Carg13536 (gene) Silver-seed gourd

NameCarg13536
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionIsocitrate lyase
LocationCucurbita_argyrosperma_scaffold_053 : 148636 .. 150466 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCATCCTTCTCTGTTCCATCAATGGTTCGTTGCAATTTCGATTCAAATTCCCAATGCAGGTTCTAACTAAGACTAACAATCGACATTTTTTGATGAACAGATAATGGAAGAAGAAGGGAGATTCGAAGCGGAAGTTGCAGAGGTACAGGCATGGTGGAATTCAGAGAGGTTCAAGCTAACACGACGCCCGTACTCTGCCAAAGACGTGGTGTCACTACGCGGCAGCTTAAGACAAAGCTACGCCTCGAACGAGTTGGCCAAGAAGCTATGGCGGACGCTCAAAACCCACCAAGCCAATGGCACGGCGTCGAGAACATTTGGCGCTCTAGACCCTGTTCAAGTGACGATGATGGCTAAGCATTTAGACACCATCTATGTCTCCGGCTGGCAATGCTCTTCCACCCATACCTCCACCAACGAACCAGGCCCGGACCTTGCTGATTACCCATATGACACCGTTCCGAACAAGGTCGAGCATCTGTTCTTCGCTCAACAGTATCACGACCGGAAACAGAGGGAGGCGAGGATGAGTATGAGCCGAGAGGAGCGAGCGAAAACCCCTTATGTTGACTATCTCAAACCCATCATCGCCGACGGGGACACCGGCTTCGGTGGCACCACCGCCACGGTGAAGCTCTGCAAGCTATTTGTGGAGCGCGGTGCCGCCGGTGTTCACATTGAGGATCAATCTTCGGTGACGAAAAAATGCGGGCATATGGCGGGAAAAGTGCTGGTGGCGGTTAGCGAGCATATCAATCGGCTCGTCGCAGCTCGATTACAGTTCGACGTGATGGGGGTCGAGACGGTTCTCGTGGCTCGAACCGATGCCGTCGGTGCCACTTTGATTCAAACGAACGTCGACAGTAGAGATCATCAGTTCATTTTGGGTGCAACGAATCCTAATTTGAGAGGCAAGAGCTTAGCTGGGGCATTGTCTGAAGCCATGGCGGCTGGAAAAACAGGGGCGGAACTTCAAGCGCTTGAAGATCAATGGATTTCCATGGCGCAATTGAAAACATTCTCAGAATGCGTGACCGACGCGATCATGAATATGAACGCAACAGAGAATGAAAAGAGAAGGAAGCTGGAGGAGTGGATGAGCCAATCAAGTTACGAGAAATGCCTATCGAACGAACAGGGGAGGAAAATCGTTGAGAATTTGGGGCTGAAGAATCTGTTCTGGGACTGGGATTTACCAAGAACCAGAGAAGGGTTTTACAGGTTCAAAGGGTCGGTAATGGCGGCGATTGTTCGTGGTTGGGCGTTCGCACCACATGCGGACCTAATCTGGATGGAAACATCAAGCCCCGACATGGTGGAGTGCACCAGTTTCGCAAAAGGGGTGAAATCGGCGCACCCAGAATTGATGTTGGCTTATAACCTCTCGCCATCTTTCAACTGGGATGCGTCGGGAATGAGCGACAAGGAGATGGAAGAGTTCATTCCAAGGATTGCGAGGCTTGGGTTCTGCTGGCAGTTCATCACACTCGCAGGTTTCCATGCGGATGCGTTGGTGATCGATACGTTTGCTAGAGATTATGCGAGGCGGGGAATGCTGGCTTATGTAGAGAGGATTCAAAGGGAAGAGAGAGCCAATGGAGTTGATACACTGGCTCATCAGAAATGGTCTGGAGCTAACTACTACGATCGGTATCTGAAGACAGTTCAGGGCGGCATTTCTTCTACCGCCGCCATGGGACGAGGCAAGGGACTAAATTCTAACTTTCTCCGACTCATTGAGGAACTGATTGATGTATGTTTTTTGAGTTTACAGGGGCGACGGAGGAGCAGTTCAAGGAGTCATGGACGAGGGAAGGAGCGGTGA

mRNA sequence

ATGGCTGCATCCTTCTCTGTTCCATCAATGATAATGGAAGAAGAAGGGAGATTCGAAGCGGAAGTTGCAGAGGTACAGGCATGGTGGAATTCAGAGAGGTTCAAGCTAACACGACGCCCGTACTCTGCCAAAGACGTGGTGTCACTACGCGGCAGCTTAAGACAAAGCTACGCCTCGAACGAGTTGGCCAAGAAGCTATGGCGGACGCTCAAAACCCACCAAGCCAATGGCACGGCGTCGAGAACATTTGGCGCTCTAGACCCTGTTCAAGTGACGATGATGGCTAAGCATTTAGACACCATCTATGTCTCCGGCTGGCAATGCTCTTCCACCCATACCTCCACCAACGAACCAGGCCCGGACCTTGCTGATTACCCATATGACACCGTTCCGAACAAGGTCGAGCATCTGTTCTTCGCTCAACAGTATCACGACCGGAAACAGAGGGAGGCGAGGATGAGTATGAGCCGAGAGGAGCGAGCGAAAACCCCTTATGTTGACTATCTCAAACCCATCATCGCCGACGGGGACACCGGCTTCGGTGGCACCACCGCCACGGTGAAGCTCTGCAAGCTATTTGTGGAGCGCGGTGCCGCCGGTGTTCACATTGAGGATCAATCTTCGGTGACGAAAAAATGCGGGCATATGGCGGGAAAAGTGCTGGTGGCGGTTAGCGAGCATATCAATCGGCTCGTCGCAGCTCGATTACAGTTCGACGTGATGGGGGTCGAGACGGTTCTCGTGGCTCGAACCGATGCCGTCGGTGCCACTTTGATTCAAACGAACGTCGACAGTAGAGATCATCAGTTCATTTTGGGTGCAACGAATCCTAATTTGAGAGGCAAGAGCTTAGCTGGGGCATTGTCTGAAGCCATGGCGGCTGGAAAAACAGGGGCGGAACTTCAAGCGCTTGAAGATCAATGGATTTCCATGGCGCAATTGAAAACATTCTCAGAATGCGTGACCGACGCGATCATGAATATGAACGCAACAGAGAATGAAAAGAGAAGGAAGCTGGAGGAGTGGATGAGCCAATCAAGTTACGAGAAATGCCTATCGAACGAACAGGGGAGGAAAATCGTTGAGAATTTGGGGCTGAAGAATCTGTTCTGGGACTGGGATTTACCAAGAACCAGAGAAGGGTTTTACAGGTTCAAAGGGTCGGTAATGGCGGCGATTGTTCGTGGTTGGGCGTTCGCACCACATGCGGACCTAATCTGGATGGAAACATCAAGCCCCGACATGGTGGAGTGCACCAGTTTCGCAAAAGGGGTGAAATCGGCGCACCCAGAATTGATGTTGGCTTATAACCTCTCGCCATCTTTCAACTGGGATGCGTCGGGAATGAGCGACAAGGAGATGGAAGAGTTCATTCCAAGGATTGCGAGGCTTGGGTTCTGCTGGCAGTTCATCACACTCGCAGGTTTCCATGCGGATGCGTTGGTGATCGATACGTTTGCTAGAGATTATGCGAGGCGGGGAATGCTGGCTTATGTAGAGAGGATTCAAAGGGAAGAGAGAGCCAATGGAGTTGATACACTGGCTCATCAGAAATGGTCTGGAGCTAACTACTACGATCGGTATCTGAAGACAGTTCAGGGCGGCATTTCTTCTACCGCCGCCATGGGACGAGGCAAGGGACTAAATTCTAACTTTCTCCGACTCATTGAGGAACTGATTGATGTATGTTTTTTGAGTTTACAGGGGCGACGGAGGAGCAGTTCAAGGAGTCATGGACGAGGGAAGGAGCGGTGA

Coding sequence (CDS)

ATGGCTGCATCCTTCTCTGTTCCATCAATGATAATGGAAGAAGAAGGGAGATTCGAAGCGGAAGTTGCAGAGGTACAGGCATGGTGGAATTCAGAGAGGTTCAAGCTAACACGACGCCCGTACTCTGCCAAAGACGTGGTGTCACTACGCGGCAGCTTAAGACAAAGCTACGCCTCGAACGAGTTGGCCAAGAAGCTATGGCGGACGCTCAAAACCCACCAAGCCAATGGCACGGCGTCGAGAACATTTGGCGCTCTAGACCCTGTTCAAGTGACGATGATGGCTAAGCATTTAGACACCATCTATGTCTCCGGCTGGCAATGCTCTTCCACCCATACCTCCACCAACGAACCAGGCCCGGACCTTGCTGATTACCCATATGACACCGTTCCGAACAAGGTCGAGCATCTGTTCTTCGCTCAACAGTATCACGACCGGAAACAGAGGGAGGCGAGGATGAGTATGAGCCGAGAGGAGCGAGCGAAAACCCCTTATGTTGACTATCTCAAACCCATCATCGCCGACGGGGACACCGGCTTCGGTGGCACCACCGCCACGGTGAAGCTCTGCAAGCTATTTGTGGAGCGCGGTGCCGCCGGTGTTCACATTGAGGATCAATCTTCGGTGACGAAAAAATGCGGGCATATGGCGGGAAAAGTGCTGGTGGCGGTTAGCGAGCATATCAATCGGCTCGTCGCAGCTCGATTACAGTTCGACGTGATGGGGGTCGAGACGGTTCTCGTGGCTCGAACCGATGCCGTCGGTGCCACTTTGATTCAAACGAACGTCGACAGTAGAGATCATCAGTTCATTTTGGGTGCAACGAATCCTAATTTGAGAGGCAAGAGCTTAGCTGGGGCATTGTCTGAAGCCATGGCGGCTGGAAAAACAGGGGCGGAACTTCAAGCGCTTGAAGATCAATGGATTTCCATGGCGCAATTGAAAACATTCTCAGAATGCGTGACCGACGCGATCATGAATATGAACGCAACAGAGAATGAAAAGAGAAGGAAGCTGGAGGAGTGGATGAGCCAATCAAGTTACGAGAAATGCCTATCGAACGAACAGGGGAGGAAAATCGTTGAGAATTTGGGGCTGAAGAATCTGTTCTGGGACTGGGATTTACCAAGAACCAGAGAAGGGTTTTACAGGTTCAAAGGGTCGGTAATGGCGGCGATTGTTCGTGGTTGGGCGTTCGCACCACATGCGGACCTAATCTGGATGGAAACATCAAGCCCCGACATGGTGGAGTGCACCAGTTTCGCAAAAGGGGTGAAATCGGCGCACCCAGAATTGATGTTGGCTTATAACCTCTCGCCATCTTTCAACTGGGATGCGTCGGGAATGAGCGACAAGGAGATGGAAGAGTTCATTCCAAGGATTGCGAGGCTTGGGTTCTGCTGGCAGTTCATCACACTCGCAGGTTTCCATGCGGATGCGTTGGTGATCGATACGTTTGCTAGAGATTATGCGAGGCGGGGAATGCTGGCTTATGTAGAGAGGATTCAAAGGGAAGAGAGAGCCAATGGAGTTGATACACTGGCTCATCAGAAATGGTCTGGAGCTAACTACTACGATCGGTATCTGAAGACAGTTCAGGGCGGCATTTCTTCTACCGCCGCCATGGGACGAGGCAAGGGACTAAATTCTAACTTTCTCCGACTCATTGAGGAACTGATTGATGTATGTTTTTTGAGTTTACAGGGGCGACGGAGGAGCAGTTCAAGGAGTCATGGACGAGGGAAGGAGCGGTGA

Protein sequence

MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASNELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGFGGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAELQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKIVENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTSFAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGRGKGLNSNFLRLIEELIDVCFLSLQGRRRSSSRSHGRGKER
BLAST of Carg13536 vs. NCBI nr
Match: XP_022947977.1 (isocitrate lyase-like [Cucurbita moschata])

HSP 1 Score: 1096.3 bits (2834), Expect = 0.0e+00
Identity = 543/545 (99.63%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN
Sbjct: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP
Sbjct: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE
Sbjct: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKL+EWMSQSSYEKCLSNEQGRKI
Sbjct: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLDEWMSQSSYEKCLSNEQGRKI 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
           VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS
Sbjct: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA
Sbjct: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LVIDTFAR+YARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA
Sbjct: 481 LVIDTFAREYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMGRG
Sbjct: 541 AMGRG 545

BLAST of Carg13536 vs. NCBI nr
Match: XP_023534113.1 (isocitrate lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1090.5 bits (2819), Expect = 0.0e+00
Identity = 540/545 (99.08%), Postives = 542/545 (99.45%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN
Sbjct: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP
Sbjct: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE
Sbjct: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKL+EWMS SSYEKCLSNEQGR+I
Sbjct: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLDEWMSHSSYEKCLSNEQGREI 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
           VE LGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS
Sbjct: 361 VEKLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FAKGVKS HPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA
Sbjct: 421 FAKGVKSVHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA
Sbjct: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMGRG
Sbjct: 541 AMGRG 545

BLAST of Carg13536 vs. NCBI nr
Match: XP_022970972.1 (isocitrate lyase-like [Cucurbita maxima])

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 539/545 (98.90%), Postives = 541/545 (99.27%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAASFSVPSMIMEEEGRFEAEVAEV AWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN
Sbjct: 1   MAASFSVPSMIMEEEGRFEAEVAEVHAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP
Sbjct: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE
Sbjct: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKL+EWMSQSSYEKCLSNEQGR+I
Sbjct: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLDEWMSQSSYEKCLSNEQGREI 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
           VE LGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS
Sbjct: 361 VEKLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FAKGVKS HPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA
Sbjct: 421 FAKGVKSVHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LVIDTFARDYARRGML YVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA
Sbjct: 481 LVIDTFARDYARRGMLGYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMGRG
Sbjct: 541 AMGRG 545

BLAST of Carg13536 vs. NCBI nr
Match: P49296.1 (RecName: Full=Isocitrate lyase; Short=ICL; AltName: Full=Isocitrase; AltName: Full=Isocitratsysase >CAA84632.1 isocitrate lyase [Cucumis sativus])

HSP 1 Score: 1065.8 bits (2755), Expect = 5.1e-308
Identity = 521/545 (95.60%), Postives = 536/545 (98.35%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPY+AKDVVSLRGSLRQSYASN
Sbjct: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           +LAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP
Sbjct: 61  DLAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPY+DYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MGVET+LVARTDAV ATLIQTNVD RDHQFILGATNPNLRGKSLAGAL+EAMAAGKTGAE
Sbjct: 241 MGVETILVARTDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGALAEAMAAGKTGAE 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQALEDQWISMAQLKTFSECVTDAIMN NATENEKRRKL+EWM+ SSYEKC+SNEQGR+I
Sbjct: 301 LQALEDQWISMAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREI 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
            E LGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPD+VECT+
Sbjct: 361 AEKLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTT 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FAKG+KS HPE MLAYNLSPSFNWDASGMSDK+MEEFIPRIARLGFCWQFITLAGFHADA
Sbjct: 421 FAKGMKSIHPETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LV+DTFARDYARRGMLAYVERIQREER NGVDTLAHQKWSGANYYDRYLKTVQGGISSTA
Sbjct: 481 LVVDTFARDYARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMG+G
Sbjct: 541 AMGKG 545

BLAST of Carg13536 vs. NCBI nr
Match: XP_004151880.1 (PREDICTED: isocitrate lyase [Cucumis sativus] >KGN63267.1 hypothetical protein Csa_2G420990 [Cucumis sativus])

HSP 1 Score: 1064.3 bits (2751), Expect = 1.5e-307
Identity = 520/545 (95.41%), Postives = 535/545 (98.17%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPY+AKDVVSLRGSLRQSYASN
Sbjct: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           +LAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP
Sbjct: 61  DLAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPY+DYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLV VSEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVGVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MGVET+LVARTDAV ATLIQTNVD RDHQFILGATNPNLRGKSLAGAL+EAMAAGKTGAE
Sbjct: 241 MGVETILVARTDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGALAEAMAAGKTGAE 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQALEDQWISMAQLKTFSECVTDAIMN NATENEKRRKL+EWM+ SSYEKC+SNEQGR+I
Sbjct: 301 LQALEDQWISMAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREI 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
            E LGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPD+VECT+
Sbjct: 361 AEKLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTT 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FAKG+KS HPE MLAYNLSPSFNWDASGMSDK+MEEFIPRIARLGFCWQFITLAGFHADA
Sbjct: 421 FAKGMKSIHPETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LV+DTFARDYARRGMLAYVERIQREER NGVDTLAHQKWSGANYYDRYLKTVQGGISSTA
Sbjct: 481 LVVDTFARDYARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMG+G
Sbjct: 541 AMGKG 545

BLAST of Carg13536 vs. TAIR10
Match: AT3G21720.1 (isocitrate lyase)

HSP 1 Score: 955.7 bits (2469), Expect = 1.3e-278
Identity = 460/545 (84.40%), Postives = 501/545 (91.93%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAASFSVPSMIMEEEGRFEAEVAEVQ WW+SERFKLTRRPY+A+DVV+LRG L+Q YASN
Sbjct: 1   MAASFSVPSMIMEEEGRFEAEVAEVQTWWSSERFKLTRRPYTARDVVALRGHLKQGYASN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           E+AKKLWRTLK+HQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP
Sbjct: 61  EMAKKLWRTLKSHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREER KTP+VDYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERTKTPFVDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MG ETVLVARTDAV ATLIQ+N+D+RDHQFILGATNP+LRGKSL+  L+E M  GK G  
Sbjct: 241 MGTETVLVARTDAVAATLIQSNIDARDHQFILGATNPSLRGKSLSSLLAEGMTVGKNGPA 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQ++EDQW+  A L TFSE V  AI  MN  ENEK ++L EW++ + YE CLSNEQGR +
Sbjct: 301 LQSIEDQWLGSAGLMTFSEAVVQAIKRMNLNENEKNQRLSEWLTHARYENCLSNEQGRVL 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
              LG+ +LFWDWDLPRTREGFYRF+GSV AA+VRGWAFA  AD+IWMET+SPD+ ECT 
Sbjct: 361 AAKLGVTDLFWDWDLPRTREGFYRFQGSVAAAVVRGWAFAQIADIIWMETASPDLNECTQ 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FA+G+KS  PE+MLAYNLSPSFNWDASGM+D++M EFIPRIARLG+CWQFITLAGFHADA
Sbjct: 421 FAEGIKSKTPEVMLAYNLSPSFNWDASGMTDQQMVEFIPRIARLGYCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LV+DTFA+DYARRGMLAYVERIQREER +GVDTLAHQKWSGANYYDRYLKTVQGGISSTA
Sbjct: 481 LVVDTFAKDYARRGMLAYVERIQREERTHGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMG+G
Sbjct: 541 AMGKG 545

BLAST of Carg13536 vs. TAIR10
Match: AT1G21440.1 (Phosphoenolpyruvate carboxylase family protein)

HSP 1 Score: 62.0 bits (149), Expect = 1.4e-09
Identity = 36/84 (42.86%), Postives = 49/84 (58.33%), Query Frame = 0

Query: 171 PIIADGDTGFGGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINR 230
           PIIAD DTG G      +  K  +  GAAG  +EDQ +  K+CGHM GK ++   EH  +
Sbjct: 115 PIIADADTGGGNALNVQRTVKDLIAAGAAGCFLEDQ-AWPKRCGHMRGKEVIPAEEHAAK 174

Query: 231 LVAARLQFDVMG-VETVLVARTDA 254
           + +AR   D +G  +  L+ARTDA
Sbjct: 175 IASAR---DAIGDADFFLIARTDA 194

BLAST of Carg13536 vs. TAIR10
Match: AT1G77060.1 (Phosphoenolpyruvate carboxylase family protein)

HSP 1 Score: 60.8 bits (146), Expect = 3.1e-09
Identity = 40/95 (42.11%), Postives = 55/95 (57.89%), Query Frame = 0

Query: 171 PIIADGDTGFGGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINR 230
           PIIAD DTG G      +  K  +  GAAG  +EDQ +  KKCGHM GK ++   EH  +
Sbjct: 117 PIIADADTGGGNALNIQRTVKDLIAAGAAGCFLEDQ-AWPKKCGHMRGKQVIPAEEHAAK 176

Query: 231 LVAARLQFDVMG-VETVLVARTDAVGATLIQTNVD 265
           + +AR   D +G  +  LVARTD V AT  ++ ++
Sbjct: 177 IASAR---DAIGDSDFFLVARTD-VRATSAKSGLE 206

BLAST of Carg13536 vs. Swiss-Prot
Match: sp|P49296|ACEA_CUCSA (Isocitrate lyase OS=Cucumis sativus OX=3659 PE=3 SV=1)

HSP 1 Score: 1065.8 bits (2755), Expect = 1.7e-310
Identity = 521/545 (95.60%), Postives = 536/545 (98.35%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPY+AKDVVSLRGSLRQSYASN
Sbjct: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           +LAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP
Sbjct: 61  DLAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPY+DYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MGVET+LVARTDAV ATLIQTNVD RDHQFILGATNPNLRGKSLAGAL+EAMAAGKTGAE
Sbjct: 241 MGVETILVARTDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGALAEAMAAGKTGAE 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQALEDQWISMAQLKTFSECVTDAIMN NATENEKRRKL+EWM+ SSYEKC+SNEQGR+I
Sbjct: 301 LQALEDQWISMAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREI 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
            E LGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPD+VECT+
Sbjct: 361 AEKLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTT 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FAKG+KS HPE MLAYNLSPSFNWDASGMSDK+MEEFIPRIARLGFCWQFITLAGFHADA
Sbjct: 421 FAKGMKSIHPETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LV+DTFARDYARRGMLAYVERIQREER NGVDTLAHQKWSGANYYDRYLKTVQGGISSTA
Sbjct: 481 LVVDTFARDYARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMG+G
Sbjct: 541 AMGKG 545

BLAST of Carg13536 vs. Swiss-Prot
Match: sp|P93110|ACEA_CUCMA (Isocitrate lyase OS=Cucurbita maxima OX=3661 PE=2 SV=1)

HSP 1 Score: 1058.5 bits (2736), Expect = 2.7e-308
Identity = 519/545 (95.23%), Postives = 533/545 (97.80%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MA SFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPY+AKDVVSLRGSLRQSYASN
Sbjct: 1   MATSFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           +LAKKLWRTLKTHQAN TASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHTSTNEPGP
Sbjct: 61  DLAKKLWRTLKTHQANSTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTSTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MGVETVLVARTDAV ATLIQTNVDSRDHQFILGATNPNLRGKSLAG L+EAMAAGKTGAE
Sbjct: 241 MGVETVLVARTDAVAATLIQTNVDSRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAE 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQALEDQWISMAQLKTFSECVTDAIMN N TE+EKRRKL+EWM+ SSYEKC+SNEQGR+I
Sbjct: 301 LQALEDQWISMAQLKTFSECVTDAIMNSNGTESEKRRKLDEWMNHSSYEKCISNEQGREI 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
            E LGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPD+VECT+
Sbjct: 361 AEKLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTT 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FAKGVKS HPE+MLAYNLSPSFNWDASGMSDK+MEEFIP IARLGFCWQFITLAGFHADA
Sbjct: 421 FAKGVKSVHPEIMLAYNLSPSFNWDASGMSDKQMEEFIPTIARLGFCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LVIDTFARDYARRGMLAYVERIQREER NGVDTLAHQKWSGANYYDRYLKTVQGGISSTA
Sbjct: 481 LVIDTFARDYARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMG+G
Sbjct: 541 AMGKG 545

BLAST of Carg13536 vs. Swiss-Prot
Match: sp|P17069|ACEA_GOSHI (Isocitrate lyase OS=Gossypium hirsutum OX=3635 PE=2 SV=1)

HSP 1 Score: 1010.4 bits (2611), Expect = 8.3e-294
Identity = 488/545 (89.54%), Postives = 521/545 (95.60%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAASFSVPSMIMEEEGRFE EVAEVQAWWNSERFKLTRRPYSA+DVV+LRGSL+QSY SN
Sbjct: 1   MAASFSVPSMIMEEEGRFETEVAEVQAWWNSERFKLTRRPYSARDVVALRGSLKQSYGSN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           E+AKKLW TLKTHQANGTASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHT+TNEPGP
Sbjct: 61  EMAKKLWTTLKTHQANGTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTTTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERA+TPYVDYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MGVETVLVARTDAV ATLIQTNVD+RDHQFILGATNPNLRGKSLA  L+E MAAGK G +
Sbjct: 241 MGVETVLVARTDAVAATLIQTNVDTRDHQFILGATNPNLRGKSLANMLAEGMAAGKNGPQ 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQA+ED W+++AQLKTFSECV DAI +MN TE+EKRR++ EWM+ SSY+KCLSNEQ R+I
Sbjct: 301 LQAIEDNWLAIAQLKTFSECVMDAIKSMNITEDEKRRRMNEWMNHSSYDKCLSNEQAREI 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
            E LGL+NLFWDWDLPRTREGFYRF+GSVMAAIVRGWAFAPHADLIWMETSSPDMVECT 
Sbjct: 361 AERLGLQNLFWDWDLPRTREGFYRFRGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTR 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FA+GVKS HPE+MLAYNLSPSFNWDASGM+D+ M +FIPRIA+LGFCWQFITLAGFHADA
Sbjct: 421 FAEGVKSMHPEIMLAYNLSPSFNWDASGMTDEHMRDFIPRIAKLGFCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LV DTFARD+ARRGMLAYVE+IQREER NGVDTLAHQKWSGAN+YDRYLKTVQGGISSTA
Sbjct: 481 LVTDTFARDFARRGMLAYVEKIQREERNNGVDTLAHQKWSGANFYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMG+G
Sbjct: 541 AMGKG 545

BLAST of Carg13536 vs. Swiss-Prot
Match: sp|P15479|ACEA_RICCO (Isocitrate lyase OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 4.7e-289
Identity = 480/545 (88.07%), Postives = 516/545 (94.68%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAASFS PSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPY+A+DVV+LRG+L+QSYASN
Sbjct: 1   MAASFSGPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTARDVVALRGNLKQSYASN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHT+TNEPGP
Sbjct: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTTTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERA+TPYVDYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MGVET+LVARTDA  A LIQ+NVD+RDHQFILG TNPNLRGKSLA  L+  MA GKTGAE
Sbjct: 241 MGVETLLVARTDAEAANLIQSNVDTRDHQFILGVTNPNLRGKSLATLLATGMANGKTGAE 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQA ED W++MAQLKTF ECV DAI NMNA E+EKRR++ EWM+ +SY+KCLS EQGR+I
Sbjct: 301 LQATEDNWLAMAQLKTFPECVMDAIKNMNAGEDEKRRRMNEWMNHTSYDKCLSYEQGREI 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
            + +GLKNLFWDWDLPRTREGFYRFKGSVMAA+VRG AFAPHAD+IWMET+ PD  ECT+
Sbjct: 361 ADRMGLKNLFWDWDLPRTREGFYRFKGSVMAAVVRGRAFAPHADIIWMETAKPDFAECTA 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FA+GVKS HPE+MLAYNLSPSFNWDASGM+D++M +FIPRIARLGFCWQFITL GFHADA
Sbjct: 421 FAEGVKSMHPEIMLAYNLSPSFNWDASGMTDEQMRDFIPRIARLGFCWQFITLGGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LVIDTFA+DYARRGMLAYVERIQREER NGVDTLAHQKWSGANYYDRYLKTVQGGISSTA
Sbjct: 481 LVIDTFAKDYARRGMLAYVERIQREERKNGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMG+G
Sbjct: 541 AMGKG 545

BLAST of Carg13536 vs. Swiss-Prot
Match: sp|P49297|ACEA_SOLLC (Isocitrate lyase OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 1.3e-283
Identity = 469/545 (86.06%), Postives = 513/545 (94.13%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAAS+SVPSMIMEEE RFEAEVAEVQAWWN+ERF+LT+R YSA+DVV+LRG++RQSYASN
Sbjct: 1   MAASYSVPSMIMEEERRFEAEVAEVQAWWNTERFRLTKRAYSARDVVALRGTMRQSYASN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           ELA+KLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP
Sbjct: 61  ELAQKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSM REERA+TP++DYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMCREERARTPFIDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GG TATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV
Sbjct: 181 GGATATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MG ETVLVARTDAV ATLIQTNVD+RDHQFILGA+NPNL+GK LA  LSEAMAAGKTG E
Sbjct: 241 MGTETVLVARTDAVAATLIQTNVDTRDHQFILGASNPNLKGKGLATHLSEAMAAGKTGPE 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQA+ED W+ MA+LKTFS+CVTDAI  MN  E EK+RKL EWM+ SSYEKCLS+EQ R++
Sbjct: 301 LQAIEDNWLGMAELKTFSQCVTDAIKKMNLAEYEKQRKLNEWMNHSSYEKCLSHEQAREV 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
            E LGL NLFWDWDLPRTREGFYRF+GSV AAIVRGWAFA + DL+WMETSSPDMVECT 
Sbjct: 361 AERLGLPNLFWDWDLPRTREGFYRFQGSVEAAIVRGWAFAEYCDLVWMETSSPDMVECTK 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           F++GVK+  PELMLAYNLSPSFNWDASGM+D +M +FIPRIA+LG+CWQFITLAGFHADA
Sbjct: 421 FSQGVKTLRPELMLAYNLSPSFNWDASGMNDNQMMDFIPRIAKLGYCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           L++DTFA+D+ARRGMLAYVE+IQREER+NGVDTLAHQKWSGANYYDR L+TVQGGI+STA
Sbjct: 481 LIVDTFAKDFARRGMLAYVEKIQREERSNGVDTLAHQKWSGANYYDRVLRTVQGGITSTA 540

Query: 541 AMGRG 546
           AMG+G
Sbjct: 541 AMGKG 545

BLAST of Carg13536 vs. TrEMBL
Match: tr|A0A0A0LR30|A0A0A0LR30_CUCSA (Isocitrate lyase OS=Cucumis sativus OX=3659 GN=Csa_2G420990 PE=3 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 9.8e-308
Identity = 520/545 (95.41%), Postives = 535/545 (98.17%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPY+AKDVVSLRGSLRQSYASN
Sbjct: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           +LAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP
Sbjct: 61  DLAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPY+DYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLV VSEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVGVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MGVET+LVARTDAV ATLIQTNVD RDHQFILGATNPNLRGKSLAGAL+EAMAAGKTGAE
Sbjct: 241 MGVETILVARTDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGALAEAMAAGKTGAE 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQALEDQWISMAQLKTFSECVTDAIMN NATENEKRRKL+EWM+ SSYEKC+SNEQGR+I
Sbjct: 301 LQALEDQWISMAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREI 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
            E LGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPD+VECT+
Sbjct: 361 AEKLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTT 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FAKG+KS HPE MLAYNLSPSFNWDASGMSDK+MEEFIPRIARLGFCWQFITLAGFHADA
Sbjct: 421 FAKGMKSIHPETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LV+DTFARDYARRGMLAYVERIQREER NGVDTLAHQKWSGANYYDRYLKTVQGGISSTA
Sbjct: 481 LVVDTFARDYARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMG+G
Sbjct: 541 AMGKG 545

BLAST of Carg13536 vs. TrEMBL
Match: tr|A0A1S3C1U5|A0A1S3C1U5_CUCME (Isocitrate lyase OS=Cucumis melo OX=3656 GN=LOC103495946 PE=3 SV=1)

HSP 1 Score: 1061.2 bits (2743), Expect = 8.3e-307
Identity = 520/545 (95.41%), Postives = 534/545 (97.98%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPY+AKDVVSLRGSLRQSYASN
Sbjct: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           +LAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP
Sbjct: 61  DLAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPY+DYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MGVET+LVARTDAV ATLIQTNVD RDHQFILGATNPNLRGKSLAG L+EAMAAGKTGAE
Sbjct: 241 MGVETILVARTDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAE 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQALEDQWISMAQLKTFSECVTDAIMN  ATE+EKRRKL+EWM+ SSYEKC+SNEQGR+I
Sbjct: 301 LQALEDQWISMAQLKTFSECVTDAIMNTIATESEKRRKLDEWMNHSSYEKCISNEQGREI 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
            E LGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPD+VECT+
Sbjct: 361 AEKLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTT 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FAKGVKS HPE MLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA
Sbjct: 421 FAKGVKSIHPETMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LV+DTFARDYARRGMLAYVERIQREER NGVDTLAHQKWSGANYYDRYLKTVQGGISSTA
Sbjct: 481 LVVDTFARDYARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMG+G
Sbjct: 541 AMGKG 545

BLAST of Carg13536 vs. TrEMBL
Match: tr|A0A1U8I7C5|A0A1U8I7C5_GOSHI (Isocitrate lyase OS=Gossypium hirsutum OX=3635 GN=LOC107893568 PE=3 SV=1)

HSP 1 Score: 1013.1 bits (2618), Expect = 2.6e-292
Identity = 489/545 (89.72%), Postives = 521/545 (95.60%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAASFSVPSMIMEEEGRFE EVAEVQAWWNSERFKLTRRPY A+DVV+LRGSL+QSY SN
Sbjct: 1   MAASFSVPSMIMEEEGRFETEVAEVQAWWNSERFKLTRRPYQARDVVALRGSLKQSYGSN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           E+AKKLW TLKTHQANGTASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHT+TNEPGP
Sbjct: 61  EMAKKLWTTLKTHQANGTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTTTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPN+VEHLFFAQQYHDRKQREARMSMSREERA+TPYVDYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNQVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MGVETVLVARTDAV ATLIQTNVD+RDHQFILGATNPNLRGKSLA  L+E MAAGK G +
Sbjct: 241 MGVETVLVARTDAVAATLIQTNVDTRDHQFILGATNPNLRGKSLANMLAEGMAAGKNGPQ 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQA+ED W++MAQLKTFSECV DAI NMN TE+EKRR++ EWM+ SSY+KCLSNEQ R+I
Sbjct: 301 LQAIEDNWLAMAQLKTFSECVMDAIKNMNITEDEKRRRMNEWMNHSSYDKCLSNEQAREI 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
            E LGLKNLFWDWDLPRTREGFYRF+GSVMAAIVRGWAFAPHADLIWMETSSPDMVECT 
Sbjct: 361 AERLGLKNLFWDWDLPRTREGFYRFRGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTR 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FA+GVKS HPE+MLAYNLSPSFNWDASGM+D++M +FIPRIA+LGFCWQFITLAGFHADA
Sbjct: 421 FAEGVKSMHPEIMLAYNLSPSFNWDASGMTDEQMRDFIPRIAKLGFCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LV DTFARD+ARRGMLAYVE+IQREER NGVDTLAHQKWSGAN+YDRYLKTVQGGISSTA
Sbjct: 481 LVTDTFARDFARRGMLAYVEKIQREERNNGVDTLAHQKWSGANFYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMG+G
Sbjct: 541 AMGKG 545

BLAST of Carg13536 vs. TrEMBL
Match: tr|A0A0D2VD74|A0A0D2VD74_GOSRA (Isocitrate lyase OS=Gossypium raimondii OX=29730 GN=B456_013G099800 PE=3 SV=1)

HSP 1 Score: 1012.7 bits (2617), Expect = 3.4e-292
Identity = 489/545 (89.72%), Postives = 522/545 (95.78%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAASFSVPSMIMEEEGRFE EVAEVQAWWNSERFKLTRRPYSA+DVV+LRGSL+QSY SN
Sbjct: 1   MAASFSVPSMIMEEEGRFETEVAEVQAWWNSERFKLTRRPYSARDVVALRGSLKQSYGSN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           E+AKKLW TLKTHQANGTASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHT+TNEPGP
Sbjct: 61  EMAKKLWTTLKTHQANGTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTTTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERA+TPYVDYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MGVETVLVARTDAV ATLIQTNVD+RDHQFILGATNPNLRGKSLA  L+E MAAGK G +
Sbjct: 241 MGVETVLVARTDAVAATLIQTNVDTRDHQFILGATNPNLRGKSLANMLAEGMAAGKNGPQ 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQA+ED W+++AQLKTFSECV DAI +MN TE+EKRR++ EWM+ SSY+KCLSNEQ R+I
Sbjct: 301 LQAIEDNWLAIAQLKTFSECVMDAIKSMNITEDEKRRRMNEWMNHSSYDKCLSNEQAREI 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
            E LGLKNLFWDWDLPRTREGFYRF+GSVMAAIVRGWAFAPHADLIWMETSSPDMVECT 
Sbjct: 361 AERLGLKNLFWDWDLPRTREGFYRFRGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTR 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FA+GVKS HPE+MLAYNLSPSFNWDASGM+D++M +FIPRIA+LGFCWQFITLAGFHADA
Sbjct: 421 FAEGVKSMHPEIMLAYNLSPSFNWDASGMTDEQMRDFIPRIAKLGFCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LV DTFARD+ARRGMLAYVE+IQREER NGVDTLAHQKWSGAN+YDRYLKTVQGGISSTA
Sbjct: 481 LVTDTFARDFARRGMLAYVEKIQREERNNGVDTLAHQKWSGANFYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMG+G
Sbjct: 541 AMGKG 545

BLAST of Carg13536 vs. TrEMBL
Match: tr|A0A1U8KSS6|A0A1U8KSS6_GOSHI (Isocitrate lyase OS=Gossypium hirsutum OX=3635 GN=LOC107920350 PE=3 SV=1)

HSP 1 Score: 1011.1 bits (2613), Expect = 9.8e-292
Identity = 488/545 (89.54%), Postives = 522/545 (95.78%), Query Frame = 0

Query: 1   MAASFSVPSMIMEEEGRFEAEVAEVQAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASN 60
           MAASFSVPSMIMEEEGRFE EVAEVQAWWNSERFKLTRRPYSA+DVV+LRGSL+QSY SN
Sbjct: 1   MAASFSVPSMIMEEEGRFETEVAEVQAWWNSERFKLTRRPYSARDVVALRGSLKQSYGSN 60

Query: 61  ELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGP 120
           E+AKKLW TLKTHQANGTASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHT+TNEPGP
Sbjct: 61  EMAKKLWTTLKTHQANGTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTTTNEPGP 120

Query: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGF 180
           DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERA+TPYVDYLKPIIADGDTGF
Sbjct: 121 DLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGF 180

Query: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240
           GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV
Sbjct: 181 GGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDV 240

Query: 241 MGVETVLVARTDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAE 300
           MGVETVLVARTDAV ATLIQTNVD+RDHQFILGATNPNLRGKSLA  L+E MAAGK G +
Sbjct: 241 MGVETVLVARTDAVAATLIQTNVDTRDHQFILGATNPNLRGKSLANMLAEGMAAGKNGPQ 300

Query: 301 LQALEDQWISMAQLKTFSECVTDAIMNMNATENEKRRKLEEWMSQSSYEKCLSNEQGRKI 360
           LQA+ED W+++AQLKTFSECV DAI +MN TE+EKRR++ EWM+ SSY+KCLSNEQ R+I
Sbjct: 301 LQAIEDNWLAIAQLKTFSECVMDAIKSMNITEDEKRRRMNEWMNHSSYDKCLSNEQAREI 360

Query: 361 VENLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTS 420
            E LGL+NLFWDWDLPRTREGFYRF+GSVMAAIVRGWAFAPHADLIWMETSSPDMVECT 
Sbjct: 361 AERLGLQNLFWDWDLPRTREGFYRFRGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTR 420

Query: 421 FAKGVKSAHPELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADA 480
           FA+GVKS HPE+MLAYNLSPSFNWDASGM+D++M +FIPRIA+LGFCWQFITLAGFHADA
Sbjct: 421 FAEGVKSMHPEIMLAYNLSPSFNWDASGMTDEQMRDFIPRIAKLGFCWQFITLAGFHADA 480

Query: 481 LVIDTFARDYARRGMLAYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTA 540
           LV DTFARD+ARRGMLAYVE+IQREER NGVDTLAHQKWSGAN+YDRYLKTVQGGISSTA
Sbjct: 481 LVTDTFARDFARRGMLAYVEKIQREERNNGVDTLAHQKWSGANFYDRYLKTVQGGISSTA 540

Query: 541 AMGRG 546
           AMG+G
Sbjct: 541 AMGKG 545

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022947977.10.0e+0099.63isocitrate lyase-like [Cucurbita moschata][more]
XP_023534113.10.0e+0099.08isocitrate lyase-like [Cucurbita pepo subsp. pepo][more]
XP_022970972.10.0e+0098.90isocitrate lyase-like [Cucurbita maxima][more]
P49296.15.1e-30895.60RecName: Full=Isocitrate lyase; Short=ICL; AltName: Full=Isocitrase; AltName: Fu... [more]
XP_004151880.11.5e-30795.41PREDICTED: isocitrate lyase [Cucumis sativus] >KGN63267.1 hypothetical protein C... [more]
Match NameE-valueIdentityDescription
AT3G21720.11.3e-27884.40isocitrate lyase[more]
AT1G21440.11.4e-0942.86Phosphoenolpyruvate carboxylase family protein[more]
AT1G77060.13.1e-0942.11Phosphoenolpyruvate carboxylase family protein[more]
Match NameE-valueIdentityDescription
sp|P49296|ACEA_CUCSA1.7e-31095.60Isocitrate lyase OS=Cucumis sativus OX=3659 PE=3 SV=1[more]
sp|P93110|ACEA_CUCMA2.7e-30895.23Isocitrate lyase OS=Cucurbita maxima OX=3661 PE=2 SV=1[more]
sp|P17069|ACEA_GOSHI8.3e-29489.54Isocitrate lyase OS=Gossypium hirsutum OX=3635 PE=2 SV=1[more]
sp|P15479|ACEA_RICCO4.7e-28988.07Isocitrate lyase OS=Ricinus communis OX=3988 PE=2 SV=1[more]
sp|P49297|ACEA_SOLLC1.3e-28386.06Isocitrate lyase OS=Solanum lycopersicum OX=4081 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A0A0LR30|A0A0A0LR30_CUCSA9.8e-30895.41Isocitrate lyase OS=Cucumis sativus OX=3659 GN=Csa_2G420990 PE=3 SV=1[more]
tr|A0A1S3C1U5|A0A1S3C1U5_CUCME8.3e-30795.41Isocitrate lyase OS=Cucumis melo OX=3656 GN=LOC103495946 PE=3 SV=1[more]
tr|A0A1U8I7C5|A0A1U8I7C5_GOSHI2.6e-29289.72Isocitrate lyase OS=Gossypium hirsutum OX=3635 GN=LOC107893568 PE=3 SV=1[more]
tr|A0A0D2VD74|A0A0D2VD74_GOSRA3.4e-29289.72Isocitrate lyase OS=Gossypium raimondii OX=29730 GN=B456_013G099800 PE=3 SV=1[more]
tr|A0A1U8KSS6|A0A1U8KSS6_GOSHI9.8e-29289.54Isocitrate lyase OS=Gossypium hirsutum OX=3635 GN=LOC107920350 PE=3 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO:0019752carboxylic acid metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0004451isocitrate lyase activity
Vocabulary: INTERPRO
TermDefinition
IPR015813Pyrv/PenolPyrv_Kinase-like_dom
IPR018523Isocitrate_lyase_ph_CS
IPR006254Isocitrate_lyase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006097 glyoxylate cycle
biological_process GO:0019752 carboxylic acid metabolic process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004451 isocitrate lyase activity
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13536-RACarg13536-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006254Isocitrate lyaseTIGRFAMTIGR01346TIGR01346coord: 22..545
e-value: 2.3E-260
score: 862.4
IPR006254Isocitrate lyasePFAMPF00463ICLcoord: 21..545
e-value: 2.3E-270
score: 897.4
IPR006254Isocitrate lyasePIRSFPIRSF001362ICLcoord: 12..333
e-value: 5.4E-63
score: 211.1
coord: 368..552
e-value: 8.9E-52
score: 174.1
IPR006254Isocitrate lyasePANTHERPTHR21631ISOCITRATE LYASE/MALATE SYNTHASEcoord: 1..546
NoneNo IPR availableGENE3DG3DSA:1.10.10.850coord: 262..377
e-value: 1.1E-231
score: 771.6
NoneNo IPR availableGENE3DG3DSA:3.20.20.60coord: 378..530
e-value: 1.1E-231
score: 771.6
coord: 18..261
e-value: 1.1E-231
score: 771.6
NoneNo IPR availablePANTHERPTHR21631:SF26ISOCITRATE LYASEcoord: 1..546
NoneNo IPR availableCDDcd00377ICL_PEPMcoord: 75..489
e-value: 7.71635E-45
score: 158.806
IPR018523Isocitrate lyase/phosphorylmutase, conserved sitePROSITEPS00161ISOCITRATE_LYASEcoord: 211..216
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILYSSF51621Phosphoenolpyruvate/pyruvate domaincoord: 10..535

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg13536Carg03142Silver-seed gourdcarcarB029
The following block(s) are covering this gene:
GeneOrganismBlock
Carg13536Wax gourdcarwgoB0031
Carg13536Cucurbita pepo (Zucchini)carcpeB0040
Carg13536Silver-seed gourdcarcarB034
Carg13536Silver-seed gourdcarcarB035
Carg13536Silver-seed gourdcarcarB036
Carg13536Silver-seed gourdcarcarB037
Carg13536Cucumber (Gy14) v2carcgybB0032
Carg13536Cucumber (Gy14) v1carcgyB0028
Carg13536Cucumber (Gy14) v1carcgyB0035
Carg13536Cucurbita maxima (Rimu)carcmaB0043
Carg13536Cucurbita maxima (Rimu)carcmaB0037
Carg13536Cucurbita maxima (Rimu)carcmaB0038
Carg13536Cucurbita moschata (Rifu)carcmoB0037
Carg13536Cucurbita moschata (Rifu)carcmoB0039
Carg13536Cucurbita moschata (Rifu)carcmoB0040
Carg13536Cucurbita moschata (Rifu)carcmoB0045
Carg13536Cucurbita moschata (Rifu)carcmoB0046
Carg13536Cucurbita pepo (Zucchini)carcpeB0041
Carg13536Cucurbita pepo (Zucchini)carcpeB0045
Carg13536Cucurbita pepo (Zucchini)carcpeB0046
Carg13536Cucurbita pepo (Zucchini)carcpeB0047
Carg13536Wild cucumber (PI 183967)carcpiB0030
Carg13536Wild cucumber (PI 183967)carcpiB0034
Carg13536Cucumber (Chinese Long) v2carcuB0032
Carg13536Cucumber (Chinese Long) v3carcucB0030
Carg13536Cucumber (Chinese Long) v3carcucB0034
Carg13536Bottle gourd (USVL1VR-Ls)carlsiB028
Carg13536Bottle gourd (USVL1VR-Ls)carlsiB031
Carg13536Melon (DHL92) v3.5.1carmeB0033
Carg13536Melon (DHL92) v3.6.1carmedB0035
Carg13536Melon (DHL92) v3.6.1carmedB0034
Carg13536Watermelon (Charleston Gray)carwcgB0028
Carg13536Watermelon (Charleston Gray)carwcgB0032
Carg13536Watermelon (Charleston Gray)carwcgB0035
Carg13536Watermelon (97103) v1carwmB0029
Carg13536Watermelon (97103) v1carwmB0031
Carg13536Watermelon (97103) v1carwmB0032
Carg13536Watermelon (97103) v2carwmbB0026
Carg13536Watermelon (97103) v2carwmbB0027