Carg15037 (gene) Silver-seed gourd

BLAST of Carg15037 vs. NCBI nr
Match: XP_022922598.1 (subtilisin-like protease SBT3.9 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1540.8 bits (3988), Expect = 0.0e+00
Identity = 770/773 (99.61%), Postives = 772/773 (99.87%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60
           MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120
           HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240
           ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240

Query: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300
           GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360
           SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420
           SSVD SFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA
Sbjct: 361 SSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420

Query: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480
           GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ
Sbjct: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480

Query: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600
           VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA
Sbjct: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 660
           VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYN+SAISGITK
Sbjct: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITK 660

Query: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 720
           RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA+K
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAVK 720

Query: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Carg15037 vs. NCBI nr
Match: XP_023551712.1 (subtilisin-like protease SBT3.9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1535.4 bits (3974), Expect = 0.0e+00
Identity = 766/773 (99.09%), Postives = 771/773 (99.74%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60
           MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120
           HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHK+Q
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKIQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240
           ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNS+FQDYWSPRDRSGHGTHVSSVAS
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSKFQDYWSPRDRSGHGTHVSSVAS 240

Query: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300
           GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360
           SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420
           SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA
Sbjct: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420

Query: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480
           GNVVLCFASDD+NDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLD GMQ
Sbjct: 421 GNVVLCFASDDFNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDTGMQ 480

Query: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600
           VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA
Sbjct: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 660
           VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYN+SAISGITK
Sbjct: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITK 660

Query: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 720
           RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA+K
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAVK 720

Query: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           PRILKFNHKMKSI FTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 721 PRILKFNHKMKSIPFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Carg15037 vs. NCBI nr
Match: XP_022984356.1 (subtilisin-like protease SBT3.9 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1510.4 bits (3909), Expect = 0.0e+00
Identity = 757/773 (97.93%), Postives = 766/773 (99.09%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60
           MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120
           HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHK+Q
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKIQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240
           ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDY SPRDRSGHGTHVSSVAS
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYLSPRDRSGHGTHVSSVAS 240

Query: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300
           GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360
           SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420
           SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKF NLVYPEVSDLDDERACESLSLNDTWA+
Sbjct: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFINLVYPEVSDLDDERACESLSLNDTWAS 420

Query: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480
           GNVVLCFASDDYND+THNTS SVKKVGGLGLIVAKNPTKAVEPFI+NFPCVQISLDIGMQ
Sbjct: 421 GNVVLCFASDDYNDNTHNTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDIGMQ 480

Query: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600
           VPPSDPK+RNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA
Sbjct: 541 VPPSDPKNRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 660
           VIFAEG+PMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYN+SAISGI+K
Sbjct: 601 VIFAEGRPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGISK 660

Query: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 720
           RSISCPKRRPSILDINVPSITIPSLTH VSLTRTVTNVGAINSSYKAAIE PPGITIA+K
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHSVSLTRTVTNVGAINSSYKAAIEVPPGITIAVK 720

Query: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           PRILKFNHK KSISFTVTISSN RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 721 PRILKFNHKTKSISFTVTISSNQRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Carg15037 vs. NCBI nr
Match: XP_022141072.1 (subtilisin-like protease SBT3.9 [Momordica charantia])

HSP 1 Score: 1354.0 bits (3503), Expect = 0.0e+00
Identity = 668/775 (86.19%), Postives = 718/775 (92.65%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60
           M SFAHGKSSSTTI L+ IS FT L  LCTL + V AKS+VY+VYMG++PHDNEELLVK 
Sbjct: 1   MSSFAHGKSSSTTILLLFISFFTLLSDLCTLLVPVDAKSHVYVVYMGKRPHDNEELLVKT 60

Query: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120
           HHGVLASVLGSQE   DS+VY+YKYGFSGFAAKLTMAQAQ ISELP+V++VIPNRLHKMQ
Sbjct: 61  HHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQKISELPTVIKVIPNRLHKMQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180
           TTRSWDYLQLSPR  NSLL+KSRMG+GAIIG+LDTGIWPESEVF D GLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTGIWPESEVFSDNGLGPVPSRWKGIC 180

Query: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240
           ESGELFSP KACSRKLIGARYF+KGLEAAYGHP NNS FQDY SPRD +GHGTHVSSVAS
Sbjct: 181 ESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGFQDYLSPRDYAGHGTHVSSVAS 240

Query: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300
           G FV NVSYHGLAVGTVRGGAP SR+AMYKVCWQ+NGGVC+D DILKAIDQAI+DGVDVL
Sbjct: 241 GSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGVCSDADILKAIDQAIHDGVDVL 300

Query: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360
           SLSLGP+FPSYSDVDM NG+AIG FHA+ KGIVVVGAAGN GPAAYSV+NIEPWLLTVAA
Sbjct: 301 SLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAGNYGPAAYSVANIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420
           SSVDRSFLVAITLGNNWTT+GQGMFSGKL +FHNLVYPEVSDL+DERACESLSLNDTWAA
Sbjct: 361 SSVDRSFLVAITLGNNWTTVGQGMFSGKLRRFHNLVYPEVSDLEDERACESLSLNDTWAA 420

Query: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480
           GNVVLCFASDD+ND+TH+TS SVKKVG LGLIVAKNPTK VEPFIN+FPCVQ+SL+IGM 
Sbjct: 421 GNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTKDVEPFINHFPCVQVSLEIGMH 480

Query: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQV IG S TR+GQ LSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600
           VPPSDPKD+NSYAFISGTSMATPHVSAIVALLK+LHSHWSPAAIKSAIVTTAW+SDPYG 
Sbjct: 541 VPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 600

Query: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 660
            IFAEG+P+ SA PFDFGGGIVNPNKAVDPGLVYDMGMADYI+YFCA GYNDSAISGITK
Sbjct: 601 TIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMADYIRYFCAMGYNDSAISGITK 660

Query: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAP--PGITIA 720
           + ISCPKRRPSILD NVPSITIP L H V++TRTVTNVGA+NS+YKAAIE P  PGI IA
Sbjct: 661 KPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVGAVNSTYKAAIETPPGPGIAIA 720

Query: 721 IKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           +KPR+LKFNHK K ISFTVT+SSNHRVTTGYCFGSLTWLDGVHSVRIP+SVRT+I
Sbjct: 721 VKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWLDGVHSVRIPISVRTDI 775

BLAST of Carg15037 vs. NCBI nr
Match: XP_022922599.1 (subtilisin-like protease SBT3.3 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1312.4 bits (3395), Expect = 0.0e+00
Identity = 652/655 (99.54%), Postives = 654/655 (99.85%), Query Frame = 0

Query: 119 MQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKG 178
           MQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKG
Sbjct: 1   MQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKG 60

Query: 179 ICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSV 238
           ICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSV
Sbjct: 61  ICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSV 120

Query: 239 ASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVD 298
           ASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVD
Sbjct: 121 ASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVD 180

Query: 299 VLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTV 358
           VLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTV
Sbjct: 181 VLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTV 240

Query: 359 AASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTW 418
           AASSVD SFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTW
Sbjct: 241 AASSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTW 300

Query: 419 AAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIG 478
           AAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIG
Sbjct: 301 AAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIG 360

Query: 479 MQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAIL 538
           MQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAIL
Sbjct: 361 MQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAIL 420

Query: 539 AAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPY 598
           AAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPY
Sbjct: 421 AAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPY 480

Query: 599 GAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGI 658
           GAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYN+SAISGI
Sbjct: 481 GAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGI 540

Query: 659 TKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA 718
           TKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA
Sbjct: 541 TKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA 600

Query: 719 IKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           +KPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI
Sbjct: 601 VKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 655

BLAST of Carg15037 vs. TAIR10
Match: AT1G32960.1 (Subtilase family protein)

HSP 1 Score: 823.9 bits (2127), Expect = 8.1e-239
Identity = 422/773 (54.59%), Postives = 548/773 (70.89%), Query Frame = 0

Query: 8   KSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLAS 67
           +S  ++I LV +S+ T L+       R   +S V+IVY+G+K H + E + ++HH +LAS
Sbjct: 2   RSFRSSILLVLLSLITVLN---ATRARSETESKVHIVYLGEKKHHDPEFVTESHHQMLAS 61

Query: 68  VLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDY 127
           +LGS++ + DS+VYSY++GFSGFAAKLT +QA+ I++LP VV VIP+  H++ TTR+W+Y
Sbjct: 62  LLGSKKDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEY 121

Query: 128 LQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFS 187
           L LS   P +LL  + MG   IIGV+DTG+WPESE F D G+GP+P +WKG CESGE F 
Sbjct: 122 LGLSSANPKNLLNDTNMGDQVIIGVIDTGVWPESESFNDNGVGPIPRKWKGGCESGENFR 181

Query: 188 PAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNV 247
            +  C+RKLIGA+YF+ G   A     N +E +DY S RD  GHGTHV+S+A G FVPNV
Sbjct: 182 -STDCNRKLIGAKYFINGF-LAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNV 241

Query: 248 SYHGLAVGTVRGGAPHSRIAMYKVCW---QVNGGVCADIDILKAIDQAIYDGVDVLSLSL 307
           SY GLA GT+RGGAP +RIAMYK CW   ++ G  C+D DI+KAID+AI+DGVDVLS+SL
Sbjct: 242 SYKGLAGGTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISL 301

Query: 308 GPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVD 367
               P  S+ D+R+  A G FHAVAKGIVVV A GN GPAA +V NI PW+LTVAA+++D
Sbjct: 302 VGQIPLNSETDIRDEFATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLD 361

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLNDTWA-A 427
           RSF   ITLGNN   +GQ  ++G      +LVYPE +  ++E     CESL+LN  +  A
Sbjct: 362 RSFPTPITLGNNKVILGQATYTGPELGLTSLVYPENARNNNETFSGVCESLNLNPNYTMA 421

Query: 428 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 487
             VVLCF +   N      +S VK  GGLGLI+++NP   + P  ++FPCV +  ++G  
Sbjct: 422 MKVVLCFTASRTNAAISRAASFVKAAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTD 481

Query: 488 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL+YIRSTR+P VKI  S T  GQP+ T V  FSSRGPNS++PAILKPDIAAPGV ILAA
Sbjct: 482 ILSYIRSTRSPVVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAA 541

Query: 548 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 607
             P+D  +   +A +SGTSMATP +S ++ALLK LH  WSPAA +SAIVTTAW +DP+G 
Sbjct: 542 TSPNDTLNVGGFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAIVTTAWRTDPFGE 601

Query: 608 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 667
            IFAEG     + PFD+GGGIVNP KA +PGL+YDMG  DYI Y C+ GYNDS+IS +  
Sbjct: 602 QIFAEGSSRKVSDPFDYGGGIVNPEKAAEPGLIYDMGPQDYILYLCSAGYNDSSISQLVG 661

Query: 668 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 727
           +   C   +PS+LD+N+PSITIP+L   V+LTRTVTNVG ++S YK ++E P G+ + + 
Sbjct: 662 QITVCSNPKPSVLDVNLPSITIPNLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVT 721

Query: 728 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           P  L FN K  S+SFTV +S+ H++ TGY FGSLTW D VH+V IP+SVRT I
Sbjct: 722 PETLVFNSKTISVSFTVRVSTTHKINTGYYFGSLTWTDSVHNVVIPLSVRTQI 769

BLAST of Carg15037 vs. TAIR10
Match: AT4G10520.1 (Subtilase family protein)

HSP 1 Score: 815.5 bits (2105), Expect = 2.9e-236
Identity = 422/740 (57.03%), Postives = 540/740 (72.97%), Query Frame = 0

Query: 35  VAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKL 94
           V A+S VY+VY+G+K HDN E + ++HH +L S+LGS+E  +DS+VYSY++GFSGFAAKL
Sbjct: 23  VVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKL 82

Query: 95  TMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLD 154
           T +QAQ ISELP VV+VIPN L++M TTR+WDYL +SP   +SLL+K+ MG   I+GV+D
Sbjct: 83  TESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDSLLQKANMGYNVIVGVID 142

Query: 155 TGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPL 214
           +G+WPESE+F D+G GP+PSRWKG CESGELF+ +  C+RKLIGA+YFV GL A +G  +
Sbjct: 143 SGVWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFG-VV 202

Query: 215 NNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQ 274
           N ++  +Y SPRD +GHGTHV+S   G F+PNVSY GL  GT RGGAP   IA+YK CW 
Sbjct: 203 NRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACW- 262

Query: 275 VNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVV 334
              G C+  D+LKA+D+AI+DGVD+LSLSLGPS P + + +     ++GAFHAVAKGI V
Sbjct: 263 --SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETE---HTSVGAFHAVAKGIPV 322

Query: 335 VGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHN 394
           V AAGN+GP A ++SN+ PW+LTVAA++ DRSF  AITLGNN T +GQ ++ G    F  
Sbjct: 323 VIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIYGGPELGFVG 382

Query: 395 LVYPEVSDLDDERACESLSLN-DTWAAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIV 454
           L YPE S L  +  CE LS N ++   G VVLCFA+   +  ++   ++V   GGLGLI+
Sbjct: 383 LTYPE-SPLSGD--CEKLSANPNSTMEGKVVLCFAA---STPSNAAIAAVINAGGLGLIM 442

Query: 455 AKNPTKAVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYF 514
           AKNPT ++ P    FP V I  ++G  IL YIRSTR+P VKI AS T  GQ +ST VA F
Sbjct: 443 AKNPTHSLTP-TRKFPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVATF 502

Query: 515 SSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLK 574
           SSRGPNSV+PAILKPDIAAPGV ILAA+ P+   +   +A +SGTSMATP VS +V LLK
Sbjct: 503 SSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLLK 562

Query: 575 TLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLV 634
           +LH  WSP+AIKSAIVTTAW +DP G  IFA+G     A PFD+GGG++NP KAV PGL+
Sbjct: 563 SLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLI 622

Query: 635 YDMGMADYIQYFCAKGYNDSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTR 694
           YDM   DY+ Y C+  Y+D +IS +  +   CP  +PS+LD+N+PSITIP+L   V+LTR
Sbjct: 623 YDMTTDDYVMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTR 682

Query: 695 TVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGS 754
           TVTNVG +NS YK  I+ P GI +A+ P  L F++     SFTV +S+ H+V TGY FGS
Sbjct: 683 TVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYFGS 742

Query: 755 LTWLDGVHSVRIPVSVRTNI 774
           LTW D +H+V IPVSVRT I
Sbjct: 743 LTWTDNMHNVAIPVSVRTQI 748

BLAST of Carg15037 vs. TAIR10
Match: AT4G10550.3 (Subtilase family protein)

HSP 1 Score: 806.6 bits (2082), Expect = 1.3e-233
Identity = 410/773 (53.04%), Postives = 539/773 (69.73%), Query Frame = 0

Query: 8   KSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLAS 67
           KSS  TIFL     F A           +AK  V+IVY+G+K HD+ E + ++HH +L S
Sbjct: 26  KSSKETIFLTKERSFVA---------ESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWS 85

Query: 68  VLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDY 127
           +LGS+E + DS+VYSY++GFSGFAAKLT +QA+ I++LP VV VIP+  +K+ TTR+WDY
Sbjct: 86  LLGSKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDY 145

Query: 128 LQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFS 187
           L LS   P SLL ++ MG   IIGV+DTG+WPESEVF D G GPVPS WKG CE+GE F+
Sbjct: 146 LGLSAANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFN 205

Query: 188 PAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNV 247
            +  C++KLIGA+YF+ G   A     N++   D+ SPRD  GHGTHVS++A G FVPN+
Sbjct: 206 SSN-CNKKLIGAKYFINGF-LAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNI 265

Query: 248 SYHGLAVGTVRGGAPHSRIAMYKVCWQVNGG---VCADIDILKAIDQAIYDGVDVLSLSL 307
           SY GLA GTVRGGAP + IAMYK CW ++      C+  DILKA+D+A++DGVDVLS+SL
Sbjct: 266 SYKGLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISL 325

Query: 308 GPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVD 367
           G S P Y + D+R+GI  GAFHAV KGI VV + GNSGP + +V+N  PW++TVAA+++D
Sbjct: 326 GSSVPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLD 385

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLN-DTWAA 427
           RSF   +TLGNN   +GQ M++G    F +LVYPE     +E     CE L  N +    
Sbjct: 386 RSFATPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTME 445

Query: 428 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 487
           G VVLCF +  Y     + +  VK+ GGLG+I+A++P  A++P +++FPCV +  ++G  
Sbjct: 446 GKVVLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTD 505

Query: 488 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL Y RS+ +P VKI  S T VGQP+ T VA FSSRGPNS+APAILKPDIAAPGV+ILAA
Sbjct: 506 ILLYTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAA 565

Query: 548 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 607
              +   D+  +  +SGTSMA P +S + ALLK LH  WSPAAI+SAIVTTAW +DP+G 
Sbjct: 566 TTNTTFSDQ-GFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGE 625

Query: 608 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 667
            IFAEG P   A PFD+GGG+VNP K+ +PGLVYDMG+ DY+ Y C+ GYN+++IS +  
Sbjct: 626 QIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIG 685

Query: 668 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 727
           ++  C   +PS+LD N+PSITIP+L   V++TRTVTNVG +NS Y+  +E P G  + + 
Sbjct: 686 KTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVT 745

Query: 728 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           P  L FN   K + F V +S+ H+  TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 746 PETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQI 786

BLAST of Carg15037 vs. TAIR10
Match: AT4G10540.1 (Subtilase family protein)

HSP 1 Score: 802.7 bits (2072), Expect = 1.9e-232
Identity = 413/773 (53.43%), Postives = 539/773 (69.73%), Query Frame = 0

Query: 8   KSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLAS 67
           KS  T IF+  I     L+ L T      A+S V+IVY+G+K HD+ E + ++HH +L S
Sbjct: 2   KSCRTLIFVAII-----LNGLSTFVAHAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWS 61

Query: 68  VLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDY 127
           +LGS+E +  S+V+SY++GFSGFAAKLT +QA+ +++LP VV V P+  +++ TTR+WDY
Sbjct: 62  LLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDY 121

Query: 128 LQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFS 187
           L LS   P +LL  + MG   IIG++D+G+WPESEVF D G+GPVPS WKG C SGE F+
Sbjct: 122 LGLSVANPKNLLNDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFT 181

Query: 188 PAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNV 247
            ++ C++KLIGA+YF+ G  A +    N++E  D+ SPRDRSGHGTHV+++A G +VP++
Sbjct: 182 SSQ-CNKKLIGAKYFINGFLATH-ESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSI 241

Query: 248 SYHGLAVGTVRGGAPHSRIAMYKVCWQVNG---GVCADIDILKAIDQAIYDGVDVLSLSL 307
           SY GLA GTVRGGAP +RIAMYK CW ++      C+  DILKA+D+A++DGVDVLSLS+
Sbjct: 242 SYKGLAGGTVRGGAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSI 301

Query: 308 GPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVD 367
           G  FP + + D+R  IA GAFHAV KGI VV + GNSGPAA +V N  PW+LTVAA+++D
Sbjct: 302 GYRFPYFPETDVRAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLD 361

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLNDTWA-A 427
           RSF   ITLGNN   +GQ M++G    F +LVYPE     +E     CE L  N     A
Sbjct: 362 RSFPTPITLGNNKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMA 421

Query: 428 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 487
           G VVLCF +        +  S VK+ GGLG+IVA+NP   + P  ++FPCV +  ++G  
Sbjct: 422 GKVVLCFTTSTRYITVSSAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTD 481

Query: 488 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL YIRST  P VKI  S T VGQP+ T VA FSSRGPNS+ PAILKPDIAAPGV+ILAA
Sbjct: 482 ILLYIRSTGLPVVKIQPSKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAA 541

Query: 548 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 607
              +   +   + F+SGTSMA P +S +VALLK LH  WSPAAI+SAIVTTAW +DP+G 
Sbjct: 542 TTTNKTFNDRGFIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFGE 601

Query: 608 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 667
            IFAEG P   A PFD+GGG+VNP KA  PGLVYD+G+ DY+ Y C+ GYN+++IS +  
Sbjct: 602 QIFAEGSPRKLADPFDYGGGLVNPEKAAKPGLVYDLGLEDYVLYMCSVGYNETSISQLVG 661

Query: 668 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 727
           +   C   +PS+LD N+PSITIP+L   V+LTRT+TNVG + S YK  IE P GI + + 
Sbjct: 662 KGTVCSNPKPSVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVT 721

Query: 728 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           P  L FN   K +SF V +S+ H++ TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722 PETLLFNSTTKRVSFKVKVSTTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQI 767

BLAST of Carg15037 vs. TAIR10
Match: AT1G32940.1 (Subtilase family protein)

HSP 1 Score: 798.1 bits (2060), Expect = 4.7e-231
Identity = 401/748 (53.61%), Postives = 527/748 (70.45%), Query Frame = 0

Query: 33  IRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAA 92
           +R + +S V+IVY+G+K HD+ E + ++HH +L+S+LGS+  + +S+VYSY++GFSGFAA
Sbjct: 21  VRASDESKVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGFAA 80

Query: 93  KLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGV 152
           KLT +QA+ +++ P VV V+ +  +++ TTR+WDYL LS   PN+LL  + MG   IIG 
Sbjct: 81  KLTESQAKKLADSPEVVHVMADSFYELATTRTWDYLGLSVANPNNLLNDTNMGDQVIIGF 140

Query: 153 LDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGH 212
           +DTG+WPESE F D G+GP+PS WKG CESGE F     C+RKLIGA+YF+ G   A   
Sbjct: 141 IDTGVWPESESFNDNGVGPIPSHWKGGCESGEKFISTN-CNRKLIGAKYFINGF-LAENE 200

Query: 213 PLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVC 272
             N +E +DY S RD  GHGTH +S+A G FVPN+SY GLA G +RGGAP +RIA+YK C
Sbjct: 201 GFNTTESRDYISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNLRGGAPRARIAIYKAC 260

Query: 273 W---QVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVA 332
           W   Q+    C+  DILKA+D++++DGVDVLSLSLG   P Y + D+R+ IA GAFHAVA
Sbjct: 261 WYVDQLGAVACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLRDRIATGAFHAVA 320

Query: 333 KGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKL 392
           KGI+VV A GNSGPAA +V N  PW++TVAA+++DRSF   ITLGN    +GQ +++G+ 
Sbjct: 321 KGIIVVCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPITLGNRKVILGQALYTGQE 380

Query: 393 TKFHNLVYPEVSDLDDER---ACESLSLN-DTWAAGNVVLCFASDDYNDDTHNTSSSVKK 452
             F +LVYPE +   +E     CE L+LN +   AG VVLCF ++         +S VK 
Sbjct: 381 LGFTSLVYPENAGFTNETFSGVCERLNLNPNRTMAGKVVLCFTTNTLFTAVSRAASYVKA 440

Query: 453 VGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQP 512
            GGLG+I+A+NP   + P  ++FPCV I  ++G  +L YIRSTR+P VKI  S T VGQP
Sbjct: 441 AGGLGVIIARNPGYNLTPCRDDFPCVAIDYELGTDVLLYIRSTRSPVVKIQPSRTLVGQP 500

Query: 513 LSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHV 572
           + T VA FSSRGPNS++PAILKPDI APGV+ILAA  P        +  ++GTSMA P V
Sbjct: 501 VGTKVATFSSRGPNSISPAILKPDIGAPGVSILAATSPDSNSSVGGFDILAGTSMAAPVV 560

Query: 573 SAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPN 632
           + +VALLK LH +WSPAA +SAIVTTAW +DP+G  IFAEG     A PFD+GGGIVNP 
Sbjct: 561 AGVVALLKALHPNWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKVADPFDYGGGIVNPE 620

Query: 633 KAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITKRSISCPKRRPSILDINVPSITIPSL 692
           KA DPGL+YDMG  DYI Y C+ GYNDS+I+ +      C   + S+LD+N+PSITIP L
Sbjct: 621 KAADPGLIYDMGPRDYILYLCSAGYNDSSITQLVGNVTVCSTPKTSVLDVNLPSITIPDL 680

Query: 693 THPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRV 752
              V+LTRTVTNVG ++S YK  +E P GI + + P  L FN K K++SFTV +S+ H++
Sbjct: 681 KDEVTLTRTVTNVGTVDSVYKVVVEPPLGIQVVVAPETLVFNSKTKNVSFTVRVSTTHKI 740

Query: 753 TTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
            TG+ FG+L W D +H+V IPVSVRT I
Sbjct: 741 NTGFYFGNLIWTDSMHNVTIPVSVRTQI 766

BLAST of Carg15037 vs. Swiss-Prot
Match: sp|Q9MAP5|SBT33_ARATH (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 1.5e-237
Identity = 422/773 (54.59%), Postives = 548/773 (70.89%), Query Frame = 0

Query: 8   KSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLAS 67
           +S  ++I LV +S+ T L+       R   +S V+IVY+G+K H + E + ++HH +LAS
Sbjct: 2   RSFRSSILLVLLSLITVLN---ATRARSETESKVHIVYLGEKKHHDPEFVTESHHQMLAS 61

Query: 68  VLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDY 127
           +LGS++ + DS+VYSY++GFSGFAAKLT +QA+ I++LP VV VIP+  H++ TTR+W+Y
Sbjct: 62  LLGSKKDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEY 121

Query: 128 LQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFS 187
           L LS   P +LL  + MG   IIGV+DTG+WPESE F D G+GP+P +WKG CESGE F 
Sbjct: 122 LGLSSANPKNLLNDTNMGDQVIIGVIDTGVWPESESFNDNGVGPIPRKWKGGCESGENFR 181

Query: 188 PAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNV 247
            +  C+RKLIGA+YF+ G   A     N +E +DY S RD  GHGTHV+S+A G FVPNV
Sbjct: 182 -STDCNRKLIGAKYFINGF-LAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNV 241

Query: 248 SYHGLAVGTVRGGAPHSRIAMYKVCW---QVNGGVCADIDILKAIDQAIYDGVDVLSLSL 307
           SY GLA GT+RGGAP +RIAMYK CW   ++ G  C+D DI+KAID+AI+DGVDVLS+SL
Sbjct: 242 SYKGLAGGTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISL 301

Query: 308 GPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVD 367
               P  S+ D+R+  A G FHAVAKGIVVV A GN GPAA +V NI PW+LTVAA+++D
Sbjct: 302 VGQIPLNSETDIRDEFATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLD 361

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLNDTWA-A 427
           RSF   ITLGNN   +GQ  ++G      +LVYPE +  ++E     CESL+LN  +  A
Sbjct: 362 RSFPTPITLGNNKVILGQATYTGPELGLTSLVYPENARNNNETFSGVCESLNLNPNYTMA 421

Query: 428 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 487
             VVLCF +   N      +S VK  GGLGLI+++NP   + P  ++FPCV +  ++G  
Sbjct: 422 MKVVLCFTASRTNAAISRAASFVKAAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTD 481

Query: 488 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL+YIRSTR+P VKI  S T  GQP+ T V  FSSRGPNS++PAILKPDIAAPGV ILAA
Sbjct: 482 ILSYIRSTRSPVVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAA 541

Query: 548 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 607
             P+D  +   +A +SGTSMATP +S ++ALLK LH  WSPAA +SAIVTTAW +DP+G 
Sbjct: 542 TSPNDTLNVGGFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAIVTTAWRTDPFGE 601

Query: 608 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 667
            IFAEG     + PFD+GGGIVNP KA +PGL+YDMG  DYI Y C+ GYNDS+IS +  
Sbjct: 602 QIFAEGSSRKVSDPFDYGGGIVNPEKAAEPGLIYDMGPQDYILYLCSAGYNDSSISQLVG 661

Query: 668 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 727
           +   C   +PS+LD+N+PSITIP+L   V+LTRTVTNVG ++S YK ++E P G+ + + 
Sbjct: 662 QITVCSNPKPSVLDVNLPSITIPNLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVT 721

Query: 728 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           P  L FN K  S+SFTV +S+ H++ TGY FGSLTW D VH+V IP+SVRT I
Sbjct: 722 PETLVFNSKTISVSFTVRVSTTHKINTGYYFGSLTWTDSVHNVVIPLSVRTQI 769

BLAST of Carg15037 vs. Swiss-Prot
Match: sp|Q9ZSB0|SBT39_ARATH (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 5.2e-235
Identity = 422/740 (57.03%), Postives = 540/740 (72.97%), Query Frame = 0

Query: 35  VAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKL 94
           V A+S VY+VY+G+K HDN E + ++HH +L S+LGS+E  +DS+VYSY++GFSGFAAKL
Sbjct: 23  VVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKL 82

Query: 95  TMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLD 154
           T +QAQ ISELP VV+VIPN L++M TTR+WDYL +SP   +SLL+K+ MG   I+GV+D
Sbjct: 83  TESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDSLLQKANMGYNVIVGVID 142

Query: 155 TGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPL 214
           +G+WPESE+F D+G GP+PSRWKG CESGELF+ +  C+RKLIGA+YFV GL A +G  +
Sbjct: 143 SGVWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFG-VV 202

Query: 215 NNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQ 274
           N ++  +Y SPRD +GHGTHV+S   G F+PNVSY GL  GT RGGAP   IA+YK CW 
Sbjct: 203 NRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACW- 262

Query: 275 VNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVV 334
              G C+  D+LKA+D+AI+DGVD+LSLSLGPS P + + +     ++GAFHAVAKGI V
Sbjct: 263 --SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETE---HTSVGAFHAVAKGIPV 322

Query: 335 VGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHN 394
           V AAGN+GP A ++SN+ PW+LTVAA++ DRSF  AITLGNN T +GQ ++ G    F  
Sbjct: 323 VIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIYGGPELGFVG 382

Query: 395 LVYPEVSDLDDERACESLSLN-DTWAAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIV 454
           L YPE S L  +  CE LS N ++   G VVLCFA+   +  ++   ++V   GGLGLI+
Sbjct: 383 LTYPE-SPLSGD--CEKLSANPNSTMEGKVVLCFAA---STPSNAAIAAVINAGGLGLIM 442

Query: 455 AKNPTKAVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYF 514
           AKNPT ++ P    FP V I  ++G  IL YIRSTR+P VKI AS T  GQ +ST VA F
Sbjct: 443 AKNPTHSLTP-TRKFPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVATF 502

Query: 515 SSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLK 574
           SSRGPNSV+PAILKPDIAAPGV ILAA+ P+   +   +A +SGTSMATP VS +V LLK
Sbjct: 503 SSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLLK 562

Query: 575 TLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLV 634
           +LH  WSP+AIKSAIVTTAW +DP G  IFA+G     A PFD+GGG++NP KAV PGL+
Sbjct: 563 SLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLI 622

Query: 635 YDMGMADYIQYFCAKGYNDSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTR 694
           YDM   DY+ Y C+  Y+D +IS +  +   CP  +PS+LD+N+PSITIP+L   V+LTR
Sbjct: 623 YDMTTDDYVMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTR 682

Query: 695 TVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGS 754
           TVTNVG +NS YK  I+ P GI +A+ P  L F++     SFTV +S+ H+V TGY FGS
Sbjct: 683 TVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYFGS 742

Query: 755 LTWLDGVHSVRIPVSVRTNI 774
           LTW D +H+V IPVSVRT I
Sbjct: 743 LTWTDNMHNVAIPVSVRTQI 748

BLAST of Carg15037 vs. Swiss-Prot
Match: sp|Q9ZSB1|SBT3A_ARATH (Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3 SV=2)

HSP 1 Score: 812.8 bits (2098), Expect = 3.4e-234
Identity = 422/740 (57.03%), Postives = 534/740 (72.16%), Query Frame = 0

Query: 35  VAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKL 94
           V A+S VY+VY+G+K HDN E + ++HH +L S+LGS+E  +DS+VYSY++GFSGFAAKL
Sbjct: 23  VVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKL 82

Query: 95  TMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLD 154
           T +QAQ ISELP VV+VIPN L++M TTR+WDYL +SP   +SLL+K+ MG   I+GV+D
Sbjct: 83  TESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDSLLQKANMGYNVIVGVID 142

Query: 155 TGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPL 214
           TG+WPESE+F D+G GP+PSRWKG CESGELF+ +  C+RKLIGA+YF+    A +G  L
Sbjct: 143 TGVWPESEMFNDKGYGPIPSRWKGGCESGELFNGSIHCNRKLIGAKYFIDANNAQFG-VL 202

Query: 215 NNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQ 274
           N +E  DY SPRD +GHGTHV+S   G F+PNVSY GL  GT RGGAP   IA+YK CW 
Sbjct: 203 NKTENPDYLSPRDFNGHGTHVASTIGGSFLPNVSYLGLGRGTARGGAPGVHIAVYKACWV 262

Query: 275 VNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVV 334
             G  C+  D+LKA+D+AI+DGVD+LSLSL  S P + + D R   ++GAFHAVAKGI V
Sbjct: 263 QRG--CSGADVLKAMDEAIHDGVDILSLSLQTSVPLFPETDARELTSVGAFHAVAKGIPV 322

Query: 335 VGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHN 394
           V AA N+GP A ++SN+ PW+LTVAA++ DRSF  AITLGNN T +GQ +F G    F  
Sbjct: 323 VAAASNAGPTAQTLSNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIFGGSELGFVG 382

Query: 395 LVYPEVSDLDDERACESLSLNDTWA-AGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIV 454
           L YPE S L  +  CE LS N   A  G VVLCFA+   +  ++   ++V   GGLGLI+
Sbjct: 383 LTYPE-SPLSGD--CEKLSANPKSAMEGKVVLCFAA---STPSNAAITAVINAGGLGLIM 442

Query: 455 AKNPTKAVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYF 514
           A+NPT  + P + NFP V +  ++G  IL YIRSTR+P V I AS T  GQ +ST VA F
Sbjct: 443 ARNPTHLLRP-LRNFPYVSVDFELGTDILFYIRSTRSPIVNIQASRTLFGQSVSTKVATF 502

Query: 515 SSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLK 574
           SSRGPNSV+PAILKPDIAAPGV ILAA+ P+   D   +A +SGTSMATP VS +V LLK
Sbjct: 503 SSRGPNSVSPAILKPDIAAPGVNILAAISPNSIND-GGFAMMSGTSMATPVVSGVVVLLK 562

Query: 575 TLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLV 634
           +LH  WSP+AIKSAIVTTAW +DP G  IFA+G     A PFD+GGG++NP KAV PGL+
Sbjct: 563 SLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLI 622

Query: 635 YDMGMADYIQYFCAKGYNDSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTR 694
           YDM   DY+ Y C+  Y+D +IS +  +   CP  +PS+LD+N+PSITIP+L   V+LTR
Sbjct: 623 YDMTTDDYVMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTR 682

Query: 695 TVTNVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGS 754
           TVTNVG +NS YK  I+ P G+ +A+ P  L F+      SFTV +S+ H+V TGY FGS
Sbjct: 683 TVTNVGPVNSVYKVVIDPPTGVNVAVTPTELVFDSTTTKRSFTVRVSTTHKVNTGYYFGS 742

Query: 755 LTWLDGVHSVRIPVSVRTNI 774
           LTW D +H+V IPVSVRT I
Sbjct: 743 LTWTDTLHNVAIPVSVRTQI 751

BLAST of Carg15037 vs. Swiss-Prot
Match: sp|Q9SZY3|SBT38_ARATH (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 3.5e-231
Identity = 413/773 (53.43%), Postives = 539/773 (69.73%), Query Frame = 0

Query: 8   KSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLAS 67
           KS  T IF+  I     L+ L T      A+S V+IVY+G+K HD+ E + ++HH +L S
Sbjct: 2   KSCRTLIFVAII-----LNGLSTFVAHAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWS 61

Query: 68  VLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDY 127
           +LGS+E +  S+V+SY++GFSGFAAKLT +QA+ +++LP VV V P+  +++ TTR+WDY
Sbjct: 62  LLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDY 121

Query: 128 LQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFS 187
           L LS   P +LL  + MG   IIG++D+G+WPESEVF D G+GPVPS WKG C SGE F+
Sbjct: 122 LGLSVANPKNLLNDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFT 181

Query: 188 PAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNV 247
            ++ C++KLIGA+YF+ G  A +    N++E  D+ SPRDRSGHGTHV+++A G +VP++
Sbjct: 182 SSQ-CNKKLIGAKYFINGFLATH-ESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSI 241

Query: 248 SYHGLAVGTVRGGAPHSRIAMYKVCWQVNG---GVCADIDILKAIDQAIYDGVDVLSLSL 307
           SY GLA GTVRGGAP +RIAMYK CW ++      C+  DILKA+D+A++DGVDVLSLS+
Sbjct: 242 SYKGLAGGTVRGGAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSI 301

Query: 308 GPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVD 367
           G  FP + + D+R  IA GAFHAV KGI VV + GNSGPAA +V N  PW+LTVAA+++D
Sbjct: 302 GYRFPYFPETDVRAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLD 361

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLNDTWA-A 427
           RSF   ITLGNN   +GQ M++G    F +LVYPE     +E     CE L  N     A
Sbjct: 362 RSFPTPITLGNNKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMA 421

Query: 428 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 487
           G VVLCF +        +  S VK+ GGLG+IVA+NP   + P  ++FPCV +  ++G  
Sbjct: 422 GKVVLCFTTSTRYITVSSAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTD 481

Query: 488 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL YIRST  P VKI  S T VGQP+ T VA FSSRGPNS+ PAILKPDIAAPGV+ILAA
Sbjct: 482 ILLYIRSTGLPVVKIQPSKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAA 541

Query: 548 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 607
              +   +   + F+SGTSMA P +S +VALLK LH  WSPAAI+SAIVTTAW +DP+G 
Sbjct: 542 TTTNKTFNDRGFIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFGE 601

Query: 608 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 667
            IFAEG P   A PFD+GGG+VNP KA  PGLVYD+G+ DY+ Y C+ GYN+++IS +  
Sbjct: 602 QIFAEGSPRKLADPFDYGGGLVNPEKAAKPGLVYDLGLEDYVLYMCSVGYNETSISQLVG 661

Query: 668 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 727
           +   C   +PS+LD N+PSITIP+L   V+LTRT+TNVG + S YK  IE P GI + + 
Sbjct: 662 KGTVCSNPKPSVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVT 721

Query: 728 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           P  L FN   K +SF V +S+ H++ TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722 PETLLFNSTTKRVSFKVKVSTTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQI 767

BLAST of Carg15037 vs. Swiss-Prot
Match: sp|Q8L7I2|SBT36_ARATH (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 4.5e-231
Identity = 406/769 (52.80%), Postives = 542/769 (70.48%), Query Frame = 0

Query: 12  TTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGS 71
           T+I++V +S+   L+   +     +AK  V+IVY+G+K HD+ E + ++HH +L S+LGS
Sbjct: 6   TSIYVV-LSLVIFLNVQRSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGS 65

Query: 72  QEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLS 131
           +E + DS+VYSY++GFSGFAAKLT +QA+ I++LP VV VIP+  +K+ TTR+WDYL LS
Sbjct: 66  KEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLS 125

Query: 132 PRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKA 191
              P SLL ++ MG   IIGV+DTG+WPESEVF D G GPVPS WKG CE+GE F+ +  
Sbjct: 126 AANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSN- 185

Query: 192 CSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHG 251
           C++KLIGA+YF+ G   A     N++   D+ SPRD  GHGTHVS++A G FVPN+SY G
Sbjct: 186 CNKKLIGAKYFINGF-LAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKG 245

Query: 252 LAVGTVRGGAPHSRIAMYKVCWQVNGG---VCADIDILKAIDQAIYDGVDVLSLSLGPSF 311
           LA GTVRGGAP + IAMYK CW ++      C+  DILKA+D+A++DGVDVLS+SLG S 
Sbjct: 246 LAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSV 305

Query: 312 PSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFL 371
           P Y + D+R+GI  GAFHAV KGI VV + GNSGP + +V+N  PW++TVAA+++DRSF 
Sbjct: 306 PLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFA 365

Query: 372 VAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDER---ACESLSLN-DTWAAGNVV 431
             +TLGNN   +GQ M++G    F +LVYPE     +E     CE L  N +    G VV
Sbjct: 366 TPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVV 425

Query: 432 LCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQILNY 491
           LCF +  Y     + +  VK+ GGLG+I+A++P  A++P +++FPCV +  ++G  IL Y
Sbjct: 426 LCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLY 485

Query: 492 IRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPS 551
            RS+ +P VKI  S T VGQP+ T VA FSSRGPNS+APAILKPDIAAPGV+ILAA   +
Sbjct: 486 TRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAATTNT 545

Query: 552 DPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFA 611
              D+  +  +SGTSMA P +S + ALLK LH  WSPAAI+SAIVTTAW +DP+G  IFA
Sbjct: 546 TFSDQ-GFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFA 605

Query: 612 EGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITKRSIS 671
           EG P   A PFD+GGG+VNP K+ +PGLVYDMG+ DY+ Y C+ GYN+++IS +  ++  
Sbjct: 606 EGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTTV 665

Query: 672 CPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIKPRIL 731
           C   +PS+LD N+PSITIP+L   V++TRTVTNVG +NS Y+  +E P G  + + P  L
Sbjct: 666 CSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETL 725

Query: 732 KFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
            FN   K + F V +S+ H+  TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 726 VFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQI 770

BLAST of Carg15037 vs. TrEMBL
Match: tr|A0A2P4M3J9|A0A2P4M3J9_QUESU (Subtilisin-like protease sbt3.8 OS=Quercus suber OX=58331 GN=CFP56_41580 PE=4 SV=1)

HSP 1 Score: 986.5 bits (2549), Expect = 3.4e-284
Identity = 493/774 (63.70%), Postives = 607/774 (78.42%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60
           M S    KSS+ T+ LV + + + L+   T+     AKSNVYIVYMG + HD+ ELL + 
Sbjct: 1   MNSVRCSKSSNRTLSLVFLCLLSFLNEQHTVLTLAEAKSNVYIVYMGARQHDDPELLAET 60

Query: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120
           HH +L  VLGS E S +++VYSYK+GFSGFAAKLT AQAQ ISELP+V++VIPN  HK+Q
Sbjct: 61  HHTMLTRVLGSIEASAEAMVYSYKHGFSGFAAKLTKAQAQAISELPTVLQVIPNLFHKLQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180
           TTRSWDYLQLS     ++L KS+MG+G IIG++DTGIWPES+VF DEGLGP+PSRW+GIC
Sbjct: 121 TTRSWDYLQLSSCSTTNVLHKSKMGNGVIIGLIDTGIWPESQVFRDEGLGPIPSRWRGIC 180

Query: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240
           ESGELF+    C+RKLIGARYF+KGL+A YG P N++E+Q+  SPRDR GHGT  SS+A 
Sbjct: 181 ESGELFNTRMTCNRKLIGARYFIKGLQAEYGQPYNSTEYQECLSPRDRLGHGTSTSSIAC 240

Query: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQV-NGGVCADIDILKAIDQAIYDGVDV 300
           G FV NVSY+GL VGTVRGGAP +R+AMYKVCW +  GGVC   DILKA D+AI+DGVDV
Sbjct: 241 GSFVANVSYNGLGVGTVRGGAPLARLAMYKVCWNLYGGGVCTGADILKAFDEAIHDGVDV 300

Query: 301 LSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVA 360
           LSLS+ P  P + D+++ NGI+IGA+HAVAKGI VV AAGN+GP+A ++ N  PW++TVA
Sbjct: 301 LSLSIAPDLP-FPDLEIHNGISIGAYHAVAKGITVVCAAGNAGPSAQTIQNTSPWIITVA 360

Query: 361 ASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWA 420
           AS++DRSF   ITLGNNWTTMGQ MF+GK T F NLVYPEV +L   R CESL+ NDTW 
Sbjct: 361 ASTIDRSFPTPITLGNNWTTMGQAMFTGKDTGFINLVYPEVPELLSPRHCESLTPNDTWL 420

Query: 421 AGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGM 480
           AGNV LCF S+   +   + + SVK +G  GLI+A NP++++    +NFPCVQ+S +IG 
Sbjct: 421 AGNVALCFTSNSNQNAVEDAAWSVKDIGCSGLIIAINPSRSLHSCDDNFPCVQVSYEIGT 480

Query: 481 QILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILA 540
           QIL YIRSTRNP V+I  S T +G+P+ST VAYFSSRGP+S++PAILKPD+AAPGV ILA
Sbjct: 481 QILYYIRSTRNPLVRIRPSKTHLGRPVSTNVAYFSSRGPSSISPAILKPDVAAPGVHILA 540

Query: 541 AVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYG 600
           AV P +P+   +Y F+SGTSMATPHVS IVALLK+LH  WSPAAIKSA+VTTAW++DP G
Sbjct: 541 AVSPHNPE--IAYKFLSGTSMATPHVSGIVALLKSLHPDWSPAAIKSALVTTAWTTDPSG 600

Query: 601 AVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGIT 660
             +FAEG+PM  A PFD+GGGIVN N+A DPGLVYDMG ADYIQY CA G N+SAIS +T
Sbjct: 601 EPVFAEGEPMKLADPFDYGGGIVNSNRAADPGLVYDMGTADYIQYLCAMGNNNSAISQLT 660

Query: 661 KRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAI 720
           +    CP ++PSILD+N+PSITIP+L +  +LTRTVTNVGA+NS Y A IE P GITIA+
Sbjct: 661 EHPTFCPIKQPSILDVNLPSITIPTLGNSTTLTRTVTNVGAVNSRYVAIIEPPRGITIAV 720

Query: 721 KPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           KP  L FN  +K+ISFTV +SS  +V+TGY FGSLTW DG+H+VR P+SVRT I
Sbjct: 721 KPNTLIFNSMIKTISFTVRVSSTQKVSTGYSFGSLTWTDGMHTVRSPISVRTEI 771

BLAST of Carg15037 vs. TrEMBL
Match: tr|A0A2P5EDQ4|A0A2P5EDQ4_9ROSA (Subtilase OS=Trema orientalis OX=63057 GN=TorSBT24 PE=4 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.1e-274
Identity = 476/774 (61.50%), Postives = 592/774 (76.49%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60
           MGS    K +   +  + +S+F+ ++    L      KSNVYI++MGQK H++ +LL K 
Sbjct: 5   MGSSLQMKKNGAALLFLFVSLFSIMNEHYLL-TAADPKSNVYIIHMGQKQHEDVKLLKKT 64

Query: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120
           HH +LA+VLGS+E S  +++YSYK+GFSGFAAKLT  QAQ +SELP V  VIPN+L+K+Q
Sbjct: 65  HHELLATVLGSKEESKRAMIYSYKHGFSGFAAKLTKTQAQTVSELPGVALVIPNQLYKVQ 124

Query: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180
           TTRSWDYLQLS R      +KS++G+GAIIG+LDTGIWPESE F D+GLGP+PSRWKG+C
Sbjct: 125 TTRSWDYLQLSSRDQTHFEQKSKLGNGAIIGLLDTGIWPESEAFSDKGLGPIPSRWKGVC 184

Query: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240
           +SG+ F  AK C+RKLIGARYF+K LEA YG P N +E  DY SPRD SGHGTH S++A+
Sbjct: 185 QSGQNFDGAKHCNRKLIGARYFIKALEAEYGKPYNATEAGDYLSPRDSSGHGTHTSTIAA 244

Query: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQV-NGGVCADIDILKAIDQAIYDGVDV 300
           G  + N SY+GL VGTVRGGA  +R+AMYKVCW +  GG+CA  DILKA D+A+ DGVDV
Sbjct: 245 GSSMANRSYNGLGVGTVRGGATQARVAMYKVCWSLPTGGICAGADILKAFDEAVRDGVDV 304

Query: 301 LSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVA 360
           +S+S+    PS+ +VD R+ IA+GAFHAVA+G+VVV +AGN+GP+  +V N  PW+LTVA
Sbjct: 305 VSVSIAADVPSFHEVDERSAIAVGAFHAVAQGVVVVCSAGNAGPSPQTVQNASPWILTVA 364

Query: 361 ASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWA 420
           ASSVDRSF   +TLG NWTT GQ MF+G  T F  L YPEVSDL   R C+SL  NDTWA
Sbjct: 365 ASSVDRSFPTLVTLGINWTTTGQAMFTGSETGFIKLAYPEVSDLKYPRDCKSLRENDTWA 424

Query: 421 AGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGM 480
           AG+VVLCF S        +T+  ++K G LGLIVA+NPT+ +    ++FPCVQ+S DI M
Sbjct: 425 AGSVVLCFTSSFKEGYIEDTAYWIRKAGALGLIVAENPTRTLYSCSDDFPCVQVSYDIAM 484

Query: 481 QILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILA 540
           +IL YIR+T++P+V+I  + T  G+P+ST +AYFSSRGP+SVA A+LKPDIAAPGV IL+
Sbjct: 485 KILYYIRTTKSPKVRISPTKTHDGRPVSTNLAYFSSRGPSSVASAVLKPDIAAPGVNILS 544

Query: 541 AVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYG 600
           AV PSDPK +N++AF+SGTSMATPHVS IVALLKTLH  WSPAAIKSAIVTTAW+SDP G
Sbjct: 545 AVSPSDPKTKNAFAFMSGTSMATPHVSGIVALLKTLHPDWSPAAIKSAIVTTAWNSDPSG 604

Query: 601 AVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGIT 660
             IFAEG+ M  A PFD+GGGIVN N+A DPGLVYDMG  DYI Y C+ GY  SAIS + 
Sbjct: 605 EPIFAEGETMKLADPFDYGGGIVNFNRAADPGLVYDMGSTDYIHYLCSMGYEISAISQLR 664

Query: 661 KRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAI 720
           +   SCP +RPSILD+N+PSITIPSL    S  RTVTNVG + S YKA ++ P GI I++
Sbjct: 665 EHPTSCPTKRPSILDLNLPSITIPSLRRATSFRRTVTNVGHVESRYKAVVDPPQGIRISV 724

Query: 721 KPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           +PR+L FN   ++I+F VT+SS+HRVTTGY FGSLTW DGVHSVR P+SVRT I
Sbjct: 725 RPRLLIFNSTTRAITFKVTVSSSHRVTTGYYFGSLTWTDGVHSVRSPISVRTEI 777

BLAST of Carg15037 vs. TrEMBL
Match: tr|A0A2P5DFB5|A0A2P5DFB5_PARAD (Subtilase OS=Parasponia andersonii OX=3476 GN=PanSBT24 PE=4 SV=1)

HSP 1 Score: 950.3 bits (2455), Expect = 2.7e-273
Identity = 476/774 (61.50%), Postives = 590/774 (76.23%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60
           MG+    K +S T+  V +S+ + ++    L      KSNVYI++MGQK H++ +LL K 
Sbjct: 5   MGASLEMKKNSATLLFVFVSLISIINEHYLL-TAADPKSNVYIIHMGQKQHEDVKLLKKT 64

Query: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120
           HH +LA+VLGS+E S  +++YSYK+GFSGFAAKLT  QAQ ISELP VV VIPN+L+K+Q
Sbjct: 65  HHELLATVLGSKEESKRAMIYSYKHGFSGFAAKLTKTQAQTISELPGVVLVIPNQLYKVQ 124

Query: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180
           TTRSWDYLQL  R      +KS+ G GAIIG+LDTGIWPESE F D+GLGP+PSRWKG+C
Sbjct: 125 TTRSWDYLQLYSRDQTHFEQKSKSGDGAIIGLLDTGIWPESEAFSDKGLGPIPSRWKGVC 184

Query: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240
           ESG+ +  AK C+RKLIGARYF+K L A YG P N +E  DY SPRD SGHGTH S++A+
Sbjct: 185 ESGQNYDGAKHCNRKLIGARYFIKALVAEYGKPYNATEAGDYLSPRDSSGHGTHTSTIAA 244

Query: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQV-NGGVCADIDILKAIDQAIYDGVDV 300
           G  + N SY+GL VGTVRGGA  +R+AMYKVCW +  GG+CA  DILKA D+A+ DGVDV
Sbjct: 245 GSSMANRSYNGLGVGTVRGGATQARVAMYKVCWSLPAGGMCAGADILKAFDEAVRDGVDV 304

Query: 301 LSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVA 360
           +S+S+    PS+ +VD R+ IA+GAFHAVA+G+VVV +AGN+GP+  +V N  PW+LTVA
Sbjct: 305 VSVSIAADVPSFHEVDERSAIAVGAFHAVAQGVVVVCSAGNAGPSPQTVQNASPWILTVA 364

Query: 361 ASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWA 420
           ASSVDRSF   +TLG+NWTT GQ MF+G  T F  L YPEV DL   R C+SL  NDTW 
Sbjct: 365 ASSVDRSFPTLVTLGSNWTTTGQAMFTGSETGFIKLAYPEVPDLKYPRDCKSLRENDTWT 424

Query: 421 AGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGM 480
           AG+VVLCF S        +T+  ++K G LGLIVA+NPT+++     +FPCVQ+S DI M
Sbjct: 425 AGSVVLCFTSSFKEGYIEDTAYWIQKAGALGLIVAENPTRSLYSCSADFPCVQVSYDIAM 484

Query: 481 QILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILA 540
           QIL YIR+T++P+V+I  + T  G+P+ST +AYFSSRGP+SVAPA+LKPDIAAPGV IL+
Sbjct: 485 QILYYIRTTKSPKVRISPTKTHDGRPVSTNLAYFSSRGPSSVAPAVLKPDIAAPGVNILS 544

Query: 541 AVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYG 600
           AV PSDPK +N++AF+SGTSMATPHVS IVALLKTLH  WSPAAIKSAIVTTAW+SDP G
Sbjct: 545 AVSPSDPKTKNAFAFMSGTSMATPHVSGIVALLKTLHPDWSPAAIKSAIVTTAWNSDPSG 604

Query: 601 AVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGIT 660
             IFAEG+ M  A PFD+GGGIVN N+A DPGLVYDMG  DYI Y C+ GY  SAIS + 
Sbjct: 605 EPIFAEGETMKLADPFDYGGGIVNFNRAADPGLVYDMGSTDYIHYLCSMGYETSAISQLR 664

Query: 661 KRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAI 720
           +   SCP +RPSILD+N+PSITIPSL    S  RTVTNVG ++S Y+A +  P GI I++
Sbjct: 665 EHPTSCPSKRPSILDLNLPSITIPSLRRTTSFRRTVTNVGHVDSRYEAVVNPPQGIGISV 724

Query: 721 KPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 774
           +PR+L FN   ++I+F VT+SS+HRVTTGY FGSLTW DGVHSVR P+SVRT I
Sbjct: 725 RPRLLIFNSTTRAITFKVTVSSSHRVTTGYYFGSLTWTDGVHSVRSPISVRTEI 777

BLAST of Carg15037 vs. TrEMBL
Match: tr|A0A061G9L3|A0A061G9L3_THECC (Subtilase family protein, putative OS=Theobroma cacao OX=3641 GN=TCM_047016 PE=4 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 3.0e-272
Identity = 480/737 (65.13%), Postives = 564/737 (76.53%), Query Frame = 0

Query: 37  AKSNVYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTM 96
           + SNVYIVYMG++ H + E L + HH +L++VL S+E S +S+VYSYK+GFSGFAAK+T 
Sbjct: 36  SNSNVYIVYMGKRQHPDVERLTRTHHEMLSTVLVSEETSKESMVYSYKHGFSGFAAKMTE 95

Query: 97  AQAQMISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTG 156
           AQAQ +S+LP VV V  N  +K+QTTRSWDYL LS   P++LL KS+MG+G IIG+LDTG
Sbjct: 96  AQAQKLSKLPGVVHVTRNGFYKLQTTRSWDYLGLSTNSPSNLLNKSKMGNGVIIGLLDTG 155

Query: 157 IWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNN 216
           IWPESEVF DEGLGP+PSRWKG+CESGELF  AKAC+RKLIGARYF++GL+A YG P N 
Sbjct: 156 IWPESEVFSDEGLGPIPSRWKGVCESGELFDGAKACNRKLIGARYFIRGLQAEYGQPYNT 215

Query: 217 SEFQDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVN 276
           S   DY SPRD SGHGTH SS+A G FV NVSY+GL  GTVRGGAP +R+AMYKVCWQ+ 
Sbjct: 216 SANNDYLSPRDPSGHGTHTSSIAGGSFVANVSYYGLGFGTVRGGAPGARLAMYKVCWQLY 275

Query: 277 GGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVG 336
           GGVC+D D+LK  D+AI+DGVDVLS+SL    P YSDVD R  I IG+FHAVAKGI VV 
Sbjct: 276 GGVCSDADVLKGFDEAIHDGVDVLSVSLVADIPLYSDVDQRGSIPIGSFHAVAKGITVVC 335

Query: 337 AAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLV 396
           AAGN+GP A +V N  PW+LTVAAS+VDRSF   I LGNN T MGQ MF+G+ T F  LV
Sbjct: 336 AAGNAGPRAETVQNTAPWILTVAASTVDRSFPTPIMLGNNQTIMGQAMFTGEDTVFATLV 395

Query: 397 YPEVSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKN 456
           YPEVSDL   R CESLS ND W AG VVLCF S+       +   SVK+ GGLG+IVA+ 
Sbjct: 396 YPEVSDLMVPRNCESLSSNDDWMAGKVVLCFVSEYNMSLLDDGIWSVKEAGGLGVIVART 455

Query: 457 PTKAVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSR 516
           P+  +  +   FPCVQ++ + G QIL YIRST NPQV++  S T VG+PLST+VAYFSSR
Sbjct: 456 PSDYLYSYATRFPCVQVTYETGTQILYYIRSTSNPQVRLSPSRTHVGKPLSTSVAYFSSR 515

Query: 517 GPNSVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLH 576
           GP+S APAILKPDIAAPGV ILAA PP  P +  ++AF SGTSMATPHVS IVALLK+L+
Sbjct: 516 GPSSNAPAILKPDIAAPGVKILAASPPDRPTN-GAFAFRSGTSMATPHVSGIVALLKSLY 575

Query: 577 SHWSPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDM 636
             WSPAAIKSAIVTTA S+D  G  IFAEG+P   A PFDFGGGIVNPN A DPGLVYDM
Sbjct: 576 PDWSPAAIKSAIVTTALSADQSGGPIFAEGEPSKLADPFDFGGGIVNPNGAADPGLVYDM 635

Query: 637 GMADYIQYFCAKGYNDSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVT 696
              DY QY CA GYNDSAI  +T+  I CP ++PS+LD+N+PSITIPSL  P  LTRTVT
Sbjct: 636 NTEDYGQYLCAMGYNDSAIFQLTQHPIVCPSKQPSVLDVNLPSITIPSLRKPTILTRTVT 695

Query: 697 NVGAINSSYKAAIEAPPGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTW 756
           NVG +NS YKA +E   GI IA++P IL F+ K K+I+FTV ISS H V  GY FGSLTW
Sbjct: 696 NVGPVNSKYKANVEFASGINIAVRPEILIFSSKTKTITFTVMISSAHNVNAGYYFGSLTW 755

Query: 757 LDGVHSVRIPVSVRTNI 774
            DG H VR P+SVRT +
Sbjct: 756 TDGGHVVRSPISVRTEV 771

BLAST of Carg15037 vs. TrEMBL
Match: tr|A0A2I4ESG7|A0A2I4ESG7_9ROSI (subtilisin-like protease SBT3.6 isoform X2 OS=Juglans regia OX=51240 GN=LOC108992284 PE=4 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 1.7e-270
Identity = 484/783 (61.81%), Postives = 591/783 (75.48%), Query Frame = 0

Query: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLH---IRVAAKSNVYIVYMGQKPHDNEELL 60
           M S    K+ +TT+    +S+F  L  L   H       A  NVYIVYMG+    + +LL
Sbjct: 1   MKSIRRCKNRNTTL----LSLFMFLLFLYEQHSIPTLAEANRNVYIVYMGKGQQRDPKLL 60

Query: 61  VKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISEL-----PSVVEVI 120
           ++ H  +L  VLGS+E S +++VYSYK+GF+GFAAKLT  QAQ+IS L      +VV+VI
Sbjct: 61  LETHLEILTGVLGSKEASAEAMVYSYKHGFTGFAAKLTGTQAQIISGLLYLYSITVVQVI 120

Query: 121 PNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPV 180
           PNR +K+QTTRSWDYLQL+   P ++L KS+MG G IIGV+DTGIWPESEVF DEGLGP+
Sbjct: 121 PNRFYKLQTTRSWDYLQLNSYSPKNILHKSQMGDGVIIGVIDTGIWPESEVFRDEGLGPI 180

Query: 181 PSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHG 240
           PSRWKG CESGELF   K C+RKLIGARYF+KGL A YG   N +++QDY SPRD  GHG
Sbjct: 181 PSRWKGACESGELFHARKTCNRKLIGARYFIKGLHAEYGKSFNITKYQDYLSPRDSFGHG 240

Query: 241 THVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQA 300
           TH S+ A G+F  NVSY+GL  GTVRGGAP +R+A+YKVCW++ G  CA  DILKA D+A
Sbjct: 241 THTSATAGGYFKANVSYNGLGFGTVRGGAPLARLAIYKVCWKLYGVKCASADILKAFDKA 300

Query: 301 IYDGVDVLSLSLGPSFPSYSDVD-MRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNI 360
           I+DGVDV+SLSLG + P +SD+D   NGI++GAFHA   GI VV AAGN+GP+A ++ N 
Sbjct: 301 IHDGVDVISLSLGFALPLFSDIDTTHNGISVGAFHAAENGITVVCAAGNAGPSALTIQNT 360

Query: 361 EPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACES 420
            PW+++VAAS++DRSF   I+LGNNWTTMGQ MF+G+ T F NL+YPEVS+    R C S
Sbjct: 361 APWVISVAASTIDRSFPTRISLGNNWTTMGQAMFTGQGTGFTNLLYPEVSEFHTPRYCGS 420

Query: 421 LSLNDTWAAGNVVLCFA-SDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPC 480
           LS NDTW+AGNVVLCF    D      + + SVK++G LGLI+A+NP        +NFPC
Sbjct: 421 LSSNDTWSAGNVVLCFTLGSDNRSAVGDAARSVKEIGCLGLIIAENPKSFPYSCDDNFPC 480

Query: 481 VQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDI 540
           V++S DIGMQILNYIRSTRNPQV I  S T VG+P+ST VAYFSSRGP+SVAPAILKPD+
Sbjct: 481 VRVSYDIGMQILNYIRSTRNPQVMIRPSKTHVGRPISTRVAYFSSRGPSSVAPAILKPDV 540

Query: 541 AAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVT 600
           AAPGV ILAAVPP   +   ++ F SGTSMA PHVS IVALLK+LHS WSPA IKSAIVT
Sbjct: 541 AAPGVNILAAVPPRS-RPGIAFEFDSGTSMAAPHVSGIVALLKSLHSDWSPAIIKSAIVT 600

Query: 601 TAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGY 660
           TAW++DPYG  I+AEG+PM  A  FD+GGGIVN N+A DPGLVYDMG ADYIQY CA GY
Sbjct: 601 TAWTTDPYGEPIYAEGEPMKLADAFDYGGGIVNGNRAADPGLVYDMGTADYIQYLCAMGY 660

Query: 661 NDSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIE 720
            +S IS +T     CP ++PSIL++N PSITIP+L +  ++TRTVTNVGA+ S Y A +E
Sbjct: 661 KNSDISQVTGNPAFCPMKQPSILNLNFPSITIPTLRNSTTITRTVTNVGAVYSRYVALVE 720

Query: 721 APPGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVR 774
            P GI+IA+KP IL FN  +K+ISFTVT+SS+H+VTTGY FGSLTW DGVHSVR P+SVR
Sbjct: 721 PPLGISIAVKPDILIFNSIIKAISFTVTVSSSHKVTTGYSFGSLTWSDGVHSVRSPISVR 778