAGCAAAAGGGCCCTCCAATTTTGCTACTCCATGGACTGTTCATGGGTGGCGATGCATGGTTTTTGAATTCTGCAAGAGAATCTTTAGGATTTATCCTTGCAGATAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACTCGGTGGAGTCATGGGCATACATCCCTATCAGAGGATGAGAAGCATATCACTGCTCCATTAGTACGTCTAATGGTTGACACACATCTCGATACGATAATTCTTGCCGCGGGATTCCATGAACTCAACTTTAAAAGTGATTGGGGAACAGTCCTTTTAGATGATCTTTGTGATCGGCTCGTTAACTGCATCAATGTTCTAAGTTCAATAACAGGTGAAAATTGTTGCCTTAATGGCTCACGGTTCGACTTATTTTTCGAATACGAACCACATCCATCATCAGCAAAAAACTTGCACCATCTTTTCCAAATGATCCGCAAGGGTACTTTTTCCAGGTATGACTATGGATTGTTAAAGAATCTGAGAGTCTATGGCCAAAGGAAGCCTCCTGCATTTGATCTCAGCCGTATTCCTAAGTCCTTGCCGCTGTGGATGGCGTACGGGGGCAGCGACGAGTTGTCGGATTGGACAGATCTGCAGCACACGATCAAGGAGCTGAAGTCGGTACCAGAATTGGTTTACCTTGAAAATTATGGCCATGTTGACTTCATTTTGAGCATGAAAGCGAAGGAAGATGTGTATGATCCTATGATCAAATTCTTGAAATCCTTGGGAAAGTCAGGTAGTTTGTAA

Protein sequence

MAKPLMAMVFLLCFFALHSADHSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQIQTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEKHITAPLVRLMVDTHLDTIILAAGFHELNFKSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIKELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKSGSL

Homology

[Close]

BLAST of Carg16358 vs. NCBI nr
Match: XP_022952782.1 (triacylglycerol lipase 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 671.4 bits (1731), Expect = 1.6e-189
Identity = 336/406 (82.76%), Postives = 337/406 (83.00%), Query Frame = 0


Query: 1   MAKPLMAMVFLLCFFALHSADHSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSAD SSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSLSEDEK------------------------------------- 180
           VWIGNVRGTRWSHGHTSLSEDEK                                     
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 -------------------------------HITAPLVRLMVDTHLDTIILAAGFHELNF 240
                                          HITAPLVRLMVDTHLDTIILAAGFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 339
           IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

[Close]

BLAST of Carg16358 vs. NCBI nr
Match: XP_022952781.1 (triacylglycerol lipase 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 669.5 bits (1726), Expect = 6.1e-189
Identity = 335/404 (82.92%), Postives = 335/404 (82.92%), Query Frame = 0


Query: 1   MAKPLMAMVFLLCFFALHSADHSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSAD SSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSLSEDEK------------------------------------- 180
           VWIGNVRGTRWSHGHTSLSEDEK                                     
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 -------------------------------HITAPLVRLMVDTHLDTIILAAGFHELNF 240
                                          HITAPLVRLMVDTHLDTIILAAGFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 337
           IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

[Close]

BLAST of Carg16358 vs. NCBI nr
Match: XP_022952784.1 (triacylglycerol lipase 1 isoform X3 [Cucurbita moschata])

HSP 1 Score: 667.2 bits (1720), Expect = 3.0e-188
Identity = 334/403 (82.88%), Postives = 334/403 (82.88%), Query Frame = 0


Query: 1   MAKPLMAMVFLLCFFALHSADHSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSAD SSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSLSEDEK------------------------------------- 180
           VWIGNVRGTRWSHGHTSLSEDEK                                     
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 -------------------------------HITAPLVRLMVDTHLDTIILAAGFHELNF 240
                                          HITAPLVRLMVDTHLDTIILAAGFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 336
           IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

[Close]

BLAST of Carg16358 vs. NCBI nr
Match: XP_023553815.1 (triacylglycerol lipase 1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 658.3 bits (1697), Expect = 1.4e-185
Identity = 330/404 (81.68%), Postives = 332/404 (82.18%), Query Frame = 0


Query: 1   MAKPLMAMVFLLCFFALHSADHSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSAD SSYRSLEYSILPR LSDQKSLCSQLVQP GYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSS DG+LEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSLSEDEK------------------------------------- 180
           VWIGNVRGTRWSHGHTS+SEDEK                                     
Sbjct: 121 VWIGNVRGTRWSHGHTSVSEDEKEFWDWSWEDLALYDLAGMIDYINSLTKRKIYVVGHSQ 180

Query: 181 -------------------------------HITAPLVRLMVDTHLDTIILAAGFHELNF 240
                                          HITAPLVRLMVDTHLDTIILAAGFHELNF
Sbjct: 181 GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 337
           IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

[Close]

BLAST of Carg16358 vs. NCBI nr
Match: XP_022972380.1 (triacylglycerol lipase 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 656.4 bits (1692), Expect = 5.4e-185
Identity = 328/404 (81.19%), Postives = 329/404 (81.44%), Query Frame = 0


Query: 1   MAKPLMAMVFLLCFFALHSADHSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60
           MAKPLMAMVFLLCFFALHSA+ SSYRSLEYSILPR LSDQKSLCSQLVQPAGYPCAEHQI
Sbjct: 1   MAKPLMAMVFLLCFFALHSANDSSYRSLEYSILPRALSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
           QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSA ESLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSATESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHTSLSEDEK------------------------------------- 180
           VWIGNVRGTRWSHGHTSLSEDEK                                     
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINPLTKRKIYVVGHSQ 180

Query: 181 -------------------------------HITAPLVRLMVDTHLDTIILAAGFHELNF 240
                                          HITAPLVRLMVDTHLDTIILAAGFHELNF
Sbjct: 181 GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGT LLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTALLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 337
           IRKGTFSRYDYGL KNLRVYGQRKPPAFDLS IPKSLPLWMAYGGSDELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLFKNLRVYGQRKPPAFDLSHIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

[Close]

BLAST of Carg16358 vs. TAIR10
Match: AT2G15230.1 (lipase 1)

HSP 1 Score: 374.0 bits (959), Expect = 9.7e-104
Identity = 186/381 (48.82%), Postives = 235/381 (61.68%), Query Frame = 0


Query: 25  YRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQIQTKDGFLLGLQRVSSCDGDLEKQK 84
           + +L  S L  G S   SLC+ L+ PA Y C EH IQTKDG++L LQRV+S    L  Q 
Sbjct: 15  FSALTQSHLLHG-SPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRL--QS 74

Query: 85  GPPILLLHGLFMGGDAWFLNSARESLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEK- 144
           GPP+LL HGLFM GD WFLNS +ESLGFILAD+GFDVW+GNVRGTR+S+GH +LS+ +K 
Sbjct: 75  GPPVLLQHGLFMAGDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKE 134

Query: 145 ------------------------------------------------------------ 204
                                                                       
Sbjct: 135 FWDWSWQDLAMYDLAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALL 194

Query: 205 -------HITAPLVRLMVDTHLDTIILAAGFHELNFKSDWGTVLLDDLCDRLVNCINVLS 264
                  H+TAPLV  MV  HLD +++A G H++NF+SD    L+D LC+  ++C + L+
Sbjct: 195 CPISYLDHVTAPLVERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLT 254

Query: 265 SITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRK 324
           SITG NCC N S+ + + +YEPHPSS KN+ HLFQMIRKGTF++YDYG  KNLR YG  K
Sbjct: 255 SITGTNCCFNASKIEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSK 314

Query: 325 PPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIKELKSVPELVYLENYGHVDFILSMK 338
           PP F LS IP SLP+WM YGG+D L+D TD++HT+ EL S PEL+YLE+YGH+DF+L   
Sbjct: 315 PPEFILSHIPASLPMWMGYGGTDGLADVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSS 374

[Close]

BLAST of Carg16358 vs. TAIR10
Match: AT5G14180.1 (Myzus persicae-induced lipase 1)

HSP 1 Score: 193.7 bits (491), Expect = 1.8e-49
Identity = 117/367 (31.88%), Postives = 185/367 (50.41%), Query Frame = 0


Query: 43  LCSQLVQPAGYPCAEHQIQTKDGFLLGLQRV-----SSCDGDLEKQKGPPILLLHGLFMG 102
           +C+  V   GY C EH + T+DG++L +QR+      +  GD  K++  P+L+ HG+ + 
Sbjct: 51  ICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQ--PVLIQHGILVD 110

Query: 103 GDAWFLNSARESLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEK-------------- 162
           G +W LN A ++L  ILAD GFDVW+GN RGTR+S  H  L+  ++              
Sbjct: 111 GMSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYD 170

Query: 163 --------------------HITAPLV-------RLMVD--------------THLDTII 222
                               H    L+       + +VD              +H+ T+I
Sbjct: 171 LPAMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVI 230

Query: 223 --LAA-----------GFHELNFKSDWGTVLLDDLCDRL-VNCINVLSSITGENCCLNGS 282
             +AA           G+ E N KS      +  +C +  ++C +++S ITG+NCCLN S
Sbjct: 231 GDIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNAS 290

Query: 283 RFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLL-KNLRVYGQRKPPAFDLSRIPK 331
             DLF   EP  +S KN+ HL Q +R     +Y+YG   +N++ YGQ  PPA+++S IP 
Sbjct: 291 TIDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPH 350

[Close]

BLAST of Carg16358 vs. TAIR10
Match: AT1G73920.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 52.8 bits (125), Expect = 4.9e-07
Identity = 26/85 (30.59%), Postives = 39/85 (45.88%), Query Frame = 0


Query: 44  CSQLVQPAGYPCAEHQIQTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFL 103
           C  ++   GYP    ++ T DG++L L+R+   D          + L HG+      W  
Sbjct: 296 CQDVITELGYPYEAIRVITSDGYVLVLERIPRRDA------RKAVFLQHGVLDSSMGWVS 355

Query: 104 NSARESLGFILADNGFDVWIGNVRG 129
           N    S  F   D G+DV++GN RG
Sbjct: 356 NGVVGSPAFAAYDQGYDVFLGNFRG 374

[Close]

BLAST of Carg16358 vs. TAIR10
Match: AT1G18460.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 50.4 bits (119), Expect = 2.4e-06
Identity = 26/85 (30.59%), Postives = 38/85 (44.71%), Query Frame = 0


Query: 44  CSQLVQPAGYPCAEHQIQTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFL 103
           C  ++   GYP    ++ T DG+ L L+R+   D          + L HG+      W  
Sbjct: 294 CQDVITELGYPYEAIRVVTSDGYGLLLERIPRRDA------RKAVYLQHGVMDSSMGWVS 353

Query: 104 NSARESLGFILADNGFDVWIGNVRG 129
           N    S  F   D G+DV++GN RG
Sbjct: 354 NGVVGSPAFAAYDQGYDVFLGNFRG 372

[Close]

BLAST of Carg16358 vs. Swiss-Prot
Match: sp|Q71DJ5|LIP1_ARATH (Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 1.7e-102
Identity = 186/381 (48.82%), Postives = 235/381 (61.68%), Query Frame = 0


Query: 25  YRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQIQTKDGFLLGLQRVSSCDGDLEKQK 84
           + +L  S L  G S   SLC+ L+ PA Y C EH IQTKDG++L LQRV+S    L  Q 
Sbjct: 15  FSALTQSHLLHG-SPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRL--QS 74

Query: 85  GPPILLLHGLFMGGDAWFLNSARESLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEK- 144
           GPP+LL HGLFM GD WFLNS +ESLGFILAD+GFDVW+GNVRGTR+S+GH +LS+ +K 
Sbjct: 75  GPPVLLQHGLFMAGDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKE 134

Query: 145 ------------------------------------------------------------ 204
                                                                       
Sbjct: 135 FWDWSWQDLAMYDLAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALL 194

Query: 205 -------HITAPLVRLMVDTHLDTIILAAGFHELNFKSDWGTVLLDDLCDRLVNCINVLS 264
                  H+TAPLV  MV  HLD +++A G H++NF+SD    L+D LC+  ++C + L+
Sbjct: 195 CPISYLDHVTAPLVERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLT 254

Query: 265 SITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRK 324
           SITG NCC N S+ + + +YEPHPSS KN+ HLFQMIRKGTF++YDYG  KNLR YG  K
Sbjct: 255 SITGTNCCFNASKIEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSK 314

Query: 325 PPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIKELKSVPELVYLENYGHVDFILSMK 338
           PP F LS IP SLP+WM YGG+D L+D TD++HT+ EL S PEL+YLE+YGH+DF+L   
Sbjct: 315 PPEFILSHIPASLPMWMGYGGTDGLADVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSS 374

[Close]

BLAST of Carg16358 vs. Swiss-Prot
Match: sp|Q67ZU1|LIP2_ARATH (Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 3.2e-48
Identity = 117/367 (31.88%), Postives = 185/367 (50.41%), Query Frame = 0


Query: 43  LCSQLVQPAGYPCAEHQIQTKDGFLLGLQRV-----SSCDGDLEKQKGPPILLLHGLFMG 102
           +C+  V   GY C EH + T+DG++L +QR+      +  GD  K++  P+L+ HG+ + 
Sbjct: 51  ICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQ--PVLIQHGILVD 110

Query: 103 GDAWFLNSARESLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEK-------------- 162
           G +W LN A ++L  ILAD GFDVW+GN RGTR+S  H  L+  ++              
Sbjct: 111 GMSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYD 170

Query: 163 --------------------HITAPLV-------RLMVD--------------THLDTII 222
                               H    L+       + +VD              +H+ T+I
Sbjct: 171 LPAMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVI 230

Query: 223 --LAA-----------GFHELNFKSDWGTVLLDDLCDRL-VNCINVLSSITGENCCLNGS 282
             +AA           G+ E N KS      +  +C +  ++C +++S ITG+NCCLN S
Sbjct: 231 GDIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNAS 290

Query: 283 RFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLL-KNLRVYGQRKPPAFDLSRIPK 331
             DLF   EP  +S KN+ HL Q +R     +Y+YG   +N++ YGQ  PPA+++S IP 
Sbjct: 291 TIDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPH 350

[Close]

BLAST of Carg16358 vs. Swiss-Prot
Match: sp|P04634|LIPG_RAT (Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 5.2e-30
Identity = 93/360 (25.83%), Postives = 156/360 (43.33%), Query Frame = 0


Query: 45  SQLVQPAGYPCAEHQIQTKDGFLLGLQRVSSCDGDLEK-QKGPPILLLHGLFMGGDAWFL 104
           SQ++   GYPC E+++ T+DG++LG+ R+     + E   K P + L HGL      W  
Sbjct: 35  SQMITYWGYPCQEYEVVTEDGYILGVYRIPHGKNNSENIGKRPVVYLQHGLIASATNWIA 94

Query: 105 NSARESLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEKHITA----PLVRLMVDTHLD 164
           N    SL F+LAD G+DVW+GN RG  WS  +   S D     A     + +  +   ++
Sbjct: 95  NLPNNSLAFMLADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATIN 154

Query: 165 TIILAAGFHELN------------------------------------------------ 224
            I+   G  +++                                                
Sbjct: 155 FIVQKTGQEKIHYVGHSQGTTIGFIAFSTNPTLAKKIKTFYALAPVATVKYTQSPLKKIS 214

Query: 225 ------FKSDWGTVLL-----------DDLCDRLV---NCINVLSSITG-ENCCLNGSRF 284
                 FK  +G  +             ++C R V    C N L    G +   LN SRF
Sbjct: 215 FIPTFLFKLMFGKKMFLPHTYFDDFLGTEVCSREVLDLLCSNTLFIFCGFDKKNLNVSRF 274

Query: 285 DLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGL-LKNLRVYGQRKPPAFDLSRIPKSL 330
           D++  + P  +S ++  H  Q++R G F  +++G   +N+  Y Q+ PP +D+S +   +
Sbjct: 275 DVYLGHNPAGTSVQDFLHWAQLVRSGKFQAFNWGSPSQNMLHYNQKTPPEYDVSAMTVPV 334

[Close]

BLAST of Carg16358 vs. Swiss-Prot
Match: sp|Q29458|LIPG_BOVIN (Gastric triacylglycerol lipase OS=Bos taurus OX=9913 GN=LIPF PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 9.8e-29
Identity = 92/360 (25.56%), Postives = 153/360 (42.50%), Query Frame = 0


Query: 45  SQLVQPAGYPCAEHQIQTKDGFLLGLQRVSSCDGDLEK-QKGPPILLLHGLFMGGDAWFL 104
           SQ++   GYP   H++ T DG++L + R+     +     + P + L HGL      W  
Sbjct: 35  SQMISYWGYPSEMHKVITADGYILQVYRIPHGKNNANHLGQRPVVFLQHGLLGSATNWIS 94

Query: 105 NSARESLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEKHITA----PLVRLMVDTHLD 164
           N  + SLGF+LAD G+DVW+GN RG  W+  H   S D     A     +    + + +D
Sbjct: 95  NLPKNSLGFLLADAGYDVWLGNSRGNTWAQEHLYYSPDSPEFWAFSFDEMAEYDLPSTID 154

Query: 165 TIILAAGFHELN------------------------------------------------ 224
            I+   G  +L+                                                
Sbjct: 155 FILRRTGQKKLHYVGHSQGTTIGFIAFSTSPTLAEKIKVFYALAPVATVKYTKSLFNKLA 214

Query: 225 ------FKSDWGTVLL-----------DDLCDRL---VNCINVLSSITG-ENCCLNGSRF 284
                 FK  +G  +             ++C R    V C N L +ITG +N   N SR 
Sbjct: 215 LIPHFLFKIIFGDKMFYPHTFLEQFLGVEMCSRETLDVLCKNALFAITGVDNKNFNMSRL 274

Query: 285 DLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGL-LKNLRVYGQRKPPAFDLSRIPKSL 330
           D++  + P  +S +N  H  Q ++ G F  +D+G   +NL  Y Q  PP ++L+ +   +
Sbjct: 275 DVYIAHNPAGTSVQNTLHWRQAVKSGKFQAFDWGAPYQNLMHYHQPTPPIYNLTAMNVPI 334

[Close]

BLAST of Carg16358 vs. Swiss-Prot
Match: sp|Q9CPP7|LIPG_MOUSE (Gastric triacylglycerol lipase OS=Mus musculus OX=10090 GN=Lipf PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.2e-28
Identity = 96/363 (26.45%), Postives = 156/363 (42.98%), Query Frame = 0


Query: 45  SQLVQPAGYPCAEHQIQTKDGFLLGLQRVSSCDGDLEK-QKGPPILLLHGLFMGGDAWFL 104
           SQ++   GYP  E+++ T+DG++LG+ R+     + E   K P   L HGL      W  
Sbjct: 35  SQMITYWGYPSEEYEVVTEDGYILGVYRIPYGKKNSENIGKRPVAYLQHGLIASATNWIT 94

Query: 105 NSARESLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEKHITA----PLVRLMVDTHLD 164
           N    SL FILAD G+DVW+GN RG  WS  +   S D     A     + +  +   +D
Sbjct: 95  NLPNNSLAFILADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATID 154

Query: 165 TIILAAGFHELNFKS-DWGTVL-------------------------------------- 224
            I+   G  ++++     GT +                                      
Sbjct: 155 FIVQKTGQEKIHYVGHSQGTTIGFIAFSTNPALAKKIKRFYALAPVATVKYTESPFKKIS 214

Query: 225 ---------------------LD-----DLCDRL---VNCINVLSSITG-ENCCLNGSRF 284
                                LD     ++C R    + C N L    G +   LN SRF
Sbjct: 215 LIPKFLLKVIFGNKMFMPHNYLDQFLGTEVCSRELLDLLCSNALFIFCGFDKKNLNVSRF 274

Query: 285 DLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGL-LKNLRVYGQRKPPAFDLSRIPKSL 330
           D++  + P  +S ++L H  Q+ + G    Y++G  L+N+  Y Q+ PP +D+S +   +
Sbjct: 275 DVYLGHNPAGTSTQDLFHWAQLAKSGKLQAYNWGSPLQNMLHYNQKTPPYYDVSAMTVPI 334

[Close]

BLAST of Carg16358 vs. TrEMBL
Match: tr|A0A1S3C115|A0A1S3C115_CUCME (Lipase OS=Cucumis melo OX=3656 GN=LOC103495266 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 2.2e-166
Identity = 299/407 (73.46%), Postives = 317/407 (77.89%), Query Frame = 0


Query: 1   MAKPLMAMVFLLCFFALHSADHSSYRS-LEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQ 60
           MA PLMA+VFL+ FF LHSA  SS  + ++YSILP  LSD KSLCSQLV PAGYPCAEH+
Sbjct: 1   MATPLMALVFLIYFFPLHSAGDSSLPAVVDYSILPHALSDHKSLCSQLVLPAGYPCAEHK 60

Query: 61  IQTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGF 120
           IQTKDGFLLGLQRVSS +GDLEKQKGPPILLLHGLFM GD WFLNSAR+SLGFIL DNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRNGDLEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSHGHTSLSEDEK------------------------------------ 180
           DVWIGNVRGTRWSHGH+SLSEDEK                                    
Sbjct: 121 DVWIGNVRGTRWSHGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNRKIYIVGHS 180

Query: 181 --------------------------------HITAPLVRLMVDTHLDTIILAAGFHELN 240
                                           HITAPLVRLMVDTHLDTIILA+GFHELN
Sbjct: 181 QGTIMSFAALTQPEIAKNVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240

Query: 241 FKSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQ 300
           FKSDWGTVLLD+LCDRLVNCINVLSSITGENCCLN SRFDLFFEYEPHPSSAKNLHHLFQ
Sbjct: 241 FKSDWGTVLLDNLCDRLVNCINVLSSITGENCCLNRSRFDLFFEYEPHPSSAKNLHHLFQ 300

Query: 301 MIRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTI 339
           MIRKG+FS+YDYGLLKNLRVYGQR PP FDLS IP+SLPLWMAYGG+DELSDWTDL+HTI
Sbjct: 301 MIRKGSFSKYDYGLLKNLRVYGQRMPPEFDLSLIPESLPLWMAYGGNDELSDWTDLEHTI 360

[Close]

BLAST of Carg16358 vs. TrEMBL
Match: tr|A0A0A0K4L2|A0A0A0K4L2_CUCSA (Lipase OS=Cucumis sativus OX=3659 GN=Csa_7G072760 PE=3 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.8e-165
Identity = 296/407 (72.73%), Postives = 318/407 (78.13%), Query Frame = 0


Query: 1   MAKPLMAMVFLLCFFALHSADHSSYRS-LEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQ 60
           MA PL+A +FLL FF LHSA  SS+ S ++YSILP  LSD KSLCSQLV PAGYPCAEH+
Sbjct: 1   MAMPLLAFIFLLFFFPLHSAGDSSFPSVVDYSILPHALSDNKSLCSQLVLPAGYPCAEHK 60

Query: 61  IQTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGF 120
           IQTKDGFLLGLQRVSS DG+LEKQKGPPILLLHGLFM GD WFLNSAR+SLGFIL DNGF
Sbjct: 61  IQTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMAGDGWFLNSARQSLGFILPDNGF 120

Query: 121 DVWIGNVRGTRWSHGHTSLSEDEK------------------------------------ 180
           DVWIGNVRGTRWS+GH+SLSEDEK                                    
Sbjct: 121 DVWIGNVRGTRWSYGHSSLSEDEKEFWNWSWEELALYDLAEMINYINSLTNKKIYIVGHS 180

Query: 181 --------------------------------HITAPLVRLMVDTHLDTIILAAGFHELN 240
                                           HITAPLVRLMVDTHLDTIILA+GFHELN
Sbjct: 181 QGTIMSFAALTQPDIAKKVGAAALLSPISYLEHITAPLVRLMVDTHLDTIILASGFHELN 240

Query: 241 FKSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQ 300
           FKSDWGTVLLD+LCDRLVNCIN+LSSITGENCCLN SRFDLFF+YEPHPSSAKNLHHLFQ
Sbjct: 241 FKSDWGTVLLDNLCDRLVNCINILSSITGENCCLNRSRFDLFFKYEPHPSSAKNLHHLFQ 300

Query: 301 MIRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTI 339
           MIRKG+FS+YDYGLLKNLRVYGQR PP FDLSRIP+SLPLWMAYGG+DELSDWTDL++TI
Sbjct: 301 MIRKGSFSKYDYGLLKNLRVYGQRVPPEFDLSRIPESLPLWMAYGGNDELSDWTDLENTI 360

[Close]

BLAST of Carg16358 vs. TrEMBL
Match: tr|A0A1S3BZD6|A0A1S3BZD6_CUCME (triacylglycerol lipase 1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495266 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 1.9e-122
Identity = 224/321 (69.78%), Postives = 237/321 (73.83%), Query Frame = 0


Query: 86  PPILLLHGLFMGGDAWFLNSARESLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEK-- 145
           P   L++     GD WFLNSAR+SLGFIL DNGFDVWIGNVRGTRWSHGH+SLSEDEK  
Sbjct: 12  PRPYLIYMCVCAGDGWFLNSARQSLGFILPDNGFDVWIGNVRGTRWSHGHSSLSEDEKEF 71

Query: 146 ------------------------------------------------------------ 205
                                                                       
Sbjct: 72  WNWSWEELALYDLAEMINYINSLTNRKIYIVGHSQGTIMSFAALTQPEIAKNVEAAALLS 131

Query: 206 ------HITAPLVRLMVDTHLDTIILAAGFHELNFKSDWGTVLLDDLCDRLVNCINVLSS 265
                 HITAPLVRLMVDTHLDTIILA+GFHELNFKSDWGTVLLD+LCDRLVNCINVLSS
Sbjct: 132 PISYLEHITAPLVRLMVDTHLDTIILASGFHELNFKSDWGTVLLDNLCDRLVNCINVLSS 191

Query: 266 ITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKP 325
           ITGENCCLN SRFDLFFEYEPHPSSAKNLHHLFQMIRKG+FS+YDYGLLKNLRVYGQR P
Sbjct: 192 ITGENCCLNRSRFDLFFEYEPHPSSAKNLHHLFQMIRKGSFSKYDYGLLKNLRVYGQRMP 251

Query: 326 PAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIKELKSVPELVYLENYGHVDFILSMKA 339
           P FDLS IP+SLPLWMAYGG+DELSDWTDL+HTIK++KSVPELVYLENYGHVDFILSMKA
Sbjct: 252 PEFDLSLIPESLPLWMAYGGNDELSDWTDLEHTIKKVKSVPELVYLENYGHVDFILSMKA 311

[Close]

BLAST of Carg16358 vs. TrEMBL
Match: tr|A0A2P4GRC7|A0A2P4GRC7_QUESU (Lipase OS=Quercus suber OX=58331 GN=CFP56_62668 PE=3 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 5.8e-119
Identity = 215/396 (54.29%), Postives = 266/396 (67.17%), Query Frame = 0


Query: 10  FLLCFFALHSADHSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQIQTKDGFLLG 69
           F LC F   S+  ++  +   S+ PR  +D  +LC+ L+ PAGYPC+EH +QTKDG+LL 
Sbjct: 45  FFLCLF---SSQIAATPTGIPSLRPRLPND--TLCNHLILPAGYPCSEHTVQTKDGYLLA 104

Query: 70  LQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFDVWIGNVRGT 129
           L RVSS  G++ +Q+GPP+LL HGLFM GDAWFL+S  +SLGFILAD+GFDVW+GNVRGT
Sbjct: 105 LHRVSSRTGNISEQRGPPVLLQHGLFMAGDAWFLDSPEQSLGFILADHGFDVWVGNVRGT 164

Query: 130 RWSHGHTSLSEDEK---------------------------------------------- 189
           RWSHGH SLSE +K                                              
Sbjct: 165 RWSHGHVSLSEKDKDFWDWSWQELALHDLADVICYINSITNTKIFFVGHSQGTIMSLAAF 224

Query: 190 ----------------------HITAPLVRLMVDTHLDTIILAAGFHELNFKSDWGTVLL 249
                                 H++AP V  MV+ HLD +ILA G HELNF+SDWG  L+
Sbjct: 225 TQPDIVEMVEAAALLCPISYLGHVSAPFVLRMVNMHLDQMILAMGIHELNFRSDWGIKLM 284

Query: 250 DDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRY 309
           D +CD  V+C ++LSSITG+NCC N SR D + EYEPHPSS+KNL HLFQMIRKGTFS+Y
Sbjct: 285 DSICDGHVDCNDLLSSITGKNCCFNNSRVDFYLEYEPHPSSSKNLRHLFQMIRKGTFSKY 344

Query: 310 DYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIKELKSVPELV 338
           DYGL KNL++YGQ KPP FDL  IPKSLPLW+AYGG+D+L+D TDLQHT+KEL+S PEL+
Sbjct: 345 DYGLFKNLKLYGQLKPPIFDLGHIPKSLPLWIAYGGNDDLADVTDLQHTLKELESKPELL 404

[Close]

BLAST of Carg16358 vs. TrEMBL
Match: tr|M5VJ53|M5VJ53_PRUPE (Lipase OS=Prunus persica OX=3760 GN=PRUPE_8G135900 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.3e-118
Identity = 211/379 (55.67%), Postives = 254/379 (67.02%), Query Frame = 0


Query: 27  SLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQIQTKDGFLLGLQRVSSCDGDLEKQKGP 86
           S ++  L R    Q+SLC +L++P+GY C+EH+IQTKDG+LLGLQRVSS  GDL  Q GP
Sbjct: 29  SADFYNLGRRRLPQQSLCLELIEPSGYSCSEHKIQTKDGYLLGLQRVSSRSGDLRTQLGP 88

Query: 87  PILLLHGLFMGGDAWFLNSARESLGFILADNGFDVWIGNVRGTRWSHGHTSLSEDEK--- 146
           P+LL HGLFM GDAWFLNS  ES+GF+LAD GFDVW+GNVRGTRWSHGH SLSED+K   
Sbjct: 89  PVLLQHGLFMAGDAWFLNSPEESMGFVLADQGFDVWVGNVRGTRWSHGHVSLSEDDKNFW 148

Query: 147 ------------------------------------------------------------ 206
                                                                       
Sbjct: 149 DWSWQELAEYDLAKMIHHIYSITNSKVFIVGHSQGTIMSLAALTQPDVAEMVEAAALFCP 208

Query: 207 -----HITAPLVRLMVDTHLDTIILAAGFHELNFKSDWGTVLLDDLCDRLVNCINVLSSI 266
                HITA  V  MV+ HLD +ILA G HELNF+SDWG  LLD +CD  V+C ++L+SI
Sbjct: 209 ISYLEHITAKFVLRMVNMHLDQMILAMGIHELNFRSDWGVNLLDSICDGHVDCNDLLTSI 268

Query: 267 TGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRKGTFSRYDYGLLKNLRVYGQRKPP 326
           TG+NCC N SR D +  YEPHP+S KNLHHLFQMIRKGTFS+YDYG+LKNL++YGQ KPP
Sbjct: 269 TGKNCCFNNSRVDFYLNYEPHPTSTKNLHHLFQMIRKGTFSKYDYGILKNLKLYGQLKPP 328

Query: 327 AFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIKELKSVPELVYLENYGHVDFILSMKAK 338
           +FDL  IPKSLPL MAYGG+D+L+D  D  HT+KEL+S PELVYLENYGH+DFI+S+ AK
Sbjct: 329 SFDLGLIPKSLPLLMAYGGNDDLADVEDFHHTLKELQSTPELVYLENYGHIDFIVSINAK 388

The following BLAST results are available for this feature:

BLAST of Carg16358 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022952782.1	1.6e-189	82.76	triacylglycerol lipase 1 isoform X2 [Cucurbita moschata]	[more]
XP_022952781.1	6.1e-189	82.92	triacylglycerol lipase 1 isoform X1 [Cucurbita moschata]	[more]
XP_022952784.1	3.0e-188	82.88	triacylglycerol lipase 1 isoform X3 [Cucurbita moschata]	[more]
XP_023553815.1	1.4e-185	81.68	triacylglycerol lipase 1 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022972380.1	5.4e-185	81.19	triacylglycerol lipase 1 isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg16358 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G15230.1	9.7e-104	48.82	lipase 1	[more]
AT5G14180.1	1.8e-49	31.88	Myzus persicae-induced lipase 1	[more]
AT1G73920.1	4.9e-07	30.59	alpha/beta-Hydrolases superfamily protein	[more]
AT1G18460.1	2.4e-06	30.59	alpha/beta-Hydrolases superfamily protein	[more]

BLAST of Carg16358 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q71DJ5\|LIP1_ARATH	1.7e-102	48.82	Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1	[more]
sp\|Q67ZU1\|LIP2_ARATH	3.2e-48	31.88	Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1	[more]
sp\|P04634\|LIPG_RAT	5.2e-30	25.83	Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1	[more]
sp\|Q29458\|LIPG_BOVIN	9.8e-29	25.56	Gastric triacylglycerol lipase OS=Bos taurus OX=9913 GN=LIPF PE=1 SV=1	[more]
sp\|Q9CPP7\|LIPG_MOUSE	2.2e-28	26.45	Gastric triacylglycerol lipase OS=Mus musculus OX=10090 GN=Lipf PE=2 SV=1	[more]

BLAST of Carg16358 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3C115\|A0A1S3C115_CUCME	2.2e-166	73.46	Lipase OS=Cucumis melo OX=3656 GN=LOC103495266 PE=3 SV=1	[more]
tr\|A0A0A0K4L2\|A0A0A0K4L2_CUCSA	1.8e-165	72.73	Lipase OS=Cucumis sativus OX=3659 GN=Csa_7G072760 PE=3 SV=1	[more]
tr\|A0A1S3BZD6\|A0A1S3BZD6_CUCME	1.9e-122	69.78	triacylglycerol lipase 1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495266 PE=4...	[more]
tr\|A0A2P4GRC7\|A0A2P4GRC7_QUESU	5.8e-119	54.29	Lipase OS=Quercus suber OX=58331 GN=CFP56_62668 PE=3 SV=1	[more]
tr\|M5VJ53\|M5VJ53_PRUPE	1.3e-118	55.67	Lipase OS=Prunus persica OX=3760 GN=PRUPE_8G135900 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0006629	lipid metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR006693	AB_hydrolase_lipase
IPR029058	AB_hydrolase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16358-RA	Carg16358-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	G3DSA:3.40.50.1820		coord: 38..145 e-value: 2.1E-33 score: 117.7 coord: 146..332 e-value: 2.8E-39 score: 137.1
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	SSF53474	alpha/beta-Hydrolases	coord: 44..330
IPR006693	Partial AB-hydrolase lipase domain	PFAM	PF04083	Abhydro_lipase	coord: 45..104 e-value: 1.6E-16 score: 59.5
None	No IPR available	PANTHER	PTHR11005:SF37	TRIACYLGLYCEROL LIPASE 1	coord: 143..335 coord: 26..143
None	No IPR available	PANTHER	PTHR11005	LYSOSOMAL ACID LIPASE-RELATED	coord: 143..335 coord: 26..143

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg16358	Cla016059	Watermelon (97103) v1	carwmB0455
Carg16358	Csa7G072760	Cucumber (Chinese Long) v2	carcuB0488
Carg16358	MELO3C018487	Melon (DHL92) v3.5.1	carmeB0458
Carg16358	ClCG02G006410	Watermelon (Charleston Gray)	carwcgB0445
Carg16358	CSPI07G07250	Wild cucumber (PI 183967)	carcpiB0494
Carg16358	Cucsa.219480	Cucumber (Gy14) v1	carcgyB0525
Carg16358	CmaCh19G005770	Cucurbita maxima (Rimu)	carcmaB0632
Carg16358	CmoCh19G005910	Cucurbita moschata (Rifu)	carcmoB0615
Carg16358	Lsi03G005400	Bottle gourd (USVL1VR-Ls)	carlsiB411
Carg16358	Cp4.1LG15g04680	Cucurbita pepo (Zucchini)	carcpeB0637
Carg16358	CsGy7G006820	Cucumber (Gy14) v2	carcgybB0465
Carg16358	MELO3C018487.2	Melon (DHL92) v3.6.1	carmedB0461
Carg16358	CsaV3_7G008200	Cucumber (Chinese Long) v3	carcucB0491
Carg16358	Cla97C02G033380	Watermelon (97103) v2	carwmbB0477
Carg16358	Bhi05G000977	Wax gourd	carwgoB0549

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg16358	Silver-seed gourd	carcarB228
Carg16358	Silver-seed gourd	carcarB402
Carg16358	Cucumber (Gy14) v2	carcgybB0464
Carg16358	Cucumber (Gy14) v1	carcgyB0524
Carg16358	Cucurbita maxima (Rimu)	carcmaB0630
Carg16358	Cucurbita maxima (Rimu)	carcmaB0634
Carg16358	Cucurbita moschata (Rifu)	carcmoB0612
Carg16358	Cucurbita moschata (Rifu)	carcmoB0614
Carg16358	Cucurbita moschata (Rifu)	carcmoB0616
Carg16358	Cucurbita pepo (Zucchini)	carcpeB0639
Carg16358	Wild cucumber (PI 183967)	carcpiB0493
Carg16358	Cucumber (Chinese Long) v2	carcuB0487
Carg16358	Cucumber (Chinese Long) v3	carcucB0490
Carg16358	Bottle gourd (USVL1VR-Ls)	carlsiB414
Carg16358	Melon (DHL92) v3.5.1	carmeB0460
Carg16358	Melon (DHL92) v3.6.1	carmedB0463
Carg16358	Watermelon (97103) v2	carwmbB0478
Carg16358	Watermelon (97103) v2	carwmbB0480
Carg16358	Wax gourd	carwgoB0552
Carg16358	Wax gourd	carwgoB0555