Carg12409 (gene) Silver-seed gourd

NameCarg12409
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionEnolase
LocationCucurbita_argyrosperma_scaffold_074 : 798262 .. 804387 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGATATATAGGAGTTGGGGCAGGCTTCCAGTGGAACGGCACCGTGCGATGTTGCGAAGATCTCCATAGTTTAACCCAAGGTCTCGTTACTATACGTTCGGCGGCAACACAAGAACAGAGCTCGGCTAGCAAGCTTCATAACTTCACTCCGTCTTGCAGGATCCAATCTCTGGATTTTTCTCCACCAATTTTCCATGGAGAAGAAGCAGCTGAAAGAAATGTCCGTTCAAGAGTATCTGGACAAGTACATGCTCTCCCGGAAAATCGAGGAAGCGGTCAATGCTGCTGTTAGGGCGAAAACTCCCGATCCTGTTCTATTCATTGTAATCTCCCCCTCTATCTCTCTGTTCTTGGTTCTTAGAAAATGCTGGTGGAAATTTAATTGGACTGTTTTTTTGTTTTTTTCTGAACAATCAATATACGAAACTAGATGGAACGAATGCTTGTCGATGTAGTTGACGATCATGAACGCAAAATTTCCGTTGATTTTTATTTCTCATGGATCAATTGTACTTAATTGATTCGTTTCGCAGTTCATTCTTTATCTCTATTTACTTTATTATTATTATTTTTTTTTTTTGTTTCGAACAGTCAAATCATATGAAGAAAGCGGTTCCTTCATTGATTACAAAAATTAAAGCTCGGCAGATCCTTGACAGTAGAGGAATTCCGACTGTTGAAGTCGATTTGTACACCAACAAAGGAGTGTTCCACGCTTCCGTTCCCAGCGGCGATCCGGCTGGCATGTAAGGTTCCCAGAGATGAGTAGACTTGAATTGGGAAGGATATAATAATCTTCATTGGCAAAACTTTTTGTTAGAGAGTGATCTGCGAAACGTTTGGCTGTCATTTTCAATTTCAACGCATGGTTTCCTCATGCCTGTTGAGTTCCTATACATTTTTTTTCCAATTGGATTTTAAGACGAGGGTTGCTTTTCCTAGGTATGAGGCTGTGGACTTGCGTGATGGAGATAAGGGAACATACCTTGGAAATAGTGTTACTAAAGCAGTAAAAAATATCAACGACAAGATATCTGAAGCATTGATTGGTAGGGATCCTACTCAACAATATGAAATTGACCAGGCCATGAAAGATTTGGATACGACAGAAAAAAAGGTGCCAATACATCTCCTTGTCCTGAATTCACAAATTTTATGGAAGTGTAACTTGTATGCCATCTTCTGTATTATTACTGTGCTCTACTATATCTGGTACTCGCTGGTACAATTGCAATGTTAATGGATGGGGAAGATTGAATTTACTTAATATCCTATACGACACTGTCTATTAGGAGGCAGGAGGAATTATTTATTATGCTGAATTATTTATCACGAGCATATGTATCATAGTTTACTTTGTTTTGTCATAAATTTAAATTTCAGCCTGCCTGGAAGATTTTCTACACTGATATCTCAGATTCTCAACTCAATATGAAAGAAGAAAAACTTTGCATATATATTGATTTAGAGTCACACGGATCAATGCTACCCTTTTTGTTTGAAATAGGACATAATAGTTTTTCATTATTTGTTATTCCTATCTTTGCTATTTTGGATATGTCATTAGGACTTTATGATAATGTGTCTGTCTGTCTATATAATAGATTGTTTATGATTAGAAAATATTTACATTGGATAGTTACTTCTTTGGAAAGTAGTTTATTTTCAAAGGCATCAATAGTGATACCTTTTAAGTTAGTATGTCTCTTAGTCAATGCACACTGCAGGGTGAACTTGGAGCAAACGCTATACTTGCCGTGTCAATTGCAGTATGCAGAGCTGGTGCTGCTGAAAAGGAGGCATGCTTTTTATTCACCTCTGTTACATGGAATTGTTTTTTCTGTTTATGGGGCATTTCTACAATTCTGCAAAGACATTTAAACCAAGTATTTTCAGGTTCCACTCTATAAGCATATTGCAGATCTTGCTGGCAAAACAAACTTGATACTTCCTGTCCCAGCCTTCACTGTAATTAGTGGAGGAAAACATGCTGGCAATAATCTGGCCATTCAGGTATGCTGTGGTAAGGATTCAAAGCAGTTGTGATCTTTTTTTTTTTCTTCTTCTTCTTCTTCTTCTTTTTTTTTTTTGTTAGATGACTAGAGTGTTTTTTTTACCGCATGCAGCAAATAATCTATCTAAGAAACCATTGACTCTGAATGTAATCTTTCTGTCTAGTAAATCAATCTGTACACAATGATATAGATGGAAGCCATTGTGGCTTATGACTATGATAGTTTCTATTGATTTAGAAGGATTCCAAATGTTGTATGCTGTGTCTATTATTTTAGATTGACTGGTATTGTAATCAGGAAATCATGATTCTTCCAATTGGAGCTAGCAAGTTTGAAGAGGCATTAAAAATGGGCTCTGAAACTTTCCATCACTTAAAGGTGCGATTTCATTCACCACAACCGTTGAATGGAGTTTTAATTCATGTCGTCAATGCATTCAATTATGCTTATTTATAACTTTAGGTGTATTACATGGTGAAAGTACCAGTTCAAATATCTTGTAGAGAAAAATCTTCTTCTTCTTCTTCTTATTTCTGTGCACGATCATCCATGATACTCATATGTATATCTATATTATCAGATAGAATGTATTACTATTTTAATTTCACAATTTATTGAGGATGTACGTGTCTTTTTTTTGTCTTGTGTGTGTGTGTGTGTGTGTATTTTCTAAGTATTACACCTCATTGAAGGATCTAAGTATCAAAAGTATATCTTAATCTTCTATTTTAGCTTTTAAATTCTTCTTCTCATAGCATATCACATGCATAGCACTAAGTTTAGTGATTCATAGTCGTCCATATACTTGTCCAAAACCTTGAGGCACTCTCTGCCTCTTGGCATGCTCTATTATTGTTGTCCTGTGTTATGTTCCTTAACAATTATTATAGCTTACCAAGTGTGTTGAAAATGCCAAAATCATAGACTAACATTTTGCAAAGTTGTGCATGGTTTAATCACTTATCATTCTACTTATGAATAATGTCATTCTATTTTCCTGTGGGTCTTTGCTTTTTCGTACTTAACTATCAAAGTTTGTTCTATTTGGAAGCAACATTGTTAATTATTCCATATATTACTAAAAATGTAATTTCCTTGGAAAAAATGGCAGGCTGTAATTTCTGAAAAGCATGGTGCCCATGGATGTAATTTTGGTGAAGATGGTGGATTTGCTCCAAATATATCCAGGCAAGATACAAGTTCCAAAATTTCTTGGTTTGTTCATATGGATCTGTGGAGCTTTTTTTTTTTTTTCCTAGCCATCAAGAAGAACATTCTAGCATGAGAAGGATAGATTACACGTTTGGCGAACCCTGCATACTACATTTACTTTTACAGTTATCGGCTGCTTGTCTGAGTCTTTGACACAGCGCTTGATCCAGAAACATGTTTTTAATGAGTTGTATTCTATCAACAGTTTTACAGAAGCTCTGGATCTTGTACAAGAGGCTATTGGCAGAACTGGGTATAACGAGAGAATCAAGATAGCAATAGATATTGCTGCTACTAATTTCTGCATGGGTGAGAGAAACAAGTTTCCTAAATAATAGCCTATTTGAACAATAGGATATGAAAAACTGGAAGAAGGGATCAAAAAATGCTTTATTGGATTCGTACTACTTGCTGATAATCCACGTTCATCACCCTTTCTCCAGTTATTTTCCTTACAGATGGTCTCCATAAGGAAAACCCGTTAATCTACTCACATGAAATAAATGTTTCCTTTCCTGCAGCAAAACTTACCCTAAAATTACATTTTTTCTCATTCACTGTAGGTACCAAATATGATCTTGACTTCAAAGCTCCAAACAAGTTAGGTCAAAATTTCAAGTCAGGGAAGGATATGATAGATATGTACAAAGAACTATGTGATGGTATATATGTCTCTTGTTCTGCTCAATCATTAGGCGTTTTTGTTGCTTGTTTTCAACAAAGGGTTAACATTTCTGTTCTCATGTTTTTAGACTATCCAATTGTATCAATTGAGGATCCATTTGACCGGGAGGACTGGGATCACACCAAACATTTTTCTGGCCTTGGAATTTGTCAGGTTTCTCCTCATACTTCTCCTGAGTAACGTCCGGAATAATTATATACAAAGTGTTTACATTGTTAGAATATTTATTTTAACTAAAAAGGACTGGAGATTGTGGTTAAAGATCCGTGGTCTAGGGTTGGACCTAATAGTTAAAAGATATTTGTATCATCTGTCACAAGAGGTTGTGAGTTAAAATTCCCCGACACCACTTGTAAATTTAGAATGTCAATATGCCCCTAGTTTCTTGTCTGACTATGTATGTTACTATTGGGTCAGATTATCGTCCTTGATTTCAAGCATGATGATCTTGTGGAATGTCAATTTGATTGCCCTTTTTAACATCAAAGGAATAACTAGCAAATTTGTAGGATACAGTTAACCAAACTGTCAAAGAAGAAGATAACTTGATTGCTTTGCATCTTATAGCAATTGAGGAGGCTTCATGATGTTTTTCTTACTGGAGAAAAAGACACGATCTTTTATGATTTATACAAGTTAAATGTTTTCTTGACCTTCTGGATACGGAAGTGTTTTTTTCCCCTTTTTAACCGCTCAATGCTTCTTTAATGATTTTAGTTTTCTGTAGGTAGTGGGAGGTGATTTGTTAATGTCAAACAAAAAGCGAATTGAGAGGGCAATTGATGAGACCACTTGTAATGCCCTCCTCCTCAAGGTACAAATCACTATTTTATATTTTTATTATATAAATGTAACATTTCTTTGTCTTCTTTTGGACATACTGTGGAATATTCTCCTGTTGGTACTGTTCATAACACCATTAATCCTTCGAGGCTATGGAGAATTGGAAATAAATACCAACTGACCTTTTGCAAGTGTACTTTGGTGCAAAATGCAACGTTGTACCGTGAAGTCTGCTTTAATGGAAATTATGCTGAAAAAATTGTTTACTTTCTATTCTGTAGTGTAAGTCCCCCAATTTGGCTTTCAAATTTTGATAATTTCATGGGACAAAAACGTAGGTAAATCAGATTGGCACCGTCACAGAAGCCATTGAAGTCGTGAACCTTGCCAAGGATGCACAATGGGCAGTTGTGGCGTCTCACAGATGCGGTGAAACAGACGATACATTCCTAGCTGATTTATCTGTTGGACTTTCCACAGGTCAGATCAAAGCGGGTGCTCCGTGCAGAGGAGAACGTCTTGCCAAATATAATCAGGTCTAATTTCTTGAACATTTGCCTAGCCTAATTTTTGTCGTTTTTATGTCACATACAAGCTAGTGAAAGAGGTGGTGTTAACTAGAAACAACTTAGTTATTCTTTTGGGGACATCCGATCAAATTGGAACAATACAAAGAAAATTAGCATGACCCCTTTGCAAGGATGACAGTTTTTTTCGTATTACATTGCCTCACCTGATTATTAAAAGCAAGTAGACTGTCCGATCCCATTTTACAAAATAACAAGATCACAATTTCGTTTTAGTCAACGTAGAAATGCAACTAATAGTCCCGAGGTTAATGCAGTTGCTCCGTATCGAAGAAGAGCTCGGAGATCAAGCAGTTTACGCCGGTGAAGATTGGAGGACGATGTACTGATTTTCGTTTGTTTTATACCGCCATTTTTCTCAACTCGGTTCGATTTCCTTCCCATGCCTTGACAACATGGTACAACTCCAACTAGTGTTCATTTATTTTCTCTACTTATTTTCATCAGTATAGAAACATTAGAACAGTAGGGGTGGCCTAAAATATTGTGTGCTTGTTTTCTGTTTTTCTTTCCAACTTTAACCAGCAAGTTTTGTGATCCTGGGCACATATTACTGGATTAGAAAAGTCTATCTATCATCGTTTCTAGGCACATATTTACTAATACTTTCTGAGATTCTTTTTCTTTCCCTTTGGAACTGAATTCATTTTTTAGCTCAATAATCTATTGAGCTGTTTAGTTGCTGTTGTTTCATCATATATTTGTTTGGATTGATATTTAAAAGAAAAAAGAAAGTTGAAATACAAATTGGGTACTTATGTCTAGGCTAATTTCCAATCTAGTCCTTATTTTTTCGATTTTTTGGATGGTAATTGATATTTTCTCGTACAAATATAACATTTATT

mRNA sequence

AGATATATAGGAGTTGGGGCAGGCTTCCAGTGGAACGGCACCGTGCGATGTTGCGAAGATCTCCATAGTTTAACCCAAGGTCTCGTTACTATACGTTCGGCGGCAACACAAGAACAGAGCTCGGCTAGCAAGCTTCATAACTTCACTCCGTCTTGCAGGATCCAATCTCTGGATTTTTCTCCACCAATTTTCCATGGAGAAGAAGCAGCTGAAAGAAATGTCCGTTCAAGAGTATCTGGACAAGTACATGCTCTCCCGGAAAATCGAGGAAGCGGTCAATGCTGCTGTTAGGGCGAAAACTCCCGATCCTGTTCTATTCATTTCAAATCATATGAAGAAAGCGGTTCCTTCATTGATTACAAAAATTAAAGCTCGGCAGATCCTTGACAGTAGAGGAATTCCGACTGTTGAAGTCGATTTGTACACCAACAAAGGAGTGTTCCACGCTTCCGTTCCCAGCGGCGATCCGGCTGGCATGTATGAGGCTGTGGACTTGCGTGATGGAGATAAGGGAACATACCTTGGAAATAGTGTTACTAAAGCAGTAAAAAATATCAACGACAAGATATCTGAAGCATTGATTGGTAGGGATCCTACTCAACAATATGAAATTGACCAGGCCATGAAAGATTTGGATACGACAGAAAAAAAGGGTGAACTTGGAGCAAACGCTATACTTGCCGTGTCAATTGCAGTATGCAGAGCTGGTGCTGCTGAAAAGGAGGTTCCACTCTATAAGCATATTGCAGATCTTGCTGGCAAAACAAACTTGATACTTCCTGTCCCAGCCTTCACTGTAATTAGTGGAGGAAAACATGCTGGCAATAATCTGGCCATTCAGGAAATCATGATTCTTCCAATTGGAGCTAGCAAGTTTGAAGAGGCATTAAAAATGGGCTCTGAAACTTTCCATCACTTAAAGGCTGTAATTTCTGAAAAGCATGGTGCCCATGGATGTAATTTTGGTGAAGATGGTGGATTTGCTCCAAATATATCCAGTTTTACAGAAGCTCTGGATCTTGTACAAGAGGCTATTGGCAGAACTGGGTATAACGAGAGAATCAAGATAGCAATAGATATTGCTGCTACTAATTTCTGCATGGGTACCAAATATGATCTTGACTTCAAAGCTCCAAACAAGTTAGGTCAAAATTTCAAGTCAGGGAAGGATATGATAGATATGTACAAAGAACTATGTGATGACTATCCAATTGTATCAATTGAGGATCCATTTGACCGGGAGGACTGGGATCACACCAAACATTTTTCTGGCCTTGGAATTTGTCAGGTAGTGGGAGGTGATTTGTTAATGTCAAACAAAAAGCGAATTGAGAGGGCAATTGATGAGACCACTTGTAATGCCCTCCTCCTCAAGGTAAATCAGATTGGCACCGTCACAGAAGCCATTGAAGTCGTGAACCTTGCCAAGGATGCACAATGGGCAGTTGTGGCGTCTCACAGATGCGGTGAAACAGACGATACATTCCTAGCTGATTTATCTGTTGGACTTTCCACAGGTCAGATCAAAGCGGGTGCTCCGTGCAGAGGAGAACGTCTTGCCAAATATAATCAGTTGCTCCGTATCGAAGAAGAGCTCGGAGATCAAGCAGTTTACGCCGGTGAAGATTGGAGGACGATGTACTGATTTTCGTTTGTTTTATACCGCCATTTTTCTCAACTCGGTTCGATTTCCTTCCCATGCCTTGACAACATGGTACAACTCCAACTAGTGTTCATTTATTTTCTCTACTTATTTTCATCAGTATAGAAACATTAGAACAGTAGGGGTGGCCTAAAATATTGTGTGCTTGTTTTCTGTTTTTCTTTCCAACTTTAACCAGCAAGTTTTGTGATCCTGGGCACATATTACTGGATTAGAAAAGTCTATCTATCATCGTTTCTAGGCACATATTTACTAATACTTTCTGAGATTCTTTTTCTTTCCCTTTGGAACTGAATTCATTTTTTAGCTCAATAATCTATTGAGCTGTTTAGTTGCTGTTGTTTCATCATATATTTGTTTGGATTGATATTTAAAAGAAAAAAGAAAGTTGAAATACAAATTGGGTACTTATGTCTAGGCTAATTTCCAATCTAGTCCTTATTTTTTCGATTTTTTGGATGGTAATTGATATTTTCTCGTACAAATATAACATTTATT

Coding sequence (CDS)

ATGGAGAAGAAGCAGCTGAAAGAAATGTCCGTTCAAGAGTATCTGGACAAGTACATGCTCTCCCGGAAAATCGAGGAAGCGGTCAATGCTGCTGTTAGGGCGAAAACTCCCGATCCTGTTCTATTCATTTCAAATCATATGAAGAAAGCGGTTCCTTCATTGATTACAAAAATTAAAGCTCGGCAGATCCTTGACAGTAGAGGAATTCCGACTGTTGAAGTCGATTTGTACACCAACAAAGGAGTGTTCCACGCTTCCGTTCCCAGCGGCGATCCGGCTGGCATGTATGAGGCTGTGGACTTGCGTGATGGAGATAAGGGAACATACCTTGGAAATAGTGTTACTAAAGCAGTAAAAAATATCAACGACAAGATATCTGAAGCATTGATTGGTAGGGATCCTACTCAACAATATGAAATTGACCAGGCCATGAAAGATTTGGATACGACAGAAAAAAAGGGTGAACTTGGAGCAAACGCTATACTTGCCGTGTCAATTGCAGTATGCAGAGCTGGTGCTGCTGAAAAGGAGGTTCCACTCTATAAGCATATTGCAGATCTTGCTGGCAAAACAAACTTGATACTTCCTGTCCCAGCCTTCACTGTAATTAGTGGAGGAAAACATGCTGGCAATAATCTGGCCATTCAGGAAATCATGATTCTTCCAATTGGAGCTAGCAAGTTTGAAGAGGCATTAAAAATGGGCTCTGAAACTTTCCATCACTTAAAGGCTGTAATTTCTGAAAAGCATGGTGCCCATGGATGTAATTTTGGTGAAGATGGTGGATTTGCTCCAAATATATCCAGTTTTACAGAAGCTCTGGATCTTGTACAAGAGGCTATTGGCAGAACTGGGTATAACGAGAGAATCAAGATAGCAATAGATATTGCTGCTACTAATTTCTGCATGGGTACCAAATATGATCTTGACTTCAAAGCTCCAAACAAGTTAGGTCAAAATTTCAAGTCAGGGAAGGATATGATAGATATGTACAAAGAACTATGTGATGACTATCCAATTGTATCAATTGAGGATCCATTTGACCGGGAGGACTGGGATCACACCAAACATTTTTCTGGCCTTGGAATTTGTCAGGTAGTGGGAGGTGATTTGTTAATGTCAAACAAAAAGCGAATTGAGAGGGCAATTGATGAGACCACTTGTAATGCCCTCCTCCTCAAGGTAAATCAGATTGGCACCGTCACAGAAGCCATTGAAGTCGTGAACCTTGCCAAGGATGCACAATGGGCAGTTGTGGCGTCTCACAGATGCGGTGAAACAGACGATACATTCCTAGCTGATTTATCTGTTGGACTTTCCACAGGTCAGATCAAAGCGGGTGCTCCGTGCAGAGGAGAACGTCTTGCCAAATATAATCAGTTGCTCCGTATCGAAGAAGAGCTCGGAGATCAAGCAGTTTACGCCGGTGAAGATTGGAGGACGATGTACTGA

Protein sequence

MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKNINDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFHHLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATNFCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSGLGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVASHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWRTMY
BLAST of Carg12409 vs. NCBI nr
Match: XP_022949451.1 (cytosolic enolase 3 [Cucurbita moschata] >XP_023525808.1 cytosolic enolase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 964.5 bits (2492), Expect = 1.3e-277
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120

Query: 121 INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300
           HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN
Sbjct: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300

Query: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360
           FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG
Sbjct: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420
           LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420

Query: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480

Query: 481 TMY 484
           TMY
Sbjct: 481 TMY 483

BLAST of Carg12409 vs. NCBI nr
Match: XP_022998824.1 (cytosolic enolase 3 [Cucurbita maxima])

HSP 1 Score: 954.9 bits (2467), Expect = 1.0e-274
Identity = 478/483 (98.96%), Postives = 482/483 (99.79%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60
           MEKKQLK+MSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA
Sbjct: 1   MEKKQLKQMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDL+TNKGVF+ASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLHTNKGVFNASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120

Query: 121 INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           INDKISEALIGRDPTQQYEIDQAMK+LDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INDKISEALIGRDPTQQYEIDQAMKNLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300
           HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN
Sbjct: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300

Query: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360
           FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG
Sbjct: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420
           LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420

Query: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480

Query: 481 TMY 484
            MY
Sbjct: 481 AMY 483

BLAST of Carg12409 vs. NCBI nr
Match: XP_004145264.1 (PREDICTED: cytosolic enolase 3 [Cucumis sativus] >KGN65781.1 hypothetical protein Csa_1G528460 [Cucumis sativus])

HSP 1 Score: 922.2 bits (2382), Expect = 7.5e-265
Identity = 461/480 (96.04%), Postives = 470/480 (97.92%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKA+PS+ITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAV+LRDGDKGTYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 121 INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           IN+KISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLAGKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300
           HLKAVI+EKHGAHGCNFGEDGGFAPNISSF EALDLV+EAI R GYNERIKIAID+AATN
Sbjct: 241 HLKAVITEKHGAHGCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360
           FCMGTKYDLDFKAPNK  QNFKSGKDMIDMYKELC DYPIVSIEDPFDREDWDHTKHFS 
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420
           LGICQVVGGDLLMSNKKRIERAIDE TCNALLLKVNQIGTVTEAIEVV LAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLADLSVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQA+YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

BLAST of Carg12409 vs. NCBI nr
Match: XP_008457408.1 (PREDICTED: cytosolic enolase 3 [Cucumis melo])

HSP 1 Score: 919.5 bits (2375), Expect = 4.9e-264
Identity = 460/480 (95.83%), Postives = 469/480 (97.71%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKA+PS+ITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAV+LRDGDKGTYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 121 INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           IN+KISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLAGKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300
           HLKAVI+EKHGAH CNFGEDGGFAPNISSF EALDLV+EAI R GYNERIKIAID+AATN
Sbjct: 241 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360
           FCMGTKYDLDFKAPNK  QNFKSGKDMIDMYKELC DYPIVSIEDPFDREDWDHTKHFS 
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420
           LGICQVVGGDLLMSNKKRIERAIDE TCNALLLKVNQIGTVTEAIEVV LAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLADLSVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQA+YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

BLAST of Carg12409 vs. NCBI nr
Match: XP_022150946.1 (cytosolic enolase 3 [Momordica charantia])

HSP 1 Score: 907.9 bits (2345), Expect = 1.5e-260
Identity = 453/483 (93.79%), Postives = 466/483 (96.48%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKT DPVLFISNHMKKA+PS+ITK+KA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN 120

Query: 121 INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           IN+KISEALIGRDPTQQYEIDQAMKDLDTTEKK ELGANAILAVSIAVCRAGAAEK+VPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADL+GKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300
           HLKAVI+EKHGA GCNFG+DGG APNISSF E LDLVQEAIGRTGYNERIKIAID+AATN
Sbjct: 241 HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360
           FCMGTKYDLDFKAPNK  QNFKSGKDMIDMYKELC DYPIVSIEDPFDREDWDHTK+FS 
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420
           LG+CQVVGGDLLMSNK RIERAIDE+TCNALLLKVNQIGTVTEAI+VV LAK AQW VVA
Sbjct: 361 LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA 420

Query: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLAD SVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR
Sbjct: 421 SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480

Query: 481 TMY 484
           T Y
Sbjct: 481 TTY 483

BLAST of Carg12409 vs. TAIR10
Match: AT2G29560.1 (cytosolic enolase)

HSP 1 Score: 784.3 bits (2024), Expect = 4.4e-227
Identity = 382/472 (80.93%), Postives = 430/472 (91.10%), Query Frame = 0

Query: 9   MSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKARQILDSRG 68
           MSVQEYLDK+MLSRKIE+AVNAAVRAKT DPVLFI+NH+KKAV S+ITK+KARQILDSRG
Sbjct: 1   MSVQEYLDKHMLSRKIEDAVNAAVRAKTSDPVLFIANHLKKAVSSVITKVKARQILDSRG 60

Query: 69  IPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKNINDKISEA 128
           IPTVEVDL+TNKGVF ASVPSGD +G YEA++LRDGDKG YLGNSV KAVKNIN+KISEA
Sbjct: 61  IPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSVAKAVKNINEKISEA 120

Query: 129 LIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPLYKHIADLA 188
           LIG DP  Q +IDQAM DLD TEKK ELGANAILAVSIA C+AGAAEKEVPL KH++DL+
Sbjct: 121 LIGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAAEKEVPLCKHLSDLS 180

Query: 189 GKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFHHLKAVISE 248
           G+ N++LPVPAFTV+SGGKHA N  AIQEIMILPIGAS+FEEAL+ GSET+HHLKAVISE
Sbjct: 181 GRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGASRFEEALQWGSETYHHLKAVISE 240

Query: 249 KHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATNFCMGTKYD 308
           K+G  GCN GEDGG AP+ISS  E L+LV+EAI RTGYN++IKIAIDIAATNFC+GTKYD
Sbjct: 241 KNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTGYNDKIKIAIDIAATNFCLGTKYD 300

Query: 309 LDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSGLGICQVVG 368
           LD K+PNK GQNFKS +DMIDMYKE+C+DYPIVSIEDPFD+EDW+HTK+FS LGICQVVG
Sbjct: 301 LDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWEHTKYFSSLGICQVVG 360

Query: 369 GDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVASHRCGETD 428
            DLLMSN KR+ERAI E++CNALLLKVNQIGTVTEAIEVV +A+DAQW VV SHRCGET+
Sbjct: 361 DDLLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARDAQWGVVTSHRCGETE 420

Query: 429 DTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 481
           D+F++DLSVGL+TG IKAGAPCRGER  KYNQLLRIEEELGDQAVYAGEDW+
Sbjct: 421 DSFISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAVYAGEDWK 472

BLAST of Carg12409 vs. TAIR10
Match: AT2G36530.1 (Enolase)

HSP 1 Score: 491.9 bits (1265), Expect = 4.5e-139
Identity = 255/436 (58.49%), Postives = 324/436 (74.31%), Query Frame = 0

Query: 55  ITKIKARQILDSRGIPTVEVDLYTNKGV-FHASVPSGDPAGMYEAVDLRDGDKGTYLGNS 114
           IT +KARQI DSRG PTVEVD++T+ G+   A+VPSG   G+YEA++LRDG    YLG  
Sbjct: 4   ITVVKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGG-SDYLGKG 63

Query: 115 VTKAVKNINDKISEALIGRDPTQQYEIDQAM-KDLDTTEK-----KGELGANAILAVSIA 174
           V+KAV N+N+ I  ALIG+DPTQQ  ID  M  +LD T+      K +LGANAILAVS+A
Sbjct: 64  VSKAVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVSLA 123

Query: 175 VCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASK 234
           VC+AGA    +PLYKHIA+LAG   ++LPVPAF VI+GG HAGN LA+QE MILP+GA+ 
Sbjct: 124 VCKAGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAAS 183

Query: 235 FEEALKMGSETFHHLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYN 294
           F+EA+KMG E +HHLK+VI +K+G    N G++GGFAPNI    E L+L++ AI + GY 
Sbjct: 184 FKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGYT 243

Query: 295 ERIKIAIDIAATNFCMGTK-YDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDP 354
            ++ I +D+AA+ F    K YDL+FK  N  G    SG  + D+YK    +YPIVSIEDP
Sbjct: 244 GKVVIGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIEDP 303

Query: 355 FDREDWDH-TKHFSGLGI-CQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEA 414
           FD++DW+H  K  +  G   Q+VG DLL++N KR+ +AI E +CNALLLKVNQIG+VTE+
Sbjct: 304 FDQDDWEHYAKMTTECGTEVQIVGDDLLVTNPKRVAKAIAEKSCNALLLKVNQIGSVTES 363

Query: 415 IEVVNLAKDAQWAVVASHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRI 474
           IE V ++K A W V+ SHR GET+DTF+ADL+VGLSTGQIK GAPCR ERLAKYNQLLRI
Sbjct: 364 IEAVKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLLRI 423

Query: 475 EEELGDQAVYAGEDWR 481
           EEELG +A+YAG ++R
Sbjct: 424 EEELGSEAIYAGVNFR 438

BLAST of Carg12409 vs. TAIR10
Match: AT1G74030.1 (enolase 1)

HSP 1 Score: 469.5 bits (1207), Expect = 2.4e-132
Identity = 236/425 (55.53%), Postives = 307/425 (72.24%), Query Frame = 0

Query: 58  IKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKA 117
           +KARQI+DSRG PTVEVDL T+  ++ ++VPSG   G+YEA++LRDGDK  Y G  V +A
Sbjct: 54  VKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLQA 113

Query: 118 VKNINDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKE 177
           +KNIN+ ++  LIG D   Q ++D  M +LD T  K +LGANAIL VS++VCRAGA  K 
Sbjct: 114 IKNINELVAPKLIGVDVRNQADVDALMLELDGTPNKSKLGANAILGVSLSVCRAGAGAKG 173

Query: 178 VPLYKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSE 237
           VPLYKHI + +G   L++PVPAF VI+GG HAGN+LA+QE MILP+GA+ F EA +MGSE
Sbjct: 174 VPLYKHIQETSGTKELVMPVPAFNVINGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSE 233

Query: 238 TFHHLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIA 297
            +H LK +I  K+G   CN G++GGFAPN+    E L L+ +AI + GY  +IKI +D+A
Sbjct: 234 VYHTLKGIIKTKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVA 293

Query: 298 ATNFCM-GTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTK 357
           A+ F M   +YDL+FK     G +  S + + D+Y+E   D+PIVSIEDPFD++DW    
Sbjct: 294 ASEFFMKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFDQDDWSSWA 353

Query: 358 HFSGLGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQW 417
                   Q+VG DLL++N KRI  AI + +CNALLLKVNQIGTVTE+I+    +K A W
Sbjct: 354 SLQSSVDIQLVGDDLLVTNPKRIAEAIKKQSCNALLLKVNQIGTVTESIQAALDSKAAGW 413

Query: 418 AVVASHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAG 477
            V+ SHR GET+D F+ADLSVGL++GQIK GAPCR ERL+KYNQLLRIEEELG+   YAG
Sbjct: 414 GVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLSKYNQLLRIEEELGN-VRYAG 473

Query: 478 EDWRT 482
           E +R+
Sbjct: 474 EAFRS 476

BLAST of Carg12409 vs. Swiss-Prot
Match: sp|Q9ZW34|ENO3_ARATH (Cytosolic enolase 3 OS=Arabidopsis thaliana OX=3702 GN=ENO3 PE=1 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 8.0e-226
Identity = 382/472 (80.93%), Postives = 430/472 (91.10%), Query Frame = 0

Query: 9   MSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKARQILDSRG 68
           MSVQEYLDK+MLSRKIE+AVNAAVRAKT DPVLFI+NH+KKAV S+ITK+KARQILDSRG
Sbjct: 1   MSVQEYLDKHMLSRKIEDAVNAAVRAKTSDPVLFIANHLKKAVSSVITKVKARQILDSRG 60

Query: 69  IPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKNINDKISEA 128
           IPTVEVDL+TNKGVF ASVPSGD +G YEA++LRDGDKG YLGNSV KAVKNIN+KISEA
Sbjct: 61  IPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSVAKAVKNINEKISEA 120

Query: 129 LIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPLYKHIADLA 188
           LIG DP  Q +IDQAM DLD TEKK ELGANAILAVSIA C+AGAAEKEVPL KH++DL+
Sbjct: 121 LIGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAAEKEVPLCKHLSDLS 180

Query: 189 GKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFHHLKAVISE 248
           G+ N++LPVPAFTV+SGGKHA N  AIQEIMILPIGAS+FEEAL+ GSET+HHLKAVISE
Sbjct: 181 GRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGASRFEEALQWGSETYHHLKAVISE 240

Query: 249 KHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATNFCMGTKYD 308
           K+G  GCN GEDGG AP+ISS  E L+LV+EAI RTGYN++IKIAIDIAATNFC+GTKYD
Sbjct: 241 KNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTGYNDKIKIAIDIAATNFCLGTKYD 300

Query: 309 LDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSGLGICQVVG 368
           LD K+PNK GQNFKS +DMIDMYKE+C+DYPIVSIEDPFD+EDW+HTK+FS LGICQVVG
Sbjct: 301 LDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWEHTKYFSSLGICQVVG 360

Query: 369 GDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVASHRCGETD 428
            DLLMSN KR+ERAI E++CNALLLKVNQIGTVTEAIEVV +A+DAQW VV SHRCGET+
Sbjct: 361 DDLLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARDAQWGVVTSHRCGETE 420

Query: 429 DTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 481
           D+F++DLSVGL+TG IKAGAPCRGER  KYNQLLRIEEELGDQAVYAGEDW+
Sbjct: 421 DSFISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAVYAGEDWK 472

BLAST of Carg12409 vs. Swiss-Prot
Match: sp|P09104|ENOG_HUMAN (Gamma-enolase OS=Homo sapiens OX=9606 GN=ENO2 PE=1 SV=3)

HSP 1 Score: 505.4 bits (1300), Expect = 7.2e-142
Identity = 252/428 (58.88%), Postives = 321/428 (75.00%), Query Frame = 0

Query: 55  ITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSV 114
           I KI AR+ILDSRG PTVEVDLYT KG+F A+VPSG   G+YEA++LRDGDK  YLG  V
Sbjct: 3   IEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGV 62

Query: 115 TKAVKNINDKISEALI--GRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAG 174
            KAV +IN  I+ ALI  G    +Q ++D  M +LD TE K + GANAIL VS+AVC+AG
Sbjct: 63  LKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAG 122

Query: 175 AAEKEVPLYKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEAL 234
           AAE+E+PLY+HIA LAG ++LILPVPAF VI+GG HAGN LA+QE MILP+GA  F +A+
Sbjct: 123 AAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAM 182

Query: 235 KMGSETFHHLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKI 294
           ++G+E +H LK VI +K+G    N G++GGFAPNI   +EAL+LV+EAI + GY E+I I
Sbjct: 183 RLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTEKIVI 242

Query: 295 AIDIAATNFCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDW 354
            +D+AA+ F    KYDLDFK+P      + +G  +  +Y++   DYP+VSIEDPFD++DW
Sbjct: 243 GMDVAASEFYRDGKYDLDFKSPTD-PSRYITGDQLGALYQDFVRDYPVVSIEDPFDQDDW 302

Query: 355 DHTKHFSGLGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAK 414
                F+     Q+VG DL ++N KRIERA++E  CN LLLKVNQIG+VTEAI+   LA+
Sbjct: 303 AAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQ 362

Query: 415 DAQWAVVASHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQA 474
           +  W V+ SHR GET+DTF+ADL VGL TGQIK GAPCR ERLAKYNQL+RIEEELGD+A
Sbjct: 363 ENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEA 422

Query: 475 VYAGEDWR 481
            +AG ++R
Sbjct: 423 RFAGHNFR 429

BLAST of Carg12409 vs. Swiss-Prot
Match: sp|Q27527|ENO_CAEEL (Enolase OS=Caenorhabditis elegans OX=6239 GN=enol-1 PE=1 SV=3)

HSP 1 Score: 505.0 bits (1299), Expect = 9.3e-142
Identity = 253/428 (59.11%), Postives = 315/428 (73.60%), Query Frame = 0

Query: 55  ITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSV 114
           ITKI ARQI DSRG PTVEVDL+T KGVF A+VPSG   G++EA++LRDGDK  +LG  V
Sbjct: 3   ITKIHARQIYDSRGNPTVEVDLFTEKGVFRAAVPSGASTGVHEALELRDGDKAVHLGKGV 62

Query: 115 TKAVKNINDKISEALI--GRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAG 174
            KAV NIN+KI+ ALI  G D T Q +ID  M  LD +E KG LGANAIL VS+AV +AG
Sbjct: 63  LKAVSNINEKIAPALIAKGFDVTAQKDIDDFMMALDGSENKGNLGANAILGVSLAVAKAG 122

Query: 175 AAEKEVPLYKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEAL 234
           A  K +PLYK+IA+LAG   ++LPVPAF VI+GG HAGN LA+QE MILP+GAS F EA+
Sbjct: 123 AVHKGLPLYKYIAELAGTGKVVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFAEAM 182

Query: 235 KMGSETFHHLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKI 294
           +MGSE +HHLKA I +++G      G++GGFAPNI    E LDL+  AI + GY  +I I
Sbjct: 183 RMGSEVYHHLKAEIKKRYGLDATAVGDEGGFAPNIQDNKEGLDLLNTAIDKAGYTGKISI 242

Query: 295 AIDIAATNFCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDW 354
            +D+AA+ F    KYDLDFK P      + SG+ + ++Y+    +YP+VSIED FD++DW
Sbjct: 243 GMDVAASEFFKDGKYDLDFKNPASDSSKWLSGEQLTELYQSFIKEYPVVSIEDAFDQDDW 302

Query: 355 DHTKHFSGLGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAK 414
           D+   F G    Q+VG DL ++N KRI+ AID+ +CN LLLKVNQIG+VTE+IE   L++
Sbjct: 303 DNWGKFHGATSIQLVGDDLTVTNPKRIQTAIDKKSCNCLLLKVNQIGSVTESIEAAKLSR 362

Query: 415 DAQWAVVASHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQA 474
              W V+ SHR GET+DTF+ADL VGL+TGQIK GAPCR ERLAKYNQLLRIEEELG  A
Sbjct: 363 ANGWGVMVSHRSGETEDTFIADLVVGLATGQIKTGAPCRSERLAKYNQLLRIEEELGADA 422

Query: 475 VYAGEDWR 481
           VYAG ++R
Sbjct: 423 VYAGHNFR 430

BLAST of Carg12409 vs. Swiss-Prot
Match: sp|P17183|ENOG_MOUSE (Gamma-enolase OS=Mus musculus OX=10090 GN=Eno2 PE=1 SV=2)

HSP 1 Score: 501.1 bits (1289), Expect = 1.3e-140
Identity = 249/428 (58.18%), Postives = 322/428 (75.23%), Query Frame = 0

Query: 55  ITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSV 114
           I KI AR+ILDSRG PTVEVDLYT KG+F A+VPSG   G+YEA++LRDGDK  YLG  V
Sbjct: 3   IEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGV 62

Query: 115 TKAVKNINDKISEALI--GRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAG 174
            KAV +IN +I+ ALI  G    +Q ++D  M +LD TE K + GANAIL VS+AVC+AG
Sbjct: 63  LKAVDHINSRIAPALISSGISVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAG 122

Query: 175 AAEKEVPLYKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEAL 234
           AAE+++PLY+HIA LAG ++LILPVPAF VI+GG HAGN LA+QE MILP+GA  F +A+
Sbjct: 123 AAERDLPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAM 182

Query: 235 KMGSETFHHLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKI 294
           ++G+E +H LK VI +K+G    N G++GGFAPNI   +EAL+LV+EAI + GY E++ I
Sbjct: 183 RLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTEKMVI 242

Query: 295 AIDIAATNFCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDW 354
            +D+AA+ F    KYDLDFK+P      + +G  +  +Y++   +YP+VSIEDPFD++DW
Sbjct: 243 GMDVAASEFYRDGKYDLDFKSPAD-PSRYITGDQLGALYQDFVRNYPVVSIEDPFDQDDW 302

Query: 355 DHTKHFSGLGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAK 414
                F+     Q+VG DL ++N KRIERA++E  CN LLLKVNQIG+VTEAI+   LA+
Sbjct: 303 AAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQ 362

Query: 415 DAQWAVVASHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQA 474
           +  W V+ SHR GET+DTF+ADL VGL TGQIK GAPCR ERLAKYNQL+RIEEELGD+A
Sbjct: 363 ENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEA 422

Query: 475 VYAGEDWR 481
            +AG ++R
Sbjct: 423 RFAGHNFR 429

BLAST of Carg12409 vs. Swiss-Prot
Match: sp|O57391|ENOG_CHICK (Gamma-enolase OS=Gallus gallus OX=9031 GN=ENO2 PE=2 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.0e-140
Identity = 243/428 (56.78%), Postives = 323/428 (75.47%), Query Frame = 0

Query: 55  ITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSV 114
           + +I AR+ILDSRG PTVEVDLYT+KG+F A+VPSG   G+YEA++LRD DK  +LG  V
Sbjct: 3   VERIHAREILDSRGNPTVEVDLYTHKGMFRAAVPSGASTGIYEALELRDNDKSRFLGKGV 62

Query: 115 TKAVKNINDKISEALIGR--DPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAG 174
            +AV +IN  ++ A++G       Q +ID  M ++D TE K + GANAIL VS+AVC+AG
Sbjct: 63  LQAVDHINSTVAPAIVGSGLSVVDQEKIDNLMLEMDGTENKSKFGANAILGVSLAVCKAG 122

Query: 175 AAEKEVPLYKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEAL 234
           AAEK+VPLY+HIADLAG ++LILPVPAF VI+GG HAGN LA+QE MILP+GA  F +A+
Sbjct: 123 AAEKDVPLYRHIADLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAM 182

Query: 235 KMGSETFHHLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKI 294
           ++G+E +H+LK+VI EK+G    N G++GGFAPNI   +EAL+L++EAI + GY ++I I
Sbjct: 183 RIGAEVYHNLKSVIKEKYGKDATNVGDEGGFAPNILENSEALELLKEAIDKAGYTDKIVI 242

Query: 295 AIDIAATNFCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDW 354
            +D+AA+ F    KYDLDFK+P+     + S  ++ D+Y+     YP++SIEDPFD++DW
Sbjct: 243 GMDVAASEFYRDGKYDLDFKSPDD-PSRYISADELGDLYQSFVRAYPVLSIEDPFDQDDW 302

Query: 355 DHTKHFSGLGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAK 414
           +    F+     Q+VG DL ++N KRIERA++E  CN LLLKVNQIG+VTEAI+   LA+
Sbjct: 303 EAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQ 362

Query: 415 DAQWAVVASHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQA 474
           +  W V+ SHR GET+DTF+ADL V L TGQIK GAPCR ERLAKYNQL+RIEEELGD+A
Sbjct: 363 ENGWGVMVSHRSGETEDTFIADLVVALCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEA 422

Query: 475 VYAGEDWR 481
            +AG ++R
Sbjct: 423 RFAGHNFR 429

BLAST of Carg12409 vs. TrEMBL
Match: tr|A0A0A0M0P5|A0A0A0M0P5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G528460 PE=3 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 5.0e-265
Identity = 461/480 (96.04%), Postives = 470/480 (97.92%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKA+PS+ITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAV+LRDGDKGTYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 121 INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           IN+KISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLAGKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300
           HLKAVI+EKHGAHGCNFGEDGGFAPNISSF EALDLV+EAI R GYNERIKIAID+AATN
Sbjct: 241 HLKAVITEKHGAHGCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360
           FCMGTKYDLDFKAPNK  QNFKSGKDMIDMYKELC DYPIVSIEDPFDREDWDHTKHFS 
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420
           LGICQVVGGDLLMSNKKRIERAIDE TCNALLLKVNQIGTVTEAIEVV LAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLADLSVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQA+YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

BLAST of Carg12409 vs. TrEMBL
Match: tr|A0A1S3C645|A0A1S3C645_CUCME (cytosolic enolase 3 OS=Cucumis melo OX=3656 GN=LOC103497104 PE=3 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 3.2e-264
Identity = 460/480 (95.83%), Postives = 469/480 (97.71%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKA+PS+ITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAV+LRDGDKGTYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 121 INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           IN+KISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLAGKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300
           HLKAVI+EKHGAH CNFGEDGGFAPNISSF EALDLV+EAI R GYNERIKIAID+AATN
Sbjct: 241 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360
           FCMGTKYDLDFKAPNK  QNFKSGKDMIDMYKELC DYPIVSIEDPFDREDWDHTKHFS 
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420
           LGICQVVGGDLLMSNKKRIERAIDE TCNALLLKVNQIGTVTEAIEVV LAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLADLSVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQA+YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

BLAST of Carg12409 vs. TrEMBL
Match: tr|A0A2P5CP35|A0A2P5CP35_9ROSA (Enolase OS=Trema orientalis OX=63057 GN=TorRG33x02_277980 PE=3 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 1.7e-236
Identity = 403/472 (85.38%), Postives = 441/472 (93.43%), Query Frame = 0

Query: 9   MSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKARQILDSRG 68
           MSVQEYLDK+MLSRKIE+AVNAAVRAKTPDPVLFISNH+KKAVPS+ITK+KARQILDSRG
Sbjct: 1   MSVQEYLDKHMLSRKIEDAVNAAVRAKTPDPVLFISNHLKKAVPSVITKVKARQILDSRG 60

Query: 69  IPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKNINDKISEA 128
           IPTVEVDL+TNKG+F AS PSGDP GMYEAV+LRDGDKG YLGNSV +AVKN+N+KISEA
Sbjct: 61  IPTVEVDLFTNKGMFRASAPSGDPTGMYEAVELRDGDKGLYLGNSVNRAVKNVNEKISEA 120

Query: 129 LIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPLYKHIADLA 188
           L+G DPT Q +IDQAM DLD TEKKGELGANAILAVS A C+AGAAEKEVPLYKHIADL+
Sbjct: 121 LVGMDPTLQSQIDQAMIDLDRTEKKGELGANAILAVSTAACKAGAAEKEVPLYKHIADLS 180

Query: 189 GKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFHHLKAVISE 248
           GKTNL LPVPAFTVISGGKHAGNNLAIQEI ILPIGAS+FEEAL+MGSET+HHLKAVI+E
Sbjct: 181 GKTNLTLPVPAFTVISGGKHAGNNLAIQEITILPIGASRFEEALQMGSETYHHLKAVITE 240

Query: 249 KHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATNFCMGTKYD 308
           K+GAHGCN GEDGGFAPNISS  E LDLV++AI RTGYNERIKIAID+AAT+FC+GTKYD
Sbjct: 241 KYGAHGCNVGEDGGFAPNISSVKEGLDLVKDAISRTGYNERIKIAIDVAATDFCIGTKYD 300

Query: 309 LDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSGLGICQVVG 368
           LDFK+PNK GQNFKSG+ MIDMYKELC +YPIVSIEDPFD+EDW+H KHFS LG+CQVVG
Sbjct: 301 LDFKSPNKSGQNFKSGEHMIDMYKELCAEYPIVSIEDPFDKEDWEHVKHFSSLGLCQVVG 360

Query: 369 GDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVASHRCGETD 428
            DLLMSN KRIERAI+E+TCNALLLK+NQIGTVTEAIEVV LAKDA W VV SHRCGET+
Sbjct: 361 DDLLMSNPKRIERAINESTCNALLLKINQIGTVTEAIEVVKLAKDANWGVVTSHRCGETE 420

Query: 429 DTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 481
           D+F+ADLSVGL+TGQIKAGAPCRGERLAKYNQLLRIEEELGD AVYAGEDWR
Sbjct: 421 DSFIADLSVGLATGQIKAGAPCRGERLAKYNQLLRIEEELGDHAVYAGEDWR 472

BLAST of Carg12409 vs. TrEMBL
Match: tr|A0A2P5DC30|A0A2P5DC30_PARAD (Enolase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_078900 PE=3 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 4.1e-235
Identity = 402/472 (85.17%), Postives = 440/472 (93.22%), Query Frame = 0

Query: 9   MSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKARQILDSRG 68
           MSVQEYLDK+MLSRKIE+AVNAAVRAKT DPVLFISNH+KKAV S+ITK+KARQILDSRG
Sbjct: 1   MSVQEYLDKHMLSRKIEDAVNAAVRAKTHDPVLFISNHLKKAVSSVITKVKARQILDSRG 60

Query: 69  IPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKNINDKISEA 128
           IPTVEVDL+TNKG+F AS PSGDP GMYEAV+LRDGDKG YLGNSV +AVKN+N+KISEA
Sbjct: 61  IPTVEVDLFTNKGMFRASAPSGDPTGMYEAVELRDGDKGLYLGNSVNRAVKNVNEKISEA 120

Query: 129 LIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPLYKHIADLA 188
           L+G DPT Q +IDQAM DLD TEKKGELGANAILAVSIA C+AGAAEKEVPLYKHIADL+
Sbjct: 121 LVGMDPTLQSQIDQAMIDLDKTEKKGELGANAILAVSIAACKAGAAEKEVPLYKHIADLS 180

Query: 189 GKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFHHLKAVISE 248
           GKTNL LPVPAFTVISGGKHAGNNLAIQEI ILPIGAS+FEEAL+MGSET+HHLKAVI+E
Sbjct: 181 GKTNLTLPVPAFTVISGGKHAGNNLAIQEITILPIGASRFEEALQMGSETYHHLKAVITE 240

Query: 249 KHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATNFCMGTKYD 308
           K+GAHGCN GEDGGFAPNISS  E LDLV+EAI RTGYNERIKIAID+AAT+FC+GTKYD
Sbjct: 241 KYGAHGCNVGEDGGFAPNISSVKEGLDLVKEAISRTGYNERIKIAIDVAATDFCIGTKYD 300

Query: 309 LDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSGLGICQVVG 368
           LDFK+PNK GQNFKSG+ MIDMYKELC +YPIVSIEDPFD+EDW+H KHFS LG+CQVVG
Sbjct: 301 LDFKSPNKSGQNFKSGEHMIDMYKELCTEYPIVSIEDPFDKEDWEHVKHFSSLGLCQVVG 360

Query: 369 GDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVASHRCGETD 428
            DLLMSN KRIERAI+E+TCNALLLK+NQIGTVTEAIEVV LAKDA W VVASHRCGET+
Sbjct: 361 DDLLMSNPKRIERAINESTCNALLLKINQIGTVTEAIEVVKLAKDANWGVVASHRCGETE 420

Query: 429 DTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 481
           D+F+ADLSVGL+TGQIKAGAPCRGERLAKYNQLL IEEELGD AVYAG+DWR
Sbjct: 421 DSFIADLSVGLATGQIKAGAPCRGERLAKYNQLLHIEEELGDHAVYAGDDWR 472

BLAST of Carg12409 vs. TrEMBL
Match: tr|A0A059AFZ2|A0A059AFZ2_EUCGR (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_J01952 PE=3 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 2.5e-232
Identity = 399/472 (84.53%), Postives = 432/472 (91.53%), Query Frame = 0

Query: 9   MSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKARQILDSRG 68
           MSVQEYLDK+MLSRKIE+AVNAAVRAKTPDPVLFISNHM+KAVPS+ITKIKARQILDSRG
Sbjct: 1   MSVQEYLDKHMLSRKIEDAVNAAVRAKTPDPVLFISNHMRKAVPSVITKIKARQILDSRG 60

Query: 69  IPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKNINDKISEA 128
           +PTVEVDLYT+KG+F AS PSGD  GMYEAV+LRDGDKG YLGNSV +AV+NIN+KISEA
Sbjct: 61  VPTVEVDLYTHKGMFRASAPSGDQTGMYEAVELRDGDKGVYLGNSVQRAVRNINEKISEA 120

Query: 129 LIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPLYKHIADLA 188
           LIG DP  Q +IDQAM DLD TEKKGELGANAILAVSIA C+AGAAEKEVPLYKHIADLA
Sbjct: 121 LIGMDPILQSQIDQAMIDLDKTEKKGELGANAILAVSIAACKAGAAEKEVPLYKHIADLA 180

Query: 189 GKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFHHLKAVISE 248
            K +L LPVP+FTVISGGKHAGNNLAIQEIMILPIGAS FEEAL+MGSET+HHLKAVI E
Sbjct: 181 CKPDLTLPVPSFTVISGGKHAGNNLAIQEIMILPIGASTFEEALQMGSETYHHLKAVIME 240

Query: 249 KHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATNFCMGTKYD 308
           KHGA GCN GEDGGFAPN+SS  EALDLV+EAI RTGYNERIKIAID+AATNFC+GTKYD
Sbjct: 241 KHGAQGCNVGEDGGFAPNVSSLREALDLVKEAISRTGYNERIKIAIDVAATNFCIGTKYD 300

Query: 309 LDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSGLGICQVVG 368
           LDFK PNK GQNFKSG+DMIDMYKELC DYPIVSIEDPFD+EDW+H K+FS LG+CQVVG
Sbjct: 301 LDFKFPNKSGQNFKSGEDMIDMYKELCSDYPIVSIEDPFDKEDWEHVKYFSSLGVCQVVG 360

Query: 369 GDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVASHRCGETD 428
            DLLMSN KR+ERAI+E+ CNA LLKVNQIGTVTEAIEVV LAKDA W +V SHRCGET+
Sbjct: 361 DDLLMSNPKRVERAINESACNAFLLKVNQIGTVTEAIEVVKLAKDAHWGIVTSHRCGETE 420

Query: 429 DTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 481
           DTF+ADLSVGL TG IKAGAPCRGERLAKYNQLLRIEEELGDQA Y GEDW+
Sbjct: 421 DTFIADLSVGLGTGHIKAGAPCRGERLAKYNQLLRIEEELGDQAGYVGEDWK 472

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022949451.11.3e-277100.00cytosolic enolase 3 [Cucurbita moschata] >XP_023525808.1 cytosolic enolase 3 [Cu... [more]
XP_022998824.11.0e-27498.96cytosolic enolase 3 [Cucurbita maxima][more]
XP_004145264.17.5e-26596.04PREDICTED: cytosolic enolase 3 [Cucumis sativus] >KGN65781.1 hypothetical protei... [more]
XP_008457408.14.9e-26495.83PREDICTED: cytosolic enolase 3 [Cucumis melo][more]
XP_022150946.11.5e-26093.79cytosolic enolase 3 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT2G29560.14.4e-22780.93cytosolic enolase[more]
AT2G36530.14.5e-13958.49Enolase[more]
AT1G74030.12.4e-13255.53enolase 1[more]
Match NameE-valueIdentityDescription
sp|Q9ZW34|ENO3_ARATH8.0e-22680.93Cytosolic enolase 3 OS=Arabidopsis thaliana OX=3702 GN=ENO3 PE=1 SV=1[more]
sp|P09104|ENOG_HUMAN7.2e-14258.88Gamma-enolase OS=Homo sapiens OX=9606 GN=ENO2 PE=1 SV=3[more]
sp|Q27527|ENO_CAEEL9.3e-14259.11Enolase OS=Caenorhabditis elegans OX=6239 GN=enol-1 PE=1 SV=3[more]
sp|P17183|ENOG_MOUSE1.3e-14058.18Gamma-enolase OS=Mus musculus OX=10090 GN=Eno2 PE=1 SV=2[more]
sp|O57391|ENOG_CHICK3.0e-14056.78Gamma-enolase OS=Gallus gallus OX=9031 GN=ENO2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A0A0M0P5|A0A0A0M0P5_CUCSA5.0e-26596.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G528460 PE=3 SV=1[more]
tr|A0A1S3C645|A0A1S3C645_CUCME3.2e-26495.83cytosolic enolase 3 OS=Cucumis melo OX=3656 GN=LOC103497104 PE=3 SV=1[more]
tr|A0A2P5CP35|A0A2P5CP35_9ROSA1.7e-23685.38Enolase OS=Trema orientalis OX=63057 GN=TorRG33x02_277980 PE=3 SV=1[more]
tr|A0A2P5DC30|A0A2P5DC30_PARAD4.1e-23585.17Enolase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_078900 PE=3 SV=1[more]
tr|A0A059AFZ2|A0A059AFZ2_EUCGR2.5e-23284.53Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_J01952 PE=3 SV... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0000287magnesium ion binding
GO:0004634phosphopyruvate hydratase activity
Vocabulary: Cellular Component
TermDefinition
GO:0000015phosphopyruvate hydratase complex
Vocabulary: Biological Process
TermDefinition
GO:0006096glycolytic process
Vocabulary: INTERPRO
TermDefinition
IPR020809Enolase_CS
IPR036849Enolase-like_C_sf
IPR029017Enolase-like_N
IPR020810Enolase_C
IPR020811Enolase_N
IPR000941Enolase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006096 glycolytic process
biological_process GO:0009094 L-phenylalanine biosynthetic process
biological_process GO:0000162 tryptophan biosynthetic process
biological_process GO:0006571 tyrosine biosynthetic process
cellular_component GO:0000015 phosphopyruvate hydratase complex
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004634 phosphopyruvate hydratase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12409-RACarg12409-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000941EnolasePRINTSPR00148ENOLASEcoord: 214..227
score: 59.31
coord: 391..405
score: 76.87
coord: 420..437
score: 67.76
coord: 87..101
score: 56.06
coord: 368..379
score: 48.11
coord: 157..173
score: 75.58
IPR000941EnolaseTIGRFAMTIGR01060TIGR01060coord: 55..478
e-value: 1.7E-149
score: 496.1
IPR000941EnolasePANTHERPTHR11902ENOLASEcoord: 9..481
IPR000941EnolaseHAMAPMF_00318Enolasecoord: 53..468
score: 39.409
IPR000941EnolaseCDDcd03313enolasecoord: 57..467
e-value: 0.0
score: 640.684
IPR020811Enolase, N-terminalSMARTSM01193Enolase_N_3coord: 55..184
e-value: 8.0E-76
score: 267.9
IPR020811Enolase, N-terminalPFAMPF03952Enolase_Ncoord: 55..184
e-value: 3.1E-53
score: 179.3
IPR020810Enolase, C-terminal TIM barrel domainSMARTSM01192Enolase_C_3coord: 192..482
e-value: 1.9E-162
score: 555.7
IPR020810Enolase, C-terminal TIM barrel domainPFAMPF00113Enolase_Ccoord: 193..480
e-value: 1.4E-121
score: 405.3
IPR029017Enolase-like, N-terminalGENE3DG3DSA:3.30.390.10coord: 50..177
e-value: 3.6E-54
score: 184.4
IPR036849Enolase-like, C-terminal domain superfamilyGENE3DG3DSA:3.20.20.120coord: 178..483
e-value: 1.2E-132
score: 443.5
IPR036849Enolase-like, C-terminal domain superfamilySUPERFAMILYSSF51604Enolase C-terminal domain-likecoord: 193..480
NoneNo IPR availablePANTHERPTHR11902:SF13CYTOSOLIC ENOLASE 3coord: 9..481
NoneNo IPR availableSUPERFAMILYSSF54826Enolase N-terminal domain-likecoord: 55..188
IPR020809Enolase, conserved sitePROSITEPS00164ENOLASEcoord: 391..404

The following gene(s) are paralogous to this gene:

None