Carg12862 (gene) Silver-seed gourd

NameCarg12862
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionPectinesterase
LocationCucurbita_argyrosperma_scaffold_043 : 783063 .. 784837 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATATATAATAAATCTCTAAATTTTTGTAAAAAAATATATGTTGTTTTGAATTGATCTACAAAACCAAGATTTGATGATGAACTAAAGTGTATTGTAATATGAACTTGCAGAGAGAAGGTATACATACCCATCACCAAGCCATATATTTCATTGATAGGAAACAGGAGACGAATGACAGACACTGTAATTACATCGAACAGCAAAGCTTCCGACAAAGATGTCAGCGGACTCGAGCTCGGGACATATAGAACAGCGACGGTTGCAATCGACTCCGACTACTTCTGTGCATCGGGAATCACATTCGAGGTTAGCAGCATAAACAACAAAGATAAACAAAGATAACTGAGTTTTGTAAGTTAAATGTGGTTTGGTTTGTAGAACACAGTTGTTTCAGCGCCTGGAGATAAGGGAAGACAAGCTGTGGCTCTAAGGATTTCAGGAGAGAAGGCTATGTTTTATAGAGTCAAGTTCTTGGGACAACAAGATACACTTTTGGATGATATGGGCACACACTATTTCTATCAATGTCACATTCAAGGGACTATTGATTTCATATTTGGTACGGCTAGATCGCTCTATGAGGTAACGAGTCTCGTTACTAGACATGTAGTTGATACCTTTCGAGTTTTGAACTTGTCGTCTGATGTTTGTCTGTTTTTTTTCTCTCTTGAAAACCAGCAATGTGTGATAACTTCCACGGCGGAGACCTTTGGAGCTATAGCAGCGCATCATCGGAGCTCGCCGGACGATGACACCGGGTTCTCTTTTGTGCGCTGCGTGATTAATGGAAGTGGCAGAGTACTCCTGGGAAGAGCATGGGGGACCTATTCAAGAACAATATATTCTTACTGTTATATTGAAGATATGATAAGTCCGTTAGGATGGAGCGACTGGAGCGACCCATCAAGACAAAGGTAAGGCAAATATACTTACAATAACGACCCCCCGACACATACACACACATACCATGACATTAACCGAGGTGGGCGTAAGTTGACCCGAACACTCATAAGAATCATAAATATCCCTCCACCTCTTGAATCAAGCTAATCTAATAGAAATTTTCTATTGTTATCAAGACAAAGTATACTTACAATGACGACCCCCCCCGACACACACACATCCCATGAGATTAACCGAGGTGGGTGTAGGTTGACCCGAACACTTACAAGAATTATAAATATCCCTCCACCTCTTGAATCAAGCTAATATTGTTATAAGACAAAGGTAAAAGGAAAAGTACACTTACAATAACGACCCCCCGACCCCCGACCCCCGACACATACACACACATCCTGTGAGATTAACCGAGGTGGGCATAGGTTGACCCGAACACTCACAAGAATTATAAATATCCCTCCACCTCATGAATCAAGCTAACCTAATAGAACTTTTCAATTGTTATCCACACACAGGACGGCAGTTTTTGGGCAATATAGATGCAGGGGCAGCGGAGCTAACACAAAAGGTTGGGTGCCATGGGCAAGGACTTTCACCTCCGAGGAAGTGAGACCTTACGTAGATAGGAACTTCATCAATGGAGAACAATGGCTTAACCTATGATGAGATATAACACAAAGAATATTAACTTTTGACACTTCCAATACAAAAAAACATATATAGAACAACCATCATGTTGAGTTCCAATGATGGAATAACAAACAAAAATGTAGCAACAATTACATCAACGCCAAAGAGTATTTAGAAACAAGCGTCTATGTTTAGAACATTTTATAATTCTTTCTAGTGGTTATCTACTTCCTTTTTTTGCTTA

mRNA sequence

TATATATAATAAATCTCTAAATTTTTGTAAAAAAATATATGTTGTTTTGAATTGATCTACAAAACCAAGATTTGATGATGAACTAAAGTGTATTGTAATATGAACTTGCAGAGAGAAGGTATACATACCCATCACCAAGCCATATATTTCATTGATAGGAAACAGGAGACGAATGACAGACACTGTAATTACATCGAACAGCAAAGCTTCCGACAAAGATGTCAGCGGACTCGAGCTCGGGACATATAGAACAGCGACGGTTGCAATCGACTCCGACTACTTCTGTGCATCGGGAATCACATTCGAGAACACAGTTGTTTCAGCGCCTGGAGATAAGGGAAGACAAGCTCAATGTGTGATAACTTCCACGGCGGAGACCTTTGGAGCTATAGCAGCGCATCATCGGAGCTCGCCGGACGATGACACCGGGTTCTCTTTTGTGCGCTGCGTGATTAATGGAAGTGGCAGAGTACTCCTGGGAAGAGCATGGGGGACCTATTCAAGAACAATATATTCTTACTGTTATATTGAAGATATGATAAGTCCGTTAGGATGGAGCGACTGGAGCGACCCATCAAGACAAAGGACGGCAGTTTTTGGGCAATATAGATGCAGGGGCAGCGGAGCTAACACAAAAGGTTGGGTGCCATGGGCAAGGACTTTCACCTCCGAGGAAGTGAGACCTTACGTAGATAGGAACTTCATCAATGGAGAACAATGGCTTAACCTATGATGAGATATAACACAAAGAATATTAACTTTTGACACTTCCAATACAAAAAAACATATATAGAACAACCATCATGTTGAGTTCCAATGATGGAATAACAAACAAAAATGTAGCAACAATTACATCAACGCCAAAGAGTATTTAGAAACAAGCGTCTATGTTTAGAACATTTTATAATTCTTTCTAGTGGTTATCTACTTCCTTTTTTTGCTTA

Coding sequence (CDS)

ATGACAGACACTGTAATTACATCGAACAGCAAAGCTTCCGACAAAGATGTCAGCGGACTCGAGCTCGGGACATATAGAACAGCGACGGTTGCAATCGACTCCGACTACTTCTGTGCATCGGGAATCACATTCGAGAACACAGTTGTTTCAGCGCCTGGAGATAAGGGAAGACAAGCTCAATGTGTGATAACTTCCACGGCGGAGACCTTTGGAGCTATAGCAGCGCATCATCGGAGCTCGCCGGACGATGACACCGGGTTCTCTTTTGTGCGCTGCGTGATTAATGGAAGTGGCAGAGTACTCCTGGGAAGAGCATGGGGGACCTATTCAAGAACAATATATTCTTACTGTTATATTGAAGATATGATAAGTCCGTTAGGATGGAGCGACTGGAGCGACCCATCAAGACAAAGGACGGCAGTTTTTGGGCAATATAGATGCAGGGGCAGCGGAGCTAACACAAAAGGTTGGGTGCCATGGGCAAGGACTTTCACCTCCGAGGAAGTGAGACCTTACGTAGATAGGAACTTCATCAATGGAGAACAATGGCTTAACCTATGA

Protein sequence

MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQAQCVITSTAETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL
BLAST of Carg12862 vs. NCBI nr
Match: XP_022936222.1 (pectinesterase QRT1 [Cucurbita moschata])

HSP 1 Score: 367.1 bits (941), Expect = 3.6e-98
Identity = 186/240 (77.50%), Postives = 186/240 (77.50%), Query Frame = 0

Query: 1   MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA- 60
           MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA 
Sbjct: 134 MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQAV 193

Query: 61  -----------------------------------------------------QCVITST 120
                                                                QCVITST
Sbjct: 194 ALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVITST 253

Query: 121 AETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPL 180
           AETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPL
Sbjct: 254 AETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPL 313

Query: 181 GWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 187
           GWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL
Sbjct: 314 GWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 373

BLAST of Carg12862 vs. NCBI nr
Match: XP_022975722.1 (pectinesterase QRT1 [Cucurbita maxima])

HSP 1 Score: 367.1 bits (941), Expect = 3.6e-98
Identity = 186/240 (77.50%), Postives = 186/240 (77.50%), Query Frame = 0

Query: 1   MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA- 60
           MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA 
Sbjct: 136 MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQAV 195

Query: 61  -----------------------------------------------------QCVITST 120
                                                                QCVITST
Sbjct: 196 ALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEQCVITST 255

Query: 121 AETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPL 180
           AETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPL
Sbjct: 256 AETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPL 315

Query: 181 GWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 187
           GWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL
Sbjct: 316 GWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 375

BLAST of Carg12862 vs. NCBI nr
Match: XP_023536329.1 (pectinesterase QRT1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 355.1 bits (910), Expect = 1.4e-94
Identity = 186/271 (68.63%), Postives = 186/271 (68.63%), Query Frame = 0

Query: 1   MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA- 60
           MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA 
Sbjct: 132 MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQAV 191

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 192 ALRISGEKAMFYRVKFLGQQDTLLDDMGTHYFYQCHIQGTIDFIFGTARSLYEVTVTRSR 251

Query: 121 ------------------------QCVITSTAETFGAIAAHHRSSPDDDTGFSFVRCVIN 180
                                   QCVITSTAETFGAIAAHHRSSPDDDTGFSFVRCVIN
Sbjct: 252 QVVDPFRVLNLSSDVCLFFSLENQQCVITSTAETFGAIAAHHRSSPDDDTGFSFVRCVIN 311

Query: 181 GSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTK 187
           GSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTK
Sbjct: 312 GSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTK 371

BLAST of Carg12862 vs. NCBI nr
Match: XP_008463039.1 (PREDICTED: pectinesterase QRT1 [Cucumis melo])

HSP 1 Score: 310.8 bits (795), Expect = 3.0e-81
Identity = 152/240 (63.33%), Postives = 172/240 (71.67%), Query Frame = 0

Query: 1   MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA- 60
           +T+TVIT N KASDK+++G+ELGTY+TATVAIDSDYFCA+GITFENT V+ PGDKGRQ  
Sbjct: 139 VTETVITWNDKASDKNINGVELGTYKTATVAIDSDYFCATGITFENTNVAKPGDKGRQGV 198

Query: 61  -----------------------------------------------------QCVITST 120
                                                                QCVITST
Sbjct: 199 ALRITGDKAVFYRVKFLGGQDTLLDDLGTHYFYQCHIRGSVDFIFGTARSLYEQCVITST 258

Query: 121 AETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPL 180
           AE++GAIAAHHR+SPDDDTGFSFVRCVINGSG+V LGRAWG YSRTIYS CYIED+I+PL
Sbjct: 259 AESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPL 318

Query: 181 GWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 187
           GWSDW+DPSRQRT VFGQY CRGSGANTK WVPWA+TF+ EEVRP+VDR +I GEQWLNL
Sbjct: 319 GWSDWNDPSRQRTVVFGQYNCRGSGANTKDWVPWAKTFSYEEVRPFVDRKYIKGEQWLNL 378

BLAST of Carg12862 vs. NCBI nr
Match: XP_004150299.1 (PREDICTED: pectinesterase QRT1 [Cucumis sativus])

HSP 1 Score: 308.5 bits (789), Expect = 1.5e-80
Identity = 152/240 (63.33%), Postives = 168/240 (70.00%), Query Frame = 0

Query: 1   MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA- 60
           +TDTVIT N KASDK + G+ELGTYRTATVAIDSDYFCA+GITFENTVV+ PGDKGRQ  
Sbjct: 120 VTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTVVAKPGDKGRQGV 179

Query: 61  -----------------------------------------------------QCVITST 120
                                                                QCVITST
Sbjct: 180 ALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQCVITST 239

Query: 121 AETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPL 180
           AE++GAIAAHHR+SPDDDTGFSFVRCVINGSG+V LGRAWG YSRTIYS CYIED+I+PL
Sbjct: 240 AESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPL 299

Query: 181 GWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 187
           GWSDW+DPSRQRT  FGQY CRGSGA+TK WV WA+TF  EEVRP+VDR +I GEQWLNL
Sbjct: 300 GWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRKYIKGEQWLNL 359

BLAST of Carg12862 vs. TAIR10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 228.0 bits (580), Expect = 4.7e-60
Identity = 113/238 (47.48%), Postives = 144/238 (60.50%), Query Frame = 0

Query: 3   DTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA--- 62
           DTVI+ + KASD    G ELGTYRTA+V+I+SD+FCA+ ITFENTVV+  G++GRQA   
Sbjct: 143 DTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQAVAL 202

Query: 63  ---------------------------------------------------QCVITSTAE 122
                                                               C I STA+
Sbjct: 203 RIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTAK 262

Query: 123 TFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGW 182
            +GAIAAHHR S  +DTGFSFV C I+G+G++ LGRAWG YSRT+YS C+I D+I+P+GW
Sbjct: 263 RYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVGW 322

Query: 183 SDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 187
           SDW  P RQR  +FG+Y CRG GA   G VPW++T T +EV+P++ R FI G+QWL L
Sbjct: 323 SDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLRL 380

BLAST of Carg12862 vs. TAIR10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 160.2 bits (404), Expect = 1.2e-39
Identity = 83/238 (34.87%), Postives = 119/238 (50.00%), Query Frame = 0

Query: 4   TVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTV-VSAPGDKGRQA--- 63
           T +     A   D  G  +GTY +A+ A++S +F A  ITF NT  V  PG  G+QA   
Sbjct: 146 TTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVAL 205

Query: 64  ---------------------------------------------------QCVITSTAE 123
                                                               C + + A+
Sbjct: 206 RVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIAD 265

Query: 124 TFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGW 183
             GA+ A  RSS  +DTGFSFV+C + G+G + LGRAWG +SR +++Y Y++++I P GW
Sbjct: 266 KLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGW 325

Query: 184 SDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 187
            +W DPSR+ T  +GQY+C G+GAN  G V WAR  T EE +P++   FI+G +W+ L
Sbjct: 326 YNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIKL 383

BLAST of Carg12862 vs. TAIR10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 138.3 bits (347), Expect = 4.9e-33
Identity = 77/237 (32.49%), Postives = 112/237 (47.26%), Query Frame = 0

Query: 4   TVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTV-VSAPGDKGRQA--- 63
           T I  + +ASD   +G +L TY+TA+V + ++YF A  I+F NT     PG +G QA   
Sbjct: 121 TAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAF 180

Query: 64  ---------------------------------------------------QCVITSTAE 123
                                                               C + S A 
Sbjct: 181 RISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIAS 240

Query: 124 TFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGW 183
            FG+IAAH R+ P++ TGF+FV C + G+G + +GRA G YSR +Y+Y Y + +++  GW
Sbjct: 241 RFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGW 300

Query: 184 SDWSDPS-RQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWL 185
            DW   S + +TA FG Y C G GA     V WAR    E   P++ ++F+NG  W+
Sbjct: 301 DDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWI 357

BLAST of Carg12862 vs. TAIR10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 125.6 bits (314), Expect = 3.3e-29
Identity = 50/120 (41.67%), Postives = 81/120 (67.50%), Query Frame = 0

Query: 65  STAETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMIS 124
           +T+   G+I A  R S D+ +GFSFV C I+GSG +LLGRAWG Y+  ++S  Y+  +I+
Sbjct: 266 NTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIIT 325

Query: 125 PLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWL 184
           P GW++W D ++++T  FG+++C G GA+ K  V + +  T  E   ++D +FI+G++WL
Sbjct: 326 PEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWL 385

BLAST of Carg12862 vs. TAIR10
Match: AT2G21610.1 (pectinesterase 11)

HSP 1 Score: 120.6 bits (301), Expect = 1.1e-27
Identity = 44/125 (35.20%), Postives = 79/125 (63.20%), Query Frame = 0

Query: 60  QCVITSTAETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYI 119
           +C + S +   G+I A  R+S  + +GF+F+ C + GSG   LGR WG YSR +++Y + 
Sbjct: 208 RCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFF 267

Query: 120 EDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFIN 179
            ++++P GW+ W D +++ T  +G+Y+C G GA+ +  V W++  + EE   ++ ++FI 
Sbjct: 268 SNVVAPQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIG 327

Query: 180 GEQWL 185
           G+ WL
Sbjct: 328 GKDWL 332

BLAST of Carg12862 vs. Swiss-Prot
Match: sp|Q9FM79|PME62_ARATH (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 8.5e-59
Identity = 113/238 (47.48%), Postives = 144/238 (60.50%), Query Frame = 0

Query: 3   DTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA--- 62
           DTVI+ + KASD    G ELGTYRTA+V+I+SD+FCA+ ITFENTVV+  G++GRQA   
Sbjct: 143 DTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQAVAL 202

Query: 63  ---------------------------------------------------QCVITSTAE 122
                                                               C I STA+
Sbjct: 203 RIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHSTAK 262

Query: 123 TFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGW 182
            +GAIAAHHR S  +DTGFSFV C I+G+G++ LGRAWG YSRT+YS C+I D+I+P+GW
Sbjct: 263 RYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVGW 322

Query: 183 SDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 187
           SDW  P RQR  +FG+Y CRG GA   G VPW++T T +EV+P++ R FI G+QWL L
Sbjct: 323 SDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLRL 380

BLAST of Carg12862 vs. Swiss-Prot
Match: sp|Q8VYZ3|PME53_ARATH (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.2e-38
Identity = 83/238 (34.87%), Postives = 119/238 (50.00%), Query Frame = 0

Query: 4   TVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTV-VSAPGDKGRQA--- 63
           T +     A   D  G  +GTY +A+ A++S +F A  ITF NT  V  PG  G+QA   
Sbjct: 146 TTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVAL 205

Query: 64  ---------------------------------------------------QCVITSTAE 123
                                                               C + + A+
Sbjct: 206 RVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIAD 265

Query: 124 TFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGW 183
             GA+ A  RSS  +DTGFSFV+C + G+G + LGRAWG +SR +++Y Y++++I P GW
Sbjct: 266 KLGAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGW 325

Query: 184 SDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 187
            +W DPSR+ T  +GQY+C G+GAN  G V WAR  T EE +P++   FI+G +W+ L
Sbjct: 326 YNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIKL 383

BLAST of Carg12862 vs. Swiss-Prot
Match: sp|Q8LPF3|PME68_ARATH (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 8.9e-32
Identity = 77/237 (32.49%), Postives = 112/237 (47.26%), Query Frame = 0

Query: 4   TVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTV-VSAPGDKGRQA--- 63
           T I  + +ASD   +G +L TY+TA+V + ++YF A  I+F NT     PG +G QA   
Sbjct: 121 TAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAF 180

Query: 64  ---------------------------------------------------QCVITSTAE 123
                                                               C + S A 
Sbjct: 181 RISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIAS 240

Query: 124 TFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGW 183
            FG+IAAH R+ P++ TGF+FV C + G+G + +GRA G YSR +Y+Y Y + +++  GW
Sbjct: 241 RFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGGW 300

Query: 184 SDWSDPS-RQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWL 185
            DW   S + +TA FG Y C G GA     V WAR    E   P++ ++F+NG  W+
Sbjct: 301 DDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWI 357

BLAST of Carg12862 vs. Swiss-Prot
Match: sp|Q9ZQA3|PME15_ARATH (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 5.9e-28
Identity = 50/120 (41.67%), Postives = 81/120 (67.50%), Query Frame = 0

Query: 65  STAETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMIS 124
           +T+   G+I A  R S D+ +GFSFV C I+GSG +LLGRAWG Y+  ++S  Y+  +I+
Sbjct: 266 NTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIIT 325

Query: 125 PLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWL 184
           P GW++W D ++++T  FG+++C G GA+ K  V + +  T  E   ++D +FI+G++WL
Sbjct: 326 PEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWL 385

BLAST of Carg12862 vs. Swiss-Prot
Match: sp|Q9SIJ9|PME11_ARATH (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.9e-26
Identity = 44/125 (35.20%), Postives = 79/125 (63.20%), Query Frame = 0

Query: 60  QCVITSTAETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYI 119
           +C + S +   G+I A  R+S  + +GF+F+ C + GSG   LGR WG YSR +++Y + 
Sbjct: 208 RCHLHSLSPNNGSITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFF 267

Query: 120 EDMISPLGWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFIN 179
            ++++P GW+ W D +++ T  +G+Y+C G GA+ +  V W++  + EE   ++ ++FI 
Sbjct: 268 SNVVAPQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIG 327

Query: 180 GEQWL 185
           G+ WL
Sbjct: 328 GKDWL 332

BLAST of Carg12862 vs. TrEMBL
Match: tr|A0A1S3CIB1|A0A1S3CIB1_CUCME (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103501278 PE=4 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 2.0e-81
Identity = 152/240 (63.33%), Postives = 172/240 (71.67%), Query Frame = 0

Query: 1   MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA- 60
           +T+TVIT N KASDK+++G+ELGTY+TATVAIDSDYFCA+GITFENT V+ PGDKGRQ  
Sbjct: 139 VTETVITWNDKASDKNINGVELGTYKTATVAIDSDYFCATGITFENTNVAKPGDKGRQGV 198

Query: 61  -----------------------------------------------------QCVITST 120
                                                                QCVITST
Sbjct: 199 ALRITGDKAVFYRVKFLGGQDTLLDDLGTHYFYQCHIRGSVDFIFGTARSLYEQCVITST 258

Query: 121 AETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPL 180
           AE++GAIAAHHR+SPDDDTGFSFVRCVINGSG+V LGRAWG YSRTIYS CYIED+I+PL
Sbjct: 259 AESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPL 318

Query: 181 GWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 187
           GWSDW+DPSRQRT VFGQY CRGSGANTK WVPWA+TF+ EEVRP+VDR +I GEQWLNL
Sbjct: 319 GWSDWNDPSRQRTVVFGQYNCRGSGANTKDWVPWAKTFSYEEVRPFVDRKYIKGEQWLNL 378

BLAST of Carg12862 vs. TrEMBL
Match: tr|A0A0A0K706|A0A0A0K706_CUCSA (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G160530 PE=4 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.0e-80
Identity = 152/240 (63.33%), Postives = 168/240 (70.00%), Query Frame = 0

Query: 1   MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA- 60
           +TDTVIT N KASDK + G+ELGTYRTATVAIDSDYFCA+GITFENTVV+ PGDKGRQ  
Sbjct: 131 VTDTVITWNDKASDKSIDGVELGTYRTATVAIDSDYFCATGITFENTVVAKPGDKGRQGV 190

Query: 61  -----------------------------------------------------QCVITST 120
                                                                QCVITST
Sbjct: 191 ALRITGDKAMFYRVKFLGGQDTLLDDLGTHYYYQCHIQGSVDFIFGTARSLYEQCVITST 250

Query: 121 AETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPL 180
           AE++GAIAAHHR+SPDDDTGFSFVRCVINGSG+V LGRAWG YSRTIYS CYIED+I+PL
Sbjct: 251 AESYGAIAAHHRASPDDDTGFSFVRCVINGSGKVYLGRAWGNYSRTIYSNCYIEDVINPL 310

Query: 181 GWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 187
           GWSDW+DPSRQRT  FGQY CRGSGA+TK WV WA+TF  EEVRP+VDR +I GEQWLNL
Sbjct: 311 GWSDWNDPSRQRTVAFGQYNCRGSGASTKDWVKWAKTFNYEEVRPFVDRKYIKGEQWLNL 370

BLAST of Carg12862 vs. TrEMBL
Match: tr|A0A067L452|A0A067L452_JATCU (Pectinesterase OS=Jatropha curcas OX=180498 GN=JCGZ_01017 PE=4 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.2e-64
Identity = 124/240 (51.67%), Postives = 154/240 (64.17%), Query Frame = 0

Query: 1   MTDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA- 60
           ++DT+I+ N+KASD D +G+ LGTYR+A+VAI+SDYFCA+GITFENTVV+ PG  G QA 
Sbjct: 100 ISDTIISWNNKASDIDSNGVALGTYRSASVAIESDYFCATGITFENTVVAVPGGYGMQAV 159

Query: 61  -----------------------------------------------------QCVITST 120
                                                                 CV+ S 
Sbjct: 160 ALRVSGDKAFFYRVKILGTQDTLLDETGSHYFYQCHIQGSVDFIFGRGKSLFEDCVLQSI 219

Query: 121 AETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPL 180
           A   GAIAAHHR  PDDD+GFSFV CVING+G++LLGRAWG YSR IYSYCYI+++I+P 
Sbjct: 220 ARRSGAIAAHHRDFPDDDSGFSFVNCVINGTGKILLGRAWGNYSRAIYSYCYIDNIITPS 279

Query: 181 GWSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 187
           GWSDW+ P RQ+T VFG+Y C G GA+T+G VPW++TFT EEVRP++D  FING QWL L
Sbjct: 280 GWSDWNYPDRQKTVVFGEYMCSGRGADTRGRVPWSKTFTYEEVRPFLDMQFINGHQWLRL 339

BLAST of Carg12862 vs. TrEMBL
Match: tr|B9S9Q8|B9S9Q8_RICCO (Pectinesterase OS=Ricinus communis OX=3988 GN=RCOM_0521220 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.5e-63
Identity = 122/238 (51.26%), Postives = 153/238 (64.29%), Query Frame = 0

Query: 3   DTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA--- 62
           DTVIT N+KASD D +G+ELGTYR+A+V I+SDYFCA+G+TFENTVV+ PG  G QA   
Sbjct: 146 DTVITWNNKASDMDSNGVELGTYRSASVTIESDYFCATGVTFENTVVAEPGGYGMQAVAL 205

Query: 63  ---------------------------------------------------QCVITSTAE 122
                                                               CV+ STA+
Sbjct: 206 RVSGDKAFFHKVRILGTQDTLLDETGSHFFYQCHIQGSVDFIFGKGRSLFQDCVLQSTAK 265

Query: 123 TFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLGW 182
             GAIAAHHR +P DD+GFSFV CVING+G++LLGRAWG YSR IYSYC+I+D+I+P GW
Sbjct: 266 RSGAIAAHHRDTPFDDSGFSFVGCVINGTGKILLGRAWGNYSRAIYSYCFIDDVITPPGW 325

Query: 183 SDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 187
           SDW+ P RQ+T  FG+Y C G GA+T G VPW++TF+ EEVRP++D  FING++WL L
Sbjct: 326 SDWNYPDRQKTVFFGEYECSGRGADTGGRVPWSKTFSYEEVRPFLDMQFINGDEWLRL 383

BLAST of Carg12862 vs. TrEMBL
Match: tr|A0A2I4DU44|A0A2I4DU44_9ROSI (Pectinesterase OS=Juglans regia OX=51240 GN=LOC108983471 PE=4 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.7e-62
Identity = 118/239 (49.37%), Postives = 157/239 (65.69%), Query Frame = 0

Query: 2   TDTVITSNSKASDKDVSGLELGTYRTATVAIDSDYFCASGITFENTVVSAPGDKGRQA-- 61
           +DT+IT N+KASDKD +G++LGTYR+A+VAI+SDYFCA+GITFENTV++ PG  G QA  
Sbjct: 122 SDTIITWNNKASDKDRNGVQLGTYRSASVAIESDYFCATGITFENTVIAVPGGYGMQAVA 181

Query: 62  ----------------------------------------------------QCVITSTA 121
                                                               +CVI S A
Sbjct: 182 LRIAGDKAVLYRVRILGTQDTLLDDTGSHYFYQCHIQGSVDFIFGTSRSLYQECVIESIA 241

Query: 122 ETFGAIAAHHRSSPDDDTGFSFVRCVINGSGRVLLGRAWGTYSRTIYSYCYIEDMISPLG 181
           + +GAIAAHHR SP+++TGFSFV C I G+G +LLGRAWG YSR +YSYC I+D+++  G
Sbjct: 242 KNWGAIAAHHRDSPNENTGFSFVNCNITGTGSILLGRAWGNYSRAVYSYCNIDDIVTSSG 301

Query: 182 WSDWSDPSRQRTAVFGQYRCRGSGANTKGWVPWARTFTSEEVRPYVDRNFINGEQWLNL 187
           WSDW  PSRQ+T VFG+Y+CRG GA+T+G V W+++F+ EEV+P++D+ FI+GEQWL L
Sbjct: 302 WSDWKHPSRQKTVVFGEYQCRGRGADTRGRVSWSKSFSYEEVQPFLDKKFISGEQWLRL 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022936222.13.6e-9877.50pectinesterase QRT1 [Cucurbita moschata][more]
XP_022975722.13.6e-9877.50pectinesterase QRT1 [Cucurbita maxima][more]
XP_023536329.11.4e-9468.63pectinesterase QRT1 [Cucurbita pepo subsp. pepo][more]
XP_008463039.13.0e-8163.33PREDICTED: pectinesterase QRT1 [Cucumis melo][more]
XP_004150299.11.5e-8063.33PREDICTED: pectinesterase QRT1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT5G55590.14.7e-6047.48Pectin lyase-like superfamily protein[more]
AT5G19730.11.2e-3934.87Pectin lyase-like superfamily protein[more]
AT5G47500.14.9e-3332.49Pectin lyase-like superfamily protein[more]
AT2G36710.13.3e-2941.67Pectin lyase-like superfamily protein[more]
AT2G21610.11.1e-2735.20pectinesterase 11[more]
Match NameE-valueIdentityDescription
sp|Q9FM79|PME62_ARATH8.5e-5947.48Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1[more]
sp|Q8VYZ3|PME53_ARATH2.2e-3834.87Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1[more]
sp|Q8LPF3|PME68_ARATH8.9e-3232.49Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1[more]
sp|Q9ZQA3|PME15_ARATH5.9e-2841.67Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1[more]
sp|Q9SIJ9|PME11_ARATH1.9e-2635.20Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3CIB1|A0A1S3CIB1_CUCME2.0e-8163.33Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103501278 PE=4 SV=1[more]
tr|A0A0A0K706|A0A0A0K706_CUCSA1.0e-8063.33Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G160530 PE=4 SV=1[more]
tr|A0A067L452|A0A067L452_JATCU2.2e-6451.67Pectinesterase OS=Jatropha curcas OX=180498 GN=JCGZ_01017 PE=4 SV=1[more]
tr|B9S9Q8|B9S9Q8_RICCO2.5e-6351.26Pectinesterase OS=Ricinus communis OX=3988 GN=RCOM_0521220 PE=4 SV=1[more]
tr|A0A2I4DU44|A0A2I4DU44_9ROSI2.7e-6249.37Pectinesterase OS=Juglans regia OX=51240 GN=LOC108983471 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: INTERPRO
TermDefinition
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
IPR000070Pectinesterase_cat
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12862-RACarg12862-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 67..180
e-value: 9.0E-20
score: 70.5
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 64..186
e-value: 6.8E-32
score: 113.0
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 1..63
e-value: 4.0E-8
score: 35.1
NoneNo IPR availablePANTHERPTHR31321FAMILY NOT NAMEDcoord: 2..59
NoneNo IPR availablePANTHERPTHR31321:SF31PECTINESTERASE QRT1coord: 2..59
NoneNo IPR availablePANTHERPTHR31321:SF31PECTINESTERASE QRT1coord: 60..186
NoneNo IPR availablePANTHERPTHR31321FAMILY NOT NAMEDcoord: 60..186
IPR011050Pectin lyase fold/virulence factorSUPERFAMILYSSF51126Pectin lyase-likecoord: 2..185