Carg13751 (gene) Silver-seed gourd

Overview
NameCarg13751
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionEndonuclease
LocationCucurbita_argyrosperma_scaffold_058 : 359698 .. 361589 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AACTATCCATTTTCTAGATTTACGCTCGTAACCTCTGATTGGCTGAGAGGCAAGCGTCGAAGAACGAGTATAATTTTGCAGGCGAGCTTCAGAGCCGACGGTCAAGTAGGTGAAGTCGGAGCTGCAATCACTGGGAAGAGTAGGGTTCCGATTTCACTGTGTTGATTGCGGAAATGGGCCAATCTGAGCTTTGCTTGATCGCGAAAGCGTTCGCATTCCTGCTACTTGTACCTGGAATCCTTGGCTGGGGAAAAGAAGGTCACTACCTAATTTGCAAGATAGCGGAGGTAAGCATCTGTTGCGTTTGATTTTGTCAAGCTTATCGATGTTACAGAAGGTTTATAGGAAAGGATTATAGCTGATGGGATTTTGGTTTCGGATTCTTGAAACTGTAGCTATCGCAGTTATAATAGCGTAGGCCAGGATTCGATTACATTAAACATGGTGAAGGAGAGAGGATTCTTGTGAAATTTGGTTAGCTATTAGAGAACTTTTTGACCATTTGGATTTTTTTTTTGCATAAAAACTGAAGTAAATTCTTTTAGCCACTATATCAGGTCCTTATTAAGCTACAAACTTTTAGTGGGGAGTTGTTTTGGAGCTTATTATAACTTATATCACCCTATGATTAAGATTGCAGCCCTTGGATCCTGTAAAAATTTTGTTGTAGTCAAAATCTGATGTCACTTCCTTTGGTTTTAGGGATATTTCACTGAAGATACTCTATCCATGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGAGATCTTGCGGCTGTATGCTCCTGGCCTGATGAAATTCGATGGAAGGCTCATTATCGCTGGAGTAGCGCCCTACACTACGTCGACACGCCTGATTTCTTTTGTAATTATAATTACTCGAGTAAGTTTAGCTTTATGGTATTTTCAGTTTCTCTTGTGGTCTATTATGCCATATAACTGTTTGATATCATGCTTGTTGTTCATGACCTTTAAGTCTCAAATTCGTTGTTGAACCACCCCATCAATACTGAAGCTGAGACATTTTATAGCTCATGATCACAAAACCGATGCATCCTCTAAAGTTGTGTTCTATGGCTAAGGTCTTCACTGTCTAGCTGATTGCTATAGTATTATTGTGAGATCCCATGTCAGTTGGAGAAGGGAACGAAGCATTCCTTATAAGGGTGTGGAAACCTCTCCTTAATAGACGCGTTTTAAAACCGTGAGGCTGACGGCGATACGTAACGGGTCCAAGCGAACAATATCTGCTAGCGGTGGGCTTTAACTGTCACAACTATAGGATCGATGTCTTCTTGAATTATAATTTCCGTATGTATCAGAATCTAAAATTTTGACTTAGAGCAAATTTGTTTTTCAATTCAGGAGCAGTAGCCTGCTCTTGCCTTTCTAGCTTTGAAACATCTAATGTTGACAACTATGAAGTTAACGAAAACCAGTCATTCTGAATATGTCATATACAATGGTTATTAATTCAATTGAGTTTTGATTCCGTTCATTCAACGAGGCATTTTTAGTTGGAAGATTTAGCTCTAATACTAATTTTCAATACTTGAAACTTGAAAGTGCTATTTTGACTATGTCTGCTTGTGTAACAGTATTCTCTGCTTAATATAGGAGACTGTCATGATACTCATGGACATAAGGGTAGATGTGTGACTGCAGCAATTTACAACTATACTGGGCAACTCGAATCAGCCTACAAAGAGATGACTTCAGGAATTAGATGTAAGCATCGTAGAAGACTAACGTTGTATGTATACACTTTTTGAACGAATGTCACTTACATTCACGTGATATTTTCAGATAACTTAACTGAGGCTCTTATGTTCTTGTCCCATTTTATCGGAGATGTCCATCAGGTTTGCTCTTTCTACAGATCTGAACTTTAA

mRNA sequence

AACTATCCATTTTCTAGATTTACGCTCGTAACCTCTGATTGGCTGAGAGGCAAGCGTCGAAGAACGAGTATAATTTTGCAGGCGAGCTTCAGAGCCGACGGTCAAGTAGGTGAAGTCGGAGCTGCAATCACTGGGAAGAGTAGGGTTCCGATTTCACTGTGTTGATTGCGGAAATGGGCCAATCTGAGCTTTGCTTGATCGCGAAAGCGTTCGCATTCCTGCTACTTGTACCTGGAATCCTTGGCTGGGGAAAAGAAGGTCACTACCTAATTTGCAAGATAGCGGAGGGATATTTCACTGAAGATACTCTATCCATGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGAGATCTTGCGGCTGTATGCTCCTGGCCTGATGAAATTCGATGGAAGGCTCATTATCGCTGGAGTAGCGCCCTACACTACGTCGACACGCCTGATTTCTTTTGTAATTATAATTACTCGAGAGACTGTCATGATACTCATGGACATAAGGGTAGATGTGTGACTGCAGCAATTTACAACTATACTGGGCAACTCGAATCAGCCTACAAAGAGATGACTTCAGGAATTAGATATAACTTAACTGAGGCTCTTATGTTCTTGTCCCATTTTATCGGAGATGTCCATCAGGTTTGCTCTTTCTACAGATCTGAACTTTAA

Coding sequence (CDS)

ATGGGCCAATCTGAGCTTTGCTTGATCGCGAAAGCGTTCGCATTCCTGCTACTTGTACCTGGAATCCTTGGCTGGGGAAAAGAAGGTCACTACCTAATTTGCAAGATAGCGGAGGGATATTTCACTGAAGATACTCTATCCATGGTCAAAGAATTGCTTCCAGCTTCTGCTGAGGGAGATCTTGCGGCTGTATGCTCCTGGCCTGATGAAATTCGATGGAAGGCTCATTATCGCTGGAGTAGCGCCCTACACTACGTCGACACGCCTGATTTCTTTTGTAATTATAATTACTCGAGAGACTGTCATGATACTCATGGACATAAGGGTAGATGTGTGACTGCAGCAATTTACAACTATACTGGGCAACTCGAATCAGCCTACAAAGAGATGACTTCAGGAATTAGATATAACTTAACTGAGGCTCTTATGTTCTTGTCCCATTTTATCGGAGATGTCCATCAGGTTTGCTCTTTCTACAGATCTGAACTTTAA

Protein sequence

MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGDLAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYTGQLESAYKEMTSGIRYNLTEALMFLSHFIGDVHQVCSFYRSEL
Homology
[Close]
BLAST of Carg13751 vs. NCBI nr
Match: XP_023543506.1 (endonuclease 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 326.2 bits (835), Expect = 6.1e-86
Identity = 152/154 (98.70%), Postives = 152/154 (98.70%), Query Frame = 0 

Query: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60
           MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD
Sbjct: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120

Query: 121 GQLESAYKEMTSGIRYNLTEALMFLSHFIGDVHQ 155
            QLESAYKEMTS IRYNLTEALMFLSHFIGDVHQ
Sbjct: 121 RQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQ 154

[Close]
BLAST of Carg13751 vs. NCBI nr
Match: XP_022950165.1 (endonuclease 4-like [Cucurbita moschata])

HSP 1 Score: 323.9 bits (829), Expect = 3.0e-85
Identity = 151/154 (98.05%), Postives = 151/154 (98.05%), Query Frame = 0 

Query: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60
           MGQ ELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD
Sbjct: 1   MGQFELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120

Query: 121 GQLESAYKEMTSGIRYNLTEALMFLSHFIGDVHQ 155
            QLESAYKEMTS IRYNLTEALMFLSHFIGDVHQ
Sbjct: 121 RQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQ 154

[Close]
BLAST of Carg13751 vs. NCBI nr
Match: XP_022978904.1 (endonuclease 4-like [Cucurbita maxima])

HSP 1 Score: 323.2 bits (827), Expect = 5.2e-85
Identity = 151/154 (98.05%), Postives = 151/154 (98.05%), Query Frame = 0 

Query: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60
           MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD
Sbjct: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTH HKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHEHKGRCVTAAIYNYT 120

Query: 121 GQLESAYKEMTSGIRYNLTEALMFLSHFIGDVHQ 155
            QLESAYKEMTS IRYNLTEALMFLSHFIGDVHQ
Sbjct: 121 RQLESAYKEMTSEIRYNLTEALMFLSHFIGDVHQ 154

[Close]
BLAST of Carg13751 vs. NCBI nr
Match: XP_022142132.1 (endonuclease 4-like [Momordica charantia])

HSP 1 Score: 283.9 bits (725), Expect = 3.5e-73
Identity = 134/154 (87.01%), Postives = 137/154 (88.96%), Query Frame = 0 

Query: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60
           MG+ E  L  +A  FLLLVPGILGWGKEGHY ICKIAEGYFTED LSMVKELLPASAEGD
Sbjct: 1   MGRFE--LFWRASVFLLLVPGILGWGKEGHYAICKIAEGYFTEDALSMVKELLPASAEGD 60

Query: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEIR KAHY WSS LHYVDTPDFFCNYNYSRDCHDTHGH GRCV AAIYNYT
Sbjct: 61  LAAVCSWPDEIRRKAHYHWSSVLHYVDTPDFFCNYNYSRDCHDTHGHNGRCVIAAIYNYT 120

Query: 121 GQLESAYKEMTSGIRYNLTEALMFLSHFIGDVHQ 155
            QLES YKEMTS +RYNLTEALMFLSHFIGDVHQ
Sbjct: 121 MQLESTYKEMTSDVRYNLTEALMFLSHFIGDVHQ 152

[Close]
BLAST of Carg13751 vs. NCBI nr
Match: XP_016902623.1 (PREDICTED: endonuclease 4-like isoform X3 [Cucumis melo])

HSP 1 Score: 276.2 bits (705), Expect = 7.3e-71
Identity = 127/155 (81.94%), Postives = 136/155 (87.74%), Query Frame = 0 

Query: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60
           MGQSELC  A  F FLLL+PGILGWG+EGHY +CKIAEGY TED LSMV+ELLPASAEGD
Sbjct: 21  MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAEGYLTEDALSMVRELLPASAEGD 80

Query: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSW DE+R    Y WSS LH+VDTPDFFCNYN SRDCHD+H HKGRCVTAAIYNYT
Sbjct: 81  LAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSRDCHDSHRHKGRCVTAAIYNYT 140

Query: 121 GQLESAYKEMTSGIRYNLTEALMFLSHFIGDVHQV 156
            QLESAYKE+TS +RYNLTEALMFLSHFIGDVHQV
Sbjct: 141 MQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQV 175

[Close]
BLAST of Carg13751 vs. TAIR10
Match: AT4G21585.1 (endonuclease 4)

HSP 1 Score: 206.1 bits (523), Expect = 1.7e-53
Identity = 92/145 (63.45%), Postives = 112/145 (77.24%), Query Frame = 0 

Query: 10  AKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGDLAAVCSWPD 69
           A+      L+ G L WGKEGHY +CKIAE YF E+T++ VK+LLP SA+GDLA+VCSWPD
Sbjct: 10  ARVLVLTQLINGALCWGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWPD 69

Query: 70  EIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYTGQLESAYKE 129
           EI+    +RW+S LHYVDTPD+ CNY Y RDCHDTH ++ RCVT AI+NYT QL SA + 
Sbjct: 70  EIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASEN 129

Query: 130 MTSGIRYNLTEALMFLSHFIGDVHQ 155
             + + YNLTEALMFLSHFIGD+HQ
Sbjct: 130 SDTIVHYNLTEALMFLSHFIGDIHQ 154

[Close]
BLAST of Carg13751 vs. TAIR10
Match: AT4G21600.1 (endonuclease 5)

HSP 1 Score: 188.7 bits (478), Expect = 2.8e-48
Identity = 83/138 (60.14%), Postives = 107/138 (77.54%), Query Frame = 0 

Query: 18  LVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAE-GDLAAVCSWPDEIRWKAH 77
           LV G L WGK+GHY +CK+AEG+F +DT++ VK+LLP S + G LA  CSWPDEI+  + 
Sbjct: 14  LVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFCSWPDEIKKLSQ 73

Query: 78  YRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYTGQLESAYKEMTSGIRY 137
           ++W+S LHYV+TP++ CNY Y RDCHDTH HK  CVT AI+NYT QL SA +   + + Y
Sbjct: 74  WQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMSASENSQNIVHY 133

Query: 138 NLTEALMFLSHFIGDVHQ 155
           NLTEAL+FLSH++GDVHQ
Sbjct: 134 NLTEALLFLSHYMGDVHQ 151

[Close]
BLAST of Carg13751 vs. TAIR10
Match: AT4G21590.1 (endonuclease 3)

HSP 1 Score: 182.6 bits (462), Expect = 2.0e-46
Identity = 88/154 (57.14%), Postives = 104/154 (67.53%), Query Frame = 0 

Query: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60
           MG S    I        LV G L WG  GHY +CKIA+ YF EDT+  VK+LLP SA G+
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGE 60

Query: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEI+    +RW+SALH+ DTPD+ CNY YSRDC      K  CVT AI+NYT
Sbjct: 61  LAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYT 120

Query: 121 GQLESAYKEMTSGIRYNLTEALMFLSHFIGDVHQ 155
            QL S  +   S + YNLTEALMFLSH++GD+HQ
Sbjct: 121 NQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQ 149

[Close]
BLAST of Carg13751 vs. TAIR10
Match: AT1G68290.1 (endonuclease 2)

HSP 1 Score: 167.9 bits (424), Expect = 5.1e-42
Identity = 79/139 (56.83%), Postives = 98/139 (70.50%), Query Frame = 0 

Query: 16  LLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGDLAAVCSWPDEIRWKA 75
           L   P I GWGKEGH +ICKIA+    E     VKELLP SAEGDL+++C W D ++++ 
Sbjct: 19  LYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCLWADRVKFR- 78

Query: 76  HYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYTGQLESAYKEMTSGIR 135
            Y WSS LHY++TPD  C+Y Y+RDC D  G KGRCV  AIYNYT QL S     +S  +
Sbjct: 79  -YHWSSPLHYINTPD-ACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKTAASSQSQ 138

Query: 136 YNLTEALMFLSHFIGDVHQ 155
           YNLTEAL+F+SHF+GD+HQ
Sbjct: 139 YNLTEALLFVSHFMGDIHQ 154

[Close]
BLAST of Carg13751 vs. TAIR10
Match: AT1G11190.1 (bifunctional nuclease i)

HSP 1 Score: 164.5 bits (415), Expect = 5.6e-41
Identity = 76/147 (51.70%), Postives = 96/147 (65.31%), Query Frame = 0 

Query: 8   LIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGDLAAVCSW 67
           ++      L  V  +  W KEGH L C+IA+         +V+ LLP   +GDL+A+C W
Sbjct: 12  ILVLGILILCSVSSVRSWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALCVW 71

Query: 68  PDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYTGQLESAY 127
           PD+IR    YRW+S LHY+DTPD  C+Y YSRDCHD HG K  CV  AI N+T QL+  Y
Sbjct: 72  PDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQH-Y 131

Query: 128 KEMTSGIRYNLTEALMFLSHFIGDVHQ 155
            E TS  RYN+TEAL+FLSHF+GD+HQ
Sbjct: 132 GEGTSDRRYNMTEALLFLSHFMGDIHQ 157

[Close]
BLAST of Carg13751 vs. Swiss-Prot
Match: sp|F4JJL0|ENDO4_ARATH (Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=3 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 3.0e-52
Identity = 92/145 (63.45%), Postives = 112/145 (77.24%), Query Frame = 0 

Query: 10  AKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGDLAAVCSWPD 69
           A+      L+ G L WGKEGHY +CKIAE YF E+T++ VK+LLP SA+GDLA+VCSWPD
Sbjct: 10  ARVLVLTQLINGALCWGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWPD 69

Query: 70  EIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYTGQLESAYKE 129
           EI+    +RW+S LHYVDTPD+ CNY Y RDCHDTH ++ RCVT AI+NYT QL SA + 
Sbjct: 70  EIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASEN 129

Query: 130 MTSGIRYNLTEALMFLSHFIGDVHQ 155
             + + YNLTEALMFLSHFIGD+HQ
Sbjct: 130 SDTIVHYNLTEALMFLSHFIGDIHQ 154

[Close]
BLAST of Carg13751 vs. Swiss-Prot
Match: sp|F4JJL3|ENDO5_ARATH (Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 5.0e-47
Identity = 83/138 (60.14%), Postives = 107/138 (77.54%), Query Frame = 0 

Query: 18  LVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAE-GDLAAVCSWPDEIRWKAH 77
           LV G L WGK+GHY +CK+AEG+F +DT++ VK+LLP S + G LA  CSWPDEI+  + 
Sbjct: 14  LVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFCSWPDEIKKLSQ 73

Query: 78  YRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYTGQLESAYKEMTSGIRY 137
           ++W+S LHYV+TP++ CNY Y RDCHDTH HK  CVT AI+NYT QL SA +   + + Y
Sbjct: 74  WQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMSASENSQNIVHY 133

Query: 138 NLTEALMFLSHFIGDVHQ 155
           NLTEAL+FLSH++GDVHQ
Sbjct: 134 NLTEALLFLSHYMGDVHQ 151

[Close]
BLAST of Carg13751 vs. Swiss-Prot
Match: sp|Q8LDW6|ENDO3_ARATH (Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 3.6e-45
Identity = 88/154 (57.14%), Postives = 104/154 (67.53%), Query Frame = 0 

Query: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60
           MG S    I        LV G L WG  GHY +CKIA+ YF EDT+  VK+LLP SA G+
Sbjct: 1   MGWSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGE 60

Query: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEI+    +RW+SALH+ DTPD+ CNY YSRDC      K  CVT AI+NYT
Sbjct: 61  LAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYT 120

Query: 121 GQLESAYKEMTSGIRYNLTEALMFLSHFIGDVHQ 155
            QL S  +   S + YNLTEALMFLSH++GD+HQ
Sbjct: 121 NQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQ 149

[Close]
BLAST of Carg13751 vs. Swiss-Prot
Match: sp|Q9C9G4|ENDO2_ARATH (Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 9.1e-41
Identity = 79/139 (56.83%), Postives = 98/139 (70.50%), Query Frame = 0 

Query: 16  LLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGDLAAVCSWPDEIRWKA 75
           L   P I GWGKEGH +ICKIA+    E     VKELLP SAEGDL+++C W D ++++ 
Sbjct: 19  LYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCLWADRVKFR- 78

Query: 76  HYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYTGQLESAYKEMTSGIR 135
            Y WSS LHY++TPD  C+Y Y+RDC D  G KGRCV  AIYNYT QL S     +S  +
Sbjct: 79  -YHWSSPLHYINTPD-ACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYKTAASSQSQ 138

Query: 136 YNLTEALMFLSHFIGDVHQ 155
           YNLTEAL+F+SHF+GD+HQ
Sbjct: 139 YNLTEALLFVSHFMGDIHQ 154

[Close]
BLAST of Carg13751 vs. Swiss-Prot
Match: sp|Q9SXA6|ENDO1_ARATH (Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.0e-39
Identity = 76/147 (51.70%), Postives = 96/147 (65.31%), Query Frame = 0 

Query: 8   LIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGDLAAVCSW 67
           ++      L  V  +  W KEGH L C+IA+         +V+ LLP   +GDL+A+C W
Sbjct: 12  ILVLGILILCSVSSVRSWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALCVW 71

Query: 68  PDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYTGQLESAY 127
           PD+IR    YRW+S LHY+DTPD  C+Y YSRDCHD HG K  CV  AI N+T QL+  Y
Sbjct: 72  PDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQH-Y 131

Query: 128 KEMTSGIRYNLTEALMFLSHFIGDVHQ 155
            E TS  RYN+TEAL+FLSHF+GD+HQ
Sbjct: 132 GEGTSDRRYNMTEALLFLSHFMGDIHQ 157

[Close]
BLAST of Carg13751 vs. TrEMBL
Match: tr|A0A1S4E326|A0A1S4E326_CUCME (endonuclease 4-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103500060 PE=4 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 4.8e-71
Identity = 127/155 (81.94%), Postives = 136/155 (87.74%), Query Frame = 0 

Query: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60
           MGQSELC  A  F FLLL+PGILGWG+EGHY +CKIAEGY TED LSMV+ELLPASAEGD
Sbjct: 21  MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAEGYLTEDALSMVRELLPASAEGD 80

Query: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSW DE+R    Y WSS LH+VDTPDFFCNYN SRDCHD+H HKGRCVTAAIYNYT
Sbjct: 81  LAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSRDCHDSHRHKGRCVTAAIYNYT 140

Query: 121 GQLESAYKEMTSGIRYNLTEALMFLSHFIGDVHQV 156
            QLESAYKE+TS +RYNLTEALMFLSHFIGDVHQV
Sbjct: 141 MQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQV 175

[Close]
BLAST of Carg13751 vs. TrEMBL
Match: tr|A0A1S3CF84|A0A1S3CF84_CUCME (endonuclease 4-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103500060 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 1.4e-70
Identity = 126/154 (81.82%), Postives = 135/154 (87.66%), Query Frame = 0 

Query: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60
           MGQSELC  A  F FLLL+PGILGWG+EGHY +CKIAEGY TED LSMV+ELLPASAEGD
Sbjct: 1   MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAEGYLTEDALSMVRELLPASAEGD 60

Query: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSW DE+R    Y WSS LH+VDTPDFFCNYN SRDCHD+H HKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSRDCHDSHRHKGRCVTAAIYNYT 120

Query: 121 GQLESAYKEMTSGIRYNLTEALMFLSHFIGDVHQ 155
            QLESAYKE+TS +RYNLTEALMFLSHFIGDVHQ
Sbjct: 121 MQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQ 154

[Close]
BLAST of Carg13751 vs. TrEMBL
Match: tr|A0A1S3CEU3|A0A1S3CEU3_CUCME (endonuclease 4-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500060 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 1.4e-70
Identity = 126/154 (81.82%), Postives = 135/154 (87.66%), Query Frame = 0 

Query: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60
           MGQSELC  A  F FLLL+PGILGWG+EGHY +CKIAEGY TED LSMV+ELLPASAEGD
Sbjct: 21  MGQSELCWTANVFVFLLLLPGILGWGREGHYAVCKIAEGYLTEDALSMVRELLPASAEGD 80

Query: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSW DE+R    Y WSS LH+VDTPDFFCNYN SRDCHD+H HKGRCVTAAIYNYT
Sbjct: 81  LAAVCSWADELRENHDYHWSSVLHFVDTPDFFCNYNCSRDCHDSHRHKGRCVTAAIYNYT 140

Query: 121 GQLESAYKEMTSGIRYNLTEALMFLSHFIGDVHQ 155
            QLESAYKE+TS +RYNLTEALMFLSHFIGDVHQ
Sbjct: 141 MQLESAYKEITSEVRYNLTEALMFLSHFIGDVHQ 174

[Close]
BLAST of Carg13751 vs. TrEMBL
Match: tr|A0A0A0LY83|A0A0A0LY83_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G163110 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 5.0e-68
Identity = 124/154 (80.52%), Postives = 132/154 (85.71%), Query Frame = 0 

Query: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60
           MGQSELC  A AF FLL +PGILGWG+EGHY+ICKIAE Y TED LSMVKELLP+ AEGD
Sbjct: 1   MGQSELCWTANAFLFLLFLPGILGWGREGHYMICKIAEKYLTEDALSMVKELLPSYAEGD 60

Query: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSW DE+R    Y WS ALHYVDTPDFFCNY  SRDCHD + HKGRCVTAAIYNYT
Sbjct: 61  LAAVCSWADELRAHPDYHWSGALHYVDTPDFFCNYKCSRDCHDNYRHKGRCVTAAIYNYT 120

Query: 121 GQLESAYKEMTSGIRYNLTEALMFLSHFIGDVHQ 155
            QLESAYKE+TS I+YNLTEALMFLSHFIGDVHQ
Sbjct: 121 MQLESAYKEITSEIKYNLTEALMFLSHFIGDVHQ 154

[Close]
BLAST of Carg13751 vs. TrEMBL
Match: tr|A0A2I4DJK4|A0A2I4DJK4_9ROSI (endonuclease 4-like OS=Juglans regia OX=51240 GN=LOC108980784 PE=4 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.4e-62
Identity = 116/154 (75.32%), Postives = 124/154 (80.52%), Query Frame = 0 

Query: 1   MGQSELCLIAKAFAFLLLVPGILGWGKEGHYLICKIAEGYFTEDTLSMVKELLPASAEGD 60
           MG+SEL  I  A  FLL VPGILGWGKEGHY ICKIAEGY TED L+ VK LLP SA GD
Sbjct: 1   MGRSELLWIGTALGFLLFVPGILGWGKEGHYAICKIAEGYLTEDALAAVKTLLPESAGGD 60

Query: 61  LAAVCSWPDEIRWKAHYRWSSALHYVDTPDFFCNYNYSRDCHDTHGHKGRCVTAAIYNYT 120
           LAAVCSWPDEIR   H+RWSSALHYVDTPDF CNYNY RDCHD+ GHK  CVT AI NYT
Sbjct: 61  LAAVCSWPDEIRHNFHWRWSSALHYVDTPDFSCNYNYCRDCHDSAGHKDMCVTGAINNYT 120

Query: 121 GQLESAYKEMTSGIRYNLTEALMFLSHFIGDVHQ 155
            QL S+Y+E    ++YNLTEALMFLSHFIGDVHQ
Sbjct: 121 MQLSSSYQEANLKLQYNLTEALMFLSHFIGDVHQ 154

The following BLAST results are available for this feature:
BLAST of Carg13751 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023543506.16.1e-8698.70endonuclease 4-like [Cucurbita pepo subsp. pepo][more]
XP_022950165.13.0e-8598.05endonuclease 4-like [Cucurbita moschata][more]
XP_022978904.15.2e-8598.05endonuclease 4-like [Cucurbita maxima][more]
XP_022142132.13.5e-7387.01endonuclease 4-like [Momordica charantia][more]
XP_016902623.17.3e-7181.94PREDICTED: endonuclease 4-like isoform X3 [Cucumis melo][more]
BLAST of Carg13751 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G21585.11.7e-5363.45endonuclease 4[more]
AT4G21600.12.8e-4860.14endonuclease 5[more]
AT4G21590.12.0e-4657.14endonuclease 3[more]
AT1G68290.15.1e-4256.83endonuclease 2[more]
AT1G11190.15.6e-4151.70bifunctional nuclease i[more]
BLAST of Carg13751 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|F4JJL0|ENDO4_ARATH3.0e-5263.45Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=3 SV=1[more]
sp|F4JJL3|ENDO5_ARATH5.0e-4760.14Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=2 SV=1[more]
sp|Q8LDW6|ENDO3_ARATH3.6e-4557.14Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=2 SV=1[more]
sp|Q9C9G4|ENDO2_ARATH9.1e-4156.83Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1[more]
sp|Q9SXA6|ENDO1_ARATH1.0e-3951.70Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1[more]
BLAST of Carg13751 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S4E326|A0A1S4E326_CUCME4.8e-7181.94endonuclease 4-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103500060 PE=4 SV=1[more]
tr|A0A1S3CF84|A0A1S3CF84_CUCME1.4e-7081.82endonuclease 4-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103500060 PE=4 SV=1[more]
tr|A0A1S3CEU3|A0A1S3CEU3_CUCME1.4e-7081.82endonuclease 4-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500060 PE=4 SV=1[more]
tr|A0A0A0LY83|A0A0A0LY83_CUCSA5.0e-6880.52Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G163110 PE=4 SV=1[more]
tr|A0A2I4DJK4|A0A2I4DJK4_9ROSI2.4e-6275.32endonuclease 4-like OS=Juglans regia OX=51240 GN=LOC108980784 PE=4 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016788hydrolase activity, acting on ester bonds
GO:0003676nucleic acid binding
GO:0004519endonuclease activity
Vocabulary: Biological Process
TermDefinition
GO:0006308DNA catabolic process
Vocabulary: INTERPRO
TermDefinition
IPR008947PLipase_C/P1_nuclease_dom_sf
IPR003154S1/P1nuclease
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006308 DNA catabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13751-RACarg13751-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003154S1/P1 nucleasePFAMPF02265S1-P1_nucleasecoord: 25..154
e-value: 1.2E-35
score: 123.5
IPR003154S1/P1 nucleasePANTHERPTHR33146FAMILY NOT NAMEDcoord: 11..155
IPR003154S1/P1 nucleaseCDDcd11010S1-P1_nucleasecoord: 25..154
e-value: 3.25126E-42
score: 141.753
IPR008947Phospholipase C/P1 nuclease domain superfamilyGENE3DG3DSA:1.10.575.10coord: 25..157
e-value: 1.0E-47
score: 165.1
IPR008947Phospholipase C/P1 nuclease domain superfamilySUPERFAMILYSSF48537Phospholipase C/P1 nucleasecoord: 25..154
NoneNo IPR availablePANTHERPTHR33146:SF2ENDONUCLEASE 2coord: 11..155

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg13751Cla019463Watermelon (97103) v1carwmB0930
Carg13751Cla001356Watermelon (97103) v1carwmB0932
Carg13751ClCG01G022610Watermelon (Charleston Gray)carwcgB0907
Carg13751ClCG03G005590Watermelon (Charleston Gray)carwcgB0908
Carg13751CSPI01G11700Wild cucumber (PI 183967)carcpiB0968
Carg13751CmaCh03G004840Cucurbita maxima (Rimu)carcmaB1276
Carg13751CmaCh13G006720Cucurbita maxima (Rimu)carcmaB1274
Carg13751CmoCh13G006920Cucurbita moschata (Rifu)carcmoB1244
Carg13751CmoCh03G005030Cucurbita moschata (Rifu)carcmoB1246
Carg13751Lsi02G007090Bottle gourd (USVL1VR-Ls)carlsiB848
Carg13751Lsi01G003480Bottle gourd (USVL1VR-Ls)carlsiB845
Carg13751Cp4.1LG10g09290Cucurbita pepo (Zucchini)carcpeB1288
Carg13751Cp4.1LG20g04040Cucurbita pepo (Zucchini)carcpeB1291
Carg13751MELO3C002562.2Melon (DHL92) v3.6.1carmedB0921
Carg13751CsaV3_1G011850Cucumber (Chinese Long) v3carcucB0969
Carg13751Cla97C03G056900Watermelon (97103) v2carwmbB0976
Carg13751Bhi08G001562Wax gourdcarwgoB1077
Carg13751Bhi03G000364Wax gourdcarwgoB1074
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Carg13751Silver-seed gourdcarcarB037
Carg13751Silver-seed gourdcarcarB563
Carg13751Silver-seed gourdcarcarB637
Carg13751Cucumber (Gy14) v2carcgybB0922
Carg13751Cucumber (Gy14) v1carcgyB1047
Carg13751Cucumber (Gy14) v1carcgyB1048
Carg13751Cucurbita maxima (Rimu)carcmaB1279
Carg13751Cucurbita moschata (Rifu)carcmoB1245
Carg13751Cucurbita moschata (Rifu)carcmoB1250
Carg13751Cucurbita pepo (Zucchini)carcpeB1289
Carg13751Wild cucumber (PI 183967)carcpiB0971
Carg13751Wild cucumber (PI 183967)carcpiB0972
Carg13751Cucumber (Chinese Long) v2carcuB0948
Carg13751Cucumber (Chinese Long) v2carcuB0949
Carg13751Cucumber (Chinese Long) v3carcucB0972
Carg13751Cucumber (Chinese Long) v3carcucB0973
Carg13751Bottle gourd (USVL1VR-Ls)carlsiB846
Carg13751Melon (DHL92) v3.5.1carmeB0917
Carg13751Melon (DHL92) v3.5.1carmeB0918
Carg13751Melon (DHL92) v3.6.1carmedB0924
Carg13751Melon (DHL92) v3.6.1carmedB0925
Carg13751Watermelon (Charleston Gray)carwcgB0906
Carg13751Watermelon (97103) v1carwmB0931
Carg13751Watermelon (97103) v2carwmbB0972
Carg13751Watermelon (97103) v2carwmbB0974
Carg13751Wax gourdcarwgoB1075