Carg12327 (gene) Silver-seed gourd

Overview
NameCarg12327
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionMitogen-activated protein kinase
LocationCucurbita_argyrosperma_scaffold_074 : 335685 .. 337831 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAACGATTGCACGGCTGTGGAGATCAATGGCCTTCCCACCTACGATGGAAGGTACATCCTCTACAACGTTCTCGGCAACTTATTTGAGGTCTCCGCCAAGTACTCCCCTTCAATTCAGCCTGTTGGACGCGGTGCTTACGCCATTGTTTGGTACTTCTTTTCCTCCTCTTTCGTTTTTCGCTTTGCTTCTAAGTTCTTCGATTTTATTTTGGTATATGCTAAGATTTTGATCATCGACGAATTCGGAGACGAAAGATGAGGTTGCGATAAAGAAGATAGGGAATGCTTTTGATAACTGGGTTGATACCAAGAGAACTCTTCGTGAGATTAAACTTCTATGCCATATGGACCATGAAAATGTATTTCGCTCAAATAAACATTCTTCATTTGGGTGCAAATTTTGCTATGCTTTAACATCCATTTATTGCTGTGATTTGTTCTTAGCAAATTCCTCATATCCAGGTTGAACTTCAGCTTAATTAGTGTTTAATTTTGAACTTTTTATTTATATGTTTCTACTTCAGCTCTGTTTTTCTCATGCCATGAACAGATTTAGTTAATGTTTAGTTGCCCTGGGGACTTATTTCATTCTGCCATTGAAGCATAATGATCTGTTTCTAACTTCCTTTCAGATTATAAAGATCAAAGATATAATTCCTCCACCAGAAAAGGAAAAATTCAATGATGTGTATATTGTGTATGTATTAATGGACACTGACCTGCATCAGATCATTTGCTCTAGCCAAGCTCTAACAGACGATCACTGTCAGGTAAGGGACACTTACCATTCCAGTTAGATCTTAGCAAAAGTTTGTTTCAATTCTTAGTACAAAGTTTTTATGTTGATTATGATCTTGTTGGAGGAACAAGAAAGATTTGAAACTGTCTTGAATGATTTTATGTTTGTTGTTTGGACAGTACTTCTTATACCAGTTACTACGAGGTTTGAAGTATTTACACTCTGCAAATGTCTTGCACCGAGATCTAAAACCAGGCAATCTGCTTCTCAATGCAAATTGCGACCTCAAGATTTGTGATTTTGGGTTAGCAAGAACAACATCAGAGACGGATTTTATGACAGAATATGTTGTAACCAGATGGTATCGAGCTCCTGAGCTACTACTTAACACCTCTGAATACACTGCAGCCATAGATATTTGGTCTGTTGGATGTATTCTCATGGAAATTCTAAGGAGGGAGCCACTGTTCCCTGGTAAAGATTATGCGTAACAACTGGGGCTTATAACCGAGGTACTTGATCATTGTCTTCCAATTTTCTGCATGATGTTTGAAATTATGCATTCAAACGCTAAGGTGATGTGAGATCCCACGTCGGTTGGAGAGGAGAACTAAACATTCCTTATAAGGGTGAGGTTAGATGCGTTTTAAAACCTTGAGGCTGACGGCAATACGTAACGAGCCAAAATGGACAATATCGGCTAGTGGTGGGTTTTGGGCTGTTATAAATAGTATCAAAGTTAGACATCGGGCGGTGTACCAGCGAGGATGCTAGGCTCCAAGGGAGGTGGATTGTAAGAGATCCCACCTCGGTTGGAAAAGGGAACGAAACATTTCTTATAAGAATGCGGAAAACCTCTCCCTAGTAGATGCATTTTGAAACCGTGAGGCTGGTAACGGGCAAAAGCAGACAATATCTGTTAGTGGTGGGTTTGAGCGAATACAAATGTTATCAAAGCCAGACACTGGGCGGTGTGCCAGCGAGGACGCTGGTCCCAAGAGGTGAATTGTGAGATCTCACATCAGTTAGAGGGGGGAACAAAACATTCCTTATAAGGGTAGTAGACGCGCTTTAAACCCGTGAGACTGACGGTGATACGTAACGAGATAAAACAGACAATATCTATTAACGGTGGGTTTGAGCTATTACATATAGTCAATTTTTACAGTAGTAGTAGTCAGTTTCGTTAACTGCCTAAAATGTTATTTGCATATGTTTGCTGCTCTTCCTGGTATTATTTTTATGGTTTAATACTAAAACATAGTTTTGTTTCTTAGATTGTGAGTTTATGTTCGAGTATAGTTTGCTTTTCGTGCCATTGGGTTTGGTTTGAAAGATCAAACATTTAATTTTGGTCCTTAAACTTTTAAAATTGACATTAATTTTTATAGCTCAGAAAATAG

mRNA sequence

ATGGAAAACGATTGCACGGCTGTGGAGATCAATGGCCTTCCCACCTACGATGGAAGGTACATCCTCTACAACGTTCTCGGCAACTTATTTGAGGTCTCCGCCAAGTACTCCCCTTCAATTCAGCCTGTTGGACGCGGTGCTTACGCCATTGTTTGGTACTTCTTTTCCTCCTCTTTCGTTTTTCGCTTTGCTTCTAAGTTCTTCGATTTTATTTTGACGAAAGATGAGGTTGCGATAAAGAAGATAGGGAATGCTTTTGATAACTGGGTTGATACCAAGAGAACTCTTCGTGAGATTAAACTTCTATGCCATATGGACCATGAAAATATTATAAAGATCAAAGATATAATTCCTCCACCAGAAAAGGAAAAATTCAATGATGTGTATATTGTGTATGTATTAATGGACACTGACCTGCATCAGATCATTTGCTCTAGCCAAGCTCTAACAGACGATCACTGTCAGCTCAGAAAATAG

Coding sequence (CDS)

ATGGAAAACGATTGCACGGCTGTGGAGATCAATGGCCTTCCCACCTACGATGGAAGGTACATCCTCTACAACGTTCTCGGCAACTTATTTGAGGTCTCCGCCAAGTACTCCCCTTCAATTCAGCCTGTTGGACGCGGTGCTTACGCCATTGTTTGGTACTTCTTTTCCTCCTCTTTCGTTTTTCGCTTTGCTTCTAAGTTCTTCGATTTTATTTTGACGAAAGATGAGGTTGCGATAAAGAAGATAGGGAATGCTTTTGATAACTGGGTTGATACCAAGAGAACTCTTCGTGAGATTAAACTTCTATGCCATATGGACCATGAAAATATTATAAAGATCAAAGATATAATTCCTCCACCAGAAAAGGAAAAATTCAATGATGTGTATATTGTGTATGTATTAATGGACACTGACCTGCATCAGATCATTTGCTCTAGCCAAGCTCTAACAGACGATCACTGTCAGCTCAGAAAATAG

Protein sequence

MENDCTAVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFVFRFASKFFDFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPPEKEKFNDVYIVYVLMDTDLHQIICSSQALTDDHCQLRK
Homology
[Close]
BLAST of Carg12327 vs. NCBI nr
Match: XP_023524579.1 (mitogen-activated protein kinase homolog NTF6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 300.1 bits (767), Expect = 4.6e-78
Identity = 150/173 (86.71%), Postives = 152/173 (87.86%), Query Frame = 0 

Query: 1   MENDCTAVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFV 60
           MEND TAVEINGLPTYDGRYILYNVLGNLFEVSAKY+PSIQPVGRGAYAIVWYFFSSSFV
Sbjct: 1   MENDYTAVEINGLPTYDGRYILYNVLGNLFEVSAKYAPSIQPVGRGAYAIVWYFFSSSFV 60

Query: 61  FRFASKFFDFIL------------------TKDEVAIKKIGNAFDNWVDTKRTLREIKLL 120
           FRFASKFFD+IL                  TKDEVAIKKIGNAFDNWVD KRTLREIKLL
Sbjct: 61  FRFASKFFDYILVYAKILIICLLGCTTNSETKDEVAIKKIGNAFDNWVDAKRTLREIKLL 120

Query: 121 CHMDHENIIKIKDIIPPPEKEKFNDVYIVYVLMDTDLHQIICSSQALTDDHCQ 156
           CHMDHENIIKIKDIIPPPEKEKFNDVYIVY LMDTDLHQIICSSQALTDDHCQ
Sbjct: 121 CHMDHENIIKIKDIIPPPEKEKFNDVYIVYELMDTDLHQIICSSQALTDDHCQ 173

[Close]
BLAST of Carg12327 vs. NCBI nr
Match: XP_022948704.1 (mitogen-activated protein kinase homolog NTF6-like [Cucurbita moschata])

HSP 1 Score: 277.7 bits (709), Expect = 2.4e-71
Identity = 137/155 (88.39%), Postives = 138/155 (89.03%), Query Frame = 0 

Query: 1   MENDCTAVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFV 60
           MENDCTAVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWY  +S   
Sbjct: 1   MENDCTAVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYSTNSE-- 60

Query: 61  FRFASKFFDFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPP 120
                       TKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPP
Sbjct: 61  ------------TKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPP 120

Query: 121 EKEKFNDVYIVYVLMDTDLHQIICSSQALTDDHCQ 156
           EKEKFNDVYIVYVLMDTDLHQIICSSQALTDDHCQ
Sbjct: 121 EKEKFNDVYIVYVLMDTDLHQIICSSQALTDDHCQ 141

[Close]
BLAST of Carg12327 vs. NCBI nr
Match: XP_022964848.1 (mitogen-activated protein kinase homolog NTF6 [Cucurbita moschata] >XP_023519830.1 mitogen-activated protein kinase homolog NTF6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 233.8 bits (595), Expect = 4.0e-58
Identity = 117/155 (75.48%), Postives = 127/155 (81.94%), Query Frame = 0 

Query: 1   MENDCTAVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFV 60
           MEND +A+EI G PTYDG+Y+LYNVLGN FEVSAKY+PSIQPVGRGAY IV    +S   
Sbjct: 1   MENDSSAMEIKGFPTYDGKYLLYNVLGNFFEVSAKYAPSIQPVGRGAYGIVCCTTNSE-- 60

Query: 61  FRFASKFFDFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPP 120
                       TK+EVAIKKIGNAFDN +D KRTLREI+LLCHMDH+NIIKIKDIIPPP
Sbjct: 61  ------------TKEEVAIKKIGNAFDNRIDAKRTLREIELLCHMDHDNIIKIKDIIPPP 120

Query: 121 EKEKFNDVYIVYVLMDTDLHQIICSSQALTDDHCQ 156
           EKEKFNDVYIVY LMDTDLHQII SSQALTDDHCQ
Sbjct: 121 EKEKFNDVYIVYELMDTDLHQIIRSSQALTDDHCQ 141

[Close]
BLAST of Carg12327 vs. NCBI nr
Match: XP_022970376.1 (mitogen-activated protein kinase homolog NTF6 [Cucurbita maxima])

HSP 1 Score: 233.8 bits (595), Expect = 4.0e-58
Identity = 117/155 (75.48%), Postives = 127/155 (81.94%), Query Frame = 0 

Query: 1   MENDCTAVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFV 60
           MEND +A+EI G PTYDG+Y+LYNVLGN FEVSAKY+PSIQPVGRGAY IV    +S   
Sbjct: 1   MENDSSAMEIKGFPTYDGKYLLYNVLGNFFEVSAKYAPSIQPVGRGAYGIVCCTTNSE-- 60

Query: 61  FRFASKFFDFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPP 120
                       TK+EVAIKKIGNAFDN +D KRTLREI+LLCHMDH+NIIKIKDIIPPP
Sbjct: 61  ------------TKEEVAIKKIGNAFDNRIDAKRTLREIELLCHMDHDNIIKIKDIIPPP 120

Query: 121 EKEKFNDVYIVYVLMDTDLHQIICSSQALTDDHCQ 156
           EKEKFNDVYIVY LMDTDLHQII SSQALTDDHCQ
Sbjct: 121 EKEKFNDVYIVYELMDTDLHQIIRSSQALTDDHCQ 141

[Close]
BLAST of Carg12327 vs. NCBI nr
Match: XP_004148790.2 (PREDICTED: mitogen-activated protein kinase homolog NTF6 [Cucumis sativus] >KGN64720.1 hypothetical protein Csa_1G077220 [Cucumis sativus])

HSP 1 Score: 224.9 bits (572), Expect = 1.9e-55
Identity = 115/156 (73.72%), Postives = 126/156 (80.77%), Query Frame = 0 

Query: 1   MEND-CTAVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSF 60
           MEND  +A++I G PTYD +Y+LYNVLG+ FEVSAKYSPSIQPVGRGAY IV    +S  
Sbjct: 1   MENDSSSAMDIKGTPTYDSKYLLYNVLGSFFEVSAKYSPSIQPVGRGAYGIVCCTTNSE- 60

Query: 61  VFRFASKFFDFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPP 120
                        TK+EVAIKKIGNAFDN +D KRTLREIKLLCHMDH+NIIKIKDIIPP
Sbjct: 61  -------------TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPP 120

Query: 121 PEKEKFNDVYIVYVLMDTDLHQIICSSQALTDDHCQ 156
           P+KEKFNDVYIVY LMDTDLHQII SSQALTDDHCQ
Sbjct: 121 PDKEKFNDVYIVYELMDTDLHQIIRSSQALTDDHCQ 142

[Close]
BLAST of Carg12327 vs. TAIR10
Match: AT1G07880.2 (Protein kinase superfamily protein)

HSP 1 Score: 191.8 bits (486), Expect = 3.2e-49
Identity = 97/147 (65.99%), Postives = 111/147 (75.51%), Query Frame = 0 

Query: 9   EINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFVFRFASKFF 68
           E  G+ TYDGRY++YNVLGN+FE+S+KY P I+P+GRGAY IV    +S           
Sbjct: 5   EDGGILTYDGRYVMYNVLGNIFELSSKYIPPIEPIGRGAYGIVCCATNSE---------- 64

Query: 69  DFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPPEKEKFNDV 128
               T +EVAIKKI NAFDN VD KRTLREIKLL HMDH+N+IKIKDII  PEKE+F DV
Sbjct: 65  ----TNEEVAIKKIANAFDNRVDAKRTLREIKLLSHMDHDNVIKIKDIIELPEKERFEDV 124

Query: 129 YIVYVLMDTDLHQIICSSQALTDDHCQ 156
           YIVY LMDTDLHQII S+Q LTDDHCQ
Sbjct: 125 YIVYELMDTDLHQIIRSTQTLTDDHCQ 137

[Close]
BLAST of Carg12327 vs. TAIR10
Match: AT4G01370.1 (MAP kinase 4)

HSP 1 Score: 181.0 bits (458), Expect = 5.6e-46
Identity = 91/144 (63.19%), Postives = 106/144 (73.61%), Query Frame = 0 

Query: 12  GLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFVFRFASKFFDFI 71
           G+ T+ G Y+ YNV GNLFEVS KY P ++P+GRGAY IV    +S              
Sbjct: 18  GVATHGGSYVQYNVYGNLFEVSRKYVPPLRPIGRGAYGIVCAATNSE------------- 77

Query: 72  LTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPPEKEKFNDVYIV 131
            T +EVAIKKIGNAFDN +D KRTLREIKLL HMDHEN+I +KDII PP++E FNDVYIV
Sbjct: 78  -TGEEVAIKKIGNAFDNIIDAKRTLREIKLLKHMDHENVIAVKDIIKPPQRENFNDVYIV 137

Query: 132 YVLMDTDLHQIICSSQALTDDHCQ 156
           Y LMDTDLHQII S+Q LTDDHC+
Sbjct: 138 YELMDTDLHQIIRSNQPLTDDHCR 147

[Close]
BLAST of Carg12327 vs. TAIR10
Match: AT4G11330.1 (MAP kinase 5)

HSP 1 Score: 171.0 bits (432), Expect = 5.8e-43
Identity = 89/152 (58.55%), Postives = 103/152 (67.76%), Query Frame = 0 

Query: 4   DCTAVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFVFRF 63
           D     I G+  + GRY  YNV GNLFEVS KY P I+P+GRGAY  V     S      
Sbjct: 10  DLGDTNIKGVLVHGGRYFQYNVYGNLFEVSNKYVPPIRPIGRGAYGFVCAAVDSE----- 69

Query: 64  ASKFFDFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPPEKE 123
                    T +E+AIKKIG AFDN VD KRTLREIKLL H++HEN++ IKDII PP+KE
Sbjct: 70  ---------THEEIAIKKIGKAFDNKVDAKRTLREIKLLRHLEHENVVVIKDIIRPPKKE 129

Query: 124 KFNDVYIVYVLMDTDLHQIICSSQALTDDHCQ 156
            F DVYIV+ LMDTDLHQII S+Q+L DDHCQ
Sbjct: 130 DFVDVYIVFELMDTDLHQIIRSNQSLNDDHCQ 147

[Close]
BLAST of Carg12327 vs. TAIR10
Match: AT2G43790.1 (MAP kinase 6)

HSP 1 Score: 170.6 bits (431), Expect = 7.6e-43
Identity = 84/141 (59.57%), Postives = 102/141 (72.34%), Query Frame = 0 

Query: 15  TYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFVFRFASKFFDFILTK 74
           ++ GR+I YN+ GN+FEV+AKY P I P+G+GAY IV    +S               T 
Sbjct: 41  SHGGRFIQYNIFGNIFEVTAKYKPPIMPIGKGAYGIVCSAMNSE--------------TN 100

Query: 75  DEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPPEKEKFNDVYIVYVL 134
           + VAIKKI NAFDN +D KRTLREIKLL HMDHENI+ I+DIIPPP +  FNDVYI Y L
Sbjct: 101 ESVAIKKIANAFDNKIDAKRTLREIKLLRHMDHENIVAIRDIIPPPLRNAFNDVYIAYEL 160

Query: 135 MDTDLHQIICSSQALTDDHCQ 156
           MDTDLHQII S+QAL+++HCQ
Sbjct: 161 MDTDLHQIIRSNQALSEEHCQ 167

[Close]
BLAST of Carg12327 vs. TAIR10
Match: AT2G46070.1 (mitogen-activated protein kinase 12)

HSP 1 Score: 165.2 bits (417), Expect = 3.2e-41
Identity = 85/146 (58.22%), Postives = 104/146 (71.23%), Query Frame = 0 

Query: 10  INGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFVFRFASKFFD 69
           I  +PT+ GRY+ YNV G LFEVS KY P I+P+GRGA  IV    +S            
Sbjct: 14  IKVVPTHGGRYVQYNVYGQLFEVSRKYVPPIRPIGRGACGIVCAAVNS------------ 73

Query: 70  FILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPPEKEKFNDVY 129
             +T ++VAIKKIGNAFDN +D KRTLREIKLL HMDHEN+I IKDI+ PP+++ FNDVY
Sbjct: 74  --VTGEKVAIKKIGNAFDNIIDAKRTLREIKLLRHMDHENVITIKDIVRPPQRDIFNDVY 133

Query: 130 IVYVLMDTDLHQIICSSQALTDDHCQ 156
           IVY LMDTDL +I+ S+Q LT D C+
Sbjct: 134 IVYELMDTDLQRILRSNQTLTSDQCR 145

[Close]
BLAST of Carg12327 vs. Swiss-Prot
Match: sp|Q40531|NTF6_TOBAC (Mitogen-activated protein kinase homolog NTF6 OS=Nicotiana tabacum OX=4097 GN=NTF6 PE=2 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 1.9e-51
Identity = 103/156 (66.03%), Postives = 121/156 (77.56%), Query Frame = 0 

Query: 1   MENDCT-AVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSF 60
           MEN+    +EI G+PT++G+Y+ YNVLGN FEV++KY P IQPVGRGAY +V    +S  
Sbjct: 1   MENETNEKLEIKGIPTHEGKYVEYNVLGNFFEVTSKYIPPIQPVGRGAYGMVCCATNSE- 60

Query: 61  VFRFASKFFDFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPP 120
                        TK+EVAIKKIGNAF+N +D KRTLREIKLL HMDHENIIKIKDI+ P
Sbjct: 61  -------------TKEEVAIKKIGNAFENRIDAKRTLREIKLLSHMDHENIIKIKDIVRP 120

Query: 121 PEKEKFNDVYIVYVLMDTDLHQIICSSQALTDDHCQ 156
           P++E+FNDVYIVY LMDTDLHQII SSQALTDDHCQ
Sbjct: 121 PDREEFNDVYIVYELMDTDLHQIIRSSQALTDDHCQ 142

[Close]
BLAST of Carg12327 vs. Swiss-Prot
Match: sp|Q9LQQ9|MPK13_ARATH (Mitogen-activated protein kinase 13 OS=Arabidopsis thaliana OX=3702 GN=MPK13 PE=1 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 5.7e-48
Identity = 97/147 (65.99%), Postives = 111/147 (75.51%), Query Frame = 0 

Query: 9   EINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFVFRFASKFF 68
           E  G+ TYDGRY++YNVLGN+FE+S+KY P I+P+GRGAY IV    +S           
Sbjct: 5   EDGGILTYDGRYVMYNVLGNIFELSSKYIPPIEPIGRGAYGIVCCATNSE---------- 64

Query: 69  DFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPPEKEKFNDV 128
               T +EVAIKKI NAFDN VD KRTLREIKLL HMDH+N+IKIKDII  PEKE+F DV
Sbjct: 65  ----TNEEVAIKKIANAFDNRVDAKRTLREIKLLSHMDHDNVIKIKDIIELPEKERFEDV 124

Query: 129 YIVYVLMDTDLHQIICSSQALTDDHCQ 156
           YIVY LMDTDLHQII S+Q LTDDHCQ
Sbjct: 125 YIVYELMDTDLHQIIRSTQTLTDDHCQ 137

[Close]
BLAST of Carg12327 vs. Swiss-Prot
Match: sp|Q336X9|MPK6_ORYSJ (Mitogen-activated protein kinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=MPK6 PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 9.1e-46
Identity = 94/147 (63.95%), Postives = 108/147 (73.47%), Query Frame = 0 

Query: 9   EINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFVFRFASKFF 68
           +I G+ T+ GRY+LYNV GN FEVS+KY+P I+P+GRGAY IV    +S           
Sbjct: 15  QIKGMGTHGGRYVLYNVYGNFFEVSSKYAPPIRPIGRGAYGIVCAAVNSE---------- 74

Query: 69  DFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPPEKEKFNDV 128
                 +EVAIKKIGNAFDN +D KRTLREIKLL HMDHENII IKDII PP ++ FNDV
Sbjct: 75  ----NGEEVAIKKIGNAFDNHIDAKRTLREIKLLRHMDHENIIAIKDIIRPPRRDNFNDV 134

Query: 129 YIVYVLMDTDLHQIICSSQALTDDHCQ 156
           YIV  LMDTDLHQII S+Q LTDDHCQ
Sbjct: 135 YIVSELMDTDLHQIIRSNQPLTDDHCQ 147

[Close]
BLAST of Carg12327 vs. Swiss-Prot
Match: sp|A9S9Q8|MPK4B_PHYPA (Mitogen-activated protein kinase 4b OS=Physcomitrella patens subsp. patens OX=3218 GN=MPK4b PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 1.6e-45
Identity = 93/146 (63.70%), Postives = 107/146 (73.29%), Query Frame = 0 

Query: 10  INGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFVFRFASKFFD 69
           I G+PT++G Y  YN+ GNLFEVS KY P I+P+GRGAY IV    +S            
Sbjct: 16  IQGVPTHNGEYTQYNIFGNLFEVSRKYVPPIRPIGRGAYGIVCSAVNSE----------- 75

Query: 70  FILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPPEKEKFNDVY 129
              T +EVAIKKIGNAFDN +D KRTLREIKLL HMDHENI+ I+DII PP +E FNDVY
Sbjct: 76  ---TGEEVAIKKIGNAFDNRIDAKRTLREIKLLRHMDHENIVAIRDIIRPPTRENFNDVY 135

Query: 130 IVYVLMDTDLHQIICSSQALTDDHCQ 156
           IVY LMDTDLHQII S+Q LT+DHCQ
Sbjct: 136 IVYELMDTDLHQIIRSNQPLTEDHCQ 147

[Close]
BLAST of Carg12327 vs. Swiss-Prot
Match: sp|Q39024|MPK4_ARATH (Mitogen-activated protein kinase 4 OS=Arabidopsis thaliana OX=3702 GN=MPK4 PE=1 SV=2)

HSP 1 Score: 181.0 bits (458), Expect = 1.0e-44
Identity = 91/144 (63.19%), Postives = 106/144 (73.61%), Query Frame = 0 

Query: 12  GLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFVFRFASKFFDFI 71
           G+ T+ G Y+ YNV GNLFEVS KY P ++P+GRGAY IV    +S              
Sbjct: 18  GVATHGGSYVQYNVYGNLFEVSRKYVPPLRPIGRGAYGIVCAATNSE------------- 77

Query: 72  LTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPPEKEKFNDVYIV 131
            T +EVAIKKIGNAFDN +D KRTLREIKLL HMDHEN+I +KDII PP++E FNDVYIV
Sbjct: 78  -TGEEVAIKKIGNAFDNIIDAKRTLREIKLLKHMDHENVIAVKDIIKPPQRENFNDVYIV 137

Query: 132 YVLMDTDLHQIICSSQALTDDHCQ 156
           Y LMDTDLHQII S+Q LTDDHC+
Sbjct: 138 YELMDTDLHQIIRSNQPLTDDHCR 147

[Close]
BLAST of Carg12327 vs. TrEMBL
Match: tr|A0A0A0LS13|A0A0A0LS13_CUCSA (Mitogen-activated protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1G077220 PE=3 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-55
Identity = 115/156 (73.72%), Postives = 126/156 (80.77%), Query Frame = 0 

Query: 1   MEND-CTAVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSF 60
           MEND  +A++I G PTYD +Y+LYNVLG+ FEVSAKYSPSIQPVGRGAY IV    +S  
Sbjct: 1   MENDSSSAMDIKGTPTYDSKYLLYNVLGSFFEVSAKYSPSIQPVGRGAYGIVCCTTNSE- 60

Query: 61  VFRFASKFFDFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPP 120
                        TK+EVAIKKIGNAFDN +D KRTLREIKLLCHMDH+NIIKIKDIIPP
Sbjct: 61  -------------TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPP 120

Query: 121 PEKEKFNDVYIVYVLMDTDLHQIICSSQALTDDHCQ 156
           P+KEKFNDVYIVY LMDTDLHQII SSQALTDDHCQ
Sbjct: 121 PDKEKFNDVYIVYELMDTDLHQIIRSSQALTDDHCQ 142

[Close]
BLAST of Carg12327 vs. TrEMBL
Match: tr|A0A1S3CNX4|A0A1S3CNX4_CUCME (Mitogen-activated protein kinase OS=Cucumis melo OX=3656 GN=LOC103502937 PE=3 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 6.1e-55
Identity = 114/156 (73.08%), Postives = 125/156 (80.13%), Query Frame = 0 

Query: 1   MEND-CTAVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSF 60
           MEND  +A++I G PTYD +Y+LYNVLG+ FEVSAKYSPSIQPVGRGAY IV    +S  
Sbjct: 1   MENDSSSAMDIKGTPTYDSKYLLYNVLGSFFEVSAKYSPSIQPVGRGAYGIVCCTTNSE- 60

Query: 61  VFRFASKFFDFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPP 120
                        TK+EVAIKKIGNAFDN +D KRTLREIKLLCHMDH+NIIKIKDIIPP
Sbjct: 61  -------------TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPP 120

Query: 121 PEKEKFNDVYIVYVLMDTDLHQIICSSQALTDDHCQ 156
           P+KEKFNDVYIVY LMDTDLHQII SSQ LTDDHCQ
Sbjct: 121 PDKEKFNDVYIVYELMDTDLHQIIRSSQDLTDDHCQ 142

[Close]
BLAST of Carg12327 vs. TrEMBL
Match: tr|A0A2D0ULS2|A0A2D0ULS2_CUCSA (Mitogen-activated protein kinase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 8.0e-55
Identity = 114/156 (73.08%), Postives = 125/156 (80.13%), Query Frame = 0 

Query: 1   MEND-CTAVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSF 60
           MEND  +A++I G PTYD +Y+LYNVLG+ FEVSAKYSPSIQPVGRGAY IV    +S  
Sbjct: 1   MENDSSSAMDIKGTPTYDSKYLLYNVLGSFFEVSAKYSPSIQPVGRGAYGIVCCTTNSE- 60

Query: 61  VFRFASKFFDFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPP 120
                        TK+EVAIKKIGNAFDN +D KRTLREIK LCHMDH+NIIKIKDIIPP
Sbjct: 61  -------------TKEEVAIKKIGNAFDNRIDAKRTLREIKPLCHMDHDNIIKIKDIIPP 120

Query: 121 PEKEKFNDVYIVYVLMDTDLHQIICSSQALTDDHCQ 156
           P+KEKFNDVYIVY LMDTDLHQII SSQALTDDHCQ
Sbjct: 121 PDKEKFNDVYIVYELMDTDLHQIIRSSQALTDDHCQ 142

[Close]
BLAST of Carg12327 vs. TrEMBL
Match: tr|A0A0A7R545|A0A0A7R545_GOSHI (Mitogen-activated protein kinase OS=Gossypium hirsutum OX=3635 GN=MPK12 PE=2 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.3e-54
Identity = 110/155 (70.97%), Postives = 122/155 (78.71%), Query Frame = 0 

Query: 1   MENDCTAVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFV 60
           MEN+ TA+++ G PTYDGRY+ YN+LGN+FEVS+KY P IQPVGRGAY IV    +S   
Sbjct: 1   MENEGTAIDLRGTPTYDGRYVRYNILGNIFEVSSKYVPPIQPVGRGAYGIVCCATNSE-- 60

Query: 61  FRFASKFFDFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPP 120
                       TK+EVAIKKI NAFDN +D KRTLREIKLLCHMDH+NIIKIKDII PP
Sbjct: 61  ------------TKEEVAIKKIANAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIIPP 120

Query: 121 EKEKFNDVYIVYVLMDTDLHQIICSSQALTDDHCQ 156
           EKEKFNDVYI Y LMDTDLHQII SSQALTDDHCQ
Sbjct: 121 EKEKFNDVYIAYELMDTDLHQIIRSSQALTDDHCQ 141

[Close]
BLAST of Carg12327 vs. TrEMBL
Match: tr|A0A2P5QSB5|A0A2P5QSB5_GOSBA (Mitogen-activated protein kinase OS=Gossypium barbadense OX=3634 GN=GOBAR_AA39070 PE=3 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.3e-54
Identity = 110/155 (70.97%), Postives = 122/155 (78.71%), Query Frame = 0 

Query: 1   MENDCTAVEINGLPTYDGRYILYNVLGNLFEVSAKYSPSIQPVGRGAYAIVWYFFSSSFV 60
           MEN+ TA+++ G PTYDGRY+ YN+LGN+FEVS+KY P IQPVGRGAY IV    +S   
Sbjct: 1   MENEGTAIDLRGTPTYDGRYVRYNILGNIFEVSSKYVPPIQPVGRGAYGIVCCATNSE-- 60

Query: 61  FRFASKFFDFILTKDEVAIKKIGNAFDNWVDTKRTLREIKLLCHMDHENIIKIKDIIPPP 120
                       TK+EVAIKKI NAFDN +D KRTLREIKLLCHMDH+NIIKIKDII PP
Sbjct: 61  ------------TKEEVAIKKIANAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIIPP 120

Query: 121 EKEKFNDVYIVYVLMDTDLHQIICSSQALTDDHCQ 156
           EKEKFNDVYI Y LMDTDLHQII SSQALTDDHCQ
Sbjct: 121 EKEKFNDVYIAYELMDTDLHQIIRSSQALTDDHCQ 141

The following BLAST results are available for this feature:
BLAST of Carg12327 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023524579.14.6e-7886.71mitogen-activated protein kinase homolog NTF6-like [Cucurbita pepo subsp. pepo][more]
XP_022948704.12.4e-7188.39mitogen-activated protein kinase homolog NTF6-like [Cucurbita moschata][more]
XP_022964848.14.0e-5875.48mitogen-activated protein kinase homolog NTF6 [Cucurbita moschata] >XP_023519830... [more]
XP_022970376.14.0e-5875.48mitogen-activated protein kinase homolog NTF6 [Cucurbita maxima][more]
XP_004148790.21.9e-5573.72PREDICTED: mitogen-activated protein kinase homolog NTF6 [Cucumis sativus] >KGN6... [more]
BLAST of Carg12327 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G07880.23.2e-4965.99Protein kinase superfamily protein[more]
AT4G01370.15.6e-4663.19MAP kinase 4[more]
AT4G11330.15.8e-4358.55MAP kinase 5[more]
AT2G43790.17.6e-4359.57MAP kinase 6[more]
AT2G46070.13.2e-4158.22mitogen-activated protein kinase 12[more]
BLAST of Carg12327 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q40531|NTF6_TOBAC1.9e-5166.03Mitogen-activated protein kinase homolog NTF6 OS=Nicotiana tabacum OX=4097 GN=NT... [more]
sp|Q9LQQ9|MPK13_ARATH5.7e-4865.99Mitogen-activated protein kinase 13 OS=Arabidopsis thaliana OX=3702 GN=MPK13 PE=... [more]
sp|Q336X9|MPK6_ORYSJ9.1e-4663.95Mitogen-activated protein kinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
sp|A9S9Q8|MPK4B_PHYPA1.6e-4563.70Mitogen-activated protein kinase 4b OS=Physcomitrella patens subsp. patens OX=32... [more]
sp|Q39024|MPK4_ARATH1.0e-4463.19Mitogen-activated protein kinase 4 OS=Arabidopsis thaliana OX=3702 GN=MPK4 PE=1 ... [more]
BLAST of Carg12327 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0LS13|A0A0A0LS13_CUCSA1.2e-5573.72Mitogen-activated protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1G077220 PE=3... [more]
tr|A0A1S3CNX4|A0A1S3CNX4_CUCME6.1e-5573.08Mitogen-activated protein kinase OS=Cucumis melo OX=3656 GN=LOC103502937 PE=3 SV... [more]
tr|A0A2D0ULS2|A0A2D0ULS2_CUCSA8.0e-5573.08Mitogen-activated protein kinase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
tr|A0A0A7R545|A0A0A7R545_GOSHI2.3e-5470.97Mitogen-activated protein kinase OS=Gossypium hirsutum OX=3635 GN=MPK12 PE=2 SV=... [more]
tr|A0A2P5QSB5|A0A2P5QSB5_GOSBA2.3e-5470.97Mitogen-activated protein kinase OS=Gossypium barbadense OX=3634 GN=GOBAR_AA3907... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004672protein kinase activity
GO:0005524ATP binding
Vocabulary: Biological Process
TermDefinition
GO:0006468protein phosphorylation
Vocabulary: INTERPRO
TermDefinition
IPR011009Kinase-like_dom_sf
IPR000719Prot_kinase_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12327-RACarg12327-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 18..134
e-value: 5.4E-51
score: 175.2
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 135..156
e-value: 5.4E-51
score: 175.2
NoneNo IPR availablePANTHERPTHR24055:SF185MITOGEN-ACTIVATED PROTEIN KINASE 13coord: 14..155
NoneNo IPR availablePANTHERPTHR24055MITOGEN-ACTIVATED PROTEIN KINASEcoord: 14..155
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 73..154
e-value: 3.1E-8
score: 33.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 37..158
score: 10.521
IPR011009Protein kinase-like domain superfamilySUPERFAMILYSSF56112Protein kinase-like (PK-like)coord: 40..155

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg12327Cla97C03G053080Watermelon (97103) v2carwmbB0467
Carg12327Bhi08G000301Wax gourdcarwgoB0537
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg12327Carg21041Silver-seed gourdcarcarB447
The following block(s) are covering this gene:
GeneOrganismBlock
Carg12327Silver-seed gourdcarcarB332
Carg12327Silver-seed gourdcarcarB333
Carg12327Silver-seed gourdcarcarB353
Carg12327Silver-seed gourdcarcarB354
Carg12327Cucumber (Gy14) v2carcgybB0449
Carg12327Cucurbita maxima (Rimu)carcmaB0613
Carg12327Cucurbita maxima (Rimu)carcmaB0610
Carg12327Cucurbita maxima (Rimu)carcmaB0614
Carg12327Cucurbita maxima (Rimu)carcmaB0615
Carg12327Cucurbita maxima (Rimu)carcmaB0616
Carg12327Cucurbita moschata (Rifu)carcmoB0594
Carg12327Cucurbita moschata (Rifu)carcmoB0596
Carg12327Cucurbita moschata (Rifu)carcmoB0597
Carg12327Cucurbita moschata (Rifu)carcmoB0598
Carg12327Cucurbita moschata (Rifu)carcmoB0599
Carg12327Cucurbita pepo (Zucchini)carcpeB0617
Carg12327Cucurbita pepo (Zucchini)carcpeB0618
Carg12327Cucurbita pepo (Zucchini)carcpeB0620
Carg12327Cucurbita pepo (Zucchini)carcpeB0622
Carg12327Cucurbita pepo (Zucchini)carcpeB0624
Carg12327Cucurbita pepo (Zucchini)carcpeB0625
Carg12327Wild cucumber (PI 183967)carcpiB0477
Carg12327Wild cucumber (PI 183967)carcpiB0480
Carg12327Cucumber (Chinese Long) v2carcuB0472
Carg12327Cucumber (Chinese Long) v3carcucB0474
Carg12327Bottle gourd (USVL1VR-Ls)carlsiB400
Carg12327Bottle gourd (USVL1VR-Ls)carlsiB401
Carg12327Bottle gourd (USVL1VR-Ls)carlsiB403
Carg12327Melon (DHL92) v3.5.1carmeB0447
Carg12327Melon (DHL92) v3.5.1carmeB0448
Carg12327Melon (DHL92) v3.5.1carmeB0449
Carg12327Melon (DHL92) v3.6.1carmedB0450
Carg12327Melon (DHL92) v3.6.1carmedB0451
Carg12327Watermelon (Charleston Gray)carwcgB0433
Carg12327Watermelon (Charleston Gray)carwcgB0435
Carg12327Watermelon (Charleston Gray)carwcgB0436
Carg12327Watermelon (97103) v1carwmB0440
Carg12327Watermelon (97103) v1carwmB0442
Carg12327Watermelon (97103) v1carwmB0444
Carg12327Watermelon (97103) v2carwmbB0464
Carg12327Wax gourdcarwgoB0530
Carg12327Wax gourdcarwgoB0533