Carg13450 (gene) Silver-seed gourd

NameCarg13450
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionE3 ubiquitin-protein ligase makorin
LocationCucurbita_argyrosperma_scaffold_049 : 548945 .. 554739 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTCCGAACGAACAATGAAGTGCGTGGAATTCGTTCTTTTTTCCACTTTCCATTTCCCTCCACTGCTTATATCTTATTCAAATCTTCTCTTACTTGTTTAATCAACCTTTTCAATCGTCTTTTCTTCCATTTTCCGCTTCTAACTTCGATCGCCATGTCTAAAAGGTGAGGTTCTGTTCTTTTGTTTCGATTCTGCTTCTTTCTATGTTCTTGTTGATTTTGTCTGGCAGATGCTTGTTTGTTCGTTTTCTCGTGTATTTTTTAAAAGTTTTGATGATTGGATGGCAGATTACGCGCTTTGATTCTTTTTTTCTTTTTCTTGAATAGTATCTTCCGGTTCATGGTTTTCTTTGTTTGGATGTACCTTCTCGAGTAATCTTGATCATGGGTCTTTGGTATGTGGTTTGATTATTAGGTGTATGCTATTTTGAGGGGCTGGGTGATCAAATATGTGGTTGGAAATGTTTGGAATTTGAGTTTCACGAAAGCTGTTTTGTTGGGTGGTTGTTGTTGAGGCTGGACTTTGTGTTTATGAAGTTTTGTTATTTTATATCTTGGAATTAACACGTTCTTAGCGTCTTAATTTACTTTGGTGGATTGTTTAGGGTTCTATGCAAGTATTTTGCGCATGGAGCATGCTTGAAAGGGGAGTATTGTGAGTTTTCGCATGACTGGACGGCTCCACCTAGCAATGTAAATATTACTTCCACTCCTCTGCATTGCTATGCATTTTAATGTAAACCAACTGTCAGTTTGGCACTCCGATCATTCTTTCTTTCTGATGTATCATTCGTGAAACAGGTTTGCACTTACTATCAAAAGGGAAGGTGTGCTTTTGGCAGTCGATGCAGATATGACCATGTTAAAGTTTCTCATGCAGAGTCATCAGCTTCTTCTAATTCTCAGCAACCTCACGTGTCTGAATCTGTTTCAATAGCTCATACTCTATTATCAAGTCCACTTTCTCCTTCACCTGGGTCGACAATTGAGCTTGCTGGTTCAAAGATATCCTGTCTTCCCTCGCACGTTGAATCTGCTTGGAGTGTAGAGTGTGAGCTTCCATCTGGTCTTGATGATTGGGGGAGTAGGGAGACAAGGATTATTAGTCCAGCCGATTTACCTATTTGCTCTTTTTCTGCTGCTGGTACTAATTGTCCGCGTGGTGATAATTGCCCTCACATTCATGGGGACTTATGTCCCACGTGCGGAAAACAATGTTTACATCCTTTTAGGACGGATGAAAGGGAGGAGCATATGAAGGCATGTGAGAAAAAGCAAAAACATCTCGAGTCTTTGAAACATAGCCAAGAAATAGAGTGTAGTGTCTGCCTCGAGCGTGTTCTTTCTAAAACCGAAATAGCCGAGAGGAAGTTTGGGCTTCTATCTGAATGTGATCATCCGTTCTGCATTTCTTGTATTAGAAACTGGCGTAATAGTTCATCTACCTCCGGAATGGATGTTAACAGTGCTTTAAGAGCCTGCCCAATTTGTAGGAAGCTATCATACTTTGTTATTCCAAGCGTCATTTGGTATTCTTCCAAGGAAGAAAAACAAGAGATCGTTGATAGCTACAAGTCAAAGCTGCGGTAAATTTATGTTCTTAATTCCTAATATTTGATTGCATTCTGGATACTATTAACTTTTGTCCATTCTGTTCCCATTTTTTATGTTTTATAACCAAACTCATTTTTGTCTATGCCATATCAAATGTAATCTGGAAAGCTCCATAGTCCACCGAATGATCAATATGCTTTCTAGAAGAATGGAAACTGCATAACTGTTCTAAAGTAATTGTGTGAGCTGTTGAAATAATTTCTATACTCTGATCTTGCAACTTTATATTATTTTCATGCACAGATCAATCGATTGCAAGCACTTTAACTTTGGAAATGGGAACTGCCCATTTGGATCTAGCTGTTTTTACAAGGTAAATTTTTCTCTGAAATCATCTACTTCAGTGTTCTTATCCTGATTGTGCTCTTCTAAGCAAGCTGGATGTTGCTGTAGCCTTTGGAATTGCAATTTTCCTGAAATTTTTTCTTCTTCCTTTTTTTTTTTTCCCCCTTTATTCCTTTATCCATATTGCTTTTTAACCGATCATTGATATGCTGAATGGAAATCTCAACCTGTTTGGTTGTTCTAATATTTGAATGCCTATGAAGTATGTTGATTGGTTACATCTTTGATTTCATGGACATGAGTTTATGGAAGGAATTCTTTTTGTGATCTGGAGACGGAAATATAGTGCAACAGAAGATTCTTTTTGTTCTTATGATCCTCCAATGGTTTTATAAGCGTCTCCATTTACCATGGCCATCCAATCATGGTGGGTTAGATATGTGGAATCGTACAATTTGAACTTGCATGTTGACAAAGTAAATTTGCTAATCATTTGGCCAGCTTTTTCACTATGTTTTTGTGCAATTTTCTTGCTATTAGGTGCTCATAATAATAGACTACCAATATGATGAAAAATATATAAATTTCTGGTTCAGTACCTCTTAGTGTGTTTCATTTTGTTCTTTTGACACAATGGTAAGGGTGCTTCTTTCTTGTCCATTTTTTTTTAAACAAAACCAAAGGATGCCTCCATTTCTCATAGGTATCCTCTCTTGGGAAGCCCTGTTTCAGTTAGAATAGTTCGAGGGCGAGGATGAGGGGTGAAGTCCCTTTCTTTGCTCCCACCGGTGTTCCTACTATAACCAAATCTCATAAATAATAACTTGCTTTGGTGTATACTATAGATAATCTATATCCATTATCTTTTCGTTTTCTGTTTCTAAAATTCATGCTTTATAACCAATTTCACTGTTCTGTAATCTATTTTTAAAAATCCTAAACAAATTATTTTTAGAAATTGTGCTTGATGGGAACTAAGAATTTAAAAGTGCTTTTAAAATGTGATAAACAAAAAAATGTATGATCTTAAAATTTACTTGAGTCTTTGAATAACAATTTTCTTTAGTCTTCAATTTAGTTTTGAGGATATTTTAAAAGCCTACCTTTTTTTTTTAATTAAAAATAAAATGTAAAGAATATTTTTAATAGTTAAAAGAAAACCCTCCAAATTTTGGTTTTATGTATTTAAAATTTGGCTGAGAATTTTAAACTGGCTTTTGAAAAGCAGAAAACAAAAAAACTAAACTAAGAAACGGTTGGTTAATTTTCGAAAAATTAAAATTCTATGTTTATTAAGCAAGGCCTAAGTTTTATCTACAAATGTCCATGTAATTAGTGGGGATGATTTCACAATTTCTCAAACAAAATCTTTGCAATTACTTTTGTTGCCGTCTTAGAAAATTTTCTGGTCTCAATCTGGCTGAAAGTGATGTGCTTACTACTTTGGCTGATTGGCTCTGCAACCTCTGTTTAGTAATGTCTTATCAGTCTTTTAGCTAAATTACCAATATGATGAAATTATTTAATTTTGCAAACCAATATTTTATTGCTAGACCTCCTATAAAAAAAAGAAAAAGAATGTATTGCTAGACCTGTTATAATTGATTGGTCTGTTTTAAGGTGAAGATAATAGACCTCTCTGTTTCAAATTAATAAAATTAATATACTTATAGCTATTGGATTTAAAAGTGTTATCATGAGGATATTGTAGGTTAGCACCATAGAATGACTGGAGGGACCTCTTCTAAATTGCGTTCTTTTAATTAGGAAAACTGTTATTGGAATCATTCATCAGTTCTAAAGCCAATCCATATGCTTGCAAATCATTGGCGCGTGTCTATTTTGGAGAATAAATATGCAACTGCTCTGTCACATTCTTCTGTCTCCCAAAAACTTTCAGTCAGCTTTTGTGGTAAATATTGAAAGATGTGGTAGACATAAGCAGTTCTGTTCATTGCGCATTCTGTTGTCATTTAATTTCATTAGAGCAGATTGGCTTTCCAATCTTTTGATTGATACAATGTTTGTTGTGTTCCAGCACTTGGTCAAGCCTGGTTCATACACATGGAAATATCACAGGCCACCTCTGCTCCGACCACGATCACAGAGAACTCCTCCTAGCAGATCTAATATTGTGGATATGGATGCCTTTTTTGATATGTTTGAAGACTTAGAGGATTTGGATTTTAGTGCTGAAGATTTAGATTTTGAAGATTTAACACCTTTGGAGATGGCATTTGTTCTAATGCAGTTGGGTTGTGAAGCCAACTTGTCAAGTGATGAGGAGAATTATAGTTTTTGAACTACATTTTTTATAGGTAGTTGTGCCTGATGTAAATTGGGTCTTTCACGAGTTCCTTTCTTGTCTTTGCCCAGATATTTGCTATTCTAGCATTTTTGGCCACATAGCAGTGCCCTACGTGCAAAAATACAGGCTCGTAGACTTTGTTAATTGTCACAAACGCCATTATTGGGGAATGATACTTCACAATAAGCTTTAGTCATTTGTCCTAGCGCAAATTAGTTGCTGAGTTACCACGATGACGATGATCTATATATGGGAGACCCCTTTTTGTTATGTGTAGATTATACTATGACTTCGGAAGTGTTGTATGTTACCGCTGGGAATTAGGAATATCTGTTACTTGCTATCTTTCATCATTCATAGATTTTATGCGGAGTTTTGTTTCACGCTCTTGCTTTCCCAAGCCAATTGTGCTCTCCTCATGTTTGATTTGGTGATTTCTTTGTTTGCAGTTGTGAAGTGTTTTTTTTTCTTTTCCTTCGAATATTTGGATTCTTTACTTCCCAAATCTTATATGTCATTTTCTGCCTTTGAAGTGTTTGCAATTCTGCCTTTTAGTCTGCTGTATTAACTTCAAAATGAGATACAAATTTTGATCTGATTTTCTCATTGCAGCACGCACATCGGGATGGCCGGTTGGAAGAGGTAGCACTGCGTCACATTGGGAATCAAGACGGCCTCACTATTATAGCTAAAAACATTAGGTTTTGTTTTGTGCATTTGCTTGATATTGTCCATTTCTTGTGCAATTTCGCCCTTTATTAATTTGATGCTATTTCCAGGTTGTCGGACTTCCTTAGTGACTTGCATATCAGGTGAAAGAACCATATTATCAGGTATGAAAACTCACGAAGCAGCCCTCACCCCTAAGCTCAGCTTCATGCCATTTGCCGTCTACTGTATGATATTTTCACATTTTAATATGATAATCATTGAATCTTGAAAACTTATGTTGTTGCTAGTCATAAAGTCTTGTTTGGACTTTGAAATTCAGCATCTTTATCAATTGACACCTAATATATTTATAAGAAACGGAGTTTATTAATCTTGGTACACAACCTGAGATCACTCTATTTCAAACTCGTTCTTGTTCCATATGTAACACTCTTGTCCATGAAATGCCAAACTTTGTGCATTGCATTAGTCTCGAGTTAGGCTTGCTTAGGAAATCAGTTATTAACAATTTAGTCCATGAAATGCCAAACTTTGTGCATTTCATTAGGCTTGAGTTAGGCTTGTATTAACGATTTAGTCCATGAAATGCCGAACTCTGTACTCTGTGTATTGTTGTAAAGTCTTCTTTTACAGAAATCTAAGAGTAGTTTGAAAACGCGTTTCAGGTGTCGAATTGCTTGCTGGAGTCCGATAATGAGAAATGGAAGGTGGGTTATTGAACACGGTGATTCGAACTTCATCATCTTCTATGAATGGAAATAAAGCTAAAGACATTGTCATACTGAGAAATGAATGTAGTAAGTTGAAGCCTTATTCTTGGGATGGCAAAATAAAGGGTTTTTCACATCAGATGTAGCACAGCTGAATAGCTCAATAAATTGCGTCGTTGGTTGTACGTGCTGTACTAAACATTC

mRNA sequence

CTTCTCCGAACGAACAATGAAGTGCGTGGAATTCGTTCTTTTTTCCACTTTCCATTTCCCTCCACTGCTTATATCTTATTCAAATCTTCTCTTACTTGTTTAATCAACCTTTTCAATCGTCTTTTCTTCCATTTTCCGCTTCTAACTTCGATCGCCATGTCTAAAAGGGTTCTATGCAAGTATTTTGCGCATGGAGCATGCTTGAAAGGGGAGTATTGTGAGTTTTCGCATGACTGGACGGCTCCACCTAGCAATGTTTGCACTTACTATCAAAAGGGAAGGTGTGCTTTTGGCAGTCGATGCAGATATGACCATGTTAAAGTTTCTCATGCAGAGTCATCAGCTTCTTCTAATTCTCAGCAACCTCACGTGTCTGAATCTGTTTCAATAGCTCATACTCTATTATCAAGTCCACTTTCTCCTTCACCTGGGTCGACAATTGAGCTTGCTGGTTCAAAGATATCCTGTCTTCCCTCGCACGTTGAATCTGCTTGGAGTGTAGAGTGTGAGCTTCCATCTGGTCTTGATGATTGGGGGAGTAGGGAGACAAGGATTATTAGTCCAGCCGATTTACCTATTTGCTCTTTTTCTGCTGCTGGTACTAATTGTCCGCGTGGTGATAATTGCCCTCACATTCATGGGGACTTATGTCCCACGTGCGGAAAACAATGTTTACATCCTTTTAGGACGGATGAAAGGGAGGAGCATATGAAGGCATGTGAGAAAAAGCAAAAACATCTCGAGTCTTTGAAACATAGCCAAGAAATAGAGTGTAGTGTCTGCCTCGAGCGTGTTCTTTCTAAAACCGAAATAGCCGAGAGGAAGTTTGGGCTTCTATCTGAATGTGATCATCCGTTCTGCATTTCTTGTATTAGAAACTGGCGTAATAGTTCATCTACCTCCGGAATGGATGTTAACAGTGCTTTAAGAGCCTGCCCAATTTGTAGGAAGCTATCATACTTTGTTATTCCAAGCGTCATTTGGTATTCTTCCAAGGAAGAAAAACAAGAGATCGTTGATAGCTACAAGTCAAAGCTGCGATCAATCGATTGCAAGCACTTTAACTTTGGAAATGGGAACTGCCCATTTGGATCTAGCTGTTTTTACAAGCACTTGGTCAAGCCTGGTTCATACACATGGAAATATCACAGGCCACCTCTGCTCCGACCACGATCACAGAGAACTCCTCCTAGCAGATCTAATATTGTGGATATGGATGCCTTTTTTGATATGTTTGAAGACTTAGAGGATTTGGATTTTAGTGCTGAAGATTTAGATTTTGAAGATTTAACACCTTTGGAGATGGCATTTGTTCTAATGCAGTTGGGTTGTGAAGCCAACTTGTCAAGTGATGAGGAGAATTATATTGTGCCTGATCACGCACATCGGGATGGCCGGTTGGAAGAGGTAGCACTGCGTCACATTGGGAATCAAGACGGCCTCACTATTATAGCTAAAAACATTAGGTTGTCGGACTTCCTTAGTGACTTGCATATCAGGTGTCGAATTGCTTGCTGGAGTCCGATAATGAGAAATGGAAGGTGGGTTATTGAACACGGTGATTCGAACTTCATCATCTTCTATGAATGGAAATAAAGCTAAAGACATTGTCATACTGAGAAATGAATGTAGTAAGTTGAAGCCTTATTCTTGGGATGGCAAAATAAAGGGTTTTTCACATCAGATGTAGCACAGCTGAATAGCTCAATAAATTGCGTCGTTGGTTGTACGTGCTGTACTAAACATTC

Coding sequence (CDS)

ATGTCTAAAAGGGTTCTATGCAAGTATTTTGCGCATGGAGCATGCTTGAAAGGGGAGTATTGTGAGTTTTCGCATGACTGGACGGCTCCACCTAGCAATGTTTGCACTTACTATCAAAAGGGAAGGTGTGCTTTTGGCAGTCGATGCAGATATGACCATGTTAAAGTTTCTCATGCAGAGTCATCAGCTTCTTCTAATTCTCAGCAACCTCACGTGTCTGAATCTGTTTCAATAGCTCATACTCTATTATCAAGTCCACTTTCTCCTTCACCTGGGTCGACAATTGAGCTTGCTGGTTCAAAGATATCCTGTCTTCCCTCGCACGTTGAATCTGCTTGGAGTGTAGAGTGTGAGCTTCCATCTGGTCTTGATGATTGGGGGAGTAGGGAGACAAGGATTATTAGTCCAGCCGATTTACCTATTTGCTCTTTTTCTGCTGCTGGTACTAATTGTCCGCGTGGTGATAATTGCCCTCACATTCATGGGGACTTATGTCCCACGTGCGGAAAACAATGTTTACATCCTTTTAGGACGGATGAAAGGGAGGAGCATATGAAGGCATGTGAGAAAAAGCAAAAACATCTCGAGTCTTTGAAACATAGCCAAGAAATAGAGTGTAGTGTCTGCCTCGAGCGTGTTCTTTCTAAAACCGAAATAGCCGAGAGGAAGTTTGGGCTTCTATCTGAATGTGATCATCCGTTCTGCATTTCTTGTATTAGAAACTGGCGTAATAGTTCATCTACCTCCGGAATGGATGTTAACAGTGCTTTAAGAGCCTGCCCAATTTGTAGGAAGCTATCATACTTTGTTATTCCAAGCGTCATTTGGTATTCTTCCAAGGAAGAAAAACAAGAGATCGTTGATAGCTACAAGTCAAAGCTGCGATCAATCGATTGCAAGCACTTTAACTTTGGAAATGGGAACTGCCCATTTGGATCTAGCTGTTTTTACAAGCACTTGGTCAAGCCTGGTTCATACACATGGAAATATCACAGGCCACCTCTGCTCCGACCACGATCACAGAGAACTCCTCCTAGCAGATCTAATATTGTGGATATGGATGCCTTTTTTGATATGTTTGAAGACTTAGAGGATTTGGATTTTAGTGCTGAAGATTTAGATTTTGAAGATTTAACACCTTTGGAGATGGCATTTGTTCTAATGCAGTTGGGTTGTGAAGCCAACTTGTCAAGTGATGAGGAGAATTATATTGTGCCTGATCACGCACATCGGGATGGCCGGTTGGAAGAGGTAGCACTGCGTCACATTGGGAATCAAGACGGCCTCACTATTATAGCTAAAAACATTAGGTTGTCGGACTTCCTTAGTGACTTGCATATCAGGTGTCGAATTGCTTGCTGGAGTCCGATAATGAGAAATGGAAGGTGGGTTATTGAACACGGTGATTCGAACTTCATCATCTTCTATGAATGGAAATAA

Protein sequence

MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAESSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELPSGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMFEDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAHRDGRLEEVALRHIGNQDGLTIIAKNIRLSDFLSDLHIRCRIACWSPIMRNGRWVIEHGDSNFIIFYEWK
BLAST of Carg13450 vs. NCBI nr
Match: XP_022959900.1 (E3 ubiquitin-protein ligase makorin-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 786.9 bits (2031), Expect = 3.7e-224
Identity = 400/403 (99.26%), Postives = 401/403 (99.50%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
           SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGS+ISCLPSHVESAWSVECELP
Sbjct: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSRISCLPSHVESAWSVECELP 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
           SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR
Sbjct: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK
Sbjct: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPP  RPRSQRTPPSRSNIVDMDAFFDMF
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPPRPRSQRTPPSRSNIVDMDAFFDMF 360

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 404
           EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY
Sbjct: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 403

BLAST of Carg13450 vs. NCBI nr
Match: XP_023514215.1 (E3 ubiquitin-protein ligase makorin-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 785.0 bits (2026), Expect = 1.4e-223
Identity = 400/403 (99.26%), Postives = 400/403 (99.26%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
           SSASSNSQQPHVSESVSIAHTLLSSPLSPSP STIELAGSKISCLPSHVESAWSVECELP
Sbjct: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPESTIELAGSKISCLPSHVESAWSVECELP 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
           SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR
Sbjct: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK
Sbjct: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPP  RPRSQRTPPSRSNIVDMDAFFDMF
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPPRPRSQRTPPSRSNIVDMDAFFDMF 360

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 404
           EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY
Sbjct: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 403

BLAST of Carg13450 vs. NCBI nr
Match: XP_023004552.1 (E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 782.7 bits (2020), Expect = 7.0e-223
Identity = 397/403 (98.51%), Postives = 400/403 (99.26%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVK SHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKASHAE 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
           SSASSNSQQPH+SESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP
Sbjct: 61  SSASSNSQQPHMSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
           SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR
Sbjct: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK
Sbjct: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPP  RPRSQRTPPSRSNIVDMDAFFDMF
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPHRPRSQRTPPSRSNIVDMDAFFDMF 360

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 404
           +DLEDLDFSAEDLDFE+LTPLEMAFVLMQLGCEANLSSDEENY
Sbjct: 361 QDLEDLDFSAEDLDFEELTPLEMAFVLMQLGCEANLSSDEENY 403

BLAST of Carg13450 vs. NCBI nr
Match: XP_022959901.1 (E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 779.2 bits (2011), Expect = 7.8e-222
Identity = 396/399 (99.25%), Postives = 397/399 (99.50%), Query Frame = 0

Query: 5   VLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAESSAS 64
           VLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAESSAS
Sbjct: 5   VLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAESSAS 64

Query: 65  SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELPSGLD 124
           SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGS+ISCLPSHVESAWSVECELPSGLD
Sbjct: 65  SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSRISCLPSHVESAWSVECELPSGLD 124

Query: 125 DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH 184
           DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH
Sbjct: 125 DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH 184

Query: 185 MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN 244
           MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN
Sbjct: 185 MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN 244

Query: 245 SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF 304
           SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF
Sbjct: 245 SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF 304

Query: 305 GNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMFEDLE 364
           GNGNCPFGSSCFYKHLVKPGSYTWKYHRPP  RPRSQRTPPSRSNIVDMDAFFDMFEDLE
Sbjct: 305 GNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPPRPRSQRTPPSRSNIVDMDAFFDMFEDLE 364

Query: 365 DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 404
           DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY
Sbjct: 365 DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 403

BLAST of Carg13450 vs. NCBI nr
Match: XP_022959902.1 (E3 ubiquitin-protein ligase makorin-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 779.2 bits (2011), Expect = 7.8e-222
Identity = 395/399 (99.00%), Postives = 396/399 (99.25%), Query Frame = 0

Query: 5   VLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAESSAS 64
           VLCKYFAHGACLKGEYCEFSHDWTAPPSN XXXXXXXXXXXXXXXXXXXVKVSHAESSAS
Sbjct: 3   VLCKYFAHGACLKGEYCEFSHDWTAPPSNVXXXXXXXXXXXXXXXXXXXVKVSHAESSAS 62

Query: 65  SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELPSGLD 124
           SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGS+ISCLPSHVESAWSVECELPSGLD
Sbjct: 63  SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSRISCLPSHVESAWSVECELPSGLD 122

Query: 125 DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH 184
           DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH
Sbjct: 123 DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH 182

Query: 185 MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN 244
           MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN
Sbjct: 183 MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN 242

Query: 245 SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF 304
           SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF
Sbjct: 243 SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF 302

Query: 305 GNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMFEDLE 364
           GNGNCPFGSSCFYKHLVKPGSYTWKYHRPP  RPRSQRTPPSRSNIVDMDAFFDMFEDLE
Sbjct: 303 GNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPPRPRSQRTPPSRSNIVDMDAFFDMFEDLE 362

Query: 365 DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 404
           DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY
Sbjct: 363 DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 401

BLAST of Carg13450 vs. TAIR10
Match: AT3G08505.2 (zinc finger (CCCH-type/C3HC4-type RING finger) family protein)

HSP 1 Score: 370.5 bits (950), Expect = 1.5e-102
Identity = 223/447 (49.89%), Postives = 265/447 (59.28%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE 60
           MS R+LCK+F HG+CLKGE CEFSHD   PP+N XXXXXXXXXXXXXXXXXXXV+     
Sbjct: 1   MSDRILCKFFVHGSCLKGENCEFSHDSKDPPNNVXXXXXXXXXXXXXXXXXXXVR----- 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
             A+SN      SES       L   +S +P   ++  G       +  + + +V C   
Sbjct: 61  --AASNLPLSSDSES-------LDRSISTTPSRHLQQQGDN-----NDGDKSSNVYC--- 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
                        I P + PICSF+AAG +CPRG+ CPH+HGDLC TCGK+CLHPFR +E
Sbjct: 121 -------------IHPREYPICSFAAAG-DCPRGNQCPHMHGDLCNTCGKKCLHPFRPEE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEH K CEKKQKH+E+LK SQ+IECSVCL+R+LSK    ERKFGLL+ECDHPFCI CIR
Sbjct: 181 REEHTKECEKKQKHIEALKQSQDIECSVCLDRILSKATPGERKFGLLTECDHPFCIQCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWR+S+  SGMDVNS LRACPICRKLSYFV+PSV+WYSS EEK+EI+D YK+KLRSIDCK
Sbjct: 241 NWRSSAPVSGMDVNSTLRACPICRKLSYFVVPSVVWYSSPEEKKEIIDIYKAKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFG+SCFYK                                          
Sbjct: 301 HFNFGNGNCPFGASCFYK------------------------------------------ 322

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAHRDGRLEEVAL 420
                                                          HA+ DG LEEV L
Sbjct: 361 -----------------------------------------------HAYSDGHLEEVVL 322

Query: 421 RHIGNQDGLTIIAKNIRLSDFLSDLHI 448
           RH+G+Q+G T+I  +IRLS+FL  L I
Sbjct: 421 RHLGSQEGETVITDSIRLSEFLGGLQI 322

BLAST of Carg13450 vs. Swiss-Prot
Match: sp|Q5ZA07|C3H41_ORYSJ (E3 ubiquitin-protein ligase makorin OS=Oryza sativa subsp. japonica OX=39947 GN=MKRN PE=2 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 8.2e-106
Identity = 239/457 (52.30%), Postives = 267/457 (58.42%), Query Frame = 0

Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAES 61
           +KRVLCK+F HGACLKGEYCEFSHDW   P+NXXXXXXXXXXXXXXXXXXXXVKVS   +
Sbjct: 3   TKRVLCKFFMHGACLKGEYCEFSHDWNDQPNNXXXXXXXXXXXXXXXXXXXXVKVSRNPT 62

Query: 62  SASSNSQQPHVSESVSIAHTLLSSPL-------SPSPGSTIELAGSKISCLPSHVESAWS 121
                           +       P        S +P   I +     S       + + 
Sbjct: 63  VXXXXXXXXXXXXXXXLQPLSFGRPHHVGYQADSSNPRQQISMDVLAHSGSKPVWRNDFQ 122

Query: 122 VECELPSGLDDWGSRET----RIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGK 181
            E  L  G+ DW    T      +SPADLPICSF AAG NCP G+ CP +HGDLC TCGK
Sbjct: 123 HESVLEDGI-DWSISPTVQNQTTLSPADLPICSF-AAGGNCPYGEECPQMHGDLCTTCGK 182

Query: 182 QCLHPFRTDEREEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSEC 241
            CLHP+R DEREEH K CEK  K LESLK SQEIECSVCL+RVLSK   AERKFGLLSEC
Sbjct: 183 MCLHPYRPDEREEHTKLCEKNHKRLESLKRSQEIECSVCLDRVLSKPTAAERKFGLLSEC 242

Query: 242 DHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSY 301
           DHPFCISCIRNWRN+S TSGMDVNSALRACPICRKLSY+VIPSV+WY SKEEK EI+D+Y
Sbjct: 243 DHPFCISCIRNWRNNSPTSGMDVNSALRACPICRKLSYYVIPSVLWYFSKEEKLEIIDNY 302

Query: 302 KSKLRSIDCKHFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNI 361
           K+KL+SIDCK+F+FG G CPFGSSCFYK                                
Sbjct: 303 KAKLKSIDCKYFDFGTGTCPFGSSCFYK-------------------------------- 362

Query: 362 VDMDAFFDMFEDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAH 421
                                                                    HA+
Sbjct: 363 ---------------------------------------------------------HAY 368

Query: 422 RDGRLEEVALRHIGNQDGLTIIAKNIRLSDFLSDLHI 448
           RDGRLEEV LRH+   DG T+IAKNIRLSDFLS LH+
Sbjct: 423 RDGRLEEVILRHLDADDGSTVIAKNIRLSDFLSRLHL 368

BLAST of Carg13450 vs. Swiss-Prot
Match: sp|E0X9N4|C3H69_ARATH (Putative RING-type E3 ubiquitin transferase C3H69 OS=Arabidopsis thaliana OX=3702 GN=At3g63550 PE=3 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 3.1e-105
Identity = 229/448 (51.12%), Postives = 275/448 (61.38%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE 60
           MSKR+LCK+FAHGACLKGE+CEFSHDW  P +NXXXXXXXXXXXXXXXXXXXXVK S   
Sbjct: 1   MSKRILCKFFAHGACLKGEHCEFSHDWKDPTNNXXXXXXXXXXXXXXXXXXXXVKASRPH 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLP-SHVESAWSVECEL 120
                              +TL  + L   PG    +A   +S +P S ++  W+++   
Sbjct: 61  -XXXXXXXXXXXXXXXXXXNTLACAFL---PG----VAERDLSPVPSSSLKPTWNLDSLH 120

Query: 121 PSGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTD 180
              +D+  +     + P +LPICSF+AAG +CPRG+ CPHIHG +CPTCGK CLHPFR D
Sbjct: 121 QDSVDEVKTSNPGTVKPEELPICSFAAAG-DCPRGNECPHIHGTICPTCGKCCLHPFRPD 180

Query: 181 EREEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCI 240
           EREEH K CEKK K LE+LK SQEIEC VCLERVLSK   AERKFGLL+ECDH FCI+CI
Sbjct: 181 EREEHKKVCEKKHKQLEALKLSQEIECCVCLERVLSKATPAERKFGLLTECDHAFCIACI 240

Query: 241 RNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDC 300
           RNWR+SS ++GMDVNS LRACPICRKLSYFV+PSVIW+S+ EEK+EI+D+Y+ KLRSIDC
Sbjct: 241 RNWRSSSPSTGMDVNSTLRACPICRKLSYFVVPSVIWFSAPEEKKEIMDNYREKLRSIDC 300

Query: 301 KHFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDM 360
           KHF+FG+GNCPFG+SCFYK                                         
Sbjct: 301 KHFSFGDGNCPFGTSCFYK----------------------------------------- 350

Query: 361 FEDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAHRDGRLEEVA 420
                                                           HA+ DGRLEEV 
Sbjct: 361 ------------------------------------------------HAYHDGRLEEVV 350

Query: 421 LRHIGNQDGLTIIAKNIRLSDFLSDLHI 448
           LRH+  +DG T+IAK+IRLSDFL ++HI
Sbjct: 421 LRHLDAEDGQTVIAKDIRLSDFLENMHI 350

BLAST of Carg13450 vs. Swiss-Prot
Match: sp|Q6IDS6|C3H35_ARATH (E3 ubiquitin-protein ligase makorin OS=Arabidopsis thaliana OX=3702 GN=MKRN PE=2 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 2.7e-101
Identity = 223/447 (49.89%), Postives = 265/447 (59.28%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE 60
           MS R+LCK+F HG+CLKGE CEFSHD   PP+N XXXXXXXXXXXXXXXXXXXV+     
Sbjct: 1   MSDRILCKFFVHGSCLKGENCEFSHDSKDPPNNVXXXXXXXXXXXXXXXXXXXVR----- 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
             A+SN      SES       L   +S +P   ++  G       +  + + +V C   
Sbjct: 61  --AASNLPLSSDSES-------LDRSISTTPSRHLQQQGDN-----NDGDKSSNVYC--- 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
                        I P + PICSF+AAG +CPRG+ CPH+HGDLC TCGK+CLHPFR +E
Sbjct: 121 -------------IHPREYPICSFAAAG-DCPRGNQCPHMHGDLCNTCGKKCLHPFRPEE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEH K CEKKQKH+E+LK SQ+IECSVCL+R+LSK    ERKFGLL+ECDHPFCI CIR
Sbjct: 181 REEHTKECEKKQKHIEALKQSQDIECSVCLDRILSKATPGERKFGLLTECDHPFCIQCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWR+S+  SGMDVNS LRACPICRKLSYFV+PSV+WYSS EEK+EI+D YK+KLRSIDCK
Sbjct: 241 NWRSSAPVSGMDVNSTLRACPICRKLSYFVVPSVVWYSSPEEKKEIIDIYKAKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFG+SCFYK                                          
Sbjct: 301 HFNFGNGNCPFGASCFYK------------------------------------------ 322

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAHRDGRLEEVAL 420
                                                          HA+ DG LEEV L
Sbjct: 361 -----------------------------------------------HAYSDGHLEEVVL 322

Query: 421 RHIGNQDGLTIIAKNIRLSDFLSDLHI 448
           RH+G+Q+G T+I  +IRLS+FL  L I
Sbjct: 421 RHLGSQEGETVITDSIRLSEFLGGLQI 322

BLAST of Carg13450 vs. Swiss-Prot
Match: sp|Q8JFF3|MKRN1_SERQU (Probable E3 ubiquitin-protein ligase makorin-1 OS=Seriola quinqueradiata OX=8161 GN=mkrn1 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 4.3e-46
Identity = 138/399 (34.59%), Postives = 214/399 (53.63%), Query Frame = 0

Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDWT--APPSNXXXXXXXXXXXXXXXXXXXXVKVSHA 61
           +K V C+YF HG C +G+ C +SHD T   P + XXXXXXXXXXXXXXXXXXXX K    
Sbjct: 19  TKHVTCRYFMHGLCKEGDNCRYSHDLTNSKPAAXXXXXXXXXXXXXXXXXXXXXCK---- 78

Query: 62  ESSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSK-----ISCLPSHVESAWS 121
               + N + P   + + +    L+ P  P P     + G++        +P       +
Sbjct: 79  ---PAKNEELP-APQMLPLPSASLAGPSDPEPSGPTPVPGAQDWVNAAEFVPGQPYCGRA 138

Query: 122 VECELPSG---LDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQ 181
            + ++ S    ++++ S            +C ++A G  C  G NC ++HGD+C  CG Q
Sbjct: 139 EQAKVESSVPLIEEFDSYPAPDNKQLRKQLCPYAAVG-ECRYGINCAYLHGDVCYMCGLQ 198

Query: 182 CLHPFRTDEREEHMKAC-EKKQKHLE---SLKHSQEIECSVCLERVLSKTEIAERKFGLL 241
            LHP   ++R EH KAC E  +K +E   +++ S+++ C VC+E V  K   +ER+FG+L
Sbjct: 199 VLHPTDNNQRSEHTKACIEAHEKDMEISFAIQRSKDMMCGVCMEVVFEKANPSERRFGIL 258

Query: 242 SECDHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIV 301
           S C H +C+ CIR WR++        +  +++CP CR  S FVIPS  W   K++KQ+++
Sbjct: 259 SNCSHCYCLKCIRKWRSAKQFE----SKIIKSCPECRITSNFVIPSEYWVEDKDDKQKLI 318

Query: 302 DSYKSKLRSIDCKHFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRT-PPS 361
             YK  + S  C++F+ G G CPFGS+CFYKH    G       R    +P+ ++T   S
Sbjct: 319 QKYKDGMGSKPCRYFDEGRGTCPFGSNCFYKHAFPDG-------RLEEAQPQRRQTGSNS 378

Query: 362 RSNIVDMDAFFDMFEDLEDLD-FSAEDLDFEDLTPLEMA 385
           R+        +D++++ E  D F  ED   E++   E++
Sbjct: 379 RNRNSRRTPLWDIYDERESTDSFDNED---EEMVTFELS 394

BLAST of Carg13450 vs. Swiss-Prot
Match: sp|Q4VBT5|MKRN1_DANRE (Probable E3 ubiquitin-protein ligase makorin-1 OS=Danio rerio OX=7955 GN=mkrn1 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 5.6e-46
Identity = 129/341 (37.83%), Postives = 187/341 (54.84%), Query Frame = 0

Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAES 61
           +K V C+YF HG C +GE C +SHD ++    XXXXXXXXXXXXXXXXXXXX      + 
Sbjct: 19  TKHVTCRYFMHGLCKEGENCRYSHDLSSCKQXXXXXXXXXXXXXXXXXXXXXXXXXXKQD 78

Query: 62  SASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIEL--------------AGSKISCLPS 121
              S+     ++ +  +A T    P+S  PG T                 A   +   P 
Sbjct: 79  EVPSSKPSMPLT-AAPLAGT--PEPVSDGPGGTTGAQEKPQGSGAVDWVNAAEFVPGQP- 138

Query: 122 HVESAWSVECELPSGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPT 181
           +   A  V CE P  L +    + +        +C ++A G  C  G NC ++HGD+C  
Sbjct: 139 YCGRADPVLCEGPGPLIEEEYEKEQANKEMKKQLCPYAAVG-ECRYGLNCAYLHGDVCDM 198

Query: 182 CGKQCLHPFRTDEREEHMKAC-EKKQKHLE---SLKHSQEIECSVCLERVLSKTEIAERK 241
           CG Q LHP  T +R +H++AC E  +K +E   +++ S+++ C VC+E V  KT  +ER+
Sbjct: 199 CGLQVLHPSDTSQRSQHIRACIEAHEKDMEISFAIQRSKDMMCGVCMEVVFEKTNPSERR 258

Query: 242 FGLLSECDHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEK 301
           FG+LS C H +C+ CIR WR++        +  +++CP CR  S FVIPS  W   KEEK
Sbjct: 259 FGILSNCCHCYCLKCIRKWRSAKQFE----SKIIKSCPECRITSNFVIPSEYWVEDKEEK 318

Query: 302 QEIVDSYKSKLRSIDCKHFNFGNGNCPFGSSCFYKHLVKPG 325
           Q+++  YK  + +  C++F+ G G CPFG++CFYKH    G
Sbjct: 319 QQLIQKYKDGMGTKPCRYFDEGRGTCPFGANCFYKHAFPDG 350

BLAST of Carg13450 vs. TrEMBL
Match: tr|A0A0A0KBH2|A0A0A0KBH2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G428290 PE=4 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 5.4e-187
Identity = 344/402 (85.57%), Postives = 364/402 (90.55%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
           S   SN QQ H SESVS+AH+ LS PLSP  GST ELA S+I  LP   E+AWS E   P
Sbjct: 61  S--CSNVQQSHGSESVSVAHSQLSRPLSPFSGSTSELASSEIPSLPLREEAAWSGEFVRP 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
            GLDDW + E R++ PAD+PICSF+AAG +CPRG+NCPHIHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 DGLDDWVNGEARVVGPADIPICSFAAAGGDCPRGENCPHIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEH+KACEKKQKHLESLKHSQ+IECSVCLERVLSKTE AERKFG+LSECDHPFCISCIR
Sbjct: 181 REEHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWRNSSSTSGMD+NSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKS+LRSIDCK
Sbjct: 241 NWRNSSSTSGMDINSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYKHLV PGSYTWKYHRPP  +P+S RTPPSRSNIVDMDAFF+MF
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVIPGSYTWKYHRPPPRQPQSWRTPPSRSNIVDMDAFFEMF 360

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEEN 403
           EDLEDLDFS EDLDFE LT LEMA  LMQLGCEAN SSDEE+
Sbjct: 361 EDLEDLDFSDEDLDFEYLTLLEMA--LMQLGCEANSSSDEES 398

BLAST of Carg13450 vs. TrEMBL
Match: tr|A0A1S3CEC1|A0A1S3CEC1_CUCME (E3 ubiquitin-protein ligase makorin OS=Cucumis melo OX=3656 GN=LOC103499913 PE=4 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 3.1e-158
Identity = 309/448 (68.97%), Postives = 330/448 (73.66%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSH E
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHTE 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
           S   SN QQ H SES+S+AH+ LS PLSP  GST ELA S++  LP   E+AWS E   P
Sbjct: 61  S--CSNVQQSHGSESLSVAHSQLSRPLSPFSGSTSELASSEMPSLPLRHETAWSGEFVRP 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
            GLDDWG+ E R++ PAD+PICSF+AAG +CPRG+NCP IHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 DGLDDWGNGEARVVGPADIPICSFAAAGGDCPRGENCPRIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEH+KACEKKQKHLESLKHSQ+IECSVCLERVLSKTE AERKFG+LSECDHPFCISCIR
Sbjct: 181 REEHLKACEKKQKHLESLKHSQDIECSVCLERVLSKTETAERKFGILSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKS+LRSIDCK
Sbjct: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSRLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYK                                          
Sbjct: 301 HFNFGNGNCPFGSSCFYK------------------------------------------ 357

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAHRDGRLEEVAL 420
                                                          HAHRDGRLEE+AL
Sbjct: 361 -----------------------------------------------HAHRDGRLEEIAL 357

Query: 421 RHIGNQDGLTIIAKNIRLSDFLSDLHIR 449
           RHIGN+DGLT+IAKN+RLSDFLSDLHIR
Sbjct: 421 RHIGNEDGLTVIAKNLRLSDFLSDLHIR 357

BLAST of Carg13450 vs. TrEMBL
Match: tr|A0A061E386|A0A061E386_THECC (Zinc finger family protein isoform 1 OS=Theobroma cacao OX=3641 GN=TCM_005895 PE=4 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.1e-150
Identity = 304/469 (64.82%), Postives = 356/469 (75.91%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE 60
           M KRVLCK+FAHGACLKGE+CEFSHDW  PP+ XXXXXXXXXXXXXXXXXXXXV+ S ++
Sbjct: 1   MPKRVLCKFFAHGACLKGEHCEFSHDWKDPPNXXXXXXXXXXXXXXXXXXXXXVRASQSD 60

Query: 61  SSASSNS---QQPHVSESVSIAHTLLSS--PLSPSPGSTIELAGSKISCLPSHVESAWSV 120
            SASS+S   +Q  +S+SV +  +L ++       P ++ E  GS  + LP   + AW++
Sbjct: 61  LSASSSSTVLRQSLISDSVPLGPSLTTTFGGSVVHPSASAEFPGSSRAFLPP-TKQAWNL 120

Query: 121 ECELPSGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHP 180
           E E  +  D+    E R ++PA+  ICSF+AAG NCPRG+ C HIHGDLC TCGK CLHP
Sbjct: 121 ESEHQALSDNRDFVEPRRVNPAERSICSFAAAG-NCPRGEKCAHIHGDLCATCGKHCLHP 180

Query: 181 FRTDEREEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFC 240
           FR  EREEH+K CEKKQKHLE+LK SQEIECSVCL+RVLSK   AERKFGLLSECDHPFC
Sbjct: 181 FRPQEREEHIKMCEKKQKHLEALKRSQEIECSVCLDRVLSKPTAAERKFGLLSECDHPFC 240

Query: 241 ISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLR 300
           ISCIRNWR+SS +SGMDVN+ALR CPICRKLSYFVIPSVIWY + EEKQEIVDSYK+KLR
Sbjct: 241 ISCIRNWRSSSPSSGMDVNTALRTCPICRKLSYFVIPSVIWYCTPEEKQEIVDSYKAKLR 300

Query: 301 SIDCKHFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDA 360
           SIDCKHFNFGNGNCPFG+SCFYKH VKPGSY WKYHRP          PP R N +DMDA
Sbjct: 301 SIDCKHFNFGNGNCPFGTSCFYKHTVKPGSYVWKYHRP----------PPRRFNFMDMDA 360

Query: 361 FFDMFEDL---EDLD-FSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPD---- 420
            FD+FE L   +++D F AED +++DLTPLEMAF+LMQ   E+  SS   + + P     
Sbjct: 361 IFDVFEQLIAEDEMDIFDAEDFEYDDLTPLEMAFLLMQQDFESMRSSWCFDRLPPFLSGR 420

Query: 421 ---------HAHRDGRLEEVALRHIGNQDGLTIIAKNIRLSDFLSDLHI 448
                    HA+RDGRLEEV LRH+G +DG TIIAKNIRLSD+LSDLHI
Sbjct: 421 RWILEFVNLHAYRDGRLEEVVLRHLGAEDGHTIIAKNIRLSDYLSDLHI 457

BLAST of Carg13450 vs. TrEMBL
Match: tr|A0A2H5N9V0|A0A2H5N9V0_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_025730 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 9.3e-147
Identity = 276/485 (56.91%), Postives = 328/485 (67.63%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE 60
           MSKRVLCK+FAHGACLKGE+CEFSHDW  PP+N                    VK S +E
Sbjct: 1   MSKRVLCKFFAHGACLKGEHCEFSHDWKDPPNNICTYYQKGFCSYASRCRYEHVKPSRSE 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
           S+ASS+S   H S +        SS ++  PG   EL+      LP + ++AW+ E    
Sbjct: 61  SAASSSSSVSHPSRAT-------SSGITKVPGVMPELSALSRPFLPPN-KTAWNPESVCN 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
             L++    E R + PAD  ICSF+AAG NCPRG+ CPHIHGD CPTCGKQCLHPFR +E
Sbjct: 121 DSLENDEVDEPRNLKPADRSICSFAAAG-NCPRGEKCPHIHGDTCPTCGKQCLHPFRPEE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEHMK+CEKKQKHLE+L+ SQEIECSVCL+RVLSK   AERKFGLLSECDHPFCISCIR
Sbjct: 181 REEHMKSCEKKQKHLEALRRSQEIECSVCLDRVLSKPTAAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWR+SS TSGMDVN+ALRACPICRKLSYFVIPSVIWY + EEKQEI+DSYKSKL+SIDCK
Sbjct: 241 NWRSSSPTSGMDVNTALRACPICRKLSYFVIPSVIWYYTPEEKQEIIDSYKSKLKSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFG+SCFYKH+VKPGSY WKYHRPP             SN+VDMD+F D F
Sbjct: 301 HFNFGNGNCPFGTSCFYKHMVKPGSYMWKYHRPPPXXXXXXXXXXXXSNVVDMDSFIDAF 360

Query: 361 EDL---EDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEE---------------- 420
            DL   ++ DF   + +      +EMA +L QL  +++ SSD E                
Sbjct: 361 GDLLGEDEPDFFDAENEVRMALDVEMALLLTQLDFDSSDSSDYEIIRTRYDISSPFCVGH 420

Query: 421 ------------------NYIVPDHAHRDGRLEEVALRHIGNQDGLTIIAKNIRLSDFLS 449
                             N +   HA+ DGRLEEV LRH+G+ DG T+IAK+IRLS+FL+
Sbjct: 421 LSCSSSWCFDSPLHFASWNKMKLLHAYTDGRLEEVVLRHLGSDDGSTVIAKDIRLSEFLT 476

BLAST of Carg13450 vs. TrEMBL
Match: tr|A0A2P5E715|A0A2P5E715_9ROSA (Cdk-activating kinase assembly factor OS=Trema orientalis OX=63057 GN=TorRG33x02_228760 PE=4 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 2.7e-138
Identity = 274/411 (66.67%), Postives = 323/411 (78.59%), Query Frame = 0

Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNXXXXXXXXXXXXXXXXXXXXVKVSHAE 60
           MSKRVLCK+FAHGACLKGE+CE+SHDW  PP+NXXXXXXXXXXXXXXXXXXXX K   A+
Sbjct: 1   MSKRVLCKFFAHGACLKGEHCEYSHDWKDPPNNXXXXXXXXXXXXXXXXXXXXXKAERAQ 60

Query: 61  SSASSN---SQQPHVSESVSIAHTLLSSP--LSPSPGSTIELAG-SKISCLPSHVESAWS 120
           SSASS+   SQQ  VS+S S+ H+  +S   ++  PG+  EL+  S+   LP     AW 
Sbjct: 61  SSASSSSTISQQFVVSDSTSLPHSSSTSSKGIAFIPGAVSELSALSRPFVLPK--PPAWK 120

Query: 121 VECELPSGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLH 180
            E E    LD     ET  ++PADLPICSF+AAG +CPRG+ CPH+HGDLCPTCGK CLH
Sbjct: 121 QEYEQSHFLDGGDVGETS-VTPADLPICSFAAAG-DCPRGEKCPHVHGDLCPTCGKHCLH 180

Query: 181 PFRTDEREEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPF 240
           PFR DE+EEH++ C KK KHLE+LKHSQEIECSVCLERVLSK   AERKFGL+SECDHPF
Sbjct: 181 PFRPDEKEEHLRTCNKKHKHLEALKHSQEIECSVCLERVLSKPTAAERKFGLMSECDHPF 240

Query: 241 CISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKL 300
           CISCIRNWR+SS TSG DVNSALRACPICRKLSYFV+PSVIWY+SKEEKQEIV+SYK+KL
Sbjct: 241 CISCIRNWRSSSPTSGFDVNSALRACPICRKLSYFVVPSVIWYTSKEEKQEIVNSYKAKL 300

Query: 301 RSIDCKHFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMD 360
           +SIDCKHF+FGNGNCPFG+SCFY+H VKPGSY WK+HRPP  RP      P RSN+V+MD
Sbjct: 301 KSIDCKHFDFGNGNCPFGTSCFYRHTVKPGSYVWKHHRPPPRRP-----TPRRSNVVEMD 360

Query: 361 AFFDMFEDLEDLD-FSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYI 405
           A  DMFE L++++ +S EDLD ED  P EM  +   +G + + SS +E +I
Sbjct: 361 ALLDMFEHLDEMECYSPEDLDSEDTNPWEMGLLFSLMGFDTSDSSSDEEFI 402

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022959900.13.7e-22499.26E3 ubiquitin-protein ligase makorin-like isoform X1 [Cucurbita moschata][more]
XP_023514215.11.4e-22399.26E3 ubiquitin-protein ligase makorin-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023004552.17.0e-22398.51E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita maxima][more]
XP_022959901.17.8e-22299.25E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita moschata][more]
XP_022959902.17.8e-22299.00E3 ubiquitin-protein ligase makorin-like isoform X3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G08505.21.5e-10249.89zinc finger (CCCH-type/C3HC4-type RING finger) family protein[more]
Match NameE-valueIdentityDescription
sp|Q5ZA07|C3H41_ORYSJ8.2e-10652.30E3 ubiquitin-protein ligase makorin OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
sp|E0X9N4|C3H69_ARATH3.1e-10551.12Putative RING-type E3 ubiquitin transferase C3H69 OS=Arabidopsis thaliana OX=370... [more]
sp|Q6IDS6|C3H35_ARATH2.7e-10149.89E3 ubiquitin-protein ligase makorin OS=Arabidopsis thaliana OX=3702 GN=MKRN PE=2... [more]
sp|Q8JFF3|MKRN1_SERQU4.3e-4634.59Probable E3 ubiquitin-protein ligase makorin-1 OS=Seriola quinqueradiata OX=8161... [more]
sp|Q4VBT5|MKRN1_DANRE5.6e-4637.83Probable E3 ubiquitin-protein ligase makorin-1 OS=Danio rerio OX=7955 GN=mkrn1 P... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0KBH2|A0A0A0KBH2_CUCSA5.4e-18785.57Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G428290 PE=4 SV=1[more]
tr|A0A1S3CEC1|A0A1S3CEC1_CUCME3.1e-15868.97E3 ubiquitin-protein ligase makorin OS=Cucumis melo OX=3656 GN=LOC103499913 PE=4... [more]
tr|A0A061E386|A0A061E386_THECC1.1e-15064.82Zinc finger family protein isoform 1 OS=Theobroma cacao OX=3641 GN=TCM_005895 PE... [more]
tr|A0A2H5N9V0|A0A2H5N9V0_CITUN9.3e-14756.91Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_025730 PE=4 SV=1[more]
tr|A0A2P5E715|A0A2P5E715_9ROSA2.7e-13866.67Cdk-activating kinase assembly factor OS=Trema orientalis OX=63057 GN=TorRG33x02... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0046872metal ion binding
Vocabulary: INTERPRO
TermDefinition
IPR036855Znf_CCCH_sf
IPR017907Znf_RING_CS
IPR018957Znf_C3HC4_RING-type
IPR013083Znf_RING/FYVE/PHD
IPR000571Znf_CCCH
IPR001841Znf_RING
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13450-RACarg13450-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 206..263
e-value: 1.7E-7
score: 40.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 206..264
score: 12.371
IPR000571Zinc finger, CCCH-typeSMARTSM00356c3hfinal6coord: 29..55
e-value: 0.0013
score: 28.0
coord: 293..321
e-value: 0.36
score: 19.8
coord: 1..27
e-value: 5.7E-5
score: 32.5
coord: 137..163
e-value: 0.0033
score: 26.7
IPR000571Zinc finger, CCCH-typePFAMPF00642zf-CCCHcoord: 34..54
e-value: 1.2E-5
score: 24.9
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 29..56
score: 15.112
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 136..164
score: 12.436
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 293..322
score: 13.638
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 1..28
score: 13.685
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 192..298
e-value: 6.6E-10
score: 40.4
IPR018957Zinc finger, C3HC4 RING-typePFAMPF00097zf-C3HC4coord: 206..263
e-value: 2.1E-7
score: 30.6
NoneNo IPR availableGENE3DG3DSA:4.10.1000.10coord: 1..69
e-value: 3.3E-14
score: 54.6
NoneNo IPR availablePANTHERPTHR11224MAKORIN-RELATEDcoord: 1..324
NoneNo IPR availablePANTHERPTHR11224:SF51SUBFAMILY NOT NAMEDcoord: 1..324
NoneNo IPR availableCDDcd16521RING-HC_MKRNcoord: 206..264
e-value: 1.16764E-16
score: 73.505
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 194..270
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 230..239
IPR036855Zinc finger, CCCH-type superfamilySUPERFAMILYSSF90229CCCH zinc fingercoord: 3..26
IPR036855Zinc finger, CCCH-type superfamilySUPERFAMILYSSF90229CCCH zinc fingercoord: 32..53

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg13450Carg18680Silver-seed gourdcarcarB379