Carg13625 (gene) Silver-seed gourd

NameCarg13625
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionLipase
LocationCucurbita_argyrosperma_scaffold_053 : 594053 .. 596230 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGTCGACCTGCTGCTGAAATTGGCATTCGAAGCAGCCTAGGAACTTTCTTGTTCGATTATGGAATCTTGTCGCCGATGAACACTAAGGCTTTTCCCTTGAGCGAAATGTTAATTTGACAATGGAACGACTTGTCGGCCCCACTAGATGCCCTCGATCAATGGCTTCTGATGGGCGGAAGCGGATAGCAATTCTTGTGATTGCTCAATTCCTGTTTGTTTCTGCTCCTATTCAGCGTCGATATAAAATTCCTAAACTCCTGTGTTACACCATGAATCAAAATGAACTCGCCCGATCCCTTCAATCCGATTGTTCTTTTACTTCTGCTTTGTGGATTCATAATAGTTTCTGCATTGGAACCTGGCTCAGTCGATGGTATCTGTAAATCGTTAGTGGAGCCTCACAACTACGCTTGCGAAGAACATCTAGTAATATAGCGACTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTTATGGAAATACCTAATTGATTCACAGAATGATCTTCCATTTGGGGTTTTCAGGTGATTACAAACGATGGGTTTATACTCAGCATGCAGAGAATCCCATCGGGAGGACGGACGAGTTCAGCCAATGGGCCTCCAGTTCTGCTACAACATGGTCTTCTAATGGTTGGTAATTTTGATTTTGGTGCCGTAATTTCTTGTGAAAGGATGATGTGTTTATGGATTGGTTTTACGAACTTTTTGTGATGGGAAGGATGCGGCAACATGGCTGTTGCTTCCTCCAGAAAGTTCTTTAGCATTCGTTTTGGCAGATAAAGGATTTGATGTGTGGCTTGCCAACACCCGTGGAACTAAATTTAGCCAAGGACATACCTCACTAGGCCCTGATGATCCTGTAATTGCTTGTTTCTCTTGCTTCCATTTTGATTTGCTTGCTGCTGAAATTGATATTGAGTTGTTCTTTAGGGCTTCTGGGATTGGTCATGGGATGAATTGGTAGCTTTTGATCTCCCTGCCACTCTCCAATATGTTCATAATCGTACTGGGCAGAATATGCACTATGTTGGACACTCATTGGTTAGTTTAAGCATTGCATTTTCTTTGCATCTTCGTGTTAGAGTATGTTTATCTGTGTCTGAGATGGAGAAGTTCTTGAATTGAAGGGAACATTGATTGCTCTTGCTGCATTTTCCAAACACCAATTGCTTGACATGTTGATATCAGCTGCTTTGATTAGCCCAATTGCCCATTTGGGTGATGTAACCTCTCCAATTGCGAGAAACGCTGCTGATAACTTTCTCGGTGAGGTACAATATTCGATCCTCTGTATATTCGTATTACTGTCGAGTTATGTCGTGTTGCCGATTCGTTTTATCCGTCGTAACAGGTCTTGTTCTGGTTGGGTGTCAAAGAGTTTGATCCAAGAGGGTAACTATTTGACCATTTCTCTTTTGTTAAAGGAAACTATTAGATTAGTGAAACTAGTTCATTTAGTTCTGTACTTACTTTGAATGAACAAATAGGAAGGCTGCAGTTCAGCTTCTTGTTGAAGTCTGTGCAAAGCCAGGAGTTGATTGCATCAACTTGTTGACATCTTTCACAGGTTCGAACGCTGTTTTAGTTCATTGTTTTATTTTCCAACCGAGCTCGTAGAGAGAATTATCGCTATTTTGTTTCGATTATAGGCCAGAATTGCTGTCTCAATCCATCTGTATCTCAAAACTTTCTAATCCATGAGCCTCAGCCAACGGCAACAAAGAACATGATCCATTTGTCTCAGAGTAAGGATCAAGGCTCTTCTGAGTATATTTGAATCTTTGTTGTTGTTTTGTGCGGTCTAAATATTATTTTGGGGATTATGGGCAGTGATCAGAAGTGGAACCATAGCAATGTACGACTACGTTGACGTGATCGAAAACATTAAACACTATGGGCAATCGACTCCTCCAACGTACAATATGACAAGCATTCCTAATGACTTCCCTCTCTTTCTTAGCTATGGAGGTGCTGATGCCTTGTCTGATGTCAACGATGTGCAACTCTTGTTGGATAACTTCAAAGATCACGACGGAGATAAGCTCGTCGTTCAGTTTAGAGAAGATTATGCTCATGCTGATTTTGTCATGGGAGAAAACGCTAAGCAAGTTGTGTATGATCCTCTTATCGCTTTCTTTATGCTTCAATGA

mRNA sequence

GAAGTCGACCTGCTGCTGAAATTGGCATTCGAAGCAGCCTAGGAACTTTCTTGTTCGATTATGGAATCTTGTCGCCGATGAACACTAAGGCTTTTCCCTTGAGCGAAATGTTAATTTGACAATGGAACGACTTGTCGGCCCCACTAGATGCCCTCGATCAATGGCTTCTGATGGGCGGAAGCGGATAGCAATTCTTGTGATTGCTCAATTCCTGTTTGTTTCTGCTCCTATTCAGCGTCGATATAAAATTCCTAAACTCCTGTGTTACACCATGAATCAAAATGAACTCGCCCGATCCCTTCAATCCGATTGTTCTTTTACTTCTGCTTTGTGGATTCATAATAGTTTCTGCATTGGAACCTGGCTCAGTCGATGGTATCTGTAAATCGTTAGTGGAGCCTCACAACTACGCTTGCGAAGAACATCTAGTGATTACAAACGATGGGTTTATACTCAGCATGCAGAGAATCCCATCGGGAGGACGGACGAGTTCAGCCAATGGGCCTCCAGTTCTGCTACAACATGGTCTTCTAATGGATGCGGCAACATGGCTGTTGCTTCCTCCAGAAAGTTCTTTAGCATTCGTTTTGGCAGATAAAGGATTTGATGTGTGGCTTGCCAACACCCGTGGAACTAAATTTAGCCAAGGACATACCTCACTAGGCCCTGATGATCCTGGCTTCTGGGATTGGTCATGGGATGAATTGGTAGCTTTTGATCTCCCTGCCACTCTCCAATATGTTCATAATCGTACTGGGCAGAATATGCACTATGTTGGACACTCATTGGGAACATTGATTGCTCTTGCTGCATTTTCCAAACACCAATTGCTTGACATGTTGATATCAGCTGCTTTGATTAGCCCAATTGCCCATTTGGGTGATGTAACCTCTCCAATTGCGAGAAACGCTGCTGATAACTTTCTCGGTGAGGTCTTGTTCTGGTTGGGTGTCAAAGAGTTTGATCCAAGAGGGAAGGCTGCAGTTCAGCTTCTTGTTGAAGTCTGTGCAAAGCCAGGAGTTGATTGCATCAACTTGTTGACATCTTTCACAGGCCAGAATTGCTGTCTCAATCCATCTGTATCTCAAAACTTTCTAATCCATGAGCCTCAGCCAACGGCAACAAAGAACATGATCCATTTGTCTCAGATGATCAGAAGTGGAACCATAGCAATGTACGACTACGTTGACGTGATCGAAAACATTAAACACTATGGGCAATCGACTCCTCCAACGTACAATATGACAAGCATTCCTAATGACTTCCCTCTCTTTCTTAGCTATGGAGGTGCTGATGCCTTGTCTGATGTCAACGATGTGCAACTCTTGTTGGATAACTTCAAAGATCACGACGGAGATAAGCTCGTCGTTCAGTTTAGAGAAGATTATGCTCATGCTGATTTTGTCATGGGAGAAAACGCTAAGCAAGTTGTGTATGATCCTCTTATCGCTTTCTTTATGCTTCAATGA

Coding sequence (CDS)

ATGAACTCGCCCGATCCCTTCAATCCGATTGTTCTTTTACTTCTGCTTTGTGGATTCATAATAGTTTCTGCATTGGAACCTGGCTCAGTCGATGGTATCTGTAAATCGTTAGTGGAGCCTCACAACTACGCTTGCGAAGAACATCTAGTGATTACAAACGATGGGTTTATACTCAGCATGCAGAGAATCCCATCGGGAGGACGGACGAGTTCAGCCAATGGGCCTCCAGTTCTGCTACAACATGGTCTTCTAATGGATGCGGCAACATGGCTGTTGCTTCCTCCAGAAAGTTCTTTAGCATTCGTTTTGGCAGATAAAGGATTTGATGTGTGGCTTGCCAACACCCGTGGAACTAAATTTAGCCAAGGACATACCTCACTAGGCCCTGATGATCCTGGCTTCTGGGATTGGTCATGGGATGAATTGGTAGCTTTTGATCTCCCTGCCACTCTCCAATATGTTCATAATCGTACTGGGCAGAATATGCACTATGTTGGACACTCATTGGGAACATTGATTGCTCTTGCTGCATTTTCCAAACACCAATTGCTTGACATGTTGATATCAGCTGCTTTGATTAGCCCAATTGCCCATTTGGGTGATGTAACCTCTCCAATTGCGAGAAACGCTGCTGATAACTTTCTCGGTGAGGTCTTGTTCTGGTTGGGTGTCAAAGAGTTTGATCCAAGAGGGAAGGCTGCAGTTCAGCTTCTTGTTGAAGTCTGTGCAAAGCCAGGAGTTGATTGCATCAACTTGTTGACATCTTTCACAGGCCAGAATTGCTGTCTCAATCCATCTGTATCTCAAAACTTTCTAATCCATGAGCCTCAGCCAACGGCAACAAAGAACATGATCCATTTGTCTCAGATGATCAGAAGTGGAACCATAGCAATGTACGACTACGTTGACGTGATCGAAAACATTAAACACTATGGGCAATCGACTCCTCCAACGTACAATATGACAAGCATTCCTAATGACTTCCCTCTCTTTCTTAGCTATGGAGGTGCTGATGCCTTGTCTGATGTCAACGATGTGCAACTCTTGTTGGATAACTTCAAAGATCACGACGGAGATAAGCTCGTCGTTCAGTTTAGAGAAGATTATGCTCATGCTGATTTTGTCATGGGAGAAAACGCTAAGCAAGTTGTGTATGATCCTCTTATCGCTTTCTTTATGCTTCAATGA

Protein sequence

MNSPDPFNPIVLLLLLCGFIIVSALEPGSVDGICKSLVEPHNYACEEHLVITNDGFILSMQRIPSGGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFDVWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSLGTLIALAAFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAAVQLLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQMIRSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLDNFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ
BLAST of Carg13625 vs. NCBI nr
Match: XP_008456397.1 (PREDICTED: triacylglycerol lipase 2-like [Cucumis melo])

HSP 1 Score: 728.0 bits (1878), Expect = 1.7e-206
Identity = 357/399 (89.47%), Postives = 371/399 (92.98%), Query Frame = 0

Query: 1   MNSPDPFNPIVLLLLLCGFI----IVSALEPGSVDGICKSLVEPHNYACEEHLVITNDGF 60
           M SP+ FN IVLLLL  G       VSALEPGSV+GICKSLVEPH++ACEEHLVIT DGF
Sbjct: 1   MYSPNTFNSIVLLLLFYGLAGGIKRVSALEPGSVNGICKSLVEPHDFACEEHLVITTDGF 60

Query: 61  ILSMQRIPSGGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFDVWLANTR 120
           ILSMQRIPS GRT+SANGPPVLLQHGLLMDAATWL+LPPESSLAFVLADKGFDVWLANTR
Sbjct: 61  ILSMQRIPS-GRTNSANGPPVLLQHGLLMDAATWLMLPPESSLAFVLADKGFDVWLANTR 120

Query: 121 GTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSLGTLIALA 180
           GTKFSQGH+SLGPDDPGFWDWSWDELVAFDLPATLQYVH+ TGQ MHYVGHSLGTLIALA
Sbjct: 121 GTKFSQGHSSLGPDDPGFWDWSWDELVAFDLPATLQYVHDHTGQKMHYVGHSLGTLIALA 180

Query: 181 AFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAAVQ 240
           AFSKHQLLDML SAALISPIAHLG VTSPIARNAADNFLGEVLFWLGVKEFDPRGKA VQ
Sbjct: 181 AFSKHQLLDMLRSAALISPIAHLGKVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAGVQ 240

Query: 241 LLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQMIRSGTI 300
           LLV+VCAKPGVDC+NLLTSFTGQNCCLNPSVSQ FL HEPQPTATKNMIHLSQMIRSGTI
Sbjct: 241 LLVDVCAKPGVDCVNLLTSFTGQNCCLNPSVSQIFLTHEPQPTATKNMIHLSQMIRSGTI 300

Query: 301 AMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLDNFKDH 360
           +MYDYVDVI+NIKHYGQ TPP YNM SIP DFPLFL+YGGADALSDVNDVQLLLDN KDH
Sbjct: 301 SMYDYVDVIQNIKHYGQPTPPEYNMASIPTDFPLFLTYGGADALSDVNDVQLLLDNLKDH 360

Query: 361 DGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ 396
           DGDKLVVQFREDYAHADFVMG+NAKQ VYDPLIAFF LQ
Sbjct: 361 DGDKLVVQFREDYAHADFVMGKNAKQAVYDPLIAFFNLQ 398

BLAST of Carg13625 vs. NCBI nr
Match: XP_004143194.1 (PREDICTED: triacylglycerol lipase 2-like [Cucumis sativus] >KGN47007.1 hypothetical protein Csa_6G168240 [Cucumis sativus])

HSP 1 Score: 725.7 bits (1872), Expect = 8.4e-206
Identity = 355/399 (88.97%), Postives = 368/399 (92.23%), Query Frame = 0

Query: 1   MNSPDPFNPIVLLLLLCGFI----IVSALEPGSVDGICKSLVEPHNYACEEHLVITNDGF 60
           M SP+ FN IV  LL CG       VSALEPGSV+GICKSLVEPH++ACEEHLVIT DGF
Sbjct: 1   MYSPNTFNSIVFSLLFCGLAGGIKRVSALEPGSVNGICKSLVEPHDFACEEHLVITTDGF 60

Query: 61  ILSMQRIPSGGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFDVWLANTR 120
           ILSMQRIPS  RT+SANGPPVLLQHGLLMDAATWL+LPPESSLAFVLADKGFDVWLANTR
Sbjct: 61  ILSMQRIPS-RRTNSANGPPVLLQHGLLMDAATWLMLPPESSLAFVLADKGFDVWLANTR 120

Query: 121 GTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSLGTLIALA 180
           GTKFSQGH+SLGPDDPGFWDWSWDELVAFDLPATLQYVH+ TGQ MHYVGHSLGTL ALA
Sbjct: 121 GTKFSQGHSSLGPDDPGFWDWSWDELVAFDLPATLQYVHDHTGQKMHYVGHSLGTLTALA 180

Query: 181 AFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAAVQ 240
           AFSKHQLLDML SAALISPIAHLG VTSPIARNAADNFLGEVLFWLGVKEFDPRGKA +Q
Sbjct: 181 AFSKHQLLDMLRSAALISPIAHLGKVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAGIQ 240

Query: 241 LLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQMIRSGTI 300
           LLVEVCAKPGVDC+NLLTSFTGQNCCLNPSVSQ FL HEPQPTATKNMIHLSQMIRSGTI
Sbjct: 241 LLVEVCAKPGVDCVNLLTSFTGQNCCLNPSVSQIFLTHEPQPTATKNMIHLSQMIRSGTI 300

Query: 301 AMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLDNFKDH 360
           +MYDYVDVI+NIKHYGQ TPP YNM SIP DFPLFL+YGGADALSDVNDVQLLLDN KDH
Sbjct: 301 SMYDYVDVIQNIKHYGQPTPPEYNMASIPTDFPLFLTYGGADALSDVNDVQLLLDNLKDH 360

Query: 361 DGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ 396
           DGDKLVVQFREDYAHADFVMGENAKQ VYDPLIAFF LQ
Sbjct: 361 DGDKLVVQFREDYAHADFVMGENAKQAVYDPLIAFFNLQ 398

BLAST of Carg13625 vs. NCBI nr
Match: XP_022947370.1 (triacylglycerol lipase 2-like [Cucurbita moschata])

HSP 1 Score: 713.0 bits (1839), Expect = 5.7e-202
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 50  VITNDGFILSMQRIPSGGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFD 109
           VITNDGFILSMQRIPSGGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFD
Sbjct: 159 VITNDGFILSMQRIPSGGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFD 218

Query: 110 VWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSL 169
           VWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSL
Sbjct: 219 VWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSL 278

Query: 170 GTLIALAAFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDP 229
           GTLIALAAFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDP
Sbjct: 279 GTLIALAAFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDP 338

Query: 230 RGKAAVQLLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQ 289
           RGKAAVQLLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQ
Sbjct: 339 RGKAAVQLLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQ 398

Query: 290 MIRSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLL 349
           MIRSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLL
Sbjct: 399 MIRSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLL 458

Query: 350 LDNFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ 396
           LDNFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ
Sbjct: 459 LDNFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ 504

BLAST of Carg13625 vs. NCBI nr
Match: XP_022970785.1 (triacylglycerol lipase 2-like [Cucurbita maxima])

HSP 1 Score: 706.1 bits (1821), Expect = 6.9e-200
Identity = 345/346 (99.71%), Postives = 345/346 (99.71%), Query Frame = 0

Query: 50  VITNDGFILSMQRIPSGGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFD 109
           VITNDGFILSMQRIPS GRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFD
Sbjct: 159 VITNDGFILSMQRIPS-GRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFD 218

Query: 110 VWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSL 169
           VWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSL
Sbjct: 219 VWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSL 278

Query: 170 GTLIALAAFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDP 229
           GTLIALAAFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDP
Sbjct: 279 GTLIALAAFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDP 338

Query: 230 RGKAAVQLLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQ 289
           RGKAAVQLLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQ
Sbjct: 339 RGKAAVQLLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQ 398

Query: 290 MIRSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLL 349
           MIRSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLL
Sbjct: 399 MIRSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLL 458

Query: 350 LDNFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ 396
           LDNFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ
Sbjct: 459 LDNFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ 503

BLAST of Carg13625 vs. NCBI nr
Match: XP_023533928.1 (triacylglycerol lipase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 701.4 bits (1809), Expect = 1.7e-198
Identity = 341/344 (99.13%), Postives = 343/344 (99.71%), Query Frame = 0

Query: 52  TNDGFILSMQRIPSGGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFDVW 111
           TNDGFILSMQRIPS GRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFDVW
Sbjct: 161 TNDGFILSMQRIPS-GRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFDVW 220

Query: 112 LANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSLGT 171
           LANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSLGT
Sbjct: 221 LANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSLGT 280

Query: 172 LIALAAFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDPRG 231
           LIALAAFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDPRG
Sbjct: 281 LIALAAFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDPRG 340

Query: 232 KAAVQLLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQMI 291
           KAA+QLLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQMI
Sbjct: 341 KAAIQLLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQMI 400

Query: 292 RSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLD 351
           RSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFL+YGGADALSDVNDVQLLLD
Sbjct: 401 RSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLTYGGADALSDVNDVQLLLD 460

Query: 352 NFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ 396
           NFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ
Sbjct: 461 NFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ 503

BLAST of Carg13625 vs. TAIR10
Match: AT5G14180.1 (Myzus persicae-induced lipase 1)

HSP 1 Score: 405.2 bits (1040), Expect = 4.6e-113
Identity = 191/368 (51.90%), Postives = 253/368 (68.75%), Query Frame = 0

Query: 32  GICKSLVEPHNYACEEHLVITNDGFILSMQRIPSG--GRTSSANG--PPVLLQHGLLMDA 91
           GIC S V    Y CEEH V+T DG+IL+MQRIP G  G  +   G   PVL+QHG+L+D 
Sbjct: 50  GICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDG 109

Query: 92  ATWLLLPPESSLAFVLADKGFDVWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDL 151
            +WLL P + +L  +LAD+GFDVW+ NTRGT+FS+ H  L P    FW+W+WDELV++DL
Sbjct: 110 MSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDL 169

Query: 152 PATLQYVHNRTGQNMHYVGHSLGTLIALAAFSKHQLLDMLISAALISPIAHLGDVTSPIA 211
           PA   ++H  TGQ +HY+GHSLGTLI  A+FS+  L+D + SAA++SP+A+L  +T+ I 
Sbjct: 170 PAMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVIG 229

Query: 212 RNAADNFLGEVLFWLGVKEFDPRGKAAVQLLVEVCAKPGVDCINLLTSFTGQNCCLNPSV 271
             AA  FL E    LG  EF+P+       +  +C K G+DC +L++  TG+NCCLN S 
Sbjct: 230 DIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNAST 289

Query: 272 SQNFLIHEPQPTATKNMIHLSQMIRSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPND 331
              FL +EPQ T+TKNMIHL+Q +R   +  Y+Y     NIKHYGQ+ PP YN+++IP++
Sbjct: 290 IDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPHE 349

Query: 332 FPLFLSYGGADALSDVNDVQLLLDNFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDP 391
            PLF SYGG D+L+DV DV+ LLD FK HD DK+ VQF +DYAHADF+MG  AK VVY+ 
Sbjct: 350 LPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNVQFVKDYAHADFIMGVTAKDVVYNQ 409

Query: 392 LIAFFMLQ 396
           +  FF  Q
Sbjct: 410 VATFFKRQ 417

BLAST of Carg13625 vs. TAIR10
Match: AT2G15230.1 (lipase 1)

HSP 1 Score: 315.1 bits (806), Expect = 6.2e-86
Identity = 160/389 (41.13%), Postives = 235/389 (60.41%), Query Frame = 0

Query: 11  VLLLLLCGFIIVSA------LEPGSVDGICKSLVEPHNYACEEHLVITNDGFILSMQRIP 70
           +L+ +L    I SA      L    V+ +C  L+ P NY+C EH + T DG+IL++QR+ 
Sbjct: 4   LLVAVLTSLTIFSALTQSHLLHGSPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVA 63

Query: 71  S-GGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFDVWLANTRGTKFSQG 130
           S G R  S  GPPVLLQHGL M    W L  P+ SL F+LAD GFDVW+ N RGT++S G
Sbjct: 64  SLGPRLQS--GPPVLLQHGLFMAGDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYG 123

Query: 131 HTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSLGTLIALAAFSKHQL 190
           H +L   D  FWDWSW +L  +DL   +QY+++ +   +  VGHS GT+++ AA ++  +
Sbjct: 124 HVTLSDTDKEFWDWSWQDLAMYDLAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHV 183

Query: 191 LDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAAVQLLVEVCA 250
            +M+ +AAL+ PI++L  VT+P+        L +++  LG+ + + R    V+L+  +C 
Sbjct: 184 AEMVEAAALLCPISYLDHVTAPLVERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLC- 243

Query: 251 KPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQMIRSGTIAMYDYVD 310
           +  +DC + LTS TG NCC N S  + +L +EP P++ KN+ HL QMIR GT A YDY  
Sbjct: 244 EGHMDCTDFLTSITGTNCCFNASKIEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDY-G 303

Query: 311 VIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLDNFKDHDGDKLVV 370
             +N++ YG S PP + ++ IP   P+++ YGG D L+DV DV+  L            +
Sbjct: 304 YFKNLRTYGLSKPPEFILSHIPASLPMWMGYGGTDGLADVTDVEHTLAELPSSP----EL 363

Query: 371 QFREDYAHADFVMGENAKQVVYDPLIAFF 393
            + EDY H DFV+G +AK+ VY  +I FF
Sbjct: 364 LYLEDYGHIDFVLGSSAKEDVYKHMIQFF 384

BLAST of Carg13625 vs. TAIR10
Match: AT1G73920.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 71.6 bits (174), Expect = 1.2e-12
Identity = 40/122 (32.79%), Postives = 63/122 (51.64%), Query Frame = 0

Query: 34  CKSLVEPHNYACEEHLVITNDGFILSMQRIPSGGRTSSANGPPVLLQHGLLMDAATWLLL 93
           C+ ++    Y  E   VIT+DG++L ++RIP      +     V LQHG+L  +  W+  
Sbjct: 296 CQDVITELGYPYEAIRVITSDGYVLVLERIPRRDARKA-----VFLQHGVLDSSMGWVSN 355

Query: 94  PPESSLAFVLADKGFDVWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQY 153
               S AF   D+G+DV+L N RG   S+ H +       FW +S +E    D+PA ++ 
Sbjct: 356 GVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINEHGTEDIPAMIEK 411

Query: 154 VH 156
           +H
Sbjct: 416 IH 411

BLAST of Carg13625 vs. TAIR10
Match: AT1G18460.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 68.6 bits (166), Expect = 1.0e-11
Identity = 38/122 (31.15%), Postives = 61/122 (50.00%), Query Frame = 0

Query: 34  CKSLVEPHNYACEEHLVITNDGFILSMQRIPSGGRTSSANGPPVLLQHGLLMDAATWLLL 93
           C+ ++    Y  E   V+T+DG+ L ++RIP      +     V LQHG++  +  W+  
Sbjct: 294 CQDVITELGYPYEAIRVVTSDGYGLLLERIPRRDARKA-----VYLQHGVMDSSMGWVSN 353

Query: 94  PPESSLAFVLADKGFDVWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQY 153
               S AF   D+G+DV+L N RG   S+ H         FW +S +E    D+PA ++ 
Sbjct: 354 GVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVKKNISSKDFWRYSINEHATEDIPAMIEK 409

Query: 154 VH 156
           +H
Sbjct: 414 IH 409

BLAST of Carg13625 vs. Swiss-Prot
Match: sp|Q67ZU1|LIP2_ARATH (Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 8.2e-112
Identity = 191/368 (51.90%), Postives = 253/368 (68.75%), Query Frame = 0

Query: 32  GICKSLVEPHNYACEEHLVITNDGFILSMQRIPSG--GRTSSANG--PPVLLQHGLLMDA 91
           GIC S V    Y CEEH V+T DG+IL+MQRIP G  G  +   G   PVL+QHG+L+D 
Sbjct: 50  GICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDG 109

Query: 92  ATWLLLPPESSLAFVLADKGFDVWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDL 151
            +WLL P + +L  +LAD+GFDVW+ NTRGT+FS+ H  L P    FW+W+WDELV++DL
Sbjct: 110 MSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDL 169

Query: 152 PATLQYVHNRTGQNMHYVGHSLGTLIALAAFSKHQLLDMLISAALISPIAHLGDVTSPIA 211
           PA   ++H  TGQ +HY+GHSLGTLI  A+FS+  L+D + SAA++SP+A+L  +T+ I 
Sbjct: 170 PAMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVIG 229

Query: 212 RNAADNFLGEVLFWLGVKEFDPRGKAAVQLLVEVCAKPGVDCINLLTSFTGQNCCLNPSV 271
             AA  FL E    LG  EF+P+       +  +C K G+DC +L++  TG+NCCLN S 
Sbjct: 230 DIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNAST 289

Query: 272 SQNFLIHEPQPTATKNMIHLSQMIRSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPND 331
              FL +EPQ T+TKNMIHL+Q +R   +  Y+Y     NIKHYGQ+ PP YN+++IP++
Sbjct: 290 IDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPHE 349

Query: 332 FPLFLSYGGADALSDVNDVQLLLDNFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDP 391
            PLF SYGG D+L+DV DV+ LLD FK HD DK+ VQF +DYAHADF+MG  AK VVY+ 
Sbjct: 350 LPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNVQFVKDYAHADFIMGVTAKDVVYNQ 409

Query: 392 LIAFFMLQ 396
           +  FF  Q
Sbjct: 410 VATFFKRQ 417

BLAST of Carg13625 vs. Swiss-Prot
Match: sp|Q71DJ5|LIP1_ARATH (Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.1e-84
Identity = 160/389 (41.13%), Postives = 235/389 (60.41%), Query Frame = 0

Query: 11  VLLLLLCGFIIVSA------LEPGSVDGICKSLVEPHNYACEEHLVITNDGFILSMQRIP 70
           +L+ +L    I SA      L    V+ +C  L+ P NY+C EH + T DG+IL++QR+ 
Sbjct: 4   LLVAVLTSLTIFSALTQSHLLHGSPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVA 63

Query: 71  S-GGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFDVWLANTRGTKFSQG 130
           S G R  S  GPPVLLQHGL M    W L  P+ SL F+LAD GFDVW+ N RGT++S G
Sbjct: 64  SLGPRLQS--GPPVLLQHGLFMAGDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYG 123

Query: 131 HTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSLGTLIALAAFSKHQL 190
           H +L   D  FWDWSW +L  +DL   +QY+++ +   +  VGHS GT+++ AA ++  +
Sbjct: 124 HVTLSDTDKEFWDWSWQDLAMYDLAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHV 183

Query: 191 LDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAAVQLLVEVCA 250
            +M+ +AAL+ PI++L  VT+P+        L +++  LG+ + + R    V+L+  +C 
Sbjct: 184 AEMVEAAALLCPISYLDHVTAPLVERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLC- 243

Query: 251 KPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQMIRSGTIAMYDYVD 310
           +  +DC + LTS TG NCC N S  + +L +EP P++ KN+ HL QMIR GT A YDY  
Sbjct: 244 EGHMDCTDFLTSITGTNCCFNASKIEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDY-G 303

Query: 311 VIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLDNFKDHDGDKLVV 370
             +N++ YG S PP + ++ IP   P+++ YGG D L+DV DV+  L            +
Sbjct: 304 YFKNLRTYGLSKPPEFILSHIPASLPMWMGYGGTDGLADVTDVEHTLAELPSSP----EL 363

Query: 371 QFREDYAHADFVMGENAKQVVYDPLIAFF 393
            + EDY H DFV+G +AK+ VY  +I FF
Sbjct: 364 LYLEDYGHIDFVLGSSAKEDVYKHMIQFF 384

BLAST of Carg13625 vs. Swiss-Prot
Match: sp|P80035|LIPG_CANLF (Gastric triacylglycerol lipase OS=Canis lupus familiaris OX=9615 GN=LIPF PE=1 SV=2)

HSP 1 Score: 219.2 bits (557), Expect = 8.4e-56
Identity = 131/358 (36.59%), Postives = 200/358 (55.87%), Query Frame = 0

Query: 43  YACEEHLVITNDGFILSMQRIPSGGRTSSANG--PPVLLQHGLLMDAATWLLLPPESSLA 102
           Y  EE+ V+T DG+IL + RIP G + S   G  P   LQHGLL  A  W+   P +SLA
Sbjct: 44  YPAEEYEVVTEDGYILGIDRIPYGRKNSENIGRRPVAFLQHGLLASATNWISNLPNNSLA 103

Query: 103 FVLADKGFDVWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQ 162
           F+LAD G+DVWL N+RG  +++ +    PD   FW +S+DE+  +DLPAT+ ++  +TGQ
Sbjct: 104 FILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFILKKTGQ 163

Query: 163 N-MHYVGHSLGTLIALAAFSKH-QLLDMLISAALISPIAHLGDVTSPIAR-NAADNFLGE 222
           + +HYVGHS GT I   AFS + +L   + +   ++P+A +    + + +     +FL +
Sbjct: 164 DKLHYVGHSQGTTIGFIAFSTNPKLAKRIKTFYALAPVATVKYTETLLNKLMLVPSFLFK 223

Query: 223 VLFWLGVKEFDPRGKAAVQLLVEVCAKPGVD--CINLLTSFTG-QNCCLNPSVSQNFLIH 282
           ++F  G K F P       L  EVC++  VD  C N L    G     LN S    +L H
Sbjct: 224 LIF--GNKIFYPHHFFDQFLATEVCSRETVDLLCSNALFIICGFDTMNLNMSRLDVYLSH 283

Query: 283 EPQPTATKNMIHLSQMIRSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSY 342
            P  T+ +N++H SQ ++SG    +D+   ++N+ HY QS PP YN+T +    P+ +  
Sbjct: 284 NPAGTSVQNVLHWSQAVKSGKFQAFDWGSPVQNMMHYHQSMPPYYNLTDM--HVPIAVWN 343

Query: 343 GGADALSDVNDVQLLLDNFKDHDGDKLVVQFRE--DYAHADFVMGENAKQVVYDPLIA 391
           GG D L+D +DV LLL    +      ++  R+   Y H DF+   +A Q VY+ +++
Sbjct: 344 GGNDLLADPHDVDLLLSKLPN------LIYHRKIPPYNHLDFIWAMDAPQAVYNEIVS 391

BLAST of Carg13625 vs. Swiss-Prot
Match: sp|Q29458|LIPG_BOVIN (Gastric triacylglycerol lipase OS=Bos taurus OX=9913 GN=LIPF PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.2e-55
Identity = 132/356 (37.08%), Postives = 194/356 (54.49%), Query Frame = 0

Query: 43  YACEEHLVITNDGFILSMQRIPSGGRTSSANG--PPVLLQHGLLMDAATWLLLPPESSLA 102
           Y  E H VIT DG+IL + RIP G   ++  G  P V LQHGLL  A  W+   P++SL 
Sbjct: 43  YPSEMHKVITADGYILQVYRIPHGKNNANHLGQRPVVFLQHGLLGSATNWISNLPKNSLG 102

Query: 103 FVLADKGFDVWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQ 162
           F+LAD G+DVWL N+RG  ++Q H    PD P FW +S+DE+  +DLP+T+ ++  RTGQ
Sbjct: 103 FLLADAGYDVWLGNSRGNTWAQEHLYYSPDSPEFWAFSFDEMAEYDLPSTIDFILRRTGQ 162

Query: 163 -NMHYVGHSLGTLIALAAFSKHQLLDMLISA-ALISPIAHLGDVTSPIARNA-ADNFLGE 222
             +HYVGHS GT I   AFS    L   I     ++P+A +    S   + A   +FL +
Sbjct: 163 KKLHYVGHSQGTTIGFIAFSTSPTLAEKIKVFYALAPVATVKYTKSLFNKLALIPHFLFK 222

Query: 223 VLFWLGVKEFDPRGKAAVQLLVEVCAKPGVD--CINLLTSFTG-QNCCLNPSVSQNFLIH 282
           ++F  G K F P       L VE+C++  +D  C N L + TG  N   N S    ++ H
Sbjct: 223 IIF--GDKMFYPHTFLEQFLGVEMCSRETLDVLCKNALFAITGVDNKNFNMSRLDVYIAH 282

Query: 283 EPQPTATKNMIHLSQMIRSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSY 342
            P  T+ +N +H  Q ++SG    +D+    +N+ HY Q TPP YN+T++  + P+ +  
Sbjct: 283 NPAGTSVQNTLHWRQAVKSGKFQAFDWGAPYQNLMHYHQPTPPIYNLTAM--NVPIAVWS 342

Query: 343 GGADALSDVNDVQLLLDNFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLIA 391
              D L+D  DV  LL    +    K +     +Y H DF+   +A Q VY+ +++
Sbjct: 343 ADNDLLADPQDVDFLLSKLSNLIYHKEI----PNYNHLDFIWAMDAPQEVYNEIVS 390

BLAST of Carg13625 vs. Swiss-Prot
Match: sp|P04634|LIPG_RAT (Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.2e-55
Identity = 131/356 (36.80%), Postives = 198/356 (55.62%), Query Frame = 0

Query: 43  YACEEHLVITNDGFILSMQRIPSGGRTSSANG--PPVLLQHGLLMDAATWLLLPPESSLA 102
           Y C+E+ V+T DG+IL + RIP G   S   G  P V LQHGL+  A  W+   P +SLA
Sbjct: 43  YPCQEYEVVTEDGYILGVYRIPHGKNNSENIGKRPVVYLQHGLIASATNWIANLPNNSLA 102

Query: 103 FVLADKGFDVWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQ 162
           F+LAD G+DVWL N+RG  +S+ +    PD   FW +S+DE+  +DLPAT+ ++  +TGQ
Sbjct: 103 FMLADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATINFIVQKTGQ 162

Query: 163 -NMHYVGHSLGTLIALAAFSKHQLLDMLISA-ALISPIAHLGDVTSPIAR-NAADNFLGE 222
             +HYVGHS GT I   AFS +  L   I     ++P+A +    SP+ + +    FL +
Sbjct: 163 EKIHYVGHSQGTTIGFIAFSTNPTLAKKIKTFYALAPVATVKYTQSPLKKISFIPTFLFK 222

Query: 223 VLFWLGVKEFDPRGKAAVQLLVEVCAKPGVD--CINLLTSFTG-QNCCLNPSVSQNFLIH 282
           ++F  G K F P       L  EVC++  +D  C N L  F G     LN S    +L H
Sbjct: 223 LMF--GKKMFLPHTYFDDFLGTEVCSREVLDLLCSNTLFIFCGFDKKNLNVSRFDVYLGH 282

Query: 283 EPQPTATKNMIHLSQMIRSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSY 342
            P  T+ ++ +H +Q++RSG    +++    +N+ HY Q TPP Y+++++    P+ +  
Sbjct: 283 NPAGTSVQDFLHWAQLVRSGKFQAFNWGSPSQNMLHYNQKTPPEYDVSAM--TVPVAVWN 342

Query: 343 GGADALSDVNDVQLLLDNFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLIA 391
           GG D L+D  DV +LL    +    K ++     Y H DF+   +A Q VY+ +I+
Sbjct: 343 GGNDILADPQDVAMLLPKLSNLLFHKEILA----YNHLDFIWAMDAPQEVYNEMIS 390

BLAST of Carg13625 vs. TrEMBL
Match: tr|A0A1S3C4E7|A0A1S3C4E7_CUCME (Lipase OS=Cucumis melo OX=3656 GN=LOC103496348 PE=3 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 1.1e-206
Identity = 357/399 (89.47%), Postives = 371/399 (92.98%), Query Frame = 0

Query: 1   MNSPDPFNPIVLLLLLCGFI----IVSALEPGSVDGICKSLVEPHNYACEEHLVITNDGF 60
           M SP+ FN IVLLLL  G       VSALEPGSV+GICKSLVEPH++ACEEHLVIT DGF
Sbjct: 1   MYSPNTFNSIVLLLLFYGLAGGIKRVSALEPGSVNGICKSLVEPHDFACEEHLVITTDGF 60

Query: 61  ILSMQRIPSGGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFDVWLANTR 120
           ILSMQRIPS GRT+SANGPPVLLQHGLLMDAATWL+LPPESSLAFVLADKGFDVWLANTR
Sbjct: 61  ILSMQRIPS-GRTNSANGPPVLLQHGLLMDAATWLMLPPESSLAFVLADKGFDVWLANTR 120

Query: 121 GTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSLGTLIALA 180
           GTKFSQGH+SLGPDDPGFWDWSWDELVAFDLPATLQYVH+ TGQ MHYVGHSLGTLIALA
Sbjct: 121 GTKFSQGHSSLGPDDPGFWDWSWDELVAFDLPATLQYVHDHTGQKMHYVGHSLGTLIALA 180

Query: 181 AFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAAVQ 240
           AFSKHQLLDML SAALISPIAHLG VTSPIARNAADNFLGEVLFWLGVKEFDPRGKA VQ
Sbjct: 181 AFSKHQLLDMLRSAALISPIAHLGKVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAGVQ 240

Query: 241 LLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQMIRSGTI 300
           LLV+VCAKPGVDC+NLLTSFTGQNCCLNPSVSQ FL HEPQPTATKNMIHLSQMIRSGTI
Sbjct: 241 LLVDVCAKPGVDCVNLLTSFTGQNCCLNPSVSQIFLTHEPQPTATKNMIHLSQMIRSGTI 300

Query: 301 AMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLDNFKDH 360
           +MYDYVDVI+NIKHYGQ TPP YNM SIP DFPLFL+YGGADALSDVNDVQLLLDN KDH
Sbjct: 301 SMYDYVDVIQNIKHYGQPTPPEYNMASIPTDFPLFLTYGGADALSDVNDVQLLLDNLKDH 360

Query: 361 DGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ 396
           DGDKLVVQFREDYAHADFVMG+NAKQ VYDPLIAFF LQ
Sbjct: 361 DGDKLVVQFREDYAHADFVMGKNAKQAVYDPLIAFFNLQ 398

BLAST of Carg13625 vs. TrEMBL
Match: tr|A0A0A0KF57|A0A0A0KF57_CUCSA (Lipase OS=Cucumis sativus OX=3659 GN=Csa_6G168240 PE=3 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 5.6e-206
Identity = 355/399 (88.97%), Postives = 368/399 (92.23%), Query Frame = 0

Query: 1   MNSPDPFNPIVLLLLLCGFI----IVSALEPGSVDGICKSLVEPHNYACEEHLVITNDGF 60
           M SP+ FN IV  LL CG       VSALEPGSV+GICKSLVEPH++ACEEHLVIT DGF
Sbjct: 1   MYSPNTFNSIVFSLLFCGLAGGIKRVSALEPGSVNGICKSLVEPHDFACEEHLVITTDGF 60

Query: 61  ILSMQRIPSGGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVLADKGFDVWLANTR 120
           ILSMQRIPS  RT+SANGPPVLLQHGLLMDAATWL+LPPESSLAFVLADKGFDVWLANTR
Sbjct: 61  ILSMQRIPS-RRTNSANGPPVLLQHGLLMDAATWLMLPPESSLAFVLADKGFDVWLANTR 120

Query: 121 GTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMHYVGHSLGTLIALA 180
           GTKFSQGH+SLGPDDPGFWDWSWDELVAFDLPATLQYVH+ TGQ MHYVGHSLGTL ALA
Sbjct: 121 GTKFSQGHSSLGPDDPGFWDWSWDELVAFDLPATLQYVHDHTGQKMHYVGHSLGTLTALA 180

Query: 181 AFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAAVQ 240
           AFSKHQLLDML SAALISPIAHLG VTSPIARNAADNFLGEVLFWLGVKEFDPRGKA +Q
Sbjct: 181 AFSKHQLLDMLRSAALISPIAHLGKVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAGIQ 240

Query: 241 LLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKNMIHLSQMIRSGTI 300
           LLVEVCAKPGVDC+NLLTSFTGQNCCLNPSVSQ FL HEPQPTATKNMIHLSQMIRSGTI
Sbjct: 241 LLVEVCAKPGVDCVNLLTSFTGQNCCLNPSVSQIFLTHEPQPTATKNMIHLSQMIRSGTI 300

Query: 301 AMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDVNDVQLLLDNFKDH 360
           +MYDYVDVI+NIKHYGQ TPP YNM SIP DFPLFL+YGGADALSDVNDVQLLLDN KDH
Sbjct: 301 SMYDYVDVIQNIKHYGQPTPPEYNMASIPTDFPLFLTYGGADALSDVNDVQLLLDNLKDH 360

Query: 361 DGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ 396
           DGDKLVVQFREDYAHADFVMGENAKQ VYDPLIAFF LQ
Sbjct: 361 DGDKLVVQFREDYAHADFVMGENAKQAVYDPLIAFFNLQ 398

BLAST of Carg13625 vs. TrEMBL
Match: tr|A0A2N9ETQ2|A0A2N9ETQ2_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS5826 PE=4 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 7.4e-166
Identity = 278/376 (73.94%), Postives = 319/376 (84.84%), Query Frame = 0

Query: 22  VSALEPGSVDGICKSLVEPHNYACEEHLVITNDGFILSMQRIPSG--GRTSSANGPPVLL 81
           +SAL P   DGIC S+VE   Y CEEH VIT DG+ILSMQRIP G  G+T   N PP+LL
Sbjct: 204 MSALAPVPNDGICTSMVETQGYVCEEHKVITQDGYILSMQRIPEGRSGKT-PGNQPPILL 263

Query: 82  QHGLLMDAATWLLLPPESSLAFVLADKGFDVWLANTRGTKFSQGHTSLGPDDPGFWDWSW 141
           QHGLLMD  TWLLLPP+ SLAF+LAD GFDVWL NTRGTK+S+GHTSL P+DP +WDWSW
Sbjct: 264 QHGLLMDGITWLLLPPDQSLAFLLADDGFDVWLVNTRGTKYSRGHTSLNPNDPAYWDWSW 323

Query: 142 DELVAFDLPATLQYVHNRTGQNMHYVGHSLGTLIALAAFSKHQLLDMLISAALISPIAHL 201
           DELVA+DLPAT QYVH++ GQ +HYVGHSLGTL+ALAAFSK QLL+M+ SAAL+SPIA++
Sbjct: 324 DELVAYDLPATFQYVHDQAGQKLHYVGHSLGTLMALAAFSKDQLLNMVRSAALLSPIAYV 383

Query: 202 GDVTSPIARNAADNFLGEVLFWLGVKEFDPRGKAAVQLLVEVCAKPGVDCINLLTSFTGQ 261
           G +TSP+ARNAADNF+ E L+WLG+ EF+PRG+A ++LL ++C KPGVDC NLL +FTGQ
Sbjct: 384 GQMTSPLARNAADNFMAEALYWLGINEFNPRGRAVIKLLKDICNKPGVDCTNLLNAFTGQ 443

Query: 262 NCCLNPSVSQNFLIHEPQPTATKNMIHLSQMIRSGTIAMYDYVDVIENIKHYGQSTPPTY 321
           NCCLN S+   FL HEPQ TATKNMIHL+QMIR G IAMYDY D  EN KHYGQ TPP Y
Sbjct: 444 NCCLNSSIVDVFLDHEPQSTATKNMIHLAQMIREGNIAMYDYNDEDENRKHYGQPTPPVY 503

Query: 322 NMTSIPNDFPLFLSYGGADALSDVNDVQLLLDNFKDHDGDKLVVQFREDYAHADFVMGEN 381
           NMTSIPND PLFLSYGGADALSDV DVQLLLD+ KDHDGDKLVVQ+R+DYAHAD+VMG+N
Sbjct: 504 NMTSIPNDLPLFLSYGGADALSDVKDVQLLLDSLKDHDGDKLVVQYRDDYAHADYVMGQN 563

Query: 382 AKQVVYDPLIAFFMLQ 396
           AKQVVYDPL+AFF LQ
Sbjct: 564 AKQVVYDPLVAFFRLQ 578

BLAST of Carg13625 vs. TrEMBL
Match: tr|A0A1U8P4V1|A0A1U8P4V1_GOSHI (Lipase OS=Gossypium hirsutum OX=3635 GN=LOC107955053 PE=3 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 8.1e-165
Identity = 279/412 (67.72%), Postives = 329/412 (79.85%), Query Frame = 0

Query: 1   MNSPDPFNPIVLLLLLCGFII--------------VSALEPGSVDGICKSLVEPHNYACE 60
           M   D    I+LL+L CG                   A+ P S +GICKS+VE H Y CE
Sbjct: 1   MEVADSVTCIILLILFCGSAAGNRKALYSFKAQENAIAMAPSSDNGICKSMVETHGYDCE 60

Query: 61  EHLVITNDGFILSMQRIP---SGGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVL 120
           EH V T DG+ILSMQRIP   SGG  +  N PPVLLQHG+LMD  TWLLLPPE SLAFVL
Sbjct: 61  EHTVTTQDGYILSMQRIPVGRSGG--TPGNRPPVLLQHGILMDGITWLLLPPEQSLAFVL 120

Query: 121 ADKGFDVWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMH 180
           +D G+DVW+AN+RGTK+S+GH SL P+DP +WDWSWDELVA+DLPAT QYV+++TGQ +H
Sbjct: 121 SDNGYDVWIANSRGTKYSKGHKSLSPNDPAYWDWSWDELVAYDLPATFQYVYDQTGQKLH 180

Query: 181 YVGHSLGTLIALAAFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLG 240
           YVGHS GTL+ALAA SK QLL+ML SAAL+ PIA++G +TSP+A+NAADNF+ E ++WLG
Sbjct: 181 YVGHSQGTLVALAALSKDQLLNMLRSAALLCPIAYMGQMTSPLAKNAADNFIAEAVYWLG 240

Query: 241 VKEFDPRGKAAVQLLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKN 300
           + EFDPRG A V LL ++C+KPG+DC NLLTSFTGQNCCLN S+   FL HEPQP+ATKN
Sbjct: 241 LSEFDPRGDAVVNLLKDICSKPGIDCTNLLTSFTGQNCCLNSSIVNIFLDHEPQPSATKN 300

Query: 301 MIHLSQMIRSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDV 360
           M+HL+QMIR GTI MYDY+D +EN+KHYGQ+TPP YNMTSIPNDFP+FLSYGG DALSDV
Sbjct: 301 MVHLAQMIRQGTIKMYDYIDEVENMKHYGQATPPAYNMTSIPNDFPVFLSYGGEDALSDV 360

Query: 361 NDVQLLLDNFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ 396
           NDV+LLL + KDHDGDKLVVQ+R DYAHAD+VM ENAKQ VYDPLIAFF LQ
Sbjct: 361 NDVKLLLGSLKDHDGDKLVVQYRYDYAHADYVMAENAKQDVYDPLIAFFRLQ 410

BLAST of Carg13625 vs. TrEMBL
Match: tr|A0A0D2Q2J2|A0A0D2Q2J2_GOSRA (Lipase OS=Gossypium raimondii OX=29730 GN=B456_001G217700 PE=3 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 8.1e-165
Identity = 279/412 (67.72%), Postives = 329/412 (79.85%), Query Frame = 0

Query: 1   MNSPDPFNPIVLLLLLCGFII--------------VSALEPGSVDGICKSLVEPHNYACE 60
           M   D    I+LL+L CG                   A+ P S +GICKS+VE H Y CE
Sbjct: 1   MEVADSVTCIILLILFCGSAAGNRKALYSFKAQENAIAMAPSSDNGICKSMVETHGYDCE 60

Query: 61  EHLVITNDGFILSMQRIP---SGGRTSSANGPPVLLQHGLLMDAATWLLLPPESSLAFVL 120
           EH V T DG+ILSMQRIP   SGG  +  N PPVLLQHG+LMD  TWLLLPPE SLAFVL
Sbjct: 61  EHTVTTQDGYILSMQRIPVGRSGG--TPGNRPPVLLQHGILMDGITWLLLPPEQSLAFVL 120

Query: 121 ADKGFDVWLANTRGTKFSQGHTSLGPDDPGFWDWSWDELVAFDLPATLQYVHNRTGQNMH 180
           +D G+DVW+AN+RGTK+S+GH SL P+DP +WDWSWDELVA+DLPAT QYV+++TGQ +H
Sbjct: 121 SDNGYDVWIANSRGTKYSKGHKSLSPNDPAYWDWSWDELVAYDLPATFQYVYDQTGQKLH 180

Query: 181 YVGHSLGTLIALAAFSKHQLLDMLISAALISPIAHLGDVTSPIARNAADNFLGEVLFWLG 240
           YVGHS GTL+ALAA SK QLL+ML SAAL+ PIA++G +TSP+A+NAADNF+ E ++WLG
Sbjct: 181 YVGHSQGTLVALAALSKDQLLNMLRSAALLCPIAYMGQMTSPLAKNAADNFIAEAVYWLG 240

Query: 241 VKEFDPRGKAAVQLLVEVCAKPGVDCINLLTSFTGQNCCLNPSVSQNFLIHEPQPTATKN 300
           + EFDPRG A V LL ++C+KPG+DC NLLTSFTGQNCCLN S+   FL HEPQP+ATKN
Sbjct: 241 LSEFDPRGDAVVNLLKDICSKPGIDCTNLLTSFTGQNCCLNSSIVNIFLDHEPQPSATKN 300

Query: 301 MIHLSQMIRSGTIAMYDYVDVIENIKHYGQSTPPTYNMTSIPNDFPLFLSYGGADALSDV 360
           M+HL+QMIR GTI MYDY+D +EN+KHYGQ+TPP YNMTSIPNDFP+FLSYGG DALSDV
Sbjct: 301 MVHLAQMIRQGTIKMYDYIDEVENMKHYGQATPPAYNMTSIPNDFPVFLSYGGEDALSDV 360

Query: 361 NDVQLLLDNFKDHDGDKLVVQFREDYAHADFVMGENAKQVVYDPLIAFFMLQ 396
           NDV+LLL + KDHDGDKLVVQ+R DYAHAD+VM ENAKQ VYDPLIAFF LQ
Sbjct: 361 NDVKLLLGSLKDHDGDKLVVQYRYDYAHADYVMAENAKQDVYDPLIAFFRLQ 410

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008456397.11.7e-20689.47PREDICTED: triacylglycerol lipase 2-like [Cucumis melo][more]
XP_004143194.18.4e-20688.97PREDICTED: triacylglycerol lipase 2-like [Cucumis sativus] >KGN47007.1 hypotheti... [more]
XP_022947370.15.7e-202100.00triacylglycerol lipase 2-like [Cucurbita moschata][more]
XP_022970785.16.9e-20099.71triacylglycerol lipase 2-like [Cucurbita maxima][more]
XP_023533928.11.7e-19899.13triacylglycerol lipase 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G14180.14.6e-11351.90Myzus persicae-induced lipase 1[more]
AT2G15230.16.2e-8641.13lipase 1[more]
AT1G73920.11.2e-1232.79alpha/beta-Hydrolases superfamily protein[more]
AT1G18460.11.0e-1131.15alpha/beta-Hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
sp|Q67ZU1|LIP2_ARATH8.2e-11251.90Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1[more]
sp|Q71DJ5|LIP1_ARATH1.1e-8441.13Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1[more]
sp|P80035|LIPG_CANLF8.4e-5636.59Gastric triacylglycerol lipase OS=Canis lupus familiaris OX=9615 GN=LIPF PE=1 SV... [more]
sp|Q29458|LIPG_BOVIN3.2e-5537.08Gastric triacylglycerol lipase OS=Bos taurus OX=9913 GN=LIPF PE=1 SV=1[more]
sp|P04634|LIPG_RAT3.2e-5536.80Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3C4E7|A0A1S3C4E7_CUCME1.1e-20689.47Lipase OS=Cucumis melo OX=3656 GN=LOC103496348 PE=3 SV=1[more]
tr|A0A0A0KF57|A0A0A0KF57_CUCSA5.6e-20688.97Lipase OS=Cucumis sativus OX=3659 GN=Csa_6G168240 PE=3 SV=1[more]
tr|A0A2N9ETQ2|A0A2N9ETQ2_FAGSY7.4e-16673.94Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS5826 PE=4 SV=1[more]
tr|A0A1U8P4V1|A0A1U8P4V1_GOSHI8.1e-16567.72Lipase OS=Gossypium hirsutum OX=3635 GN=LOC107955053 PE=3 SV=1[more]
tr|A0A0D2Q2J2|A0A0D2Q2J2_GOSRA8.1e-16567.72Lipase OS=Gossypium raimondii OX=29730 GN=B456_001G217700 PE=3 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0016788hydrolase activity, acting on ester bonds
Vocabulary: INTERPRO
TermDefinition
IPR006693AB_hydrolase_lipase
IPR029058AB_hydrolase
IPR025483Lipase_euk
IPR022742Hydrolase_4
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016788 hydrolase activity, acting on ester bonds

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13625-RACarg13625-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022742Serine aminopeptidase, S33PFAMPF12146Hydrolase_4coord: 99..355
e-value: 2.0E-6
score: 27.1
IPR025483Lipase, eukaryoticPIRSFPIRSF000862Steryl_ester_lipcoord: 11..395
e-value: 3.6E-119
score: 396.2
IPR029058Alpha/Beta hydrolase foldGENE3DG3DSA:3.40.50.1820coord: 30..393
e-value: 2.5E-110
score: 370.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILYSSF53474alpha/beta-Hydrolasescoord: 37..392
IPR006693Partial AB-hydrolase lipase domainPFAMPF04083Abhydro_lipasecoord: 37..92
e-value: 9.4E-19
score: 66.7
NoneNo IPR availablePANTHERPTHR11005:SF41SUBFAMILY NOT NAMEDcoord: 25..395
NoneNo IPR availablePANTHERPTHR11005LYSOSOMAL ACID LIPASE-RELATEDcoord: 25..395

The following gene(s) are paralogous to this gene:

None