Carg15006 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg15006
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	gibberellin 2-beta-dioxygenase-like
Location	Cucurbita_argyrosperma_scaffold_071 : 589079 .. 590181 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGTTCTGTCTCAGCCTGCATTGGATTTAGCCAAATATTCTCTTCTCCGAGCAACATGTAAACCCGCCGTCGCCGCCGCCGCGGCGGATATTCCGGCAATCGACCTCGCAGACCCTGACGCCAAGTCCCACATCGTCAAGGCTTGCGAAGAAATTGGCTTCTTTAAGGTGGTAAGCCATCGAGTTCCCATCGAATTGATGACCAAACTGGAAGATGAAGCCCTCCGTTTTTTCAGTCTTTCTCAGGCTGAGAAAGATAAAGCTCGTCCCCCTTACCCTTTGGGCTATGGCTCCAAAAATATCGGCCCTAATGGCGATAAGGGGTGGATTGAATATCTCCTCCTAAATGCTAATCCTCTCCCTCTTTTCGCCCCAAACTCCGAATCTTTAATGTAAGTTACACAAAATTGAGCACTCTGTTTCTTTGAATTTAACCCAAATTTGTAAAATAAAATATATTTTTCATAGAATTTGAAAATTTATGCTAATTTTTGTGTAGCGCTGCCGTGAAGGAGTACGTGACGGCGGTGAAGAAGCTGAGCTGCGAAGTGGCGGAGCTAATAGCAGAGGGATTAAAAATCGAGCCACGAAACACAATAAGCAAGCTTCTAAATGAGGAGAAAAGCGATTGCTGTTTCAGAGTCAACCGGTATCCGCCGTGTCCGGAGCTCCAAGCTGTTGGTGGAAAAAGCATGATCGGGTTCGGGGAACACACAGACCCACAAATCATATCACTGTTAAGATCAAACAACTCGACCGGTCTACAAATCTGCCTGAGAGACGGCGCGTGGGTTTCAGTGCCACCAGACTCAGCGGCGTTCTTCGTGAACGTCGGCGACGCGCTGCAGGTGATGAGCAACGGCAGATTCCGAAGCGTGAAGCACAAAGTGGTGGCGGATTGTAACATCGAGAGGGTTTCAATGATTTACTTTGGAGGGCCGGCGTTGAGTGAAAAGATTGCGCCATTGCCACAGATTTTGGGAGACGGAGAAGAGAGCTTGTACAAGGAATTCTACTGGAGGGAATATAAAACGGCGGCGTACAAGACTAGATTGGCTGATTACAGGCTTGGCCCCTTTGAGAAGAATCAAAATTCGTAG

mRNA sequence

ATGGTTGTTCTGTCTCAGCCTGCATTGGATTTAGCCAAATATTCTCTTCTCCGAGCAACATGTAAACCCGCCGTCGCCGCCGCCGCGGCGGATATTCCGGCAATCGACCTCGCAGACCCTGACGCCAAGTCCCACATCGTCAAGGCTTGCGAAGAAATTGGCTTCTTTAAGGTGGTAAGCCATCGAGTTCCCATCGAATTGATGACCAAACTGGAAGATGAAGCCCTCCGTTTTTTCAGTCTTTCTCAGGCTGAGAAAGATAAAGCTCGTCCCCCTTACCCTTTGGGCTATGGCTCCAAAAATATCGGCCCTAATGGCGATAAGGGGTGGATTGAATATCTCCTCCTAAATGCTAATCCTCTCCCTCTTTTCGCCCCAAACTCCGAATCTTTAATCGCTGCCGTGAAGGAGTACGTGACGGCGGTGAAGAAGCTGAGCTGCGAAGTGGCGGAGCTAATAGCAGAGGGATTAAAAATCGAGCCACGAAACACAATAAGCAAGCTTCTAAATGAGGAGAAAAGCGATTGCTGTTTCAGAGTCAACCGGTATCCGCCGTGTCCGGAGCTCCAAGCTGTTGGTGGAAAAAGCATGATCGGGTTCGGGGAACACACAGACCCACAAATCATATCACTGTTAAGATCAAACAACTCGACCGGTCTACAAATCTGCCTGAGAGACGGCGCGTGGGTTTCAGTGCCACCAGACTCAGCGGCGTTCTTCGTGAACGTCGGCGACGCGCTGCAGGTGATGAGCAACGGCAGATTCCGAAGCGTGAAGCACAAAGTGGTGGCGGATTGTAACATCGAGAGGGTTTCAATGATTTACTTTGGAGGGCCGGCGTTGAGTGAAAAGATTGCGCCATTGCCACAGATTTTGGGAGACGGAGAAGAGAGCTTGTACAAGGAATTCTACTGGAGGGAATATAAAACGGCGGCGTACAAGACTAGATTGGCTGATTACAGGCTTGGCCCCTTTGAGAAGAATCAAAATTCGTAG

Coding sequence (CDS)

ATGGTTGTTCTGTCTCAGCCTGCATTGGATTTAGCCAAATATTCTCTTCTCCGAGCAACATGTAAACCCGCCGTCGCCGCCGCCGCGGCGGATATTCCGGCAATCGACCTCGCAGACCCTGACGCCAAGTCCCACATCGTCAAGGCTTGCGAAGAAATTGGCTTCTTTAAGGTGGTAAGCCATCGAGTTCCCATCGAATTGATGACCAAACTGGAAGATGAAGCCCTCCGTTTTTTCAGTCTTTCTCAGGCTGAGAAAGATAAAGCTCGTCCCCCTTACCCTTTGGGCTATGGCTCCAAAAATATCGGCCCTAATGGCGATAAGGGGTGGATTGAATATCTCCTCCTAAATGCTAATCCTCTCCCTCTTTTCGCCCCAAACTCCGAATCTTTAATCGCTGCCGTGAAGGAGTACGTGACGGCGGTGAAGAAGCTGAGCTGCGAAGTGGCGGAGCTAATAGCAGAGGGATTAAAAATCGAGCCACGAAACACAATAAGCAAGCTTCTAAATGAGGAGAAAAGCGATTGCTGTTTCAGAGTCAACCGGTATCCGCCGTGTCCGGAGCTCCAAGCTGTTGGTGGAAAAAGCATGATCGGGTTCGGGGAACACACAGACCCACAAATCATATCACTGTTAAGATCAAACAACTCGACCGGTCTACAAATCTGCCTGAGAGACGGCGCGTGGGTTTCAGTGCCACCAGACTCAGCGGCGTTCTTCGTGAACGTCGGCGACGCGCTGCAGGTGATGAGCAACGGCAGATTCCGAAGCGTGAAGCACAAAGTGGTGGCGGATTGTAACATCGAGAGGGTTTCAATGATTTACTTTGGAGGGCCGGCGTTGAGTGAAAAGATTGCGCCATTGCCACAGATTTTGGGAGACGGAGAAGAGAGCTTGTACAAGGAATTCTACTGGAGGGAATATAAAACGGCGGCGTACAAGACTAGATTGGCTGATTACAGGCTTGGCCCCTTTGAGAAGAATCAAAATTCGTAG

Protein sequence

MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVSHRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANPLPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAAFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLYKEFYWREYKTAAYKTRLADYRLGPFEKNQNS

Homology

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BLAST of Carg15006 vs. NCBI nr
Match: XP_022926667.1 (gibberellin 2-beta-dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 664.1 bits (1712), Expect = 2.5e-187
Identity = 328/331 (99.09%), Postives = 330/331 (99.70%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS
Sbjct: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120
           HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEY LLNANP
Sbjct: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYFLLNANP 120

Query: 121 LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LPLFAPNS+SLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV
Sbjct: 121 LPLFAPNSKSLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAAFF 240
           NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPD+AAFF
Sbjct: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAFF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY
Sbjct: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNS 332
           KEFYWREYKTAAYKTRLADYRLGPFEKNQNS
Sbjct: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNS 331

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BLAST of Carg15006 vs. NCBI nr
Match: XP_023003444.1 (gibberellin 2-beta-dioxygenase-like [Cucurbita maxima])

HSP 1 Score: 637.1 bits (1642), Expect = 3.3e-179
Identity = 318/330 (96.36%), Postives = 322/330 (97.58%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPALDLAKYSLLRATCKP  AAAAA+IPAIDLADPDAKSHIVKACEEIGFFKVVS
Sbjct: 1   MVVLSQPALDLAKYSLLRATCKP--AAAAANIPAIDLADPDAKSHIVKACEEIGFFKVVS 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120
           H+VPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP
Sbjct: 61  HQVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120

Query: 121 LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LPLF PNSESLIAAVKEYVTAVKKLSCEVAELIAE LKIEPRNTISKLLNEEKSDCCFRV
Sbjct: 121 LPLFVPNSESLIAAVKEYVTAVKKLSCEVAELIAEELKIEPRNTISKLLNEEKSDCCFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAAFF 240
           NRYPPCPELQAV GKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDG WVSVPPD+AAFF
Sbjct: 181 NRYPPCPELQAVDGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGEWVSVPPDAAAFF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVMSNGRFRSVKHKVVAD N ERVSMIYFGGPALSEKIAPLPQILGDGEESLY
Sbjct: 241 VNVGDALQVMSNGRFRSVKHKVVADSNNERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQN 331
           KEFYWREYKTAAYKT+LADYRLGPFEKNQN
Sbjct: 301 KEFYWREYKTAAYKTKLADYRLGPFEKNQN 328

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BLAST of Carg15006 vs. NCBI nr
Match: XP_023518407.1 (gibberellin 2-beta-dioxygenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 622.1 bits (1603), Expect = 1.1e-174
Identity = 311/331 (93.96%), Postives = 313/331 (94.56%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPALDLAKYSLLRATCKP    AAADIPAIDLADPDAKSHIVKACEEIGFFKVVS
Sbjct: 1   MVVLSQPALDLAKYSLLRATCKP----AAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120
           HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP
Sbjct: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120

Query: 121 LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV
Sbjct: 121 LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAAFF 240
           NRYPPCPEL+AVGGKS IGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPD+ AFF
Sbjct: 181 NRYPPCPELEAVGGKSTIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDATAFF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVMSNGRFRSVKHKVVADCN ERVSMIYFGGPALSEKIAPLPQILGDGEESLY
Sbjct: 241 VNVGDALQVMSNGRFRSVKHKVVADCNNERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNS 332
           KEFYW           LADYRLGPFEKNQNS
Sbjct: 301 KEFYWXXXXXXXXXXXLADYRLGPFEKNQNS 327

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BLAST of Carg15006 vs. NCBI nr
Match: AUB30295.1 (gibberellin 2-beta-dioxygenase 8-like protein [Luffa aegyptiaca])

HSP 1 Score: 568.2 bits (1463), Expect = 1.9e-158
Identity = 280/333 (84.08%), Postives = 304/333 (91.29%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPAL L +YSLLR TCKP   A+  +IP IDL+DP+A+SHIV+ACEE GFFK+V+
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKP--TASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120
           HRVPIELMTKLE+EAL FF LSQ+EKDKARPP PLGYG KNIG NGDKGWIEYLLL ANP
Sbjct: 61  HRVPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANP 120

Query: 121 LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LP+F+ NSES +AA KEYVTAVKKLSCEVAELIAEGLKIEPRN ISKLLN+EKSDCCFRV
Sbjct: 121 LPIFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRV 180

Query: 181 NRYPPCPELQAVG--GKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAA 240
           N YPPCP+LQA+G  G++MIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPD+AA
Sbjct: 181 NHYPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAA 240

Query: 241 FFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEES 300
            FVNVGD+LQVMSNGRF+SVKHKVVAD N ERVSMIYFGGP LSEKIAPLP++LGDGEES
Sbjct: 241 IFVNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEES 300

Query: 301 LYKEFYWREYKTAAYKTRLADYRLGPFEKNQNS 332
           LYKEF W EYKTAAYKTRLADYRLGPFEKNQNS
Sbjct: 301 LYKEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 331

[Close]

BLAST of Carg15006 vs. NCBI nr
Match: CBL59329.1 (Gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 542.7 bits (1397), Expect = 8.5e-151
Identity = 266/331 (80.36%), Postives = 296/331 (89.43%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPALDL KYSLLR+T KP  AA+AADIP IDL+DP+AKSH+VKACEE GFFK+V+
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKP--AASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120
           HRVP+ELMTKLEDE+L FF LSQ+EKDKARPP PLGYGSKNIG NGDKGWIEYLLLNANP
Sbjct: 61  HRVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANP 120

Query: 121 LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LPLF+  S+S ++A  EYVTAVKKLS EV ELI EGLKIEP+N IS+L+N+EK+DC FRV
Sbjct: 121 LPLFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAAFF 240
           N YPPCPE+QAV G++MIGFGEHTDPQIISLLRSNNSTGLQIC RDG WVSVPPD+ AFF
Sbjct: 181 NHYPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVM+NGRF+SVKHKVVAD + ERVSMIYFGGP LSEKI PL Q+L +GEESLY
Sbjct: 241 VNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNS 332
           KEF W EYKTAA+KTRLADYRLG FEK+Q S
Sbjct: 301 KEFTWWEYKTAAFKTRLADYRLGAFEKSQKS 329

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BLAST of Carg15006 vs. TAIR10
Match: AT1G30040.1 (gibberellin 2-oxidase)

HSP 1 Score: 369.4 bits (947), Expect = 2.3e-102
Identity = 189/335 (56.42%), Postives = 241/335 (71.94%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVL QP + L  +  L  T KP     +  IP ++LADP+AK+ IVKACEE GFFKVV+
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120
           H V  ELMT+LE EA+ FF L Q+ K++A PP P GYG+K IGPNGD GWIEYLLLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 LPLFAPNSESLIAAV--------KEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEE 180
             L +P + ++                      S +V E++AE L IEPR+T+SK+L +E
Sbjct: 121 -QLSSPKTSAVFRQTPXXXXXXXXXXXXXXXXXSYKVLEMVAEELGIEPRDTLSKMLRDE 180

Query: 181 KSDCCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSV 240
           KSD C R+N YP   E      K  +GFGEHTDPQIIS+LRSNN+ GLQIC++DG+WV+V
Sbjct: 181 KSDSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAV 240

Query: 241 PPDSAAFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQIL 300
           PPD ++FF+NVGDALQVM+NGRF+SVKH+V+AD    R+SMIYFGGP LS+KIAPLP ++
Sbjct: 241 PPDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLV 300

Query: 301 GDGEESLYKEFYWREYKTAAYKTRLADYRLGPFEK 328
            + ++ LYKEF W +YK++AYK++L DYRLG FEK
Sbjct: 301 PEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEK 331

[Close]

BLAST of Carg15006 vs. TAIR10
Match: AT2G34555.1 (gibberellin 2-oxidase 3)

HSP 1 Score: 346.7 bits (888), Expect = 1.6e-95
Identity = 178/335 (53.13%), Postives = 229/335 (68.36%), Query Frame = 0


Query: 1   MVVLSQPA-LDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVV 60
           MV++ QPA  D   Y  +   CKP        IP IDL D DAK+ IVKACEE GFFKV+
Sbjct: 1   MVIVLQPASFDSNLY--VNPKCKPRPVL----IPVIDLTDSDAKTQIVKACEEFGFFKVI 60

Query: 61  SHRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNAN 120
           +H V  +L+T+LE EA+ FF+L  + KDKA PP P GYG+K IGPNGD GW+EY+LLNAN
Sbjct: 61  NHGVRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNAN 120

Query: 121 -------PLPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEE 180
                     +F         AV+EY+  +K++S +  E++ E LKIEP+  +S+L+  +
Sbjct: 121 LCLESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVK 180

Query: 181 KSDCCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSV 240
           +SD C R+N Y   PE +    K  IGFGEHTDPQ+ISLLRSN++ GLQIC++DG WV V
Sbjct: 181 ESDSCLRMNHY---PEKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDV 240

Query: 241 PPDSAAFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQIL 300
            PD ++FFV VGD LQVM+NGRF+SVKH+VV +    R+SMIYF GP LSEKIAPL  ++
Sbjct: 241 TPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLV 300

Query: 301 GDGEESLYKEFYWREYKTAAYKTRLADYRLGPFEK 328
              ++ LY EF W +YK +AYKT+L DYRLG FEK
Sbjct: 301 PKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEK 326

[Close]

BLAST of Carg15006 vs. TAIR10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1)

HSP 1 Score: 343.2 bits (879), Expect = 1.8e-94
Identity = 164/305 (53.77%), Postives = 228/305 (74.75%), Query Frame = 0


Query: 32  IPAIDLADPDAKSHIVKACEEIGFFKVVSHRVPIELMTKLEDEALRFFSLSQAEKDKARP 91
           IP ID++DP++K  +VKACE+ GFFKV++H V  EL++ LE E + FFSL ++EK +   
Sbjct: 18  IPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQV-A 77

Query: 92  PYPLGYGSKNIGPNGDKGWIEYLLLNAN------PL-PLFAPNSESLIAAVKEYVTAVKK 151
            YP GYG+  IG NGD GW+EYLL+NAN      PL P    +  +   A++EY T+V+K
Sbjct: 78  GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTSVRK 137

Query: 152 LSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRVNRYPPCP--ELQAVGGKSMIGFGE 211
           ++ +V E I +GL I+PRNT+SKL++++ +D   R+N YPPCP    +  GGK++IGFGE
Sbjct: 138 MTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIGFGE 197

Query: 212 HTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAAFFVNVGDALQVMSNGRFRSVKHKV 271
           HTDPQIIS+LRSNN++GLQI L DG+W+SVPPD  +FF NVGD+LQVM+NGRF+SV+H+V
Sbjct: 198 HTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVRHRV 257

Query: 272 VADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLYKEFYWREYKTAAYKTRLADYRL 328
           +A+C   RVSMIYF GP+L+++IAPL  ++ + +E LY+EF W EYK + Y +RL+D RL
Sbjct: 258 LANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSDNRL 317

[Close]

BLAST of Carg15006 vs. TAIR10
Match: AT1G02400.1 (gibberellin 2-oxidase 6)

HSP 1 Score: 254.6 bits (649), Expect = 8.4e-68
Identity = 136/322 (42.24%), Postives = 195/322 (60.56%), Query Frame = 0


Query: 17  LRATCKPAVAAAAADIPAIDLADPD---AKSHIVKACEEIGFFKVVSHRVPIELMTKLED 76
           L+ T K  +++   + P ID +  D       IVKACE  GFFKV++H V  E++ + E 
Sbjct: 9   LQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEH 68

Query: 77  EALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANPLPLFAPNSESL-- 136
           E   FF+  +++K +A P  P GYG KNIG NGD G +EYLLL+ANP  + A  SE++  
Sbjct: 69  EGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAV-ADKSETISH 128

Query: 137 ------IAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRVNRYPP 196
                  +A  +Y+  V+ L+CE+ +L  E L  +  + +S+L+ + +SD   R+N YPP
Sbjct: 129 DDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPP 188

Query: 197 CPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAAFFVNVGD 256
            P   A+ G   IGFGEH+DPQI+++LRSN+  GL+IC RDG W+ +P D   FFV VGD
Sbjct: 189 AP--YALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGD 248

Query: 257 ALQVMSNGRFRSVKHKVVAD-CNIERVSMIYFGGPALSEKIAPLPQILGDGEESLYKEFY 316
            LQ ++NGRF SV+H+V+A+     R+S +YF  P L  KI+PLP+++       Y  F 
Sbjct: 249 CLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSFT 308

Query: 317 WREYKTAAYKTRLADYRLGPFE 327
           W +YK A Y  RL   RL  F+
Sbjct: 309 WGDYKKATYSLRLDVPRLEFFK 327

[Close]

BLAST of Carg15006 vs. TAIR10
Match: AT1G47990.1 (gibberellin 2-oxidase 4)

HSP 1 Score: 244.2 bits (622), Expect = 1.1e-64
Identity = 138/312 (44.23%), Postives = 184/312 (58.97%), Query Frame = 0


Query: 25  VAAAAADIPAIDLAD--PDAKSHIVKACEEIGFFKVVSHRVPIELMTKLEDEALRFFSLS 84
           + A   DIP ID++         IVKACE +GFFKV++H V    ++++E E++ FF+  
Sbjct: 8   IVAVDQDIPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFFAKP 67

Query: 85  QAEKDKARP-PYPLGYGSKNIGPNGDKGWIEYLLLNANPLPLFAPNSESLIAAVKEYVTA 144
             EK   RP   P  YG ++IG NGD G +EYLL + N  P F  +  S  +AV  Y+ A
Sbjct: 68  AHEKKSVRPVNQPFRYGFRDIGLNGDSGEVEYLLFHTND-PAFR-SQLSFSSAVNCYIEA 127

Query: 145 VKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRVNRYPPCPELQAVGGKS----- 204
           VK+L+ E+ +L AEGL + P ++ S+L++   SD   RVN YPP  +       S     
Sbjct: 128 VKQLAREILDLTAEGLHVPP-HSFSRLISSVDSDSVLRVNHYPPSDQFFGEANLSDQSVS 187

Query: 205 --MIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAAFFVNVGDALQVMSNGR 264
              +GFGEHTDPQI+++LRSN   GLQ+   DG WVSV PD +AF VNVGD LQVM+NGR
Sbjct: 188 LTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNVGDLLQVMTNGR 247

Query: 265 FRSVKHKVVADCNIERVSMIYFGGPALSEKIAPL-PQILGDGEESLYKEFYWREYKTAAY 324
           F SV+H+ +      R+S  YF GP L  KI PL   ++   +  LY+ F W EYK  AY
Sbjct: 248 FISVRHRALTYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLYQTFTWGEYKKRAY 307

Query: 325 KTRLADYRLGPF 326
             RL D RL  F
Sbjct: 308 SLRLEDSRLDMF 316

[Close]

BLAST of Carg15006 vs. Swiss-Prot
Match: sp|Q9XG83|G2OX_PHACN (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 8.7e-115
Identity = 209/336 (62.20%), Postives = 254/336 (75.60%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAA-ADIPAIDLADPDAKSHIVKACEEIGFFKVV 60
           MVVLSQPAL+  ++ LL    KP  +      IP +DL  PDAK+ IV AC + GFFK+V
Sbjct: 1   MVVLSQPALN--QFFLL----KPFKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLV 60

Query: 61  SHRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNAN 120
           +H VP+ELM  LE+EALRFF  SQ+EKD+A PP P GYGSK IGPNGD GW+EYLLLN N
Sbjct: 61  NHGVPLELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTN 120

Query: 121 P-------LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEE 180
           P       L +F  N     A V+ Y+TAVK +   V EL+AEGL I  RNT+S+LL +E
Sbjct: 121 PDVISPKSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDE 180

Query: 181 KSDCCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSV 240
           KSD CFR+N YPPCPE+QA+  ++++GFGEHTDPQIIS+LRSN+++GLQICL DG WVSV
Sbjct: 181 KSDSCFRLNHYPPCPEVQAL-NRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSV 240

Query: 241 PPDSAAFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQIL 300
           PPD  +FF+NVGDALQVM+NGRF+SVKH+V+AD    R+SMIYFGGPALSE IAPLP ++
Sbjct: 241 PPDQTSFFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVM 300

Query: 301 GDGEESLYKEFYWREYKTAAYKTRLADYRLGPFEKN 329
             GEE LYKEF W EYK AAY +RLAD RL PF+K+
Sbjct: 301 LKGEECLYKEFTWCEYKKAAYTSRLADNRLAPFQKS 329

[Close]

BLAST of Carg15006 vs. Swiss-Prot
Match: sp|Q9SQ80|G2OX1_PEA (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 7.7e-103
Identity = 182/330 (55.15%), Postives = 251/330 (76.06%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MV+LS+P  +  +Y+ +R          ++ IP +DL+ PDAK+ IVKACE+ GFFKV++
Sbjct: 1   MVLLSKPTSE--QYTYVRNNMP---ITFSSSIPLVDLSKPDAKTLIVKACEDFGFFKVIN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120
           H +P++ +++LE EA +FFSL Q EK+KA P  P GYG+K IG NGD GWIEYLLL  N 
Sbjct: 61  HGIPLDAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTNQ 120

Query: 121 ---LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCC 180
                L+  + +     +K+Y  A++ ++CE+ +L+AEGLKI+P+N  SKL+ +++SDC 
Sbjct: 121 DHNFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCL 180

Query: 181 FRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSA 240
           FRVN YP CPEL A+ G+++IGFGEHTDPQIIS+LRSNN++G QI LRDG+W+SVPPD +
Sbjct: 181 FRVNHYPACPEL-AINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHS 240

Query: 241 AFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEE 300
           +FF+NVGD+LQVM+NGRF+SV+H+V+A+    R+SMIYF GP LSEKIAPLP ++  G+E
Sbjct: 241 SFFINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPSLM-KGKE 300

Query: 301 SLYKEFYWREYKTAAYKTRLADYRLGPFEK 328
           SLYKEF W EYK++ Y +RLAD RLG +E+
Sbjct: 301 SLYKEFTWFEYKSSTYGSRLADNRLGNYER 323

[Close]

BLAST of Carg15006 vs. Swiss-Prot
Match: sp|Q9XFR9|G2OX2_ARATH (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 4.2e-101
Identity = 189/335 (56.42%), Postives = 241/335 (71.94%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVL QP + L  +  L  T KP     +  IP ++LADP+AK+ IVKACEE GFFKVV+
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120
           H V  ELMT+LE EA+ FF L Q+ K++A PP P GYG+K IGPNGD GWIEYLLLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 LPLFAPNSESLIAAV--------KEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEE 180
             L +P + ++                      S +V E++AE L IEPR+T+SK+L +E
Sbjct: 121 -QLSSPKTSAVFRQTPXXXXXXXXXXXXXXXXXSYKVLEMVAEELGIEPRDTLSKMLRDE 180

Query: 181 KSDCCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSV 240
           KSD C R+N YP   E      K  +GFGEHTDPQIIS+LRSNN+ GLQIC++DG+WV+V
Sbjct: 181 KSDSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAV 240

Query: 241 PPDSAAFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQIL 300
           PPD ++FF+NVGDALQVM+NGRF+SVKH+V+AD    R+SMIYFGGP LS+KIAPLP ++
Sbjct: 241 PPDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLV 300

Query: 301 GDGEESLYKEFYWREYKTAAYKTRLADYRLGPFEK 328
            + ++ LYKEF W +YK++AYK++L DYRLG FEK
Sbjct: 301 PEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEK 331

[Close]

BLAST of Carg15006 vs. Swiss-Prot
Match: sp|O64692|G2OX3_ARATH (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.9e-94
Identity = 178/335 (53.13%), Postives = 229/335 (68.36%), Query Frame = 0


Query: 1   MVVLSQPA-LDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVV 60
           MV++ QPA  D   Y  +   CKP        IP IDL D DAK+ IVKACEE GFFKV+
Sbjct: 1   MVIVLQPASFDSNLY--VNPKCKPRPVL----IPVIDLTDSDAKTQIVKACEEFGFFKVI 60

Query: 61  SHRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNAN 120
           +H V  +L+T+LE EA+ FF+L  + KDKA PP P GYG+K IGPNGD GW+EY+LLNAN
Sbjct: 61  NHGVRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNAN 120

Query: 121 -------PLPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEE 180
                     +F         AV+EY+  +K++S +  E++ E LKIEP+  +S+L+  +
Sbjct: 121 LCLESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVK 180

Query: 181 KSDCCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSV 240
           +SD C R+N Y   PE +    K  IGFGEHTDPQ+ISLLRSN++ GLQIC++DG WV V
Sbjct: 181 ESDSCLRMNHY---PEKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDV 240

Query: 241 PPDSAAFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQIL 300
            PD ++FFV VGD LQVM+NGRF+SVKH+VV +    R+SMIYF GP LSEKIAPL  ++
Sbjct: 241 TPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLV 300

Query: 301 GDGEESLYKEFYWREYKTAAYKTRLADYRLGPFEK 328
              ++ LY EF W +YK +AYKT+L DYRLG FEK
Sbjct: 301 PKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEK 326

[Close]

BLAST of Carg15006 vs. Swiss-Prot
Match: sp|Q8S0S6|G2OX3_ORYSJ (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.9e-94
Identity = 182/332 (54.82%), Postives = 231/332 (69.58%), Query Frame = 0


Query: 1   MVVLS-QPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVV 60
           MVVL+  PA+D     LLR+   P      + +P +DL  P A   +V ACE  GFFKVV
Sbjct: 1   MVVLAGPPAVD--HIPLLRS---PDPGDVFSGVPVVDLGSPGAARAVVDACERYGFFKVV 60

Query: 61  SHRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNAN 120
           +H V  + M K E EA+RFFS +Q +KD++ P YP GYGSK IG NGD GW+EYLLL  +
Sbjct: 61  NHGVATDTMDKAESEAVRFFSQTQPDKDRSGPAYPFGYGSKRIGFNGDMGWLEYLLLALD 120

Query: 121 PLPL----FAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSD 180
              L      P+     AA+ EY++ V+K++  V E ++EGL I   + +S L+  E SD
Sbjct: 121 DASLADACTVPSCAVFRAALNEYISGVRKVAVRVMEAMSEGLGIAQADALSALVTAEGSD 180

Query: 181 CCFRVNRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPD 240
             FRVN YPPC  LQ + G S+ GFGEHTDPQ++S+LRSN ++GLQI LRDG WVSVP D
Sbjct: 181 QVFRVNHYPPCRALQGL-GCSVTGFGEHTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSD 240

Query: 241 SAAFFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDG 300
             +FFVNVGD+LQV++NGRF+SVKH+VVA+    RVS IYFGGP L+++IAPLPQ+LG+G
Sbjct: 241 RDSFFVNVGDSLQVLTNGRFKSVKHRVVANSLKSRVSFIYFGGPPLAQRIAPLPQLLGEG 300

Query: 301 EESLYKEFYWREYKTAAYKTRLADYRLGPFEK 328
           E+SLYKEF W EYK AAYK+RL D RL  FEK
Sbjct: 301 EQSLYKEFTWDEYKKAAYKSRLGDNRLAQFEK 326

[Close]

BLAST of Carg15006 vs. TrEMBL
Match: tr|A0A2H4V3T7|A0A2H4V3T7_LUFAE (Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 1.2e-158
Identity = 280/333 (84.08%), Postives = 304/333 (91.29%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPAL L +YSLLR TCKP   A+  +IP IDL+DP+A+SHIV+ACEE GFFK+V+
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKP--TASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120
           HRVPIELMTKLE+EAL FF LSQ+EKDKARPP PLGYG KNIG NGDKGWIEYLLL ANP
Sbjct: 61  HRVPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANP 120

Query: 121 LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LP+F+ NSES +AA KEYVTAVKKLSCEVAELIAEGLKIEPRN ISKLLN+EKSDCCFRV
Sbjct: 121 LPIFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRV 180

Query: 181 NRYPPCPELQAVG--GKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAA 240
           N YPPCP+LQA+G  G++MIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPD+AA
Sbjct: 181 NHYPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAA 240

Query: 241 FFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEES 300
            FVNVGD+LQVMSNGRF+SVKHKVVAD N ERVSMIYFGGP LSEKIAPLP++LGDGEES
Sbjct: 241 IFVNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEES 300

Query: 301 LYKEFYWREYKTAAYKTRLADYRLGPFEKNQNS 332
           LYKEF W EYKTAAYKTRLADYRLGPFEKNQNS
Sbjct: 301 LYKEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 331

[Close]

BLAST of Carg15006 vs. TrEMBL
Match: tr|I0J1J0|I0J1J0_CUCMA (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox2 PE=2 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 5.6e-151
Identity = 266/331 (80.36%), Postives = 296/331 (89.43%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQPALDL KYSLLR+T KP  AA+AADIP IDL+DP+AKSH+VKACEE GFFK+V+
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKP--AASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120
           HRVP+ELMTKLEDE+L FF LSQ+EKDKARPP PLGYGSKNIG NGDKGWIEYLLLNANP
Sbjct: 61  HRVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANP 120

Query: 121 LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LPLF+  S+S ++A  EYVTAVKKLS EV ELI EGLKIEP+N IS+L+N+EK+DC FRV
Sbjct: 121 LPLFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAAFF 240
           N YPPCPE+QAV G++MIGFGEHTDPQIISLLRSNNSTGLQIC RDG WVSVPPD+ AFF
Sbjct: 181 NHYPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVM+NGRF+SVKHKVVAD + ERVSMIYFGGP LSEKI PL Q+L +GEESLY
Sbjct: 241 VNVGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKNQNS 332
           KEF W EYKTAA+KTRLADYRLG FEK+Q S
Sbjct: 301 KEFTWWEYKTAAFKTRLADYRLGAFEKSQKS 329

[Close]

BLAST of Carg15006 vs. TrEMBL
Match: tr|A0A1S3AZ81|A0A1S3AZ81_CUCME (gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103484366 PE=3 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 2.6e-148
Identity = 260/328 (79.27%), Postives = 290/328 (88.41%), Query Frame = 0


Query: 1   MVVLSQPALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVVS 60
           MVVLSQP LDL +YSLLR+TCKP   A+ ADIP IDL+DP+AK HIVKACEE GFFK+V+
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKP--TASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVN 60

Query: 61  HRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNANP 120
           HRVPIELMTKLEDE+L FF LS++EKDKARPP+PLGYGSKNIG NGDKGWIEYLL+NANP
Sbjct: 61  HRVPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANP 120

Query: 121 LPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFRV 180
           LP+F   S   + A  EYVTAVKKLSCEV ELIAEGLK+E RN ISKLLN+EK+DCCFRV
Sbjct: 121 LPIF---SHDFLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRV 180

Query: 181 NRYPPCPELQAVGGKSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAAFF 240
           N YPPCPE+Q + G++MIGFGEHTDPQIIS+LRSNNSTGLQICLRDGAWVSVPPD+ A F
Sbjct: 181 NHYPPCPEMQGLSGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALF 240

Query: 241 VNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEESLY 300
           VNVGDALQVM+NGRF+SVKHKVV D N ERVSMIYFGGP LSEKI PLP++L DGEESLY
Sbjct: 241 VNVGDALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLY 300

Query: 301 KEFYWREYKTAAYKTRLADYRLGPFEKN 329
           KEF W EYKTAAYK++LADYRLG FEK+
Sbjct: 301 KEFTWWEYKTAAYKSKLADYRLGAFEKS 323

[Close]

BLAST of Carg15006 vs. TrEMBL
Match: tr|S0BE28|S0BE28_CUCSA (GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox4 PE=2 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 4.8e-142
Identity = 255/330 (77.27%), Postives = 285/330 (86.36%), Query Frame = 0


Query: 1   MVVLSQ-PALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVV 60
           MVVLSQ P LD  +YSLLR+TCKP   A+ ADIP IDL+DP+AK HIVKACEE GFFK+V
Sbjct: 1   MVVLSQPPTLDFNQYSLLRSTCKP--TASFADIPEIDLSDPNAKFHIVKACEEFGFFKLV 60

Query: 61  SHRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNAN 120
           +H VP+ELMTKLEDE+L FF LS++EKDKAR P+PLGYGSKNIG NGDKGWIEYLLLNAN
Sbjct: 61  NHGVPVELMTKLEDESLCFFKLSKSEKDKARTPHPLGYGSKNIGSNGDKGWIEYLLLNAN 120

Query: 121 PLPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFR 180
           PLP+F   S   + A  EYVTAVKKLSCEV ELIAEGLK+E RN ISKLL +EK+DCCFR
Sbjct: 121 PLPIF---SHDFLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLKDEKADCCFR 180

Query: 181 VNRYPPCPELQAVGG-KSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAA 240
           VN YPPCPE+Q + G  +MIGFGEHTDPQI+S+LRSNNSTGLQICLRDGAWVSVP D+AA
Sbjct: 181 VNHYPPCPEMQGLSGLNNMIGFGEHTDPQILSILRSNNSTGLQICLRDGAWVSVPADAAA 240

Query: 241 FFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEES 300
           FFVNVGD LQVM+NGRF+SVKHKVV D N ERVSMIYFGGP LSEKI PLP++L DGEES
Sbjct: 241 FFVNVGDVLQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPEVLKDGEES 300

Query: 301 LYKEFYWREYKTAAYKTRLADYRLGPFEKN 329
           LYKEF W EYKTAAYK++LADYRLG FEK+
Sbjct: 301 LYKEFTWWEYKTAAYKSKLADYRLGAFEKS 325

[Close]

BLAST of Carg15006 vs. TrEMBL
Match: tr|A0A0A0KIK7|A0A0A0KIK7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G523390 PE=3 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 7.1e-138
Identity = 251/330 (76.06%), Postives = 281/330 (85.15%), Query Frame = 0


Query: 1   MVVLSQ-PALDLAKYSLLRATCKPAVAAAAADIPAIDLADPDAKSHIVKACEEIGFFKVV 60
           MVVLSQ P LD  +YSLLR+TCKP   A+ ADIP IDL+DP+AK HIVKACEE GFFK+V
Sbjct: 1   MVVLSQPPTLDFNQYSLLRSTCKP--TASFADIPEIDLSDPNAKFHIVKACEEFGFFKLV 60

Query: 61  SHRVPIELMTKLEDEALRFFSLSQAEKDKARPPYPLGYGSKNIGPNGDKGWIEYLLLNAN 120
           +H VP+ELMTKLEDE+L FF LS++EKDKAR P+PLGYGS     NGDKGWIEYLLLNAN
Sbjct: 61  NHGVPVELMTKLEDESLCFFKLSKSEKDKARTPHPLGYGS-----NGDKGWIEYLLLNAN 120

Query: 121 PLPLFAPNSESLIAAVKEYVTAVKKLSCEVAELIAEGLKIEPRNTISKLLNEEKSDCCFR 180
           PLP+F   S   + A  EYVTAVKKLSCEV ELIAEGLK+E RN ISKLL +EK+DCCFR
Sbjct: 121 PLPIF---SHDFLCAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLKDEKADCCFR 180

Query: 181 VNRYPPCPELQAVGG-KSMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDSAA 240
           VN YPPCPE+Q + G  +MIGFGEHTDPQI+S+LRSNNSTGLQICLRDGAWVSVP D+AA
Sbjct: 181 VNHYPPCPEMQGLSGLNNMIGFGEHTDPQILSILRSNNSTGLQICLRDGAWVSVPADAAA 240

Query: 241 FFVNVGDALQVMSNGRFRSVKHKVVADCNIERVSMIYFGGPALSEKIAPLPQILGDGEES 300
           FFVNVGD LQVM+NGRF+SVKHKVV D N ERVSMIYFGGP LSEKI PLP++L DGEES
Sbjct: 241 FFVNVGDVLQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPEVLKDGEES 300

Query: 301 LYKEFYWREYKTAAYKTRLADYRLGPFEKN 329
           LYKEF W EYKTAAYK++LADYRLG FEK+
Sbjct: 301 LYKEFTWWEYKTAAYKSKLADYRLGAFEKS 320

The following BLAST results are available for this feature:

BLAST of Carg15006 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022926667.1	2.5e-187	99.09	gibberellin 2-beta-dioxygenase-like [Cucurbita moschata]	[more]
XP_023003444.1	3.3e-179	96.36	gibberellin 2-beta-dioxygenase-like [Cucurbita maxima]	[more]
XP_023518407.1	1.1e-174	93.96	gibberellin 2-beta-dioxygenase-like [Cucurbita pepo subsp. pepo]	[more]
AUB30295.1	1.9e-158	84.08	gibberellin 2-beta-dioxygenase 8-like protein [Luffa aegyptiaca]	[more]
CBL59329.1	8.5e-151	80.36	Gibberellin 2-oxidase [Cucurbita maxima]	[more]

BLAST of Carg15006 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G30040.1	2.3e-102	56.42	gibberellin 2-oxidase	[more]
AT2G34555.1	1.6e-95	53.13	gibberellin 2-oxidase 3	[more]
AT1G78440.1	1.8e-94	53.77	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]
AT1G02400.1	8.4e-68	42.24	gibberellin 2-oxidase 6	[more]
AT1G47990.1	1.1e-64	44.23	gibberellin 2-oxidase 4	[more]

BLAST of Carg15006 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9XG83\|G2OX_PHACN	8.7e-115	62.20	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]
sp\|Q9SQ80\|G2OX1_PEA	7.7e-103	55.15	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
sp\|Q9XFR9\|G2OX2_ARATH	4.2e-101	56.42	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
sp\|O64692\|G2OX3_ARATH	2.9e-94	53.13	Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ...	[more]
sp\|Q8S0S6\|G2OX3_ORYSJ	2.9e-94	54.82	Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]

BLAST of Carg15006 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A2H4V3T7\|A0A2H4V3T7_LUFAE	1.2e-158	84.08	Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 S...	[more]
tr\|I0J1J0\|I0J1J0_CUCMA	5.6e-151	80.36	Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox2 PE=2 SV=1	[more]
tr\|A0A1S3AZ81\|A0A1S3AZ81_CUCME	2.6e-148	79.27	gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103484366 PE=3...	[more]
tr\|S0BE28\|S0BE28_CUCSA	4.8e-142	77.27	GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox4 PE=2 SV=1	[more]
tr\|A0A0A0KIK7\|A0A0A0KIK7_CUCSA	7.1e-138	76.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G523390 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027443	IPNS-like
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR026992	DIOX_N

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg15006-RA	Carg15006-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 135..155
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 2..327
None	No IPR available	PANTHER	PTHR10209:SF387	GIBBERELLIN 2-BETA-DIOXYGENASE 2-RELATED	coord: 2..327
None	No IPR available	SUPERFAMILY	SSF51197	Clavaminate synthase-like	coord: 27..319
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 32..117 e-value: 4.3E-13 score: 50.0
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 175..277 e-value: 9.9E-25 score: 86.9
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 173..279 score: 11.619
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 12..318 e-value: 1.9E-75 score: 256.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg15006	Cla009774	Watermelon (97103) v1	carwmB0730
Carg15006	Csa6G523390	Cucumber (Chinese Long) v2	carcuB0748
Carg15006	MELO3C007132	Melon (DHL92) v3.5.1	carmeB0725
Carg15006	ClCG01G024630	Watermelon (Charleston Gray)	carwcgB0707
Carg15006	CSPI06G36290	Wild cucumber (PI 183967)	carcpiB0764
Carg15006	Cucsa.047740	Cucumber (Gy14) v1	carcgyB0822
Carg15006	CmaCh07G001050	Cucurbita maxima (Rimu)	carcmaB1024
Carg15006	CmaCh03G013990	Cucurbita maxima (Rimu)	carcmaB1023
Carg15006	CmoCh07G001060	Cucurbita moschata (Rifu)	carcmoB0996
Carg15006	CmoCh03G013920	Cucurbita moschata (Rifu)	carcmoB0995
Carg15006	Lsi01G001480	Bottle gourd (USVL1VR-Ls)	carlsiB669
Carg15006	Cp4.1LG10g05910	Cucurbita pepo (Zucchini)	carcpeB1028
Carg15006	Cp4.1LG19g06140	Cucurbita pepo (Zucchini)	carcpeB1029
Carg15006	CsGy6G035650	Cucumber (Gy14) v2	carcgybB0726
Carg15006	MELO3C007132.2	Melon (DHL92) v3.6.1	carmedB0736
Carg15006	CsaV3_6G051750	Cucumber (Chinese Long) v3	carcucB0767
Carg15006	Cla97C01G024580	Watermelon (97103) v2	carwmbB0764
Carg15006	Cla97C03G058120	Watermelon (97103) v2	carwmbB0765
Carg15006	Bhi03G000076	Wax gourd	carwgoB0863
Carg15006	Bhi08G001697	Wax gourd	carwgoB0864

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg15006	Carg19269	Silver-seed gourd	carcarB328

The following block(s) are covering this gene:


Gene	Organism	Block
Carg15006	Silver-seed gourd	carcarB437
Carg15006	Cucurbita maxima (Rimu)	carcmaB1021
Carg15006	Cucurbita maxima (Rimu)	carcmaB1022
Carg15006	Cucurbita moschata (Rifu)	carcmoB0993
Carg15006	Cucurbita moschata (Rifu)	carcmoB0994
Carg15006	Cucurbita pepo (Zucchini)	carcpeB1027
Carg15006	Cucurbita pepo (Zucchini)	carcpeB1030
Carg15006	Bottle gourd (USVL1VR-Ls)	carlsiB670
Carg15006	Melon (DHL92) v3.5.1	carmeB0724
Carg15006	Watermelon (Charleston Gray)	carwcgB0708
Carg15006	Watermelon (97103) v1	carwmB0729