Carg15423 (gene) Silver-seed gourd

Overview
NameCarg15423
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionobtusifoliol 14-alpha demethylase
LocationCucurbita_argyrosperma_scaffold_069 : 71171 .. 72813 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCCGGATAACAAATTCTTCAATGTCGGTCTGCTCGTAGTGGCCACTTTAGTCCTTGCGAAGGTCATTTCTTCCGTTCTGAAATCCAGATCTAAGAAACGGGCTCCTCCCACTGTGAGAGGGTTGCCCTTGGTCGGGGGAATGGTCCGTTTTCTTAAGGGTCCGATTGTGATGCTGAGGGAGGAGTACCCTAAGCTTGGGAGTGTCTTCACCTTGAACCTATTGCACAAGAAAATCACCTTCTTGATTGGGCCTGAGGTTTCTGCACATTTCTTCAAGGCGTCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTGCCTACTTTTGGGCCTGGCGTTGTGTTTGATGTGGATTACTCCATTCGCCAAGAACAGTTCAGGTTCTTCACTGAGGCTCTCAGAGTCACTAAATTGAAGGGCTATGTGGATCAGATGGTCAAAGAAGCAGAGGTCAGTAATTCAGCTATAAAAATCTAGTTTTCAACTTTAACAACCATCATTAAATGTTGTTCTTCATAATCTATGGCTGAACTTCATTTCTAGTTCCTGATGCTTGAGTCTAAATCTTCAATTTCCATTGTTTTTCTGCAAAAACTGTTTGTAACAATGGAATGTGGTGGGGTATTGAGCAGGATTACTTCTCTAAGTGGGGAGACAGTGGGGAAGTGGACCTCAAGTATGAGTTGGAGCATCTTATCATCTTAACAGCTAGTAGATGTCTATTGGGTAGAGAGGTTCGTGATAAGCTTTTTGCGGACGTTTCTGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTAATGTTCCCCTACCTGCCTATCCCAGCTCATCGTCGTCGTGATCGGGCCCGCAGCAAGCTTGCGGATATCTTTGCTAAGATCATAGCTTCCAGAAAATGCAATGGAAGTTCAGAGAATGACATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGTCGACCAACGACAGATACTGAGGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACACACTAGTTCTATTACCTCTACTTGGACTGGGGCTTATCTTCTACGCCACAAAGAGTACATGTCTGCTGTTATAGAGGAGCAGCAAAAGCTGATGGAAAAACATGGAGATAAGACTGATCATGATGTCTTGTCTGAAATGGACAATCTCCATAGGTGTATTAAAGAAGCTCTCAGACTCCACCCTCCATTGATTATGTTGATGCGCAGTTCACATACTGATTTTAGTGTGACCACTAAAGAGGGGAAAGCATATGATATACCAAAGGGTCATATAATTGCTACATCTCCAGCTTTCGCTAATCGACTTCCTCATGTCTATAAGGATCCAGACAGGTACGATCCAGACAGGTTTTCCCCCGAACGAGATGAGGACAAGGCAGCTGGACCATTCTCCTACATCTCGTTTGGAGGCGGTAGGCACGGGTGTCTCGGCGAGCCCTTCGCTTATCTACAGATAAAGGCAATTTGGAGTAGGTTGTTAAGAAATTTTGAACTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAACGCCATGGTTGTGGGTGTCAAAGGGAAAGTTATGGTCAGGTACAAGAGGAGGAAGCTTTCAGTTAGATAA

mRNA sequence

ATGGAGCCGGATAACAAATTCTTCAATGTCGGTCTGCTCGTAGTGGCCACTTTAGTCCTTGCGAAGGTCATTTCTTCCGTTCTGAAATCCAGATCTAAGAAACGGGCTCCTCCCACTGTGAGAGGGTTGCCCTTGGTCGGGGGAATGGTCCGTTTTCTTAAGGGTCCGATTGTGATGCTGAGGGAGGAGTACCCTAAGCTTGGGAGTGTCTTCACCTTGAACCTATTGCACAAGAAAATCACCTTCTTGATTGGGCCTGAGGTTTCTGCACATTTCTTCAAGGCGTCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTGCCTACTTTTGGGCCTGGCGTTGTGTTTGATGTGGATTACTCCATTCGCCAAGAACAGTTCAGGTTCTTCACTGAGGCTCTCAGAGTCACTAAATTGAAGGGCTATGTGGATCAGATGGTCAAAGAAGCAGAGGATTACTTCTCTAAGTGGGGAGACAGTGGGGAAGTGGACCTCAAGTATGAGTTGGAGCATCTTATCATCTTAACAGCTAGTAGATGTCTATTGGGTAGAGAGGTTCGTGATAAGCTTTTTGCGGACGTTTCTGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTAATGTTCCCCTACCTGCCTATCCCAGCTCATCGTCGTCGTGATCGGGCCCGCAGCAAGCTTGCGGATATCTTTGCTAAGATCATAGCTTCCAGAAAATGCAATGGAAGTTCAGAGAATGACATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGTCGACCAACGACAGATACTGAGGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACACACTAGTTCTATTACCTCTACTTGGACTGGGGCTTATCTTCTACGCCACAAAGAGTACATGTCTGCTGTTATAGAGGAGCAGCAAAAGCTGATGGAAAAACATGGAGATAAGACTGATCATGATGTCTTGTCTGAAATGGACAATCTCCATAGGTGTATTAAAGAAGCTCTCAGACTCCACCCTCCATTGATTATGTTGATGCGCAGTTCACATACTGATTTTAGTGTGACCACTAAAGAGGGGAAAGCATATGATATACCAAAGGGTCATATAATTGCTACATCTCCAGCTTTCGCTAATCGACTTCCTCATGTCTATAAGGATCCAGACAGGTACGATCCAGACAGGTTTTCCCCCGAACGAGATGAGGACAAGGCAGCTGGACCATTCTCCTACATCTCGTTTGGAGGCGGTAGGCACGGGTGTCTCGGCGAGCCCTTCGCTTATCTACAGATAAAGGCAATTTGGAGTAGGTTGTTAAGAAATTTTGAACTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAACGCCATGGTTGTGGGTGTCAAAGGGAAAGTTATGGTCAGGTACAAGAGGAGGAAGCTTTCAGTTAGATAA

Coding sequence (CDS)

ATGGAGCCGGATAACAAATTCTTCAATGTCGGTCTGCTCGTAGTGGCCACTTTAGTCCTTGCGAAGGTCATTTCTTCCGTTCTGAAATCCAGATCTAAGAAACGGGCTCCTCCCACTGTGAGAGGGTTGCCCTTGGTCGGGGGAATGGTCCGTTTTCTTAAGGGTCCGATTGTGATGCTGAGGGAGGAGTACCCTAAGCTTGGGAGTGTCTTCACCTTGAACCTATTGCACAAGAAAATCACCTTCTTGATTGGGCCTGAGGTTTCTGCACATTTCTTCAAGGCGTCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTGCCTACTTTTGGGCCTGGCGTTGTGTTTGATGTGGATTACTCCATTCGCCAAGAACAGTTCAGGTTCTTCACTGAGGCTCTCAGAGTCACTAAATTGAAGGGCTATGTGGATCAGATGGTCAAAGAAGCAGAGGATTACTTCTCTAAGTGGGGAGACAGTGGGGAAGTGGACCTCAAGTATGAGTTGGAGCATCTTATCATCTTAACAGCTAGTAGATGTCTATTGGGTAGAGAGGTTCGTGATAAGCTTTTTGCGGACGTTTCTGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTAATGTTCCCCTACCTGCCTATCCCAGCTCATCGTCGTCGTGATCGGGCCCGCAGCAAGCTTGCGGATATCTTTGCTAAGATCATAGCTTCCAGAAAATGCAATGGAAGTTCAGAGAATGACATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGTCGACCAACGACAGATACTGAGGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACACACTAGTTCTATTACCTCTACTTGGACTGGGGCTTATCTTCTACGCCACAAAGAGTACATGTCTGCTGTTATAGAGGAGCAGCAAAAGCTGATGGAAAAACATGGAGATAAGACTGATCATGATGTCTTGTCTGAAATGGACAATCTCCATAGGTGTATTAAAGAAGCTCTCAGACTCCACCCTCCATTGATTATGTTGATGCGCAGTTCACATACTGATTTTAGTGTGACCACTAAAGAGGGGAAAGCATATGATATACCAAAGGGTCATATAATTGCTACATCTCCAGCTTTCGCTAATCGACTTCCTCATGTCTATAAGGATCCAGACAGGTACGATCCAGACAGGTTTTCCCCCGAACGAGATGAGGACAAGGCAGCTGGACCATTCTCCTACATCTCGTTTGGAGGCGGTAGGCACGGGTGTCTCGGCGAGCCCTTCGCTTATCTACAGATAAAGGCAATTTGGAGTAGGTTGTTAAGAAATTTTGAACTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAACGCCATGGTTGTGGGTGTCAAAGGGAAAGTTATGGTCAGGTACAAGAGGAGGAAGCTTTCAGTTAGATAA

Protein sequence

MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKIIASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFSPERDEDKAAGPFSYISFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRKLSVR
Homology
[Close]
BLAST of Carg15423 vs. NCBI nr
Match: XP_022932660.1 (sterol 14-demethylase-like [Cucurbita moschata] >XP_022932661.1 sterol 14-demethylase-like [Cucurbita moschata] >XP_022932662.1 sterol 14-demethylase-like [Cucurbita moschata])

HSP 1 Score: 954.5 bits (2466), Expect = 1.4e-274
Identity = 486/486 (100.00%), Postives = 486/486 (100.00%), Query Frame = 0 

Query: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML 60
           MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML
Sbjct: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML 60

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180
           VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240
           SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI
Sbjct: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYI 420
           FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYI
Sbjct: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSVR 487
           RKLSVR
Sbjct: 481 RKLSVR 486

[Close]
BLAST of Carg15423 vs. NCBI nr
Match: XP_023539138.1 (sterol 14-demethylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 952.2 bits (2460), Expect = 6.8e-274
Identity = 484/486 (99.59%), Postives = 485/486 (99.79%), Query Frame = 0 

Query: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML 60
           MEPDNKFFNVGLLVVATLVLAKVISSVLKSRS KRAPPTVRGLPLVGGMVRFLKGPIVML
Sbjct: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSNKRAPPTVRGLPLVGGMVRFLKGPIVML 60

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180
           VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240
           SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI
Sbjct: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAVIEEQQKLMEKHGDKTDHD+LSEMDNLHRCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYI 420
           FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYI
Sbjct: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSVR 487
           RKLSVR
Sbjct: 481 RKLSVR 486

[Close]
BLAST of Carg15423 vs. NCBI nr
Match: XP_022972137.1 (sterol 14-demethylase-like [Cucurbita maxima] >XP_022972138.1 sterol 14-demethylase-like [Cucurbita maxima])

HSP 1 Score: 943.3 bits (2437), Expect = 3.2e-271
Identity = 480/486 (98.77%), Postives = 483/486 (99.38%), Query Frame = 0 

Query: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML 60
           MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGM+RFLKGPIVML
Sbjct: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMLRFLKGPIVML 60

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           REEYPKLGSVF LNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  REEYPKLGSVFILNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180
           VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240
           SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI
Sbjct: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAVIEEQ+KLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHT+
Sbjct: 301 LRHKEYMSAVIEEQKKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTN 360

Query: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYI 420
           FSV TKEGK YDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYI
Sbjct: 361 FSVITKEGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSVR 487
           RKLSVR
Sbjct: 481 RKLSVR 486

[Close]
BLAST of Carg15423 vs. NCBI nr
Match: XP_022980879.1 (sterol 14-demethylase-like [Cucurbita maxima])

HSP 1 Score: 910.6 bits (2352), Expect = 2.3e-261
Identity = 457/485 (94.23%), Postives = 472/485 (97.32%), Query Frame = 0 

Query: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML 60
           MEPDNKF NVGLLVVATL+LAKVIS+VL SRS KRAPPTV+G PLVGG+VRFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           R+EYPKLG+VFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180
           VDYSIRQEQFRFFTEALRVTKLKGYVDQMV EAED+FSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVSEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240
           SRCLLG EVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRK +GSSENDMLQCFIDSKYKDGRPTTD+EVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAV+EEQQ+LM KHGDK DHD+LSEMDNL+RCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYI 420
           FSVTT+EGK YDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXX   EDKAAGPFSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXGRDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWS+LLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSV 486
           RKLSV
Sbjct: 481 RKLSV 485

[Close]
BLAST of Carg15423 vs. NCBI nr
Match: XP_023527569.1 (sterol 14-demethylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 910.2 bits (2351), Expect = 3.0e-261
Identity = 457/485 (94.23%), Postives = 472/485 (97.32%), Query Frame = 0 

Query: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML 60
           MEPDNKF NVGLLVVATL+LAKVIS+VL SRS KRAPPTV+G PLVGG+VRFLKGPIVML
Sbjct: 1   MEPDNKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVML 60

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           R+EYPKLG+VFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 61  RKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120

Query: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180
           VDYSIRQEQFRFFTEALRVTKLKGYVDQMV EAED+FSKWGDSGEVDLKYELEHLIILTA
Sbjct: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILTA 180

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240
           SRCLLG EVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI
Sbjct: 181 SRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRK +GSSENDMLQCFIDSKYKDGRPTTD+EVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 241 IASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAYL 300

Query: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEYMSAV+EEQQ+LM KHGDK DHD+LSEMDNL+RCIKEALRLHPPLIMLMRSSHTD
Sbjct: 301 LRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHTD 360

Query: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYI 420
           FSVTT+EGK YDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXX   EDKAAGPFSYI
Sbjct: 361 FSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXGRDEDKAAGPFSYI 420

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWS+LLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 421 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480

Query: 481 RKLSV 486
           RKLSV
Sbjct: 481 RKLSV 485

[Close]
BLAST of Carg15423 vs. TAIR10
Match: AT1G11680.1 (CYTOCHROME P450 51G1)

HSP 1 Score: 805.8 bits (2080), Expect = 1.4e-233
Identity = 392/486 (80.66%), Postives = 437/486 (89.92%), Query Frame = 0 

Query: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRS-KKRAPPTVRGL-PLVGGMVRFLKGPIV 60
           ++ +NK    GL++VATLV+AK+I S   S S KKR PPT++   PLVG +++FLKGPI+
Sbjct: 3   LDSENKLLKTGLVIVATLVIAKLIFSFFTSDSKKKRLPPTLKAWPPLVGSLIKFLKGPII 62

Query: 61  MLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 120
           MLREEYPKLGSVFT+NL+HKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV
Sbjct: 63  MLREEYPKLGSVFTVNLVHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 122

Query: 121 FDVDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIIL 180
           FDVDYS+RQEQFRFFTEALRV KLKGYVD MV EAEDYFSKWG+SGEVD+K ELE LIIL
Sbjct: 123 FDVDYSVRQEQFRFFTEALRVNKLKGYVDMMVTEAEDYFSKWGESGEVDIKVELERLIIL 182

Query: 181 TASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFA 240
           TASRCLLGREVRD+LF DVSALFHDLDNGMLPISV+FPYLPIPAHRRRDRAR KL++IFA
Sbjct: 183 TASRCLLGREVRDQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDRAREKLSEIFA 242

Query: 241 KIIASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGA 300
           KII SRK +G +ENDMLQCFI+SKYKDGR TT++EVTGLLIAALFAGQHTSSITSTWTGA
Sbjct: 243 KIIGSRKRSGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWTGA 302

Query: 301 YLLRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSH 360
           YL+R+KEY SA ++EQ+ L+ KHGDK DHD+LSEMD L+RCIKEALRLHPPLIMLMR+SH
Sbjct: 303 YLMRYKEYFSAALDEQKNLIAKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRASH 362

Query: 361 TDFSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFS 420
           +DFSVT ++GK YDIPKGHI+ATSPAFANRLPH++KDPD Y          EDKAAG FS
Sbjct: 363 SDFSVTARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFS 422

Query: 421 YISFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRY 480
           YI+FGGGRHGCLGEPFAYLQIKAIWS LLRNFELEL+SPFPEIDWNAMVVGVKG VMVRY
Sbjct: 423 YIAFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRY 482

Query: 481 KRRKLS 485
           KRR+LS
Sbjct: 483 KRRQLS 488

[Close]
BLAST of Carg15423 vs. TAIR10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3)

HSP 1 Score: 114.4 bits (285), Expect = 2.0e-25
Identity = 112/465 (24.09%), Postives = 195/465 (41.94%), Query Frame = 0 

Query: 18  LVLAKVISSVLKSRSKK-RAPPTVRGLPLVGGMVR-FLKGPIVMLREEYPKLGSVFTLNL 77
           L L + I+ V +S S K   PP   G P VG   + + + P V    +  + GSVF  ++
Sbjct: 17  LCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVFKTHV 76

Query: 78  LHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTE 137
           L      +  PE +A F   ++S L +           G   +F        +  +    
Sbjct: 77  LGCPCVMISSPE-AAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLR 136

Query: 138 ALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLF- 197
           A     ++  V  +   A++  + W D  +++   E++      A   +LG   +D+++ 
Sbjct: 137 AFMPDAIRNMVPHIESIAQESLNSW-DGTQLNTYQEMKTYTFNVALISILG---KDEVYY 196

Query: 198 -ADVSALFHDLDNG--MLPISVMFPYLPIPAHRRRDRARSKLADIFAKIIASRKCNGSSE 257
             D+   ++ L+ G   +PI+     LP     +  +AR +LA I A I++ R+ N SS 
Sbjct: 197 REDLKRCYYILEKGYNSMPIN-----LPGTLFHKAMKARKELAQILANILSKRRQNPSSH 256

Query: 258 NDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVI 317
            D+L  F++ K       TD ++   +I  +FA + T++   TW   YL  +   + AV 
Sbjct: 257 TDLLGSFMEDK----AGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVT 316

Query: 318 EEQQKLM--EKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTKEGK 377
           EEQ  +   +K G+    +   +M   +R I+E LR    L    R +  D      E +
Sbjct: 317 EEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV-----EYE 376

Query: 378 AYDIPKGHIIATSPAFAN--RLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYISFGGGRH 437
            Y IPKG  +   P F N      ++ DP ++           + A  P +++ FG G H
Sbjct: 377 GYLIPKGWKVL--PLFRNIHHNADIFSDPGKFDPSRF------EVAPKPNTFMPFGSGIH 436

Query: 438 GCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKG 473
            C G   A L+I  +   L   +   ++ P   I +    +   G
Sbjct: 437 SCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNG 454

[Close]
BLAST of Carg15423 vs. TAIR10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1)

HSP 1 Score: 104.4 bits (259), Expect = 2.1e-22
Identity = 99/423 (23.40%), Postives = 175/423 (41.37%), Query Frame = 0 

Query: 32  SKKRAPPTVRGLPLVGGMVR-FLKGPIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSA 91
           SK   PP   G P VG   + + + P V  + +  + GSVF  ++L      +  PE +A
Sbjct: 32  SKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKRYGSVFKTHVLGCPCVMISSPE-AA 91

Query: 92  HFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTEALRVTKLKGYVDQMV 151
            F   ++S L +           G   +F        +  +    A     ++  V  + 
Sbjct: 92  KFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIE 151

Query: 152 KEAEDYFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLF-ADVSALFHDLDNGML 211
             A+D    W +   ++   E++      A   + G++  + L+  D+   ++ L+ G  
Sbjct: 152 SIAQDSLRSW-EGTMINTYQEMKTYTFNVALLSIFGKD--EVLYREDLKRCYYILEKGYN 211

Query: 212 PISVMFPYLPIPAHRRRDRARSKLADIFAKIIASRKCNGSSENDMLQCFIDSKYKDGRPT 271
            + V    LP     +  +AR +L+ I A+I++ R+ NGSS ND+L  F+  K       
Sbjct: 212 SMPV---NLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFMGDK----EEL 271

Query: 272 TDTEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVIEEQQKLM--EKHGDKTDH 331
           TD ++   +I  +FA + T++   +W   YL  +   + AV EEQ  +   ++ G+    
Sbjct: 272 TDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTW 331

Query: 332 DVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTKEGKAYDIPKGHIIATSPAFAN 391
               +M    R I+E LR+   L    R +  D      E + Y IPKG  +   P F N
Sbjct: 332 GDTKKMPLTSRVIQETLRVASILSFTFREAVEDV-----EYEGYLIPKGWKVL--PLFRN 391

Query: 392 RLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYISFGGGRHGCLGEPFAYLQIKAIWSRLL 451
               ++   D +           + A  P +++ FG G H C G   A L++  +   L 
Sbjct: 392 ----IHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLT 432

[Close]
BLAST of Carg15423 vs. TAIR10
Match: AT3G61040.1 (cytochrome P450, family 76, subfamily C, polypeptide 7)

HSP 1 Score: 89.4 bits (220), Expect = 6.8e-18
Identity = 78/272 (28.68%), Postives = 120/272 (44.12%), Query Frame = 0 

Query: 196 DVSALFHDLDN---------GMLPISVMFPYLP-IPAHRRRDRAR---SKLADIFAKIIA 255
           D S  FHD  N         G   ++  FP+L  +     R  AR    KL  +F   I 
Sbjct: 197 DDSKTFHDFQNVVIRMMEISGKPNLADFFPFLGFLDLQGARKEARLLMHKLFRVFQGFID 256

Query: 256 SRKCNGS-SENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYLL 315
           +++ + S + NDML   +D  +K      D  +  LL+    AG  TSS    W  A LL
Sbjct: 257 TKRSSTSRNNNDMLDSLLDIAHKKESELDDNNIKHLLLDLFLAGVDTSSSAVEWAMAELL 316

Query: 316 RHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTDF 375
           R+ + +  V EE ++++   G   D D++ ++  L   +KE+LRLHPP   L+       
Sbjct: 317 RNPKMIVKVQEEIRQVIGLKGTVQDLDIV-KLPYLQAVVKESLRLHPPAPFLVPRKSESD 376

Query: 376 SVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYIS 435
            V   E   + IPK   +  +     R P+V+K+P ++          + K    F  I 
Sbjct: 377 DVQIFE---FLIPKNTQVLVNVWAIGRDPNVWKNPTQFEPERFLGRGIDVK-GNHFELIP 436

Query: 436 FGGGRHGCLGEPFAYLQIKAIWSRLLRNFELE 454
           FG GR  C G P A+  +  + + LL  F+ E
Sbjct: 437 FGAGRRICPGMPLAFRIMHLVLASLLYGFDWE 463

[Close]
BLAST of Carg15423 vs. TAIR10
Match: AT2G24180.1 (cytochrome p450 71b6)

HSP 1 Score: 81.3 bits (199), Expect = 1.9e-15
Identity = 79/270 (29.26%), Postives = 106/270 (39.26%), Query Frame = 0 

Query: 201 FHDLDNGMLPISVMFPYLPIPAHRRRDRA--RSKLADIFAKIIASRKCNGSSENDMLQCF 260
           F DLD         F    I  HR + +   R  L D+  K+ +     GSS        
Sbjct: 247 FQDLDT--------FYQKAIVDHREKKKTEDREDLIDVLLKLQSQETKLGSSR------- 306

Query: 261 IDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVIEEQQKLM 320
                      TDT +  +++    AG  TS IT  WT A L RH   M  V   Q ++ 
Sbjct: 307 ----------ITDTHIRAIIMDLFVAGVDTSVITLDWTMAELSRHPRVMKKV---QAEIR 366

Query: 321 EKHGDK--TDHDVLSEMDNLHRCIKEALRLHPPL-IMLMRSSHTDFSVTTKEGKAYDIPK 380
           E  GDK    +D L  +  +   IKE  RLH P  I++ R + T+F +     K YDI  
Sbjct: 367 EHVGDKGIVTYDDLEALVYMKMVIKETWRLHAPSPILIPREAMTNFKI-----KGYDIYP 426

Query: 381 GHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYISFGGGRHGCLGEPFA 440
           G  I  +     R P V+KDPD +          E K    F  + FG GR GC   P  
Sbjct: 427 GTRIHVNAWAIGRNPDVWKDPDEFIPERFVDSNVETKGTS-FELLPFGSGRRGC---PAM 471

Query: 441 YLQIKAIWSRLLRNFELELISPFPEIDWNA 466
           Y+ +  +        E  L +     DW A
Sbjct: 487 YVGLSTV--------EYTLANLLYHFDWKA 471

[Close]
BLAST of Carg15423 vs. Swiss-Prot
Match: sp|Q9SAA9|CP511_ARATH (Sterol 14-demethylase OS=Arabidopsis thaliana OX=3702 GN=CYP51G1 PE=1 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 2.6e-232
Identity = 392/486 (80.66%), Postives = 437/486 (89.92%), Query Frame = 0 

Query: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRS-KKRAPPTVRGL-PLVGGMVRFLKGPIV 60
           ++ +NK    GL++VATLV+AK+I S   S S KKR PPT++   PLVG +++FLKGPI+
Sbjct: 3   LDSENKLLKTGLVIVATLVIAKLIFSFFTSDSKKKRLPPTLKAWPPLVGSLIKFLKGPII 62

Query: 61  MLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 120
           MLREEYPKLGSVFT+NL+HKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV
Sbjct: 63  MLREEYPKLGSVFTVNLVHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVV 122

Query: 121 FDVDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIIL 180
           FDVDYS+RQEQFRFFTEALRV KLKGYVD MV EAEDYFSKWG+SGEVD+K ELE LIIL
Sbjct: 123 FDVDYSVRQEQFRFFTEALRVNKLKGYVDMMVTEAEDYFSKWGESGEVDIKVELERLIIL 182

Query: 181 TASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFA 240
           TASRCLLGREVRD+LF DVSALFHDLDNGMLPISV+FPYLPIPAHRRRDRAR KL++IFA
Sbjct: 183 TASRCLLGREVRDQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDRAREKLSEIFA 242

Query: 241 KIIASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGA 300
           KII SRK +G +ENDMLQCFI+SKYKDGR TT++EVTGLLIAALFAGQHTSSITSTWTGA
Sbjct: 243 KIIGSRKRSGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWTGA 302

Query: 301 YLLRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSH 360
           YL+R+KEY SA ++EQ+ L+ KHGDK DHD+LSEMD L+RCIKEALRLHPPLIMLMR+SH
Sbjct: 303 YLMRYKEYFSAALDEQKNLIAKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRASH 362

Query: 361 TDFSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFS 420
           +DFSVT ++GK YDIPKGHI+ATSPAFANRLPH++KDPD Y          EDKAAG FS
Sbjct: 363 SDFSVTARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFS 422

Query: 421 YISFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRY 480
           YI+FGGGRHGCLGEPFAYLQIKAIWS LLRNFELEL+SPFPEIDWNAMVVGVKG VMVRY
Sbjct: 423 YIAFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRY 482

Query: 481 KRRKLS 485
           KRR+LS
Sbjct: 483 KRRQLS 488

[Close]
BLAST of Carg15423 vs. Swiss-Prot
Match: sp|P93846|CP51_SORBI (Obtusifoliol 14-alpha demethylase OS=Sorghum bicolor OX=4558 GN=CYP51 PE=1 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 5.2e-217
Identity = 360/484 (74.38%), Postives = 420/484 (86.78%), Query Frame = 0 

Query: 3   PDNKFFNVGL-LVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVMLR 62
           P  +    GL LVVAT++  K++ S      KKR PPT+ G P+VGG+V+F++GPI M+R
Sbjct: 7   PQQQRLMAGLALVVATVIFLKLLLSFRSGGGKKRLPPTIPGAPVVGGLVKFMRGPIPMIR 66

Query: 63  EEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDV 122
           E+Y  LGSVFT+ ++ ++ITFLIGPEVSAHFFK +E+++SQQEVY+FNVPTFGPGVVFDV
Sbjct: 67  EQYAALGSVFTVPIITRRITFLIGPEVSAHFFKGNEAEMSQQEVYRFNVPTFGPGVVFDV 126

Query: 123 DYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTAS 182
           DYS+RQEQFRFFTEALR  KL+ YVDQMV EAE+YFSKWG+SG VDLKYELEHLIILTAS
Sbjct: 127 DYSVRQEQFRFFTEALRANKLRSYVDQMVAEAEEYFSKWGESGTVDLKYELEHLIILTAS 186

Query: 183 RCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKII 242
           RCLLGREVR+KLF DVSALFHDLDNG+ PISV+FPYLPIPAH+RRD+AR++LA+IFA II
Sbjct: 187 RCLLGREVREKLFDDVSALFHDLDNGIQPISVLFPYLPIPAHKRRDKARARLAEIFATII 246

Query: 243 ASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYLL 302
            SRK +G SE DMLQCFIDSKYK+GRPTT+ EVTGLLIAALFAGQHTSSITSTWTGAY+L
Sbjct: 247 KSRKASGQSEEDMLQCFIDSKYKNGRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAYML 306

Query: 303 RHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTDF 362
           R K+Y +  +EEQ+ +M++HGDK DHD+L+EMD L+RCIKEALRLHPPLIML+R SH+DF
Sbjct: 307 RFKQYFAEAVEEQKDVMKRHGDKIDHDILAEMDVLYRCIKEALRLHPPLIMLLRQSHSDF 366

Query: 363 SVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYIS 422
           +VTTKEGK YDIPKGHI+ATSP+FANRLPH+YK+PD Y          EDKAAG FSYIS
Sbjct: 367 TVTTKEGKEYDIPKGHIVATSPSFANRLPHIYKNPDSYDPDRFGPGREEDKAAGAFSYIS 426

Query: 423 FGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRR 482
           FGGGRHGCLGEPFAYLQIKAIW+ LLRNFE EL+SPFPE DWNAMVVG+KG+VMV YKRR
Sbjct: 427 FGGGRHGCLGEPFAYLQIKAIWTHLLRNFEFELVSPFPENDWNAMVVGIKGEVMVNYKRR 486

Query: 483 KLSV 486
           KL V
Sbjct: 487 KLVV 490

[Close]
BLAST of Carg15423 vs. Swiss-Prot
Match: sp|P93596|CP51_WHEAT (Obtusifoliol 14-alpha demethylase (Fragment) OS=Triticum aestivum OX=4565 GN=CYP51 PE=2 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 1.5e-211
Identity = 347/451 (76.94%), Postives = 403/451 (89.36%), Query Frame = 0 

Query: 35  RAPPTVRGLPLVGGMVRFLKGPIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFK 94
           R PPT+ G P+VGG++RFL+GPI ++R EY +LG VFT+ +L ++ITFLIGP+VSAHFFK
Sbjct: 1   RPPPTIPGAPVVGGLLRFLRGPIPLIRAEYARLGPVFTVPILTRRITFLIGPDVSAHFFK 60

Query: 95  ASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAE 154
           ++ESD+SQQEVY+FNVPTFGPGVVFDVDY +RQEQFRFFTEALR  KL+ YVDQMV EAE
Sbjct: 61  SNESDMSQQEVYRFNVPTFGPGVVFDVDYQVRQEQFRFFTEALRANKLRSYVDQMVAEAE 120

Query: 155 DYFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFADVSALFHDLDNGMLPISVM 214
           +YFSKWG+SG VDLKYELEHLIILTASRCLLGREVR+KLF DVSALFHDLDNGMLPISV+
Sbjct: 121 EYFSKWGESGTVDLKYELEHLIILTASRCLLGREVREKLFDDVSALFHDLDNGMLPISVI 180

Query: 215 FPYLPIPAHRRRDRARSKLADIFAKIIASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEV 274
           FPYLPIPAHRRRD+AR++LA+IFA II SRK +G SE DMLQCFIDSKYK+GR TT++EV
Sbjct: 181 FPYLPIPAHRRRDQARTRLAEIFATIIKSRKASGQSEEDMLQCFIDSKYKNGRQTTESEV 240

Query: 275 TGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMD 334
           TGLLIAALFAGQHTSSITSTWTGAYLL+ ++Y +  +EEQ+++M++HGDK DHD+L+EMD
Sbjct: 241 TGLLIAALFAGQHTSSITSTWTGAYLLKFQQYFAEAVEEQKEVMKRHGDKIDHDILAEMD 300

Query: 335 NLHRCIKEALRLHPPLIMLMRSSHTDFSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYK 394
            L+RCIKEALRLHPPLIML+R SH+DFSVTT+EGK +DIPKGHI+ATSPAFANRLPH++K
Sbjct: 301 VLYRCIKEALRLHPPLIMLLRQSHSDFSVTTREGKEFDIPKGHIVATSPAFANRLPHIFK 360

Query: 395 DPDRYXXXXXXXXXXEDKAAGPFSYISFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELEL 454
           +PD Y          EDK AG FSYISFGGGRHGCLGEPFAYLQIKAIW+ LLRNFE EL
Sbjct: 361 NPDSYDPDRFAAGREEDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWTHLLRNFEFEL 420

Query: 455 ISPFPEIDWNAMVVGVKGKVMVRYKRRKLSV 486
           +SPFPE DWNAMVVG+KG+VMV YKRRKL V
Sbjct: 421 VSPFPENDWNAMVVGIKGEVMVNYKRRKLIV 451

[Close]
BLAST of Carg15423 vs. Swiss-Prot
Match: sp|Q1ZXH9|CP51_DICDI (Probable lanosterol 14-alpha demethylase OS=Dictyostelium discoideum OX=44689 GN=cyp51 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 3.9e-103
Identity = 205/473 (43.34%), Postives = 290/473 (61.31%), Query Frame = 0 

Query: 15  VATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVMLREEYPKLGSVFTLN 74
           +A LV+A ++    K +S    PP V  +P +G   +F K P+ ++R  Y +LG +FTL+
Sbjct: 5   IAVLVIAILVIFAFK-KSPSNIPPIVETIPFIGCFYQFAKNPLQLVRNSYDRLGEIFTLH 64

Query: 75  LLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFT 134
           L+  K+TF++GPE  A FF+ ++ +LS +E Y+F  P FG GVV+D +  I  EQ RF  
Sbjct: 65  LMGFKMTFVLGPEAQALFFRGTDEELSPKEAYRFVTPVFGKGVVYDSETEIMYEQLRFVK 124

Query: 135 EALRVTKLKGYVDQMVKEAEDYF-SKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKL 194
             L +++LK  V  + +E E YF +KWGDSGE+DL YE+  L ILTASRCL+G+ +   L
Sbjct: 125 NGLVLSQLKKAVGIIQEETEKYFETKWGDSGEIDLLYEMNKLTILTASRCLMGKSINKSL 184

Query: 195 --FADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKIIAS-RKCNGSS 254
                ++ L+H+L+ G+ PIS  FP LP+P+ ++RD AR+K+A IF  II   R+    S
Sbjct: 185 GQSGQLADLYHELEEGLNPISFFFPNLPLPSFKKRDAARAKVAAIFHSIIQERRRSTDDS 244

Query: 255 ENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAV 314
            +D+L   ++SKYKDG    D ++ GL+I  LFAGQHTSSIT T+T  YLL + EY    
Sbjct: 245 VDDVLYTLMNSKYKDGSVLEDEQIVGLMIGLLFAGQHTSSITLTYTIFYLLNNLEYFDET 304

Query: 315 IEEQQKLMEKHGD-KTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTKEGK 374
            ++   +++K    + + D L  M+ L   I+E LRLHPPLI LMR       +T  E K
Sbjct: 305 QKDINDIVQKENQGEINFDGLKRMNRLETVIREVLRLHPPLIFLMRK-----VMTPMEYK 364

Query: 375 AYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYISFGGGRHGC 434
              IP GHI+A SP    RLP VYK+PD +          EDK   PFS+I+FGGG+HGC
Sbjct: 365 GKTIPAGHILAVSPQVGMRLPTVYKNPDSF---EPKRFDVEDKT--PFSFIAFGGGKHGC 424

Query: 435 LGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRK 483
            GE F  LQIK IW+ L   + LE + P P  D+ ++V G KG  MV+Y +++
Sbjct: 425 PGENFGILQIKTIWTVLSTKYNLE-VGPVPPTDFTSLVAGPKGPCMVKYSKKQ 465

[Close]
BLAST of Carg15423 vs. Swiss-Prot
Match: sp|Q4PJW3|CP51A_BOVIN (Lanosterol 14-alpha demethylase OS=Bos taurus OX=9913 GN=CYP51A1 PE=2 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 4.1e-89
Identity = 184/458 (40.17%), Postives = 260/458 (56.77%), Query Frame = 0 

Query: 34  KRAPPTVRGLPLVGGMVRFLKGPIVMLREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFF 93
           K  P  V  +P +G  + F K PI  L + Y K G VF+  ++ K  T+L+G E +A  F
Sbjct: 58  KSPPYIVSPIPFLGHAIAFGKSPIEFLEDAYEKYGPVFSFTMVGKTFTYLLGSEAAALLF 117

Query: 94  KASESDLSQQEVY-QFNVPTFGPGVVFDVDYSIRQEQFRFFTEALRVTKLKGYVDQMVKE 153
            +   DL+ +EVY +   P FG GV +DV  ++  EQ +     L +   + +V  + KE
Sbjct: 118 NSKNEDLNAEEVYSRLTTPVFGKGVAYDVPNTVFLEQKKMLKSGLNIAHFRQHVSIIEKE 177

Query: 154 AEDYFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFADVSALFHDLDNGMLPIS 213
            ++YF  WG+SGE +L   L  LIILTAS CL G+E+R +L   V+ L+ DLD G    +
Sbjct: 178 TKEYFKSWGESGEKNLFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAA 237

Query: 214 VMFP-YLPIPAHRRRDRARSKLADIFAKIIASRKCNGSSENDMLQCFIDSKYKDGRPTTD 273
            + P +LP+P+ RRRDRA  ++ +IF K I  R+ +G   +D+LQ  ++S YKDGRP TD
Sbjct: 238 WLLPGWLPLPSFRRRDRAHREIKNIFYKAIQKRRESGEKIDDILQTLLESTYKDGRPLTD 297

Query: 274 TEVTGLLIAALFAGQHTSSITSTWTGAYLLRHKEYMSAVIEEQQKLMEKHGDKTDHDVLS 333
            EV G+LI  L AGQHTSS TS W G +L R K        EQ+ +  ++     +D L 
Sbjct: 298 DEVAGMLIGLLLAGQHTSSTTSAWMGFFLARDKTLQEKCFLEQKTVCGENLPPLTYDQLK 357

Query: 334 EMDNLHRCIKEALRLHPPLIMLMRSSHTDFSVTTKEGKAYDIPKGHIIATSPAFANRLPH 393
           +++ L RCIKE LRL PP++ +MR + T  +V       Y IP GH +  SP    RL  
Sbjct: 358 DLNLLDRCIKETLRLRPPIMTMMRLAKTPLTVA-----GYTIPPGHQVCVSPTVNQRLKD 417

Query: 394 VYKD-----PDRYXXXXXXXXXXEDKAAG--PFSYISFGGGRHGCLGEPFAYLQIKAIWS 453
            + +     PDRY          ED  A    F+Y+ FG GRH C+GE FAY+QIK IWS
Sbjct: 418 SWVERLDFNPDRY---------LEDSPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWS 477

Query: 454 RLLRNFELELISP-FPEIDWNAMVVGVKGKVMVRYKRR 482
            +LR +E +LI   FP +++  M +    K ++RYKRR
Sbjct: 478 TMLRLYEFDLIDGYFPTVNYTTM-IHTPEKPIIRYKRR 500

[Close]
BLAST of Carg15423 vs. TrEMBL
Match: tr|A0A1S3BXE7|A0A1S3BXE7_CUCME (sterol 14-demethylase OS=Cucumis melo OX=3656 GN=LOC103494635 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 1.3e-257
Identity = 448/486 (92.18%), Postives = 473/486 (97.33%), Query Frame = 0 

Query: 1   MEPD-NKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVM 60
           MEPD NKFFNVGLL++ATL+LAK+IS++L SRS KRAPPTV+G+P +GG++RFLKGPIVM
Sbjct: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LR+EYPKLGSVFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMV+EAED+FSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAK 240
           ASRCLLG EVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSS+NDMLQCFIDSKYKDGR TTD+EVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHT 360
           LL HKEYMSAV+EEQQ+LM KHGDK DHD+LSEMDNL+RCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSY 420
           DFSVTT+EGK YDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXX   EDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWS+LLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSV 486
           RRKLSV
Sbjct: 481 RRKLSV 486

[Close]
BLAST of Carg15423 vs. TrEMBL
Match: tr|A0A0A0KYP3|A0A0A0KYP3_CUCSA (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa4G007710 PE=2 SV=1)

HSP 1 Score: 895.6 bits (2313), Expect = 5.0e-257
Identity = 448/486 (92.18%), Postives = 471/486 (96.91%), Query Frame = 0 

Query: 1   MEPDN-KFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVM 60
           MEPDN KFFNVGLLV+ATL+LAK+IS++L SRS KR PPTV+G+P +GG++RFLKGPIVM
Sbjct: 1   MEPDNIKFFNVGLLVLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LREEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LR+EYPKLGSVFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMV+EAED+FSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAK 240
           ASRCLLG EVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSS+NDMLQCFIDSKYKDGR TTD+EVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHT 360
           LL HKEYMSAV+EEQQ+LM KHGDK DHD+LSEMDNLHRCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLCHKEYMSAVLEEQQELMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSY 420
           DFSVTT+EGK YDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXX   EDKAAG FSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXGRDEDKAAGAFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWS+LLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSV 486
           RRKLSV
Sbjct: 481 RRKLSV 486

[Close]
BLAST of Carg15423 vs. TrEMBL
Match: tr|A0A2I4FBQ0|A0A2I4FBQ0_9ROSI (sterol 14-demethylase OS=Juglans regia OX=51240 GN=LOC108997326 PE=3 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 1.8e-243
Identity = 410/485 (84.54%), Postives = 448/485 (92.37%), Query Frame = 0 

Query: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML 60
           ME DNK FN+GLL+VATLV+AK+IS+++  RS+KR PP V+  P++GG++RF+KGPI+ML
Sbjct: 4   MEVDNKLFNMGLLIVATLVVAKLISALIVPRSRKRLPPVVKSWPVIGGLIRFMKGPILML 63

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           REEYPKLGSVFT  L++KKITF IGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 64  REEYPKLGSVFTSTLVNKKITFFIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 123

Query: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180
           VDYS+RQEQFRFFTE+LRV KLKGYVDQMV EAEDYFSKWGDSGEVD+KYELEHLIILTA
Sbjct: 124 VDYSVRQEQFRFFTESLRVNKLKGYVDQMVAEAEDYFSKWGDSGEVDIKYELEHLIILTA 183

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240
           SRCLLGREVRDKLF DVSALFHDLDNGMLPISV+FPYLPIPAHRRRDRAR KLA+IFAKI
Sbjct: 184 SRCLLGREVRDKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDRARKKLAEIFAKI 243

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRKC G SENDMLQCFIDSKYKDGR TT+ E+TGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 244 IASRKCAGKSENDMLQCFIDSKYKDGRQTTEAEITGLLIAALFAGQHTSSITSTWTGAYL 303

Query: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           LRHKEY+SAV+EEQ+ LM KHG+K DHD+LSEMD L+RCIKEALRLHPPLI+L+RSSH+D
Sbjct: 304 LRHKEYLSAVLEEQKNLMGKHGNKVDHDILSEMDVLYRCIKEALRLHPPLILLLRSSHSD 363

Query: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYI 420
           FSVTT++GK YDIPKGHI+ATSPAFANRLPH+YKDPDRY          EDKAAG FSYI
Sbjct: 364 FSVTTRDGKEYDIPKGHIVATSPAFANRLPHIYKDPDRYDPDRFAIGREEDKAAGAFSYI 423

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWS LLRNFELEL+SPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 424 SFGGGRHGCLGEPFAYLQIKAIWSNLLRNFELELVSPFPEIDWNAMVVGVKGKVMVRYKR 483

Query: 481 RKLSV 486
           RKL V
Sbjct: 484 RKLCV 488

[Close]
BLAST of Carg15423 vs. TrEMBL
Match: tr|A0A2N9FAW8|A0A2N9FAW8_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS12205 PE=3 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 1.0e-241
Identity = 410/485 (84.54%), Postives = 448/485 (92.37%), Query Frame = 0 

Query: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML 60
           M+ DNKFFNVG+L+VATLV+AK+IS ++  RS KR PP V+  PL+GG+VRF+KGPIVML
Sbjct: 2   MDVDNKFFNVGILIVATLVVAKLISWLIMPRSSKRVPPVVKTWPLIGGLVRFMKGPIVML 61

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           REEYPKLGSVFTLNL +KKITFLIGPEVSAHFFKA ESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 62  REEYPKLGSVFTLNLANKKITFLIGPEVSAHFFKAPESDLSQQEVYQFNVPTFGPGVVFD 121

Query: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180
           VDYS+RQEQFRFFTE+LRV KLKGYVDQMV EAE+YFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 122 VDYSVRQEQFRFFTESLRVNKLKGYVDQMVTEAEEYFSKWGDSGEVDLKYELEHLIILTA 181

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240
           SRCLLGREVRDKLFADVSALFHDLDNGMLPISV+FPYLPIPAHRRRDRAR KL++IFA I
Sbjct: 182 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVIFPYLPIPAHRRRDRARKKLSEIFANI 241

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRK  G SENDMLQCFIDSKYKDGRPTT+ EVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 242 IASRKHAGKSENDMLQCFIDSKYKDGRPTTELEVTGLLIAALFAGQHTSSITSTWTGAYL 301

Query: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           L HK+Y+SAV+EEQ+ LM+KHG+K DHD+LSEMD L+R IKEALRLHPPLIML+RSSH+D
Sbjct: 302 LSHKKYLSAVLEEQKNLMQKHGNKVDHDILSEMDTLYRSIKEALRLHPPLIMLLRSSHSD 361

Query: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYI 420
           FSVTT++GK YDIPKGHI+ATSPAFANRLPH++++PDRY          EDKAAG FSYI
Sbjct: 362 FSVTTRDGKEYDIPKGHIVATSPAFANRLPHIFQEPDRYDPDRFAIGREEDKAAGAFSYI 421

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWS LLRNFELEL+SPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 422 SFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGKVMVRYKR 481

Query: 481 RKLSV 486
           R+LSV
Sbjct: 482 RELSV 486

[Close]
BLAST of Carg15423 vs. TrEMBL
Match: tr|A0A2P4JYN9|A0A2P4JYN9_QUESU (Sterol 14-demethylase OS=Quercus suber OX=58331 GN=CFP56_18230 PE=3 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 3.8e-241
Identity = 409/485 (84.33%), Postives = 446/485 (91.96%), Query Frame = 0 

Query: 1   MEPDNKFFNVGLLVVATLVLAKVISSVLKSRSKKRAPPTVRGLPLVGGMVRFLKGPIVML 60
           M+ DNKFFNVG+L+VATLV+AK+IS ++   SKKR PP V+  PL+GG+VRF+KGPIVM+
Sbjct: 4   MDVDNKFFNVGVLIVATLVVAKLISVLIMPNSKKRLPPVVKTWPLIGGLVRFMKGPIVMI 63

Query: 61  REEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 120
           REEYPKLGSVFTLNL +KKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD
Sbjct: 64  REEYPKLGSVFTLNLANKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFD 123

Query: 121 VDYSIRQEQFRFFTEALRVTKLKGYVDQMVKEAEDYFSKWGDSGEVDLKYELEHLIILTA 180
           VDYS+RQEQFRFFTE+LRV KLKGYVDQMV EAEDYFSKWGDSGEVDLKYELEHLIILTA
Sbjct: 124 VDYSVRQEQFRFFTESLRVNKLKGYVDQMVTEAEDYFSKWGDSGEVDLKYELEHLIILTA 183

Query: 181 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAKI 240
           SRCLLGREVRDKLFADVSALFHDLDNGMLPISV+FPYLPIPAH+RRD+AR KL++IFA I
Sbjct: 184 SRCLLGREVRDKLFADVSALFHDLDNGMLPISVIFPYLPIPAHKRRDQARKKLSEIFANI 243

Query: 241 IASRKCNGSSENDMLQCFIDSKYKDGRPTTDTEVTGLLIAALFAGQHTSSITSTWTGAYL 300
           IASRK  G SENDMLQCFIDSKYKDGRPTT+TEVTGLLIAALFAGQHTSSITSTWTGAYL
Sbjct: 244 IASRKDAGKSENDMLQCFIDSKYKDGRPTTETEVTGLLIAALFAGQHTSSITSTWTGAYL 303

Query: 301 LRHKEYMSAVIEEQQKLMEKHGDKTDHDVLSEMDNLHRCIKEALRLHPPLIMLMRSSHTD 360
           L+HK+++SAV+EEQ+ LM+KHG K DHD+LSEMD L+R IKEALRLHPPLIML+RSSH+D
Sbjct: 304 LKHKQHLSAVLEEQKNLMQKHGSKVDHDILSEMDTLYRSIKEALRLHPPLIMLLRSSHSD 363

Query: 361 FSVTTKEGKAYDIPKGHIIATSPAFANRLPHVYKDPDRYXXXXXXXXXXEDKAAGPFSYI 420
           FSVTT++GK YDIPKGHI+ATSPAFANRLPH++KDPD Y          EDK AG FSYI
Sbjct: 364 FSVTTRDGKEYDIPKGHIVATSPAFANRLPHIFKDPDSYDPNRFAIGREEDKVAGAFSYI 423

Query: 421 SFGGGRHGCLGEPFAYLQIKAIWSRLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 480
           SFGGGRHGCLGEPFAYLQIKAIWS LLRNFE ELISPFPEIDWNAMVVGVKGKVMVRYKR
Sbjct: 424 SFGGGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGKVMVRYKR 483

Query: 481 RKLSV 486
           R LSV
Sbjct: 484 RVLSV 488

The following BLAST results are available for this feature:
BLAST of Carg15423 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022932660.11.4e-274100.00sterol 14-demethylase-like [Cucurbita moschata] >XP_022932661.1 sterol 14-demeth... [more]
XP_023539138.16.8e-27499.59sterol 14-demethylase-like [Cucurbita pepo subsp. pepo][more]
XP_022972137.13.2e-27198.77sterol 14-demethylase-like [Cucurbita maxima] >XP_022972138.1 sterol 14-demethyl... [more]
XP_022980879.12.3e-26194.23sterol 14-demethylase-like [Cucurbita maxima][more]
XP_023527569.13.0e-26194.23sterol 14-demethylase-like [Cucurbita pepo subsp. pepo][more]
BLAST of Carg15423 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G11680.11.4e-23380.66CYTOCHROME P450 51G1[more]
AT5G45340.12.0e-2524.09cytochrome P450, family 707, subfamily A, polypeptide 3[more]
AT4G19230.22.1e-2223.40cytochrome P450, family 707, subfamily A, polypeptide 1[more]
AT3G61040.16.8e-1828.68cytochrome P450, family 76, subfamily C, polypeptide 7[more]
AT2G24180.11.9e-1529.26cytochrome p450 71b6[more]
BLAST of Carg15423 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9SAA9|CP511_ARATH2.6e-23280.66Sterol 14-demethylase OS=Arabidopsis thaliana OX=3702 GN=CYP51G1 PE=1 SV=1[more]
sp|P93846|CP51_SORBI5.2e-21774.38Obtusifoliol 14-alpha demethylase OS=Sorghum bicolor OX=4558 GN=CYP51 PE=1 SV=1[more]
sp|P93596|CP51_WHEAT1.5e-21176.94Obtusifoliol 14-alpha demethylase (Fragment) OS=Triticum aestivum OX=4565 GN=CYP... [more]
sp|Q1ZXH9|CP51_DICDI3.9e-10343.34Probable lanosterol 14-alpha demethylase OS=Dictyostelium discoideum OX=44689 GN... [more]
sp|Q4PJW3|CP51A_BOVIN4.1e-8940.17Lanosterol 14-alpha demethylase OS=Bos taurus OX=9913 GN=CYP51A1 PE=2 SV=1[more]
BLAST of Carg15423 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3BXE7|A0A1S3BXE7_CUCME1.3e-25792.18sterol 14-demethylase OS=Cucumis melo OX=3656 GN=LOC103494635 PE=3 SV=1[more]
tr|A0A0A0KYP3|A0A0A0KYP3_CUCSA5.0e-25792.18Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa4G007710 PE=2 SV=1[more]
tr|A0A2I4FBQ0|A0A2I4FBQ0_9ROSI1.8e-24384.54sterol 14-demethylase OS=Juglans regia OX=51240 GN=LOC108997326 PE=3 SV=1[more]
tr|A0A2N9FAW8|A0A2N9FAW8_FAGSY1.0e-24184.54Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS12205 PE=3 SV=1[more]
tr|A0A2P4JYN9|A0A2P4JYN9_QUESU3.8e-24184.33Sterol 14-demethylase OS=Quercus suber OX=58331 GN=CFP56_18230 PE=3 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
GO:0004497monooxygenase activity
GO:0005506iron ion binding
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR017972Cyt_P450_CS
IPR036396Cyt_P450_sf
IPR001128Cyt_P450
IPR002403Cyt_P450_E_grp-IV
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15423-RACarg15423-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 36..53
score: 22.22
coord: 333..349
score: 46.61
coord: 58..81
score: 35.1
coord: 275..301
score: 37.32
coord: 413..429
score: 34.39
coord: 371..385
score: 37.18
coord: 387..405
score: 28.54
coord: 429..447
score: 43.52
IPR001128Cytochrome P450PRINTSPR00385P450coord: 284..301
score: 32.57
coord: 420..429
score: 40.95
coord: 338..349
score: 40.69
coord: 429..440
score: 38.9
IPR001128Cytochrome P450PFAMPF00067p450coord: 37..456
e-value: 4.7E-67
score: 226.6
IPR036396Cytochrome P450 superfamilyGENE3DG3DSA:1.10.630.10coord: 27..485
e-value: 1.8E-123
score: 415.0
IPR036396Cytochrome P450 superfamilySUPERFAMILYSSF48264Cytochrome P450coord: 32..481
NoneNo IPR availablePANTHERPTHR24286FAMILY NOT NAMEDcoord: 14..483
NoneNo IPR availablePANTHERPTHR24286:SF58SUBFAMILY NOT NAMEDcoord: 14..483
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 422..431