Carg14852 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg14852
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	tRNA (Guanine-N(7)-)-methyltransferase
Location	Cucurbita_argyrosperma_scaffold_079 : 312558 .. 315834 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGCAGCGCCATTCTTGAGAGCCCAACGGACGCGGCAAAATGGCTTCGTCCCTTTGCTTCCAGTTACACACTTGCTCCCAGCTTCGTTGCATTGCTGCTGCGAAGGCGGCAGCGGCAGCGGCGCCGTCGAGCGTCGCAACAGCAAACCGAACGTTATCTCTGTTAGTAAAACGACCATTTCTTCAATCCTTCTGTTTACCATCGGGCTCTTCCGCCGAGCTTTGCGAGCGGCAACAACTCCGGAGTCCGGAACTTGTGGCGTTGGAATATGCGGACCTTAATCTCTCTCACAAGATCTTCGAGGTTTGTTTTCTGTTCGTGAATTTTGCCCTTGATTTCTGAAATCGTTGGCGAGTAATTGAAAATTGAATGTGGTGACTAGAATTGATGTGCATTTGACTTGGTTTTGGGAATTGCAGGATTCAGGTCACGTCAGAATCAGGCAGCATGTTAATCCACTTAGCTCTTCTTTCTCTGTATGTAATTTGAGTTTTGAAAGTGGATTTGGTTTCTCTCTGATGAGAAATTTTAGGAGCTGTGGCTTTGAAATTTTAGCTTAGTGTTGCTTAATTGAAGGAAAAGCTCTATCTCTTTTACCTTTCAGGTCCCTACACCAGCACCTAACTGGGATGAAGTCTTCAAGGACCCCACGTTGCCCCTCATGGTCGATATAGGAAGTGGTGAGTGAGCTGATGCATTCCCTGTTATATACCTCTTACATATCTGTTTTCTTCATAATAGCTACTTTCTTAGTACATACTTCGCTTTCACGAGATAATATTTGATTCTGTGTAGCATTTGGCTGAATCCATATTCCTAAAATCGTTTTTAACTTGCTTTTGATTTCTAAGTCAATCCTCAGTCGTTGAAAATCAGTTTTGATGACATGAAAATTGTAACAGCCCAATCCCACCCCCACCGATAGTAGATACTGTCCTCTTTGGGCTTTCCCTTTCGGGCTTCCCCTCAAGGTTTTTAAAATGCGTCGGGTAATGAGAAGTTTCCACACCCTTATAAAAAATGTTTCGTTCTCCTCCCCAACCGATGTGGGACCTCACAATCCATCCCTTTTCGGGGCACAGCATCCTTGCCGCACACCACCTTGTGTCCACCCCCTTCGGGGTCTAACATCCTTGCTGGTACACCACCTTGTGTCCACCCCCCTTCGGGGCTCAGTCTCCTCGTTGGCACATCGCCTGATGTCTGGCTCTGATACCATTTGTAACAACCCAAGTCCACCGCTAGCAAATATTATCCTCTTTGAGCTTTTTCCCTTTCGGGCTTTCCCTCAATATTCTTAAAATGCGTATGCTAGGGAGAGGTTTCCACGCCCTTATAAAGAATAATTCGTTCTCCTCCCTAACCGACGTGGGATCTTACAAAAATAATGTTTAAAAGTGTATTATTTCATTTTGAATGGTTAAACTCATGTTTAGGAGTGATTTTAACCATCACTGATATTGCTTATATTATAATTAGAATCTTACCCATAATCCTCCTGCTTTGGCCAAATAGAGGATAGCCATGAACAAGTGTTTGATGCTACTGCTTGATTTTGAAAACAAGTTCAAAACATAATTTAGAGATCCTACCATTTGCTCAATCTGTTTGGTGATGTTCATTGAACCAGCATGAAGAAGGAAATATTATCCTAAAGCATCATATTGATTACTGCATTCAAAGATCATCTTTACCGTGCACTGTAAATCAAAATTTATCTCGATATGCGTTATAAAACAGCTCTAATCATGTAATCAATACGTTACATGTCGAGCAAAATGCTCCAAGCAATAGCAGCAACGAGCTCGCTAGTAAACATTAAGTTTAATACAATGTAGTCGCCTAAGTCGCTCTGGAGAATTTACACAGGAAAGAATTCTGTTGGTAATGATATTAAGGTTTCAAATGCTAATTGCCTATAGTATCGTATTGTATTCACATGCAAGAAAATAATTTATCAGATTAAGACATGCATGCTGTAATTGTTAGTCGTGAGTCCCGCGTTGGCTAATTTAGGGAATGATCATGGGTTTATAAGTAAAGGAATACATTTCCATTGGTATGAGGCGTTTTGGGAAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGGATAATATCATACCATTGTTGGGAGTCGTGATTCCTAACAATAATGATTTACGTAACATCTTTACTTTCATGCATTTTTAAGAATTCATGAGACAATTTAATGCCTTTGATTTCCAATCAAGAATCTAACAGAACAAGTATTCCTATTCCCAGGTAGTGGTAGATTTCTCTTGTGGCTTGCAAAAAAACATCTTGATTCAAGAAATTTCTTGGGTCTCGAAATACGACAGAAAGTACGGGACCGAAAATTTTCACTTTTGATGCACCCTTAGTTGGTCTGAACTATTTTATCCTCGTTCTATCGAGGCATTATATCGTGCTTTGATTCCTTACGTGCTCTTGCAGTTGGTCAAGCGTGCCGATGTCTGGGTCAAAGAGTTGGGATTTAACAATGTGTAAGGACCTTAACTTCATAGCAGGTAGTGAAAAACAAGATCACCTTTTTGCTATTGGTACATTAGTAATGAACTTCTTCTTGAACTTGAATGCAGACATTTCATGTTTGCAAATGCTACAATTTCTTTTAAACAGATAATATCTTCTTATCCTGGACCCCTGATGCTGGTTTCCATATTGGTTAGTGCAACCCCCCCTCCCCGAACGATTCAACCCGAACTTTCTCATTGAATATCAACGAAGATAGAACTCACTCGTGCTTAATCTTGTCACAGTGCCCAGATCCCCATTTCAAGAAACGACATCATAAGCGACGGGTTTTGCAGAAGCCATTAGTAGATTCTATAATAGACAGTTTAATGCCAGGAGGGCAGGTATATGATCTGCCAATCCATGACATTCAATGGTTTTTTTTGCCTGTTTGATGAGTCCGATGCTGGAAATTTTGTTGTATATGATTCAACAGGTGTTCATACAATCCGACGTGCTCGAAGTGGCAGTTGACATGAGACTTTGGTTCGATTCTGTTCCCGATGTTTTGAAACACGTCGACGTGCTCGACCAGAGTCTTTTGTGTGATAAAGAGGGATGGTTATTGAAGAATCCAATGGGAATAAGGACTGAAAGAGAAGTTCACGCTGAATCCGAAGGTGCGAACATTTACAGAAGAATGTATCAGAAGTATGTTTGCATTTGAGTCATCATTCACTGTTTTTTTTTTTTTTTGTTCTTTTCTAGAAATATATTTATTGCATCTTTATAATT

mRNA sequence

CGGCAGCGCCATTCTTGAGAGCCCAACGGACGCGGCAAAATGGCTTCGTCCCTTTGCTTCCAGTTACACACTTGCTCCCAGCTTCGTTGCATTGCTGCTGCGAAGGCGGCAGCGGCAGCGGCGCCGTCGAGCGTCGCAACAGCAAACCGAACGTTATCTCTGTTAGTAAAACGACCATTTCTTCAATCCTTCTGTTTACCATCGGGCTCTTCCGCCGAGCTTTGCGAGCGGCAACAACTCCGGAGTCCGGAACTTGTGGCGTTGGAATATGCGGACCTTAATCTCTCTCACAAGATCTTCGAGGATTCAGGTCACGTCAGAATCAGGCAGCATGTTAATCCACTTAGCTCTTCTTTCTCTGTCCCTACACCAGCACCTAACTGGGATGAAGTCTTCAAGGACCCCACGTTGCCCCTCATGGTCGATATAGGAAGTGGTAGTGGTAGATTTCTCTTGTGGCTTGCAAAAAAACATCTTGATTCAAGAAATTTCTTGGGTCTCGAAATACGACAGAAATTGGTCAAGCGTGCCGATGTCTGGGTCAAAGAGTTGGGATTTAACAATGTACATTTCATGTTTGCAAATGCTACAATTTCTTTTAAACAGATAATATCTTCTTATCCTGGACCCCTGATGCTGGTTTCCATATTGTGCCCAGATCCCCATTTCAAGAAACGACATCATAAGCGACGGGTTTTGCAGAAGCCATTAGTAGATTCTATAATAGACAGTTTAATGCCAGGAGGGCAGGTGTTCATACAATCCGACGTGCTCGAAGTGGCAGTTGACATGAGACTTTGGTTCGATTCTGTTCCCGATGTTTTGAAACACGTCGACGTGCTCGACCAGAGTCTTTTGTGTGATAAAGAGGGATGGTTATTGAAGAATCCAATGGGAATAAGGACTGAAAGAGAAGTTCACGCTGAATCCGAAGGTGCGAACATTTACAGAAGAATGTATCAGAAGTATGTTTGCATTTGAGTCATCATTCACTGTTTTTTTTTTTTTTTGTTCTTTTCTAGAAATATATTTATTGCATCTTTATAATT

Coding sequence (CDS)

ATGGCTTCGTCCCTTTGCTTCCAGTTACACACTTGCTCCCAGCTTCGTTGCATTGCTGCTGCGAAGGCGGCAGCGGCAGCGGCGCCGTCGAGCGTCGCAACAGCAAACCGAACGTTATCTCTGTTAGTAAAACGACCATTTCTTCAATCCTTCTGTTTACCATCGGGCTCTTCCGCCGAGCTTTGCGAGCGGCAACAACTCCGGAGTCCGGAACTTGTGGCGTTGGAATATGCGGACCTTAATCTCTCTCACAAGATCTTCGAGGATTCAGGTCACGTCAGAATCAGGCAGCATGTTAATCCACTTAGCTCTTCTTTCTCTGTCCCTACACCAGCACCTAACTGGGATGAAGTCTTCAAGGACCCCACGTTGCCCCTCATGGTCGATATAGGAAGTGGTAGTGGTAGATTTCTCTTGTGGCTTGCAAAAAAACATCTTGATTCAAGAAATTTCTTGGGTCTCGAAATACGACAGAAATTGGTCAAGCGTGCCGATGTCTGGGTCAAAGAGTTGGGATTTAACAATGTACATTTCATGTTTGCAAATGCTACAATTTCTTTTAAACAGATAATATCTTCTTATCCTGGACCCCTGATGCTGGTTTCCATATTGTGCCCAGATCCCCATTTCAAGAAACGACATCATAAGCGACGGGTTTTGCAGAAGCCATTAGTAGATTCTATAATAGACAGTTTAATGCCAGGAGGGCAGGTGTTCATACAATCCGACGTGCTCGAAGTGGCAGTTGACATGAGACTTTGGTTCGATTCTGTTCCCGATGTTTTGAAACACGTCGACGTGCTCGACCAGAGTCTTTTGTGTGATAAAGAGGGATGGTTATTGAAGAATCCAATGGGAATAAGGACTGAAAGAGAAGTTCACGCTGAATCCGAAGGTGCGAACATTTACAGAAGAATGTATCAGAAGTATGTTTGCATTTGA

Protein sequence

MASSLCFQLHTCSQLRCIAAAKAAAAAAPSSVATANRTLSLLVKRPFLQSFCLPSGSSAELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGANIYRRMYQKYVCI

Homology

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BLAST of Carg14852 vs. NCBI nr
Match: XP_023527130.1 (uncharacterized protein LOC111790461 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 609.8 bits (1571), Expect = 5.3e-171
Identity = 299/313 (95.53%), Postives = 302/313 (96.49%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAXXXXXXXXXXXVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLCFQLHTCSQLRCIAA           VATANRTLSLL+KRPFLQSFCLPSGSSAE
Sbjct: 1   MASSLCFQLHTCSQLRCIAAAKAAAVAAPSSVATANRTLSLLLKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDK+GWLLKNPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKDGWLLKNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKYVCI 314
           NIYRRMYQKYVCI
Sbjct: 301 NIYRRMYQKYVCI 313

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BLAST of Carg14852 vs. NCBI nr
Match: XP_022924717.1 (uncharacterized protein LOC111432133 [Cucurbita moschata])

HSP 1 Score: 606.3 bits (1562), Expect = 5.9e-170
Identity = 309/313 (98.72%), Postives = 312/313 (99.68%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAXXXXXXXXXXXVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLCFQLHTCSQLRCIAAXXXXXXXXXXXVATANRTLSLLVKRPFLQSFCLPSGSSAE
Sbjct: 1   MASSLCFQLHTCSQLRCIAAXXXXXXXXXXXVATANRTLSLLVKRPFLQSFCLPSGSSAE 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVAVDMRLWFDSVP+VLKHVDV DQSLLCDK+GWLLKNPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHVDVHDQSLLCDKDGWLLKNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKYVCI 314
           NIYRRMYQKYVCI
Sbjct: 301 NIYRRMYQKYVCI 313

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BLAST of Carg14852 vs. NCBI nr
Match: XP_022980361.1 (uncharacterized protein LOC111479752 [Cucurbita maxima])

HSP 1 Score: 602.8 bits (1553), Expect = 6.5e-169
Identity = 307/313 (98.08%), Postives = 310/313 (99.04%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAXXXXXXXXXXXVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLCFQLHTCSQLRCIAAXXXXXXXXXXXVATANRTLSLLVKRPFL SFCLPSGSSAE
Sbjct: 1   MASSLCFQLHTCSQLRCIAAXXXXXXXXXXXVATANRTLSLLVKRPFLHSFCLPSGSSAE 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK
Sbjct: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSL PGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLKPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVAVDMRLWFDSVP+VLKHVDVLDQSLLCDK+GWLL NPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAVDMRLWFDSVPNVLKHVDVLDQSLLCDKDGWLLNNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQKYVCI 314
           NIYRRMYQKYVCI
Sbjct: 301 NIYRRMYQKYVCI 313

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BLAST of Carg14852 vs. NCBI nr
Match: XP_022146903.1 (uncharacterized protein LOC111015991 [Momordica charantia])

HSP 1 Score: 522.7 bits (1345), Expect = 8.6e-145
Identity = 258/312 (82.69%), Postives = 277/312 (88.78%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAXXXXXXXXXXXVATANRTLS--LLVKRPFLQSFCLPSGSS 60
           MA SLCFQLHTCSQL CIAA           VATANRTLS  L ++RPFL SFC+  G S
Sbjct: 1   MAPSLCFQLHTCSQLGCIAA---AKAAAPSSVATANRTLSPFLRLRRPFLHSFCVRLGPS 60

Query: 61  AELCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEV 120
           AE+ E Q LRSPELVALEYADLNL H+IFEDSGHVRIRQHVNPLSSSF+VP PAPNW+EV
Sbjct: 61  AEVFEPQHLRSPELVALEYADLNLPHRIFEDSGHVRIRQHVNPLSSSFAVPAPAPNWNEV 120

Query: 121 FKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHF 180
           FKDPTLPLMVDIGSGSGRFLLWLAK+HLD RNFLGLEIR KLVKRAD+WVKELG +NVHF
Sbjct: 121 FKDPTLPLMVDIGSGSGRFLLWLAKRHLDMRNFLGLEIRHKLVKRADIWVKELGLSNVHF 180

Query: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQV 240
           MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRV+QKPLV+SI+DSL PGGQV
Sbjct: 181 MFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVVQKPLVESIVDSLTPGGQV 240

Query: 241 FIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESE 300
            IQSDVLEVAVDMRLWFDS  DVLKH+DVLD S+LCD++GWLL NPMGIRTERE+HAESE
Sbjct: 241 LIQSDVLEVAVDMRLWFDSEADVLKHIDVLDPSVLCDEDGWLLNNPMGIRTEREIHAESE 300

Query: 301 GANIYRRMYQKY 311
           GA IYRRMYQK+
Sbjct: 301 GAKIYRRMYQKF 309

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BLAST of Carg14852 vs. NCBI nr
Match: XP_008438199.1 (PREDICTED: tRNA (guanine-N(7)-)-methyltransferase [Cucumis melo])

HSP 1 Score: 521.2 bits (1341), Expect = 2.5e-144
Identity = 256/309 (82.85%), Postives = 276/309 (89.32%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAXXXXXXXXXXXVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLC  L TCS LRC+AA           VATAN+ LS   KRPFL++F LPSGSSA+
Sbjct: 1   MASSLCLHLQTCSHLRCLAA---AKPAAPSVVATANKILS---KRPFLRNFSLPSGSSAD 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRS ELVALEYADLNLSHK+ ED GHVRIRQHVNPLSSSFSVPTPAPNWDEVF+
Sbjct: 61  LCERQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFR 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DPTLPLMVDIGSGSGRFLLWLAK HL+SRNFLGLEIRQKLVKRA  WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKSHLESRNFLGLEIRQKLVKRAGFWVKELGFSNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPL+LVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLLLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVA DMRLWFDS  D+L+H+D +D+S+LCDK+GWLL NPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAADMRLWFDSEVDILEHIDKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQK 310
           NIYRRMY K
Sbjct: 301 NIYRRMYSK 303

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BLAST of Carg14852 vs. TAIR10
Match: AT5G17660.1 (tRNA (guanine-N-7) methyltransferase)

HSP 1 Score: 369.0 bits (946), Expect = 2.9e-102
Identity = 177/256 (69.14%), Postives = 214/256 (83.59%), Query Frame = 0


Query: 55  SGSSAELC-ERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAP 114
           S +S  +C + + LRS ELV LEYA+LNL+HKI E+ GHVRIRQHVNPLSSSFS P P P
Sbjct: 55  SSASTSVCVDFKDLRSNELVELEYAELNLNHKISEEVGHVRIRQHVNPLSSSFSKPAPVP 114

Query: 115 NWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGF 174
            WDEV+KDP+LPLMVDIGSGSGRFLLW A K+++SRN+LGLEIRQKLVKRA+ WV ELG 
Sbjct: 115 VWDEVYKDPSLPLMVDIGSGSGRFLLWQANKNVESRNYLGLEIRQKLVKRANFWVNELGL 174

Query: 175 NNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLM 234
           +NVHF+FANA +SF+ +ISSYPGPL +VSILCPDPHFKKRH KRRV+QKPLV+SI+ +L 
Sbjct: 175 SNVHFIFANAMVSFEHLISSYPGPLEIVSILCPDPHFKKRHQKRRVVQKPLVNSILQNLK 234

Query: 235 PGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREV 294
           PGG++F+QSDVL+VA DMR   D   +VL+H+D +D      ++GWL +NPMGIRTERE+
Sbjct: 235 PGGKIFVQSDVLDVAQDMRDQLDEESNVLQHMDTVD-----TEDGWLTENPMGIRTEREI 294

Query: 295 HAESEGANIYRRMYQK 310
           HAE EGA IYRR+YQK
Sbjct: 295 HAEFEGARIYRRLYQK 305

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BLAST of Carg14852 vs. TAIR10
Match: AT5G24840.1 (tRNA (guanine-N-7) methyltransferase)

HSP 1 Score: 65.9 bits (159), Expect = 5.2e-11
Identity = 53/182 (29.12%), Postives = 82/182 (45.05%), Query Frame = 0


Query: 94  RIRQHVNPLSSS-FSVPTPAPNWDEVFKDPTL----------PLMVDIGSGSGRFLLWLA 153
           R R H NPLS S F +P    + D     P                DIG G G  L+ LA
Sbjct: 23  RARAHSNPLSDSHFPIPISPAHVDYSLHFPKFVEADNKFIKKVEFADIGCGFGGLLISLA 82

Query: 154 KKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMFANATI----SFKQIISSY-PGP 213
               D+   +G+E+R K+ +     +  L   +    + N ++    S K I + +  G 
Sbjct: 83  TLFPDTL-MIGMELRDKVTEYVKERILALRRTSSEGQYENISVVRTNSMKYIPNYFEKGQ 142

Query: 214 LMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDS 260
           L  +  L PDPHFK+++H+RRV+   L+D     L  GG ++  +DV E+   M+   + 
Sbjct: 143 LSKMFFLFPDPHFKEKNHRRRVISTHLLDEYAYVLRAGGIIYTITDVEELGEWMKSCLEK 202

[Close]

BLAST of Carg14852 vs. Swiss-Prot
Match: sp|Q2JJQ0|TRMB_SYNJB (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=trmB PE=3 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 5.3e-45
Identity = 102/214 (47.66%), Postives = 133/214 (62.15%), Query Frame = 0


Query: 91  GHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRN 150
           GH R+RQHVNPL + +  P P P+W+ V++    P  +DIG+GSGRFLL +A++  D  N
Sbjct: 35  GH-RVRQHVNPLQAQYQQPAPPPHWERVYRRLGQPFHLDIGTGSGRFLLRIAQEQPD-WN 94

Query: 151 FLGLEIRQKLVKRADVWVKELGFNNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHF 210
           FLG+EIRQ LV+RA+ W  ELG +NVHF+FAN  +S + + +  PG L  V+IL PDP F
Sbjct: 95  FLGVEIRQALVERANAWRDELGLDNVHFLFANINVSLRHLFA--PGDLSRVTILFPDPWF 154

Query: 211 KKRHHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQ 270
           KKRHHKRR++Q  LV  +   L PG  VF+QSD+ EVA +M   F      L+H    D 
Sbjct: 155 KKRHHKRRIVQPQLVADLALLLRPGSPVFLQSDIQEVAEEMAARF------LEHPQFWD- 214

Query: 271 SLLCDKEGWLLKNPMGIRTEREVHAESEGANIYR 305
                 +G L  NP+GI TERE         IYR
Sbjct: 215 ----PHQGPLDSNPLGIPTEREWQCLQLDLPIYR 233

[Close]

BLAST of Carg14852 vs. Swiss-Prot
Match: sp|Q2JRH1|TRMB_SYNJA (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=trmB PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 9.0e-45
Identity = 102/211 (48.34%), Postives = 132/211 (62.56%), Query Frame = 0


Query: 94  RIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLG 153
           R+RQHVNPL + +  PTP P+W+ V++    PL +DIGSGSGRFLL +A++  D  NFLG
Sbjct: 34  RLRQHVNPLQAQYQQPTPPPHWERVYRRLGQPLHLDIGSGSGRFLLRMAQEQPD-WNFLG 93

Query: 154 LEIRQKLVKRADVWVKELGFNNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKR 213
           +EIRQ LV+RA+ W  ELG +NVHF+FAN  +S + + +  PG L  V+I  PDP FKKR
Sbjct: 94  VEIRQPLVERANAWRDELGLDNVHFLFANINVSLRHLFA--PGDLSRVTIQFPDPWFKKR 153

Query: 214 HHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLL 273
           HHKRR++Q  LV  +   L PG  VF+QSDV EVA +M      V   L+H    +    
Sbjct: 154 HHKRRIVQPRLVADLALLLQPGSPVFLQSDVREVAEEM------VDRFLEHPQFWN---- 213

Query: 274 CDKEGWLLKNPMGIRTEREVHAESEGANIYR 305
              +G +  NP GI TERE      G  IYR
Sbjct: 214 -PYQGPIDDNPFGIPTEREWQCLQLGLPIYR 230

[Close]

BLAST of Carg14852 vs. Swiss-Prot
Match: sp|P73161|TRMB_SYNY3 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=trmB PE=3 SV=2)

HSP 1 Score: 166.8 bits (421), Expect = 3.9e-40
Identity = 93/217 (42.86%), Postives = 126/217 (58.06%), Query Frame = 0


Query: 93  VRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFL 152
           VRIRQHVNPLS  +      P+W  V+ D   PL +DIG   GRF L +A++H D  NFL
Sbjct: 4   VRIRQHVNPLSEKYRQVLACPDWATVYDDVQRPLHLDIGCARGRFPLKMAQQHPD-WNFL 63

Query: 153 GLEIRQKLVKRADVWVKELGFNNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKK 212
           G+EIRQ LV  A+     LG  N+H++F N  +  ++  S++P  L  VSI  PDP FK+
Sbjct: 64  GVEIRQPLVLEANETGDRLGLKNLHYLFGNINVEPEKFFSAFPPTLQRVSIQFPDPWFKQ 123

Query: 213 RHHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSL 272
           RH+KRRV Q  LV +I ++L PGG+V +QSDV  VA DMR  F             D  +
Sbjct: 124 RHNKRRVAQPELVTAIANALPPGGEVLLQSDVEPVAQDMRDRFAE----------NDNFV 183

Query: 273 LCDKEGWLLKNPMGIRTEREVHAESEGANIYRRMYQK 310
                 WL +NP+G+ TERE+   +    +YR + Q+
Sbjct: 184 FTHDTPWLAENPLGVPTEREIACFNLQRPVYRCLLQR 209

[Close]

BLAST of Carg14852 vs. Swiss-Prot
Match: sp|P72546|TRMB_SYNE7 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus elongatus (strain PCC 7942) OX=1140 GN=trmB PE=3 SV=2)

HSP 1 Score: 161.4 bits (407), Expect = 1.6e-38
Identity = 91/216 (42.13%), Postives = 128/216 (59.26%), Query Frame = 0


Query: 93  VRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFL 152
           VR+RQHVNPLS  F V T  P+W +V+ D   PL +DIG   GRFLL +A       N+L
Sbjct: 4   VRVRQHVNPLSQKFQVVTTWPDWQQVYADCDRPLHLDIGCARGRFLLAMATAQ-PEWNYL 63

Query: 153 GLEIRQKLVKRADVWVKELGFNNVHFMFANATISFKQIISSYP-GPLMLVSILCPDPHFK 212
           GLEIR+ LV  A+   +E    N+++ F+NA +  + ++ S P G L  VSI  PDP FK
Sbjct: 64  GLEIREPLVDEANAIARERELTNLYYHFSNANLDLEPLLRSLPTGILQRVSIQFPDPWFK 123

Query: 213 KRHHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQS 272
           KRH KRRV+Q  LV ++  +L  G +VF+QSDVLEV  +M   F + P   +    LD  
Sbjct: 124 KRHQKRRVVQPELVQALATALPAGAEVFLQSDVLEVQAEMCEHFAAEPRFQR--TCLD-- 183

Query: 273 LLCDKEGWLLKNPMGIRTEREVHAESEGANIYRRMY 308
                  WL +NP+ + TERE+  +++   +YR ++
Sbjct: 184 -------WLPENPLPVPTEREIAVQNKQLPVYRALF 207

[Close]

BLAST of Carg14852 vs. Swiss-Prot
Match: sp|Q5N2X5|TRMB_SYNP6 (tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=trmB PE=3 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.6e-38
Identity = 91/216 (42.13%), Postives = 128/216 (59.26%), Query Frame = 0


Query: 93  VRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFL 152
           VR+RQHVNPLS  F V T  P+W +V+ D   PL +DIG   GRFLL +A       N+L
Sbjct: 4   VRVRQHVNPLSQKFQVVTTWPDWQQVYADCDRPLHLDIGCARGRFLLAMATAQ-PEWNYL 63

Query: 153 GLEIRQKLVKRADVWVKELGFNNVHFMFANATISFKQIISSYP-GPLMLVSILCPDPHFK 212
           GLEIR+ LV  A+   +E    N+++ F+NA +  + ++ S P G L  VSI  PDP FK
Sbjct: 64  GLEIREPLVDEANAIARERELTNLYYHFSNANLDLEPLLRSLPTGILQRVSIQFPDPWFK 123

Query: 213 KRHHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQS 272
           KRH KRRV+Q  LV ++  +L  G +VF+QSDVLEV  +M   F + P   +    LD  
Sbjct: 124 KRHQKRRVVQPELVQALATALPAGAEVFLQSDVLEVQAEMCEHFAAEPRFQR--TCLD-- 183

Query: 273 LLCDKEGWLLKNPMGIRTEREVHAESEGANIYRRMY 308
                  WL +NP+ + TERE+  +++   +YR ++
Sbjct: 184 -------WLPENPLPVPTEREIAVQNKQLPVYRALF 207

[Close]

BLAST of Carg14852 vs. TrEMBL
Match: tr|A0A1S3AVT2|A0A1S3AVT2_CUCME (tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483376 PE=4 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.7e-144
Identity = 256/309 (82.85%), Postives = 276/309 (89.32%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAXXXXXXXXXXXVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLC  L TCS LRC+AA           VATAN+ LS   KRPFL++F LPSGSSA+
Sbjct: 1   MASSLCLHLQTCSHLRCLAA---AKPAAPSVVATANKILS---KRPFLRNFSLPSGSSAD 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRS ELVALEYADLNLSHK+ ED GHVRIRQHVNPLSSSFSVPTPAPNWDEVF+
Sbjct: 61  LCERQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFR 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DPTLPLMVDIGSGSGRFLLWLAK HL+SRNFLGLEIRQKLVKRA  WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKSHLESRNFLGLEIRQKLVKRAGFWVKELGFSNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPL+LVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLLLVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVA DMRLWFDS  D+L+H+D +D+S+LCDK+GWLL NPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAADMRLWFDSEVDILEHIDKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQK 310
           NIYRRMY K
Sbjct: 301 NIYRRMYSK 303

[Close]

BLAST of Carg14852 vs. TrEMBL
Match: tr|A0A0A0L947|A0A0A0L947_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G126850 PE=4 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.7e-141
Identity = 252/309 (81.55%), Postives = 274/309 (88.67%), Query Frame = 0


Query: 1   MASSLCFQLHTCSQLRCIAAXXXXXXXXXXXVATANRTLSLLVKRPFLQSFCLPSGSSAE 60
           MASSLC  L   S LRCIAA           VATAN+ LS   KRPFL++F LPSGSSA+
Sbjct: 1   MASSLCLHLQASSHLRCIAA---AKPAAPSMVATANKILS---KRPFLRNFSLPSGSSAD 60

Query: 61  LCERQQLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFK 120
           LCERQ LRS ELVALEYADLNLSHK+ ED GHVRIRQHVNPLSSSFSVPTPAPNWDEVF+
Sbjct: 61  LCERQPLRSSELVALEYADLNLSHKMVEDLGHVRIRQHVNPLSSSFSVPTPAPNWDEVFR 120

Query: 121 DPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMF 180
           DPTLPLMVDIGSGSGRFLLWLAK+H +SRNFLGLEIRQKLVKRA +WVKELGF+NVHFMF
Sbjct: 121 DPTLPLMVDIGSGSGRFLLWLAKRHPESRNFLGLEIRQKLVKRAGIWVKELGFSNVHFMF 180

Query: 181 ANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFI 240
           ANATISFKQIISSYPGPL+LVSILCPDPHFKKRHHKRRVLQKPLV+SI+DSLMPGGQVFI
Sbjct: 181 ANATISFKQIISSYPGPLILVSILCPDPHFKKRHHKRRVLQKPLVESIVDSLMPGGQVFI 240

Query: 241 QSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGA 300
           QSDVLEVA DMRLWFDS   +L+H++ +D+S+LCDK+GWLL NPMGIRTERE+HAESEGA
Sbjct: 241 QSDVLEVAADMRLWFDSEAHILEHINKIDKSVLCDKDGWLLNNPMGIRTEREIHAESEGA 300

Query: 301 NIYRRMYQK 310
           NIYRRMY K
Sbjct: 301 NIYRRMYSK 303

[Close]

BLAST of Carg14852 vs. TrEMBL
Match: tr|A0A2I4EE21|A0A2I4EE21_9ROSI (uncharacterized protein LOC108988752 OS=Juglans regia OX=51240 GN=LOC108988752 PE=4 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 2.3e-109
Identity = 195/284 (68.66%), Postives = 232/284 (81.69%), Query Frame = 0


Query: 34  TANRTLSLLVKRPFLQS---FC---LPSGSSAELCERQQLRSPELVALEYADLNLSHKIF 93
           T N  L  LV+R    S   +C   +   ++A    R  LRSP+LVA EYA+LNL H++ 
Sbjct: 22  TTNEALCALVRRQSSLSSRHYCSKRVAGCAAAAAKHRIYLRSPDLVASEYAELNLPHQVS 81

Query: 94  EDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLD 153
           E+ GH+RIRQHVNPLS+ FS+P   P+W+EVF+DPTLPLMVDIGSGSGRFL+WLAK+H +
Sbjct: 82  EELGHIRIRQHVNPLSACFSMPVEVPDWNEVFRDPTLPLMVDIGSGSGRFLIWLAKRHPE 141

Query: 154 SRNFLGLEIRQKLVKRADVWVKELGFNNVHFMFANATISFKQIISSYPGPLMLVSILCPD 213
            RN+LGLEIRQKLV RAD WVKEL   NVHF+FANA +SF Q++S+YPGPL+LVSILCPD
Sbjct: 142 VRNYLGLEIRQKLVNRADFWVKELALYNVHFLFANAMVSFNQLVSTYPGPLVLVSILCPD 201

Query: 214 PHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDV 273
           PHFKKRHHKRRV+QKPLVDSII +LMPGGQ+FIQSDVLEVA+DMR  FD+  DVL H+D 
Sbjct: 202 PHFKKRHHKRRVVQKPLVDSIIHNLMPGGQIFIQSDVLEVALDMRYQFDAEGDVLHHMDA 261

Query: 274 LDQSLLCDKEGWLLKNPMGIRTEREVHAESEGANIYRRMYQKYV 312
           LDQS+LCD+EGWLL +PMGIRTERE+HAE EGA IYRRMYQK +
Sbjct: 262 LDQSVLCDQEGWLLSSPMGIRTEREIHAEFEGARIYRRMYQKRI 305

[Close]

BLAST of Carg14852 vs. TrEMBL
Match: tr|D7UB80|D7UB80_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_15s0024g00790 PE=4 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 2.5e-108
Identity = 188/262 (71.76%), Postives = 226/262 (86.26%), Query Frame = 0


Query: 51  FCLPSGSSAELCERQQLRSPELVALEYADLNLSH-KIFEDSGHVRIRQHVNPLSSSFSVP 110
           FC  +  +A     +QL SPELVALEYADLNL++  + ++ GHVRIRQHVNP + SFS+P
Sbjct: 50  FC--NSPTAVASTAKQLTSPELVALEYADLNLNYDNVSQEVGHVRIRQHVNPFAPSFSIP 109

Query: 111 TPAPNWDEVFKDPTLPLMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVK 170
            P PNW +VFKD TLPLMVDIGSGSGRFL+WLAK++ DS+N+LGLEIRQKL KRA+ WVK
Sbjct: 110 APVPNWCQVFKDSTLPLMVDIGSGSGRFLMWLAKRNPDSQNYLGLEIRQKLAKRAEFWVK 169

Query: 171 ELGFNNVHFMFANATISFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSII 230
           EL  +N+HFMFANATISFK+++S+YPGPLMLVS+LCPDPHFKKRHHKRRV+QKPLVDSI+
Sbjct: 170 ELSLHNIHFMFANATISFKELVSAYPGPLMLVSLLCPDPHFKKRHHKRRVVQKPLVDSIV 229

Query: 231 DSLMPGGQVFIQSDVLEVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRT 290
            +LMPGGQVFIQSDVLEVAVDMR  FD+  D+L+H+D +D S+ CD+EGWL+KNPMGIRT
Sbjct: 230 KNLMPGGQVFIQSDVLEVAVDMRDRFDAESDLLQHIDTIDPSMQCDEEGWLVKNPMGIRT 289

Query: 291 EREVHAESEGANIYRRMYQKYV 312
           ERE+HAE EGA IYRR+YQK +
Sbjct: 290 EREIHAEFEGAKIYRRLYQKLI 309

[Close]

BLAST of Carg14852 vs. TrEMBL
Match: tr|A0A2P6SDJ0|A0A2P6SDJ0_ROSCH (Putative tRNA (Guanine(46)-N(7))-methyltransferase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0340701 PE=4 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 4.3e-108
Identity = 184/246 (74.80%), Postives = 221/246 (89.84%), Query Frame = 0


Query: 66  QLRSPELVALEYADLNLSHKIFEDSGHVRIRQHVNPLSSSFSVPTPAPNWDEVFKDPTLP 125
           +LRSP+LVALEYADLNL+H + ++ GHVRIRQHVNPLSSS SVP   P+W++VF+DPTLP
Sbjct: 64  ELRSPDLVALEYADLNLTHNVSQELGHVRIRQHVNPLSSSLSVPVEVPDWNQVFRDPTLP 123

Query: 126 LMVDIGSGSGRFLLWLAKKHLDSRNFLGLEIRQKLVKRADVWVKELGFNNVHFMFANATI 185
           LMVDIGSGSGRFL+WLAK++L  RN+LGLEIRQKLVKRADVWVKELG +N+HFM+ANAT 
Sbjct: 124 LMVDIGSGSGRFLMWLAKRNLVVRNYLGLEIRQKLVKRADVWVKELGLSNIHFMYANATT 183

Query: 186 SFKQIISSYPGPLMLVSILCPDPHFKKRHHKRRVLQKPLVDSIIDSLMPGGQVFIQSDVL 245
           SF Q++S+YPGPLMLVSILCPDP+FK++HHKRRV+QKPLVDSI+++L P GQVFIQSDVL
Sbjct: 184 SFNQLVSTYPGPLMLVSILCPDPYFKRKHHKRRVVQKPLVDSIVNNLAPRGQVFIQSDVL 243

Query: 246 EVAVDMRLWFDSVPDVLKHVDVLDQSLLCDKEGWLLKNPMGIRTEREVHAESEGANIYRR 305
           +VA++MR  FDS+P VLKH+D ++ S+LCD EGWLL NPMG RTERE+HAE EGA IYRR
Sbjct: 244 DVALEMRNQFDSLPHVLKHIDEIESSVLCDSEGWLLSNPMGKRTEREIHAEFEGAKIYRR 303

Query: 306 MYQKYV 312
           +YQK V
Sbjct: 304 VYQKQV 309

The following BLAST results are available for this feature:

BLAST of Carg14852 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023527130.1	5.3e-171	95.53	uncharacterized protein LOC111790461 [Cucurbita pepo subsp. pepo]	[more]
XP_022924717.1	5.9e-170	98.72	uncharacterized protein LOC111432133 [Cucurbita moschata]	[more]
XP_022980361.1	6.5e-169	98.08	uncharacterized protein LOC111479752 [Cucurbita maxima]	[more]
XP_022146903.1	8.6e-145	82.69	uncharacterized protein LOC111015991 [Momordica charantia]	[more]
XP_008438199.1	2.5e-144	82.85	PREDICTED: tRNA (guanine-N(7)-)-methyltransferase [Cucumis melo]	[more]

BLAST of Carg14852 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G17660.1	2.9e-102	69.14	tRNA (guanine-N-7) methyltransferase	[more]
AT5G24840.1	5.2e-11	29.12	tRNA (guanine-N-7) methyltransferase	[more]

BLAST of Carg14852 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q2JJQ0\|TRMB_SYNJB	5.3e-45	47.66	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-2-3B'a(2-...	[more]
sp\|Q2JRH1\|TRMB_SYNJA	9.0e-45	48.34	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain JA-3-3Ab) OX...	[more]
sp\|P73161\|TRMB_SYNY3	3.9e-40	42.86	tRNA (guanine-N(7)-)-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / K...	[more]
sp\|P72546\|TRMB_SYNE7	1.6e-38	42.13	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus elongatus (strain PCC 79...	[more]
sp\|Q5N2X5\|TRMB_SYNP6	1.6e-38	42.13	tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. (strain ATCC 27144 /...	[more]

BLAST of Carg14852 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3AVT2\|A0A1S3AVT2_CUCME	1.7e-144	82.85	tRNA (Guanine-N(7)-)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483376 P...	[more]
tr\|A0A0A0L947\|A0A0A0L947_CUCSA	1.7e-141	81.55	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G126850 PE=4 SV=1	[more]
tr\|A0A2I4EE21\|A0A2I4EE21_9ROSI	2.3e-109	68.66	uncharacterized protein LOC108988752 OS=Juglans regia OX=51240 GN=LOC108988752 P...	[more]
tr\|D7UB80\|D7UB80_VITVI	2.5e-108	71.76	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_15s0024g00790 PE=4 SV=...	[more]
tr\|A0A2P6SDJ0\|A0A2P6SDJ0_ROSCH	4.3e-108	74.80	Putative tRNA (Guanine(46)-N(7))-methyltransferase OS=Rosa chinensis OX=74649 GN...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008176	tRNA (guanine-N7-)-methyltransferase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006400	tRNA modification

Vocabulary: **INTERPRO**
Term	Definition
IPR029063	SAM-dependent_MTases
IPR003358	tRNA_(Gua-N-7)_MeTrfase_Trmb

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008176	tRNA (guanine-N7-)-methyltransferase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg14852-RA	Carg14852-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.40.50.150		coord: 95..311 e-value: 2.2E-54 score: 185.9
None	No IPR available	PFAM	PF02390	Methyltransf_4	coord: 125..304 e-value: 2.2E-32 score: 111.8
None	No IPR available	PANTHER	PTHR23417:SF21	SUBFAMILY NOT NAMED	coord: 11..309
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 128..241 e-value: 6.21545E-4 score: 36.6391
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	PANTHER	PTHR23417	3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE	coord: 11..309
IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	PROSITE	PS51625	SAM_MT_TRMB	coord: 83..309 score: 52.84
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	SSF53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 125..308

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg14852	Cla021329	Watermelon (97103) v1	carwmB0617
Carg14852	Csa3G126850	Cucumber (Chinese Long) v2	carcuB0644
Carg14852	MELO3C006212	Melon (DHL92) v3.5.1	carmeB0622
Carg14852	ClCG05G002480	Watermelon (Charleston Gray)	carwcgB0602
Carg14852	CSPI03G09770	Wild cucumber (PI 183967)	carcpiB0651
Carg14852	Cucsa.257430	Cucumber (Gy14) v1	carcgyB0693
Carg14852	CmaCh14G019640	Cucurbita maxima (Rimu)	carcmaB0874
Carg14852	CmoCh14G020240	Cucurbita moschata (Rifu)	carcmoB0850
Carg14852	Lsi05G019360	Bottle gourd (USVL1VR-Ls)	carlsiB565
Carg14852	Cp4.1LG03g17280	Cucurbita pepo (Zucchini)	carcpeB0884
Carg14852	CsGy3G009860	Cucumber (Gy14) v2	carcgybB0617
Carg14852	MELO3C006212.2	Melon (DHL92) v3.6.1	carmedB0629
Carg14852	CsaV3_3G009910	Cucumber (Chinese Long) v3	carcucB0652
Carg14852	Cla97C05G082980	Watermelon (97103) v2	carwmbB0647
Carg14852	Bhi01G000051	Wax gourd	carwgoB0737

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg14852	Silver-seed gourd	carcarB261
Carg14852	Silver-seed gourd	carcarB549
Carg14852	Silver-seed gourd	carcarB550
Carg14852	Cucumber (Gy14) v2	carcgybB0619
Carg14852	Cucumber (Gy14) v1	carcgyB0695
Carg14852	Cucurbita maxima (Rimu)	carcmaB0876
Carg14852	Cucurbita maxima (Rimu)	carcmaB0877
Carg14852	Cucurbita maxima (Rimu)	carcmaB0880
Carg14852	Cucurbita moschata (Rifu)	carcmoB0851
Carg14852	Cucurbita moschata (Rifu)	carcmoB0852
Carg14852	Cucurbita moschata (Rifu)	carcmoB0854
Carg14852	Cucurbita pepo (Zucchini)	carcpeB0881
Carg14852	Cucurbita pepo (Zucchini)	carcpeB0882
Carg14852	Cucurbita pepo (Zucchini)	carcpeB0885
Carg14852	Wild cucumber (PI 183967)	carcpiB0653
Carg14852	Cucumber (Chinese Long) v2	carcuB0645
Carg14852	Cucumber (Chinese Long) v3	carcucB0654
Carg14852	Bottle gourd (USVL1VR-Ls)	carlsiB564
Carg14852	Melon (DHL92) v3.5.1	carmeB0621
Carg14852	Melon (DHL92) v3.6.1	carmedB0628
Carg14852	Watermelon (Charleston Gray)	carwcgB0603
Carg14852	Watermelon (97103) v1	carwmB0619
Carg14852	Watermelon (97103) v2	carwmbB0648
Carg14852	Wax gourd	carwgoB0736