Carg16281 (gene) Silver-seed gourd

NameCarg16281
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionnuclear RNA polymerase C2
LocationCucurbita_argyrosperma_scaffold_077 : 500393 .. 505184 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTGTAAATGTTATCGGTGAATCTATTAGTTCTCATATGTGCTGCTTATCTGACCTCACGTATGGTTCCTTCGTGAATGTTATTATCATTTAGTTGAATACTGCAGAAAGACGGGGTGACAGGGAGGATGCCTATATGCTATGAAGACGTTGTTGTATTTTACGCAATTTCAAAGTCTCCAATTATATAATTCTTCCGAAGTTTTGCAGGTTTTTTGATGTAAGAATTGGCTAGCCTGACCGTGAATGCTATCAGTGAATCTATTAGTTCTCATATGTGCCGCTTATCTGACCTTACATTATGGTTCCTTTGTGAATGTTATTGCCATTTAGTTGAATATTGCAGAAAGACGTGGTGATTGGGAAGATGCCTATTGGCGAGCCTTTTGTGACCGTGAATGCTGTCAGTGAATCTATTAGTTTTCATATGTGCCGCTTATCTGACCTTACGTATGGTTCCTTCGTGAATGTTATTACCATTTAGTTGAATATTGTAGAAAGACGTGGTGATTGGGAGGATGGCTATATGCTATAAAGACATTGCTGTGTTTTACGCGATTTCAATATTTCCAATTGTATAATTCTTTCGGAGTTTTGCAGGTTCTTTGATGTAAGAATTAGCAAGCCTTGTCCTCATATGTGCCACTTATCTGACCTTATGTATGGTTCGTTCGTGAATGTTATTACCATTTACCATAAAGTAATATTATCTATTTTTATAATTGCACTTAGCTAACACTGTAAAATTCTCCCGTCCATGTCAGGTTGTTTTGATTCAAGAACAACTTTCGAAAAACAGGATTATCATTGATACTGACAAAAGGGGAAGTGAGTACACTTTTCAAGTCACTATTTCTCTTCCATATAATTATGTCTTCTAGGTTCCTAGTTTATTTATTGTCCTTACATAGGATTAGAAATTGATTCTAATGGGCATTGTTTGAGCTTGTAACTAGATCGTGGATCCATATGGTTTTGGATGCTTTTTTGGTTGGTTTATAGTTGTTTTTATAATAATCTCTTGGTGATGTGAGTAAGTTGTTCAATTAGAGTATTATTGAAGGATGCAAGGTGATGCAACCAAGCATCAAAGGATAAAATCTCCAAGAATTCTCAAAAGAACTTTCATTATGATTCAAAAAGCAAGTTAATCGCAAGAGAGATCAACCTTCTATTCTAGTGGATTGATAGGTTTACCTTGCATATATAATATGTTCCACCTTTATTGTAAAACTGAAGTTCATCAGAGGGCTCTTAATTGGAACCAGTCAACTGTAATCTCACTTTGTTCCACCTTTATTGGACCAATTCCTATGGTTATTTGCAGAATTTGAGAGTGTACATAAAGCTTCCAATCTATCTCGGCATGTCTTTTTTCTGCAAGAACACAGCTAAGAATCTTTATTGCTGGGTTTGTTTAGTGTGAAAATACCACACATTGTCCTTTGAACTTCCCTGTGGCTCGCCAATGTGACTTATTTATTTGAGCTATCTTAGCAGAACCTTCTGTGGGCTCATTATCTTACTACCCTTTGGCGTTCCATACCTACGATTCGACCCATGGAAAGTAGAGATTATGAATATGCAAGTTATAATGATTGAAGGGCAGTATTGATTTCTTTTTCTGTAACAGGTTTGGAAGAGCGTAAAAGATTTGTGCAAACATATATGGGTGCTAAAGGTAATTTTACAAGATACTATAGAATAGGACCTATGAACGGTCGTCAAGGGATCAAATAATATGCAGCCATGCCAGTTCGTGTCTGTATGAATACAATATAATGCTCATTCCTAAGATGCTTTGATTTTCTGTGCAAGTTCTTTTCTTCTGCCCTTTTTGTTTAATGCCCTCCCTGTCCTAAATTTCAAGGATCCTTCAAATTTCTAATGTCTACCATTCAAGAAGTTCAAAATTTTGGGTGATGAAGATAGATTTTCTGGAAGTTCAAAGATGCATTTGTATGGCATTATATTGTTTCCTTGAGCTACTTATATTGATTTTTGAGCTCTCATGTTATGTTACACATATGCAGGTCAAGCAGCCCAGTGATGCTGAGGTGGAGAAATTAGTCCAAGATGTTGAGAAGTATACTCTTGCTAGCCATCTTGTTTTTGGGGTCTCTCGGGAATAATATTGGTAATTTCGCTCCTCTAACACCGAACCATTTATTTTTTTCAATAAGTTATAGGCATTTTTTTGGTTAATTATGTGTGCTAACTGCGAAAAGAAATCAGATAGTATTGTTTGTGTTTTATGCTTCGAATTGTGATATCGTAAGTCTTAACTTAATCTCCTAAGAACGTCCTTAATCATCGATTCGTTCATTTCATTTTCAGGAGCATGTAACTTAAGCCTGATTTGTCGTTGTTGCTTCCCAGTAATAAATGCATTTGTTACAAGCAGCAGAGAGGCAACAAATAGTAAAACAGTTATTAAGATGGAAAATGAGATGATCTATGTCCAGCATAATATATTTACCACACCAAAGGTTTGAATATTTTCTTTATTTTCAAATTGCTTTGTGATCTGCAGCAGAAATACCCTCGTTGCATTTTATACTAACTCCTTTTAAATTATATTTAAATTATATTATAATAAAGTTAGTGGACCAAGAGCATTACAACCATCAATCATACAAAAATTTATCTCAATAATTATTACTGCTACTTTGGATCTTGTTCTTAATGTCTAATTATTAAGAACAATGTCCACAATAATTTCTTTGGTATTGTACAAAATGTTTCTGATTTATGCGTGATATTTATTGCTGTAGTGGGTGGGGTATGCTTTGTCATGCGATACTCCAGAAGGTGAGGCTTGTGAACTTGTGAAAAACTTGGCTCTGATGACTCGTGTAACGACAAAGGCCCATTAATTTCTATGGTATTGCTCACAACCGTTACTTCTTTAATCGCATTTGGGCCCTCTTTTTTTCATAGCATATATTCTCCCTTGTGTGAACTGCTGAATCATGCTTGACAATAGGGTTGAATAGAAGAGTCAGGTTATTCCCATAATTATCTTCTTTAGGAACAATTGCTCGAGATGAAAGAAGATGTTGGTTGTTATCACTCAGTTGACACGAAACAATTGTCAAATAAAAAAGAATTGTTCAAGATCTTGTTGGAGTTAAATAACAATTGTTCGAGATGGAAGAGATTCAATATGAAGGATGGATGATGACATGTACCGGTAAGAAGTTACCCATTTCAGAGTTGATGAGTAGATCTCATTTCCCTAGTTTTTATATTTTCATTGTTTATAACGAGTTTTTACTGTCCATGAGCTTACCTTCCAAAAGCCTTAACATGCTGATGTTTGTTCAAGCTCGAGTTACTGTTGACTACATGCTCACAACTTCTTTATTCGACACTTAAGAATTTCATTAACATGGTTAAGTTAAGGGCATGACTTTAATATCATGTTAGGAATCACGACTCTCCATAATGGTATGATAGTCCACTTTGAATATAAGCTCTCATGCTTTTATTTTTTGTTTCCCCAAAAGTCCTCGTACCAATGGAGATATATTCATTACTTATAAATTCATGATCATCTCCTTAATTAGCCAACGTGGGACCCTTCCAACCATCTTCAACCATTATAGATCTAAAGCTAATAATGGGAGCTGAATAGTTTTACAAAGACCCCATCAGGATTCTTAGCAAAGCCTTAGCGGATTGCTCTGTTCTTAGAGAACTAAAGGTATGAGAAGAAGAAATTTGATATTGTAAAGAGAGTGAATCACCATAATACAACCCCAGAGCCAATGCTGATAGAAATTAGTATATCCACCATCGAGTATGAGGATAATAAGCATCTCCGCCAACATCCCATCAAGCACATCTTCAACTTGTAAAAAATCTGTTAGTGGTGGGCTTGGGCCGTTACAAATGGTATTAGAGCCAGATAAGGGGTATGTACCAGCGAGGAGGCTGAGCCAGCCTCGAAAGGGCAGATGGGACCCAAAGTGGTGTGCCAACAAGGATGCTGGGCCCCGAAAAGGGGCAGATTGGGGGGTCCCACATCAATTGGAGAAGGAAATGAGTGTCAGCAAGAACGCTGGACCCCGAAGGGGTTGAATTGGGAAAGGAGCCATTTTGTCTATTGAATTCGTTTAAGTGTCGGCTGGCCCAGTAATCAGAACCATTTATTACTTGAAGCTTACCATAGGAGAGCCTCTAATTCTTTAACTTCTAAAATGCATGAAGATCTTTCGTACATAAATTAGACAAATATTGATTTCTCTGGATTTCGTGTGTTCATGTCAACATATTTCTTGCAACTTTTTCCTTCTGCAAGTTTAAAAGAATATTAATCTTATTTACAAGAAAGGCTAAAGGTGTTCTATTTGAAGATGATGGCTATGGATACGGTATCAGTTTAGGTGCATACCTAACCTATCAACATATTATGTGGCAGGGCTAGAATCTTCAGTTCAAGCTTCTAGGACTGAAGGATCATGAGCTAGACCAAGACGGTGTCTGCATAGGCAAAGGTACGTTTTTGTTTGCATTTTCTTTTATTTTCGAAGCTCTCTACTGCATATTTTGAGTATCTATACCTCAAGATTCATACTTGGGGCACAGATGAAGAAGGTATTGCTTCTCCTTGCTTGGTCTCGTCTGACTCATGTTTCAACGAATAAAAACAAAAATGAAATATACAAACTTTAATTAATAAATGAAACCAAGGATATACAAAACTTATCTAAATAGTCACGACGGTCTGCCTGCCAGCGAGTGCTAGCGAGGATGTTGGGCCTTGAAAGGGGGTGTATTGTGAGATC

mRNA sequence

ATGACTAAAGACGTGGTGATTGGGAAGATGCCTATTGGCGAGCCTTTTGTGACCGTGAATGCTGTCAGTGAATCTATTAGTTTTCATATGTGCCGCTTATCTGACCTTACGTTCTTTGATGTAAGAATTAGCAAGCCTTGTCCTCATATGTGCCACTTATCTGACCTTATGTATGGTTCGTTCGTGAATGTTATTACCATTTACCATAAAAATTTGAGAGTGTACATAAAGCTTCCAATCTATCTCGGCATGTCTTTTTTCTGCAAGAACACAGCTAAGAATCTTTATTGCTGGGTTTGGAAGAGCGTAAAAGATTTGTGCAAACATATATGGGTGCTAAAGGTCAAGCAGCCCAGTGATGCTGAGGTGGAGAAATTAGTCCAAGATGTTGAGAAGTATACTCTTGCTAGCCATCTTGTTTTTGGGTGGGTGGGGTATGCTTTGTCATGCGATACTCCAGAAGGTGAGGCTTGTGAACTTGTGAAAAACTTGGCTCTGATGACTCGTGTAACGACAAAGGCCCATTAATTTCTATGGTATTGCTCACAACCGTTACTTCTTTAATCGCATTTGGGCCCTCTTTTTTTCATAGCATATATTCTCCCTTGTGTGAACTGCTGAATCATGCTTGACAATAGGGTTGAATAGAAGAGTCAGGTTATTCCCATAATTATCTTCTTTAGGAACAATTGCTCGAGATGAAAGAAGATGTTGGTTGTTATCACTCAGTTGACACGAAACAATTGTCAAATAAAAAAGAATTGTTCAAGATCTTGTTGGAGTTAAATAACAATTGTTCGAGATGGAAGAGATTCAATATGAAGGATGGATGATGACATGTACCGGGCTAGAATCTTCAGTTCAAGCTTCTAGGACTGAAGGATCATGAGCTAGACCAAGACGGTGTCTGCATAGGCAAAGCGAGTGCTAGCGAGGATGTTGGGCCTTGAAAGGGGGTGTATTGTGAGATC

Coding sequence (CDS)

ATGACTAAAGACGTGGTGATTGGGAAGATGCCTATTGGCGAGCCTTTTGTGACCGTGAATGCTGTCAGTGAATCTATTAGTTTTCATATGTGCCGCTTATCTGACCTTACGTTCTTTGATGTAAGAATTAGCAAGCCTTGTCCTCATATGTGCCACTTATCTGACCTTATGTATGGTTCGTTCGTGAATGTTATTACCATTTACCATAAAAATTTGAGAGTGTACATAAAGCTTCCAATCTATCTCGGCATGTCTTTTTTCTGCAAGAACACAGCTAAGAATCTTTATTGCTGGGTTTGGAAGAGCGTAAAAGATTTGTGCAAACATATATGGGTGCTAAAGGTCAAGCAGCCCAGTGATGCTGAGGTGGAGAAATTAGTCCAAGATGTTGAGAAGTATACTCTTGCTAGCCATCTTGTTTTTGGGTGGGTGGGGTATGCTTTGTCATGCGATACTCCAGAAGGTGAGGCTTGTGAACTTGTGAAAAACTTGGCTCTGATGACTCGTGTAACGACAAAGGCCCATTAA

Protein sequence

MTKDVVIGKMPIGEPFVTVNAVSESISFHMCRLSDLTFFDVRISKPCPHMCHLSDLMYGSFVNVITIYHKNLRVYIKLPIYLGMSFFCKNTAKNLYCWVWKSVKDLCKHIWVLKVKQPSDAEVEKLVQDVEKYTLASHLVFGWVGYALSCDTPEGEACELVKNLALMTRVTTKAH
BLAST of Carg16281 vs. NCBI nr
Match: XP_023540536.1 (uncharacterized protein LOC111800873 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023540537.1 uncharacterized protein LOC111800873 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023540538.1 uncharacterized protein LOC111800873 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023540539.1 uncharacterized protein LOC111800873 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023540540.1 uncharacterized protein LOC111800873 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 195.7 bits (496), Expect = 1.3e-46
Identity = 113/175 (64.57%), Postives = 124/175 (70.86%), Query Frame = 0

Query: 10  MPIGEPFVTVNAVSESISFHMCRLSDLTFFDVRISKPCPXXXXXXXXMYGSFVNVITIYH 69
           MPIGEPFVTVNAVSESISFHMCRLSDLTFFDVRISKPCP         YGSFVNVITIYH
Sbjct: 1   MPIGEPFVTVNAVSESISFHMCRLSDLTFFDVRISKPCPHMRHLSDLTYGSFVNVITIYH 60

Query: 70  KNLRVYIKLPIYLGMSFFCKNTAKNLYCWVWKSVKDLCKHIWV---LKVKQPSDAEVEKL 129
           K     + +   L       +T K        S+++  + I+V   +  K PSD+EVEKL
Sbjct: 61  K----VVLIQEQLSKDRIIIDTDKR------GSLEE--RKIFVQTYMGAKGPSDSEVEKL 120

Query: 130 VQDVEKYTLASHLVFG------WVGYALSCDTPEGEACELVKNLALMTRVTTKAH 176
           VQDVEKYTLASHLV+G      WVGYALS DTPEGEACELVKNLALMT VTTKAH
Sbjct: 121 VQDVEKYTLASHLVWGLSGIISWVGYALSRDTPEGEACELVKNLALMTHVTTKAH 163

BLAST of Carg16281 vs. NCBI nr
Match: XP_023540543.1 (uncharacterized protein LOC111800873 isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 140.6 bits (353), Expect = 5.1e-30
Identity = 83/139 (59.71%), Postives = 94/139 (67.63%), Query Frame = 0

Query: 10  MPIGEPFVTVNAVSESISFHMCRLSDLTFFDVRISKPCPXXXXXXXXMYGSFVNVITIYH 69
           MPIGEPFVTVNAVSESISFHMCRLSDLTFFDVRISKPCP         YGSFVNVITIYH
Sbjct: 1   MPIGEPFVTVNAVSESISFHMCRLSDLTFFDVRISKPCPHMRHLSDLTYGSFVNVITIYH 60

Query: 70  KNLRVYIKLPIYLGMSFFCKNTAKNLYCWVWKSVKDLCKHIWV---LKVKQPSDAEVEKL 129
           K     + +   L       +T K        S+++  + I+V   +  K PSD+EVEKL
Sbjct: 61  K----VVLIQEQLSKDRIIIDTDKR------GSLEE--RKIFVQTYMGAKGPSDSEVEKL 120

Query: 130 VQDVEKYTLASHLVFGWVG 146
           VQDVEKYTLASHLV+G  G
Sbjct: 121 VQDVEKYTLASHLVWGLSG 127

BLAST of Carg16281 vs. NCBI nr
Match: XP_023540542.1 (uncharacterized protein LOC111800873 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 140.2 bits (352), Expect = 6.7e-30
Identity = 85/149 (57.05%), Postives = 97/149 (65.10%), Query Frame = 0

Query: 36  LTFFDVRISKPCPXXXXXXXXMYGSFVNVITIYHKNLRVYIKLPIYLGMSFFCKNTAKNL 95
           + FFDVRISKPCP         YGSFVNVITIYHK     + +   L       +T K  
Sbjct: 1   MLFFDVRISKPCPHMRHLSDLTYGSFVNVITIYHK----VVLIQEQLSKDRIIIDTDKR- 60

Query: 96  YCWVWKSVKDLCKHIWV---LKVKQPSDAEVEKLVQDVEKYTLASHLVFG------WVGY 155
                 S+++  + I+V   +  K PSD+EVEKLVQDVEKYTLASHLV+G      WVGY
Sbjct: 61  -----GSLEE--RKIFVQTYMGAKGPSDSEVEKLVQDVEKYTLASHLVWGLSGIISWVGY 120

Query: 156 ALSCDTPEGEACELVKNLALMTRVTTKAH 176
           ALS DTPEGEACELVKNLALMT VTTKAH
Sbjct: 121 ALSRDTPEGEACELVKNLALMTHVTTKAH 137

BLAST of Carg16281 vs. NCBI nr
Match: XP_023540541.1 (uncharacterized protein LOC111800873 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 140.2 bits (352), Expect = 6.7e-30
Identity = 85/149 (57.05%), Postives = 97/149 (65.10%), Query Frame = 0

Query: 36  LTFFDVRISKPCPXXXXXXXXMYGSFVNVITIYHKNLRVYIKLPIYLGMSFFCKNTAKNL 95
           + FFDVRISKPCP         YGSFVNVITIYHK     + +   L       +T K  
Sbjct: 7   IKFFDVRISKPCPHMRHLSDLTYGSFVNVITIYHK----VVLIQEQLSKDRIIIDTDKR- 66

Query: 96  YCWVWKSVKDLCKHIWV---LKVKQPSDAEVEKLVQDVEKYTLASHLVFG------WVGY 155
                 S+++  + I+V   +  K PSD+EVEKLVQDVEKYTLASHLV+G      WVGY
Sbjct: 67  -----GSLEE--RKIFVQTYMGAKGPSDSEVEKLVQDVEKYTLASHLVWGLSGIISWVGY 126

Query: 156 ALSCDTPEGEACELVKNLALMTRVTTKAH 176
           ALS DTPEGEACELVKNLALMT VTTKAH
Sbjct: 127 ALSRDTPEGEACELVKNLALMTHVTTKAH 143

BLAST of Carg16281 vs. TAIR10
Match: AT5G45140.1 (nuclear RNA polymerase C2)

HSP 1 Score: 46.6 bits (109), Expect = 1.8e-05
Identity = 21/28 (75.00%), Postives = 22/28 (78.57%), Query Frame = 0

Query: 145 GYALSCDTPEGEACELVKNLALMTRVTT 173
           G    CDTPEGE+C LVKNLALMT VTT
Sbjct: 488 GMLCPCDTPEGESCGLVKNLALMTHVTT 515

BLAST of Carg16281 vs. TAIR10
Match: AT1G74320.1 (Protein kinase superfamily protein)

HSP 1 Score: 44.3 bits (103), Expect = 9.1e-05
Identity = 19/30 (63.33%), Postives = 26/30 (86.67%), Query Frame = 0

Query: 116 KQPSDAEVEKLVQDVEKYTLASHLVFGWVG 146
           ++PSD  V+KL++DVEKYTLASHL++G  G
Sbjct: 283 EKPSDTMVKKLLEDVEKYTLASHLIWGLWG 312

BLAST of Carg16281 vs. Swiss-Prot
Match: sp|F4KD38|NRPC2_ARATH (DNA-directed RNA polymerase III subunit 2 OS=Arabidopsis thaliana OX=3702 GN=NRPC2 PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 3.3e-04
Identity = 21/28 (75.00%), Postives = 22/28 (78.57%), Query Frame = 0

Query: 145 GYALSCDTPEGEACELVKNLALMTRVTT 173
           G    CDTPEGE+C LVKNLALMT VTT
Sbjct: 488 GMLCPCDTPEGESCGLVKNLALMTHVTT 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023540536.11.3e-4664.57uncharacterized protein LOC111800873 isoform X1 [Cucurbita pepo subsp. pepo] >XP... [more]
XP_023540543.15.1e-3059.71uncharacterized protein LOC111800873 isoform X4 [Cucurbita pepo subsp. pepo][more]
XP_023540542.16.7e-3057.05uncharacterized protein LOC111800873 isoform X3 [Cucurbita pepo subsp. pepo][more]
XP_023540541.16.7e-3057.05uncharacterized protein LOC111800873 isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G45140.11.8e-0575.00nuclear RNA polymerase C2[more]
AT1G74320.19.1e-0563.33Protein kinase superfamily protein[more]
Match NameE-valueIdentityDescription
sp|F4KD38|NRPC2_ARATH3.3e-0475.00DNA-directed RNA polymerase III subunit 2 OS=Arabidopsis thaliana OX=3702 GN=NRP... [more]
Match NameE-valueIdentityDescription
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16281-RACarg16281-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSUPERFAMILYSSF64484beta and beta-prime subunits of DNA dependent RNA-polymerasecoord: 144..173