Carg13174 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg13174
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	homogentisate 1,2-dioxygenase
Location	Cucurbita_argyrosperma_scaffold_101 : 768965 .. 772747 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAGCTTGAGCTTCTTGAACACGTCATGACGTGATTTCGATCGTGAATCTAGTTGCGTGGGGGAATCACCGATCATTGGAACTGCCGCGTGTTCTCCGGTGCCGTTTTCCGGTTTCAATAGTCTCCACAATCGATCATCATCTTCATCTGCTTTTTATTCTTCTTCATTTTCAATGGCTGCTCAATCGGTCGGCGAAATGGACGGCGGAAATTTCCCTTCCGACCTCGCCTACCAGTCCGGCTTCAACAATCATTTCTCGTCGGAGGCGATTCCCGGTGCTCTTCCTCAATTGCAAAACAGTCCTCTGATATGCCCTTACGGCCTCTATGCCGAGCAGATCTCTGGCACTTCCTTCACATCGCCTCGCAAAGTCAACATGTGCAGGTATTCTTTTGTCTTCTCGTGTTTTCGGTGTGGTTTTTCTGGTTTTGGTGTCTTTGTTGTGTACGCATTTCGGTTTTTGGTGCGCAGTTGGCTGTATAGGATTAAGCCGTCGGTCACGCATGAACCGTTTAGGCCTCGTTTGCCTAAAAACGAGAAGTTGATCAGTGAATTTAATGCGTCAAATTGTTCATCGACTCCGACTCAGCTAAGGTGGAGACCGGCGGAGGTTCCTGATTCACCGCTGGATTTCGTTGATGGTCTGTACACTGTTTGTGGAGCCGGCAGTTCGTTTCTTCGGCATGGTTTTGCTATTCACATGTAAGTATGTAATCAGTATTCCTTCTACGGTTGATTTGAATCTTTTTCTAACAATCAGTAAATTTTTAATGGGAAGGTATTATTTGTCGGATTTATCGGTGGGATATTTTGTCAGAGCAGAAAATATGATCTCTTAAGTTCGACTCTCTTTTTCCCACCATGGGGGTCGTTCGTTACCAACACGAAACAGTACAAATTTTAAACATTGTGTTTACGAGTATTGATCCCCCCCCCCAATAAATAATATTCTCAATACCTTTTTCTGGGATTTGGTGGGGAAGAAAGCAATAAAGATACGCTTTCACACTGGTCATTGGTTGAATGCTATGACTGAAACAGTATTGATCATTTGTATAGGTACACAGCCAATAAGTCAATGGAGAACTGTGCGTTCTGTAATGCTGACGGCGACTTCTTGATAGTCCCTCAGAGTGGAAGTGAGTTATTGTTGGTAGTTACTTTCATTGAAAATTATGAACGTAGTGGGCTTCAATCTGAGTATTTTTATTCGGAAAATCTAGTTAGTTTTAGATTCATTGCTCGACATCTTGTGCGATCTGTAAAGCTGATTCCTTATAGTGAAAGTGATGTAAAGCTGATTCCAATTCTAAGTTTTCAACTTTTGGCTACATACATAATCCTCAACTGTCTTTGCATTATCTTGTCATTGAGTTATGGAAGTCTCTGCTGTATATTGAATCTGAAGGAAACTATATTCCTGTCTTAAAAGTTCTAAGGAAACAGGATCAAACTCTTATAGAATATTCTTATTCCATAGGGCTGTGGATTATTACCGAGTGTGGAAGACTGGAAGTTTCTCCTGGTGAAATAGTGGTTTTACCTCAAGGTTTTCGCTTTGTTGTTTATCTGCCTGATGGTCCATCACGTGGCTACGTAGCTGAGATTTTTGGCAGTCATTTTCAGCTTCCTGATCTTGGACCAATAGGTATCTAACCTCTTATTATCTTCTCGACAGCCATTTATTTAGGTTTTGACCACATTCTTTTCCCTTTTTCCCCAACATTGGGAAATGGTTTTCTGAGTTTTGCATTTTCAACTAATGTAATATTGAGGGCTAAGTCCATTGACATGTAGGTGCTAATGGTCTTGCTGCACCACGGGATTTTCTTGCCCCTGTAGCCTGGTTTGAAAATATTTCTCGTCCTGGTTACACAATTGTGCAAAAATATGGTGGGGAATTGTTTACTGCGATACAGGACTTTTCTCCCTTTAATGTAGTTGCCTGGCATGGTAATTATGTTCCCTATAAGGTGGGTGTTGCGTATGCCATTGAGTATAGCTTAACTTTGGTTTCTTTTTGTGGAATTAAAAATAAGTTTGTCTATACTTTCTTTCAGGCTTTTTGCTATTTATGTCTTATTATAGACTAATGTTTCTTATCTTTGCACGTGTGTGCCAATTCCACCTCAATATAACTGAAAGTTGATCCTTCTCCTTGCAGTACGATCTTAGTAAGTTCTGCCCTTACAATACTGTGTTATTTGATCACAGTGATCCATCAATAAATACAGGTACATACATATAATGCATTAATTTATTTCACATTAAATACACTACTTTAAAATCCTTGACATTTCATTGAATTTCAAGTGTTTTATTCGCCTATCTGAATTATCATGCTGCTGCCCTGTTGTAGTATTAACGGCACCAACTGATAAACCCGGAGTAGCGTTACTCGACTTTGTCATTTTCCCCCCAAGATGGCTCGTCTCTGAACACACATTCCGTCCTCCATATTACCATCGCAACTGCATGAGTGAATTTATGGGTCTCATATATGGAGGCTACGAGGTAATTTTCATCGTCTCGTTTTTAGATAAAGAGACTGAATGAACCAAAATATTTAGGGAAGGAGGAAGCGAAGTTGCTTCTTTTATTATTACTCTACTTGCCTTATAAGGTCGCAAAAGCCTCTCTTTGCCTGTTACCTTAATGATAATGTTGTTTCTAGGCAAAAAATTGGTTCTGACTTCTCCCCCTTTACTTCGACAGGGTTCTAGTTTACATTTCAAGTGGATTGATTTTATTCCAGTAATGTATTTTTTTCCCTAGATCTTTAGAATTTCTGATTCATCTTTCAGGCAAAAGCTGATGGGTTCCTTCCTGGGGGTGCCAGCCTTCATAATTGCATGACTCCCCATGGCCCTGATACTAAAACATACGAGGTATGCATTGCTTCTTATAAGTTCCATTATTCATTTAATTTCCGCCACATTCAAACTTTCTTCTTCACAAGATTCTCTGGTGGCATTTCGTAGTTGTAAGGTATTTAATTCTAGAGAAGTAGTCATTGTGGCTTGACCGGATTCCCTCTAAAACCTCTAAGCCCTTGAAGATTACATGATCCTTATTGCCCACTATGTCAACTCATGGAAGTTTACTTGGAAAGCTACAAATGGATCTTCTCATTCTTCCAACAGTGTATACTTTTAGTTTAAACCATTCCTTTTATGATTCAACGAAAAGTCAGTAAGCATTCTCTTGATTTAGGCCACTATTGCCCGAGGAAACGAGGCTGGACCTTATCGAATCACCGACACAATGGCATTTATGTTTGAATCAAGTCTGATCCCTCGCGTATGCTCTTGGGCTCTGGAGTCTCCATGCATGGATCATGACTACTACCAATGCTGGATAGGATTGAAATCTCATTTCACAAGTGAAGGAACAACTAGAGACATGGATCCACAGAAGGTAAAAACTGACGTTGAAAATGGAAGACAGACTGTGTAGATATAATATTGGATGAACATCATGGATTTCATGAAAAAGTTTCCATCCAAAGTGTACATATAAGAATAAAATTATGAATCTTATAAAAAATAATAATAAATTATGACAAAATGATGAATCTTCTTGCTGTAACTTCTTTACTCTGGTAATGCCTACATTTTGCCTACATTTGTTAATCTTTCAATAGTAAATATTTGATCTGACCTTAGCTCAAGGGAGATCCCCCTTGCGCCTTGATTCCGTGCCTCTTTCTTTGGTATCGTCAAATGATTCTATTATGGTCGGTAAAAGTCTACTACCCATATGAGTTTTTGTTTTTTCT

mRNA sequence

ATGGTGAGCTTGAGCTTCTTGAACACTTGCGTGGGGGAATCACCGATCATTGGAACTGCCGCGTGTTCTCCGGTGCCGTTTTCCGGTTTCAATAGTCTCCACAATCGATCATCATCTTCATCTGCTTTTTATTCTTCTTCATTTTCAATGGCTGCTCAATCGGTCGGCGAAATGGACGGCGGAAATTTCCCTTCCGACCTCGCCTACCAGTCCGGCTTCAACAATCATTTCTCGTCGGAGGCGATTCCCGGTGCTCTTCCTCAATTGCAAAACAGTCCTCTGATATGCCCTTACGGCCTCTATGCCGAGCAGATCTCTGGCACTTCCTTCACATCGCCTCGCAAAGTCAACATGTGCAGTTGGCTGTATAGGATTAAGCCGTCGGTCACGCATGAACCGTTTAGGCCTCGTTTGCCTAAAAACGAGAAGTTGATCAGTGAATTTAATGCGTCAAATTGTTCATCGACTCCGACTCAGCTAAGGTGGAGACCGGCGGAGGTTCCTGATTCACCGCTGGATTTCGTTGATGGTCTGTACACTGTTTGTGGAGCCGGCAGTTCGTTTCTTCGGCATGGTTTTGCTATTCACATGTACACAGCCAATAAGTCAATGGAGAACTGTGCGTTCTGTAATGCTGACGGCGACTTCTTGATAGTCCCTCAGAGTGGAAGTGAGTTATTGTTGGTAGTTACTTTCATTGAAAATTATGAACGTAGTGGGCTTCAATCTGAGTATTTTTATTCGGAAAATCTATCTCTGCTGTATATTGAATCTGAAGGAAACTATATTCCTGTCTTAAAAGTTCTAAGGAAACAGGATCAAACTCTTATAGAATATTCTTATTCCATAGGGCTGTGGATTATTACCGAGTGTGGAAGACTGGAAGTTTCTCCTGGTGAAATAGTGGTTTTACCTCAAGGTTTTCGCTTTGTTGTTTATCTGCCTGATGGTCCATCACGTGGCTACGTAGCTGAGATTTTTGGCAGTCATTTTCAGCTTCCTGATCTTGGACCAATAGGTGCTAATGGTCTTGCTGCACCACGGGATTTTCTTGCCCCTGTAGCCTGGTTTGAAAATATTTCTCGTCCTGGTTACACAATTGTGCAAAAATATGGTGGGGAATTGTTTACTGCGATACAGGACTTTTCTCCCTTTAATGTAGTTGCCTGGCATGGTAATTATGTTCCCTATAAGTACGATCTTAGTAAGTTCTGCCCTTACAATACTGTGTTATTTGATCACAGTGATCCATCAATAAATACAGTATTAACGGCACCAACTGATAAACCCGGAGTAGCGTTACTCGACTTTGTCATTTTCCCCCCAAGATGGCTCGTCTCTGAACACACATTCCGTCCTCCATATTACCATCGCAACTGCATGAGTGAATTTATGGGTCTCATATATGGAGGCTACGAGGCAAAAGCTGATGGGTTCCTTCCTGGGGGTGCCAGCCTTCATAATTGCATGACTCCCCATGGCCCTGATACTAAAACATACGAGGCCACTATTGCCCGAGGAAACGAGGCTGGACCTTATCGAATCACCGACACAATGGCATTTATGTTTGAATCAAGTCTGATCCCTCGCGTATGCTCTTGGGCTCTGGAGTCTCCATGCATGGATCATGACTACTACCAATGCTGGATAGGATTGAAATCTCATTTCACAAGTGAAGGAACAACTAGAGACATGGATCCACAGAAGGTAAAAACTGACGTTGAAAATGGAAGACAGACTGTGTAGATATAATATTGGATGAACATCATGGATTTCATGAAAAAGTTTCCATCCAAAGTGTACATATAAGAATAAAATTATGAATCTTATAAAAAATAATAATAAATTATGACAAAATGATGAATCTTCTTGCTGTAACTTCTTTACTCTGGTAATGCCTACATTTTGCCTACATTTGTTAATCTTTCAATAGTAAATATTTGATCTGACCTTAGCTCAAGGGAGATCCCCCTTGCGCCTTGATTCCGTGCCTCTTTCTTTGGTATCGTCAAATGATTCTATTATGGTCGGTAAAAGTCTACTACCCATATGAGTTTTTGTTTTTTCT

Coding sequence (CDS)

ATGGTGAGCTTGAGCTTCTTGAACACTTGCGTGGGGGAATCACCGATCATTGGAACTGCCGCGTGTTCTCCGGTGCCGTTTTCCGGTTTCAATAGTCTCCACAATCGATCATCATCTTCATCTGCTTTTTATTCTTCTTCATTTTCAATGGCTGCTCAATCGGTCGGCGAAATGGACGGCGGAAATTTCCCTTCCGACCTCGCCTACCAGTCCGGCTTCAACAATCATTTCTCGTCGGAGGCGATTCCCGGTGCTCTTCCTCAATTGCAAAACAGTCCTCTGATATGCCCTTACGGCCTCTATGCCGAGCAGATCTCTGGCACTTCCTTCACATCGCCTCGCAAAGTCAACATGTGCAGTTGGCTGTATAGGATTAAGCCGTCGGTCACGCATGAACCGTTTAGGCCTCGTTTGCCTAAAAACGAGAAGTTGATCAGTGAATTTAATGCGTCAAATTGTTCATCGACTCCGACTCAGCTAAGGTGGAGACCGGCGGAGGTTCCTGATTCACCGCTGGATTTCGTTGATGGTCTGTACACTGTTTGTGGAGCCGGCAGTTCGTTTCTTCGGCATGGTTTTGCTATTCACATGTACACAGCCAATAAGTCAATGGAGAACTGTGCGTTCTGTAATGCTGACGGCGACTTCTTGATAGTCCCTCAGAGTGGAAGTGAGTTATTGTTGGTAGTTACTTTCATTGAAAATTATGAACGTAGTGGGCTTCAATCTGAGTATTTTTATTCGGAAAATCTATCTCTGCTGTATATTGAATCTGAAGGAAACTATATTCCTGTCTTAAAAGTTCTAAGGAAACAGGATCAAACTCTTATAGAATATTCTTATTCCATAGGGCTGTGGATTATTACCGAGTGTGGAAGACTGGAAGTTTCTCCTGGTGAAATAGTGGTTTTACCTCAAGGTTTTCGCTTTGTTGTTTATCTGCCTGATGGTCCATCACGTGGCTACGTAGCTGAGATTTTTGGCAGTCATTTTCAGCTTCCTGATCTTGGACCAATAGGTGCTAATGGTCTTGCTGCACCACGGGATTTTCTTGCCCCTGTAGCCTGGTTTGAAAATATTTCTCGTCCTGGTTACACAATTGTGCAAAAATATGGTGGGGAATTGTTTACTGCGATACAGGACTTTTCTCCCTTTAATGTAGTTGCCTGGCATGGTAATTATGTTCCCTATAAGTACGATCTTAGTAAGTTCTGCCCTTACAATACTGTGTTATTTGATCACAGTGATCCATCAATAAATACAGTATTAACGGCACCAACTGATAAACCCGGAGTAGCGTTACTCGACTTTGTCATTTTCCCCCCAAGATGGCTCGTCTCTGAACACACATTCCGTCCTCCATATTACCATCGCAACTGCATGAGTGAATTTATGGGTCTCATATATGGAGGCTACGAGGCAAAAGCTGATGGGTTCCTTCCTGGGGGTGCCAGCCTTCATAATTGCATGACTCCCCATGGCCCTGATACTAAAACATACGAGGCCACTATTGCCCGAGGAAACGAGGCTGGACCTTATCGAATCACCGACACAATGGCATTTATGTTTGAATCAAGTCTGATCCCTCGCGTATGCTCTTGGGCTCTGGAGTCTCCATGCATGGATCATGACTACTACCAATGCTGGATAGGATTGAAATCTCATTTCACAAGTGAAGGAACAACTAGAGACATGGATCCACAGAAGGTAAAAACTGACGTTGAAAATGGAAGACAGACTGTGTAG

Protein sequence

MVSLSFLNTCVGESPIIGTAACSPVPFSGFNSLHNRSSSSSAFYSSSFSMAAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTSFTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPDSPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLVVTFIENYERSGLQSEYFYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIITECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIPRVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPQKVKTDVENGRQTV

Homology

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BLAST of Carg13174 vs. NCBI nr
Match: XP_022980282.1 (homogentisate 1,2-dioxygenase [Cucurbita maxima])

HSP 1 Score: 1052.4 bits (2720), Expect = 5.8e-304
Identity = 512/588 (87.07%), Postives = 515/588 (87.59%), Query Frame = 0


Query: 4   LSFLNT----------CVGESPIIGTAACSPVPFSGFNSLHNRSSSSSAFYSSSFSMAAQ 63
           +SFLNT          CVGESPIIGTAACSPVPFSGFNSLHNRSSSSSAFYSSS SMAAQ
Sbjct: 2   VSFLNTSLRDFDRESSCVGESPIIGTAACSPVPFSGFNSLHNRSSSSSAFYSSSVSMAAQ 61

Query: 64  SVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTSFTSP 123
           SVGEMDG NFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTSFTSP
Sbjct: 62  SVGEMDGENFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTSFTSP 121

Query: 124 RKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPDSPLD 183
           RKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPA+VPDSPLD
Sbjct: 122 RKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPADVPDSPLD 181

Query: 184 FVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLVVTFI 243
           FVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSG          
Sbjct: 182 FVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGR--------- 241

Query: 244 ENYERSGLQSEYFYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIITECGR 303
                                                              LWIITECGR
Sbjct: 242 ---------------------------------------------------LWIITECGR 301

Query: 304 LEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAP 363
           LEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAP
Sbjct: 302 LEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAP 361

Query: 364 VAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFD 423
           VAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFD
Sbjct: 362 VAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFD 421

Query: 424 HSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIYGGYE 483
           HSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIYGGYE
Sbjct: 422 HSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIYGGYE 481

Query: 484 AKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIPRVCS 543
           AKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIPRVCS
Sbjct: 482 AKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIPRVCS 529

Query: 544 WALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPQKVKTDVENGRQTV 582
           WALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDP+KVKTDVENGRQTV
Sbjct: 542 WALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPRKVKTDVENGRQTV 529

[Close]

BLAST of Carg13174 vs. NCBI nr
Match: XP_022942212.1 (homogentisate 1,2-dioxygenase [Cucurbita moschata])

HSP 1 Score: 981.9 bits (2537), Expect = 9.6e-283
Identity = 469/532 (88.16%), Postives = 470/532 (88.35%), Query Frame = 0


Query: 50  MAAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS 109
           MAAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS
Sbjct: 1   MAAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS 60

Query: 110 FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPD 169
           FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPD
Sbjct: 61  FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPD 120

Query: 170 SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLV 229
           SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSG      
Sbjct: 121 SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGR----- 180

Query: 230 VTFIENYERSGLQSEYFYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIIT 289
                                                                  LWIIT
Sbjct: 181 -------------------------------------------------------LWIIT 240

Query: 290 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 349
           ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD
Sbjct: 241 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 300

Query: 350 FLAPVAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 409
           FLAPVAWFEN+SRPGYTIVQKYGGE FTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT
Sbjct: 301 FLAPVAWFENLSRPGYTIVQKYGGEFFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 360

Query: 410 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY 469
           VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 361 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY 420

Query: 470 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 529
           GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP
Sbjct: 421 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 472

Query: 530 RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPQKVKTDVENGRQTV 582
           RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPQKVKTDVENGRQTV
Sbjct: 481 RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPQKVKTDVENGRQTV 472

[Close]

BLAST of Carg13174 vs. NCBI nr
Match: XP_023550715.1 (homogentisate 1,2-dioxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 978.0 bits (2527), Expect = 1.4e-281
Identity = 469/532 (88.16%), Postives = 470/532 (88.35%), Query Frame = 0


Query: 50  MAAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS 109
           MAAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS
Sbjct: 1   MAAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS 60

Query: 110 FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPD 169
           FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPA+VPD
Sbjct: 61  FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPADVPD 120

Query: 170 SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLV 229
           SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSG      
Sbjct: 121 SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGR----- 180

Query: 230 VTFIENYERSGLQSEYFYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIIT 289
                                                                  L IIT
Sbjct: 181 -------------------------------------------------------LLIIT 240

Query: 290 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 349
           ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD
Sbjct: 241 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 300

Query: 350 FLAPVAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 409
           FLAPVAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT
Sbjct: 301 FLAPVAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 360

Query: 410 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY 469
           VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 361 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY 420

Query: 470 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 529
           GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP
Sbjct: 421 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 472

Query: 530 RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPQKVKTDVENGRQTV 582
           RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPQKVKTDVENGRQTV
Sbjct: 481 RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPQKVKTDVENGRQTV 472

[Close]

BLAST of Carg13174 vs. NCBI nr
Match: XP_004137214.1 (PREDICTED: homogentisate 1,2-dioxygenase [Cucumis sativus] >KGN53602.1 hypothetical protein Csa_4G088750 [Cucumis sativus])

HSP 1 Score: 899.8 bits (2324), Expect = 4.8e-258
Identity = 428/530 (80.75%), Postives = 448/530 (84.53%), Query Frame = 0


Query: 50  MAAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS 109
           MAAQSVGE DG +FPSDL Y SGFNNHFSSEAIPGALPQ QNSPLICP+GLYAEQISGTS
Sbjct: 1   MAAQSVGETDGTDFPSDLPYLSGFNNHFSSEAIPGALPQSQNSPLICPFGLYAEQISGTS 60

Query: 110 FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPD 169
           FTSPRK N+CSWLYRIKPSVTHEPFR RLPKNEKLISEFNASNCSSTPTQLRW+PA+ PD
Sbjct: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCSSTPTQLRWKPADFPD 120

Query: 170 SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLV 229
           SP+DFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSG      
Sbjct: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGK----- 180

Query: 230 VTFIENYERSGLQSEYFYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIIT 289
                                                                  LWIIT
Sbjct: 181 -------------------------------------------------------LWIIT 240

Query: 290 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 349
           ECGRLEVSPGE+VVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD
Sbjct: 241 ECGRLEVSPGEVVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 300

Query: 350 FLAPVAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 409
           FLAPVAWFEN  RPGYTI+QK+GGELFTAIQDFSPFNVVAWHGNYVPYKYDL KFCPYNT
Sbjct: 301 FLAPVAWFENSPRPGYTIIQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLCKFCPYNT 360

Query: 410 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY 469
           VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLV+EHTFRPPYYHRNCMSEFMGLIY
Sbjct: 361 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 420

Query: 470 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 529
           GGYEAKADGF+PGGASLH+CMTPHGPDTKTYEATIARGN+AGP++I+ TMAFMFESSLIP
Sbjct: 421 GGYEAKADGFVPGGASLHSCMTPHGPDTKTYEATIARGNDAGPHKISGTMAFMFESSLIP 469

Query: 530 RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPQKVKTDVENGRQ 580
           RVCSWALESP +DHDYYQCWIGLKSHF +E    D DPQKV+ + ENGRQ
Sbjct: 481 RVCSWALESPFIDHDYYQCWIGLKSHFKNE-AIGDTDPQKVRIESENGRQ 469

[Close]

BLAST of Carg13174 vs. NCBI nr
Match: XP_022142871.1 (homogentisate 1,2-dioxygenase [Momordica charantia])

HSP 1 Score: 896.3 bits (2315), Expect = 5.3e-257
Identity = 427/530 (80.57%), Postives = 449/530 (84.72%), Query Frame = 0


Query: 50  MAAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS 109
           MAAQSV E DG +FPSDLAYQSGFNNHFSSEAIPGALP+ QNSPLICPYGLYAEQISGTS
Sbjct: 1   MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS 60

Query: 110 FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPD 169
           FTSPRK N+ SWLYRIKPSVTHEPF+PRLPKN+KLISEFN S+CSSTPTQLRW+PA+ PD
Sbjct: 61  FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD 120

Query: 170 SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLV 229
           SP+DFVDGLYTVCGAGSSFLRHGFA+HMYTANKSMENCAFCNADGDFLIVPQSG      
Sbjct: 121 SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGR----- 180

Query: 230 VTFIENYERSGLQSEYFYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIIT 289
                                                                  LWIIT
Sbjct: 181 -------------------------------------------------------LWIIT 240

Query: 290 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 349
           ECGRLEVSPGEIVVLPQGFRFVV+LPDGPSRGY+AEIFGSHFQLPDLGPIGANGLAAP+D
Sbjct: 241 ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD 300

Query: 350 FLAPVAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 409
           FLAPVAWFE   R GYTIVQK+GGELFTAIQDFSPFNVVAWHG+YVPYKYDLSKFCPYNT
Sbjct: 301 FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT 360

Query: 410 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY 469
           VLFDH DPSINTVLTAPTDKPGVALLDFVIFPPRWLV+EHTFRPPYYHRNCMSEFMGLIY
Sbjct: 361 VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 420

Query: 470 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 529
           GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP
Sbjct: 421 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 469

Query: 530 RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPQKVKTDVENGRQ 580
           RVC+WALESP MD DYY+CWIGLKSHFT++  TRD DP+KVKT+  NGRQ
Sbjct: 481 RVCAWALESPFMDRDYYRCWIGLKSHFTNK-ETRDTDPKKVKTEAGNGRQ 469

[Close]

BLAST of Carg13174 vs. TAIR10
Match: AT5G54080.1 (homogentisate 1,2-dioxygenase)

HSP 1 Score: 758.1 bits (1956), Expect = 4.1e-219
Identity = 355/492 (72.15%), Postives = 389/492 (79.07%), Query Frame = 0


Query: 66  DLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTSFTSPRKVNMCSWLYRI 125
           +L YQSGF NHFSSEAI GALP  QNSPL+CPYGLYAEQISGTSFTSPRK+N  SWLYR+
Sbjct: 10  ELKYQSGFGNHFSSEAIAGALPLDQNSPLLCPYGLYAEQISGTSFTSPRKLNQRSWLYRV 69

Query: 126 KPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPDSPLDFVDGLYTVCGAG 185
           KPSVTHEPF+PR+P ++KL+SEF+ASN  + PTQLRWRP ++PDS +DFVDGL+T+CGAG
Sbjct: 70  KPSVTHEPFKPRVPAHKKLVSEFDASNSRTNPTQLRWRPEDIPDSEIDFVDGLFTICGAG 129

Query: 186 SSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLVVTFIENYERSGLQSEY 245
           SSFLRHGFAIHMY AN  M++ AFCNADGDFL+VPQ+G                      
Sbjct: 130 SSFLRHGFAIHMYVANTGMKDSAFCNADGDFLLVPQTGR--------------------- 189

Query: 246 FYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIITECGRLEVSPGEIVVLP 305
                                                  LWI TECGRL V+PGEI V+P
Sbjct: 190 ---------------------------------------LWIETECGRLLVTPGEIAVIP 249

Query: 306 QGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFENISRPGY 365
           QGFRF + LPDG SRGYVAEI+G+HFQLPDLGPIGANGLAA RDFLAP AWFE+  RP Y
Sbjct: 250 QGFRFSIDLPDGKSRGYVAEIYGAHFQLPDLGPIGANGLAASRDFLAPTAWFEDGLRPEY 309

Query: 366 TIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSINTVLTA 425
           TIVQK+GGELFTA QDFSPFNVVAWHGNYVPYKYDL KFCPYNTVL DH DPSINTVLTA
Sbjct: 310 TIVQKFGGELFTAKQDFSPFNVVAWHGNYVPYKYDLKKFCPYNTVLLDHGDPSINTVLTA 369

Query: 426 PTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFLPGGAS 485
           PTDKPGVALLDFVIFPPRWLV+EHTFRPPYYHRNCMSEFMGLIYG YEAKADGFLPGGAS
Sbjct: 370 PTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGAYEAKADGFLPGGAS 429

Query: 486 LHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIPRVCSWALESPCMDHDY 545
           LH+CMTPHGPDT TYEATIAR N   P ++T TMAFMFES+LIPRVC WALESP +DHDY
Sbjct: 430 LHSCMTPHGPDTTTYEATIARVNAMAPSKLTGTMAFMFESALIPRVCHWALESPFLDHDY 441

Query: 546 YQCWIGLKSHFT 558
           YQCWIGLKSHF+
Sbjct: 490 YQCWIGLKSHFS 441

[Close]

BLAST of Carg13174 vs. Swiss-Prot
Match: sp|Q9ZRA2|HGD_ARATH (Homogentisate 1,2-dioxygenase OS=Arabidopsis thaliana OX=3702 GN=HGO PE=2 SV=2)

HSP 1 Score: 758.1 bits (1956), Expect = 7.4e-218
Identity = 355/492 (72.15%), Postives = 389/492 (79.07%), Query Frame = 0


Query: 66  DLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTSFTSPRKVNMCSWLYRI 125
           +L YQSGF NHFSSEAI GALP  QNSPL+CPYGLYAEQISGTSFTSPRK+N  SWLYR+
Sbjct: 10  ELKYQSGFGNHFSSEAIAGALPLDQNSPLLCPYGLYAEQISGTSFTSPRKLNQRSWLYRV 69

Query: 126 KPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPDSPLDFVDGLYTVCGAG 185
           KPSVTHEPF+PR+P ++KL+SEF+ASN  + PTQLRWRP ++PDS +DFVDGL+T+CGAG
Sbjct: 70  KPSVTHEPFKPRVPAHKKLVSEFDASNSRTNPTQLRWRPEDIPDSEIDFVDGLFTICGAG 129

Query: 186 SSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLVVTFIENYERSGLQSEY 245
           SSFLRHGFAIHMY AN  M++ AFCNADGDFL+VPQ+G                      
Sbjct: 130 SSFLRHGFAIHMYVANTGMKDSAFCNADGDFLLVPQTGR--------------------- 189

Query: 246 FYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIITECGRLEVSPGEIVVLP 305
                                                  LWI TECGRL V+PGEI V+P
Sbjct: 190 ---------------------------------------LWIETECGRLLVTPGEIAVIP 249

Query: 306 QGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFENISRPGY 365
           QGFRF + LPDG SRGYVAEI+G+HFQLPDLGPIGANGLAA RDFLAP AWFE+  RP Y
Sbjct: 250 QGFRFSIDLPDGKSRGYVAEIYGAHFQLPDLGPIGANGLAASRDFLAPTAWFEDGLRPEY 309

Query: 366 TIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSINTVLTA 425
           TIVQK+GGELFTA QDFSPFNVVAWHGNYVPYKYDL KFCPYNTVL DH DPSINTVLTA
Sbjct: 310 TIVQKFGGELFTAKQDFSPFNVVAWHGNYVPYKYDLKKFCPYNTVLLDHGDPSINTVLTA 369

Query: 426 PTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFLPGGAS 485
           PTDKPGVALLDFVIFPPRWLV+EHTFRPPYYHRNCMSEFMGLIYG YEAKADGFLPGGAS
Sbjct: 370 PTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGAYEAKADGFLPGGAS 429

Query: 486 LHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIPRVCSWALESPCMDHDY 545
           LH+CMTPHGPDT TYEATIAR N   P ++T TMAFMFES+LIPRVC WALESP +DHDY
Sbjct: 430 LHSCMTPHGPDTTTYEATIARVNAMAPSKLTGTMAFMFESALIPRVCHWALESPFLDHDY 441

Query: 546 YQCWIGLKSHFT 558
           YQCWIGLKSHF+
Sbjct: 490 YQCWIGLKSHFS 441

[Close]

BLAST of Carg13174 vs. Swiss-Prot
Match: sp|Q5VRH4|HGD_ORYSJ (Homogentisate 1,2-dioxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=HGO PE=2 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 1.0e-206
Identity = 346/513 (67.45%), Postives = 389/513 (75.83%), Query Frame = 0


Query: 51  AAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTSF 110
           AAQ+  +  GG    +  Y SG  N  SSEA+ G LP+ QNSPL+CP GLYAEQ+SGT F
Sbjct: 9   AAQNEQQEKGG---LEYVYLSGLGNSLSSEAVAGTLPRGQNSPLVCPLGLYAEQLSGTPF 68

Query: 111 TSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFN--ASNCSSTPTQLRWRPAEVP 170
           T+PR  N+ +WLYRIKPSVTHEPF PR P + +LI +F+   ++  +TPTQLRWRPA+VP
Sbjct: 69  TAPRARNLRTWLYRIKPSVTHEPFHPRRPAHPRLIGDFDRTTTDTVATPTQLRWRPADVP 128

Query: 171 --DSPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSEL 230
               PLDF+DGLYTVCGAGSSFLRHG+AIHMY ANKSM+ CAFCNADGDFLIVPQ G   
Sbjct: 129 PHHPPLDFIDGLYTVCGAGSSFLRHGYAIHMYAANKSMDGCAFCNADGDFLIVPQQGK-- 188

Query: 231 LLVVTFIENYERSGLQSEYFYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLW 290
                                                                     L 
Sbjct: 189 ----------------------------------------------------------LL 248

Query: 291 IITECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAA 350
           I TECG+L V PGEIVV+PQGFRF V LPDGPSRGYV+EIFG+HFQLPDLGPIGANGLA+
Sbjct: 249 ITTECGKLLVPPGEIVVIPQGFRFAVDLPDGPSRGYVSEIFGTHFQLPDLGPIGANGLAS 308

Query: 351 PRDFLAPVAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCP 410
            RDFL+P AWFE + RPGYTIVQKYGGELFTA QDFSPFNVVAWHGNYVPYKYDLSKFCP
Sbjct: 309 ARDFLSPTAWFEQVHRPGYTIVQKYGGELFTATQDFSPFNVVAWHGNYVPYKYDLSKFCP 368

Query: 411 YNTVLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMG 470
           +NTVLFDH+DPS+NTVLTAPTDKPGVALLDFVIFPPRWLV+E+TFRPPYYHRNCMSEFMG
Sbjct: 369 FNTVLFDHADPSVNTVLTAPTDKPGVALLDFVIFPPRWLVAENTFRPPYYHRNCMSEFMG 428

Query: 471 LIYGGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESS 530
           LIYG YEAKADGFLPGGASLH+CMTPHGPDTKTYEATI+R +   P R++ T+AFMFES+
Sbjct: 429 LIYGIYEAKADGFLPGGASLHSCMTPHGPDTKTYEATISRPDANEPSRLSGTLAFMFESA 458

Query: 531 LIPRVCSWALESPCMDHDYYQCWIGLKSHFTSE 560
           LIPRVC WAL+SP  D DYYQCWIGLKSHF+ +
Sbjct: 489 LIPRVCQWALDSPSRDLDYYQCWIGLKSHFSHD 458

[Close]

BLAST of Carg13174 vs. Swiss-Prot
Match: sp|Q54QI4|HGD_DICDI (Homogentisate 1,2-dioxygenase OS=Dictyostelium discoideum OX=44689 GN=hgd PE=2 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 4.0e-139
Identity = 258/492 (52.44%), Postives = 316/492 (64.23%), Query Frame = 0


Query: 66  DLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTSFTSPRKVNMCSWLYRI 125
           D  YQSGF N F SEAI G LP+ +N+P  CP  LYAEQ+SG +FT+PR     SWLYRI
Sbjct: 7   DYEYQSGFGNSFESEAIKGTLPKGRNAPQNCPLDLYAEQLSGNAFTAPRHTQQRSWLYRI 66

Query: 126 KPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEV-PDSPLDFVDGLYTVCGA 185
           +PSV H P +   P +  L+ + N  N    P QLRW+P  +  D P DFV+GL T+ GA
Sbjct: 67  RPSVCHTPLK---PIDSGLVCDLN--NLHVDPNQLRWKPFPITEDKPHDFVEGLITIAGA 126

Query: 186 GSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLVVTFIENYERSGLQSE 245
           G + +RHG AIH+YTA KSMEN +F N+DGDFLIVPQ G+                    
Sbjct: 127 GHASVRHGLAIHIYTATKSMENKSFYNSDGDFLIVPQQGT-------------------- 186

Query: 246 YFYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIITECGRLEVSPGEIVVL 305
                                                   L I TE G ++V  GEI V+
Sbjct: 187 ----------------------------------------LDIQTEFGFMKVKSGEICVI 246

Query: 306 PQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFENISRPG 365
            +G  F V + +GP+RGY+ E+FGSHF+LPDLGPIGANGLA PRDFL+PVA +E      
Sbjct: 247 QRGITFSVNV-EGPTRGYICEVFGSHFKLPDLGPIGANGLANPRDFLSPVAAYEKKEGIE 306

Query: 366 YTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSINTVLT 425
           +T + K+ G+LF+A Q +SPFNVVAWHGNY PYKYDLS FC  N+V FDH DPSI TVLT
Sbjct: 307 HTKINKFLGKLFSATQTYSPFNVVAWHGNYCPYKYDLSLFCVVNSVSFDHLDPSIFTVLT 366

Query: 426 APTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFLPGGA 485
           APT++ GVA  DFVIFPPRWLV E+TFRPPY+HRNCMSEFMGLI G YEAK +GFLPGG 
Sbjct: 367 APTNEVGVAAADFVIFPPRWLVQENTFRPPYFHRNCMSEFMGLIRGVYEAKKEGFLPGGG 426

Query: 486 SLHNCMTPHGPDTKTYEATIARGNEAGPYRITD-TMAFMFESSLIPRVCSWALESPCMDH 545
           SLH+CMTPHGPD+ T+ A I    E  P +I D  +AFMFESSLI  +  +A ++  +D 
Sbjct: 427 SLHSCMTPHGPDSDTFYAAIKA--ELKPTKIPDVALAFMFESSLILGISDYAKKN-FIDD 429

Query: 546 DYYQCWIGLKSH 556
           DY++CW GLK +
Sbjct: 487 DYWKCWQGLKDN 429

[Close]

BLAST of Carg13174 vs. Swiss-Prot
Match: sp|Q93099|HGD_HUMAN (Homogentisate 1,2-dioxygenase OS=Homo sapiens OX=9606 GN=HGD PE=1 SV=2)

HSP 1 Score: 489.2 bits (1258), Expect = 6.4e-137
Identity = 255/501 (50.90%), Postives = 313/501 (62.48%), Query Frame = 0


Query: 65  SDLAYQSGFNNHFSSE--AIPGALPQLQNSPLICPYGLYAEQISGTSFTSPRKVNMCSWL 124
           ++L Y SGF N  SSE    PG+LP+ QN+P +CPY LYAEQ+SG++FT PR  N  SWL
Sbjct: 2   AELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSWL 61

Query: 125 YRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVP---DSPLDFVDGLY 184
           YRI PSV+H+PF      +E  ++  N       P QLRW+P E+P      +DFV GL+
Sbjct: 62  YRILPSVSHKPFE---SIDEGQVTH-NWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLH 121

Query: 185 TVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLVVTFIENYERS 244
           T+CGAG     +G AIH++  N SMEN  F N+DGDFLIVPQ G+               
Sbjct: 122 TLCGAGDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGN--------------- 181

Query: 245 GLQSEYFYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIITECGRLEVSPG 304
                                                        L I TE G++ V P 
Sbjct: 182 ---------------------------------------------LLIYTEFGKMLVQPN 241

Query: 305 EIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFEN 364
           EI V+ +G RF + + +  +RGY+ E++G HF+LPDLGPIGANGLA PRDFL P+AW+E+
Sbjct: 242 EICVIQRGMRFSIDVFE-ETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPIAWYED 301

Query: 365 ISRP-GYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPS 424
              P GYT++ KY G+LF A QD SPFNVVAWHGNY PYKY+L  F   N+V FDH+DPS
Sbjct: 302 RQVPGGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPS 361

Query: 425 INTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIYGGYEAKADG 484
           I TVLTA + +PGVA+ DFVIFPPRW V++ TFRPPYYHRNCMSEFMGLI G YEAK  G
Sbjct: 362 IFTVLTAKSVRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCMSEFMGLIRGHYEAKQGG 421

Query: 485 FLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITD-TMAFMFESSLIPRVCSWALE 544
           FLPGG SLH+ MTPHGPD   +E   A   +  P RI D TMAFMFESSL   V  W L+
Sbjct: 422 FLPGGGSLHSTMTPHGPDADCFEK--ASKVKLAPERIADGTMAFMFESSLSLAVTKWGLK 435

Query: 545 -SPCMDHDYYQCWIGLKSHFT 558
            S C+D +Y++CW  LKSHFT
Sbjct: 482 ASRCLDENYHKCWEPLKSHFT 435

[Close]

BLAST of Carg13174 vs. Swiss-Prot
Match: sp|O09173|HGD_MOUSE (Homogentisate 1,2-dioxygenase OS=Mus musculus OX=10090 GN=Hgd PE=1 SV=2)

HSP 1 Score: 486.1 bits (1250), Expect = 5.4e-136
Identity = 251/501 (50.10%), Postives = 309/501 (61.68%), Query Frame = 0


Query: 65  SDLAYQSGFNNHFSSE--AIPGALPQLQNSPLICPYGLYAEQISGTSFTSPRKVNMCSWL 124
           ++L Y SGF N  +SE    PG+LP+ QN+P +CPY LYAEQ+SG++FT PR  N  SWL
Sbjct: 2   AELKYISGFGNECASEDPRCPGSLPKGQNNPQVCPYNLYAEQLSGSAFTCPRNTNKRSWL 61

Query: 125 YRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVP---DSPLDFVDGLY 184
           YRI PSV+H+PF       ++     N       P QLRW+P E+P   +  +DFV GLY
Sbjct: 62  YRILPSVSHKPFE----SIDQGHVTHNWDEVGPDPNQLRWKPFEIPKASEKKVDFVSGLY 121

Query: 185 TVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLVVTFIENYERS 244
           T+CGAG     +G A+H++  N SMEN  F N+DGDFLIVPQ G                
Sbjct: 122 TLCGAGDIKSNNGLAVHIFLCNSSMENRCFYNSDGDFLIVPQKGK--------------- 181

Query: 245 GLQSEYFYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIITECGRLEVSPG 304
                                                        L I TE G++ + P 
Sbjct: 182 ---------------------------------------------LLIYTEFGKMSLQPN 241

Query: 305 EIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFEN 364
           EI V+ +G RF V + +  +RGY+ E++G HF+LPDLGPIGANGLA PRDFL PVAW+E+
Sbjct: 242 EICVIQRGMRFSVDVFE-ETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPVAWYED 301

Query: 365 ISRP-GYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPS 424
              P GYT++ K+ G+LF   QD SPFNVVAWHGNY PYKY+L  F   N V FDH+DPS
Sbjct: 302 RRVPGGYTVINKFQGKLFACKQDVSPFNVVAWHGNYTPYKYNLENFMVINAVAFDHADPS 361

Query: 425 INTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIYGGYEAKADG 484
           I TVLTA + +PGVA+ DFVIFPPRW V++ TFRPPYYHRNCMSEFMGLI G YEAK  G
Sbjct: 362 IFTVLTAKSLRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCMSEFMGLIKGHYEAKQGG 421

Query: 485 FLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITD-TMAFMFESSLIPRVCSWALE 544
           FLPGG SLH+ MTPHGPD   +E   A   +  P RI D TMAFMFESSL   V  W L+
Sbjct: 422 FLPGGGSLHSAMTPHGPDADCFEK--ASKAKLEPERIADGTMAFMFESSLSLAVTKWGLK 435

Query: 545 S-PCMDHDYYQCWIGLKSHFT 558
           +  C+D +YY+CW  L+SHFT
Sbjct: 482 TCSCLDENYYKCWEPLRSHFT 435

[Close]

BLAST of Carg13174 vs. TrEMBL
Match: tr|A0A0A0KYZ6|A0A0A0KYZ6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G088750 PE=4 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 3.2e-258
Identity = 428/530 (80.75%), Postives = 448/530 (84.53%), Query Frame = 0


Query: 50  MAAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS 109
           MAAQSVGE DG +FPSDL Y SGFNNHFSSEAIPGALPQ QNSPLICP+GLYAEQISGTS
Sbjct: 1   MAAQSVGETDGTDFPSDLPYLSGFNNHFSSEAIPGALPQSQNSPLICPFGLYAEQISGTS 60

Query: 110 FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPD 169
           FTSPRK N+CSWLYRIKPSVTHEPFR RLPKNEKLISEFNASNCSSTPTQLRW+PA+ PD
Sbjct: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCSSTPTQLRWKPADFPD 120

Query: 170 SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLV 229
           SP+DFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSG      
Sbjct: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGK----- 180

Query: 230 VTFIENYERSGLQSEYFYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIIT 289
                                                                  LWIIT
Sbjct: 181 -------------------------------------------------------LWIIT 240

Query: 290 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 349
           ECGRLEVSPGE+VVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD
Sbjct: 241 ECGRLEVSPGEVVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 300

Query: 350 FLAPVAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 409
           FLAPVAWFEN  RPGYTI+QK+GGELFTAIQDFSPFNVVAWHGNYVPYKYDL KFCPYNT
Sbjct: 301 FLAPVAWFENSPRPGYTIIQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLCKFCPYNT 360

Query: 410 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY 469
           VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLV+EHTFRPPYYHRNCMSEFMGLIY
Sbjct: 361 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 420

Query: 470 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 529
           GGYEAKADGF+PGGASLH+CMTPHGPDTKTYEATIARGN+AGP++I+ TMAFMFESSLIP
Sbjct: 421 GGYEAKADGFVPGGASLHSCMTPHGPDTKTYEATIARGNDAGPHKISGTMAFMFESSLIP 469

Query: 530 RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPQKVKTDVENGRQ 580
           RVCSWALESP +DHDYYQCWIGLKSHF +E    D DPQKV+ + ENGRQ
Sbjct: 481 RVCSWALESPFIDHDYYQCWIGLKSHFKNE-AIGDTDPQKVRIESENGRQ 469

[Close]

BLAST of Carg13174 vs. TrEMBL
Match: tr|A0A1S3BS32|A0A1S3BS32_CUCME (homogentisate 1,2-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103492917 PE=4 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 7.8e-257
Identity = 434/556 (78.06%), Postives = 457/556 (82.19%), Query Frame = 0


Query: 24  PVPFSGFNSLHNRSSSSSAFYSSSFSMAAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIP 83
           PV  SG ++L NRSS S        SMAAQSVGE +G +FPSDL Y SGFNNHFSSEAIP
Sbjct: 4   PVISSGSDNLCNRSSYS--------SMAAQSVGETEGRDFPSDLPYLSGFNNHFSSEAIP 63

Query: 84  GALPQLQNSPLICPYGLYAEQISGTSFTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEK 143
           GALPQ QNSPL CP+GLYAEQISGTSFTSPRK N+CSWLYRIKPSVTHEPFR RLPKNEK
Sbjct: 64  GALPQSQNSPLNCPFGLYAEQISGTSFTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEK 123

Query: 144 LISEFNASNCSSTPTQLRWRPAEVPDSPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKS 203
           LISEFNASNCSSTPTQLRW+PA+ PDSP+DFVDGL+TVCGAGSSFLRHGFAIHMYTANKS
Sbjct: 124 LISEFNASNCSSTPTQLRWKPADFPDSPVDFVDGLHTVCGAGSSFLRHGFAIHMYTANKS 183

Query: 204 MENCAFCNADGDFLIVPQSGSELLLVVTFIENYERSGLQSEYFYSENLSLLYIESEGNYI 263
           MENCAFCNADGDFLIVPQ+G                                        
Sbjct: 184 MENCAFCNADGDFLIVPQTGR--------------------------------------- 243

Query: 264 PVLKVLRKQDQTLIEYSYSIGLWIITECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYV 323
                                LWI TECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYV
Sbjct: 244 ---------------------LWITTECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYV 303

Query: 324 AEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFENISRPGYTIVQKYGGELFTAIQDFS 383
           AEIFG HFQLPDLGPIGANGLAAPRDFLAPVAWFEN  RPGYT++QK+GGELFTAIQDFS
Sbjct: 304 AEIFGCHFQLPDLGPIGANGLAAPRDFLAPVAWFENSPRPGYTVIQKFGGELFTAIQDFS 363

Query: 384 PFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPR 443
           PFNVVAWHGNYVPYKYDL KFCPYNTVLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPR
Sbjct: 364 PFNVVAWHGNYVPYKYDLCKFCPYNTVLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPR 423

Query: 444 WLVSEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFLPGGASLHNCMTPHGPDTKTYEAT 503
           WLV+EHTFRPPYYHRNCMSEFMGLIYGGYEAKADGF+PGGASLH+CMTPHGPDTKTYEAT
Sbjct: 424 WLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFVPGGASLHSCMTPHGPDTKTYEAT 483

Query: 504 IARGNEAGPYRITDTMAFMFESSLIPRVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTR 563
           IARGN+AGP++I+ TMAFMFESSLIPRVCSWALESP MDHDYYQCWIGLKSHF +E    
Sbjct: 484 IARGNDAGPHKISGTMAFMFESSLIPRVCSWALESPFMDHDYYQCWIGLKSHFKNE-AIG 490

Query: 564 DMDPQKVKTDVENGRQ 580
           D DPQKV+   ENGRQ
Sbjct: 544 DTDPQKVRIKSENGRQ 490

[Close]

BLAST of Carg13174 vs. TrEMBL
Match: tr|A0A1S3Y096|A0A1S3Y096_TOBAC (LOW QUALITY PROTEIN: homogentisate 1,2-dioxygenase-like OS=Nicotiana tabacum OX=4097 GN=LOC107770720 PE=4 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 7.9e-233
Identity = 384/502 (76.49%), Postives = 409/502 (81.47%), Query Frame = 0


Query: 63  FPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTSFTSPRKVNMCSWL 122
           FPSDL YQSGF NHFSSEAI GALPQ QNSPLICP+GLYAEQISGTSFTSPRK+N  SWL
Sbjct: 16  FPSDLEYQSGFGNHFSSEAIVGALPQGQNSPLICPFGLYAEQISGTSFTSPRKLNQRSWL 75

Query: 123 YRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPDSPLDFVDGLYTVC 182
           YRIK SVTHEPFRPRLP++EKL+SEF+ SN S+TPTQLRW+P E+P++P DF+DGLYT+C
Sbjct: 76  YRIKLSVTHEPFRPRLPRHEKLVSEFDQSNSSATPTQLRWKPVEIPETPTDFIDGLYTIC 135

Query: 183 GAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLVVTFIENYERSGLQ 242
           GAGSS+LRHGFAIHMYTANKSMENCAFCNADGDFLIVPQ G                   
Sbjct: 136 GAGSSYLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQQGR------------------ 195

Query: 243 SEYFYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIITECGRLEVSPGEIV 302
                                                     LWI TECGRL+VSPGEIV
Sbjct: 196 ------------------------------------------LWITTECGRLQVSPGEIV 255

Query: 303 VLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFENISR 362
           +LPQGFRF V LPDGPSRGYVAEIFG+H QLPDLGPIGANGLAAPRDFL PVAW+E+ S+
Sbjct: 256 ILPQGFRFSVDLPDGPSRGYVAEIFGTHLQLPDLGPIGANGLAAPRDFLVPVAWYEDGSQ 315

Query: 363 PGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSINTV 422
           PGYTIVQKYGGELFTA QDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVL DHSDPSINTV
Sbjct: 316 PGYTIVQKYGGELFTAKQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLIDHSDPSINTV 375

Query: 423 LTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFLPG 482
           LTAPTDKPGVALLDFVIFPPRWLV+EHTFRPPYYHRNCMSEFMGLIYGGYEAKADGF PG
Sbjct: 376 LTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFHPG 435

Query: 483 GASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIPRVCSWALESPCMD 542
           GASLH+CMTPHGPDTKTYEATIA GNEAGP++I DTMAFMFES LIPRVC WALESP MD
Sbjct: 436 GASLHSCMTPHGPDTKTYEATIALGNEAGPHKIADTMAFMFESCLIPRVCPWALESPFMD 457

Query: 543 HDYYQCWIGLKSHFTSEGTTRD 565
           HDYYQCWIGLKSHF+      D
Sbjct: 496 HDYYQCWIGLKSHFSGLSMKED 457

[Close]

BLAST of Carg13174 vs. TrEMBL
Match: tr|Q9M6U1|Q9M6U1_SOLLC (Homogentisate 1,2-dioxygenase (Fragment) OS=Solanum lycopersicum OX=4081 GN=HGO PE=2 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.0e-232
Identity = 385/512 (75.20%), Postives = 413/512 (80.66%), Query Frame = 0


Query: 62  NFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTSFTSPRKVNMCSW 121
           NFPSDL YQ+GF NHFSSEAI GALPQ QNSPLICP+GLYAEQISGTSFTSPRK+N  SW
Sbjct: 6   NFPSDLEYQTGFGNHFSSEAIVGALPQGQNSPLICPFGLYAEQISGTSFTSPRKLNQRSW 65

Query: 122 LYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPDSPLDFVDGLYTV 181
           LYRIKPSVTHEPFRPR+P++EKL+SEFN SN S+TPTQLRW+P E+P++P DF+DGLYT+
Sbjct: 66  LYRIKPSVTHEPFRPRMPRHEKLVSEFNQSNSSATPTQLRWKPVEIPETPTDFIDGLYTI 125

Query: 182 CGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLVVTFIENYERSGL 241
           CGAGSS+LRHGFAIHMYTANKSMEN AFCNADGDFLIVPQ G                  
Sbjct: 126 CGAGSSYLRHGFAIHMYTANKSMENSAFCNADGDFLIVPQKGR----------------- 185

Query: 242 QSEYFYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIITECGRLEVSPGEI 301
                                                      LWI TECGRL+V PGEI
Sbjct: 186 -------------------------------------------LWITTECGRLQVCPGEI 245

Query: 302 VVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFENIS 361
           V+LPQG+RF V LPDGPSRGYVAE FG+H QLPDLGPIGANGLAAPRDFL PVAW+ + S
Sbjct: 246 VILPQGYRFAVDLPDGPSRGYVAETFGTHLQLPDLGPIGANGLAAPRDFLVPVAWYGDGS 305

Query: 362 RPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSINT 421
           RPGYTIVQKYGGELFTA QDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVL DHSDPSINT
Sbjct: 306 RPGYTIVQKYGGELFTAKQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLMDHSDPSINT 365

Query: 422 VLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFLP 481
           VLTAPTDKPGVALLDFVIFPPRWLV+EHTFRPPYYHRNCMSEFMGLIYGGYEAKADGF P
Sbjct: 366 VLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFHP 425

Query: 482 GGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIPRVCSWALESPCM 541
           GGASLH+CMTPHGPDTKT+EATIA GNEAGP+RI DTMAFMFES L+PRVC WALESP M
Sbjct: 426 GGASLHSCMTPHGPDTKTFEATIALGNEAGPHRIADTMAFMFESCLVPRVCPWALESPFM 457

Query: 542 DHDYYQCWIGLKSHFTSEGTTRD-MDPQKVKT 573
           DHDYYQCWIGLKSHF+      D +D QK KT
Sbjct: 486 DHDYYQCWIGLKSHFSGLSMNEDNVDLQKGKT 457

[Close]

BLAST of Carg13174 vs. TrEMBL
Match: tr|A0A0V0IDM4|A0A0V0IDM4_SOLCH (Putative homogentisate 1,2-dioxygenase-like OS=Solanum chacoense OX=4108 PE=4 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 1.3e-232
Identity = 386/511 (75.54%), Postives = 411/511 (80.43%), Query Frame = 0


Query: 62  NFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTSFTSPRKVNMCSW 121
           NFPSDL YQ+GF NHFSSEAI GALPQ QNSPLICP+GLYAEQISGTSFTSPRK+N  SW
Sbjct: 9   NFPSDLEYQTGFGNHFSSEAIVGALPQGQNSPLICPFGLYAEQISGTSFTSPRKLNQRSW 68

Query: 122 LYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPDSPLDFVDGLYTV 181
           LYRIKPSVTHEPFRPR+P++EKL+SEFN SN S+TPTQLRW+P E+P++P DF+DGLYT+
Sbjct: 69  LYRIKPSVTHEPFRPRMPRHEKLVSEFNQSNSSATPTQLRWKPVEIPETPTDFIDGLYTI 128

Query: 182 CGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGSELLLVVTFIENYERSGL 241
           CGAGSS+LRHGFAIHMYTANKSMEN AFCNADGDFLIVPQ G                  
Sbjct: 129 CGAGSSYLRHGFAIHMYTANKSMENSAFCNADGDFLIVPQKGR----------------- 188

Query: 242 QSEYFYSENLSLLYIESEGNYIPVLKVLRKQDQTLIEYSYSIGLWIITECGRLEVSPGEI 301
                                                      LWI TECGRL+V PGEI
Sbjct: 189 -------------------------------------------LWITTECGRLQVCPGEI 248

Query: 302 VVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRDFLAPVAWFENIS 361
           V LPQG+RF V LPDGPSRGYVAE FG+H QLPDLGPIGANGLAAPRDFL PVAW+E+ S
Sbjct: 249 VTLPQGYRFAVDLPDGPSRGYVAETFGTHLQLPDLGPIGANGLAAPRDFLVPVAWYEDGS 308

Query: 362 RPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLFDHSDPSINT 421
           RPGY IVQKYGGELFTA QDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVL DHSDPSINT
Sbjct: 309 RPGYAIVQKYGGELFTAKQDFSPFNVVAWHGNYVPYKYDLSKFCPYNTVLMDHSDPSINT 368

Query: 422 VLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFLP 481
           VLTAPTDKPGVALLDFVIFPPRWLV+EHTFRPPYYHRNCMSEFMGLIYGGYEAKADGF P
Sbjct: 369 VLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFHP 428

Query: 482 GGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIPRVCSWALESPCM 541
           GGASLH+CMTPHGPDTKTYEATIA GNEAGP+RI DTMAFMFES LIPRVC WALESP M
Sbjct: 429 GGASLHSCMTPHGPDTKTYEATIALGNEAGPHRIADTMAFMFESCLIPRVCPWALESPFM 459

Query: 542 DHDYYQCWIGLKSHFTSEGTTRD-MDPQKVK 572
           DHDYYQCWIGLKSHF+      D +D QK K
Sbjct: 489 DHDYYQCWIGLKSHFSGLSMNEDNVDLQKGK 459

The following BLAST results are available for this feature:

BLAST of Carg13174 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022980282.1	5.8e-304	87.07	homogentisate 1,2-dioxygenase [Cucurbita maxima]	[more]
XP_022942212.1	9.6e-283	88.16	homogentisate 1,2-dioxygenase [Cucurbita moschata]	[more]
XP_023550715.1	1.4e-281	88.16	homogentisate 1,2-dioxygenase [Cucurbita pepo subsp. pepo]	[more]
XP_004137214.1	4.8e-258	80.75	PREDICTED: homogentisate 1,2-dioxygenase [Cucumis sativus] >KGN53602.1 hypotheti...	[more]
XP_022142871.1	5.3e-257	80.57	homogentisate 1,2-dioxygenase [Momordica charantia]	[more]

BLAST of Carg13174 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G54080.1	4.1e-219	72.15	homogentisate 1,2-dioxygenase	[more]

BLAST of Carg13174 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9ZRA2\|HGD_ARATH	7.4e-218	72.15	Homogentisate 1,2-dioxygenase OS=Arabidopsis thaliana OX=3702 GN=HGO PE=2 SV=2	[more]
sp\|Q5VRH4\|HGD_ORYSJ	1.0e-206	67.45	Homogentisate 1,2-dioxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=HGO PE...	[more]
sp\|Q54QI4\|HGD_DICDI	4.0e-139	52.44	Homogentisate 1,2-dioxygenase OS=Dictyostelium discoideum OX=44689 GN=hgd PE=2 S...	[more]
sp\|Q93099\|HGD_HUMAN	6.4e-137	50.90	Homogentisate 1,2-dioxygenase OS=Homo sapiens OX=9606 GN=HGD PE=1 SV=2	[more]
sp\|O09173\|HGD_MOUSE	5.4e-136	50.10	Homogentisate 1,2-dioxygenase OS=Mus musculus OX=10090 GN=Hgd PE=1 SV=2	[more]

BLAST of Carg13174 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KYZ6\|A0A0A0KYZ6_CUCSA	3.2e-258	80.75	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G088750 PE=4 SV=1	[more]
tr\|A0A1S3BS32\|A0A1S3BS32_CUCME	7.8e-257	78.06	homogentisate 1,2-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103492917 PE=4 SV=1	[more]
tr\|A0A1S3Y096\|A0A1S3Y096_TOBAC	7.9e-233	76.49	LOW QUALITY PROTEIN: homogentisate 1,2-dioxygenase-like OS=Nicotiana tabacum OX=...	[more]
tr\|Q9M6U1\|Q9M6U1_SOLLC	1.0e-232	75.20	Homogentisate 1,2-dioxygenase (Fragment) OS=Solanum lycopersicum OX=4081 GN=HGO ...	[more]
tr\|A0A0V0IDM4\|A0A0V0IDM4_SOLCH	1.3e-232	75.54	Putative homogentisate 1,2-dioxygenase-like OS=Solanum chacoense OX=4108 PE=4 SV...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process
GO:0006570	tyrosine metabolic process
GO:0006559	L-phenylalanine catabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0004411	homogentisate 1,2-dioxygenase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR011051	RmlC_Cupin_sf
IPR014710	RmlC-like_jellyroll
IPR005708	Homogentis_dOase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006559	L-phenylalanine catabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0042207	styrene catabolic process
biological_process	GO:0006570	tyrosine metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004411	homogentisate 1,2-dioxygenase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13174-RA	Carg13174-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005708	Homogentisate 1,2-dioxygenase	PFAM	PF04209	HgmA	coord: 68..224 e-value: 3.5E-62 score: 210.4 coord: 279..556 e-value: 7.4E-142 score: 472.9
IPR005708	Homogentisate 1,2-dioxygenase	PANTHER	PTHR11056	HOMOGENTISATE 1,2-DIOXYGENASE	coord: 65..227 coord: 284..558
IPR014710	RmlC-like jelly roll fold	GENE3D	G3DSA:2.60.120.10		coord: 389..527 e-value: 1.7E-60 score: 204.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 559..581
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 559..573
None	No IPR available	PANTHER	PTHR11056:SF0	HOMOGENTISATE 1,2-DIOXYGENASE	coord: 65..227 coord: 284..558
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	SSF51182	RmlC-like cupins	coord: 285..558
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	SSF51182	RmlC-like cupins	coord: 66..230

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg13174	Cla002425	Watermelon (97103) v1	carwmB0046
Carg13174	Csa4G088750	Cucumber (Chinese Long) v2	carcuB0045
Carg13174	MELO3C016089	Melon (DHL92) v3.5.1	carmeB0046
Carg13174	ClCG07G009900	Watermelon (Charleston Gray)	carwcgB0048
Carg13174	CSPI04G08190	Wild cucumber (PI 183967)	carcpiB0049
Carg13174	Cucsa.273420	Cucumber (Gy14) v1	carcgyB0055
Carg13174	CmaCh04G005620	Cucurbita maxima (Rimu)	carcmaB0055
Carg13174	CmoCh04G005990	Cucurbita moschata (Rifu)	carcmoB0058
Carg13174	Lsi07G003060	Bottle gourd (USVL1VR-Ls)	carlsiB041
Carg13174	Cp4.1LG01g01600	Cucurbita pepo (Zucchini)	carcpeB0061
Carg13174	CsGy4G008080	Cucumber (Gy14) v2	carcgybB0045
Carg13174	MELO3C016089.2	Melon (DHL92) v3.6.1	carmedB0048
Carg13174	CsaV3_4G008430	Cucumber (Chinese Long) v3	carcucB0050
Carg13174	Cla97C07G136610	Watermelon (97103) v2	carwmbB0046
Carg13174	Bhi01G002539	Wax gourd	carwgoB0054

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg13174	Silver-seed gourd	carcarB051
Carg13174	Cucurbita maxima (Rimu)	carcmaB0053
Carg13174	Cucurbita moschata (Rifu)	carcmoB0055
Carg13174	Cucurbita pepo (Zucchini)	carcpeB0059