Carg12736 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg12736
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Cytochrome P450
Location	Cucurbita_argyrosperma_scaffold_021 : 1084409 .. 1085452 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAATTTCCAAAGCTACACAATTCTCTTCCTCATATGGCTACTTTCCACCATCACAATCCGCTTCATTTTTGCCAAAACCAAGCCAAACCCTCGCCTTCCTCCAACCCCTTTAGCCCTTCCCATCATCGGCCATCTCCACCTCCTAGGACCCCTACCCCACAAAGCTTTCCACAAGCTCTCGACCCGCCATGGCCCTCTAATTCACCTCCGTCTAGGCTCCGTCCCTTGCATCGTAGTTTCATCCGCCGAAATGGCAAAACAAGTCCTCAAAACACACGAATCGTCCTTCTCAAACCGACCCCATTTGGCTGCAGTTGACTATCTAACCTACGGCTCGGCGGACTTCTCCTTTGCTCCCTATGGACGCTATTGGAAGTTCATGAAGAAGCTTTGTATGTCGGAGCTCCTCGGTAGCCGAACGCTCGACAGCCTTGCTCCGATAAGACAAGACGAGACACGGCGGTTTCTACGGTCATTACAGACGAAAGCGGTCGAGGGGAAAGATGTTGATGTTGGAAGAGAGTTGATGAGGGCGAGCAACAATGTGATATCGAGAATGACGTTGGGGAAACGAAGCGCGGAGGAGGAAGAAGAAGCGGAGGGGATAAGGATGTTGGTTAAGGATGTAGCTGAGCTTACTGGGACTTTTAATGTTGCGGATTACATTTGGTTTTGTAAGGGTTTGGACTTGCAAGGGCTTGGGAAGAGGTTGAGGGAAGTTCGTGAGAGGTTTGATGAGATGATGGAGAGGTTGATAATGGAGCATGAAGAGAGCTTGAAGATGATGGAAATGGAGGGAAATGGTGATGAGAAAGTGATGGATTTGCTTCATATTTTGCTTCTTAAGTTTAGAGATGAAAACTCGGAAGTGAAATTGACTCGAGAGAATATCAAGGCTTTTATTTTGGTAAGTTCGATTTAAAGTTATGGTTTAGTTCTCGATGTTAGAGTCACGGACCTCCATAACGGTATGATATTGTTCACTTACTTATAGACCCATGATCATCCCCTTAGTTATCCGATATGGGACTCCCACTAA

mRNA sequence

ATGGAGAATTTCCAAAGCTACACAATTCTCTTCCTCATATGGCTACTTTCCACCATCACAATCCGCTTCATTTTTGCCAAAACCAAGCCAAACCCTCGCCTTCCTCCAACCCCTTTAGCCCTTCCCATCATCGGCCATCTCCACCTCCTAGGACCCCTACCCCACAAAGCTTTCCACAAGCTCTCGACCCGCCATGGCCCTCTAATTCACCTCCGTCTAGGCTCCGTCCCTTGCATCGTAGTTTCATCCGCCGAAATGGCAAAACAAGTCCTCAAAACACACGAATCGTCCTTCTCAAACCGACCCCATTTGGCTGCAGTTGACTATCTAACCTACGGCTCGGCGGACTTCTCCTTTGCTCCCTATGGACGCTATTGGAAGTTCATGAAGAAGCTTTGTATGTCGGAGCTCCTCGGTAGCCGAACGCTCGACAGCCTTGCTCCGATAAGACAAGACGAGACACGGCGGTTTCTACGGTCATTACAGACGAAAGCGGTCGAGGGGAAAGATGTTGATGTTGGAAGAGAGTTGATGAGGGCGAGCAACAATGTGATATCGAGAATGACGTTGGGGAAACGAAGCGCGGAGGAGGAAGAAGAAGCGGAGGGGATAAGGATGTTGGTTAAGGATGTAGCTGAGCTTACTGGGACTTTTAATGTTGCGGATTACATTTGGTTTTGTAAGGGTTTGGACTTGCAAGGGCTTGGGAAGAGGTTGAGGGAAGTTCGTGAGAGGTTTGATGAGATGATGGAGAGGTTGATAATGGAGCATGAAGAGAGCTTGAAGATGATGGAAATGGAGGGAAATGGTGATGAGAAAGTGATGGATTTGCTTCATATTTTGCTTCTTAAGTTTAGAGATGAAAACTCGGAAGTGAAATTGACTCGAGAGAATATCAAGGCTTTTATTTTGACCCATGATCATCCCCTTAGTTATCCGATATGGGACTCCCACTAA

Coding sequence (CDS)

ATGGAGAATTTCCAAAGCTACACAATTCTCTTCCTCATATGGCTACTTTCCACCATCACAATCCGCTTCATTTTTGCCAAAACCAAGCCAAACCCTCGCCTTCCTCCAACCCCTTTAGCCCTTCCCATCATCGGCCATCTCCACCTCCTAGGACCCCTACCCCACAAAGCTTTCCACAAGCTCTCGACCCGCCATGGCCCTCTAATTCACCTCCGTCTAGGCTCCGTCCCTTGCATCGTAGTTTCATCCGCCGAAATGGCAAAACAAGTCCTCAAAACACACGAATCGTCCTTCTCAAACCGACCCCATTTGGCTGCAGTTGACTATCTAACCTACGGCTCGGCGGACTTCTCCTTTGCTCCCTATGGACGCTATTGGAAGTTCATGAAGAAGCTTTGTATGTCGGAGCTCCTCGGTAGCCGAACGCTCGACAGCCTTGCTCCGATAAGACAAGACGAGACACGGCGGTTTCTACGGTCATTACAGACGAAAGCGGTCGAGGGGAAAGATGTTGATGTTGGAAGAGAGTTGATGAGGGCGAGCAACAATGTGATATCGAGAATGACGTTGGGGAAACGAAGCGCGGAGGAGGAAGAAGAAGCGGAGGGGATAAGGATGTTGGTTAAGGATGTAGCTGAGCTTACTGGGACTTTTAATGTTGCGGATTACATTTGGTTTTGTAAGGGTTTGGACTTGCAAGGGCTTGGGAAGAGGTTGAGGGAAGTTCGTGAGAGGTTTGATGAGATGATGGAGAGGTTGATAATGGAGCATGAAGAGAGCTTGAAGATGATGGAAATGGAGGGAAATGGTGATGAGAAAGTGATGGATTTGCTTCATATTTTGCTTCTTAAGTTTAGAGATGAAAACTCGGAAGTGAAATTGACTCGAGAGAATATCAAGGCTTTTATTTTGACCCATGATCATCCCCTTAGTTATCCGATATGGGACTCCCACTAA

Protein sequence

MENFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHKLSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFAPYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRASNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLREVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIKAFILTHDHPLSYPIWDSH

Homology

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BLAST of Carg12736 vs. NCBI nr
Match: XP_023518782.1 (cytochrome P450 93A2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592.8 bits (1527), Expect = 6.9e-166
Identity = 301/304 (99.01%), Postives = 303/304 (99.67%), Query Frame = 0


Query: 1   MENFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60
           ME+FQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK
Sbjct: 1   MEDFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120
           LSTRHGPL+HLRLGSVPCIVVSSAE AKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA
Sbjct: 61  LSTRHGPLMHLRLGSVPCIVVSSAETAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120

Query: 121 PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRA 180
           PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRA
Sbjct: 121 PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRA 180

Query: 181 SNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLR 240
           SNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLR
Sbjct: 181 SNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLR 240

Query: 241 EVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK 300
           EVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK
Sbjct: 241 EVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK 300

Query: 301 AFIL 305
           AFIL
Sbjct: 301 AFIL 304

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BLAST of Carg12736 vs. NCBI nr
Match: XP_022925132.1 (cytochrome P450 93A2 [Cucurbita moschata])

HSP 1 Score: 592.4 bits (1526), Expect = 9.0e-166
Identity = 300/304 (98.68%), Postives = 303/304 (99.67%), Query Frame = 0


Query: 1   MENFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60
           ME+FQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK
Sbjct: 1   MEDFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120
           LSTRHGPLIHLRLGSVPCI+VSSAE AKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA
Sbjct: 61  LSTRHGPLIHLRLGSVPCIIVSSAETAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120

Query: 121 PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRA 180
           PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRA
Sbjct: 121 PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRA 180

Query: 181 SNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLR 240
           SNNVISRMTLGKRSAEEEEEAEGIR+LVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLR
Sbjct: 181 SNNVISRMTLGKRSAEEEEEAEGIRLLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLR 240

Query: 241 EVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK 300
           EVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK
Sbjct: 241 EVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK 300

Query: 301 AFIL 305
           AFIL
Sbjct: 301 AFIL 304

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BLAST of Carg12736 vs. NCBI nr
Match: XP_022966275.1 (cytochrome P450 93A2 [Cucurbita maxima])

HSP 1 Score: 582.0 bits (1499), Expect = 1.2e-162
Identity = 295/304 (97.04%), Postives = 300/304 (98.68%), Query Frame = 0


Query: 1   MENFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60
           ME+FQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK
Sbjct: 1   MEDFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120
           LS RHGPLIHLRLGSVPCIVVSSAE AKQVLKTHESSFSNRPHLAAVDYLTY SADFSFA
Sbjct: 61  LSARHGPLIHLRLGSVPCIVVSSAETAKQVLKTHESSFSNRPHLAAVDYLTYNSADFSFA 120

Query: 121 PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRA 180
           PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDET+RFLRSLQTKAVEGKDVDVGRELMRA
Sbjct: 121 PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETQRFLRSLQTKAVEGKDVDVGRELMRA 180

Query: 181 SNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLR 240
           SNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNV+DYIWFCKGLDLQGLGK+LR
Sbjct: 181 SNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVSDYIWFCKGLDLQGLGKKLR 240

Query: 241 EVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK 300
           EVRERFD+MMERLI EHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK
Sbjct: 241 EVRERFDKMMERLIKEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK 300

Query: 301 AFIL 305
           AFIL
Sbjct: 301 AFIL 304

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BLAST of Carg12736 vs. NCBI nr
Match: XP_004150165.1 (PREDICTED: cytochrome P450 93A2-like [Cucumis sativus])

HSP 1 Score: 481.9 bits (1239), Expect = 1.7e-132
Identity = 245/304 (80.59%), Postives = 265/304 (87.17%), Query Frame = 0


Query: 1   MENFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60
           ME+F+SY ILF IWL+ST+TIR IFAK K N  LPP+P ALPIIGHLHLLGPLPHKAFHK
Sbjct: 1   MEDFESYIILFFIWLVSTLTIRLIFAKNKHNSHLPPSPFALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120
           LS RHGPL+HLRLGSVPC+VVSS E AKQVLKT ESSFSNRPHL+AVDYLTYGSADFSFA
Sbjct: 61  LSNRHGPLMHLRLGSVPCVVVSSPETAKQVLKTQESSFSNRPHLSAVDYLTYGSADFSFA 120

Query: 121 PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRA 180
           PYG YWKFMKKLCMSELLG RTLDSL P+R+DE RRFLRSLQ+KA +GK+VDVG ELMR 
Sbjct: 121 PYGPYWKFMKKLCMSELLGGRTLDSLRPMREDEIRRFLRSLQSKAADGKEVDVGGELMRL 180

Query: 181 SNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLR 240
           SNNVISRMTLGKR AEEEEEAEGIR LVKD+A LTGTFNV+DYIWFCK LDLQ LGKRL+
Sbjct: 181 SNNVISRMTLGKRCAEEEEEAEGIRKLVKDIAVLTGTFNVSDYIWFCKNLDLQRLGKRLK 240

Query: 241 EVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK 300
           EVRERFDEMMER+I EHE++          D KV DLL ILLLKF DENSEVKLTRENIK
Sbjct: 241 EVRERFDEMMERMIKEHEDNC--------SDGKVKDLLDILLLKFGDENSEVKLTRENIK 296

Query: 301 AFIL 305
           AF+L
Sbjct: 301 AFVL 296

[Close]

BLAST of Carg12736 vs. NCBI nr
Match: KGN53825.1 (hypothetical protein Csa_4G152790 [Cucumis sativus])

HSP 1 Score: 481.9 bits (1239), Expect = 1.7e-132
Identity = 245/306 (80.07%), Postives = 266/306 (86.93%), Query Frame = 0


Query: 1   MENFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60
           ME+F+SY ILF IWL+ST+TIR IFAK K N  LPP+P ALPIIGHLHLLGPLPHKAFHK
Sbjct: 1   MEDFESYIILFFIWLVSTLTIRLIFAKNKHNSHLPPSPFALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120
           LS RHGPL+HLRLGSVPC+VVSS E AKQVLKT ESSFSNRPHL+AVDYLTYGSADFSFA
Sbjct: 61  LSNRHGPLMHLRLGSVPCVVVSSPETAKQVLKTQESSFSNRPHLSAVDYLTYGSADFSFA 120

Query: 121 PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRA 180
           PYG YWKFMKKLCMSELLG RTLDSL P+R+DE RRFLRSLQ+KA +GK+VDVG ELMR 
Sbjct: 121 PYGPYWKFMKKLCMSELLGGRTLDSLRPMREDEIRRFLRSLQSKAADGKEVDVGGELMRL 180

Query: 181 SNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLR 240
           SNNVISRMTLGKR AEEEEEAEGIR LVKD+A LTGTFNV+DYIWFCK LDLQ LGKRL+
Sbjct: 181 SNNVISRMTLGKRCAEEEEEAEGIRKLVKDIAVLTGTFNVSDYIWFCKNLDLQRLGKRLK 240

Query: 241 EVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK 300
           EVRERFDEMMER+I EHE++          D KV DLL ILLLKF DENSEVKLTRENIK
Sbjct: 241 EVRERFDEMMERMIKEHEDNC--------SDGKVKDLLDILLLKFGDENSEVKLTRENIK 298

Query: 301 AFILTH 307
           AF+L +
Sbjct: 301 AFVLVN 298

[Close]

BLAST of Carg12736 vs. TAIR10
Match: AT5G06900.1 (cytochrome P450, family 93, subfamily D, polypeptide 1)

HSP 1 Score: 274.2 bits (700), Expect = 9.8e-74
Identity = 142/306 (46.41%), Postives = 211/306 (68.95%), Query Frame = 0


Query: 1   MENFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60
           M + Q ++++ L+ L  T+ I+ I  + +    LPP+P ALPIIGH+HLLGP+ H+A HK
Sbjct: 1   MVDLQYFSVIILVCLGITVLIQAITNRLRDRLPLPPSPTALPIIGHIHLLGPIAHQALHK 60

Query: 61  LSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120
           LS R+GPL++L +GS+P ++VSSAEMA ++LK++E +F NRP +  VDYLTYGSADF  A
Sbjct: 61  LSIRYGPLMYLFIGSIPNLIVSSAEMANEILKSNELNFLNRPTMQNVDYLTYGSADFFSA 120

Query: 121 PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRA 180
           PYG +WKFMK++CM EL  SR LDS   +R +E ++ L  +  KA   + V++G +L   
Sbjct: 121 PYGLHWKFMKRICMVELFSSRALDSFVSVRSEELKKLLIRVLKKAEAEESVNLGEQLKEL 180

Query: 181 SNNVISRMTLGKRSAEEE--EEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKR 240
           ++N+I+RM   K  ++ +  E++E +  +V ++ EL G FNV++  WF K LDLQGL KR
Sbjct: 181 TSNIITRMMFRKMQSDSDGGEKSEEVIKMVVELNELAGFFNVSETFWFLKRLDLQGLKKR 240

Query: 241 LREVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTREN 300
           L+  R+++D ++ER++ EHE S K    E N       +L +LL  + D+N+E+KLTREN
Sbjct: 241 LKNARDKYDVIIERIMEEHESSKKNATGERN-------MLDVLLDIYEDKNAEMKLTREN 299

Query: 301 IKAFIL 305
           IKAFI+
Sbjct: 301 IKAFIM 299

[Close]

BLAST of Carg12736 vs. TAIR10
Match: AT2G42250.1 (cytochrome P450, family 712, subfamily A, polypeptide 1)

HSP 1 Score: 203.8 bits (517), Expect = 1.6e-52
Identity = 109/272 (40.07%), Postives = 169/272 (62.13%), Query Frame = 0


Query: 33  RLPPTPLALPIIGHLHLLGPLPHKAFHKLSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLK 92
           +LP +P ALP IGHLHL+G +   +F  L+ ++GPL+ +RLG+  C+VVSS+ +A+++ K
Sbjct: 40  KLPQSPPALPFIGHLHLIGKVLPVSFQSLAHKYGPLMEIRLGASKCVVVSSSSVAREIFK 99

Query: 93  THESSFSNRPHLAAVDYLTYGSADFSFAPYGRYWKFMKKLCMSELLGSRTLDSLAPIRQD 152
             E +FS+RP   + +Y  Y  + F  A YG YW+FMKKLCM++LL    L+  A IR++
Sbjct: 100 EQELNFSSRPEFGSAEYFKYRGSRFVLAQYGDYWRFMKKLCMTKLLAVPQLEKFADIREE 159

Query: 153 ETRRFLRSLQTKAVEGKDVDVGRELMRASNNVISRMTLGKRSAEEEEEAEGIRMLVKDVA 212
           E  + + S+     EG   D+  + ++ +NNVI RM +  R +  + EAE IR LVK   
Sbjct: 160 EKLKLVDSVAKCCREGLPCDLSSQFIKYTNNVICRMAMSTRCSGTDNEAEEIRELVKKSL 219

Query: 213 ELTGTFNVADYIWFCKGLDLQGLGKRLREVRERFDEMMERLIMEHEESLKMMEMEGNGDE 272
           EL G  +V D +   K +D  G GK+L  V E++D ++ER++ E E   K    + +G  
Sbjct: 220 ELAGKISVGDVLGPLKVMDFSGNGKKLVAVMEKYDLLVERIMKEREAKAK----KKDGTR 279

Query: 273 KVMDLLHILLLKFRDENSEVKLTRENIKAFIL 305
           K  D+L ILL  +RD  +E+K+TR ++K+F+L
Sbjct: 280 K--DILDILLETYRDPTAEMKITRNDMKSFLL 305

[Close]

BLAST of Carg12736 vs. TAIR10
Match: AT1G50560.1 (cytochrome P450, family 705, subfamily A, polypeptide 25)

HSP 1 Score: 200.3 bits (508), Expect = 1.8e-51
Identity = 120/312 (38.46%), Postives = 185/312 (59.29%), Query Frame = 0


Query: 3   NFQSYTILFLIWLLSTITIRFIFAKTKPNPR-----LPPTPLALPIIGHLHLLGPLP-HK 62
           +FQ   I  L+ + S       F K   +P+     LPP+P +LPIIGHLH L  +P +K
Sbjct: 7   DFQLCFIFILLAIFSLFCFSAFFFKKPKDPQLQGCGLPPSPPSLPIIGHLHFLLSVPCYK 66

Query: 63  AFHKLSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPH---LAAVDYLTYG 122
           +F KLS+++GP +HLR  ++P ++VSS  MA +VL+  + +F++R           L +G
Sbjct: 67  SFQKLSSKYGPFLHLRAFNIPIVLVSSGSMANEVLRIQDLNFASRDSGQTPIMEKSLLFG 126

Query: 123 SADFSFAPYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDV 182
           S  F   PYG YW+FMKKL + +LLGS +L+    +R  E + F   L  KA + + VDV
Sbjct: 127 SFGFVSVPYGDYWRFMKKLLVKKLLGSHSLEQTRLLRGKELQTFRAMLFDKAAKNETVDV 186

Query: 183 GRELMRASNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYI-WFCKGLDL 242
           G+E+M+ +NN I RMT+G+  +EE  EAE +R LV     LT  F +A  +  F K + +
Sbjct: 187 GKEMMKLTNNSICRMTMGRSCSEENGEAEQVRGLVTKSLSLTKKFLIASIVGQFSKLVGI 246

Query: 243 QGLGKRLREVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEV 302
              GK + EV +R+DE++E++I EHEE+        NG+++  D++ +LL    D+N+E 
Sbjct: 247 SLFGKEIMEVSQRYDELLEKIIKEHEEN------PNNGEDR--DMMDVLLEVCADDNAEF 306

Query: 303 KLTRENIKAFIL 305
           K++R  IKA  +
Sbjct: 307 KISRNQIKALFV 310

[Close]

BLAST of Carg12736 vs. TAIR10
Match: AT2G05180.1 (cytochrome P450, family 705, subfamily A, polypeptide 6)

HSP 1 Score: 196.4 bits (498), Expect = 2.6e-50
Identity = 122/307 (39.74%), Postives = 184/307 (59.93%), Query Frame = 0


Query: 3   NFQSYTILFLIWLLSTITIRFIFAKTKPNPR---LPPTPLALPIIGHLHLL-GPLPHKAF 62
           +FQ+  I  ++ L S       F K K +PR   LPP+P +LPIIGHLHLL  PL HK+ 
Sbjct: 8   DFQNCFIFIVLCLFSLFCYSLFFKKPK-HPRGCDLPPSPPSLPIIGHLHLLFSPLTHKSL 67

Query: 63  HKLSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFS 122
            KLS+++G L+HLR+ +VP I+VSSA +A ++ + H+ + S+R   A  + L +GS+   
Sbjct: 68  QKLSSKYGHLLHLRIFNVPVILVSSASVAYEIFRAHDVNVSSRGVAAIDESLLFGSSGII 127

Query: 123 FAPYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELM 182
            APYG YWKFMKKL  ++LL  ++L+S   IR +E  RF  SL  KA   + V++  E M
Sbjct: 128 TAPYGDYWKFMKKLIATKLLRPQSLESSRGIRGEELTRFYNSLLDKARMTESVEISTEAM 187

Query: 183 RASNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWF-CKGLDLQGLGK 242
           +  NN + RM++G+  +EE  EAE IR LV +   LT    +A  +    K L +    K
Sbjct: 188 KLVNNTLCRMSMGRSFSEENGEAEKIRGLVGESYALTKKMFLASLLRKPLKKLRISLFEK 247

Query: 243 RLREVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRE 302
            +  V +R DE++ER+++EHEE L       + + +  D++ +LL    DEN+E  +TR 
Sbjct: 248 EIMGVSDRLDELLERILVEHEEKL-------HEEHQGTDMMDVLLAASGDENAEYNITRN 306

Query: 303 NIKAFIL 305
           +IK+F +
Sbjct: 308 HIKSFFV 306

[Close]

BLAST of Carg12736 vs. TAIR10
Match: AT1G50520.1 (cytochrome P450, family 705, subfamily A, polypeptide 27)

HSP 1 Score: 195.3 bits (495), Expect = 5.8e-50
Identity = 119/311 (38.26%), Postives = 180/311 (57.88%), Query Frame = 0


Query: 3   NFQSYTILFLIWLLSTITIRFIFAKTKPNPR-----LPPTPLALPIIGHLHLLGPLP-HK 62
           +FQ   I  L+ + S   +   F K   +P+     LPP+P +LP+IGHLHLL  +P  K
Sbjct: 7   DFQLCFIFILLGIFSLFCLSAFFFKKPKDPQLQGCGLPPSPPSLPVIGHLHLLLSVPCLK 66

Query: 63  AFHKLSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAV--DYLTYGS 122
           +F KLS+++GPL+HLR  ++P ++VSS  MA +VL+T + +F+ R    ++    L +GS
Sbjct: 67  SFQKLSSKYGPLLHLRAFNIPIVIVSSGSMANEVLRTQDLNFATRQREVSIMEKSLLFGS 126

Query: 123 ADFSFAPYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVG 182
             F  APYG YW+FMKKL ++ L GS +L+    IR+ E + F   L  KA +   VDVG
Sbjct: 127 FGFVSAPYGDYWRFMKKLLVTNLFGSHSLEQTRLIREKELKTFRTMLFDKAAKKGTVDVG 186

Query: 183 RELMRASNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIW-FCKGLDLQ 242
           +E+M+ +NN I RM +G+R +EE  EAE +  LV     L     +A+ +    K   + 
Sbjct: 187 KEMMKLTNNSICRMIMGRRCSEENSEAEKVEDLVIKSFSLVKKVLIANTVGRLLKKFGIS 246

Query: 243 GLGKRLREVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVK 302
              K + EV +R+DE++E++I EHEE     E          D++ +LL    D+ +EVK
Sbjct: 247 LFEKEIMEVSQRYDELLEKIIKEHEEDPNKKEDR--------DMMDVLLEVCADDKAEVK 306

Query: 303 LTRENIKAFIL 305
           +TR  IKA I+
Sbjct: 307 ITRNQIKALIV 309

[Close]

BLAST of Carg12736 vs. Swiss-Prot
Match: sp|Q42798|C93A1_SOYBN (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 3.9e-80
Identity = 153/302 (50.66%), Postives = 215/302 (71.19%), Query Frame = 0


Query: 6   SYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHKLSTRH 65
           +Y +L LI L+STI   +I  + +    LPP+P ALPIIGHLHL+ P+PH+ F+KLSTRH
Sbjct: 2   AYQVL-LICLVSTIVFAYILWRKQSKKNLPPSPKALPIIGHLHLVSPIPHQDFYKLSTRH 61

Query: 66  GPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRP-HLAAVDYLTYGSAD--FSFAPY 125
           GP++ L LGSVPC+V S+AE AK+ LKTHE +FSNRP    AV  L Y S D  F+FAP+
Sbjct: 62  GPIMQLFLGSVPCVVASTAEAAKEFLKTHEINFSNRPGQNVAVKGLAYDSQDFLFAFAPF 121

Query: 126 GRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRASN 185
           G YWKFMKKLCMSELL  R +D   P+RQ ET+RF+  +  K V G+ VD G ELM  SN
Sbjct: 122 GPYWKFMKKLCMSELLSGRMMDQFLPVRQQETKRFISRVFRKGVAGEAVDFGDELMTLSN 181

Query: 186 NVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLREV 245
           N++SRMTL ++++E + +AE ++ LV ++AEL G FNV+D+IW+ K  DLQG  ++++E 
Sbjct: 182 NIVSRMTLSQKTSENDNQAEEMKKLVSNIAELMGKFNVSDFIWYLKPFDLQGFNRKIKET 241

Query: 246 RERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIKAF 305
           R+RFD +++ +I + +E  +  +  G   ++  D+L +LL    DEN+E+KL ++NIKAF
Sbjct: 242 RDRFDVVVDGIIKQRQEERRKNKETGTA-KQFKDMLDVLLDMHEDENAEIKLDKKNIKAF 301

[Close]

BLAST of Carg12736 vs. Swiss-Prot
Match: sp|O81973|C93A3_SOYBN (Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 3.9e-80
Identity = 159/303 (52.48%), Postives = 211/303 (69.64%), Query Frame = 0


Query: 6   SYTILFLIWLLSTITIRFIF--AKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHKLST 65
           ++ +LF I L+STI    I    + K    LPP+P+ LPIIGHLHLL P PH+ FHKLS 
Sbjct: 2   AFQVLF-ICLISTIVFASILWRKQNKNKTLLPPSPMPLPIIGHLHLLSPTPHQDFHKLSL 61

Query: 66  RHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRP-HLAAVDYLTYGSADFSFAPY 125
           R+GP+IHL LGSVPC+V S+AE AK+ LKTHE +FSNRP +  AV+ LTYG  DF FAPY
Sbjct: 62  RYGPIIHLFLGSVPCVVASTAEAAKEFLKTHEPAFSNRPANTVAVETLTYGFQDFLFAPY 121

Query: 126 GRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRASN 185
           G YWKFMKKLCMSELLG   LD   P+RQ ET++F++ +  K + G+ VD G E +  SN
Sbjct: 122 GPYWKFMKKLCMSELLGGHMLDQFLPVRQXETKKFIKRVLQKGISGEAVDFGGEFITLSN 181

Query: 186 NVISRMTLGKRS-AEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLRE 245
           N++SRM + + S  E+E E E +R LVKD AEL+G FN++D++ F K  DLQG  KRL +
Sbjct: 182 NIVSRMIVSQTSTTEDENEVEEMRKLVKDAAELSGKFNISDFVSFLKRFDLQGFNKRLEK 241

Query: 246 VRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIKA 305
           +R+ FD +++R+I + EE  +  + E  G  +  D+L +L     DE+SE+KL +ENIKA
Sbjct: 242 IRDCFDTVLDRIIKQREEE-RRNKNETVGKREFKDMLDVLFDISEDESSEIKLNKENIKA 301

[Close]

BLAST of Carg12736 vs. Swiss-Prot
Match: sp|Q42799|C93A2_SOYBN (Cytochrome P450 93A2 OS=Glycine max OX=3847 GN=CYP93A2 PE=2 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 6.7e-80
Identity = 157/300 (52.33%), Postives = 214/300 (71.33%), Query Frame = 0


Query: 6   SYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHKLSTRH 65
           +Y +L +I ++S+I   +I  + +   +LPP+P  LPIIGHLHL+ P+PH+ F+KLS RH
Sbjct: 2   AYQVL-VICVVSSIVFAYIVWRKERKKKLPPSPKGLPIIGHLHLVSPIPHQDFYKLSLRH 61

Query: 66  GPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRP-HLAAVDYLTYGSADFSFAPYGR 125
           GP++ L LGSVPC+V S+AE AK+ LKTHE +FSNRP    AV +LTY      F PYG 
Sbjct: 62  GPIMQLFLGSVPCVVASTAEAAKEFLKTHEINFSNRPGQNVAVQFLTY-----VFGPYGP 121

Query: 126 YWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRASNNV 185
             KF+KKLCMSELLG R LD   P+RQ ET++F++ +  K + G+ VD G E MR SNN+
Sbjct: 122 SVKFIKKLCMSELLGGRMLDQFLPVRQQETKKFIKRVLQKGIAGEAVDFGGEFMRLSNNI 181

Query: 186 ISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLREVRE 245
           ISRMT+ + S+E+E++AE +RMLV DVAEL GTFNV+D+IWF K  DLQG  KR+R+ R 
Sbjct: 182 ISRMTMNQTSSEDEKQAEEMRMLVADVAELMGTFNVSDFIWFLKPFDLQGFNKRIRKTRI 241

Query: 246 RFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIKAFIL 305
           RFD +++R+I + EE  +    E  G  +  D+L +LL    D++SE+KLT+ENIKAFI+
Sbjct: 242 RFDAVLDRIIKQREEE-RRNNKEIGGTRQFKDILDVLLDIGEDDSSEIKLTKENIKAFIM 294

[Close]

BLAST of Carg12736 vs. Swiss-Prot
Match: sp|Q5VRI5|C93G2_ORYSJ (Cytochrome P450 93G2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP93G2 PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 3.2e-66
Identity = 135/280 (48.21%), Postives = 182/280 (65.00%), Query Frame = 0


Query: 26  AKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHKLSTRHGPLIHLRLG-SVPCIVVSSA 85
           +++    RLPP+P+ALPIIGHLHL+ P PH+AF ++  RHGPL++LRLG S  C+V+ SA
Sbjct: 31  SRSSKRGRLPPSPMALPIIGHLHLIRPPPHRAFDRILARHGPLVYLRLGPSTHCVVIGSA 90

Query: 86  EMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFAPYGRYWKFMKKLCMSELLGSRTLD 145
           ++A+  LK  E+S   RP  A    L YG A F+FAPYG YW+F+K+LCMSELLG RT++
Sbjct: 91  DVARDFLK-FEASIPERPPTAVTRQLAYGKAGFAFAPYGAYWRFVKRLCMSELLGPRTVE 150

Query: 146 SLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRASNNVISRMTLGKRSAEEEEEAEGI 205
            L P+R  E    LR+ Q+ A  G+ VD+  EL+R +NN I RM     SA   E AE  
Sbjct: 151 LLRPVRAAELADVLRAAQSAAERGEGVDMSHELVRMANNSIMRMV---ASALPGEMAEAA 210

Query: 206 RMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLREVRERFDEMMERLIMEHEESLKMM 265
           R   K VAEL G FN  D++  C+G DLQG+G+R  EV  RFD ++E +I   EE+ +  
Sbjct: 211 RDCAKQVAELVGAFNAEDFVAVCRGWDLQGIGRRTNEVHARFDALLETIIEAKEEARRRS 270

Query: 266 EMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIKAFIL 305
              G  +    DLL +L+    D+ +EVKLTR+NIKAF+L
Sbjct: 271 LRLGRRESSSKDLLDMLMDAAEDDTAEVKLTRDNIKAFVL 306

[Close]

BLAST of Carg12736 vs. Swiss-Prot
Match: sp|Q9XHC6|C93E1_SOYBN (Beta-amyrin 24-hydroxylase OS=Glycine max OX=3847 GN=CYP93E1 PE=1 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 7.2e-66
Identity = 137/306 (44.77%), Postives = 197/306 (64.38%), Query Frame = 0


Query: 1   MENFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPP-TPLALPIIGHLHLLGPLPHKAFH 60
           M + + Y +LF +W +STI IR IF K +   RLPP  P+++P++GH   L  L H+A +
Sbjct: 1   MLDIKGYLVLFFLWFISTILIRSIFKKPQ-RLRLPPGPPISIPLLGHAPYLRSLLHQALY 60

Query: 61  KLSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSF 120
           KLS R+GPLIH+ +GS   +V SSAE AKQ+LKT E +F NRP + A + LTYG+AD+ F
Sbjct: 61  KLSLRYGPLIHVMIGSKHVVVASSAETAKQILKTSEEAFCNRPLMIASESLTYGAADYFF 120

Query: 121 APYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGK-DVDVGRELM 180
            PYG YW+F+KKLCM+ELL  +TL+    IR+ E   FL+ +   +  G  +V + +EL+
Sbjct: 121 IPYGTYWRFLKKLCMTELLSGKTLEHFVRIRESEVEAFLKRMMEISGNGNYEVVMRKELI 180

Query: 181 RASNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKR 240
             +NN+I+RM +GK+S  E +E   +R +V++V EL G FN+ D I F + LDLQG GK+
Sbjct: 181 THTNNIITRMIMGKKSNAENDEVARLRKVVREVGELLGAFNLGDVIGFMRPLDLQGFGKK 240

Query: 241 LREVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTREN 300
             E   + D MME+++ EHEE+      E    ++  DL  ILL     + ++ KLTRE+
Sbjct: 241 NMETHHKVDAMMEKVLREHEEA---RAKEDADSDRKKDLFDILLNLIEADGADNKLTRES 300

Query: 301 IKAFIL 305
            KAF L
Sbjct: 301 AKAFAL 302

[Close]

BLAST of Carg12736 vs. TrEMBL
Match: tr|A0A0A0KW21|A0A0A0KW21_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G152790 PE=4 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 1.1e-132
Identity = 245/306 (80.07%), Postives = 266/306 (86.93%), Query Frame = 0


Query: 1   MENFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60
           ME+F+SY ILF IWL+ST+TIR IFAK K N  LPP+P ALPIIGHLHLLGPLPHKAFHK
Sbjct: 1   MEDFESYIILFFIWLVSTLTIRLIFAKNKHNSHLPPSPFALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120
           LS RHGPL+HLRLGSVPC+VVSS E AKQVLKT ESSFSNRPHL+AVDYLTYGSADFSFA
Sbjct: 61  LSNRHGPLMHLRLGSVPCVVVSSPETAKQVLKTQESSFSNRPHLSAVDYLTYGSADFSFA 120

Query: 121 PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRA 180
           PYG YWKFMKKLCMSELLG RTLDSL P+R+DE RRFLRSLQ+KA +GK+VDVG ELMR 
Sbjct: 121 PYGPYWKFMKKLCMSELLGGRTLDSLRPMREDEIRRFLRSLQSKAADGKEVDVGGELMRL 180

Query: 181 SNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLR 240
           SNNVISRMTLGKR AEEEEEAEGIR LVKD+A LTGTFNV+DYIWFCK LDLQ LGKRL+
Sbjct: 181 SNNVISRMTLGKRCAEEEEEAEGIRKLVKDIAVLTGTFNVSDYIWFCKNLDLQRLGKRLK 240

Query: 241 EVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK 300
           EVRERFDEMMER+I EHE++          D KV DLL ILLLKF DENSEVKLTRENIK
Sbjct: 241 EVRERFDEMMERMIKEHEDNC--------SDGKVKDLLDILLLKFGDENSEVKLTRENIK 298

Query: 301 AFILTH 307
           AF+L +
Sbjct: 301 AFVLVN 298

[Close]

BLAST of Carg12736 vs. TrEMBL
Match: tr|A0A1S3CCS8|A0A1S3CCS8_CUCME (cytochrome P450 93A2-like OS=Cucumis melo OX=3656 GN=LOC103499558 PE=3 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 9.9e-129
Identity = 237/304 (77.96%), Postives = 263/304 (86.51%), Query Frame = 0


Query: 1   MENFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60
           M +F SY ILF IWL+ST+TIR +FAK K N  LPP+P ALPIIGHLHLLGPLPHKAFHK
Sbjct: 1   MADFGSYIILFFIWLVSTLTIRLLFAKNKLNSHLPPSPFALPIIGHLHLLGPLPHKAFHK 60

Query: 61  LSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120
           LSTR+GPL+HLRLGSVPC+VVSS E AKQVLKTHESSFSNRPHL+AVDYLTYGSADFSFA
Sbjct: 61  LSTRYGPLMHLRLGSVPCVVVSSPETAKQVLKTHESSFSNRPHLSAVDYLTYGSADFSFA 120

Query: 121 PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRA 180
           PYG +WKFMKKLCMSELLG RTLDSL P R+DE RRF++ LQ+KA +GK+VDVG ELMR 
Sbjct: 121 PYGPFWKFMKKLCMSELLGGRTLDSLRPTREDEIRRFIQLLQSKAADGKEVDVGGELMRL 180

Query: 181 SNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLR 240
           SNNVISRMTLGKR AEEEEEAEGIR LVKD+A L+GTFNV+DYIWFCK LDLQ LGKRL+
Sbjct: 181 SNNVISRMTLGKRCAEEEEEAEGIRKLVKDIAVLSGTFNVSDYIWFCKNLDLQRLGKRLK 240

Query: 241 EVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK 300
           EVRERFDE+MER+I +HEE+          D KV DLL ILLLKF DEN+EVKLTRENIK
Sbjct: 241 EVRERFDELMERMIKDHEENC--------SDGKVKDLLDILLLKFGDENTEVKLTRENIK 296

Query: 301 AFIL 305
           AF+L
Sbjct: 301 AFVL 296

[Close]

BLAST of Carg12736 vs. TrEMBL
Match: tr|A0A2P6RDR4|A0A2P6RDR4_ROSCH (Putative 3,9-dihydroxypterocarpan 6A-monooxygenase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr3g0480431 PE=3 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 4.3e-108
Identity = 196/301 (65.12%), Postives = 240/301 (79.73%), Query Frame = 0


Query: 4   FQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHKLST 63
           FQ Y ILFL+WL+STI +R I AK + N RLPP+P+ALPIIGHLHLL P+PH+A HKLS 
Sbjct: 5   FQGYIILFLVWLVSTILVRTILAKFRTNSRLPPSPMALPIIGHLHLLAPIPHQALHKLSN 64

Query: 64  RHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFAPYG 123
           R GPL+H+ LGSVPC+VVSS EMAK+ LKTHE+SFSNRPH+AAVDYLTYGSADFSFAPYG
Sbjct: 65  RLGPLVHIFLGSVPCVVVSSPEMAKEFLKTHEASFSNRPHMAAVDYLTYGSADFSFAPYG 124

Query: 124 RYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRASNN 183
            YWKFMKKLCMSELLG RTLD L P+R++E   F+  +  KA   + VD+G ELM  +NN
Sbjct: 125 PYWKFMKKLCMSELLGGRTLDQLLPVRREELASFIDLMLNKAKSNEAVDLGAELMNVTNN 184

Query: 184 VISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLREVR 243
           +ISRM +G+  + +E+EA+ +R LVK VAELTG FN++D+IWFCK LDLQG GKRL+EVR
Sbjct: 185 IISRMLMGQSCSADEKEADEVRKLVKAVAELTGKFNLSDFIWFCKNLDLQGFGKRLKEVR 244

Query: 244 ERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIKAFI 303
           ++FD MM+R+I EH+E+ K  +  G  D+ V DLL IL     DE SE++LTRENIKAFI
Sbjct: 245 DQFDTMMQRIIKEHQEARKKRKEVGESDDDVKDLLDILFDISEDETSEIRLTRENIKAFI 304

Query: 304 L 305
           L
Sbjct: 305 L 305

[Close]

BLAST of Carg12736 vs. TrEMBL
Match: tr|A0A2I4G6D5|A0A2I4G6D5_9ROSI (cytochrome P450 93A3-like OS=Juglans regia OX=51240 GN=LOC109005129 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 9.0e-106
Identity = 196/304 (64.47%), Postives = 242/304 (79.61%), Query Frame = 0


Query: 1   MENFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60
           M + QSY +LFLIWL+STI +R IF + K   RLPP+P+ALPIIGHLHLL PLPH+A HK
Sbjct: 1   MADLQSYLVLFLIWLVSTILVRSIFTRFKTEARLPPSPMALPIIGHLHLLAPLPHQALHK 60

Query: 61  LSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120
           LS R+GPLIHL LGSVPC+V SS EMAK+ LKTHE+SF NRP  AAVDYLTYGS+DFSFA
Sbjct: 61  LSNRYGPLIHLFLGSVPCVVASSPEMAKEFLKTHETSFCNRPQTAAVDYLTYGSSDFSFA 120

Query: 121 PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRA 180
           PYG YWKFMKKLCMSELLG RTLD L P+R++E  +F++ L  KA  G+ VDVG +L+R 
Sbjct: 121 PYGPYWKFMKKLCMSELLGGRTLDQLLPVRREEITKFVQFLLNKAEAGEAVDVGGQLLRV 180

Query: 181 SNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLR 240
           +NNVISRM++ +R +E E EA+ +  LVK +AELTG FN++D+IWFCK LDLQGL KRL+
Sbjct: 181 TNNVISRMSMSERCSENEHEADDVAKLVKAIAELTGKFNLSDFIWFCKNLDLQGLRKRLK 240

Query: 241 EVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK 300
           EV +RFD +MER++ EHEE+ K  E E +G   V DLL ILL    D+++E++L+RENIK
Sbjct: 241 EVHDRFDSLMERILQEHEEARK-KEKERDGAYAVKDLLDILLDISEDQSAEMRLSRENIK 300

Query: 301 AFIL 305
           AFIL
Sbjct: 301 AFIL 303

[Close]

BLAST of Carg12736 vs. TrEMBL
Match: tr|B9II24|B9II24_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_016G049800v3 PE=3 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 5.8e-105
Identity = 194/304 (63.82%), Postives = 241/304 (79.28%), Query Frame = 0


Query: 1   MENFQSYTILFLIWLLSTITIRFIFAKTKPNPRLPPTPLALPIIGHLHLLGPLPHKAFHK 60
           M + Q Y ILFL+WLLSTI +R I  KT+  PRLPP+PLALPIIGHLHLL P+PH+A HK
Sbjct: 1   MADIQGYIILFLLWLLSTILVRAILNKTRAKPRLPPSPLALPIIGHLHLLAPIPHQALHK 60

Query: 61  LSTRHGPLIHLRLGSVPCIVVSSAEMAKQVLKTHESSFSNRPHLAAVDYLTYGSADFSFA 120
           LSTR+GPLIHL LGSVPC+V S+ E AK+ LKTHE+SF +RP   AVD+LTYGSADFSFA
Sbjct: 61  LSTRYGPLIHLFLGSVPCVVASTPETAKEFLKTHENSFCDRPKSTAVDFLTYGSADFSFA 120

Query: 121 PYGRYWKFMKKLCMSELLGSRTLDSLAPIRQDETRRFLRSLQTKAVEGKDVDVGRELMRA 180
           PYG YWKFMKK+CM+ELLG R LD L P++ +E R+FL+ L  KA   + +DVG +L+R 
Sbjct: 121 PYGPYWKFMKKICMTELLGGRMLDQLLPVKHEEIRQFLQFLLKKANARESIDVGSQLIRL 180

Query: 181 SNNVISRMTLGKRSAEEEEEAEGIRMLVKDVAELTGTFNVADYIWFCKGLDLQGLGKRLR 240
           +NNVISRM + +R ++ ++EA+ +R LV +VA+LTG FN++D+IWFCK LDLQG GKRL+
Sbjct: 181 TNNVISRMAMSQRCSDNDDEADEVRNLVHEVADLTGKFNLSDFIWFCKNLDLQGFGKRLK 240

Query: 241 EVRERFDEMMERLIMEHEESLKMMEMEGNGDEKVMDLLHILLLKFRDENSEVKLTRENIK 300
           EVR+RFD M ER+IMEHEE+ K  +  G GD  V DLL ILL    D++SE+KLTRENIK
Sbjct: 241 EVRKRFDTMTERIIMEHEEARKKKKETGEGD-PVKDLLDILLDISEDDSSEMKLTRENIK 300

Query: 301 AFIL 305
           AFIL
Sbjct: 301 AFIL 303

The following BLAST results are available for this feature:

BLAST of Carg12736 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023518782.1	6.9e-166	99.01	cytochrome P450 93A2 [Cucurbita pepo subsp. pepo]	[more]
XP_022925132.1	9.0e-166	98.68	cytochrome P450 93A2 [Cucurbita moschata]	[more]
XP_022966275.1	1.2e-162	97.04	cytochrome P450 93A2 [Cucurbita maxima]	[more]
XP_004150165.1	1.7e-132	80.59	PREDICTED: cytochrome P450 93A2-like [Cucumis sativus]	[more]
KGN53825.1	1.7e-132	80.07	hypothetical protein Csa_4G152790 [Cucumis sativus]	[more]

BLAST of Carg12736 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G06900.1	9.8e-74	46.41	cytochrome P450, family 93, subfamily D, polypeptide 1	[more]
AT2G42250.1	1.6e-52	40.07	cytochrome P450, family 712, subfamily A, polypeptide 1	[more]
AT1G50560.1	1.8e-51	38.46	cytochrome P450, family 705, subfamily A, polypeptide 25	[more]
AT2G05180.1	2.6e-50	39.74	cytochrome P450, family 705, subfamily A, polypeptide 6	[more]
AT1G50520.1	5.8e-50	38.26	cytochrome P450, family 705, subfamily A, polypeptide 27	[more]

BLAST of Carg12736 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q42798\|C93A1_SOYBN	3.9e-80	50.66	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1...	[more]
sp\|O81973\|C93A3_SOYBN	3.9e-80	52.48	Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1	[more]
sp\|Q42799\|C93A2_SOYBN	6.7e-80	52.33	Cytochrome P450 93A2 OS=Glycine max OX=3847 GN=CYP93A2 PE=2 SV=1	[more]
sp\|Q5VRI5\|C93G2_ORYSJ	3.2e-66	48.21	Cytochrome P450 93G2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP93G2 PE=2 SV...	[more]
sp\|Q9XHC6\|C93E1_SOYBN	7.2e-66	44.77	Beta-amyrin 24-hydroxylase OS=Glycine max OX=3847 GN=CYP93E1 PE=1 SV=1	[more]

BLAST of Carg12736 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KW21\|A0A0A0KW21_CUCSA	1.1e-132	80.07	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G152790 PE=4 SV=1	[more]
tr\|A0A1S3CCS8\|A0A1S3CCS8_CUCME	9.9e-129	77.96	cytochrome P450 93A2-like OS=Cucumis melo OX=3656 GN=LOC103499558 PE=3 SV=1	[more]
tr\|A0A2P6RDR4\|A0A2P6RDR4_ROSCH	4.3e-108	65.12	Putative 3,9-dihydroxypterocarpan 6A-monooxygenase OS=Rosa chinensis OX=74649 GN...	[more]
tr\|A0A2I4G6D5\|A0A2I4G6D5_9ROSI	9.0e-106	64.47	cytochrome P450 93A3-like OS=Juglans regia OX=51240 GN=LOC109005129 PE=3 SV=1	[more]
tr\|B9II24\|B9II24_POPTR	5.8e-105	63.82	Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_016G049800v3 PE=...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0005506	iron ion binding
GO:0020037	heme binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450
IPR036396	Cyt_P450_sf
IPR002401	Cyt_P450_E_grp-I

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12736-RA	Carg12736-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 232..252
None	No IPR available	PANTHER	PTHR24298:SF129	SUBFAMILY NOT NAMED	coord: 4..304
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 4..304
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 86..107 score: 26.31 coord: 62..81 score: 31.51 coord: 180..198 score: 27.32
IPR036396	Cytochrome P450 superfamily	GENE3D	G3DSA:1.10.630.10		coord: 26..306 e-value: 1.7E-59 score: 203.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	SSF48264	Cytochrome P450	coord: 30..305
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 35..305 e-value: 2.2E-35 score: 122.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg12736	Cla000744	Watermelon (97103) v1	carwmB0277
Carg12736	CmaCh07G013130	Cucurbita maxima (Rimu)	carcmaB0398
Carg12736	CmoCh07G013570	Cucurbita moschata (Rifu)	carcmoB0395
Carg12736	Cp4.1LG19g00680	Cucurbita pepo (Zucchini)	carcpeB0400
Carg12736	Bhi03G002129	Wax gourd	carwgoB0373

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg12736	Silver-seed gourd	carcarB230
Carg12736	Cucumber (Gy14) v2	carcgybB0299
Carg12736	Cucumber (Gy14) v1	carcgyB0332
Carg12736	Cucurbita maxima (Rimu)	carcmaB0397
Carg12736	Cucurbita moschata (Rifu)	carcmoB0394
Carg12736	Cucurbita pepo (Zucchini)	carcpeB0398
Carg12736	Wild cucumber (PI 183967)	carcpiB0318
Carg12736	Cucumber (Chinese Long) v2	carcuB0313
Carg12736	Cucumber (Chinese Long) v3	carcucB0317
Carg12736	Bottle gourd (USVL1VR-Ls)	carlsiB259
Carg12736	Bottle gourd (USVL1VR-Ls)	carlsiB260
Carg12736	Melon (DHL92) v3.5.1	carmeB0288
Carg12736	Melon (DHL92) v3.6.1	carmedB0299
Carg12736	Watermelon (Charleston Gray)	carwcgB0276
Carg12736	Watermelon (97103) v1	carwmB0274
Carg12736	Watermelon (97103) v1	carwmB0278
Carg12736	Watermelon (97103) v2	carwmbB0292
Carg12736	Wax gourd	carwgoB0371