Carg10436 (gene) Silver-seed gourd

NameCarg10436
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionHistidine--trna ligase
LocationCucurbita_argyrosperma_scaffold_098 : 139511 .. 145196 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCGAAGCCTTCATCGCTCCTTATCTTCCAACCTCGCCTCAATCTTCGTTTGAAGCCCTTCATTTTCTCTTCTTCGTCACTCGCTTTCAGTATCATTCTTAGAATCCCCTTTCCGAAGGCCTCATTCTTCGGTAACCGCAAATTTCATTCTTCTCTCAGCGCCGTCTCTTCCGCCCAGTCTACCGGTGACGGCGGAAATGGTGGCGGCAGGTCGGGGGCGCTATCTCCGACGCTGGTCACTGAAGAATTGCAGAAGATCGATGTTAACCTCCAAGGAACCAAGGGATTTATTTCCGCCGGGACATATAACTGATAGCGGACTTCCGCAAACTAGATTCCTAATTCAATAATTTCAGTGTAGAGGTTTCGTAATCCGTCGCAAAATTGTATGTAGTTTTGCCTTTATTTTGTACGGTTTTTGTGTACTAATGTCTTTAAAGTTTTTGTTGAGGTTCTTTCACATCAAAGTCTCATGAACTAGGACTTCAATCGATTCATACTGGAATTTCGAGTCCAGCGTGAGTGTTGACTTTCCCCTTGAGTCCAGAGTGTGCGGTTCAGTTCCTTACATTATGCGATCAATAAATAAGAACCAGGTTGGAACGAAATACGTAAGGGTATAATAATTGAGTCAGTAATGTTTCCGTAATTTACCTTCCTTATTGTAAACCAAAAATGTATTCGAAGTATGTTCTGTTAATGCATACTGTGGAATTTTTGCTGTCAAGCCCTTAACTGGACTATTTCTTGGACGACTCATATAGTCCATTAGGAATTGTAGCACCATACAAACTTCTTTGATGCCAATGAGAAGGAAAAGAATGGCGTGTGTAGTTATCCTAGTAGATCTATACATACCCCGATAATTTAGCACTTAAATTTGGTCCTTTTAACGGTATTCCATTCAGCGACGTTTCGCCCAAATTCTTCTTTTGAGCTTAGTATTGCTAGTGCACCCATCGTACGCGCTTACTTTCCCCGTGCCCCCAACCGCTAGTTTAATGTCGGACAAACCACAAGTGCATGCGCTCTCTCTAACAGACGATTAGCATAATTGTATATTGCATAACTTATTTATTCGTAAATAACAATCTATCTTCTGAATGTTGGAAATCTGAGTATCTTAGTGCTAGATACGCTGTCGATTTCGTGCTCTTATTATGTTATAGCTCAATTGGTATGTTTGAGAAATCTCCGAGGGCATAAATATGGTAGAGTATTCTTTGACACCATAGTGCTCACTCCTTCTTCTGTCCAGGACGTAGTGATACTATTGAAAGGCACCGTTAACTTTGACATCTCATTTTTTGACGTGCATATGTGGTTTTTCATTTATAACTTTGAATAGTAGTGTTGCTTGTACTTATCTATACTTTTTCGGATTTCCCTGTTAATATACAGCAAAAGTTGATGATGAGGATAGCCATCGCCACGCGTGAGTGTGAAAAATATTCACAAAGTATTGTCGTTATCATAGATTTGTTACAATTTGAATCTTATCATTCTTTATGTATAATCATTCGTGCAGATGTCAGAATTATAGCATCACCACCTTTTTTTCTGTCATCACGACTAGAAGAATCTGTTTCCCTTCCCACTGATGAATTATGTTTTTGCGTACTAGGGTCACAAGCGAAGCGTGACACATGTGCGGCCGGATTCCGGAGTTTATGAAAGCATGAGGAGCGCCTAAGAGACTAGAGGGGATCACGCCGTGGCCATGTCTGGCATCACTACCAATGGATATGGATCATCTAATTGTGAGATACCCCTCTAGAGCGTTTTTGTTATCTTATCGTTAACTGTCTAGAGACGTTAATGCTAGTGGAACATACCTTATCTAATGGGGGAATATTGGATTGTGCATGATGTTTTAGATCATTTATATGACAAATATGGCTTGTTTAAGTGAGTGATAAAAGAGATAACACTCATCAAACAATCTATTGAAACTTTCTATCCCTTTCATTAACACTAGGATGCGTGTCCCTTCATTTCAATGCCATTTACAATAGAGTTCTGCACGCTGTGAGCTATGCATGGATCTTCATATATTCTTTTAATTGTGTAAGCTTAAAAAAATATAAAATATTGCGAAAAAATCAATATACATTTTAAAAATCCCTAATATTTGATGATTGTTAAACAAAATAATTGTGTTTATTCGCATCGCTTATTGGACGACCTATTCACCACGTTTATACAGTCCCAGTCATCAGGTTGAAATTTAAAGTTGTGCGGAGTTATCAAAAGTTCAGACTCATCACCGTACTAAAATACAGTTTTTTCTTTTACATTTCTTTAGTATATGAGAAAATGAAAGTTTATATGCAAGGGGTGAATAAATGATTTAGGCAGCTTTGAGAGCTTTTGTTAGCATTTTTTATGTTCTTCCATATTTATTGCTTCCATGTCTGTAGTTAGATAACTAGCTCATGCGTTCTCTAAAGATGCATGATGCAATAAATGGTATTTTTGTTTTCTGGTGCAGCAAAGAAGATAATAGCAAGCACTTAAAAAGTCGATTTCTGTCATTTTTTCTATGGATATTTCTGTTGGTAAATCTTTAGTCTTCTCAAGTGTTTGTGGGTTCTGAGCCTGTAATTTAGTTTATCTCGTCCTTTGTTAGTTGCTGCTTTTCATTTCTTTACCTTAGGAAAAAAAAATGTGAACCCATCTAGCCATGTTGACATGTTCTAGTTGTAAACTCTTTGGTACCTGCCTTGTTTGAACAGGGTACCTTTTTATATATATACAAATATGCATGTGTGCACATTTTATTTAAGCTCATATATATAAATATATATTTTGTGTAGGCCGAAGCAGAGCTTCTATCTTCTATTGTTGCCTTTTTCAAACGAATCGGACTTACAGCAGCAGATGTTGGATTTAAGGTTTCCAGTCGAAAGGTATTTTTTTTCTGCATCTTTTGTGATTATTCCAATAAATTTAGTTATTGCTTTATTAATTATTGAACATTCAATCATGAATCGAAGACTTTAATTGTTGTAGTTTCTGTAAATGCCTTATGTTGTAAGTTTGATGGTAGGTTTTGCAAGAAGTATTAAAATGCTACGGAATACCTGAAAATATGTTTGGCAAGGTTTGCATCATCATAGACAAGGTTGGTTATCTATGCCTTCAAGTTCCATAAATATTTACATTTTTGTGCACGTTCTTGACGCACGTCTCGATGTAATAATTTCTTCTGTCTCTGTTTCCTTCTGGCTATTAATATTAACAACAGATAGAAAAAATTCCATTGGATGAGATGAAGAAGGAACTGAAGGTTGTTGGTGTATCAGAGGATGCTATCGAGGAAATGTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGTTAGAAGGTTATTTTCTGCAAATGGTTAAGTCTTTAGCAAATTAGTTGAAATGCATGTGATAATTATGAAAAATGAAAATCTGATGAAGACATTGAAATTAATTGAAGCGTGATTGTTATCCTTGCTTAGAGATACTTGGAGGTGGTGGTGAAGCCATGTCTGATCTGAAGCAGCTATTTTCTCTTGCTGAGAAGTTTGGTTATTCTGATTGGATCCAATTCGACGCGTCAATTGTCCGCGGCCTTGCTTATTACACTGGTATTGTTTTTGAGGTTGGTATTTGATATTCACTCCTTGTTGATTTGACTTGTCTTCATGCTGTAGAATAGGTCAAACATATATTATATGTCATTAACTGACCGAATGCCACGTTTGACACGTTAAACTACAGTGAGCTTCTAGATTCTCATGTAAATGGAATAATGTCGGCAAAGCTTCACACTAAATGGCTACAATCCCCATGTATAGTCTAATATTAAAAATTATATGAAATTTTGAATATCAAATAATTTCATATATCTTGGTGTATTTGATCATAAATATAATATCCAGCTAAGGAATGGATTGGTAACAGTATGTTTTCTTTGTATATCGTCAAACATAATTGCTGCAACTGAACAATATGCGTGTTTATTCTTTTTCCTCGTTTCTTTAGTCAATATTTCTTCATGAACTCTGTCTTCATATAAATAAATGTTACTTGGCTATATTTATCTCTTGCTTATTGTTTTATGGAAATAATTGGTAAACTGATCAAAGTCGTATTTGTTCTCTCTTGTCATTGTATCAGTTTAATTGAAGGAAAGACAATGCCATGAAATTGCTCTTTCGGTTCCTTTTAATCATGAAGATGCTTTTTCAGGGGTTTGACAGAGGGGGAAAGCTGCGAGCCATATGTGGTGGAGGTCGGTATGATCGTTTGCTTTCTACCTTTGGTGGTGACGATCTTCCTGCTTGTGGGTTCGGGTTTGGTGATGCTGTTATAGTAGAGGTACGTTTTAAGCTGAACTCTGCATCCTTACACAATCATAAAAAAATTCTATGAATTCTATGCTTAATGTTAATCATAGAACGTAACGTGGATGAACTTTTACTGGGGATATTATTAACGTCATGCTTTTTGATCAACTGTGTTCGACTGTTCATTATTTAATAAGCACCTTGAATTGGCTCATAGTTAAGAATCTTTTGGTTCAAACCATGTAGGCCGTTTATCTCATGTATTATTAAGTATCCTACCAATTTCTTGATATCAAATCTTTTAGTATTAAGTTGTTGTTTGGTAGATGTAGTTGAGGAGTGTTAAACTAACTCATACTCAGGGAAAACTTTCAATTTTGTTTTATTGAGTATTGAACAAGGGGTGAAATCAAGACCTTCTCAAAACTAAATTAGAACATTGTTCATCACTAGCCAAAAAAACCACACAATCTCAGCTAACTAAGCATTCAAATTCAAGTCATAGAAATGCTCAAATTGATCTATGAAAAATACAAAGTAGGTACAAAAAATTAAAAAAACAAAATCCTAAACTGAACAGTAAAATGCTTAATCCTAGGTAGAAGACATCCATATGATCAAAATAACCCACTTTTACCGAAAACTGTCATACCCCATCACAAGAACGCACATAGCTGACTGCAAATCATGTTGAAATAAAAAAAACCAATGAAAACAATGGAACTGAGATGGTTCCCCACCCATTGGAAGTACACCCATTAGTTCAAGTCACTAATATCGATTTCATGGGTCATTTCTATGATCAACATTATGTTGAACCAAACCACTTAAATCAACATGCCTTTTGAGGAAAGAACCAAACCACATAACTCTCTACGTGGTAGAAGTTCTTTGATGAATACACTTTTTTTTATCATACTTATCGATAGCTCGGATTTGAGATATTTGTTTTGTGCCTGGACGTGGGGTTTGGTTGTAGTTGCTTAAGGAAAAGAAGTTGCTGCCAGAACTGAATGTTGAAGTAGAGAACATAGTTTGTGCTCTGGATCCTGATCTCCAAGGCGCAGCTTCAAGAGTTGCTACCATTCTTAGGGAAAAAGGCCAAACTGTTGATTTGGTATTGGAGAATAAACCTCTCAAATGGTATCATTCACAAAACCCGATTAACTTCATCAAAATTATCACAATTTCTGATCCACCCTGATTTGCTTCATCTTTTGCAGGGTGTTTAAACGAGCGGCACGGATAAACGCTCAGCGGTTGATACTAGTCGGAAGTTCAGAATGGCAACGAGGCATGGTCGGAGTAAAAGATCTTTCCTCAGGTGAGCAACGCGAGGTCAAACTTGAGGAACTCAACTAA

mRNA sequence

ATGGCTGCGAAGCCTTCATCGCTCCTTATCTTCCAACCTCGCCTCAATCTTCGTTTGAAGCCCTTCATTTTCTCTTCTTCGTCACTCGCTTTCAGTATCATTCTTAGAATCCCCTTTCCGAAGGCCTCATTCTTCGGTAACCGCAAATTTCATTCTTCTCTCAGCGCCGTCTCTTCCGCCCAGTCTACCGGTGACGGCGGAAATGGTGGCGGCAGGTCGGGGGCGCTATCTCCGACGCTGGTCACTGAAGAATTGCAGAAGATCGATGGTCACAAGCGAAGCGTGACACATGTGCGGCCGGATTCCGGAGCCGAAGCAGAGCTTCTATCTTCTATTGTTGCCTTTTTCAAACGAATCGGACTTACAGCAGCAGATGTTGGATTTAAGGTTTCCAGTCGAAAGGTTTTGCAAGAAGTATTAAAATGCTACGGAATACCTGAAAATATGTTTGGCAAGGTTTGCATCATCATAGACAAGATAGAAAAAATTCCATTGGATGAGATGAAGAAGGAACTGAAGGTTGTTGGTGTATCAGAGGATGCTATCGAGGAAATGTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGTTAGAAGAGATACTTGGAGGTGGTGGTGAAGCCATGTCTGATCTGAAGCAGCTATTTTCTCTTGCTGAGAAGTTTGGTTATTCTGATTGGATCCAATTCGACGCGTCAATTGTCCGCGGCCTTGCTTATTACACTGGTATTGTTTTTGAGGGGTTTGACAGAGGGGGAAAGCTGCGAGCCATATGTGGTGGAGGTCGGTATGATCGTTTGCTTTCTACCTTTGGTGGTGACGATCTTCCTGCTTGTGGGTTCGGGTTTGGTGATGCTGTTATAGTAGAGTTGCTTAAGGAAAAGAAGTTGCTGCCAGAACTGAATGTTGAAGTAGAGAACATAGTTTGTGCTCTGGATCCTGATCTCCAAGGCGCAGCTTCAAGAGTTGCTACCATTCTTAGGGAAAAAGGCCAAACTGTTGATTTGGTATTGGAGAATAAACCTCTCAAATGGGTGTTTAAACGAGCGGCACGGATAAACGCTCAGCGGTTGATACTAGTCGGAAGTTCAGAATGGCAACGAGGCATGGTCGGAGTAAAAGATCTTTCCTCAGGTGAGCAACGCGAGGTCAAACTTGAGGAACTCAACTAA

Coding sequence (CDS)

ATGGCTGCGAAGCCTTCATCGCTCCTTATCTTCCAACCTCGCCTCAATCTTCGTTTGAAGCCCTTCATTTTCTCTTCTTCGTCACTCGCTTTCAGTATCATTCTTAGAATCCCCTTTCCGAAGGCCTCATTCTTCGGTAACCGCAAATTTCATTCTTCTCTCAGCGCCGTCTCTTCCGCCCAGTCTACCGGTGACGGCGGAAATGGTGGCGGCAGGTCGGGGGCGCTATCTCCGACGCTGGTCACTGAAGAATTGCAGAAGATCGATGGTCACAAGCGAAGCGTGACACATGTGCGGCCGGATTCCGGAGCCGAAGCAGAGCTTCTATCTTCTATTGTTGCCTTTTTCAAACGAATCGGACTTACAGCAGCAGATGTTGGATTTAAGGTTTCCAGTCGAAAGGTTTTGCAAGAAGTATTAAAATGCTACGGAATACCTGAAAATATGTTTGGCAAGGTTTGCATCATCATAGACAAGATAGAAAAAATTCCATTGGATGAGATGAAGAAGGAACTGAAGGTTGTTGGTGTATCAGAGGATGCTATCGAGGAAATGTTGCAAGTTCTTTCAGTAAAGTCTTTGACAAAGTTAGAAGAGATACTTGGAGGTGGTGGTGAAGCCATGTCTGATCTGAAGCAGCTATTTTCTCTTGCTGAGAAGTTTGGTTATTCTGATTGGATCCAATTCGACGCGTCAATTGTCCGCGGCCTTGCTTATTACACTGGTATTGTTTTTGAGGGGTTTGACAGAGGGGGAAAGCTGCGAGCCATATGTGGTGGAGGTCGGTATGATCGTTTGCTTTCTACCTTTGGTGGTGACGATCTTCCTGCTTGTGGGTTCGGGTTTGGTGATGCTGTTATAGTAGAGTTGCTTAAGGAAAAGAAGTTGCTGCCAGAACTGAATGTTGAAGTAGAGAACATAGTTTGTGCTCTGGATCCTGATCTCCAAGGCGCAGCTTCAAGAGTTGCTACCATTCTTAGGGAAAAAGGCCAAACTGTTGATTTGGTATTGGAGAATAAACCTCTCAAATGGGTGTTTAAACGAGCGGCACGGATAAACGCTCAGCGGTTGATACTAGTCGGAAGTTCAGAATGGCAACGAGGCATGGTCGGAGTAAAAGATCTTTCCTCAGGTGAGCAACGCGAGGTCAAACTTGAGGAACTCAACTAA

Protein sequence

MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSSAQSTGDGGNGGGRSGALSPTLVTEELQKIDGHKRSVTHVRPDSGAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNVEVENIVCALDPDLQGAASRVATILREKGQTVDLVLENKPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKDLSSGEQREVKLEELN
BLAST of Carg10436 vs. NCBI nr
Match: XP_023544233.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 630.6 bits (1625), Expect = 3.6e-177
Identity = 376/507 (74.16%), Postives = 381/507 (75.15%), Query Frame = 0

Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVXXX 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAV XX
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSXX 60

Query: 61  XXXXXXXXXXXXXXALSPTLVTEELQKID------------------------------- 120
           XXXXXXXXXXXXXXALSPTLV EELQKID                               
Sbjct: 61  XXXXXXXXXXXXXXALSPTLVAEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 -----------------------------------GHKR--------------------- 180
                                              G++R                     
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 -----------------SVTHVR--------------PDSGAEAELLSSIVAFFKRIGLT 240
                             +T  R              P+  AEAELLSSIVAFFKRIGLT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 390
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 420

BLAST of Carg10436 vs. NCBI nr
Match: XP_022925718.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita moschata])

HSP 1 Score: 629.8 bits (1623), Expect = 6.2e-177
Identity = 377/507 (74.36%), Postives = 382/507 (75.35%), Query Frame = 0

Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVXXX 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVXXX
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVXXX 60

Query: 61  XXXXXXXXXXXXXXALSPTLVTEELQKID------------------------------- 120
           XXXXXXXXXXXXXXALSPTLVTEELQKID                               
Sbjct: 61  XXXXXXXXXXXXXXALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 -----------------------------------GHKR--------------------- 180
                                              G++R                     
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 -----------------SVTHVR--------------PDSGAEAELLSSIVAFFKRIGLT 240
                             +T  R              P+  AEAELLSSIVAFFKRIGLT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 390
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 420

BLAST of Carg10436 vs. NCBI nr
Match: XP_022978407.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima])

HSP 1 Score: 627.5 bits (1617), Expect = 3.1e-176
Identity = 358/507 (70.61%), Postives = 365/507 (71.99%), Query Frame = 0

Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVXXX 60
           MAAKPSSLLIFQPRLNLRLKPFIFSS+SLAFSIILRIPFPK SFFGNRKFHSSLSAV   
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSTSLAFSIILRIPFPKPSFFGNRKFHSSLSAVSSA 60

Query: 61  XXXXXXXXXXXXXXALSPTLVTEELQKID------------------------------- 120
                         ALSPTLVTEELQKID                               
Sbjct: 61  QSIGDGGNGGGRSGALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 -----------------------------------GHKR--------------------- 180
                                              G++R                     
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIREQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 -----------------SVTHVR--------------PDSGAEAELLSSIVAFFKRIGLT 240
                             +T  R              P+  AEAELLSSIVAFFKRIGLT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 390
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPEL+V
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELSV 420

BLAST of Carg10436 vs. NCBI nr
Match: XP_023544232.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 624.8 bits (1610), Expect = 2.0e-175
Identity = 376/511 (73.58%), Postives = 381/511 (74.56%), Query Frame = 0

Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVXXX 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAV XX
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVSXX 60

Query: 61  XXXXXXXXXXXXXXALSPTLVTEELQKID------------------------------- 120
           XXXXXXXXXXXXXXALSPTLV EELQKID                               
Sbjct: 61  XXXXXXXXXXXXXXALSPTLVAEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 -----------------------------------GHKR--------------------- 180
                                              G++R                     
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 -----------------SVTHVR--------------PDSGAEAELLSSIVAFFKRIGLT 240
                             +T  R              P+  AEAELLSSIVAFFKRIGLT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFE----GFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP 390
           IVFE    GFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP
Sbjct: 361 IVFEMLFQGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP 420

BLAST of Carg10436 vs. NCBI nr
Match: XP_022925717.1 (histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 624.0 bits (1608), Expect = 3.4e-175
Identity = 377/511 (73.78%), Postives = 382/511 (74.76%), Query Frame = 0

Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVXXX 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVXXX
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVXXX 60

Query: 61  XXXXXXXXXXXXXXALSPTLVTEELQKID------------------------------- 120
           XXXXXXXXXXXXXXALSPTLVTEELQKID                               
Sbjct: 61  XXXXXXXXXXXXXXALSPTLVTEELQKIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 -----------------------------------GHKR--------------------- 180
                                              G++R                     
Sbjct: 121 YGFEEVDFPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 -----------------SVTHVR--------------PDSGAEAELLSSIVAFFKRIGLT 240
                             +T  R              P+  AEAELLSSIVAFFKRIGLT
Sbjct: 181 LSLPLKWFAIGQCWRYERMTRGRRREHYQWNMDIIGVPEVTAEAELLSSIVAFFKRIGLT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFE----GFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP 390
           IVFE    GFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP
Sbjct: 361 IVFEMLFQGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLP 420

BLAST of Carg10436 vs. TAIR10
Match: AT3G46100.1 (Histidyl-tRNA synthetase 1)

HSP 1 Score: 443.7 bits (1140), Expect = 1.1e-124
Identity = 222/289 (76.82%), Postives = 251/289 (86.85%), Query Frame = 0

Query: 100 PDSGAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDK 159
           P   AEAEL+SSIV FFKRIG+TA+DVGFKVSSRKVLQE+LK YG+PE++FG+VCIIIDK
Sbjct: 197 PQVTAEAELISSIVTFFKRIGITASDVGFKVSSRKVLQELLKKYGVPEDLFGRVCIIIDK 256

Query: 160 IEKIPLDEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAE 219
           IEKIP+DE+KKEL   G+SEDAIE++L              +GG GEA++DLKQLFSLAE
Sbjct: 257 IEKIPIDEIKKELGFTGISEDAIEQLLXXXXXXXXXXXXXXIGGAGEAIADLKQLFSLAE 316

Query: 220 KFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACG 279
           KFGYS+WIQFDAS+VRGLAYYTGIVFEGFDR GKLRAICGGGRYDRLLST+GGDD PACG
Sbjct: 317 KFGYSEWIQFDASVVRGLAYYTGIVFEGFDRKGKLRAICGGGRYDRLLSTYGGDDFPACG 376

Query: 280 FGFGDAVIVELLKEKKLLPELNVEVENIVCALDPDLQGAASRVATILREKGQTVDLVLEN 339
           FGFGDAVIVELLKEK LLPEL  EVENIVCALD DLQGAA+ VAT LR+KGQTVDLVLE+
Sbjct: 377 FGFGDAVIVELLKEKDLLPELGQEVENIVCALDKDLQGAAATVATALRDKGQTVDLVLES 436

Query: 340 KPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKDLSSGEQREVKLEEL 389
           KPLKWVFKRAAR+NA+RL+LVG +EW+ G V VK LSSGEQ +VKL +L
Sbjct: 437 KPLKWVFKRAARVNARRLVLVGKTEWEDGSVSVKVLSSGEQFQVKLSDL 485

BLAST of Carg10436 vs. TAIR10
Match: AT3G02760.1 (Class II aaRS and biotin synthetases superfamily protein)

HSP 1 Score: 104.4 bits (259), Expect = 1.6e-22
Identity = 85/278 (30.58%), Postives = 134/278 (48.20%), Query Frame = 0

Query: 121 LTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKEL-KVVGVSE 180
           L   D   K++ RK+L  +L+  G+P   F  +C  IDK++K   +++KKE+ +  G+S 
Sbjct: 590 LEIGDYEVKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSS 649

Query: 181 DAIEEMLQVLSVKS-----LTKL----EEILG--GGGEAMSDLKQLFSLAEKFGYSDWIQ 240
           +  + +   +  K      L+KL     E L      EA+ +L  +F   ++   S+ I 
Sbjct: 650 EIADRIGNFVKEKGAPLELLSKLRQEGSEFLDNQSSREALDELSIMFEALKRSKCSERIV 709

Query: 241 FDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFG-DAV- 300
           FD S+ RGL YYTG++FE    G ++ +I  GGRYD L+  FG   +PA G   G + V 
Sbjct: 710 FDLSLARGLDYYTGVIFEAVCIGAEVGSIGAGGRYDNLIGMFGTKQVPAVGMSLGIERVF 769

Query: 301 -IVELLKEKKLLPELNVEVENIVCAL-DPDLQGAASRVATILREKGQTVDLVLENKPLKW 360
            I+E L EK+       E + +V  + D  L  AA  V+ +   K     LV + K    
Sbjct: 770 NIMEELNEKQKQVIRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAEYLVSKRKEKH- 829

Query: 361 VFKRAARINAQRLILVGSSEWQRGMVGVKDLSSGEQRE 383
            F RA       +++VG  E     V +K L  G + +
Sbjct: 830 -FNRAKESGIPWMVMVGEKELSGSFVTLKKLEKGSEEK 865

BLAST of Carg10436 vs. TAIR10
Match: AT5G03406.1 (Class II aaRS and biotin synthetases superfamily protein)

HSP 1 Score: 97.4 bits (241), Expect = 2.0e-20
Identity = 45/67 (67.16%), Postives = 58/67 (86.57%), Query Frame = 0

Query: 100 PDSGAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDK 159
           P+  AEAEL+SSI+ FFKRIG+TA+DVGFKVSSRKVLQE LK YG+PE++FG+VC IIDK
Sbjct: 197 PEVTAEAELISSIMTFFKRIGITASDVGFKVSSRKVLQEFLKKYGVPEDLFGRVCFIIDK 256

Query: 160 IEKIPLD 167
           +  + ++
Sbjct: 257 VSNLNIN 263

BLAST of Carg10436 vs. Swiss-Prot
Match: sp|O82413|SYHM_ARATH (Histidine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g46100 PE=2 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.1e-123
Identity = 222/289 (76.82%), Postives = 251/289 (86.85%), Query Frame = 0

Query: 100 PDSGAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDK 159
           P   AEAEL+SSIV FFKRIG+TA+DVGFKVSSRKVLQE+LK YG+PE++FG+VCIIIDK
Sbjct: 197 PQVTAEAELISSIVTFFKRIGITASDVGFKVSSRKVLQELLKKYGVPEDLFGRVCIIIDK 256

Query: 160 IEKIPLDEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAE 219
           IEKIP+DE+KKEL   G+SEDAIE++L              +GG GEA++DLKQLFSLAE
Sbjct: 257 IEKIPIDEIKKELGFTGISEDAIEQLLXXXXXXXXXXXXXXIGGAGEAIADLKQLFSLAE 316

Query: 220 KFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACG 279
           KFGYS+WIQFDAS+VRGLAYYTGIVFEGFDR GKLRAICGGGRYDRLLST+GGDD PACG
Sbjct: 317 KFGYSEWIQFDASVVRGLAYYTGIVFEGFDRKGKLRAICGGGRYDRLLSTYGGDDFPACG 376

Query: 280 FGFGDAVIVELLKEKKLLPELNVEVENIVCALDPDLQGAASRVATILREKGQTVDLVLEN 339
           FGFGDAVIVELLKEK LLPEL  EVENIVCALD DLQGAA+ VAT LR+KGQTVDLVLE+
Sbjct: 377 FGFGDAVIVELLKEKDLLPELGQEVENIVCALDKDLQGAAATVATALRDKGQTVDLVLES 436

Query: 340 KPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKDLSSGEQREVKLEEL 389
           KPLKWVFKRAAR+NA+RL+LVG +EW+ G V VK LSSGEQ +VKL +L
Sbjct: 437 KPLKWVFKRAARVNARRLVLVGKTEWEDGSVSVKVLSSGEQFQVKLSDL 485

BLAST of Carg10436 vs. Swiss-Prot
Match: sp|B1ZUW6|SYH_OPITP (Histidine--tRNA ligase OS=Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1) OX=452637 GN=hisS PE=3 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.7e-37
Identity = 106/300 (35.33%), Postives = 158/300 (52.67%), Query Frame = 0

Query: 100 PDSGAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDK 159
           P   AE EL++ +    K  GLT  D   ++S R +    L+  G+ E     V   +DK
Sbjct: 132 PGPEAEIELIALLTQCLKAFGLTEQDFYIRLSDRNLWFFYLEALGLDEPRIRAVLSAVDK 191

Query: 160 IEKIPLDEMKKELKVVGVSEDAIEE-MLQVLSVKSLTKLEEILGG-GGEAMS----DLKQ 219
            EK+  D  K   +  G  + A+++ +L+ L +K+L  LE+ L   GGE ++    D ++
Sbjct: 192 FEKVGDDAFKPYAEQFGPLDLALKQRVLEFLQIKTLASLEQTLAPLGGEKLAARLGDWRK 251

Query: 220 LFSLAEKFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGD 279
           L       G S +I+ D  +VRGLAYYTG VFE FDR G+LRAI GGGRY+ L+   GG 
Sbjct: 252 LLDGLAAMGLSPFIEVDLGVVRGLAYYTGFVFEAFDRKGELRAIAGGGRYNDLVKKLGGP 311

Query: 280 DLPACGFGFGDAVIVELLKEKKLLPELNVEVENIVCALDPDLQGAASRVATI-----LRE 339
           DLPA GF  GD  +  LL  + L+P   V+  ++ C +     GAA R A       LR 
Sbjct: 312 DLPAVGFAIGDVTLGLLLDARGLMPAF-VQASDVYCVIG----GAAERQAAFADVNALRA 371

Query: 340 KGQTVDLVLENKPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKDLSSGEQREVKLEEL 389
            G  VD  L++ P    FK AA   A+  ++ G  E  + +V ++DL++  + +V  E++
Sbjct: 372 AGFKVDYPLKDVPFGKQFKLAADSGAKLALIYGPDELAKNVVKLRDLTTRTETDVPREQV 426

BLAST of Carg10436 vs. Swiss-Prot
Match: sp|Q8U431|SYH_PYRFU (Histidine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=hisS PE=3 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.5e-36
Identity = 96/295 (32.54%), Postives = 164/295 (55.59%), Query Frame = 0

Query: 104 AEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKI 163
           A+AE+++  V  +   GL    V   +  R +L E  K  G+ +++   +  IIDK +K+
Sbjct: 134 ADAEVIALFVESYLATGLREFTV--NIGDRILLDEFAKMLGVKDDI--GLMRIIDKKDKL 193

Query: 164 PLDEMKKELKVVGVSEDAIEEMLQVLSVK----SLTKLEEILGGGGEAMSDLKQLFS--- 223
           P ++    LK  G+ ++ IE++L+++++K     +  L E L     A +++ +L++   
Sbjct: 194 PQEDFINALKEFGLDKNGIEKVLELINIKGKPDEVLPLAEELFTSEVAKNEINRLYALID 253

Query: 224 LAEKFGYSDWIQFDASIVRGLAYYTGIVFEGF---DRGGKLRAICGGGRYDRLLSTFGGD 283
           L E +   DWI+ D  I RG  YYT IVFE     D G  + +I GGGRYD L+  FGG 
Sbjct: 254 LLEAYEVKDWIRIDLGIARGFDYYTSIVFEAIAPNDLG--IGSIGGGGRYDNLIEVFGGK 313

Query: 284 DLPACGFGFGDAVIVELLKEKKLLPELNVEVENIVCALDPDLQGAASRVATILREKGQTV 343
             PA GF  G   ++ +L+ K L+PE+ +  +  V  +   ++  A R+AT LR+ G   
Sbjct: 314 PTPATGFAIGIERLIPILEAKGLIPEVQIAPDVYVVPIGERMKKVAIRIATSLRKSGIKT 373

Query: 344 DLVLENKPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKDLSSGEQREVKLEEL 389
           ++ L  + LK     A ++  ++ I+VG  + +RG+V V+D+ SGEQ EVK++++
Sbjct: 374 EIELMGRKLKKAMDYANKVGVKKTIIVGEKDLERGVVTVRDMESGEQNEVKIDDI 422

BLAST of Carg10436 vs. Swiss-Prot
Match: sp|Q9HLX5|SYH_THEAC (Histidine--tRNA ligase OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) OX=273075 GN=hisS PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.7e-35
Identity = 92/287 (32.06%), Postives = 158/287 (55.05%), Query Frame = 0

Query: 104 AEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKI 163
           A+AE+++   +   R+GL       +++SRK+++E++   G+  +    V  IID+  KI
Sbjct: 137 ADAEVIALASSILDRLGLQDI-YEIRINSRKIMEEIIG--GMTSSDPFSVFSIIDRYHKI 196

Query: 164 PLDEMKKELKVVGVSEDAIEEMLQVLS-VKSLTKLEEILGGGGEAMSDLKQLFSLAEKFG 223
             +E   +L+  G+ ED +  +  + S  + + ++  I G   E ++ +  +  L   +G
Sbjct: 197 SREEFVDQLRSAGIGEDGVSMIADLCSGTRGIDEMARITGKSSEEIARMAAVEDLLASYG 256

Query: 224 YSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGF 283
             + +++D SIVRGL+YYTGIVFE +DR G+ RAI GGGRYD L S   G+ +PA GFG 
Sbjct: 257 VKN-VRYDFSIVRGLSYYTGIVFEAYDRSGQFRAILGGGRYDNLASLMSGESVPAVGFGM 316

Query: 284 GDAVIVELLKEKKL-LPELNVEVENIVCALDPDLQGAASRVATILREKGQTVDLVLENKP 343
           GDAVI  LLK + + +P     V   +C +        +  +  LRE+G  V + +  + 
Sbjct: 317 GDAVISLLLKRENVQIPREKKSV--YICRVGKINSSIMNEYSRKLRERGMNVTVEIMERG 376

Query: 344 LKWVFKRAARINAQRLILVGSSEWQRGMVGVKDLSSGEQREVKLEEL 389
           L    K A+ I A   ++ G  + +RG+V ++++ +G Q  V L+ +
Sbjct: 377 LSAQLKYASAIGADFAVIFGERDLERGVVTIRNMYTGSQENVGLDSV 417

BLAST of Carg10436 vs. Swiss-Prot
Match: sp|Q9UY31|SYH_PYRAB (Histidine--tRNA ligase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=hisS PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.9e-32
Identity = 97/295 (32.88%), Postives = 154/295 (52.20%), Query Frame = 0

Query: 104 AEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKI 163
           A+AE+++  V  +   GL   D    +  R +L E  K  G+ +++   +  IIDK +K+
Sbjct: 134 ADAEVIALFVDSYLSTGL--KDFTVNIGDRVLLDEFAKMLGVKDDI--GLMRIIDKKDKL 193

Query: 164 PLDEMKKELKVVGVSEDAIEEMLQVLSVK----SLTKLEEILGGGGEAMSDLKQLFSLAE 223
             +E  K L   G+ E+ IE++L ++ +K     +  L E L     A  ++ +L++L +
Sbjct: 194 SQEEFLKALGEFGLDENGIEKVLNLIEIKGKPDDVLPLAEELFTSERAKEEISRLYNLVD 253

Query: 224 KFGY---SDWIQFDASIVRGLAYYTGIVFEGF---DRGGKLRAICGGGRYDRLLSTFGGD 283
              +    +WIQ D  I RG  YYT IVFE     D G  + +I GGGRYD L+  FGG 
Sbjct: 254 ILSWYEVDEWIQIDLGIARGFDYYTSIVFEAIVPNDLG--IGSIGGGGRYDNLIEVFGGK 313

Query: 284 DLPACGFGFGDAVIVELLKEKKLLPELNVEVENIVCALDPDLQGAASRVATILREKGQTV 343
             PA GF  G   ++ +L+ K LLPEL    +  V  +  D +  A+ + T LR+ G   
Sbjct: 314 PTPATGFAIGIERLIPILEWKGLLPELKAGPDVFVIPVG-DSRDVATAIVTRLRKAGIRS 373

Query: 344 DLVLENKPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKDLSSGEQREVKLEEL 389
           D+ L  + L+     A RI  +  I+VG  + +RG+V ++DL SG Q EV ++ +
Sbjct: 374 DIELSGRKLRKALDYANRIGVRLSIIVGKRDLERGVVTIRDLESGNQVEVPVDNV 421

BLAST of Carg10436 vs. TrEMBL
Match: tr|A0A1S3CHK2|A0A1S3CHK2_CUCME (histidine--tRNA ligase, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=LOC103500830 PE=3 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 1.9e-166
Identity = 336/507 (66.27%), Postives = 356/507 (70.22%), Query Frame = 0

Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVXXX 60
           MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILR PFPK SFF NR F+SSL+AV   
Sbjct: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFVNRHFYSSLTAVSSA 60

Query: 61  XXXXXXXXXXXXXXALSPTLVTEELQKID------------------------------- 120
                         ALSP   TEELQ+ID                               
Sbjct: 61  QSISDGGTTRGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRL 120

Query: 121 -----------------------------------GHKR--------------------- 180
                                              G++R                     
Sbjct: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 -----------------SVTHVR--------------PDSGAEAELLSSIVAFFKRIGLT 240
                             +T  R              P+  AEAELLSSIV FFKR+G+T
Sbjct: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPEVTAEAELLSSIVTFFKRVGIT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           A+DVGFKVSSRKVLQ+VLKCYGIP+NMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EE+LQVLSVKSLTKLEE+LGGGGEA+SDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 390
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDD+PACGFGFGDAVIVELLKEKKLLPELN 
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420

BLAST of Carg10436 vs. TrEMBL
Match: tr|A0A0A0KER6|A0A0A0KER6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G446550 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 9.5e-166
Identity = 334/507 (65.88%), Postives = 355/507 (70.02%), Query Frame = 0

Query: 1   MAAKPSSLLIFQPRLNLRLKPFIFSSSSLAFSIILRIPFPKASFFGNRKFHSSLSAVXXX 60
           MAAKPSSLLIFQP LNLRLKPFIFSSSSLAFSIILR PFPK SFF NR FHSSL+AV   
Sbjct: 1   MAAKPSSLLIFQPSLNLRLKPFIFSSSSLAFSIILRFPFPKPSFFLNRNFHSSLTAVSSA 60

Query: 61  XXXXXXXXXXXXXXALSPTLVTEELQKID------------------------------- 120
                         ALSP   TEELQ+ID                               
Sbjct: 61  QSVSDGGTTGGRSGALSPAPTTEELQRIDVNPPKGTRDFPPEDMRLRNWLFNNFREVSRV 120

Query: 121 -----------------------------------GHKR--------------------- 180
                                              G++R                     
Sbjct: 121 YGFEEVDYPVLESEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKS 180

Query: 181 -----------------SVTHVR--------------PDSGAEAELLSSIVAFFKRIGLT 240
                             +T  R              P   AEAELLSSIV FFK++G+T
Sbjct: 181 LSLPIKWFAIGQCWRYERMTRGRRREHYQWNMDILGVPGVTAEAELLSSIVTFFKKVGIT 240

Query: 241 AADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300
           A+DVGFKVSSRKVLQ+VLKCYGIP+NMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI
Sbjct: 241 ASDVGFKVSSRKVLQDVLKCYGIPQNMFGKVCIIIDKIEKIPLDEMKKELKVVGVSEDAI 300

Query: 301 EEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360
           EE+LQVLSVKSLTKLEE+LGGGGEA+SDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG
Sbjct: 301 EELLQVLSVKSLTKLEEVLGGGGEALSDLKQLFSLAEKFGYSDWIQFDASIVRGLAYYTG 360

Query: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACGFGFGDAVIVELLKEKKLLPELNV 390
           IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDD+PACGFGFGDAVIVELLKEKKLLPELN 
Sbjct: 361 IVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDIPACGFGFGDAVIVELLKEKKLLPELNA 420

BLAST of Carg10436 vs. TrEMBL
Match: tr|A0A067JZ42|A0A067JZ42_JATCU (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_16551 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 9.9e-139
Identity = 252/289 (87.20%), Postives = 275/289 (95.16%), Query Frame = 0

Query: 100 PDSGAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDK 159
           P+  AEAEL+SSIV FFKRIG+TA+DVGFKVSSRKVLQEVLKCY I EN+FGKVCIIIDK
Sbjct: 221 PEVTAEAELISSIVTFFKRIGITASDVGFKVSSRKVLQEVLKCYSISENLFGKVCIIIDK 280

Query: 160 IEKIPLDEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAE 219
           IEKIP+DE+KKELK VG+SE+A+EE+LQVLS+KSLTKLEEILGG GEA++DLKQLFSLAE
Sbjct: 281 IEKIPIDEIKKELKSVGMSEEAVEELLQVLSIKSLTKLEEILGGAGEAVADLKQLFSLAE 340

Query: 220 KFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACG 279
            FGYS+WIQFDAS+VRGLAYYTGIVFEGFDR GKLRAICGGGRYDRLLSTFGGDDLPACG
Sbjct: 341 NFGYSEWIQFDASVVRGLAYYTGIVFEGFDREGKLRAICGGGRYDRLLSTFGGDDLPACG 400

Query: 280 FGFGDAVIVELLKEKKLLPELNVEVENIVCALDPDLQGAASRVATILREKGQTVDLVLEN 339
           FGFGDAVIVELLKEK LLPEL+++VENIVCALDPDLQGAAS VATILREKGQTVDLVLEN
Sbjct: 401 FGFGDAVIVELLKEKGLLPELSLQVENIVCALDPDLQGAASTVATILREKGQTVDLVLEN 460

Query: 340 KPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKDLSSGEQREVKLEEL 389
           KPLKWVFKRA RINAQRLILVG++EWQ+GMVGVK LSSGEQ EVKL+EL
Sbjct: 461 KPLKWVFKRAGRINAQRLILVGNTEWQKGMVGVKILSSGEQYEVKLDEL 509

BLAST of Carg10436 vs. TrEMBL
Match: tr|A0A2C9VDN9|A0A2C9VDN9_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_08G010700 PE=3 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 1.6e-136
Identity = 244/289 (84.43%), Postives = 275/289 (95.16%), Query Frame = 0

Query: 100 PDSGAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDK 159
           P+  AEAEL+SSIV FFKRIG+TA+DVGFKVSSRKVLQEVLKCY I EN+FGKVCIIIDK
Sbjct: 207 PEVTAEAELISSIVTFFKRIGITASDVGFKVSSRKVLQEVLKCYSISENLFGKVCIIIDK 266

Query: 160 IEKIPLDEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAE 219
           I+KIP+DE+KK+LK VG+SEDA+EE+LQVLS+KSLTKLEEILGG GEA+SDLKQLF LAE
Sbjct: 267 IDKIPIDEIKKDLKSVGMSEDAVEELLQVLSIKSLTKLEEILGGAGEALSDLKQLFFLAE 326

Query: 220 KFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACG 279
           KFGYS+WIQFDAS+VRGLAYYTG+VFEGFDR GKLRAICGGGRYDRLLSTFGGDD+PACG
Sbjct: 327 KFGYSEWIQFDASVVRGLAYYTGVVFEGFDREGKLRAICGGGRYDRLLSTFGGDDIPACG 386

Query: 280 FGFGDAVIVELLKEKKLLPELNVEVENIVCALDPDLQGAASRVATILREKGQTVDLVLEN 339
           FGFGDAVIVELLKEK +LPE++++VENIVCALDPDLQG A+ VAT+LR+KGQ+VDLVLEN
Sbjct: 387 FGFGDAVIVELLKEKGILPEVSLQVENIVCALDPDLQGVAATVATLLRDKGQSVDLVLEN 446

Query: 340 KPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKDLSSGEQREVKLEEL 389
           KPLKWVFKRAARINAQRLILVG++EWQ+GMVGVK LSSGEQ EVKL+EL
Sbjct: 447 KPLKWVFKRAARINAQRLILVGNTEWQKGMVGVKILSSGEQYEVKLDEL 495

BLAST of Carg10436 vs. TrEMBL
Match: tr|B9S0H3|B9S0H3_RICCO (Histidyl-tRNA synthetase, putative OS=Ricinus communis OX=3988 GN=RCOM_1354890 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 8.6e-135
Identity = 245/289 (84.78%), Postives = 271/289 (93.77%), Query Frame = 0

Query: 100 PDSGAEAELLSSIVAFFKRIGLTAADVGFKVSSRKVLQEVLKCYGIPENMFGKVCIIIDK 159
           P   AEAEL+SSIV FFKRIG+TA+DVGFKVSSRKVLQEVL+CY I +N+FGKVCIIIDK
Sbjct: 214 PGVTAEAELISSIVTFFKRIGITASDVGFKVSSRKVLQEVLRCYSISDNLFGKVCIIIDK 273

Query: 160 IEKIPLDEMKKELKVVGVSEDAIEEMLQVLSVKSLTKLEEILGGGGEAMSDLKQLFSLAE 219
           IEKIP+ E+KKELK VG+SE+A+EE+LQVLS+KSLTKLEEILGG GEA+ DLKQLFSLAE
Sbjct: 274 IEKIPVAEIKKELKSVGMSEEAVEELLQVLSIKSLTKLEEILGGAGEAIGDLKQLFSLAE 333

Query: 220 KFGYSDWIQFDASIVRGLAYYTGIVFEGFDRGGKLRAICGGGRYDRLLSTFGGDDLPACG 279
           KFGYS+WIQFDAS+VRGLAYYTGIVFEGFDR GKLRAICGGGRYDRLLSTFGGDDLPACG
Sbjct: 334 KFGYSEWIQFDASVVRGLAYYTGIVFEGFDREGKLRAICGGGRYDRLLSTFGGDDLPACG 393

Query: 280 FGFGDAVIVELLKEKKLLPELNVEVENIVCALDPDLQGAASRVATILREKGQTVDLVLEN 339
           FGFGDAVIVELLKEK LLPEL ++VENIVCALD DLQGAA+RVATILREKGQ VDLVLE+
Sbjct: 394 FGFGDAVIVELLKEKGLLPELGLQVENIVCALDSDLQGAAARVATILREKGQNVDLVLED 453

Query: 340 KPLKWVFKRAARINAQRLILVGSSEWQRGMVGVKDLSSGEQREVKLEEL 389
           KPLKWVFKRAARINAQ+L+LVG +EWQ+GMVGVK LS+GEQ EVKL+EL
Sbjct: 454 KPLKWVFKRAARINAQKLVLVGKTEWQKGMVGVKILSTGEQYEVKLDEL 502

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023544233.13.6e-17774.16histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita pepo s... [more]
XP_022925718.16.2e-17774.36histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita moscha... [more]
XP_022978407.13.1e-17670.61histidine--tRNA ligase, chloroplastic/mitochondrial isoform X2 [Cucurbita maxima... [more]
XP_023544232.12.0e-17573.58histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita pepo s... [more]
XP_022925717.13.4e-17573.78histidine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita moscha... [more]
Match NameE-valueIdentityDescription
AT3G46100.11.1e-12476.82Histidyl-tRNA synthetase 1[more]
AT3G02760.11.6e-2230.58Class II aaRS and biotin synthetases superfamily protein[more]
AT5G03406.12.0e-2067.16Class II aaRS and biotin synthetases superfamily protein[more]
Match NameE-valueIdentityDescription
sp|O82413|SYHM_ARATH2.1e-12376.82Histidine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3... [more]
sp|B1ZUW6|SYH_OPITP1.7e-3735.33Histidine--tRNA ligase OS=Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1... [more]
sp|Q8U431|SYH_PYRFU1.5e-3632.54Histidine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JC... [more]
sp|Q9HLX5|SYH_THEAC4.7e-3532.06Histidine--tRNA ligase OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728... [more]
sp|Q9UY31|SYH_PYRAB2.9e-3232.88Histidine--tRNA ligase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=hi... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3CHK2|A0A1S3CHK2_CUCME1.9e-16666.27histidine--tRNA ligase, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=L... [more]
tr|A0A0A0KER6|A0A0A0KER6_CUCSA9.5e-16665.88Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G446550 PE=3 SV=1[more]
tr|A0A067JZ42|A0A067JZ42_JATCU9.9e-13987.20Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_16551 PE=3 SV=1[more]
tr|A0A2C9VDN9|A0A2C9VDN9_MANES1.6e-13684.43Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_08G010700 PE=3 SV=... [more]
tr|B9S0H3|B9S0H3_RICCO8.6e-13584.78Histidyl-tRNA synthetase, putative OS=Ricinus communis OX=3988 GN=RCOM_1354890 P... [more]
The following terms have been associated with this gene:
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: INTERPRO
TermDefinition
IPR004154Anticodon-bd
IPR004516HisRS/HisZ
IPR036621Anticodon-bd_dom_sf
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10436-RACarg10436-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036621Anticodon-binding domain superfamilyGENE3DG3DSA:3.40.50.800coord: 298..389
e-value: 2.5E-18
score: 67.6
NoneNo IPR availablePFAMPF13393tRNA-synt_Hiscoord: 104..281
e-value: 3.1E-20
score: 72.6
NoneNo IPR availableGENE3DG3DSA:3.30.930.10coord: 99..297
e-value: 1.0E-57
score: 197.2
NoneNo IPR availablePANTHERPTHR43707:SF1HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATEDcoord: 101..388
NoneNo IPR availableSUPERFAMILYSSF55681Class II aaRS and biotin synthetasescoord: 100..293
NoneNo IPR availableSUPERFAMILYSSF52954Class II aaRS ABD-relatedcoord: 304..388
IPR004516Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunitPIRSFPIRSF001549His-tRNA_synthcoord: 99..389
e-value: 7.4E-82
score: 273.3
IPR004516Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunitPANTHERPTHR43707FAMILY NOT NAMEDcoord: 101..388
IPR004154Anticodon-bindingPFAMPF03129HGTP_anticodoncoord: 310..388
e-value: 7.4E-9
score: 35.6

The following gene(s) are paralogous to this gene:

None