Carg12273 (gene) Silver-seed gourd

NameCarg12273
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionpollen-specific protein SF21
LocationCucurbita_argyrosperma_scaffold_054 : 778957 .. 783631 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGAAAGAGATTGTTAAAACAGACGGAGAGAAAAGAAAGAATCGCTTTGTATATAAGCCAACGAATCCAAAGCCTATGATTCTGGAAGAAGAGAGACGAGAGTAAAAGCGAGCGAGACAGAGACACAGGAGGACCTCCGCGACCGACGGCGACCGGCTGTTTTGGTGGTAGCGCTATCGGACGGACACAGAGCTTCGGCCATGGCAGATTCTAACGACTCTGTTTCCGTCGACATGGAGAAGATCTATCTCGGTGGAACGGTTTGTCTTAATTTTATTCGTTTCTTGCTTTTTTCCACCAGGGTTTGCCTTTAATGGCTTGGTTTATTGTGCAGTTGAATCGTTTTTTATTTCTCGGTTCTTTCTTTTTTGTCGATCGAGAGATTTTAGCGGGGTTTTTTCTTCTCTTCATGATTTGATGGCTTAAAATCCCGTTTTCTTTTTGCTGTGGGGCTGGGGATTCTTATTATGGGTGAAGTGTCTTATGGCATTCTATAGCTTTGTTCGGATGTGAATATAAAGGAGAAAATTATTTGGGGATTATTACTTTCCCACTTTTCTTTTTTTCCCGTTCCGATCTGGAGTTCATTTCCATGGCTCGTTTGACCGAGGATCTTCCATTCATTAGGAAGCTATTAACAATTAAGTCCAACCCTTCTTCCTTAAATGAATGAACTAATTAATTAAGCTAGGCAATGGGTTAGGTTTTGAGGCTACTTTCTTGATTCAGGGATCGGTGTTTTTTCCTACTTGTTTAAATGCTTGATGTGATAACGATGGGATTTGATCATTTCCTATTTCTAGCTTCTGGGGTCCTTTTTGTTTCCTTCCAATTTTTTCCTTCTCTTTTTGGTTTGCATTATGTTCTATGTCGTTGTTATCTAGGCCTTGACATTTTTAAACCCTGTTATTCCTCCTATTCAATTTTCTTTTACCTCTTTTGACGAAGGGTTTTCACCTGGTCAAGCTTTTATGAGGATTAAGACATCTTGTATCTCTTTAATTTGTGTTTTTTTTCTAGGATTTTTAGTTTTTCAGTTGGGGTTTTGGGAATTGTTTGTTTGAACTTCGCAATTATCAATGAGTTTGAAGATGGTAGTGCTTTTGTTAGATATTTTTCCATTTGCTTTCTTGTAGTATTGGTTGCTTATCTTAGTACTTAACTTATCCCTTGAGGATATGATCGTTAATCGCTGGAAGCCCATTACTGAATGAACCAACGCCTTACCCACTTTTTTGCTTCTTATTCTTCTTTTTGTAATGAAACTTTATTTGTGTTGACAGATATGATTGCTTATTTATCTTGGCTGTTGTCATAATTTTTGAGCTAATGATAATTCGAACGCAGAAATATACATTCGTATTTTCGCTCTACTTTTATAAAGTTGTACTAGATTGAAGGAACAAGTTAGCTTGGGCATCTTGAAAGGGAATGCACATGGGCTGTTCTTTTAGGATATAATAGGCTGGAAATGTTGGACTATTTGAACTTTTTTCCTTCCAAAAGGGCTTCCTTTTTGCTCTCCCATAGCTCGTACAGGAATAATATGTGATTGGGAGAGACAACTACAAGATGTTTCTTATTTATATTCTTTTTGATTTGAGTGTTTTGATTCCCCCCAATACTTTTCATATCAAATGCCACTTATGTCATTCACAGGAGCATCATATACGAACTGGCCAGGGTTCTGTGTCTGTTATAGTTTGCGGAGACCAAGACAAGCCGCCACTGATTACCTATCCTGATTTGGCTCTAAATCGTAAGTGCTTGGAGTTATCCGTTCTTCTTAGTCGCCATCTAAGATGCATTAATATGGATACTAGACACAAATACTGTGACAGGTTAGTTTGTAAGTATCTAGGGCATGAACATGGCAAAGACACGTTTACTTAAATATACATTTAACATTTTTACACCAGAAGGAAATTCAAAGTCCATATGTTTATGTATTTATATGCTTAAAAATATGAATTTGATGTGTTTTGCACTAAAAAATCATCATTATATGTCTATTTAGTATACTTAACAAATGTTCGAACTAGATTGACTATGTACAGCTAGTATGTAACTCATTTATTGTGCTAACAAGTGTTTGAGATGTGTCCAACAAGTGTAGGACTTGGACAAGTGAGCTAAACTAAAGTGTCTGTGTTTCTTACATCATCTTCAAAATATTTGATATAACTTTTAAGTGGTTTCTTTTCACTGAGTTTTGCAGATATGTCGTGTTTTCAAGGATTATTCTTCTTCCCTGAAGCAGCTTCTTTGCTACTTCACAACTTTTGCATCTACCATATCAGTCCACCAGGACATGAGGTCTGTATTATTGTTGAGTAAATTTGTGTACGAGGATAACTGTATCTGCTTCTGAATACTATATTCACATAGCTTCCTTTTCGGAACTTTACCAAGAGTTCTTGCAGTTAGGAGCTGCTGAGATTTGTCAGGATAATCCTATGCCCTCTGCTGATGATTTAGCAGATCAAATCCTTGAGGTTCTCAACTATTTTGGGTAATTACAATGCATCCTCTCTCGACTCCTCTTTAGAAGTTTGATATCAAAGCTTGTATAGTTGATTTTGATTTGGCTATTTCTATTACAGACTTGGTGCAGTGATGTGCATGGGGGTAACAGCAGGCGCTTACATTCTTACGCTTTTTGCTGTACGTTCATCTCATTGTGAAATTTATTGTGAAGCTCAATCAAAAGTTTTTTTTGGTCTGACCAATTTATTATTTTACAGTTGAAATATAGAGAACGTGTTCTTGGTTTAATTCTTATATCCCCCTTATGCAAGGCACCGTCTTGGTCCGAGTGGTTCTATAATAAGGTAACCTTTTCAAGCCTTGTTATTCTTCATAGATAGAATTACATTCAAGCTTTGATTCATGTTAAATATCTTGTATCCGTTGTACTTGCAGGTAATGTCTAACTTGTTATACTTCTATGGCATGTGCGGGTTGTTGAAAGAGTGCTTGCTTCAGCGGTACTTCAGCAAGGTTATATGATTTCCATTCTTAGAGTTAATTCACTTTTCTGTGTTCACCTTTGATATTGTTGTGGGTGACTGGATTCATGAGATTGTGTTGTAACTTGCAACAGGAGGTCCGTGGTAGTGCCGAAGTTGCAGAGTCCGATATAGTTCAAGCTTGTAGAAAAGTATGTGCTAGTTTGTCTGTCGTGTATCCTTTTCCGTTTCTCGAACTTTTCTCGTTGTTGGTTCAAATTTTGGGTTGGTTTGATAAAGATGGTATATCTGTTCTGTCAGTTGTTAGATGAGAGACAGAGCAACAATGTTTTGAGGTTCCTCCAAGCAATTAATGGGTAAATTCTTTTCCCATCTCTACCATTCGTTCCGCTCATCCACGTATATGTCAATCTGCAACGCAACCTTTTTTTCCCTTTTGCAGAAGACCAGACATTACTGAAGGGTTGAAAAGGCTCCGGTGTCGCACTCTCATTTTCGTCGGGGATAGCTCGCCTTTCCATTCTGAAGCACTCCACATGATATCAAAATTGGACAGAAGATATAGCGCCTTGGTTGAGGTATGACATGATAGTAATTGAACGCTCTGCAGCCACAATTGCCTAGGCTTCGTTAAATAATTAACCCGAGGGGGGGGGGGGTGATTCACGCAGGTCCAAGCTTGTGGATCGATGGTAACTGAAGAGCAGCCACACGCAATGTTGATACCAATGGAGTACTTCTTCATGGGATACGGGTTGTATAGACCATGCCAATTCAGCGACAGTCCACGGAGTCCGCTCAGTCCATCTTGCATCTCCCCAGAGCTCCTTTCTCCAGAAAGCATGGGATTAAAGCTAAAACCAATAAAGACCCGCATTTCTTAATCATCGAGGAGGGACAGAGAACTGGAAAAGAAGGAGAAGAAGGGGCTGAGCTAAGGCGAATGCTTTGCTGTGATTCGAAAGTCTGTCCGTCCGTCCGTCTGTACGTCTGTAAGTTTTTTCGTTTCCAGAATTTTGATTTGTTGAAAGGGCGTTTGTAGATAGGGAGAGATGTGAGTGAAAAAAGAGCTTACAATGATGCTGGGTAAATAACTATAGGGAGGACACATTCATTCTTTTGTAGTCATCAGACATAAAAGAGATCATATTCTTGAACTCTTTGGATTCGTATATTCTTCAATCTGACCAAAATATTTATTTATTATGACTTTCAATGAATTGAGCTGCTGCATGCTGCTACTCTTCTTCTTATGAGTAATATCATCGTTTTCTTTCATTCAACACGAATTTGTGTGTTATCTACTTAGCTATGTTCAGATGGGTTCATCTTTAGTTATATTGGTGTGTGTATGTCTGTTTTATATTCTAATATATTGATTGAATGAGATGGAGCCTGAAAATGATTGAAATGGCTAGCTGGGATTCACTGAACGAGGAGTTTTTTGCTTAATTTTTGCCGGCCTTCAACGTGATTTCCGCTTTCTCTTTTAAAAATAGTTGTGAAAGCATTCATGTTCTGTGCTTGTTGGCTATTATTATTACTATTATTATTCCCTTTATTTATGCGGCTGTCCATAAAGTCATGAGCATCATACAGGTTCTTAATGGTCACTATTGATTGCTCCCAACACCATTTTGCTTTGGTTTATTGATAAATTTGTGTAATTTGTTAAATCATACGTACTTGAAAATATAAAATG

mRNA sequence

ATGAGAGAAAGAGATTCCAACGAATCCAAAGCCTATGATTCTGGAAGAAGAGAGACGAGAGTAAAAGCGAGCGAGACAGAGACACAGGAGGACCTCCGCGACCGACGGCGACCGGCTGTTTTGGTGGTAGCGCTATCGGACGGACACAGAGCTTCGGCCATGGCAGATTCTAACGACTCTGTTTCCGTCGACATGGAGAAGATCTATCTCGGTGGAACGGAGCATCATATACGAACTGGCCAGGGTTCTGTGTCTGTTATATTAGGAGCTGCTGAGATTTGTCAGGATAATCCTATGCCCTCTGCTGATGATTTAGCAGATCAAATCCTTGAGGTTCTCAACTATTTTGGACTTGGTGCAGTGATGTGCATGGGGGTAACAGCAGGCGCTTACATTCTTACGCTTTTTGCTGTACGTTCATCTCATTGTGAAATTTATTGTGAAGCTCAATCAAAATTGAAATATAGAGAACGTGTTCTTGGTTTAATTCTTATATCCCCCTTATGCAAGGCACCGTCTTGGTCCGAGTGGTTCTATAATAAGGTAATGTCTAACTTGTTATACTTCTATGGCATGTGCGGGTTGTTGAAAGAGTGCTTGCTTCAGCGGTACTTCAGCAAGGAGGTCCGTGGTAGTGCCGAAGTTGCAGAGTCCGATATAGTTCAAGCTTGTAGAAAATTGTTAGATGAGAGACAGAGCAACAATGTTTTGAGGTTCCTCCAAGCAATTAATGGAAGACCAGACATTACTGAAGGGTTGAAAAGGCTCCGGTGTCGCACTCTCATTTTCGTCGGGGATAGCTCGCCTTTCCATTCTGAAGCACTCCACATGATATCAAAATTGGACAGAAGATATAGCGCCTTGGTTGAGGTCCAAGCTTGTGGATCGATGGTAACTGAAGAGCAGCCACACGCAATGTTGATACCAATGGAGTACTTCTTCATGGGATACGGGTTGTATAGACCATGCCAATTCAGCGACAGTCCACGGAGTCCGCTCAGTCCATCTTGCATCTCCCCAGAGCTCCTTTCTCCAGAAAGCATGGGATTAAAGCTAAAACCAATAAAGACCCGCATTTCTTAATCATCGAGGAGGGACAGAGAACTGGAAAAGAAGGAGAAGAAGGGGCTGAGCTAAGGCGAATGCTTTGCTGTGATTCGAAAGTCTGTCCGTCCGTCCGTCTGTACGTCTGTAAGTTTTTTCGTTTCCAGAATTTTGATTTGTTGAAAGGGCGTTTGTAGATAGGGAGAGATGTGAGTGAAAAAAGAGCTTACAATGATGCTGGGTAAATAACTATAGGGAGGACACATTCATTCTTTTGTAGTCATCAGACATAAAAGAGATCATATTCTTGAACTCTTTGGATTCGTATATTCTTCAATCTGACCAAAATATTTATTTATTATGACTTTCAATGAATTGAGCTGCTGCATGCTGCTACTCTTCTTCTTATGAGTAATATCATCGTTTTCTTTCATTCAACACGAATTTGTGTGTTATCTACTTAGCTATGTTCAGATGGGTTCATCTTTAGTTATATTGGTGTGTGTATGTCTGTTTTATATTCTAATATATTGATTGAATGAGATGGAGCCTGAAAATGATTGAAATGGCTAGCTGGGATTCACTGAACGAGGAGTTTTTTGCTTAATTTTTGCCGGCCTTCAACGTGATTTCCGCTTTCTCTTTTAAAAATAGTTGTGAAAGCATTCATGTTCTGTGCTTGTTGGCTATTATTATTACTATTATTATTCCCTTTATTTATGCGGCTGTCCATAAAGTCATGAGCATCATACAGGTTCTTAATGGTCACTATTGATTGCTCCCAACACCATTTTGCTTTGGTTTATTGATAAATTTGTGTAATTTGTTAAATCATACGTACTTGAAAATATAAAATG

Coding sequence (CDS)

ATGAGAGAAAGAGATTCCAACGAATCCAAAGCCTATGATTCTGGAAGAAGAGAGACGAGAGTAAAAGCGAGCGAGACAGAGACACAGGAGGACCTCCGCGACCGACGGCGACCGGCTGTTTTGGTGGTAGCGCTATCGGACGGACACAGAGCTTCGGCCATGGCAGATTCTAACGACTCTGTTTCCGTCGACATGGAGAAGATCTATCTCGGTGGAACGGAGCATCATATACGAACTGGCCAGGGTTCTGTGTCTGTTATATTAGGAGCTGCTGAGATTTGTCAGGATAATCCTATGCCCTCTGCTGATGATTTAGCAGATCAAATCCTTGAGGTTCTCAACTATTTTGGACTTGGTGCAGTGATGTGCATGGGGGTAACAGCAGGCGCTTACATTCTTACGCTTTTTGCTGTACGTTCATCTCATTGTGAAATTTATTGTGAAGCTCAATCAAAATTGAAATATAGAGAACGTGTTCTTGGTTTAATTCTTATATCCCCCTTATGCAAGGCACCGTCTTGGTCCGAGTGGTTCTATAATAAGGTAATGTCTAACTTGTTATACTTCTATGGCATGTGCGGGTTGTTGAAAGAGTGCTTGCTTCAGCGGTACTTCAGCAAGGAGGTCCGTGGTAGTGCCGAAGTTGCAGAGTCCGATATAGTTCAAGCTTGTAGAAAATTGTTAGATGAGAGACAGAGCAACAATGTTTTGAGGTTCCTCCAAGCAATTAATGGAAGACCAGACATTACTGAAGGGTTGAAAAGGCTCCGGTGTCGCACTCTCATTTTCGTCGGGGATAGCTCGCCTTTCCATTCTGAAGCACTCCACATGATATCAAAATTGGACAGAAGATATAGCGCCTTGGTTGAGGTCCAAGCTTGTGGATCGATGGTAACTGAAGAGCAGCCACACGCAATGTTGATACCAATGGAGTACTTCTTCATGGGATACGGGTTGTATAGACCATGCCAATTCAGCGACAGTCCACGGAGTCCGCTCAGTCCATCTTGCATCTCCCCAGAGCTCCTTTCTCCAGAAAGCATGGGATTAAAGCTAAAACCAATAAAGACCCGCATTTCTTAA

Protein sequence

MRERDSNESKAYDSGRRETRVKASETETQEDLRDRRRPAVLVVALSDGHRASAMADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVILGAAEICQDNPMPSADDLADQILEVLNYFGLGAVMCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNKVMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQAINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEEQPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCISPELLSPESMGLKLKPIKTRIS
BLAST of Carg12273 vs. NCBI nr
Match: XP_022934000.1 (protein NDL1-like [Cucurbita moschata] >XP_023531009.1 protein NDL1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535.0 bits (1377), Expect = 1.9e-148
Identity = 292/359 (81.34%), Postives = 292/359 (81.34%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI-------------------------- 113
           MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI                          
Sbjct: 1   MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVIVCGDQDKPPLITYPDLALNHMSCFQG 60

Query: 114 --------------------------LGAAEICQDNPMPSADDLADQILEVLNYFGLGAV 173
                                     LGAAEICQDNPMPSADDLADQILEVLNYFGLGAV
Sbjct: 61  LFFFPEAASLLLHNFCIYHISPPGHELGAAEICQDNPMPSADDLADQILEVLNYFGLGAV 120

Query: 174 MCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNK 233
           MCMGVTAGAYILTLFA               LKYRERVLGLILISPLCKAPSWSEWFYNK
Sbjct: 121 MCMGVTAGAYILTLFA---------------LKYRERVLGLILISPLCKAPSWSEWFYNK 180

Query: 234 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 293
           VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ
Sbjct: 181 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 240

Query: 294 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 353
           AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE
Sbjct: 241 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 300

Query: 354 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 361
           QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS
Sbjct: 301 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 344

BLAST of Carg12273 vs. NCBI nr
Match: XP_023004170.1 (protein NDL1-like [Cucurbita maxima])

HSP 1 Score: 528.9 bits (1361), Expect = 1.4e-146
Identity = 289/359 (80.50%), Postives = 290/359 (80.78%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI-------------------------- 113
           MADSNDSVSV+MEKIYLGGTEHHIRTGQGSVSVI                          
Sbjct: 1   MADSNDSVSVNMEKIYLGGTEHHIRTGQGSVSVIVCGDQDKPPLITYPDLALNHMSCFQG 60

Query: 114 --------------------------LGAAEICQDNPMPSADDLADQILEVLNYFGLGAV 173
                                     LGAAEICQD PMPSADDLADQILEVLNYFGLGAV
Sbjct: 61  LFFFPEAASLLLHNFCIYHISPPGHELGAAEICQDKPMPSADDLADQILEVLNYFGLGAV 120

Query: 174 MCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNK 233
           MCMGVTAGAYILTLFA               LKYRERVLGLILISPLCKAPSWSEWFYNK
Sbjct: 121 MCMGVTAGAYILTLFA---------------LKYRERVLGLILISPLCKAPSWSEWFYNK 180

Query: 234 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 293
           VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFL 
Sbjct: 181 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLH 240

Query: 294 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 353
           AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE
Sbjct: 241 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 300

Query: 354 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 361
           QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS
Sbjct: 301 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 344

BLAST of Carg12273 vs. NCBI nr
Match: XP_022147733.1 (pollen-specific protein SF21-like isoform X3 [Momordica charantia])

HSP 1 Score: 525.4 bits (1352), Expect = 1.5e-145
Identity = 286/359 (79.67%), Postives = 289/359 (80.50%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI-------------------------- 113
           MADSNDSVSVDMEKIYLGG EHH+RTGQGSVSVI                          
Sbjct: 1   MADSNDSVSVDMEKIYLGGKEHHVRTGQGSVSVIVCGDQDKPPLITYPDLALNHMSCFQG 60

Query: 114 --------------------------LGAAEICQDNPMPSADDLADQILEVLNYFGLGAV 173
                                     LGAAEICQD+P PSADDLADQILEVLNYFGLGAV
Sbjct: 61  LFFFPEAASLLLHNFCIYHISPPGHELGAAEICQDDPSPSADDLADQILEVLNYFGLGAV 120

Query: 174 MCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNK 233
           MCMGVTAGAYILTLFA               LKYRERVLGLILISPLCK+PSWSEWFYNK
Sbjct: 121 MCMGVTAGAYILTLFA---------------LKYRERVLGLILISPLCKSPSWSEWFYNK 180

Query: 234 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 293
           VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNV RFLQ
Sbjct: 181 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVFRFLQ 240

Query: 294 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 353
           AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE
Sbjct: 241 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 300

Query: 354 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 361
           QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS
Sbjct: 301 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 344

BLAST of Carg12273 vs. NCBI nr
Match: XP_022147732.1 (pollen-specific protein SF21-like isoform X2 [Momordica charantia])

HSP 1 Score: 524.2 bits (1349), Expect = 3.4e-145
Identity = 286/362 (79.01%), Postives = 289/362 (79.83%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI-------------------------- 113
           MADSNDSVSVDMEKIYLGG EHH+RTGQGSVSVI                          
Sbjct: 1   MADSNDSVSVDMEKIYLGGKEHHVRTGQGSVSVIVCGDQDKPPLITYPDLALNHMSCFQG 60

Query: 114 -----------------------------LGAAEICQDNPMPSADDLADQILEVLNYFGL 173
                                        LGAAEICQD+P PSADDLADQILEVLNYFGL
Sbjct: 61  LFFFPEAASLLLHNFCIYHISPPGHEFLQLGAAEICQDDPSPSADDLADQILEVLNYFGL 120

Query: 174 GAVMCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWF 233
           GAVMCMGVTAGAYILTLFA               LKYRERVLGLILISPLCK+PSWSEWF
Sbjct: 121 GAVMCMGVTAGAYILTLFA---------------LKYRERVLGLILISPLCKSPSWSEWF 180

Query: 234 YNKVMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLR 293
           YNKVMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNV R
Sbjct: 181 YNKVMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVFR 240

Query: 294 FLQAINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMV 353
           FLQAINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMV
Sbjct: 241 FLQAINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMV 300

Query: 354 TEEQPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTR 361
           TEEQPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTR
Sbjct: 301 TEEQPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTR 347

BLAST of Carg12273 vs. NCBI nr
Match: XP_004135699.1 (PREDICTED: pollen-specific protein SF21 isoform X1 [Cucumis sativus])

HSP 1 Score: 521.2 bits (1341), Expect = 2.9e-144
Identity = 284/359 (79.11%), Postives = 289/359 (80.50%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI-------------------------- 113
           MADSNDSVSVDMEKIYLGG EHH+RTGQGSVSVI                          
Sbjct: 1   MADSNDSVSVDMEKIYLGGKEHHVRTGQGSVSVIVCGDQDKPPLITYPDLALNHMSCFQG 60

Query: 114 --------------------------LGAAEICQDNPMPSADDLADQILEVLNYFGLGAV 173
                                     LGAAEIC+D+P PSADDLADQILEVLNYFGLGAV
Sbjct: 61  LFFFPEAASLLLHNFCIYHISPPGHELGAAEICEDDPSPSADDLADQILEVLNYFGLGAV 120

Query: 174 MCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNK 233
           MCMGVTAGAYIL+LFA               LKYRERVLGLILISPLCK+PSWSEWFYNK
Sbjct: 121 MCMGVTAGAYILSLFA---------------LKYRERVLGLILISPLCKSPSWSEWFYNK 180

Query: 234 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 293
           VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ
Sbjct: 181 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 240

Query: 294 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 353
           AIN RPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE
Sbjct: 241 AINRRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 300

Query: 354 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 361
           QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS
Sbjct: 301 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 344

BLAST of Carg12273 vs. TAIR10
Match: AT2G19620.1 (N-MYC downregulated-like 3)

HSP 1 Score: 396.4 bits (1017), Expect = 1.9e-110
Identity = 214/358 (59.78%), Postives = 255/358 (71.23%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVIL------------------------- 113
           M   N++VS+D+E+I  GG EHH++T  GSVSV++                         
Sbjct: 1   MVGLNNAVSLDIEEICNGGKEHHVKTCHGSVSVVVYGDQEKPALITYPDVALNYMSCFQG 60

Query: 114 ---------------------------GAAEICQDNPMPSADDLADQILEVLNYFGLGAV 173
                                      GAA +C ++P PS +DLADQILEVLN+F L AV
Sbjct: 61  LFLCPEAVSLLLHNFCIYHISPPGHEFGAAPVCSNDPSPSVEDLADQILEVLNFFSLEAV 120

Query: 174 MCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNK 233
           MCMG+TAGAYIL+LFA+               K++ERVLGLILISPLCKAPSWSEWFY K
Sbjct: 121 MCMGITAGAYILSLFAI---------------KHKERVLGLILISPLCKAPSWSEWFYYK 180

Query: 234 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 293
           V+SNLLY+YGM GLLK+  LQRYFSKE RGS+EV E D+V  CR+LL ER  ++++RFL+
Sbjct: 181 VVSNLLYYYGMSGLLKDIFLQRYFSKEARGSSEVPERDVVHECRRLLGERHGSSLMRFLE 240

Query: 294 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 353
           A+N R D+T+GLK L+CRTLIFVGD SPFHSE LHM++ LDR+YSALVEVQACGSMVTEE
Sbjct: 241 AVNRRHDLTDGLKSLKCRTLIFVGDQSPFHSETLHMVTALDRKYSALVEVQACGSMVTEE 300

Query: 354 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRI 360
           QPHAMLIPME+FFMG+GLYRP + SDSPRSPLSPS XXXXXXXXXX GLKLKPIKTR+
Sbjct: 301 QPHAMLIPMEFFFMGFGLYRPGRVSDSPRSPLSPSXXXXXXXXXXXXGLKLKPIKTRV 343

BLAST of Carg12273 vs. TAIR10
Match: AT5G56750.1 (N-MYC downregulated-like 1)

HSP 1 Score: 389.0 bits (998), Expect = 3.1e-108
Identity = 218/359 (60.72%), Postives = 247/359 (68.80%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI-------------------------- 113
           M DS  +VSVD+  IYLGG EH ++T  G VSVI                          
Sbjct: 1   MTDSYGAVSVDVGTIYLGGKEHRVKTASGVVSVIVYGDREKPALITYPDLALNHMSCFQG 60

Query: 114 --------------------------LGAAEICQDNPMPSADDLADQILEVLNYFGLGAV 173
                                     LGAA IC ++ +PSA++LADQILEVLN+FGLG V
Sbjct: 61  LFFCPEAASLLLHNFCIYHISPPGHELGAAPICPNDSVPSAENLADQILEVLNFFGLGVV 120

Query: 174 MCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNK 233
           MCMGVTAGAYILTLFA               +K+RERVLGLIL+SPLCKAPSWSEWFYNK
Sbjct: 121 MCMGVTAGAYILTLFA---------------MKHRERVLGLILVSPLCKAPSWSEWFYNK 180

Query: 234 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 293
           V++NLLY+YGMCG++KE LLQRYFSKEVRG+ E+ ESDI QACR+LLDERQ  NVLRFL 
Sbjct: 181 VITNLLYYYGMCGVVKEFLLQRYFSKEVRGNVEIPESDIAQACRRLLDERQGINVLRFLD 240

Query: 294 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 353
           AI+ RPDI+ GLK+L+CRTLIF+GD SPF+SEA+HM + LDR Y ALVEVQACGSMVTEE
Sbjct: 241 AIDRRPDISSGLKKLKCRTLIFIGDQSPFYSEAVHMAATLDRGYCALVEVQACGSMVTEE 300

Query: 354 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 361
           QPHAMLIPMEYF MGYGLYRP  F            XXXXXXXXXX  LKLKPIKTRIS
Sbjct: 301 QPHAMLIPMEYFLMGYGLYRPSLFXXXXXXXXXXXXXXXXXXXXXXXXLKLKPIKTRIS 344

BLAST of Carg12273 vs. TAIR10
Match: AT5G11790.1 (N-MYC downregulated-like 2)

HSP 1 Score: 388.3 bits (996), Expect = 5.3e-108
Identity = 209/359 (58.22%), Postives = 243/359 (67.69%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI-------------------------- 113
           MADS+DSVS+DME + LGG EH + T  G V V                           
Sbjct: 1   MADSSDSVSIDMEALSLGGQEHLVETTYGPVCVAVCGDPDKPALITYPDIALNYMFCFQG 60

Query: 114 --------------------------LGAAEICQDNPMPSADDLADQILEVLNYFGLGAV 173
                                     LGA  I  D P+ SADDLADQI+EVLNYFGLGAV
Sbjct: 61  LLFCPEASSLLLHNFCIYHISPLGHELGAPMISVDAPLLSADDLADQIVEVLNYFGLGAV 120

Query: 174 MCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNK 233
           MCMGVTAGAYILTLFA               +KYR+RVLGLIL+SPLC+APSWSEW  NK
Sbjct: 121 MCMGVTAGAYILTLFA---------------MKYRQRVLGLILVSPLCQAPSWSEWLCNK 180

Query: 234 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 293
           VMSNLLY+YG CG++KE LL+RYFSKEVRG+  V ESDIVQ CR+LL ERQS NV RFL+
Sbjct: 181 VMSNLLYYYGTCGVVKEMLLKRYFSKEVRGNGHVPESDIVQECRRLLSERQSTNVWRFLE 240

Query: 294 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 353
           AINGR D++EGL++L+CRTLIF+G++S +HSEA+HM +KLDRRY ALVEVQ  GS+V+EE
Sbjct: 241 AINGRVDLSEGLRKLQCRTLIFIGENSAYHSEAVHMTTKLDRRYGALVEVQGSGSLVSEE 300

Query: 354 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 361
           QP AM+IPMEYF MGYGLYRP Q S SPRSPLSP+           MGLKLKPIKTR++
Sbjct: 301 QPQAMIIPMEYFLMGYGLYRPTQ-SVSPRSPLSPTRISPELLSPENMGLKLKPIKTRLA 343

BLAST of Carg12273 vs. Swiss-Prot
Match: sp|Q9ZUN1|NDL3_ARATH (Protein NDL3 OS=Arabidopsis thaliana OX=3702 GN=NDL3 PE=1 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 3.5e-109
Identity = 214/358 (59.78%), Postives = 255/358 (71.23%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVIL------------------------- 113
           M   N++VS+D+E+I  GG EHH++T  GSVSV++                         
Sbjct: 1   MVGLNNAVSLDIEEICNGGKEHHVKTCHGSVSVVVYGDQEKPALITYPDVALNYMSCFQG 60

Query: 114 ---------------------------GAAEICQDNPMPSADDLADQILEVLNYFGLGAV 173
                                      GAA +C ++P PS +DLADQILEVLN+F L AV
Sbjct: 61  LFLCPEAVSLLLHNFCIYHISPPGHEFGAAPVCSNDPSPSVEDLADQILEVLNFFSLEAV 120

Query: 174 MCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNK 233
           MCMG+TAGAYIL+LFA+               K++ERVLGLILISPLCKAPSWSEWFY K
Sbjct: 121 MCMGITAGAYILSLFAI---------------KHKERVLGLILISPLCKAPSWSEWFYYK 180

Query: 234 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 293
           V+SNLLY+YGM GLLK+  LQRYFSKE RGS+EV E D+V  CR+LL ER  ++++RFL+
Sbjct: 181 VVSNLLYYYGMSGLLKDIFLQRYFSKEARGSSEVPERDVVHECRRLLGERHGSSLMRFLE 240

Query: 294 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 353
           A+N R D+T+GLK L+CRTLIFVGD SPFHSE LHM++ LDR+YSALVEVQACGSMVTEE
Sbjct: 241 AVNRRHDLTDGLKSLKCRTLIFVGDQSPFHSETLHMVTALDRKYSALVEVQACGSMVTEE 300

Query: 354 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRI 360
           QPHAMLIPME+FFMG+GLYRP + SDSPRSPLSPS XXXXXXXXXX GLKLKPIKTR+
Sbjct: 301 QPHAMLIPMEFFFMGFGLYRPGRVSDSPRSPLSPSXXXXXXXXXXXXGLKLKPIKTRV 343

BLAST of Carg12273 vs. Swiss-Prot
Match: sp|Q9FJT7|NDL1_ARATH (Protein NDL1 OS=Arabidopsis thaliana OX=3702 GN=NDL1 PE=1 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 5.6e-107
Identity = 218/359 (60.72%), Postives = 247/359 (68.80%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI-------------------------- 113
           M DS  +VSVD+  IYLGG EH ++T  G VSVI                          
Sbjct: 1   MTDSYGAVSVDVGTIYLGGKEHRVKTASGVVSVIVYGDREKPALITYPDLALNHMSCFQG 60

Query: 114 --------------------------LGAAEICQDNPMPSADDLADQILEVLNYFGLGAV 173
                                     LGAA IC ++ +PSA++LADQILEVLN+FGLG V
Sbjct: 61  LFFCPEAASLLLHNFCIYHISPPGHELGAAPICPNDSVPSAENLADQILEVLNFFGLGVV 120

Query: 174 MCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNK 233
           MCMGVTAGAYILTLFA               +K+RERVLGLIL+SPLCKAPSWSEWFYNK
Sbjct: 121 MCMGVTAGAYILTLFA---------------MKHRERVLGLILVSPLCKAPSWSEWFYNK 180

Query: 234 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 293
           V++NLLY+YGMCG++KE LLQRYFSKEVRG+ E+ ESDI QACR+LLDERQ  NVLRFL 
Sbjct: 181 VITNLLYYYGMCGVVKEFLLQRYFSKEVRGNVEIPESDIAQACRRLLDERQGINVLRFLD 240

Query: 294 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 353
           AI+ RPDI+ GLK+L+CRTLIF+GD SPF+SEA+HM + LDR Y ALVEVQACGSMVTEE
Sbjct: 241 AIDRRPDISSGLKKLKCRTLIFIGDQSPFYSEAVHMAATLDRGYCALVEVQACGSMVTEE 300

Query: 354 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 361
           QPHAMLIPMEYF MGYGLYRP  F            XXXXXXXXXX  LKLKPIKTRIS
Sbjct: 301 QPHAMLIPMEYFLMGYGLYRPSLFXXXXXXXXXXXXXXXXXXXXXXXXLKLKPIKTRIS 344

BLAST of Carg12273 vs. Swiss-Prot
Match: sp|Q9ASU8|NDL2_ARATH (Protein NDL2 OS=Arabidopsis thaliana OX=3702 GN=NDL2 PE=1 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 9.5e-107
Identity = 209/359 (58.22%), Postives = 243/359 (67.69%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI-------------------------- 113
           MADS+DSVS+DME + LGG EH + T  G V V                           
Sbjct: 1   MADSSDSVSIDMEALSLGGQEHLVETTYGPVCVAVCGDPDKPALITYPDIALNYMFCFQG 60

Query: 114 --------------------------LGAAEICQDNPMPSADDLADQILEVLNYFGLGAV 173
                                     LGA  I  D P+ SADDLADQI+EVLNYFGLGAV
Sbjct: 61  LLFCPEASSLLLHNFCIYHISPLGHELGAPMISVDAPLLSADDLADQIVEVLNYFGLGAV 120

Query: 174 MCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNK 233
           MCMGVTAGAYILTLFA               +KYR+RVLGLIL+SPLC+APSWSEW  NK
Sbjct: 121 MCMGVTAGAYILTLFA---------------MKYRQRVLGLILVSPLCQAPSWSEWLCNK 180

Query: 234 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 293
           VMSNLLY+YG CG++KE LL+RYFSKEVRG+  V ESDIVQ CR+LL ERQS NV RFL+
Sbjct: 181 VMSNLLYYYGTCGVVKEMLLKRYFSKEVRGNGHVPESDIVQECRRLLSERQSTNVWRFLE 240

Query: 294 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 353
           AINGR D++EGL++L+CRTLIF+G++S +HSEA+HM +KLDRRY ALVEVQ  GS+V+EE
Sbjct: 241 AINGRVDLSEGLRKLQCRTLIFIGENSAYHSEAVHMTTKLDRRYGALVEVQGSGSLVSEE 300

Query: 354 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 361
           QP AM+IPMEYF MGYGLYRP Q S SPRSPLSP+           MGLKLKPIKTR++
Sbjct: 301 QPQAMIIPMEYFLMGYGLYRPTQ-SVSPRSPLSPTRISPELLSPENMGLKLKPIKTRLA 343

BLAST of Carg12273 vs. Swiss-Prot
Match: sp|O23969|SF21_HELAN (Pollen-specific protein SF21 OS=Helianthus annuus OX=4232 GN=SF21 PE=2 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 4.0e-97
Identity = 195/359 (54.32%), Postives = 225/359 (62.67%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI-------------------------- 113
           MADS  S+SV     + GG EH IRTG GSVSV                           
Sbjct: 1   MADSGHSISVTFPSFHSGGKEHIIRTGCGSVSVTVCGDQEKPPLITYPDLALNHMSCFQG 60

Query: 114 --------------------------LGAAEICQDNPMPSADDLADQILEVLNYFGLGAV 173
                                     LGAA I  D+P+PS +DL DQIL VLNYF LG+V
Sbjct: 61  LFVSPESASLLLHNFCIYHINPPGHELGAASIGIDDPVPSIEDLCDQILVVLNYFRLGSV 120

Query: 174 MCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNK 233
           MCMG  AGAYILTLF++               KY ERV GLILISP+CKAPSW+E FYNK
Sbjct: 121 MCMGAMAGAYILTLFSI---------------KYSERVTGLILISPICKAPSWTERFYNK 180

Query: 234 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 293
           + S  LY+YGMC L+KE L+ RYFSKEV G+ E+ ESD+V ACRKLLDER S NV R+LQ
Sbjct: 181 LTSKTLYYYGMCDLVKELLIHRYFSKEVCGNPEIPESDMVLACRKLLDERDSVNVWRYLQ 240

Query: 294 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 353
           AI+ R DITE LK L C+T+IFVGDSSPFH EAL +  KL    SALVEV ACGSMVT+E
Sbjct: 241 AIDSRRDITEELKSLECKTIIFVGDSSPFHDEALQIAEKLGTNCSALVEVHACGSMVTQE 300

Query: 354 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 361
           QPHAMLIP+E F  G+GLYRPC++S+SPRSPL PS           MGLKL+PIK R+S
Sbjct: 301 QPHAMLIPLENFLKGFGLYRPCRYSNSPRSPLGPSSIDPELLYPEKMGLKLRPIKLRVS 344

BLAST of Carg12273 vs. Swiss-Prot
Match: sp|Q641F2|NDR1A_XENLA (Protein NDRG1-A OS=Xenopus laevis OX=8355 GN=ndrg1-a PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 4.8e-18
Identity = 74/242 (30.58%), Postives = 106/242 (43.80%), Query Frame = 0

Query: 89  GAAEICQDNPMPSADDLADQILEVLNYFGLGAVMCMGVTAGAYILTLFAVRSSHCEIYCE 148
           GAA        PS D LA+ +  V+   GL  VM +G+ AGAYILT FA           
Sbjct: 103 GAASFPAGYMYPSMDQLAEMLPGVVQQLGLRTVMGLGIGAGAYILTRFA----------- 162

Query: 149 AQSKLKYRERVLGLILISPLCKAPSWSEWFYNKVMSNLLYFYGMCGLLKECLLQRYFSK- 208
               L +   V GL+LI+    A  W +W   K+        G    L + ++   FSK 
Sbjct: 163 ----LNHPSMVEGLVLININPCAEGWMDWAATKIS-------GWTNALPDMVISHLFSKD 222

Query: 209 EVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQAINGRPDIT-------EGLKRLRCRT 268
           EV  + E+ E+      + +L +   NNV  F+++ N R D+             L+C  
Sbjct: 223 EVHSNPELVET----YRQHILHDINQNNVQHFVKSYNSRRDLEIERPIPGTNAVTLKCPA 282

Query: 269 LIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEEQPHAMLIPMEYFFMGYGLY 323
           L+ VGDSSP     +   SKLD   + L+++  CG      QP  +    +YF  G G Y
Sbjct: 283 LLVVGDSSPAVDAVVECNSKLDPTKTTLLKMSDCGGFPQVIQPAKLAEAFKYFVQGMG-Y 317

BLAST of Carg12273 vs. TrEMBL
Match: tr|A0A1S3BQ33|A0A1S3BQ33_CUCME (pollen-specific protein SF21 OS=Cucumis melo OX=3656 GN=LOC103492303 PE=4 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 1.4e-142
Identity = 280/359 (77.99%), Postives = 288/359 (80.22%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI-------------------------- 113
           MADSNDSVSVDMEKIYLGG EHH++TG+GSVSVI                          
Sbjct: 1   MADSNDSVSVDMEKIYLGGKEHHVQTGRGSVSVIVCGDQDKPPLITYPDLALNHMSCFQG 60

Query: 114 --------------------------LGAAEICQDNPMPSADDLADQILEVLNYFGLGAV 173
                                     LGA EIC+D+P PSADDLADQILEVLN+FGLGAV
Sbjct: 61  LFFFPEAASLLLHNFCIYHISPPGHELGAGEICEDDPSPSADDLADQILEVLNFFGLGAV 120

Query: 174 MCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNK 233
           MCMGVTAGAYIL+LFA               LKYRERVLGLILISPLCK+PSWSEWFYNK
Sbjct: 121 MCMGVTAGAYILSLFA---------------LKYRERVLGLILISPLCKSPSWSEWFYNK 180

Query: 234 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 293
           VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ
Sbjct: 181 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 240

Query: 294 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 353
           AIN RPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE
Sbjct: 241 AINRRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 300

Query: 354 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 361
           QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS
Sbjct: 301 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 344

BLAST of Carg12273 vs. TrEMBL
Match: tr|A0A0A0LZC6|A0A0A0LZC6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G586790 PE=4 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 2.0e-138
Identity = 274/333 (82.28%), Postives = 283/333 (84.98%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGT-----EHHIRTGQG------SVSVI--------------- 113
           MADSNDSVSVDMEKIYLGG        ++   QG      + S++               
Sbjct: 1   MADSNDSVSVDMEKIYLGGKVSFLFSKNMSCFQGLFFFPEAASLLLHNFCIYHISPPGHE 60

Query: 114 LGAAEICQDNPMPSADDLADQILEVLNYFGLGAVMCMGVTAGAYILTLFAVRSSHCEIYC 173
           LGAAEIC+D+P PSADDLADQILEVLNYFGLGAVMCMGVTAGAYIL+LFA          
Sbjct: 61  LGAAEICEDDPSPSADDLADQILEVLNYFGLGAVMCMGVTAGAYILSLFA---------- 120

Query: 174 EAQSKLKYRERVLGLILISPLCKAPSWSEWFYNKVMSNLLYFYGMCGLLKECLLQRYFSK 233
                LKYRERVLGLILISPLCK+PSWSEWFYNKVMSNLLYFYGMCGLLKECLLQRYFSK
Sbjct: 121 -----LKYRERVLGLILISPLCKSPSWSEWFYNKVMSNLLYFYGMCGLLKECLLQRYFSK 180

Query: 234 EVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQAINGRPDITEGLKRLRCRTLIFVGDS 293
           EVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQAIN RPDITEGLKRLRCRTLIFVGDS
Sbjct: 181 EVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQAINRRPDITEGLKRLRCRTLIFVGDS 240

Query: 294 SPFHSEALHMISKLDRRYSALVEVQACGSMVTEEQPHAMLIPMEYFFMGYGLYRPCQFSD 353
           SPFHSEALHMISKLDRRYSALVEVQACGSMVTEEQPHAMLIPMEYFFMGYGLYRPCQFSD
Sbjct: 241 SPFHSEALHMISKLDRRYSALVEVQACGSMVTEEQPHAMLIPMEYFFMGYGLYRPCQFSD 300

Query: 354 SPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 361
           SPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS
Sbjct: 301 SPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 318

BLAST of Carg12273 vs. TrEMBL
Match: tr|M5W2N3|M5W2N3_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G213100 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 3.4e-133
Identity = 263/359 (73.26%), Postives = 279/359 (77.72%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI-------------------------- 113
           MA+SND+VS+DMEKIYLGG EH IRTG GSVSVI                          
Sbjct: 1   MAESNDAVSLDMEKIYLGGKEHFIRTGCGSVSVIVYGDQEKPALITYPDLALNHMSCFQG 60

Query: 114 --------------------------LGAAEICQDNPMPSADDLADQILEVLNYFGLGAV 173
                                     LGAA I  D+P+PS DDLADQILEVLN+FGLGAV
Sbjct: 61  LFFCPEAASLLLHNFCIYHISPPGHELGAASIFPDDPVPSVDDLADQILEVLNFFGLGAV 120

Query: 174 MCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNK 233
           MCMGVTAGAYIL+LFA               +KYRERVLGLIL+SPLCK+PSW+EWFYNK
Sbjct: 121 MCMGVTAGAYILSLFA---------------MKYRERVLGLILVSPLCKSPSWTEWFYNK 180

Query: 234 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 293
           VMSN+LYFYGMCGLLKECLLQRYFSKEVRGSAEV ESDIVQACRKLL+ERQS+NV RFLQ
Sbjct: 181 VMSNMLYFYGMCGLLKECLLQRYFSKEVRGSAEVPESDIVQACRKLLEERQSSNVFRFLQ 240

Query: 294 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 353
           AIN RPDITEGLK LRCRTLIFVGDSSPFHSEALHM +KLDRRYSALVEVQACGSMVTEE
Sbjct: 241 AINRRPDITEGLKSLRCRTLIFVGDSSPFHSEALHMTAKLDRRYSALVEVQACGSMVTEE 300

Query: 354 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 361
           QPHAMLIPMEYFFMGYGLYRPC FSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTR+S
Sbjct: 301 QPHAMLIPMEYFFMGYGLYRPCHFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRVS 344

BLAST of Carg12273 vs. TrEMBL
Match: tr|A0A2N9G507|A0A2N9G507_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS22437 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 2.2e-132
Identity = 263/359 (73.26%), Postives = 277/359 (77.16%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI-------------------------- 113
           MADSNDS SVDMEKI+LGG EH IRTG GSVSVI                          
Sbjct: 1   MADSNDSFSVDMEKIHLGGKEHLIRTGCGSVSVIVYGDQDKPALITYPDLALNHVSCFQG 60

Query: 114 --------------------------LGAAEICQDNPMPSADDLADQILEVLNYFGLGAV 173
                                     LGAA IC ++P+PS DDLADQI+EVLN+FGLGAV
Sbjct: 61  LFFCPEAASLLLHNFCIYHISPPGHELGAAAICPEDPLPSVDDLADQIIEVLNFFGLGAV 120

Query: 174 MCMGVTAGAYILTLFAVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNK 233
           MCMGVTAGAYILTLFA               LKYRERVLGLIL+SPLCKAPSWSEWFYNK
Sbjct: 121 MCMGVTAGAYILTLFA---------------LKYRERVLGLILVSPLCKAPSWSEWFYNK 180

Query: 234 VMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQ 293
           +MSNLLYFYGMCGLLKECLLQRYFSKEVRGSA V ESDIVQAC++LLDERQ+ NV RFLQ
Sbjct: 181 LMSNLLYFYGMCGLLKECLLQRYFSKEVRGSAGVPESDIVQACKRLLDERQAINVFRFLQ 240

Query: 294 AINGRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEE 353
           AIN RPDITEGLKRLRCRTLIFVGDSSPFHSEALHM SKLDRR+SALVEVQACGSMVTEE
Sbjct: 241 AINRRPDITEGLKRLRCRTLIFVGDSSPFHSEALHMTSKLDRRFSALVEVQACGSMVTEE 300

Query: 354 QPHAMLIPMEYFFMGYGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 361
           QPHAMLIPME+F MGYGLYRPC FSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTR+S
Sbjct: 301 QPHAMLIPMEFFLMGYGLYRPCHFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRVS 344

BLAST of Carg12273 vs. TrEMBL
Match: tr|A0A2K1WWY8|A0A2K1WWY8_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_018G067700v3 PE=4 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 3.7e-132
Identity = 258/344 (75.00%), Postives = 274/344 (79.65%), Query Frame = 0

Query: 54  MADSNDSVSVDMEKIYLGGTEHHIRTGQGSVSVI-------------------------- 113
           MA+SNDSV VD+EKIYLGG EHH+RTG GSVSVI                          
Sbjct: 1   MAESNDSVFVDVEKIYLGGKEHHVRTGCGSVSVIVYGDQDKPALITYPDLALNYMSCFQG 60

Query: 114 -----------LGAAEICQDNPMPSADDLADQILEVLNYFGLGAVMCMGVTAGAYILTLF 173
                      LGAA I  D+P+PS DDLADQI+EVLNYFGLGAVMCMGVTAGAYILTLF
Sbjct: 61  LFFCPEAASLLLGAAPISPDDPLPSVDDLADQIIEVLNYFGLGAVMCMGVTAGAYILTLF 120

Query: 174 AVRSSHCEIYCEAQSKLKYRERVLGLILISPLCKAPSWSEWFYNKVMSNLLYFYGMCGLL 233
           A               +KYR+RVLGLIL+SPLCK PSW+EW YNKVMSNLLYFYGMCGLL
Sbjct: 121 A---------------MKYRQRVLGLILVSPLCKTPSWTEWLYNKVMSNLLYFYGMCGLL 180

Query: 234 KECLLQRYFSKEVRGSAEVAESDIVQACRKLLDERQSNNVLRFLQAINGRPDITEGLKRL 293
           KE LLQRYFSK+VRGSAEV ESDI QACR LLDERQ  NVLRFLQAIN RPDIT GLK+L
Sbjct: 181 KEFLLQRYFSKDVRGSAEVPESDIAQACRGLLDERQGINVLRFLQAINQRPDITSGLKKL 240

Query: 294 RCRTLIFVGDSSPFHSEALHMISKLDRRYSALVEVQACGSMVTEEQPHAMLIPMEYFFMG 353
           RCRTL+FVGD+SPFHSEALHMI+KLDRRYSALVEVQACGSMVTEEQPHAMLIPMEYFFMG
Sbjct: 241 RCRTLVFVGDNSPFHSEALHMITKLDRRYSALVEVQACGSMVTEEQPHAMLIPMEYFFMG 300

Query: 354 YGLYRPCQFSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRIS 361
           YGLYRPCQ SDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTR+S
Sbjct: 301 YGLYRPCQLSDSPRSPLSPSCXXXXXXXXXXMGLKLKPIKTRVS 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022934000.11.9e-14881.34protein NDL1-like [Cucurbita moschata] >XP_023531009.1 protein NDL1-like [Cucurb... [more]
XP_023004170.11.4e-14680.50protein NDL1-like [Cucurbita maxima][more]
XP_022147733.11.5e-14579.67pollen-specific protein SF21-like isoform X3 [Momordica charantia][more]
XP_022147732.13.4e-14579.01pollen-specific protein SF21-like isoform X2 [Momordica charantia][more]
XP_004135699.12.9e-14479.11PREDICTED: pollen-specific protein SF21 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT2G19620.11.9e-11059.78N-MYC downregulated-like 3[more]
AT5G56750.13.1e-10860.72N-MYC downregulated-like 1[more]
AT5G11790.15.3e-10858.22N-MYC downregulated-like 2[more]
Match NameE-valueIdentityDescription
sp|Q9ZUN1|NDL3_ARATH3.5e-10959.78Protein NDL3 OS=Arabidopsis thaliana OX=3702 GN=NDL3 PE=1 SV=1[more]
sp|Q9FJT7|NDL1_ARATH5.6e-10760.72Protein NDL1 OS=Arabidopsis thaliana OX=3702 GN=NDL1 PE=1 SV=1[more]
sp|Q9ASU8|NDL2_ARATH9.5e-10758.22Protein NDL2 OS=Arabidopsis thaliana OX=3702 GN=NDL2 PE=1 SV=1[more]
sp|O23969|SF21_HELAN4.0e-9754.32Pollen-specific protein SF21 OS=Helianthus annuus OX=4232 GN=SF21 PE=2 SV=1[more]
sp|Q641F2|NDR1A_XENLA4.8e-1830.58Protein NDRG1-A OS=Xenopus laevis OX=8355 GN=ndrg1-a PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3BQ33|A0A1S3BQ33_CUCME1.4e-14277.99pollen-specific protein SF21 OS=Cucumis melo OX=3656 GN=LOC103492303 PE=4 SV=1[more]
tr|A0A0A0LZC6|A0A0A0LZC6_CUCSA2.0e-13882.28Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G586790 PE=4 SV=1[more]
tr|M5W2N3|M5W2N3_PRUPE3.4e-13373.26Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_7G213100 PE=4 SV=1[more]
tr|A0A2N9G507|A0A2N9G507_FAGSY2.2e-13273.26Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS22437 PE=4 SV=1[more]
tr|A0A2K1WWY8|A0A2K1WWY8_POPTR3.7e-13275.00Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_018G067700v3 PE=... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004142NDRG
IPR029058AB_hydrolase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12273-RACarg12273-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3DG3DSA:3.40.50.1820coord: 69..316
e-value: 5.8E-48
score: 165.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILYSSF53474alpha/beta-Hydrolasescoord: 99..311
IPR004142NDRGPFAMPF03096Ndrcoord: 89..321
e-value: 3.6E-76
score: 255.5
IPR004142NDRGPANTHERPTHR11034N-MYC DOWNSTREAM REGULATEDcoord: 88..360
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..34
NoneNo IPR availablePANTHERPTHR11034:SF29SUBFAMILY NOT NAMEDcoord: 88..360