Carg10449 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg10449
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Cytochrome P450, chloroplastic
Location	Cucurbita_argyrosperma_scaffold_098 : 243202 .. 244281 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAATCCCATCTCCATATCCCTCCATTAAATCACCTTCCATTGCCGAAGGGAATCCCCCCACGCCCACGACCAGTGGTGCAAAATCCGGTTCTTGGGTCAGCCCTGATTGCCTCACCTCTCCGACTCGCTTCATTACTGATCTTCTCGGCCCTGCTCTTTTTCTTTCCCTCTTCAATTGGATGAATGACTATGGACCCATTTACAGGCTCGCCGCTGGCCCTAGAAATTTTGTCGTGGTTAGTGACCCCGCCATTGCTAACCACGTTCTCAGCAACTATGGGACTCACGCTAAAGGGCTTGTTTCTGATGTCTCCGAGTTCGTGTCTGGGTCGGGTTTCGCGATTGCAGAAGGCCCTCTTTGGACGGTAATCTTTTTGCTTTTGCTAACTGTTCTGTTGATGAGCAAAGACAAATTGAAAATTTATCATCGTGTATTGCATTCTTAGTAACTATTAATTTACGGTGATCAGGAGAAGTGTTGAAGAACTCATTGCAAATTGCAAAGAAATTGTGGAAACTGAGGAGGAACATGTGGATGACGCTGATCCAAGCTTCCTTTGATTTTTGCTGGCCAGTACACAAGAGGTGTGTTCTTGATAGCTTTAAAGTTTTCCATTCATTACCAACATTTTGCAAAATCAATCAAGAACCGAATACAGAATGAATACTTACATCCTTACTAAAATTGAAGTGAAAATCATTTATTTGTCTCAATCATTCTCAAGTATTCTTTTGTTGCCAGTTTGGAAATATATTCGAACATGTTCTTGCTTTGTGAAGTGTTTTGCACATTGTTGTAACTCCCACTTATGTTTCAAGGTTTGGGATGAAGCAGAGGAGTTTATTCCAGAATTATTTGACTTGGAAGGTCCTGTCCCTAATGAAAACGATACAGATTTCAGGTACATATTCTATTTGTGTGTGGAGACTACCCTGTTGATTGTTTTAAATAGTACAGTAGACACTTACCAGGCGCTCATAGCCATGTTTGGTGCGAGAAACTTGACCATAGGTTAGTCATACTCTTAAAGGCAAGCGCCAATGCACATGCTAACATGCTCAAATGGTGGCACGAGCAT

mRNA sequence

AGAATCCCATCTCCATATCCCTCCATTAAATCACCTTCCATTGCCGAAGGGAATCCCCCCACGCCCACGACCAGTGGTGCAAAATCCGGTTCTTGGGTCAGCCCTGATTGCCTCACCTCTCCGACTCGCTTCATTACTGATCTTCTCGGCCCTGCTCTTTTTCTTTCCCTCTTCAATTGGATGAATGACTATGGACCCATTTACAGGCTCGCCGCTGGCCCTAGAAATTTTGTCGTGGTTAGTGACCCCGCCATTGCTAACCACGTTCTCAGCAACTATGGGACTCACGCTAAAGGGCTTGTTTCTGATGTCTCCGAGTTCGTGTCTGGGTCGGGTTTCGCGATTGCAGAAGGCCCTCTTTGGACGGTAATCTTTTTGCTTTTGCTAACTGTTCTGTTGATGAGCAAAGACAAATTGAAAATTTATCATCGAGAAGTGTTGAAGAACTCATTGCAAATTGCAAAGAAATTGTGGAAACTGAGGAGGAACATGTGGATGACGCTGATCCAAGCTTCCTTTGATTTTTGCTGGCCAGTACACAAGAGTTTTCCATTCATTACCAACATTTTGCAAAATCAATCAAGAACCGAATACAGAATGAATACTTACATCCTTACTAAAATTGAAGTGAAAATCATTTATTTGTCTCAATCATTCTCAAGTATTCTTTTGTTGCCAGTTTGGAAATATATTCGAACATGTTCTTGCTTTGTGAAGTGTTTTGCACATTGTTGTAACTCCCACTTATGTTTCAAGGTTTGGGATGAAGCAGAGGAGTTTATTCCAGAATTATTTGACTTGGAAGGTCCTGTCCCTAATGAAAACGATACAGATTTCAGACACTTACCAGGCGCTCATAGCCATGTTTGGTGCGAGAAACTTGACCATAGGTTAGTCATACTCTTAAAGGCAAGCGCCAATGCACATGCTAACATGCTCAAATGGTGGCACGAGCAT

Coding sequence (CDS)

AGAATCCCATCTCCATATCCCTCCATTAAATCACCTTCCATTGCCGAAGGGAATCCCCCCACGCCCACGACCAGTGGTGCAAAATCCGGTTCTTGGGTCAGCCCTGATTGCCTCACCTCTCCGACTCGCTTCATTACTGATCTTCTCGGCCCTGCTCTTTTTCTTTCCCTCTTCAATTGGATGAATGACTATGGACCCATTTACAGGCTCGCCGCTGGCCCTAGAAATTTTGTCGTGGTTAGTGACCCCGCCATTGCTAACCACGTTCTCAGCAACTATGGGACTCACGCTAAAGGGCTTGTTTCTGATGTCTCCGAGTTCGTGTCTGGGTCGGGTTTCGCGATTGCAGAAGGCCCTCTTTGGACGGTAATCTTTTTGCTTTTGCTAACTGTTCTGTTGATGAGCAAAGACAAATTGAAAATTTATCATCGAGAAGTGTTGAAGAACTCATTGCAAATTGCAAAGAAATTGTGGAAACTGAGGAGGAACATGTGGATGACGCTGATCCAAGCTTCCTTTGATTTTTGCTGGCCAGTACACAAGAGTTTTCCATTCATTACCAACATTTTGCAAAATCAATCAAGAACCGAATACAGAATGAATACTTACATCCTTACTAAAATTGAAGTGAAAATCATTTATTTGTCTCAATCATTCTCAAGTATTCTTTTGTTGCCAGTTTGGAAATATATTCGAACATGTTCTTGCTTTGTGAAGTGTTTTGCACATTGTTGTAACTCCCACTTATGTTTCAAGGTTTGGGATGAAGCAGAGGAGTTTATTCCAGAATTATTTGACTTGGAAGGTCCTGTCCCTAATGAAAACGATACAGATTTCAGACACTTACCAGGCGCTCATAGCCATGTTTGGTGCGAGAAACTTGACCATAGGTTAGTCATACTCTTAAAGGCAAGCGCCAATGCACATGCTAACATGCTCAAATGGTGGCACGAGCAT

Protein sequence

RIPSPYPSIKSPSIAEGNPPTPTTSGAKSGSWVSPDCLTSPTRFITDLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHAKGLVSDVSEFVSGSGFAIAEGPLWTVIFLLLLTVLLMSKDKLKIYHREVLKNSLQIAKKLWKLRRNMWMTLIQASFDFCWPVHKSFPFITNILQNQSRTEYRMNTYILTKIEVKIIYLSQSFSSILLLPVWKYIRTCSCFVKCFAHCCNSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLPGAHSHVWCEKLDHRLVILLKASANAHANMLKWWHEH

Homology

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BLAST of Carg10449 vs. NCBI nr
Match: XP_022143881.1 (carotene epsilon-monooxygenase, chloroplastic [Momordica charantia])

HSP 1 Score: 182.6 bits (462), Expect = 2.1e-42
Identity = 142/350 (40.57%), Postives = 179/350 (51.14%), Query Frame = 0


Query: 1   RIPSPYPSIKSPSIAEGNPPTPT-----TSGAKSGSWVSPDCLTSPTRFIT--------- 60
           RIP P+ SIKS SI EGNPPTPT     T+ AKSGSWVSPD LTS TR+IT         
Sbjct: 26  RIPPPFLSIKS-SIDEGNPPTPTKLKNSTNNAKSGSWVSPDWLTSLTRYITLGQGDDSGI 85

Query: 61  -----------DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGT 120
                      DLLG ALFL LF WMNDYGPIYRLAAGPRNFVVVSDPAIA HVL NYGT
Sbjct: 86  PIASAKLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGT 145

Query: 121 HAKGLVSDVSEFVSGSGFAIAEGPLWTVIFLLLLTVLLMSKDKLKIYHREVLKNSLQIAK 180
           +AKGLVS+VSEF+ GSGFAIAEGPLWTV    ++   L  K    I  R   K ++++ +
Sbjct: 146 YAKGLVSEVSEFLFGSGFAIAEGPLWTVRRRAVVP-SLHKKYLSVIVDRVFCKCAMRLVE 205

Query: 181 KLWKLRRNMWMTLIQASFDFCWPVHKSFPFITNILQNQSRTEYRMNTYILTKIEVKIIYL 240
           KL K           A  +    + + F  +T  +   S   Y  ++       +  +Y 
Sbjct: 206 KLQK----------DALNNNSVNMEEKFSQLTLDIIGLSVFNYSFDSLSADSPVIDAVYT 265

Query: 241 S---QSFSSILLLPVWKYIRTCSCFVKCFAHCCNSHLCFKVWDEAEEFIPELFDLEGPVP 300
           +       S  +LP WK    C    +         +  +  +E      E+ + EG   
Sbjct: 266 ALKEAEARSTDILPYWKIKALCKIIPRQIKAEEAVTVIRRTVEELIAKCKEIVETEGERI 325

Query: 301 NE----NDTD---FRHLPGAHSHVWCEKLDHRLVILLKASANAHANMLKW 316
           +E    NDTD    R L  +   V   +L   L+ +L A      ++L W
Sbjct: 326 DEEEYVNDTDPSILRFLLASRQEVSSVQLRDDLLSMLVAGHETTGSVLTW 363

HSP 2 Score: 63.2 bits (152), Expect = 1.9e-06
Identity = 26/38 (68.42%), Postives = 32/38 (84.21%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   +VW++AEEFIPE FDLEGPVPNE++TDFR +P
Sbjct: 447 NIHRSSQVWEQAEEFIPERFDLEGPVPNESNTDFRFIP 484

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BLAST of Carg10449 vs. NCBI nr
Match: XP_022972774.1 (carotene epsilon-monooxygenase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 172.9 bits (437), Expect = 1.7e-39
Identity = 98/148 (66.22%), Postives = 107/148 (72.30%), Query Frame = 0


Query: 1   RIPSPYPSIKSPSIAEGNPPTP-----TTSGAKSGSWVSPDCLTSPTRFIT--------- 60
           R   P+ SIKS SI E +PPTP     +T+ +KSGSWVSPD LTS TR+IT         
Sbjct: 26  RTQFPFLSIKS-SIDERDPPTPAKLNNSTNTSKSGSWVSPDWLTSLTRYITLGQGDDSGI 85

Query: 61  -----------DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGT 120
                      DLLG ALFL LF WMNDYGPIYRLAAGPRNFVVVSDPAIA HVL NYGT
Sbjct: 86  PIASAKLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGT 145

Query: 121 HAKGLVSDVSEFVSGSGFAIAEGPLWTV 124
           +AKGLVS+VSEF+ GSGFAIAEGPLWTV
Sbjct: 146 YAKGLVSEVSEFLFGSGFAIAEGPLWTV 172

HSP 2 Score: 61.2 bits (147), Expect = 7.2e-06
Identity = 25/38 (65.79%), Postives = 31/38 (81.58%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   +VW+ AEEFIPE FDL+GPVPNE++TDFR +P
Sbjct: 447 NIHRSSQVWERAEEFIPERFDLDGPVPNESNTDFRFIP 484

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BLAST of Carg10449 vs. NCBI nr
Match: XP_008462512.1 (PREDICTED: carotene epsilon-monooxygenase, chloroplastic [Cucumis melo])

HSP 1 Score: 172.2 bits (435), Expect = 2.9e-39
Identity = 96/146 (65.75%), Postives = 101/146 (69.18%), Query Frame = 0


Query: 3   PSPYPSIKSPSIAEGNPPTP-----TTSGAKSGSWVSPDCLTSPTRFIT----------- 62
           P PYPSIKS     GNP TP      T+  KS SWVSPD LTS TR IT           
Sbjct: 28  PFPYPSIKSSLDERGNPSTPPKLKNPTNAPKSRSWVSPDWLTSLTRSITLGQGDDSGIPI 87

Query: 63  ---------DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHA 122
                    DLLG ALFL LF WMNDYGPIYRLAAGPRNFV+VSDPAIA HVL NYGT+A
Sbjct: 88  ATAKLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVIVSDPAIAKHVLRNYGTYA 147

Query: 123 KGLVSDVSEFVSGSGFAIAEGPLWTV 124
           KGLVS+VSEF+ GSGFAIAEGPLWTV
Sbjct: 148 KGLVSEVSEFLFGSGFAIAEGPLWTV 173

[Close]

BLAST of Carg10449 vs. NCBI nr
Match: XP_022962840.1 (carotene epsilon-monooxygenase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 171.8 bits (434), Expect = 3.8e-39
Identity = 97/148 (65.54%), Postives = 107/148 (72.30%), Query Frame = 0


Query: 1   RIPSPYPSIKSPSIAEGNPPTP-----TTSGAKSGSWVSPDCLTSPTRFIT--------- 60
           R   P+ SI+S SI E +PPTP     +T+ +KSGSWVSPD LTS TR+IT         
Sbjct: 26  RTQFPFLSIRS-SIDERDPPTPAKLNNSTNTSKSGSWVSPDWLTSLTRYITLGQGDDSGI 85

Query: 61  -----------DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGT 120
                      DLLG ALFL LF WMNDYGPIYRLAAGPRNFVVVSDPAIA HVL NYGT
Sbjct: 86  PIASAKLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGT 145

Query: 121 HAKGLVSDVSEFVSGSGFAIAEGPLWTV 124
           +AKGLVS+VSEF+ GSGFAIAEGPLWTV
Sbjct: 146 YAKGLVSEVSEFLFGSGFAIAEGPLWTV 172

HSP 2 Score: 62.4 bits (150), Expect = 3.2e-06
Identity = 26/38 (68.42%), Postives = 31/38 (81.58%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   +VW+ AEEFIPE FDLEGPVPNE++TDFR +P
Sbjct: 447 NIHRSSQVWERAEEFIPERFDLEGPVPNESNTDFRFIP 484

[Close]

BLAST of Carg10449 vs. NCBI nr
Match: XP_023518813.1 (carotene epsilon-monooxygenase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 170.2 bits (430), Expect = 1.1e-38
Identity = 95/141 (67.38%), Postives = 104/141 (73.76%), Query Frame = 0


Query: 8   SIKSPSIAEGNPPTP-----TTSGAKSGSWVSPDCLTSPTRFIT---------------- 67
           SI+S SI E +PPTP     +T+ +KSGSWVSPD LTS TR+IT                
Sbjct: 33  SIRS-SIDERDPPTPAKLNNSTNTSKSGSWVSPDWLTSLTRYITLGQGDDSGIPIASAKL 92

Query: 68  ----DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHAKGLVS 124
               DLLG ALFL LF WMNDYGPIYRLAAGPRNFVVVSDPAIA HVL NYGT+AKGLVS
Sbjct: 93  DDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGTYAKGLVS 152

HSP 2 Score: 63.2 bits (152), Expect = 1.9e-06
Identity = 26/38 (68.42%), Postives = 32/38 (84.21%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   +VW++AEEFIPE FDLEGPVPNE++TDFR +P
Sbjct: 447 NIHRSSQVWEQAEEFIPERFDLEGPVPNESNTDFRFIP 484

[Close]

BLAST of Carg10449 vs. TAIR10
Match: AT3G53130.1 (Cytochrome P450 superfamily protein)

HSP 1 Score: 143.3 bits (360), Expect = 2.6e-34
Identity = 79/140 (56.43%), Postives = 91/140 (65.00%), Query Frame = 0


Query: 3   PSPYPSIKSPSIAEGNPPTPTTSGAKSGSWVSPDCLTSPTRF------------------ 62
           P P  +    S  E   P   T+ +KS SWVSPD LT+ TR                   
Sbjct: 27  PKPKFTFSIRSSIEKPKPKLETNSSKSQSWVSPDWLTTLTRTLSSGKNDESGIPIANAKL 86

Query: 63  --ITDLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHAKGLVS 122
             + DLLG ALFL L+ WMN+YGPIYRLAAGPRNFV+VSDPAIA HVL NY  +AKGLV+
Sbjct: 87  DDVADLLGGALFLPLYKWMNEYGPIYRLAAGPRNFVIVSDPAIAKHVLRNYPKYAKGLVA 146

HSP 2 Score: 55.1 bits (131), Expect = 9.4e-08
Identity = 20/38 (52.63%), Postives = 30/38 (78.95%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   +VW++AEEF+PE FD++G +PNE +TDF+ +P
Sbjct: 442 NIHRSSEVWEKAEEFLPERFDIDGAIPNETNTDFKFIP 479

[Close]

BLAST of Carg10449 vs. TAIR10
Match: AT4G15110.1 (cytochrome P450, family 97, subfamily B, polypeptide 3)

HSP 1 Score: 74.3 bits (181), Expect = 1.5e-13
Identity = 35/78 (44.87%), Postives = 53/78 (67.95%), Query Frame = 0


Query: 45  ITDLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVL-SNYGTHAKGLVSD 104
           ++DL G  LFLSL++W  ++G IY+LA GP+ FVV+SDP IA HVL  N  ++ KG++++
Sbjct: 91  VSDLFGKPLFLSLYDWFLEHGGIYKLAFGPKAFVVISDPIIARHVLRENAFSYDKGVLAE 150

Query: 105 VSEFVSGSGFAIAEGPLW 122
           + E + G G   A+   W
Sbjct: 151 ILEPIMGKGLIPADLDTW 168

[Close]

BLAST of Carg10449 vs. TAIR10
Match: AT1G31800.1 (cytochrome P450, family 97, subfamily A, polypeptide 3)

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-11
Identity = 29/71 (40.85%), Postives = 48/71 (67.61%), Query Frame = 0


Query: 52  ALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVL-SNYGTHAKGLVSDVSEFVSG 111
           A F+ L+     YG I+RL  GP++F++VSDP+IA H+L  N   ++KG+++++ +FV G
Sbjct: 127 AFFIPLYELFLTYGGIFRLTFGPKSFLIVSDPSIAKHILKDNAKAYSKGILAEILDFVMG 186

Query: 112 SGFAIAEGPLW 122
            G   A+G +W
Sbjct: 187 KGLIPADGEIW 197

HSP 2 Score: 42.0 bits (97), Expect = 8.2e-04
Identity = 19/43 (44.19%), Postives = 26/43 (60.47%), Query Frame = 0


Query: 241 FAHCCNSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           F    N H     WD+AE+F PE + L+GP PNE + +F +LP
Sbjct: 466 FISVWNLHRSPLHWDDAEKFNPERWPLDGPNPNETNQNFSYLP 508

[Close]

BLAST of Carg10449 vs. Swiss-Prot
Match: sp|Q6TBX7|LUT1_ARATH (Carotene epsilon-monooxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP97C1 PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 4.7e-33
Identity = 79/140 (56.43%), Postives = 91/140 (65.00%), Query Frame = 0


Query: 3   PSPYPSIKSPSIAEGNPPTPTTSGAKSGSWVSPDCLTSPTRF------------------ 62
           P P  +    S  E   P   T+ +KS SWVSPD LT+ TR                   
Sbjct: 27  PKPKFTFSIRSSIEKPKPKLETNSSKSQSWVSPDWLTTLTRTLSSGKNDESGIPIANAKL 86

Query: 63  --ITDLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHAKGLVS 122
             + DLLG ALFL L+ WMN+YGPIYRLAAGPRNFV+VSDPAIA HVL NY  +AKGLV+
Sbjct: 87  DDVADLLGGALFLPLYKWMNEYGPIYRLAAGPRNFVIVSDPAIAKHVLRNYPKYAKGLVA 146

HSP 2 Score: 55.1 bits (131), Expect = 1.7e-06
Identity = 20/38 (52.63%), Postives = 30/38 (78.95%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   +VW++AEEF+PE FD++G +PNE +TDF+ +P
Sbjct: 442 NIHRSSEVWEKAEEFLPERFDIDGAIPNETNTDFKFIP 479

[Close]

BLAST of Carg10449 vs. Swiss-Prot
Match: sp|O48921|C97B2_SOYBN (Cytochrome P450 97B2, chloroplastic OS=Glycine max OX=3847 GN=CYP97B2 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.6e-12
Identity = 34/78 (43.59%), Postives = 53/78 (67.95%), Query Frame = 0


Query: 45  ITDLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVL-SNYGTHAKGLVSD 104
           ++DLLG  LF SL++W  ++G +Y+LA GP+ FVVVSDP +A H+L  N  ++ KG+++D
Sbjct: 86  VSDLLGRPLFFSLYDWFLEHGAVYKLAFGPKAFVVVSDPIVARHILRENAFSYDKGVLAD 145

Query: 105 VSEFVSGSGFAIAEGPLW 122
           + E + G G   A+   W
Sbjct: 146 ILEPIMGKGLIPADLDTW 163

[Close]

BLAST of Carg10449 vs. Swiss-Prot
Match: sp|O23365|C97B3_ARATH (Cytochrome P450 97B3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP97B3 PE=1 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 2.7e-12
Identity = 35/78 (44.87%), Postives = 53/78 (67.95%), Query Frame = 0


Query: 45  ITDLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVL-SNYGTHAKGLVSD 104
           ++DL G  LFLSL++W  ++G IY+LA GP+ FVV+SDP IA HVL  N  ++ KG++++
Sbjct: 91  VSDLFGKPLFLSLYDWFLEHGGIYKLAFGPKAFVVISDPIIARHVLRENAFSYDKGVLAE 150

Query: 105 VSEFVSGSGFAIAEGPLW 122
           + E + G G   A+   W
Sbjct: 151 ILEPIMGKGLIPADLDTW 168

[Close]

BLAST of Carg10449 vs. Swiss-Prot
Match: sp|Q43078|C97B1_PEA (Cytochrome P450 97B1, chloroplastic OS=Pisum sativum OX=3888 GN=CYP97B1 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.0e-11
Identity = 33/78 (42.31%), Postives = 51/78 (65.38%), Query Frame = 0


Query: 45  ITDLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVL-SNYGTHAKGLVSD 104
           +TDL    LF SL++W  ++G +Y+LA GP+ FVVVSDP +A H+L  N  ++ KG+++D
Sbjct: 95  VTDLFDRPLFFSLYDWFLEHGSVYKLAFGPKAFVVVSDPIVARHILRENAFSYDKGVLAD 154

Query: 105 VSEFVSGSGFAIAEGPLW 122
           + E + G G   A+   W
Sbjct: 155 ILEPIMGKGLIPADLETW 172

[Close]

BLAST of Carg10449 vs. Swiss-Prot
Match: sp|Q93VK5|LUT5_ARATH (Protein LUTEIN DEFICIENT 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP97A3 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.5e-10
Identity = 29/71 (40.85%), Postives = 48/71 (67.61%), Query Frame = 0


Query: 52  ALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVL-SNYGTHAKGLVSDVSEFVSG 111
           A F+ L+     YG I+RL  GP++F++VSDP+IA H+L  N   ++KG+++++ +FV G
Sbjct: 127 AFFIPLYELFLTYGGIFRLTFGPKSFLIVSDPSIAKHILKDNAKAYSKGILAEILDFVMG 186

Query: 112 SGFAIAEGPLW 122
            G   A+G +W
Sbjct: 187 KGLIPADGEIW 197

[Close]

BLAST of Carg10449 vs. TrEMBL
Match: tr|A0A1S3CIN0|A0A1S3CIN0_CUCME (carotene epsilon-monooxygenase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500849 PE=3 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.9e-39
Identity = 96/146 (65.75%), Postives = 101/146 (69.18%), Query Frame = 0


Query: 3   PSPYPSIKSPSIAEGNPPTP-----TTSGAKSGSWVSPDCLTSPTRFIT----------- 62
           P PYPSIKS     GNP TP      T+  KS SWVSPD LTS TR IT           
Sbjct: 28  PFPYPSIKSSLDERGNPSTPPKLKNPTNAPKSRSWVSPDWLTSLTRSITLGQGDDSGIPI 87

Query: 63  ---------DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHA 122
                    DLLG ALFL LF WMNDYGPIYRLAAGPRNFV+VSDPAIA HVL NYGT+A
Sbjct: 88  ATAKLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVIVSDPAIAKHVLRNYGTYA 147

Query: 123 KGLVSDVSEFVSGSGFAIAEGPLWTV 124
           KGLVS+VSEF+ GSGFAIAEGPLWTV
Sbjct: 148 KGLVSEVSEFLFGSGFAIAEGPLWTV 173

[Close]

BLAST of Carg10449 vs. TrEMBL
Match: tr|A0A2C9VEV7|A0A2C9VEV7_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_08G016300 PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 1.1e-34
Identity = 82/130 (63.08%), Postives = 91/130 (70.00%), Query Frame = 0


Query: 13  SIAEGNPPTPTTSGAKSGSWVSPDCLTSPTRFIT--------------------DLLGPA 72
           S  +   P PT++  KS SWVSPD LTS TR +T                    +LLG A
Sbjct: 34  SSIDNKTPKPTSNATKSSSWVSPDWLTSLTRTLTLGQNDDSGIPIASAKLDDVSELLGGA 93

Query: 73  LFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHAKGLVSDVSEFVSGSG 123
           LFL LF WMN+YGPIYRLAAGPRNFVVVSDPAIA HVL NYG +AKGLVS+VSEF+ GSG
Sbjct: 94  LFLPLFKWMNEYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGKYAKGLVSEVSEFLFGSG 153

HSP 2 Score: 61.2 bits (147), Expect = 4.8e-06
Identity = 24/38 (63.16%), Postives = 30/38 (78.95%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   KVW+ AEEF+PE FDL+GP+PNE +TDFR +P
Sbjct: 439 NIHHSSKVWERAEEFVPERFDLDGPIPNETNTDFRFIP 476

[Close]

BLAST of Carg10449 vs. TrEMBL
Match: tr|A0A2N9FL98|A0A2N9FL98_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS15543 PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 1.4e-34
Identity = 80/123 (65.04%), Postives = 90/123 (73.17%), Query Frame = 0


Query: 20  PTPTTSGAKSGSWVSPDCLTSPTRFIT--------------------DLLGPALFLSLFN 79
           P PT +  K+GSWVSPD LTS +R +T                    DLLG ALFL LFN
Sbjct: 43  PKPTNNSNKTGSWVSPDWLTSLSRSLTINKNDDSGIPIASAKLDDVSDLLGGALFLPLFN 102

Query: 80  WMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHAKGLVSDVSEFVSGSGFAIAEGP 123
           WMN+YGPIYRLAAGPRNFVVVSDPAIA HVL NYG ++KGLV++VSEF+ GSGFAIAEGP
Sbjct: 103 WMNEYGPIYRLAAGPRNFVVVSDPAIAKHVLRNYGKYSKGLVAEVSEFLFGSGFAIAEGP 162

HSP 2 Score: 57.4 bits (137), Expect = 6.9e-05
Identity = 24/38 (63.16%), Postives = 29/38 (76.32%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   +VW+ AEEFIPE FDL+G VPNE +TDFR +P
Sbjct: 385 NIHHSSQVWERAEEFIPERFDLDGQVPNETNTDFRFIP 422

[Close]

BLAST of Carg10449 vs. TrEMBL
Match: tr|A0A067JZ47|A0A067JZ47_JATCU (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_16600 PE=3 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 1.2e-33
Identity = 85/142 (59.86%), Postives = 95/142 (66.90%), Query Frame = 0


Query: 2   IPSPYPSIKSPSIAEGNPPTPTTSGAKSGSWVSPDCLTSPTRFIT--------------- 61
           IP P+  I   S+ E   P P +  AK  SWVSP+ LTS TR +T               
Sbjct: 25  IPKPHFLIIKSSL-ENKTPKPASDTAKPSSWVSPNWLTSLTRTLTLGQNDNSGIPIASAK 84

Query: 62  -----DLLGPALFLSLFNWMNDYGPIYRLAAGPRNFVVVSDPAIANHVLSNYGTHAKGLV 121
                DLLG ALFL LF WMN+YGPIYRLAAGPRNFVVVSDPAIA H+L NYG + KGLV
Sbjct: 85  LEDVSDLLGGALFLPLFKWMNEYGPIYRLAAGPRNFVVVSDPAIAKHLLKNYGKYGKGLV 144

Query: 122 SDVSEFVSGSGFAIAEGPLWTV 124
           S+VSEF+ GSGFAIAEGPLWTV
Sbjct: 145 SEVSEFLFGSGFAIAEGPLWTV 165

HSP 2 Score: 62.8 bits (151), Expect = 1.6e-06
Identity = 26/38 (68.42%), Postives = 30/38 (78.95%), Query Frame = 0


Query: 246 NSHLCFKVWDEAEEFIPELFDLEGPVPNENDTDFRHLP 284
           N H   KVW+ AEEF+PE FDLEGPVPNE +TDFR +P
Sbjct: 440 NIHHSSKVWERAEEFVPERFDLEGPVPNETNTDFRFIP 477

[Close]

BLAST of Carg10449 vs. TrEMBL
Match: tr|A0A0A0KIH3|A0A0A0KIH3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G448700 PE=3 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 1.2e-33
Identity = 120/318 (37.74%), Postives = 154/318 (48.43%), Query Frame = 0


Query: 28  KSGSWVSPDCLTSPTRFIT--------------------DLLGPALFLSLFNWMNDYGPI 87
           KS SWVSPD LTS TR+IT                    DLLG ALFL LF WMNDYGPI
Sbjct: 59  KSRSWVSPDWLTSLTRYITLGQGDDSGIPVATAKLDDVSDLLGGALFLPLFKWMNDYGPI 118

Query: 88  YRLAAGPRNFVVVSDPAIANHVLSNYGTHAKGLVSDVSEFVSGSGFAIAEGPLWTVIFLL 147
           YRLAAGPRNFV+VSDP IA HVL NYGT+AKGLVS+VSEF+ GSGFAIAEGPLWTV    
Sbjct: 119 YRLAAGPRNFVIVSDPTIAKHVLRNYGTYAKGLVSEVSEFLFGSGFAIAEGPLWTVRRRA 178

Query: 148 LLTVLLMSKDKLKIYHREVLKNSLQIAKKLWKLRRNMWMTLIQASFDFCWPVHKSFPFIT 207
           ++   L  K    I  R   K ++++ +KL K           A  +    + + F  +T
Sbjct: 179 VVP-SLHKKYLSVIVDRVFCKCAMRLVEKLEK----------DALNNNSVNMEEKFSQLT 238

Query: 208 NILQNQSRTEYRMNTYILTKIEVKIIYLS---QSFSSILLLPVWKYIRTCSCFVKCFAHC 267
             +   S   Y  ++       +  +Y +       S  +LP WK    C    +     
Sbjct: 239 LDVIGLSVFNYSFDSLSTDSPVIDAVYTALKEAEARSTDILPYWKIKALCKIIPRQIKAE 298

Query: 268 CNSHLCFKVWDEAEEFIPELFDLEGPVPNE----NDTD---FRHLPGAHSHVWCEKLDHR 316
               +  K  +E      E+ + EG   NE    ND D    R L  +   V   +L   
Sbjct: 299 EAVTVIRKTVEELIAKCKEIVEAEGERINEEEYVNDADPSILRFLLASREEVSSVQLRDD 358

The following BLAST results are available for this feature:

BLAST of Carg10449 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143881.1	2.1e-42	40.57	carotene epsilon-monooxygenase, chloroplastic [Momordica charantia]	[more]
XP_022972774.1	1.7e-39	66.22	carotene epsilon-monooxygenase, chloroplastic [Cucurbita maxima]	[more]
XP_008462512.1	2.9e-39	65.75	PREDICTED: carotene epsilon-monooxygenase, chloroplastic [Cucumis melo]	[more]
XP_022962840.1	3.8e-39	65.54	carotene epsilon-monooxygenase, chloroplastic [Cucurbita moschata]	[more]
XP_023518813.1	1.1e-38	67.38	carotene epsilon-monooxygenase, chloroplastic [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg10449 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G53130.1	2.6e-34	56.43	Cytochrome P450 superfamily protein	[more]
AT4G15110.1	1.5e-13	44.87	cytochrome P450, family 97, subfamily B, polypeptide 3	[more]
AT1G31800.1	1.4e-11	40.85	cytochrome P450, family 97, subfamily A, polypeptide 3	[more]

BLAST of Carg10449 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q6TBX7\|LUT1_ARATH	4.7e-33	56.43	Carotene epsilon-monooxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
sp\|O48921\|C97B2_SOYBN	1.6e-12	43.59	Cytochrome P450 97B2, chloroplastic OS=Glycine max OX=3847 GN=CYP97B2 PE=2 SV=1	[more]
sp\|O23365\|C97B3_ARATH	2.7e-12	44.87	Cytochrome P450 97B3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP97B3 P...	[more]
sp\|Q43078\|C97B1_PEA	1.0e-11	42.31	Cytochrome P450 97B1, chloroplastic OS=Pisum sativum OX=3888 GN=CYP97B1 PE=2 SV=...	[more]
sp\|Q93VK5\|LUT5_ARATH	2.5e-10	40.85	Protein LUTEIN DEFICIENT 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP...	[more]

BLAST of Carg10449 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3CIN0\|A0A1S3CIN0_CUCME	1.9e-39	65.75	carotene epsilon-monooxygenase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1035...	[more]
tr\|A0A2C9VEV7\|A0A2C9VEV7_MANES	1.1e-34	63.08	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_08G016300 PE=3 SV=...	[more]
tr\|A0A2N9FL98\|A0A2N9FL98_FAGSY	1.4e-34	65.04	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS15543 PE=3 SV=1	[more]
tr\|A0A067JZ47\|A0A067JZ47_JATCU	1.2e-33	59.86	Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_16600 PE=3 SV=1	[more]
tr\|A0A0A0KIH3\|A0A0A0KIH3_CUCSA	1.2e-33	37.74	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G448700 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506	iron ion binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450
IPR036396	Cyt_P450_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg10449-RA	Carg10449-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	G3DSA:1.10.630.10		coord: 41..233 e-value: 1.8E-8 score: 35.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	SSF48264	Cytochrome P450	coord: 49..234
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 56..143 e-value: 1.4E-6 score: 27.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..33
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 14..33
None	No IPR available	PANTHER	PTHR24291:SF52	CAROTENE EPSILON-MONOOXYGENASE, CHLOROPLASTIC	coord: 45..123
None	No IPR available	PANTHER	PTHR24291	FAMILY NOT NAMED	coord: 45..123
None	No IPR available	PANTHER	PTHR24291:SF52	CAROTENE EPSILON-MONOOXYGENASE, CHLOROPLASTIC	coord: 245..284
None	No IPR available	PANTHER	PTHR24291	FAMILY NOT NAMED	coord: 245..284

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg10449	Cla018347	Watermelon (97103) v1	carwmB0227
Carg10449	Csa6G448700	Cucumber (Chinese Long) v2	carcuB0267
Carg10449	MELO3C024495	Melon (DHL92) v3.5.1	carmeB0242
Carg10449	ClCG04G009570	Watermelon (Charleston Gray)	carwcgB0232
Carg10449	CSPI06G24970	Wild cucumber (PI 183967)	carcpiB0271
Carg10449	Cucsa.124950	Cucumber (Gy14) v1	carcgyB0281
Carg10449	CmaCh03G011890	Cucurbita maxima (Rimu)	carcmaB0333
Carg10449	CmaCh07G008850	Cucurbita maxima (Rimu)	carcmaB0335
Carg10449	CmoCh07G009170	Cucurbita moschata (Rifu)	carcmoB0338
Carg10449	Lsi01G012770	Bottle gourd (USVL1VR-Ls)	carlsiB214
Carg10449	Cp4.1LG19g03680	Cucurbita pepo (Zucchini)	carcpeB0338
Carg10449	CsGy6G024390	Cucumber (Gy14) v2	carcgybB0255
Carg10449	MELO3C024495.2	Melon (DHL92) v3.6.1	carmedB0250
Carg10449	CsaV3_6G040180	Cucumber (Chinese Long) v3	carcucB0270
Carg10449	Cla97C04G076340	Watermelon (97103) v2	carwmbB0244
Carg10449	Bhi03G001172	Wax gourd	carwgoB0306

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg10449	Carg25570	Silver-seed gourd	carcarB278

The following block(s) are covering this gene:


Gene	Organism	Block
Carg10449	Silver-seed gourd	carcarB276
Carg10449	Silver-seed gourd	carcarB279
Carg10449	Cucurbita maxima (Rimu)	carcmaB0331
Carg10449	Cucurbita moschata (Rifu)	carcmoB0334
Carg10449	Cucurbita moschata (Rifu)	carcmoB0336
Carg10449	Cucurbita pepo (Zucchini)	carcpeB0337
Carg10449	Cucurbita pepo (Zucchini)	carcpeB0339
Carg10449	Cucurbita pepo (Zucchini)	carcpeB0340
Carg10449	Wax gourd	carwgoB0302