Carg13329 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg13329
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Rapid ALkalinization Factor
Location	Cucurbita_argyrosperma_scaffold_038 : 991029 .. 999100 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGATTCATGAAGCTCTGCCTATGTCTCCTCCTCATGGTTGTCGCCGCCGCGGCCGCCGCCCCACATTCCTCCGCCTTCTTCGGCGGATACACACCAGCAGAGTTGACGGAAGATGATCGACGGCTACTTTTCCAATACAAATACGACTGGAAGTTGTCTCGTAGAAAGCATTTAAACTACAAAGCGCATCGCGACAGCTCTTACTATGAACGCAGGATGCGAAGGAAGAAGGTGAAGCCTTATCCCCGTGGCTGCGCTGCCATCACCGGCTGCCCTCGAATCTCCGATTGAATGATGATCCGTTCGTGATATGTCCTTGAATGGGTTGGGCTGATTGTTTTCTTTTACAAATCCTGTGCTACGGATTGGAATTATCCAGTTCCTTAATGTTTAAATAAAATCATTGCAAATTCTAAATTAATTCCAATTTTATAAATTTTAATACAATTAGTCGAAGAAGTTACAAAGTTATGGAGCAACTTTAAAAGAACTTGCATGTCTTTTCACATCAACACAGAGAATTAAAAAACGTAAGTGCTTAAGGTTCTTCTATTCAAATGATCTATCATCAGTTAGTTCTTTCCACATATTTTGATCATAGGTCACATCAAATATATTTGATCGAATTTGCAAGAATTGACATGGAGCATAAATCGTTCATGTTTAGGAGAAGAGATTCAATTCTTTTTTCAAACTCTTGATCTCCAGTCGAATACATACACTCTAAATCTTACTTTTGATCGTGGGTTTGAAGCTTTTAACTTTGAATTATTTTTATCAACCAATTGAGCTATGTTCAAATTGAAAAACTTTTATTTTTAATATCGAAATTTTTGGTACGACAATGCATGTGAGACCTCTAATTGAGCTATGTTCAAATTGAAAAATTTTTATTTTTAATATCGAAATTTTTGGTACGACAATGCATGTGAGACCCTATGGAGGATTCAAAAGACGTATGGGCAGGCATCCAAGTCCATACACAAATTGGCTTCAAATAAGTAAGCCTTCCAAACAATCCTTTGATATTGCACATGCTTCACCTCTAGATTCATTATTGTCACACTTTTACTTGTTTTGCTATTCTACCAAAGGGAGCGAGCAATGAGCTTTTCTCCGTAATCAAAGCACTAAACTAACAATTCAATTAAAGTTCGTTCACAAGTTAGTAATTTATTCTACTAAGTCAAGTTTAATAGCTCACAATCTCTCCCGTAAACTTGATTGTGTTCGAGATTCTTAATTCCGAGCAACTCTTGCATCTCTGTAAGCTTAACTCTTGCTTTGGGCCTTGGTTAGAATATCTGCACGTTGCTCTCTATTGCATACGAAATCCACAACAATATGTCTTTTCTCGACACATTCAGGGATGAAGTGAAAGTGAGTGTCAATGTGTTTGCTGCGTCTGTGAAATACCAGATTCTTTATCAGTGCAACAGTAGATTTTTTGTCGACGTAGAGAGTTACCGACTTTAGTTCACTTCTAGTCAATTAGCTCAACAAATTTCTCAGCACAATGCTTGACACGATGCTACAGTTGTTGTCATGTACTCGGCCTCACAAGAAGATAGTGTTACAGTTCACTGCTTCTAAGATTGCCAAGAGATCAAATTTTCACTGAGATAAACTACCATTCCTACGGTGTCTTTTCTATTATCAATGTCTGTAGCTAAATCACTATCGGTAAAACAGACTAGTTCTTCAGGACTTCGCCCTTTTTGATATTTCAGCTTGTAGCTTGTGGTTTCCTTCACATAGTGGAGAATGTATTTAATCGCTTGATGATACATCGTATTTGGCTTTTTTACTCACCATTCCAACAACATATGAAAGGTCCAAACGAGTATCAGTTAAGTACCTCAGACTTTCGATGACGCACTGATACTTAGTGGAATTTATCAAGCTTCCTTCAGCACGTTTCTCAAGTTGTAACTTTGCTTCCATGGGATACTTGGTCGGGTTGCAAAATACCATGTGAAATTGTTGCAACAACTTCTTAGCAAGGCCAATTGCTTTAGCATGATGTAATCTTTCCTTTGGTCCACTTCAGTATCGAGGTAGCAAGTGAGTAACCCGAGATCAGTCATCTCAAATTCTTTCATCATTTGCTGCTTGAACTCTTTGAGACCTTCCATACTTGTACTAGTGACGATCAGGTCATCAACGTATACGTCAACTATGAGTGCTTGTTCTTGCGAGCACTTCATGAAGTTTAGATTCTTTAAGCTCTTGTCCAGATGTATGTTCCATGCCCTTGGTACTTGCCATAAACCGGAAAGAATCTTTGAAAACTTGTATACTTTGTGCTCTTCGATTATGGTGACGAACCCTTCTAGTTGGACAACATACATTTCTTCTTGGAGGTCATCAATTAGGAATGCTGATTTGACGTCCAAGTGATGGACCTTCCACCTGTATTGAGCTGTGAGGGCAAGAATCCACCTTACAGTGTTCAGTCTAGCCACATGCATGAAACCTCCTCAAAATCAACTCCTTGTCGTTGCACGAATCCTTTTGCCATGAGTCTTGCTTTATACGTGATGACATCCTTCGCTATTCTTCTTCAACTTAAACACCTACTTCAAATCGATGGGATTGTGTCTCGGCAATAAGTTAGTTAGTGCACACGTCTTTTTCTTCTCAGTTACTTCAAACTCTTTTTGCACGGTCTCCTACCACATTGTCTCAGTTGATGTCTCATAGTAAGTTTTCAGCTCCTCGACCATGAGCAACACTAGATCGTTGGGATCCAATTCTTATTCAAGTATATCGGCATAAATTTCAGTAAAAGAACGAAATTATGCCTGAGGTCCCAACATTTTTTTTCTTAAAATTTTCATTATCATTTAATACAACCCCGCTAGCGTCTTCCTTCATATCATGTCATTTTGTCATAATATTTCTCATAACATTTATCATAATTCTCATGCGTAGGTTGGGGTTGTATTTCATGCTAGTTTGTCATTTCGCATGTCTATCATATCATTCGTGTCAACATATATCATATCATAACATTTACATGTTCTCGGACATAACAATCCACATCATGCACATATCATAATAAACCCTAAATCATAACAATCACATAGTATATCCCAGACGTGTCTTCAACATATATTATCGTAATTATATCAATTCATTAAATATATCGTACCATAACATATATCATATCATTCTCATATTATATTACGATATGTATATATACATATATCGTTCCATATACATATCCTGGTCATATCGTTTCCTAAACTTATTCTAACAATATCATCTCACTAGTCTATCATAATCCTATCTTTTTTCATTTATTCAAACTGTCATAACCATAACGTTTTCTATACATAATCACATCGTATCGTTTTATCAATCTATCATAATCATAGTGTTTTCTATACATAACCTTATCGTATCGTCTTGTCAATCTATCATAACATATCGTTTTCTATACATAACCTTATCGTATCGTTTTATCAATCTATCATAACCATAGTATTTTCTATACATAACCTTATCGTATTGTTTTGCCAATCTATTATAACCATATCATTTCGATACATAACCTAACCATAACATTTTATGAACAAATCTTAGTCCTATCATTACATAACGTTTATCGCATCCTTCTCGTATCCTATCTCAAAATTATCATATAATACATCGTATCGTAAGTGTTATCATACAATTCACATATCATAACATATACACATCATATCATAATCATATTGATTCATAAACAATTCACACATATCAATATATATGACACATACAGAACCATGGGACACATGTCAACATAAAATATAATCATGTCGATAGTAAGTCCACTTACCTAATTAACTTAGGTTGATGTCACCAATTTATCTCTAATAAAAGTTTGATTTTTGTCCTTTGTACCCAAGTCAAACGGCCGTCCAAACTTTGATAACTCATTAACTTTTGTTCGGTTGACCAGGTGAACTAGTAAAAGTTTCCATTGTGACGTGCTAATTTTTTTTAATTTTTTTTTATTTATTATTTTATTTTAAGCTATTACACGTAACACTGACCCCAGCTGCCTCCAATGCATGCGCCCCAACCTAGCTGACCCCAGTTGTGGTTCGCATCTCGACTCCACTTAACTTGCAGTTGGCTCGATGACTTCAGCTTGACTTATTTGACGTATTCAGTTCATTTTGACCCACGATCATAATATACTGAAACAATCGAATAAAGTAGAAAGAATAACTTGATGGAAGAGATCCTTTGAACCGAACAACTTTAAAGACTATGTCTTCAATTCTCTTTGTTGTTGTAAATGGAACATACATCTTGTGAGTATTTACATTGGAGAAAACTTTAACATTTAAAGCTTCAAAAATACTAACAATTCTCTTTAATAAATCGCTAAACCGATTCCACAATTTGGGAACTACTAATAATTCTCTTTGAAGGGAATGAACTTTTTCATCCACCTATCCGGAGCTTTGGGAATTGTTTTGATTTTATTAAAGAATTAGAAAAAAGAAAAACAATAAATTCGCCCTAATTTGCATGACTGTTTGAAGTATTCAACCATGCAAGAGTTCTTCAACATTTCACGAGATCTTCCTTGTTATGACTCGAACACAAGGTCAAGGCTTGTTGGAGGCTTTTGAAAGAAAATCCCAAAGGGATTAGACAAATAGTTTCGAGAATTCTCTGGAGATGTAATTTCATTCTCCACGGAAAGTGGAAGAGATTGGTATGTCTTTGGTTCAACCTAAAGATCCTATTTATAGGGACTCTTAAAGAATTTTTGTCAAGTAACATACTGTGATATTTAATATATTAGAATATAATATCTTATACCAATCATATTTAGATATTGTAAGATATCCTAATGTATATTTAATTAATTTGAATATCATTCAAATTTCAAATATATTTTTATTAAATGTATCAACTACATTAATTGATTTAATATTTAATTGTAAATACAATAAATATATATTTTTTAATATATTAAATATATTAAATATTAATGGATCCTTTCAAATTAATATATTTTTCTCCATTTATATTTAACTTCTTTAATATATTAAATATTAATCTGAACTTTTCAAATTAAATATATTTTCTCTATTTATTTAATATATATAATATATTAAATATTAATTTCAAGTTTTTACATTTTTTTCAAATAAATTCTGAAAATTGAACGTTCAAATTAAATATATTTTGTCTATTTATTTAGTATATATAATATATTAAATATTAATTTGACATTTCAAGTTTTTACATTTTTTTCAAATAAATTCAGAAAATTAAACGTTTTAGTGTCAGTGTGAGTTAGCAAGGGGATCCTATAGATTATAAGTTCCAATGATATAAAATTAATTAATTAATCTATTTAATTAATCCATTTTCATTCATTAACTATAAGCCACTCCACTATGGATCTAGAGTTGCACTCTTGCGCACGGTAGGATAAGTTATGTCTTTTGATATAACCATTACAAGGAAGTCAATCCTTCACGAGTTGTTCATAATTGCAACTGGGTCAAAAATCTGTTTTACCCCTGTAATTACATCTTTCACCTTAATTTCACTGATTCTCTAATGAACAATTTGTTTATGATCCAATCATAAACTAAGTCCCTCTCGGGCCAATGAGAGGGTGGGGCCCATTGTTCAAGTCCCGAAATCAGTACTTAAAAGAACAACTCATCTACTTTTCTTATATGGAAATAAGTGAATTCCATCTACTGATATTATGTTCCTAATTCCCTATTTGGTGAAGTTTCCAAATGGTAGGCATATTGAATTGGCTAAAGAAGAGTCATTCTCACCCACTTAAATTAAAGGACATGTCCTCAAAGGTACGAGTTCATAACTCACTCAGAATTAAGATCGAGTCGCATATGATCATCATGTAAAATATTAATCTTCTCAATTAACGGATTAATAAAAAAGATTAAATATTTCTTGGTCCAGTCTTATACAAACTCATAGTATTGGATACCCCCGCTTACCGGTTTCCACATGAAAAATTTGGATCAAATCATTTATAACCATTACAAAGTGGGCCGTACCAACAGTGTTACCAGACACCCAACTTTATCCATATACTATAGACTCTTTAGGTTATTAATTGAACATAATCCTCATGTATGTCGACTATATACCGTTCAAGATTACATTAATAATCTTGGATTTTCATACCATAGATAACAATTAAATTACAAATAAAATAATAAAGATATCGTTAAAAACTAAATAATTATGTACTAGGTTTAGGTCATAAATCCCAACACTCCTAAAATTGCTCCCTAAGTAATTTCTTTTACTGAGTCAAAGTTATTGGTTCACAGTCTCGGGGTTAGAAGCTTTATTTTGTTCGAGATTCTTCACTCTCAGTAACTACTCCTGCATCTCTATAAACTCAACCCTTGCCACCATGAGAATATCCGCACGTTCCTCTTTGGTTCATAAGAACTCCACAACCATCTGTCTCTTCTCGTCACACTCACGAATGAAGTGGAAGCAAGTGTTAATTTGTTTGCTACGTCCGTAAAATACCAAATTCTTCTTCAATGCAATCACAAATTTGTCACCGACGTAGGGGGTTACCAGCTGTAGCTCACTTCTGGTCACTTCGCTCAACAAATTTCTCAGCCATAATGTTTGACACGATGTTGCAATTGCTGCCATGAACTCGACCTCATAGGAAGACTCGTAACTTCCTTCAACATCTTTCCCAAGTTGTAACTTTGCTTCCATGAGCCTACTAGGTAGACTCGTATACATACTCCATAGGCTTGACAAAGGGGTACCACATGCTCGCACTATTGGAAACCAACAAAAAGAATGGAGCACGTAGCATATCCTGGAGCAAGGGTTCGTACAAAATATATTAGAGAAGTATCTCTTGCTCATGATATACAACATGTACAAGGTCTTGCACTTTCAAATCATGGTACGTTGTTATGATGCACATCAAACTTACATACTTTTCATGACATAACCTTCCTATAAGACATACATACATGTTCATGACATATAATTCATTGTTCATCATACATGAATTATCAAGTATTGAATAAGACATAAAACAAACATGATATCATAACATACCTGATGAATTGACATACTAGAAGTACATGCATAAGAGGAAGATCATAAAAATTACATTATACAATTAATAACACATAGATAAGCCACCCAAAATGTGTTATTTATACAACATACAATTCATCCAACCTAACTCGAAAGTGTTCTAACGATAAGATCAATTATCTTGTTGGCCTAAACATAAGTCAATGAATGTTTTGAATCGATGTTTGTTTGATACTGCTCGACAAGGAATGGTTATGAAGTTGAGGTCGATAAGTTATTTGGGTTTGAAGTAGAGATTCACTAGGAGGTTTAGTTCCAAAACATTAGAGAATGAGATGGATTTGCATGATTAAGAAATGTTTAGAAGAATTAGTAATAACTACTGATATCGATCAAGTGTATTTTTCTTCTTAATGGCTCAATTGTAAGAACTTTAAAATTAAGCGTATTTGGCATGAAGCAATCGTATTTTGGGTGATCGTTTGAAAATTTTTCTATCGAACTGGCGGGTGAAGCTAAAAAAAACTCCGATCGATTTGTGAGGATAATTTTCACTCTCTAAAGTAGCCTAATCCATTACCATACTCGCACAGTTTTAAAATATTTGCCTAACTACGCCAAAACCAAAATAAAAACCCGAGTTTTTCCACGCATTTTCACAAACCAAAATGGGATTCATGAAGCTCTGCCTATGTCTCCTCCTCATGGTTGTCGCCGCCGCCCCTCCATATTCCTACGCCGCTCCTGACTACGCCTTCTTCGGCGGATATGCACCAGCACAGTTGACAGAAGATGGTCGACGGCTACTTTTCCAATACGGATACGACAGGACAAACAAGATGTATCGTAGAAGGTATTTAAGCTATGAGGCGCTTACGTTTAACAATATACCTTGCGGCCGTCGTGGCAGCTCTTACTATGACTGCAGGAAGCGAAGGAGGATCAACCCTTATCGCCGTGGCTGCACTGCCATCACCGGCTGCGCTCGATTCACCGATTGA

mRNA sequence

ATGGGATTCATGAAGCTCTGCCTATGTCTCCTCCTCATGGTTGTCGCCGCCGCGGCCGCCGCCCCACATTCCTCCGCCTTCTTCGGCGGATACACACCAGCAGAGTTGACGGAAGATGATCGACGGCTACTTTTCCAATACAAATACGACTGGAAGTTGTCTCGTAGAAAGCATTTAAACTACAAAGCGCATCGCGACAGCTCTTACTATGAACGCAGGATGCGAAGGAAGAAGCTCTGCCTATGTCTCCTCCTCATGGTTGTCGCCGCCGCCCCTCCATATTCCTACGCCGCTCCTGACTACGCCTTCTTCGGCGGATATGCACCAGCACAGTTGACAGAAGATGGTCGACGGCTACTTTTCCAATACGGATACGACAGGACAAACAAGATGTATCGTAGAAGGTATTTAAGCTATGAGGCGCTTACGTTTAACAATATACCTTGCGGCCGTCGTGGCAGCTCTTACTATGACTGCAGGAAGCGAAGGAGGATCAACCCTTATCGCCGTGGCTGCACTGCCATCACCGGCTGCGCTCGATTCACCGATTGA

Coding sequence (CDS)

ATGGGATTCATGAAGCTCTGCCTATGTCTCCTCCTCATGGTTGTCGCCGCCGCGGCCGCCGCCCCACATTCCTCCGCCTTCTTCGGCGGATACACACCAGCAGAGTTGACGGAAGATGATCGACGGCTACTTTTCCAATACAAATACGACTGGAAGTTGTCTCGTAGAAAGCATTTAAACTACAAAGCGCATCGCGACAGCTCTTACTATGAACGCAGGATGCGAAGGAAGAAGCTCTGCCTATGTCTCCTCCTCATGGTTGTCGCCGCCGCCCCTCCATATTCCTACGCCGCTCCTGACTACGCCTTCTTCGGCGGATATGCACCAGCACAGTTGACAGAAGATGGTCGACGGCTACTTTTCCAATACGGATACGACAGGACAAACAAGATGTATCGTAGAAGGTATTTAAGCTATGAGGCGCTTACGTTTAACAATATACCTTGCGGCCGTCGTGGCAGCTCTTACTATGACTGCAGGAAGCGAAGGAGGATCAACCCTTATCGCCGTGGCTGCACTGCCATCACCGGCTGCGCTCGATTCACCGATTGA

Protein sequence

MGFMKLCLCLLLMVVAAAAAAPHSSAFFGGYTPAELTEDDRRLLFQYKYDWKLSRRKHLNYKAHRDSSYYERRMRRKKLCLCLLLMVVAAAPPYSYAAPDYAFFGGYAPAQLTEDGRRLLFQYGYDRTNKMYRRRYLSYEALTFNNIPCGRRGSSYYDCRKRRRINPYRRGCTAITGCARFTD

Homology

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BLAST of Carg13329 vs. NCBI nr
Match: XP_022925244.1 (protein RALF-like 31 [Cucurbita moschata])

HSP 1 Score: 192.6 bits (488), Expect = 1.2e-45
Identity = 89/89 (100.00%), Postives = 89/89 (100.00%), Query Frame = 0


Query: 95  SYAAPDYAFFGGYAPAQLTEDGRRLLFQYGYDRTNKMYRRRYLSYEALTFNNIPCGRRGS 154
           SYAAPDYAFFGGYAPAQLTEDGRRLLFQYGYDRTNKMYRRRYLSYEALTFNNIPCGRRGS
Sbjct: 22  SYAAPDYAFFGGYAPAQLTEDGRRLLFQYGYDRTNKMYRRRYLSYEALTFNNIPCGRRGS 81

Query: 155 SYYDCRKRRRINPYRRGCTAITGCARFTD 184
           SYYDCRKRRRINPYRRGCTAITGCARFTD
Sbjct: 82  SYYDCRKRRRINPYRRGCTAITGCARFTD 110

[Close]

BLAST of Carg13329 vs. NCBI nr
Match: XP_022927737.1 (protein RALF-like 19 [Cucurbita moschata])

HSP 1 Score: 127.1 bits (318), Expect = 6.1e-26
Identity = 61/83 (73.49%), Postives = 68/83 (81.93%), Query Frame = 0


Query: 101 YAFFGGYAPAQLTEDGRRLLFQYGYDRTNKMYRRRYLSYEALTFNNIPCGRRGSSYYDCR 160
           Y+FFGG +   +TED RR LFQYG+   NK  R+RYLSY AL  NNIPCGRRG+SYYDC+
Sbjct: 40  YSFFGGDSSEAMTEDSRRQLFQYGF-VYNKEARKRYLSYYALRQNNIPCGRRGTSYYDCK 99

Query: 161 KRRRINPYRRGCTAITGCARFTD 184
           KRRRINPYRRGC AITGCARFTD
Sbjct: 100 KRRRINPYRRGCAAITGCARFTD 121

[Close]

BLAST of Carg13329 vs. NCBI nr
Match: XP_022927734.1 (protein RALF-like 4 [Cucurbita moschata])

HSP 1 Score: 127.1 bits (318), Expect = 6.1e-26
Identity = 60/83 (72.29%), Postives = 69/83 (83.13%), Query Frame = 0


Query: 101 YAFFGGYAPAQLTEDGRRLLFQYGYDRTNKMYRRRYLSYEALTFNNIPCGRRGSSYYDCR 160
           Y+FFGG +   +TED RR LFQYG+   NK  RRR+L+Y AL+ NNIPCGRRG+SYYDC+
Sbjct: 41  YSFFGGDSSEAMTEDSRRQLFQYGF-AYNKEARRRFLTYYALSKNNIPCGRRGTSYYDCK 100

Query: 161 KRRRINPYRRGCTAITGCARFTD 184
           KRRRINPYRRGC AITGCARFTD
Sbjct: 101 KRRRINPYRRGCAAITGCARFTD 122

[Close]

BLAST of Carg13329 vs. NCBI nr
Match: XP_022927736.1 (rapid alkalinization factor 23-like [Cucurbita moschata])

HSP 1 Score: 126.3 bits (316), Expect = 1.0e-25
Identity = 60/83 (72.29%), Postives = 69/83 (83.13%), Query Frame = 0


Query: 101 YAFFGGYAPAQLTEDGRRLLFQYGYDRTNKMYRRRYLSYEALTFNNIPCGRRGSSYYDCR 160
           Y+FFGG +   +TED RR LFQYG+   NK  R+R+LSY AL+ NNIPCGRRG+SYYDC+
Sbjct: 40  YSFFGGDSSEAMTEDSRRQLFQYGF-VYNKEARKRFLSYYALSKNNIPCGRRGTSYYDCK 99

Query: 161 KRRRINPYRRGCTAITGCARFTD 184
           KRRRINPYRRGC AITGCARFTD
Sbjct: 100 KRRRINPYRRGCAAITGCARFTD 121

[Close]

BLAST of Carg13329 vs. NCBI nr
Match: XP_022989014.1 (protein RALF-like 4 [Cucurbita maxima])

HSP 1 Score: 126.3 bits (316), Expect = 1.0e-25
Identity = 60/84 (71.43%), Postives = 68/84 (80.95%), Query Frame = 0


Query: 100 DYAFFGGYAPAQLTEDGRRLLFQYGYDRTNKMYRRRYLSYEALTFNNIPCGRRGSSYYDC 159
           +Y+FFGG +   +TED RR LFQYG+   NK  R+RYLSY AL  NNIPCGRRG+SYYDC
Sbjct: 39  EYSFFGGDSSEAMTEDSRRQLFQYGF-AYNKEARKRYLSYYALRQNNIPCGRRGTSYYDC 98

Query: 160 RKRRRINPYRRGCTAITGCARFTD 184
           +KRR INPYRRGC AITGCARFTD
Sbjct: 99  KKRRTINPYRRGCAAITGCARFTD 121

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BLAST of Carg13329 vs. TAIR10
Match: AT1G28270.1 (ralf-like 4)

HSP 1 Score: 81.3 bits (199), Expect = 7.0e-16
Identity = 34/48 (70.83%), Postives = 40/48 (83.33%), Query Frame = 0


Query: 134 RRYLSYEALTFNNIPCGRRGSSYYDCRKRRRINPYRRGCTAITGCARF 182
           RRY+ Y+AL  NN+PC RRG SYYDC+KRRR NPYRRGC+AIT C R+
Sbjct: 61  RRYIGYDALKKNNVPCSRRGRSYYDCKKRRRNNPYRRGCSAITHCYRY 108

[Close]

BLAST of Carg13329 vs. TAIR10
Match: AT2G33775.1 (ralf-like 19)

HSP 1 Score: 78.6 bits (192), Expect = 4.5e-15
Identity = 34/50 (68.00%), Postives = 39/50 (78.00%), Query Frame = 0


Query: 133 RRRYLSYEALTFNNIPCGRRGSSYYDCRKRRRINPYRRGCTAITGCARFT 183
           RR Y+SY AL  NN+PC RRG SYYDC+KR+R NPYRRGC+ IT C R T
Sbjct: 60  RRSYISYGALRKNNVPCSRRGRSYYDCKKRKRANPYRRGCSVITHCYRQT 109

[Close]

BLAST of Carg13329 vs. TAIR10
Match: AT3G16570.1 (rapid alkalinization factor 23)

HSP 1 Score: 69.3 bits (168), Expect = 2.8e-12
Identity = 30/47 (63.83%), Postives = 36/47 (76.60%), Query Frame = 0


Query: 134 RRYLSYEALTFNNIPCGRRGSSYYDCRKRRRINPYRRGCTAITGCAR 181
           RRY+SY AL  N IPC RRG+SYY+CR+  + NPY RGC+AIT C R
Sbjct: 91  RRYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 137

[Close]

BLAST of Carg13329 vs. TAIR10
Match: AT4G15800.1 (ralf-like 33)

HSP 1 Score: 65.9 bits (159), Expect = 3.0e-11
Identity = 27/46 (58.70%), Postives = 35/46 (76.09%), Query Frame = 0


Query: 135 RYLSYEALTFNNIPCGRRGSSYYDCRKRRRINPYRRGCTAITGCAR 181
           +Y+SY AL  N +PC RRG+SYY+CR+  + NPY RGC+AIT C R
Sbjct: 71  KYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 116

[Close]

BLAST of Carg13329 vs. TAIR10
Match: AT3G05490.1 (ralf-like 22)

HSP 1 Score: 63.2 bits (152), Expect = 2.0e-10
Identity = 24/48 (50.00%), Postives = 37/48 (77.08%), Query Frame = 0


Query: 133 RRRYLSYEALTFNNIPCGRRGSSYYDCRKRRRINPYRRGCTAITGCAR 181
           +++Y+SY A+  N++PC RRG+SYY+C++  + NPY RGC+ IT C R
Sbjct: 72  QKKYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRCRR 119

[Close]

BLAST of Carg13329 vs. Swiss-Prot
Match: sp|Q9FZA0|RLF4_ARATH (Protein RALF-like 4 OS=Arabidopsis thaliana OX=3702 GN=RALFL4 PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.3e-14
Identity = 34/48 (70.83%), Postives = 40/48 (83.33%), Query Frame = 0


Query: 134 RRYLSYEALTFNNIPCGRRGSSYYDCRKRRRINPYRRGCTAITGCARF 182
           RRY+ Y+AL  NN+PC RRG SYYDC+KRRR NPYRRGC+AIT C R+
Sbjct: 61  RRYIGYDALKKNNVPCSRRGRSYYDCKKRRRNNPYRRGCSAITHCYRY 108

[Close]

BLAST of Carg13329 vs. Swiss-Prot
Match: sp|Q6NME6|RLF19_ARATH (Protein RALF-like 19 OS=Arabidopsis thaliana OX=3702 GN=RALFL19 PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 8.2e-14
Identity = 34/50 (68.00%), Postives = 39/50 (78.00%), Query Frame = 0


Query: 133 RRRYLSYEALTFNNIPCGRRGSSYYDCRKRRRINPYRRGCTAITGCARFT 183
           RR Y+SY AL  NN+PC RRG SYYDC+KR+R NPYRRGC+ IT C R T
Sbjct: 60  RRSYISYGALRKNNVPCSRRGRSYYDCKKRKRANPYRRGCSVITHCYRQT 109

[Close]

BLAST of Carg13329 vs. Swiss-Prot
Match: sp|Q9LUS7|RLF23_ARATH (Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 5.0e-11
Identity = 30/47 (63.83%), Postives = 36/47 (76.60%), Query Frame = 0


Query: 134 RRYLSYEALTFNNIPCGRRGSSYYDCRKRRRINPYRRGCTAITGCAR 181
           RRY+SY AL  N IPC RRG+SYY+CR+  + NPY RGC+AIT C R
Sbjct: 91  RRYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 137

[Close]

BLAST of Carg13329 vs. Swiss-Prot
Match: sp|Q8L9P8|RLF33_ARATH (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 5.5e-10
Identity = 27/46 (58.70%), Postives = 35/46 (76.09%), Query Frame = 0


Query: 135 RYLSYEALTFNNIPCGRRGSSYYDCRKRRRINPYRRGCTAITGCAR 181
           +Y+SY AL  N +PC RRG+SYY+CR+  + NPY RGC+AIT C R
Sbjct: 71  KYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCRR 116

[Close]

BLAST of Carg13329 vs. Swiss-Prot
Match: sp|Q945T0|RALF_TOBAC (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.7e-09
Identity = 25/45 (55.56%), Postives = 35/45 (77.78%), Query Frame = 0


Query: 134 RRYLSYEALTFNNIPCGRRGSSYYDCRKRRRINPYRRGCTAITGC 179
           ++Y+SY AL  N++PC RRG+SYY+C+   + NPY RGC+AIT C
Sbjct: 69  KKYISYGALQKNSVPCSRRGASYYNCKPGAQANPYSRGCSAITRC 113

[Close]

BLAST of Carg13329 vs. TrEMBL
Match: tr|A0A1S4E1Z3|A0A1S4E1Z3_CUCME (protein RALF-like 4 OS=Cucumis melo OX=3656 GN=LOC107991607 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.6e-22
Identity = 55/91 (60.44%), Postives = 66/91 (72.53%), Query Frame = 0


Query: 95  SYAAPDYAFFGGYAPAQL--TEDGRRLLFQYGYDRTNKMYRRRYLSYEALTFNNIPCGRR 154
           ++  P+YAF+G  APA +   +D RRLLFQYG+    K  + +YL Y+AL  NN PC RR
Sbjct: 28  AFGNPEYAFWGNSAPATINDVDDSRRLLFQYGF--AYKYPKNKYLGYDALRKNNSPCRRR 87

Query: 155 GSSYYDCRKRRRINPYRRGCTAITGCARFTD 184
           G SYYDC KRR+ NPYRRGC AITGCARFTD
Sbjct: 88  GHSYYDCTKRRKANPYRRGCIAITGCARFTD 116

[Close]

BLAST of Carg13329 vs. TrEMBL
Match: tr|A0A0A0KCD7|A0A0A0KCD7_CUCSA (F3H9.8 protein OS=Cucumis sativus OX=3659 GN=Csa_6G199790 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.0e-21
Identity = 55/91 (60.44%), Postives = 64/91 (70.33%), Query Frame = 0


Query: 95  SYAAPDYAFFGGYAPAQL--TEDGRRLLFQYGYDRTNKMYRRRYLSYEALTFNNIPCGRR 154
           +Y  PDYAF+G   PA +   +D RRLLFQYG+    K  + +YL Y+AL  NN PC  R
Sbjct: 28  AYGNPDYAFWGTAEPATINDVDDSRRLLFQYGF--AYKYPKNKYLGYDALRKNNSPCRHR 87

Query: 155 GSSYYDCRKRRRINPYRRGCTAITGCARFTD 184
           G SYYDC KRR+ NPYRRGC AITGCARFTD
Sbjct: 88  GHSYYDCTKRRKANPYRRGCIAITGCARFTD 116

[Close]

BLAST of Carg13329 vs. TrEMBL
Match: tr|A0A1S3B2H7|A0A1S3B2H7_CUCME (protein RALF-like 19 OS=Cucumis melo OX=3656 GN=LOC103485256 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 7.4e-20
Identity = 53/89 (59.55%), Postives = 61/89 (68.54%), Query Frame = 0


Query: 95  SYAAPDYAFFGGYAPAQLTEDGRRLLFQYGYDRTNKMYRRRYLSYEALTFNNIPCGRRGS 154
           +YAAP+Y F          ED RRLLFQYG+    K  + +YL Y+AL  NN PC  RG 
Sbjct: 31  AYAAPNYDF------TTNNEDSRRLLFQYGF--AYKYPKNKYLGYDALRKNNSPCRHRGR 90

Query: 155 SYYDCRKRRRINPYRRGCTAITGCARFTD 184
           SYYDC+KRR+ NPYRRGC AITGCARFTD
Sbjct: 91  SYYDCKKRRKANPYRRGCIAITGCARFTD 111

[Close]

BLAST of Carg13329 vs. TrEMBL
Match: tr|A0A0A0KHU4|A0A0A0KHU4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G484570 PE=4 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.7e-19
Identity = 54/90 (60.00%), Postives = 61/90 (67.78%), Query Frame = 0


Query: 95  SYA-APDYAFFGGYAPAQLTEDGRRLLFQYGYDRTNKMYRRRYLSYEALTFNNIPCGRRG 154
           SYA  PDY F          ED RRLLFQYG+    K  + +YL Y+AL  NNIPC  RG
Sbjct: 31  SYADVPDYDF------TNSNEDSRRLLFQYGF--AYKYPKNKYLGYDALRKNNIPCRHRG 90

Query: 155 SSYYDCRKRRRINPYRRGCTAITGCARFTD 184
            SYYDC+KR++ NPYRRGC AITGCARFTD
Sbjct: 91  RSYYDCKKRKKANPYRRGCIAITGCARFTD 112

[Close]

BLAST of Carg13329 vs. TrEMBL
Match: tr|A0A2N9EP31|A0A2N9EP31_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4211 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.7e-13
Identity = 35/51 (68.63%), Postives = 43/51 (84.31%), Query Frame = 0


Query: 133 RRRYLSYEALTFNNIPCGRRGSSYYDCRKRRRINPYRRGCTAITGCARFTD 184
           RR+Y+SY AL  N+IPCGRRG+SYY+C++R+R NPYRRGC  IT CAR TD
Sbjct: 67  RRKYISYGALRANSIPCGRRGNSYYNCQRRQRANPYRRGCNIITHCARITD 117

The following BLAST results are available for this feature:

BLAST of Carg13329 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022925244.1	1.2e-45	100.00	protein RALF-like 31 [Cucurbita moschata]	[more]
XP_022927737.1	6.1e-26	73.49	protein RALF-like 19 [Cucurbita moschata]	[more]
XP_022927734.1	6.1e-26	72.29	protein RALF-like 4 [Cucurbita moschata]	[more]
XP_022927736.1	1.0e-25	72.29	rapid alkalinization factor 23-like [Cucurbita moschata]	[more]
XP_022989014.1	1.0e-25	71.43	protein RALF-like 4 [Cucurbita maxima]	[more]

BLAST of Carg13329 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G28270.1	7.0e-16	70.83	ralf-like 4	[more]
AT2G33775.1	4.5e-15	68.00	ralf-like 19	[more]
AT3G16570.1	2.8e-12	63.83	rapid alkalinization factor 23	[more]
AT4G15800.1	3.0e-11	58.70	ralf-like 33	[more]
AT3G05490.1	2.0e-10	50.00	ralf-like 22	[more]

BLAST of Carg13329 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9FZA0\|RLF4_ARATH	1.3e-14	70.83	Protein RALF-like 4 OS=Arabidopsis thaliana OX=3702 GN=RALFL4 PE=3 SV=1	[more]
sp\|Q6NME6\|RLF19_ARATH	8.2e-14	68.00	Protein RALF-like 19 OS=Arabidopsis thaliana OX=3702 GN=RALFL19 PE=3 SV=1	[more]
sp\|Q9LUS7\|RLF23_ARATH	5.0e-11	63.83	Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV...	[more]
sp\|Q8L9P8\|RLF33_ARATH	5.5e-10	58.70	Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1	[more]
sp\|Q945T0\|RALF_TOBAC	2.7e-09	55.56	Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1	[more]

BLAST of Carg13329 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S4E1Z3\|A0A1S4E1Z3_CUCME	3.6e-22	60.44	protein RALF-like 4 OS=Cucumis melo OX=3656 GN=LOC107991607 PE=4 SV=1	[more]
tr\|A0A0A0KCD7\|A0A0A0KCD7_CUCSA	1.0e-21	60.44	F3H9.8 protein OS=Cucumis sativus OX=3659 GN=Csa_6G199790 PE=4 SV=1	[more]
tr\|A0A1S3B2H7\|A0A1S3B2H7_CUCME	7.4e-20	59.55	protein RALF-like 19 OS=Cucumis melo OX=3656 GN=LOC103485256 PE=4 SV=1	[more]
tr\|A0A0A0KHU4\|A0A0A0KHU4_CUCSA	1.7e-19	60.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G484570 PE=4 SV=1	[more]
tr\|A0A2N9EP31\|A0A2N9EP31_FAGSY	6.7e-13	68.63	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4211 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13329-RA	Carg13329-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 132..180 e-value: 4.6E-18 score: 65.7
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 79..182
None	No IPR available	PANTHER	PTHR33136:SF16	PROTEIN RALF-LIKE 19-RELATED	coord: 79..182

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg13329	CmoCh07G011650	Cucurbita moschata (Rifu)	carcmoB0795
Carg13329	Cp4.1LG19g02330	Cucurbita pepo (Zucchini)	carcpeB0830

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg13329	Cucumber (Gy14) v2	carcgybB0583
Carg13329	Cucurbita maxima (Rimu)	carcmaB0812
Carg13329	Cucurbita maxima (Rimu)	carcmaB0818
Carg13329	Cucurbita moschata (Rifu)	carcmoB0789
Carg13329	Cucurbita pepo (Zucchini)	carcpeB0827
Carg13329	Wild cucumber (PI 183967)	carcpiB0615
Carg13329	Cucumber (Chinese Long) v2	carcuB0609
Carg13329	Cucumber (Chinese Long) v3	carcucB0616
Carg13329	Bottle gourd (USVL1VR-Ls)	carlsiB527
Carg13329	Melon (DHL92) v3.5.1	carmeB0585
Carg13329	Melon (DHL92) v3.6.1	carmedB0592
Carg13329	Watermelon (Charleston Gray)	carwcgB0566
Carg13329	Watermelon (97103) v1	carwmB0577
Carg13329	Watermelon (97103) v2	carwmbB0606
Carg13329	Wax gourd	carwgoB0694