Carg12392 (gene) Silver-seed gourd

NameCarg12392
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionMethylsterol monooxygenase 2-1
LocationCucurbita_argyrosperma_scaffold_074 : 716074 .. 719858 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCTCTTCTTGAATCTGCCTGGCGGGTACTGCTTTTTCTCTTCATAATCTTGTTCTTTCCGCCCTAGATTTGATCTTCATTTTCAAGCACCGATTAGATTCTAAACTTCTGCGAGTAGTTTTTTTTTTTTTTAATTAATTTATTTATTTCTTACGTTCGTTTTCGAGTAGGTTTTGATGTGGGAGTGTTGTCCTTGAATTTCCCTCTCTTATTTGTTAATCGATTGTTGACGTGAGAGTGACATTGCGGTTTTTTATGTAATTTTAGGTTGAATTTCATCTTCTATAGTTGATTTGAAATAGGATCTTGATGTTGAGAGCTAGTGATTTGCACGTTTGATGTAATACGATGAGGTTATGGGTGCCTTGTTTTCTTAAGATTTTTTGTTTGTTTTCCAATTGGAGGATTCAATTTTGGTTTCGTTTGAATGAGTGTTTCCTTTATTTGAAAACATTGACTTGCTTGAGTGTAGAATTACACTATGGTTCTTATTAGTAGAATTAAACTGTGTTTCTTTTTGTATTTGGTGCTTCTTTGATGGGTTCTTTTGCTCTATGGTGCAGTATTTGATTACGAATTTTAGTGATTTTCAACTCGCTTGTGTTGGAAGCTTCATAATCCATGAAAGCGTTTTCTTCTTATCTGGGCTTCCCTTTATTTTTCTCGAAAAAGCAGGATGGCTGAGCAAGTACAAGATCCAGGTTTGCTTTTTCACTTCACCACAAATTTCATAACCAATGAATATTTAGTCAATATTTGATACTTGAATTAGATTTACCTTCGTTAGTGATTCATGTGTGATTATGAACTAAATTAGCAGTTAGCAGCACTTTTTCTGGAAAGATTCGAAGTTATAGGTTTGATATGGAACAACTTAGTATTTCTTTATTCATTCTCATCCAATTAAAAAAGTAGGATGAGTTGATTTCTTGATCTTGATTAATTGTGAGATTAAAAAAAATTATTGGTCTAGGCTTTAATATCAGCCTTACTTTTAAGACGAAAATGCAGCACTTTAACTAGTTCTAATTTTAATTATCCTTATCTTTTGATCTCCAATGGAGAAGATTACAGGAAACAATGATTAATTTTTTTCTTTCCCGCTTTTTGATAATGAACAATTTCATTTATATATGAAATTTACAAAAAGGATAATATCCATGGTTACAAAAGGCTTTCAGAATTGATTTACATGATATTGCCAAGTCAAAAAAAAAATTGAAGGGGGCCTCCTTGTTTGTAAAGACTGTTAGATTCCCCTCCCCCACCCCACAAAAGGAAAAAAAAAAAAAAAAAGATGCCCTAGAAATGTTCTTCTAAGCAGAGCTTTCGTGTTTCGAAAAGGGTAGTTTTGGTCAAACATGTTGGGTAAAAGCAATTTTGTGGAAAGTGAGCCACTCAGATTAGGAAGCCAAATCCATGAATCCTCTGTTGAGCAAGATAGCCACTCGGAAAAAACACGCACAACAATCTTGCACGGGCAGGTGGTGAGACTAGTTGGTTGGTAATCAGCCACTGCTCAGGCTTCAAGCTTCTTTGGGATGAGGCAAATGTAAATTTTATTGAGATTTGTGTTTATGGTTCCATTATGGAAAAAAATATTGGAACACTCTCATAATATCAATTCTTAGAATGTTCCAACATTTTTTTTTAAAGAATTCGGTGGCAGTACCATCTAGGCCAGATGATTTGTCTGTTCCAAGAAGCTGTACTCCCTTGTATACTTCAAGTTCGGTGAAACGAGCTTCTAAATCAGTAGCCAAAATGACATCAATCGGACTCCAATTTATGATAGTGGGAAAAGTCTCCAGTTTACACTTTTTTGTGTGTAAAGCTTTGTAAAAAGCCAAGAATTCCGGAACTATGTCATCCCTGTTAAGCACTCGTCTACCAACAACGGAGAGAATCTCAAGTATGGTGTTCTTCCTTTCCTTTGCAGACATAAACAATGAAAGAATCTGGTGTTAAAGCACCTCTTCTATCTATCTTCTCTTACATTGCTGCTTTCAGCTTTGTTCCTCCTTTGCAGCCAAGGACAATAATTCAGCTTCCATCAATTTCCTATGCGTATGTTGAGCTATGGAAAAAAGAAAAAAGAGATGAAAAGGGAAAGGAACTGTTTCTCATTGATTGCCTTATAGACGATACTGCTGATACTTAAGGATGCAGACAAAGTACCTATCAACTAAGTTTTCCAGCTCAATAATTACACCTCAATTAAGTTAGGGAGACTTTTGAGATGATATCCGTCCGAATGAGATAAGAGAATTCCAATTCTGTAACTATTCTTTTTTCTTCTCAACATTGAAGAAAGAATGAAAAAAGGTAACAACAAGTAAAAAAATCAGAATCCCCAGATTGGTAAAATCATGCCATCCTTCCCTAGGGAACGTTGCCCCTCGCTACTGCGTTCTGTTAGGCCATTGATTATAAGAACAAATGTAGTTTTAACCCACTCTTAATTTTGGATGAATTGTTTTTAGTTGTGATATAAGTCTTTTCTGCTTTTTGCAGGCAAAGACTAATAGTTCTGCTGCACAAGGAAAATGTATTTCACGTCTATTGCTGTATCATTTTGGTGTAAATCTGCCAGTTATGATTGTTTCTTATCCTGTCTTCAAGCTTATGGGAATGAGAAGCACTCTTCCATTGCCATCCTGGTATCTTCTGGTTCTTTATGTCCTAAACCATATATCTTAGGTGTTTAATCCTTCTGGTTTAGTTTACTTTCATTGTTTTCTTATCTTGGATCATAGGATTTAATTTAGTTTGGGTTTCATTTAATGTAGGAAAGTAGTTTTCGGCCAGATAATATTCTACTTTATCGTGGAGGATTTTGTTTTCTACTGGGGGCATAGAATTTTGCACACCAAATGGCTGTACCAGAATGTGCACAGCGTTCATCACGAGTGAGTGAGATTTATAAACGAAAATGAATGATTATTCTGTGTTTTCCCATGATAAAATTGTCCTAGGCTGTTCTAAGTTGCTTATCAAGTGCAGATATGCTACCCCTTTTGGACTAACATCAGAATATGCTCACCCTGCTGAGATCCTGTTCCTTGGATTTGCTACCATTGTCGGTCCGGCTCTTACCGGCCCCCATCTAATGACTCTGTGGCTATGGATGGTTCTTAGAGTGCTCGAGACTGTCGAGGCTCATTGTGGCTATCATTTTCCGTGGAGCCCTTCAAACTTCATACCTTTATATGGGGGGTGAGTCATGTTTTAACAAGAATACGTTGTACTATGTCTGTTGTTCCTGAAATCGGCTTCTTTGCTCGTGCTCTACAGTGCTTACTTCCATGATTATCATCACCGTCTGCTTTTCACAAAATCTGGCAACTACTCGTCGACATTCACTTACATGGACTGGTGAGTTCTGGTCCAAGAAGGGTCGTTTGAACTTCATAGCATTAAAACCAGCTTTGTGGAACACTGATTTACTTCACTTCAATAGATATCGAAAACTATTATTGACAAAGAAAACTATTACTTGCAGGATATTTGGAACGGACAAGGGGTTTAGAAATTTGGAAGCTATAAAAAAGGCAGAAAGTTGATGGGCTGATAGAGAGCTGAGGAAAACGTCGTTGGGTCGAGTCGAGTTGGGTACATTCGAGATAGGTTTCTGATGTATAGGCACATTTGAAAAGGTTTAGTTCATATCACTCTTTCTTGTGTGCCATGATGTGTTTTATGTCTTCTTTTTGTTGTTTAAATTTGAGTATTCTTGAAGTAATGAAGAGCTATGGAACCTTAATCATCCTTCAAATTTTA

mRNA sequence

ATGGCTGCTCTTCTTGAATCTGCCTGGCGGTATTTGATTACGAATTTTAGTGATTTTCAACTCGCTTGTGTTGGAAGCTTCATAATCCATGAAAGCGTTTTCTTCTTATCTGGGCTTCCCTTTATTTTTCTCGAAAAAGCAGGATGGCTGAGCAAGTACAAGATCCAGGCAAAGACTAATAGTTCTGCTGCACAAGGAAAATGTATTTCACGTCTATTGCTGTATCATTTTGGTGTAAATCTGCCAGTTATGATTGTTTCTTATCCTGTCTTCAAGCTTATGGGAATGAGAAGCACTCTTCCATTGCCATCCTGGAAAGTAGTTTTCGGCCAGATAATATTCTACTTTATCGTGGAGGATTTTGTTTTCTACTGGGGGCATAGAATTTTGCACACCAAATGGCTGTACCAGAATGTGCACAGCGTTCATCACGAATATGCTACCCCTTTTGGACTAACATCAGAATATGCTCACCCTGCTGAGATCCTGTTCCTTGGATTTGCTACCATTGTCGGTCCGGCTCTTACCGGCCCCCATCTAATGACTCTGTGGCTATGGATGGTTCTTAGAGTGCTCGAGACTGTCGAGGCTCATTGTGGCTATCATTTTCCGTGGAGCCCTTCAAACTTCATACCTTTATATGGGGGTGCTTACTTCCATGATTATCATCACCGTCTGCTTTTCACAAAATCTGGCAACTACTCGTCGACATTCACTTACATGGACTGGATATTTGGAACGGACAAGGGGTTTAGAAATTTGGAAGCTATAAAAAAGGCAGAAAGTTGATGGGCTGATAGAGAGCTGAGGAAAACGTCGTTGGGTCGAGTCGAGTTGGGTACATTCGAGATAGGTTTCTGATGTATAGGCACATTTGAAAAGGTTTAGTTCATATCACTCTTTCTTGTGTGCCATGATGTGTTTTATGTCTTCTTTTTGTTGTTTAAATTTGAGTATTCTTGAAGTAATGAAGAGCTATGGAACCTTAATCATCCTTCAAATTTTA

Coding sequence (CDS)

ATGGCTGCTCTTCTTGAATCTGCCTGGCGGTATTTGATTACGAATTTTAGTGATTTTCAACTCGCTTGTGTTGGAAGCTTCATAATCCATGAAAGCGTTTTCTTCTTATCTGGGCTTCCCTTTATTTTTCTCGAAAAAGCAGGATGGCTGAGCAAGTACAAGATCCAGGCAAAGACTAATAGTTCTGCTGCACAAGGAAAATGTATTTCACGTCTATTGCTGTATCATTTTGGTGTAAATCTGCCAGTTATGATTGTTTCTTATCCTGTCTTCAAGCTTATGGGAATGAGAAGCACTCTTCCATTGCCATCCTGGAAAGTAGTTTTCGGCCAGATAATATTCTACTTTATCGTGGAGGATTTTGTTTTCTACTGGGGGCATAGAATTTTGCACACCAAATGGCTGTACCAGAATGTGCACAGCGTTCATCACGAATATGCTACCCCTTTTGGACTAACATCAGAATATGCTCACCCTGCTGAGATCCTGTTCCTTGGATTTGCTACCATTGTCGGTCCGGCTCTTACCGGCCCCCATCTAATGACTCTGTGGCTATGGATGGTTCTTAGAGTGCTCGAGACTGTCGAGGCTCATTGTGGCTATCATTTTCCGTGGAGCCCTTCAAACTTCATACCTTTATATGGGGGTGCTTACTTCCATGATTATCATCACCGTCTGCTTTTCACAAAATCTGGCAACTACTCGTCGACATTCACTTACATGGACTGGATATTTGGAACGGACAAGGGGTTTAGAAATTTGGAAGCTATAAAAAAGGCAGAAAGTTGA

Protein sequence

MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTNSSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTYMDWIFGTDKGFRNLEAIKKAES
BLAST of Carg12392 vs. NCBI nr
Match: XP_022948631.1 (methylsterol monooxygenase 2-2-like [Cucurbita moschata])

HSP 1 Score: 547.7 bits (1410), Expect = 2.1e-152
Identity = 262/262 (100.00%), Postives = 262/262 (100.00%), Query Frame = 0

Query: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60
           MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN
Sbjct: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60

Query: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120
           SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED
Sbjct: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120

Query: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180
           FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL
Sbjct: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240
           MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY
Sbjct: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAES 263
           MDWIFGTDKGFRNLEAIKKAES
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAES 262

BLAST of Carg12392 vs. NCBI nr
Match: XP_022998327.1 (methylsterol monooxygenase 2-2-like [Cucurbita maxima])

HSP 1 Score: 545.8 bits (1405), Expect = 7.9e-152
Identity = 261/262 (99.62%), Postives = 261/262 (99.62%), Query Frame = 0

Query: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60
           MAALLESAWRYLITNFSDFQLAC GSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN
Sbjct: 1   MAALLESAWRYLITNFSDFQLACFGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60

Query: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120
           SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED
Sbjct: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120

Query: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180
           FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL
Sbjct: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240
           MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY
Sbjct: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAES 263
           MDWIFGTDKGFRNLEAIKKAES
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAES 262

BLAST of Carg12392 vs. NCBI nr
Match: XP_023521502.1 (methylsterol monooxygenase 2-2-like [Cucurbita pepo subsp. pepo] >XP_023523161.1 methylsterol monooxygenase 2-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 542.3 bits (1396), Expect = 8.8e-151
Identity = 259/261 (99.23%), Postives = 260/261 (99.62%), Query Frame = 0

Query: 2   AALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTNS 61
           AALLESAW+YLITNFSDFQLAC GSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTNS
Sbjct: 3   AALLESAWQYLITNFSDFQLACFGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTNS 62

Query: 62  SAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVEDF 121
           SAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVEDF
Sbjct: 63  SAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVEDF 122

Query: 122 VFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLM 181
           VFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLM
Sbjct: 123 VFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLM 182

Query: 182 TLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTYM 241
           TLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTYM
Sbjct: 183 TLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTYM 242

Query: 242 DWIFGTDKGFRNLEAIKKAES 263
           DWIFGTDKGFRNLEAIKKAES
Sbjct: 243 DWIFGTDKGFRNLEAIKKAES 263

BLAST of Carg12392 vs. NCBI nr
Match: XP_022964698.1 (methylsterol monooxygenase 2-2 [Cucurbita moschata] >XP_022970560.1 methylsterol monooxygenase 2-2 isoform X2 [Cucurbita maxima] >XP_023521038.1 methylsterol monooxygenase 2-2-like [Cucurbita pepo subsp. pepo] >XP_023519298.1 methylsterol monooxygenase 2-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 531.6 bits (1368), Expect = 1.5e-147
Identity = 252/262 (96.18%), Postives = 257/262 (98.09%), Query Frame = 0

Query: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60
           MA LLESAWRYLITNFSDFQLAC+GSFIIHESVFFLSGLPF+FLE+AGWLSKYKIQAK N
Sbjct: 1   MAELLESAWRYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNN 60

Query: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120
           S AAQGKCISRLLLYHFGVNLPVMIVSYPVFK MGMRSTLPLPSWKVVFGQIIFYFIVED
Sbjct: 61  SPAAQGKCISRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVED 120

Query: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180
           FVFYWGHRILHTKWLY+NVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240
           MTLWLWMVLRVLETVEAHCGY FPWSPSNFIPLYGGAYFHDYHHRLL+TKSGNYSSTFTY
Sbjct: 181 MTLWLWMVLRVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAES 263
           MDWIFGTDKGFRNLEAIKKAES
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAES 262

BLAST of Carg12392 vs. NCBI nr
Match: XP_008457442.1 (PREDICTED: methylsterol monooxygenase 2-2 [Cucumis melo])

HSP 1 Score: 528.1 bits (1359), Expect = 1.7e-146
Identity = 248/262 (94.66%), Postives = 257/262 (98.09%), Query Frame = 0

Query: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60
           MAALLESAW+YLITNFSDFQLAC+GSFIIHESVFFLSGLPFI LE+AGWLSKYKIQAK N
Sbjct: 1   MAALLESAWQYLITNFSDFQLACIGSFIIHESVFFLSGLPFILLERAGWLSKYKIQAKNN 60

Query: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120
           S AAQGKCISRLLLYHFGVNLPVM+VSYPVFK MGMRSTLPLPSWKVVFGQIIFYFI+ED
Sbjct: 61  SPAAQGKCISRLLLYHFGVNLPVMLVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180
           FVFYWGHRILHTKWLY+NVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240
            TLWLWMV+RVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLL+TKSGNYSSTFTY
Sbjct: 181 FTLWLWMVVRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAES 263
           MDWIFGTDKGFRNLEAIKKAE+
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAEN 262

BLAST of Carg12392 vs. TAIR10
Match: AT1G07420.1 (sterol 4-alpha-methyl-oxidase 2-1)

HSP 1 Score: 470.7 bits (1210), Expect = 5.9e-133
Identity = 214/259 (82.63%), Postives = 241/259 (93.05%), Query Frame = 0

Query: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60
           MA+ +ES W+YL+T+FSDFQLAC+GSF++HESVFFLSGLPFIFLE+ G+LSKYKIQ K N
Sbjct: 1   MASFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLERQGFLSKYKIQTKNN 60

Query: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120
           + AAQGKCI+RLLLYHF VNLP+M+ SYPVF+ MGMRS+ PLPSWK V  QI+FYFI+ED
Sbjct: 61  TPAAQGKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180
           FVFYWGHRILH+KWLY+NVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL
Sbjct: 121 FVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240
           +TLWLWMVLRVLETVEAHCGYHFPWS SNF+PLYGGA FHDYHHRLL+TKSGNYSSTF Y
Sbjct: 181 ITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVY 240

Query: 241 MDWIFGTDKGFRNLEAIKK 260
           MDWIFGTDKG+R L+ +K+
Sbjct: 241 MDWIFGTDKGYRRLKTLKE 259

BLAST of Carg12392 vs. TAIR10
Match: AT2G29390.1 (sterol 4-alpha-methyl-oxidase 2-2)

HSP 1 Score: 459.1 bits (1180), Expect = 1.8e-129
Identity = 212/271 (78.23%), Postives = 241/271 (88.93%), Query Frame = 0

Query: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60
           M +L+ES W+YL+T+FSDFQLAC+GSFI+HESVFFLSGLP+IFLE+ G+LS YKIQ K+N
Sbjct: 1   MDSLVESGWKYLVTHFSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSN 60

Query: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120
           +  AQGKCI+RLLLYH  VNLP+M+ SYPVF+ MGM S+ PLPSWKVV  QI+FYFI+ED
Sbjct: 61  TPEAQGKCIARLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180
           FVFYWGHRILHTKWLY+NVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGG------------AYFHDYHHRLLF 240
           +TLWLWM+LRV+ETVEAHCGYHFPWSPSNF+PLYGG            A FHDYHHRLL+
Sbjct: 181 ITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLY 240

Query: 241 TKSGNYSSTFTYMDWIFGTDKGFRNLEAIKK 260
           TKSGNYSSTF YMDWIFGTDKG+R L+A+K+
Sbjct: 241 TKSGNYSSTFVYMDWIFGTDKGYRKLKALKE 271

BLAST of Carg12392 vs. TAIR10
Match: AT4G12110.1 (sterol-4alpha-methyl oxidase 1-1)

HSP 1 Score: 228.4 bits (581), Expect = 5.1e-60
Identity = 115/265 (43.40%), Postives = 160/265 (60.38%), Query Frame = 0

Query: 5   LESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKA----GWLSKYKIQAKTN 64
           LE+ W       SD+ L C     +   VF L  LP +F+E A    G  ++YKIQ K N
Sbjct: 21  LETLWFDYSATKSDYYLYCHNILFLF-LVFSLVPLPLVFVELARSASGLFNRYKIQPKVN 80

Query: 65  SSAA-QGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVE 124
            S +   KC   ++     V  P+ +VSYP  +++ +RS LPLP+   +  Q++ YF++E
Sbjct: 81  YSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGLPLPTITEMLSQLVVYFLIE 140

Query: 125 DFVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPH 184
           D+  YW HR  H+KW Y  +H VHHEY  P G  + YAH AE+L LG  T +GPA+   H
Sbjct: 141 DYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPTFMGPAIAPGH 200

Query: 185 LMTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFT 244
           ++T WLW+ LR +E +E H GY FPWSP+ +IP YGGA +HDYHH +      N++S FT
Sbjct: 201 MITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFT 260

Query: 245 YMDWIFGTDKGFR----NLEAIKKA 261
           Y D+I+GTDKG+R     LE IK++
Sbjct: 261 YCDYIYGTDKGYRFQKKLLEQIKES 284

BLAST of Carg12392 vs. TAIR10
Match: AT4G22753.1 (sterol 4-alpha methyl oxidase 1-3)

HSP 1 Score: 223.4 bits (568), Expect = 1.6e-58
Identity = 106/248 (42.74%), Postives = 150/248 (60.48%), Query Frame = 0

Query: 6   ESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTNSSAA- 65
           E+ W       S+F + C  + ++   VF L+  P + +E  GW  ++KIQ K   S + 
Sbjct: 22  ETVWFDYSATKSNFHVYC-HTILVLFLVFSLAPFPLVIVEWTGWFDQFKIQKKVKYSLSD 81

Query: 66  QGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFY 125
             +C   ++     V   + IVSYP  +++G+RS LPLPS   +  Q++ YF++ED+  Y
Sbjct: 82  MFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPLPSLMEIVAQLVVYFLIEDYTNY 141

Query: 126 WGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLW 185
           W HR +H KW Y+ +H +HHEY +P G  S YAH AEIL LG  T +GPA+   H+MT W
Sbjct: 142 WIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEILILGIPTFLGPAIAPGHIMTFW 201

Query: 186 LWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTYMDWI 245
           LW+ LR  E +E H GY FPWS +  IP YGG  +HDYHH +      N++S FTY D+I
Sbjct: 202 LWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDYHHYVGGQSQSNFASVFTYCDYI 261

Query: 246 FGTDKGFR 253
           +GTDKG+R
Sbjct: 262 YGTDKGYR 268

BLAST of Carg12392 vs. TAIR10
Match: AT4G22756.1 (sterol C4-methyl oxidase 1-2)

HSP 1 Score: 221.5 bits (563), Expect = 6.2e-58
Identity = 108/253 (42.69%), Postives = 152/253 (60.08%), Query Frame = 0

Query: 5   LESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEK----AGWLSKYKIQAKT- 64
           LE+ W       SD+ L C     +   +F L  LP +F+E     +   ++YKIQ K  
Sbjct: 21  LETLWFDYSATKSDYYLYCHNILFLF-LIFSLVPLPLVFIESSQSTSDLFNRYKIQPKVK 80

Query: 65  NSSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVE 124
           NS ++  KC   ++     V  P+ +VSYP  +++ +RS LPLPS   +  Q++ YF+VE
Sbjct: 81  NSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQMIEIRSGLPLPSCMEIVAQLVVYFLVE 140

Query: 125 DFVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPH 184
           D+  YW HR  H KW Y+  H +HHEY  P G  + YAH AE+L LG  T +GPA+   H
Sbjct: 141 DYTNYWVHRFFHCKWGYEKFHHIHHEYTAPIGYAAPYAHWAEVLLLGIPTFLGPAIAPGH 200

Query: 185 LMTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFT 244
           ++T WLW+ LR +E +E H GY FPWS + +IP YGGA +HDYHH +      N++S FT
Sbjct: 201 MITFWLWIALRQIEAIETHSGYDFPWSLTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFT 260

Query: 245 YMDWIFGTDKGFR 253
           Y D+I+GTDKG+R
Sbjct: 261 YCDYIYGTDKGYR 272

BLAST of Carg12392 vs. Swiss-Prot
Match: sp|Q8VWZ8|SMO22_ARATH (Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 1.1e-131
Identity = 214/259 (82.63%), Postives = 241/259 (93.05%), Query Frame = 0

Query: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60
           MA+ +ES W+YL+T+FSDFQLAC+GSF++HESVFFLSGLPFIFLE+ G+LSKYKIQ K N
Sbjct: 1   MASFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLERQGFLSKYKIQTKNN 60

Query: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120
           + AAQGKCI+RLLLYHF VNLP+M+ SYPVF+ MGMRS+ PLPSWK V  QI+FYFI+ED
Sbjct: 61  TPAAQGKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180
           FVFYWGHRILH+KWLY+NVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL
Sbjct: 121 FVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240
           +TLWLWMVLRVLETVEAHCGYHFPWS SNF+PLYGGA FHDYHHRLL+TKSGNYSSTF Y
Sbjct: 181 ITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVY 240

Query: 241 MDWIFGTDKGFRNLEAIKK 260
           MDWIFGTDKG+R L+ +K+
Sbjct: 241 MDWIFGTDKGYRRLKTLKE 259

BLAST of Carg12392 vs. Swiss-Prot
Match: sp|Q9ZW22|SMO21_ARATH (Methylsterol monooxygenase 2-1 OS=Arabidopsis thaliana OX=3702 GN=SMO2-1 PE=2 SV=2)

HSP 1 Score: 459.1 bits (1180), Expect = 3.2e-128
Identity = 212/271 (78.23%), Postives = 241/271 (88.93%), Query Frame = 0

Query: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60
           M +L+ES W+YL+T+FSDFQLAC+GSFI+HESVFFLSGLP+IFLE+ G+LS YKIQ K+N
Sbjct: 1   MDSLVESGWKYLVTHFSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSN 60

Query: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120
           +  AQGKCI+RLLLYH  VNLP+M+ SYPVF+ MGM S+ PLPSWKVV  QI+FYFI+ED
Sbjct: 61  TPEAQGKCIARLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180
           FVFYWGHRILHTKWLY+NVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGG------------AYFHDYHHRLLF 240
           +TLWLWM+LRV+ETVEAHCGYHFPWSPSNF+PLYGG            A FHDYHHRLL+
Sbjct: 181 ITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLY 240

Query: 241 TKSGNYSSTFTYMDWIFGTDKGFRNLEAIKK 260
           TKSGNYSSTF YMDWIFGTDKG+R L+A+K+
Sbjct: 241 TKSGNYSSTFVYMDWIFGTDKGYRKLKALKE 271

BLAST of Carg12392 vs. Swiss-Prot
Match: sp|Q55D54|MSMOB_DICDI (Putative methylsterol monooxygenase DDB_G0269788 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0269788 PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 5.4e-59
Identity = 111/258 (43.02%), Postives = 159/258 (61.63%), Query Frame = 0

Query: 5   LESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQ-AKTNSSA 64
           +E  W   +  + +  L   G+FI HE  +F S +PF   +   +L KYKIQ  K N   
Sbjct: 9   IEPYWFKFVDYYGEDFLITYGTFIAHEVFYFGSFIPFFLCDFMPFLQKYKIQPTKKNEWK 68

Query: 65  AQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVEDFVF 124
            Q  CI ++L+ H  V LP+M +  P  K +G+ +  PLPS   +   I   F++EDF F
Sbjct: 69  TQFNCIFKVLMTHIFVQLPMMYIFDPAIKAIGLSARAPLPSIPYLIFTIACCFLIEDFYF 128

Query: 125 YWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTL 184
           YW HR LH  + Y+++H VHH++A PFG+T+EYAHP E + LG  T++GP L    L TL
Sbjct: 129 YWVHRALHHGFWYKHIHKVHHDHAAPFGMTAEYAHPLETVILGVGTVIGPFLFSRDLFTL 188

Query: 185 WLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTYMDW 244
           W+W+  R+ +TVE H GY FPW+P+  IP +GG++FHD+HH    T  GNYSSTFTY+D 
Sbjct: 189 WVWLGTRLFQTVECHSGYDFPWNPTKLIPFWGGSHFHDFHHE---TFVGNYSSTFTYLDK 248

Query: 245 IFGTDKGFRNLEAIKKAE 262
           IFGT   + + + I+ ++
Sbjct: 249 IFGTSDKYYSRKQIRDSK 263

BLAST of Carg12392 vs. Swiss-Prot
Match: sp|Q8L7W5|SMO11_ARATH (Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana OX=3702 GN=SMO1-1 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 9.2e-59
Identity = 115/265 (43.40%), Postives = 160/265 (60.38%), Query Frame = 0

Query: 5   LESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKA----GWLSKYKIQAKTN 64
           LE+ W       SD+ L C     +   VF L  LP +F+E A    G  ++YKIQ K N
Sbjct: 21  LETLWFDYSATKSDYYLYCHNILFLF-LVFSLVPLPLVFVELARSASGLFNRYKIQPKVN 80

Query: 65  SSAA-QGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVE 124
            S +   KC   ++     V  P+ +VSYP  +++ +RS LPLP+   +  Q++ YF++E
Sbjct: 81  YSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGLPLPTITEMLSQLVVYFLIE 140

Query: 125 DFVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPH 184
           D+  YW HR  H+KW Y  +H VHHEY  P G  + YAH AE+L LG  T +GPA+   H
Sbjct: 141 DYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWAEVLLLGIPTFMGPAIAPGH 200

Query: 185 LMTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFT 244
           ++T WLW+ LR +E +E H GY FPWSP+ +IP YGGA +HDYHH +      N++S FT
Sbjct: 201 MITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFT 260

Query: 245 YMDWIFGTDKGFR----NLEAIKKA 261
           Y D+I+GTDKG+R     LE IK++
Sbjct: 261 YCDYIYGTDKGYRFQKKLLEQIKES 284

BLAST of Carg12392 vs. Swiss-Prot
Match: sp|F4JLZ6|SMO13_ARATH (Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana OX=3702 GN=SMO1-3 PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 2.9e-57
Identity = 106/248 (42.74%), Postives = 150/248 (60.48%), Query Frame = 0

Query: 6   ESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTNSSAA- 65
           E+ W       S+F + C  + ++   VF L+  P + +E  GW  ++KIQ K   S + 
Sbjct: 22  ETVWFDYSATKSNFHVYC-HTILVLFLVFSLAPFPLVIVEWTGWFDQFKIQKKVKYSLSD 81

Query: 66  QGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVEDFVFY 125
             +C   ++     V   + IVSYP  +++G+RS LPLPS   +  Q++ YF++ED+  Y
Sbjct: 82  MFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPLPSLMEIVAQLVVYFLIEDYTNY 141

Query: 126 WGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLMTLW 185
           W HR +H KW Y+ +H +HHEY +P G  S YAH AEIL LG  T +GPA+   H+MT W
Sbjct: 142 WIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEILILGIPTFLGPAIAPGHIMTFW 201

Query: 186 LWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTYMDWI 245
           LW+ LR  E +E H GY FPWS +  IP YGG  +HDYHH +      N++S FTY D+I
Sbjct: 202 LWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDYHHYVGGQSQSNFASVFTYCDYI 261

Query: 246 FGTDKGFR 253
           +GTDKG+R
Sbjct: 262 YGTDKGYR 268

BLAST of Carg12392 vs. TrEMBL
Match: tr|A0A1S3C6T9|A0A1S3C6T9_CUCME (methylsterol monooxygenase 2-2 OS=Cucumis melo OX=3656 GN=LOC103497129 PE=3 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 1.1e-146
Identity = 248/262 (94.66%), Postives = 257/262 (98.09%), Query Frame = 0

Query: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60
           MAALLESAW+YLITNFSDFQLAC+GSFIIHESVFFLSGLPFI LE+AGWLSKYKIQAK N
Sbjct: 1   MAALLESAWQYLITNFSDFQLACIGSFIIHESVFFLSGLPFILLERAGWLSKYKIQAKNN 60

Query: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120
           S AAQGKCISRLLLYHFGVNLPVM+VSYPVFK MGMRSTLPLPSWKVVFGQIIFYFI+ED
Sbjct: 61  SPAAQGKCISRLLLYHFGVNLPVMLVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180
           FVFYWGHRILHTKWLY+NVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240
            TLWLWMV+RVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLL+TKSGNYSSTFTY
Sbjct: 181 FTLWLWMVVRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAES 263
           MDWIFGTDKGFRNLEAIKKAE+
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAEN 262

BLAST of Carg12392 vs. TrEMBL
Match: tr|A0A0A0LVE5|A0A0A0LVE5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G524750 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.6e-145
Identity = 246/262 (93.89%), Postives = 256/262 (97.71%), Query Frame = 0

Query: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60
           MAALLESAW+YLITNFSDFQLAC+GSFIIHESVFFLSGLPFI LE+AGWLSKYKIQAK N
Sbjct: 1   MAALLESAWQYLITNFSDFQLACIGSFIIHESVFFLSGLPFILLERAGWLSKYKIQAKNN 60

Query: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120
           S AAQGKCISRLLLYHFGVNLPVM+VSYPVFK MGMRSTLPLPSWKVVFGQIIFYFI+ED
Sbjct: 61  SPAAQGKCISRLLLYHFGVNLPVMLVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180
           FVFYWGHRILHTKWLY+NVHSVHHEYATPFGLTSEYAHPAEILFLGFATI+GPALTGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIIGPALTGPHL 180

Query: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240
            TLWLWMV+RVLETVEAHCGY FPWSPSNFIPLYGGAYFHDYHHRLL+TKSGNYSSTFTY
Sbjct: 181 FTLWLWMVVRVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAES 263
           MDWIFGTDKGFRNLEAIKKAE+
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAEN 262

BLAST of Carg12392 vs. TrEMBL
Match: tr|V4UFS8|V4UFS8_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10029052mg PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 2.4e-133
Identity = 223/258 (86.43%), Postives = 244/258 (94.57%), Query Frame = 0

Query: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60
           MA+++ES WRYLIT+FSDFQLAC+GSF +HES+FFL+GLPFI+LE+AGWLSKYKIQ K N
Sbjct: 1   MASIIESGWRYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKNN 60

Query: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120
           S AAQ KCI+RLLLYHFGVNLPVMI SYPVFK MGM+S+LPLPSWKVV  QIIFYFI+ED
Sbjct: 61  SPAAQEKCITRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLPLPSWKVVLSQIIFYFILED 120

Query: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180
           FVFYWGHRILHTKWLY++VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180

Query: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240
           MTLWLWMVLRVLETVEAHCGYHFPWS SNFIPLYGGA FHDYHHRLL+TKSGNYSSTF Y
Sbjct: 181 MTLWLWMVLRVLETVEAHCGYHFPWSLSNFIPLYGGADFHDYHHRLLYTKSGNYSSTFVY 240

Query: 241 MDWIFGTDKGFRNLEAIK 259
           MDWIFGTDKG+R L+A+K
Sbjct: 241 MDWIFGTDKGYRKLKALK 258

BLAST of Carg12392 vs. TrEMBL
Match: tr|A0A2H5NCU1|A0A2H5NCU1_CITUN (Uncharacterized protein (Fragment) OS=Citrus unshiu OX=55188 GN=CUMW_033380 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 2.4e-133
Identity = 223/258 (86.43%), Postives = 244/258 (94.57%), Query Frame = 0

Query: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60
           MA+++ES WRYLIT+FSDFQLAC+GSF +HES+FFL+GLPFI+LE+AGWLSKYKIQ K N
Sbjct: 37  MASIIESGWRYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKNN 96

Query: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120
           S AAQ KCI+RLLLYHFGVNLPVMI SYPVFK MGM+S+LPLPSWKVV  QIIFYFI+ED
Sbjct: 97  SPAAQEKCITRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLPLPSWKVVLSQIIFYFILED 156

Query: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180
           FVFYWGHRILHTKWLY++VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHL
Sbjct: 157 FVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 216

Query: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240
           MTLWLWMVLRVLETVEAHCGYHFPWS SNFIPLYGGA FHDYHHRLL+TKSGNYSSTF Y
Sbjct: 217 MTLWLWMVLRVLETVEAHCGYHFPWSLSNFIPLYGGADFHDYHHRLLYTKSGNYSSTFVY 276

Query: 241 MDWIFGTDKGFRNLEAIK 259
           MDWIFGTDKG+R L+A+K
Sbjct: 277 MDWIFGTDKGYRKLKALK 294

BLAST of Carg12392 vs. TrEMBL
Match: tr|A0A067FH15|A0A067FH15_CITSI (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g024309mg PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 2.4e-133
Identity = 223/258 (86.43%), Postives = 244/258 (94.57%), Query Frame = 0

Query: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60
           MA+++ES WRYLIT+FSDFQLAC+GSF +HES+FFL+GLPFI+LE+AGWLSKYKIQ K N
Sbjct: 1   MASIIESGWRYLITHFSDFQLACLGSFFLHESIFFLAGLPFIYLERAGWLSKYKIQTKNN 60

Query: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120
           S AAQ KCI+RLLLYHFGVNLPVMI SYPVFK MGM+S+LPLPSWKVV  QIIFYFI+ED
Sbjct: 61  SPAAQEKCITRLLLYHFGVNLPVMIFSYPVFKYMGMQSSLPLPSWKVVLSQIIFYFILED 120

Query: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180
           FVFYWGHRILHTKWLY++VHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHL 180

Query: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240
           MTLWLWMVLRVLETVEAHCGYHFPWS SNFIPLYGGA FHDYHHRLL+TKSGNYSSTF Y
Sbjct: 181 MTLWLWMVLRVLETVEAHCGYHFPWSLSNFIPLYGGADFHDYHHRLLYTKSGNYSSTFVY 240

Query: 241 MDWIFGTDKGFRNLEAIK 259
           MDWIFGTDKG+R L+A+K
Sbjct: 241 MDWIFGTDKGYRKLKALK 258

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022948631.12.1e-152100.00methylsterol monooxygenase 2-2-like [Cucurbita moschata][more]
XP_022998327.17.9e-15299.62methylsterol monooxygenase 2-2-like [Cucurbita maxima][more]
XP_023521502.18.8e-15199.23methylsterol monooxygenase 2-2-like [Cucurbita pepo subsp. pepo] >XP_023523161.1... [more]
XP_022964698.11.5e-14796.18methylsterol monooxygenase 2-2 [Cucurbita moschata] >XP_022970560.1 methylsterol... [more]
XP_008457442.11.7e-14694.66PREDICTED: methylsterol monooxygenase 2-2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G07420.15.9e-13382.63sterol 4-alpha-methyl-oxidase 2-1[more]
AT2G29390.11.8e-12978.23sterol 4-alpha-methyl-oxidase 2-2[more]
AT4G12110.15.1e-6043.40sterol-4alpha-methyl oxidase 1-1[more]
AT4G22753.11.6e-5842.74sterol 4-alpha methyl oxidase 1-3[more]
AT4G22756.16.2e-5842.69sterol C4-methyl oxidase 1-2[more]
Match NameE-valueIdentityDescription
sp|Q8VWZ8|SMO22_ARATH1.1e-13182.63Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV... [more]
sp|Q9ZW22|SMO21_ARATH3.2e-12878.23Methylsterol monooxygenase 2-1 OS=Arabidopsis thaliana OX=3702 GN=SMO2-1 PE=2 SV... [more]
sp|Q55D54|MSMOB_DICDI5.4e-5943.02Putative methylsterol monooxygenase DDB_G0269788 OS=Dictyostelium discoideum OX=... [more]
sp|Q8L7W5|SMO11_ARATH9.2e-5943.40Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana OX=3702 GN=SMO1-1 PE=1 SV... [more]
sp|F4JLZ6|SMO13_ARATH2.9e-5742.74Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana OX=3702 GN=SMO1-3 PE=2 SV... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3C6T9|A0A1S3C6T9_CUCME1.1e-14694.66methylsterol monooxygenase 2-2 OS=Cucumis melo OX=3656 GN=LOC103497129 PE=3 SV=1[more]
tr|A0A0A0LVE5|A0A0A0LVE5_CUCSA1.6e-14593.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G524750 PE=3 SV=1[more]
tr|V4UFS8|V4UFS8_9ROSI2.4e-13386.43Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10029052mg PE=3 ... [more]
tr|A0A2H5NCU1|A0A2H5NCU1_CITUN2.4e-13386.43Uncharacterized protein (Fragment) OS=Citrus unshiu OX=55188 GN=CUMW_033380 PE=3... [more]
tr|A0A067FH15|A0A067FH15_CITSI2.4e-13386.43Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g024309mg PE=3 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005506iron ion binding
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO:0008610lipid biosynthetic process
Vocabulary: INTERPRO
TermDefinition
IPR006694Fatty_acid_hydroxylase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008610 lipid biosynthetic process
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12392-RACarg12392-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 113..247
e-value: 3.6E-29
score: 101.7
NoneNo IPR availablePANTHERPTHR11863STEROL DESATURASEcoord: 1..261
NoneNo IPR availablePANTHERPTHR11863:SF96METHYLSTEROL MONOOXYGENASE 2-1-RELATEDcoord: 1..261

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg12392Carg20636Silver-seed gourdcarcarB408