Carg09270 (gene) Silver-seed gourd

NameCarg09270
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionAlpha/beta hydrolase, putative
LocationCucurbita_argyrosperma_scaffold_018 : 309331 .. 313125 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAAAAAAAAAAATTACCTCAACCAATCCATGAATTTAGGATTTAGTAGAGTTTGTAATTTAACCGGGAAATAAGACAGCCCATCGTTTGGCTGTGGATAGAAAAGCAGGAGAAGAGAGGCCAACGGGCGCCAATTTGCCCGCGCTTAACAGAGATGCTCTCTCTAGCAGTGGCTGCGAGTTGGTGCTCCGCTCCACCGCTCATGCAATGGAAGAAACCGAAGCGTCTGGGGGTCATCCTTAACAAATTCCCTTCATTTCTTCCCAAAGAAGTCGACAATATCAAAGACCAATTTGCTCGGGAGCTCGCTACAAGGATCGAGAGGCTACCAGTAAGTTCACTGCTTCTACCTCTCTTGAAAATTCGTACTATTTGAGAATTCTAGTTGTTTGAAAGTCATCTGATGATTATTTCACATGGACTGGAGAAATTTATAATAGAAACTCACGATTCGAGGAAGTTAAAGCCATGTTTCTTGCGGAATTCTGTTTGTCAATGTGATTAATCGAAGGGTTTTAATTTCTACTAAACGTTATGAGATTTGGCAGTGTTCTTGATCTGATTTATATGTTTGTTCTTGCTCTGCATGCAGGTCAGCTTCTCCGATTCTTGTACTATGAGTAGTTGCGTGAAGCCGTCGATACAGAGCAAAGAATGTCCAGTGGTTCTTCTCCATGGATTTGATAGGTTTCTTTCACTAATTAGAAACAATATTCTTTCAAAATACAAGGCTGTTTGTTGTTTCTGTGATATTTGTTATTATTATATCTTTTCCTCGTTCATCACATCTTATTATTCAGCTCCTGTTTAGAATGGAGATACACATATCCATTGCTTGAGGAAGCTGGTTTTGAAACCTGGGCTGTTGATATTCTTGGCTGGGGCTTCTCTGATTTAGGTCTGTTTAATTGCTATATTCCTTTTTTAATTGATCGTTAAACCATTATTTTTATCTCTAATTTTCATAGTTGCATGTGATAGAAAGACTCCCTCCATGTGACGTGACTTCTAAACGTGTTCATTTGTATCAGGTAATGAAAACGTTCTGTTTAGATCGTTTGCCTATTACGAATTTGGGGAAAATTTCTCTTCAATCCCAATTCTGCTACCTTTCAATATGATCATAATATGAACTCTCCAAGCACTTATCTTGTTTGTGCTCTTTTAATTACTATGTTAATACTTGCATCATTGTTATAAGCTTGTTTTATTTTGTCATGATTGCTATGAACTTTTCATTAATATCATTGTCAATCACACTCATCTTGTCTTGATATGTTGAAACTAATTTAGTTTTGCTTCGTCCTGATAATAGCTTTGGAAATCATATATTAAAAGGCCAATGGTATTGGTTGGACCAAGCCTTGGTGCTGCTGTTGCTATTGATTTTGCTGTCAATTATCCAGAAGCTGTACGTATCTCTTAGCTGTAACTTATGACTACAAATTTGAAGCTATATTATCACATGAAGTTATTGAGAGTTTTCCGTCGAATGTAACGAGATTACTTCAGGTCGATAGGCTTGTGTTGATTGATGCAAGTGTATATGCAGAAGGTACAGGAAACTTAGCTACCCTACCAAGATTGATTGCCTATGCTGGGGTGAGTCTTTGCTCACTTCTGTAAACATTCCATCAAATATCTTAATGCTAAGCATTTTTACCGAGTGTTCGATTTTCTGCAATTACAAGACAACCATAACTTTTCCTTCATCTGTACATCCCTTTTCCATCGAAGGAAGGTATCATTATGCCACATGTAACAGCCCAAACCCGCTAGCAGATATTGTCCTCTTTGGGCTTTTCCTCTAGGTTTTAAAACGGGTTTGCTGGGGGAGAGATTTCCACACTTTTATAAAAAATGCTTCATTCCCCTCTCCAACCGACGTGGGATCTCACGCCACCACTACTAGCTTCCATAATAATTCTTCTGTTAAAATATTCTCGAAGAGAATCATTCCTCTAATTTTCGATTTACAAATTTGATCTTTTGTAACGCAATTTGCAGTTCCATGGAGGAAAGATTTTTACCCACTTTTCTTCTTTTCTAAAATATCTTCTTGGAGAGAATTGATTGCGTTTTTTGGTTTGTAATGTATAGTTGTAATTGTTTAACTTATTTTGATCAGGTATTTCTATTGAAGAGCATTCCCCTGCGCTTATATGTGAACGTTTTGGCCTTCACTGGCATATCACTTAGTACTAGCCTAGATTGGGTCAATGTAAGTTTTCCCCTACATCAAGGAGGATATATATCATATATTTGTTGACTTGCCTGAGTACTTTGTGAAATTGGAAGGCTTAATTGACATAAAGCTAAAATGCTCTGTGTCCATGGCCAATTGACTCACCCCCTGCTCCCACTGTTCTCCTTCTATCCCTCCTTATTAGATTGGCCGCCTGCACTGTTTATTACCTTGGTGGGAGGATGCGACTGTCAGTTTTATGTTAAGTGGAGGATATAATGTCAGCAGCCAGATTGAAAAGGTTAGTACAATTCTTTATGCTATTATATGGTTGATGCAAAATGCAATGTTCTAAGTCACGACGTATCGAGTTCTTAGTATTTTAAATTGCAAGAATGTTAGTGAGGGACATAAGGAAAAGGTACGGTTCCTCATCAAGGTTCGTCGAATTGAATTTTCCCTAAGGTCTTGAATGAATAACTACTAGTGTCTTTTCATATGCTTCATTTTTCAATTCTCATATCCACATTTGAGGATCTTTGGGTACTGATTATTACTTGCATTTTTGTTTTATAGGTGAAGCAGAAAACGCTTATTATATGGGGTGAGGATGATCAGATCATCAGCAACAAGCTTGCAGTGGTTAGTGTTTACTGTTTATTGCAATTCTGAAAAATGAGATTGAGATAAAAAATTGAATTGGGTTGACAGAAAAGACTGATAAACAGAGAAAGATCTAGAACTATAGATTGTTTTTTTTTTCCTTTCATAAGAACTGTAGATTATTTTTATGCGATCTTTTAAACAAAACTTAAAGATAAAACTAATACAAAAGTTAAAACATCATATATTTATAATTACTATCGTATGTAGATCATAAATAAGTATTGTTAGAAATGGAATAAGCTCACATTTTTGGAGAATGCAGAGGCTGCACTGTGAACTGCCAAATGCATTCATTCGTCAAATACCTGAGTGCGGCCACCTTCCTCATGTGGAAAAGCCAAATTTTGTTGCCAAATTGATTACTCAATTTGTTCATGAAGATTCCAGAAAAGAGGTTCCGTATGTTAATAATGCATAATTTTTTTTTATTTTTTATTTTTGTTGGATTTGTTTTATAATATGATGGTGTGTTATGTGTATATTTATTTTTTGTATTTATAATATTTCTTCTGTTATTGGCTTATGTTCTTTGATCCATTCGGTAGCGCAAAACCCTCATTGGCTAAACAATTTTCGGACCATCAAACTTGTAAGCTTAGTCTTCACATACTTCCATTTCTAAATCTAACTCGAGCGCAATTTTCTGTAAGGTGGTAGGTCGATTTGAACGCTTTAACTTGGCCACTGAAGTAGGCAAAGACTTGACAAAGGTTAGGTTCAATTATTGCCTTTTCAAATCATGAAGTACTACTCTTAGTATGCCTACACGTTTTAAAACTCTAGCAGCGCTGGGCTGCGAGACTCATTGTTGAATCCTATTTGAAATAGATACTCCCTTTCCACAAATCTCCTCAGTAGCAAGCACATAGGACTAACTCTTTTAGGGCATCGTAAGCGTGGCCACAACAGTTCGTATTGATCAGTGCAAGTATGTGCAGTCA

mRNA sequence

ATGCCCATCGTTTGGCTGTGGATAGAAAAGCAGGAGAAGAGAGGCCAACGGGCGCCAATTTGCCCGCGCTTAACAGAGATGCTCTCTCTAGCAGTGGCTGCGAGTTGGTGCTCCGCTCCACCGCTCATGCAATGGAAGAAACCGAAGCGTCTGGGGGTCATCCTTAACAAATTCCCTTCATTTCTTCCCAAAGAAGTCGACAATATCAAAGACCAATTTGCTCGGGAGCTCGCTACAAGGATCGAGAGGCTACCAGTCAGCTTCTCCGATTCTTGTACTATGAGTAGTTGCGTGAAGCCGTCGATACAGAGCAAAGAATGTCCAGTGGTTCTTCTCCATGGATTTGATAGCTCCTGTTTAGAATGGAGATACACATATCCATTGCTTGAGGAAGCTGGTTTTGAAACCTGGGCTGTTGATATTCTTGGCTGGGGCTTCTCTGATTTAGAAAGACTCCCTCCATGTGACGTGACTTCTAAACGTGTTCATTTGTATCAGCTTTGGAAATCATATATTAAAAGGCCAATGGTATTGGTTGGACCAAGCCTTGGTGCTGCTGTTGCTATTGATTTTGCTGTCAATTATCCAGAAGCTGTCGATAGGCTTGTGTTGATTGATGCAAGTGTATATGCAGAAGGTACAGGAAACTTAGCTACCCTACCAAGATTGATTGCCTATGCTGGGGTATTTCTATTGAAGAGCATTCCCCTGCGCTTATATGTGAACGTTTTGGCCTTCACTGGCATATCACTTAGTACTAGCCTAGATTGGGTCAATATTGGCCGCCTGCACTGTTTATTACCTTGGTGGGAGGATGCGACTGTCAGTTTTATGTTAAGTGGAGGATATAATGTCAGCAGCCAGATTGAAAAGGTGAAGCAGAAAACGCTTATTATATGGGGTGAGGATGATCAGATCATCAGCAACAAGCTTGCAGTGAGGCTGCACTGTGAACTGCCAAATGCATTCATTCGTCAAATACCTGAGTGCGGCCACCTTCCTCATGTGGAAAAGCCAAATTTTGTTGCCAAATTGATTACTCAATTTGTTCATGAAGATTCCAGAAAAGAGGTTCCGTATGTTAATAATGCATAATTTTTTTTTATTTTTTATTTTTGTTGGATTTGTTTTATAATATGATGGTGTGTTATGTGTATATTTATTTTTTGTATTTATAATATTTCTTCTGTTATTGGCTTATGTTCTTTGATCCATTCGGTAGCGCAAAACCCTCATTGGCTAAACAATTTTCGGACCATCAAACTTGTAAGCTTAGTCTTCACATACTTCCATTTCTAAATCTAACTCGAGCGCAATTTTCTGTAAGGTGGTAGGTCGATTTGAACGCTTTAACTTGGCCACTGAAGTAGGCAAAGACTTGACAAAGGTTAGGTTCAATTATTGCCTTTTCAAATCATGAAGTACTACTCTTAGTATGCCTACACGTTTTAAAACTCTAGCAGCGCTGGGCTGCGAGACTCATTGTTGAATCCTATTTGAAATAGATACTCCCTTTCCACAAATCTCCTCAGTAGCAAGCACATAGGACTAACTCTTTTAGGGCATCGTAAGCGTGGCCACAACAGTTCGTATTGATCAGTGCAAGTATGTGCAGTCA

Coding sequence (CDS)

ATGCCCATCGTTTGGCTGTGGATAGAAAAGCAGGAGAAGAGAGGCCAACGGGCGCCAATTTGCCCGCGCTTAACAGAGATGCTCTCTCTAGCAGTGGCTGCGAGTTGGTGCTCCGCTCCACCGCTCATGCAATGGAAGAAACCGAAGCGTCTGGGGGTCATCCTTAACAAATTCCCTTCATTTCTTCCCAAAGAAGTCGACAATATCAAAGACCAATTTGCTCGGGAGCTCGCTACAAGGATCGAGAGGCTACCAGTCAGCTTCTCCGATTCTTGTACTATGAGTAGTTGCGTGAAGCCGTCGATACAGAGCAAAGAATGTCCAGTGGTTCTTCTCCATGGATTTGATAGCTCCTGTTTAGAATGGAGATACACATATCCATTGCTTGAGGAAGCTGGTTTTGAAACCTGGGCTGTTGATATTCTTGGCTGGGGCTTCTCTGATTTAGAAAGACTCCCTCCATGTGACGTGACTTCTAAACGTGTTCATTTGTATCAGCTTTGGAAATCATATATTAAAAGGCCAATGGTATTGGTTGGACCAAGCCTTGGTGCTGCTGTTGCTATTGATTTTGCTGTCAATTATCCAGAAGCTGTCGATAGGCTTGTGTTGATTGATGCAAGTGTATATGCAGAAGGTACAGGAAACTTAGCTACCCTACCAAGATTGATTGCCTATGCTGGGGTATTTCTATTGAAGAGCATTCCCCTGCGCTTATATGTGAACGTTTTGGCCTTCACTGGCATATCACTTAGTACTAGCCTAGATTGGGTCAATATTGGCCGCCTGCACTGTTTATTACCTTGGTGGGAGGATGCGACTGTCAGTTTTATGTTAAGTGGAGGATATAATGTCAGCAGCCAGATTGAAAAGGTGAAGCAGAAAACGCTTATTATATGGGGTGAGGATGATCAGATCATCAGCAACAAGCTTGCAGTGAGGCTGCACTGTGAACTGCCAAATGCATTCATTCGTCAAATACCTGAGTGCGGCCACCTTCCTCATGTGGAAAAGCCAAATTTTGTTGCCAAATTGATTACTCAATTTGTTCATGAAGATTCCAGAAAAGAGGTTCCGTATGTTAATAATGCATAA

Protein sequence

MPIVWLWIEKQEKRGQRAPICPRLTEMLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPVSFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNNA
BLAST of Carg09270 vs. NCBI nr
Match: XP_022962117.1 (uncharacterized protein LOC111462669 isoform X1 [Cucurbita moschata])

HSP 1 Score: 698.4 bits (1801), Expect = 1.3e-197
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF
Sbjct: 61  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNNA 365
           IPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNNA
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNNA 338

BLAST of Carg09270 vs. NCBI nr
Match: XP_022962121.1 (uncharacterized protein LOC111462669 isoform X3 [Cucurbita moschata])

HSP 1 Score: 684.9 bits (1766), Expect = 1.5e-193
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF
Sbjct: 61  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 359
           IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 332

BLAST of Carg09270 vs. NCBI nr
Match: XP_022962120.1 (uncharacterized protein LOC111462669 isoform X2 [Cucurbita moschata])

HSP 1 Score: 683.7 bits (1763), Expect = 3.4e-193
Identity = 331/332 (99.70%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF
Sbjct: 61  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 359
           IPECGHLPHVEKPNFVAKLITQFVHEDSRKE+
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEL 332

BLAST of Carg09270 vs. NCBI nr
Match: XP_022997199.1 (uncharacterized protein LOC111492171 isoform X2 [Cucurbita maxima])

HSP 1 Score: 681.0 bits (1756), Expect = 2.2e-192
Identity = 329/332 (99.10%), Postives = 331/332 (99.70%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLSLAVAASWCSAPPLMQWKKPKRLGVI+NKFPSFLPKEVDNIKDQFARELATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVIINKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSC MSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF
Sbjct: 61  SFSDSCIMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMV+VGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVMVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 359
           IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 332

BLAST of Carg09270 vs. NCBI nr
Match: XP_022997198.1 (uncharacterized protein LOC111492171 isoform X1 [Cucurbita maxima])

HSP 1 Score: 679.9 bits (1753), Expect = 4.9e-192
Identity = 328/332 (98.80%), Postives = 331/332 (99.70%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLSLAVAASWCSAPPLMQWKKPKRLGVI+NKFPSFLPKEVDNIKDQFARELATRIERLPV
Sbjct: 1   MLSLAVAASWCSAPPLMQWKKPKRLGVIINKFPSFLPKEVDNIKDQFARELATRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSC MSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF
Sbjct: 61  SFSDSCIMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMV+VGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVMVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ
Sbjct: 241 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEV 359
           IPECGHLPHVEKPNFVAKLITQFVHEDSRKE+
Sbjct: 301 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEL 332

BLAST of Carg09270 vs. TAIR10
Match: AT1G13820.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 471.9 bits (1213), Expect = 3.7e-133
Identity = 230/313 (73.48%), Postives = 258/313 (82.43%), Query Frame = 0

Query: 53  VILNKFPSFLPKEVDNIKDQFARELATRIERLP--VSFSDSCTMSSCVKPSIQSKECPVV 112
           V   KFP+FLP +V+ IKD FA +LA RIERLP  VSF +   MSSCV P ++++  PVV
Sbjct: 27  VTAEKFPTFLPTDVEKIKDTFALKLAARIERLPVSVSFREDSIMSSCVTPLMRNETTPVV 86

Query: 113 LLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDVTSKRVHLYQLWKS 172
           LLHGFDSSCLEWRYTYPLLEEAG ETWA DILGWGFSDL++LPPCDV SKR H Y+ WKS
Sbjct: 87  LLHGFDSSCLEWRYTYPLLEEAGLETWAFDILGWGFSDLDKLPPCDVASKREHFYKFWKS 146

Query: 173 YIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLATLPRLIAYAGVF 232
           +IKRP+VLVGPSLGAAVAID AVN+PEAV+ LVL+DASVYAEGTGNLATLP+  AYAGV+
Sbjct: 147 HIKRPVVLVGPSLGAAVAIDIAVNHPEAVESLVLMDASVYAEGTGNLATLPKAAAYAGVY 206

Query: 233 LLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWWEDATVSFMLSGGYNVSSQIE 292
           LLKSIPLRLYVN + F GISL TS DW  IGRLHCL PWWEDATVSFM SGGYNV+S I+
Sbjct: 207 LLKSIPLRLYVNFICFNGISLETSWDWTKIGRLHCLYPWWEDATVSFMTSGGYNVTSLIK 266

Query: 293 KVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPECGHLPHVEKPNFVAKLITQFV 352
           KV QKTLI+WGEDDQIISNKLA RLH EL NA ++QI  CGHLPHVEKP  V KLI +FV
Sbjct: 267 KVSQKTLILWGEDDQIISNKLAWRLHGELSNARVKQISNCGHLPHVEKPAAVTKLIAEFV 326

Query: 353 HEDSR-KEVPYVN 363
            E  R KEV  ++
Sbjct: 327 RETCRCKEVESIS 339

BLAST of Carg09270 vs. TAIR10
Match: AT5G39220.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 412.9 bits (1060), Expect = 2.0e-115
Identity = 195/316 (61.71%), Postives = 240/316 (75.95%), Query Frame = 0

Query: 39  APPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPVSFS----DSCTM 98
           +PP    +  +R+    + FP+FLPKE+ NIKD FAR LA RI R+PV           M
Sbjct: 9   SPPPPATRFRQRVTANGDGFPTFLPKEIQNIKDPFARALAQRIVRIPVPLQMGNFRGSVM 68

Query: 99  SSCVKPSIQ-SKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLERLP 158
           SSC+KP +Q   + PVVLLH FDSSCLEWR TYPLLE+A  ETWA+D+LGWGFSDLE+LP
Sbjct: 69  SSCIKPLVQLHDKSPVVLLHCFDSSCLEWRRTYPLLEQACLETWAIDVLGWGFSDLEKLP 128

Query: 159 PCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEG 218
           PCD  SKR HL++LWK+YIKRPM+LVGPSLGA VA+DF   YPEAVD+LVLI+A+ Y+EG
Sbjct: 129 PCDAASKRHHLFELWKTYIKRPMILVGPSLGATVAVDFTATYPEAVDKLVLINANAYSEG 188

Query: 219 TGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWWEDA 278
           TG L  LP+ IAYAGV LLKS PLRL  NVLAF    LS ++DW NIGRLHC +PWWEDA
Sbjct: 189 TGRLKELPKSIAYAGVKLLKSFPLRLLANVLAFCSSPLSENIDWTNIGRLHCQMPWWEDA 248

Query: 279 TVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPECGHL 338
            V FM+SGGYNV+S I+ +  KTL++  E+DQI+SN+L+V+L CEL NA +R++P+ GHL
Sbjct: 249 MVDFMISGGYNVASHIKHIDHKTLVVCSENDQIVSNQLSVKLLCELANAVLREVPDSGHL 308

Query: 339 PHVEKPNFVAKLITQF 350
           PHVE P  + KLI+ F
Sbjct: 309 PHVENPKHIVKLISDF 324

BLAST of Carg09270 vs. TAIR10
Match: AT4G36530.2 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 82.4 bits (202), Expect = 6.3e-16
Identity = 68/271 (25.09%), Postives = 116/271 (42.80%), Query Frame = 0

Query: 102 IQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDVTSKR 161
           +Q +  P+VL+HGF +S   WRY  P L +  ++ +A+D+LG+G+SD + L   D     
Sbjct: 95  VQGEGSPLVLIHGFGASVFHWRYNIPELAKK-YKVYALDLLGFGWSD-KALIEYDAMVWT 154

Query: 162 VHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDAS--VYAEGTGNLAT 221
             +    K  +K P V+VG SLG   A+  AV  PE V  + L++++    AE       
Sbjct: 155 DQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALLNSAGQFAAESRKREEA 214

Query: 222 LPRLIAYAGVFLLKSIPLRLYVNVL--------AFTGISLSTSLDWVNIGRL-------- 281
              +I    V  LK I  R+ +  L            +  S  +D  N+           
Sbjct: 215 DETVITKFIVKPLKEIFQRVVLGFLFWQAKQPSRIESVLKSVYIDSTNVDDYLVESISKP 274

Query: 282 ----HCLLPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCEL 341
               +    ++   T        Y + S + K+    L++WG+ D  +    A ++    
Sbjct: 275 ATDPNAGEVYYRLMTRFLTNQSRYTLDSVLSKMTCPLLLVWGDLDPWVGPAKAEKIKAFY 334

Query: 342 PNAFIRQIPECGHLPHVEKPNFVAKLITQFV 351
            N+ +  + + GH PH E P  V K +  ++
Sbjct: 335 SNSSLVHL-QAGHCPHDEVPEAVNKALLDWL 362

BLAST of Carg09270 vs. TAIR10
Match: AT5G09430.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 73.2 bits (178), Expect = 3.8e-13
Identity = 75/273 (27.47%), Postives = 122/273 (44.69%), Query Frame = 0

Query: 89  SDSCTMSSCVKPSIQSKECP-VVLLHGFDSSCLEWRYTYPLLEEAG-FETWAVDILGWGF 148
           S + T   C  P   ++  P ++LLHGF ++ + W+Y   L    G F  +  D+L +G 
Sbjct: 42  SIASTAMHCWIPKSPNRSKPNLLLLHGFGANAM-WQYGEHLRAFTGRFNVYVPDLLFFGL 101

Query: 149 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 208
           S        +    R  L +L +++  + M +VG S G  V    A  +PE V++LVL  
Sbjct: 102 SSTSEPNRTESFQARC-LMRLMEAHGVQRMNIVGISYGGFVGYSLAAQFPENVEKLVLCC 161

Query: 209 ASVYAEG---TGNLATLPRLIAYAGVFLLKSIPLRL-----YVNVLAFTGISLSTSLDWV 268
           A V  E       L  +P L    G+ L+   P +L     +  V    G+      D++
Sbjct: 162 AGVCLEEKDMEDGLFKVPNLEEATGI-LIPQTPEKLKELIRFSFVKPIKGVPSFFLWDFI 221

Query: 269 NIGRLHCLLPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCE 328
           ++      +    D   S +        S + ++KQK+LIIWGE+DQI   +L  RL   
Sbjct: 222 DVMCTE-FVEEKRDLIKSILKD---RRLSDLPRIKQKSLIIWGEEDQIFPLELGYRLKRH 281

Query: 329 L-PNAFIRQIPECGHLPHVEKPNFVAKLITQFV 351
           +  +A I  I + GH  ++EK     K +  F+
Sbjct: 282 IGESAEIVVIKKAGHAVNLEKSKEFVKHLKSFL 307

BLAST of Carg09270 vs. TAIR10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 69.3 bits (168), Expect = 5.5e-12
Identity = 67/276 (24.28%), Postives = 116/276 (42.03%), Query Frame = 0

Query: 99  KPSIQSKECP-VVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDV 158
           K   +SK  P ++L+HG  ++ +   Y         F  +  D++ +G S   R    D+
Sbjct: 43  KTKPESKPKPNLLLIHGLGATAIWQWYDVARRLSRYFNLYIPDLVFFGGSSTTRPERSDI 102

Query: 159 TSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAE----- 218
              +  +  L    +K+   LVG S G  V    A  Y +AV+++V+  A+V  E     
Sbjct: 103 FQAQTLMRALEAQSVKK-FSLVGLSYGGFVGYRMASMYADAVEKVVICCAAVCVEEKDMK 162

Query: 219 ----GTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCLL- 278
                  +L    +++    V  L+ +   ++        +      D++     H L  
Sbjct: 163 AGVFKVSDLDEASKILVPESVKKLRELMGYIFYKPALARLVPTCLLHDFIE----HALTR 222

Query: 279 PWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCEL-PNAFIRQ 338
              E+            + S+I K+KQ TLIIWGE DQ+   ++  RL   +  N  +  
Sbjct: 223 DNMEEKRELIKAIPKDRIISEIPKLKQPTLIIWGEHDQVFPLEMGKRLEKHVGDNGKLVI 282

Query: 339 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVN 363
           I   GH+ + EKP    KL+  F+ E S+ ++P  N
Sbjct: 283 IKRTGHIFNFEKPKKFIKLLKSFLLETSKPQIPVSN 313

BLAST of Carg09270 vs. Swiss-Prot
Match: sp|O05235|YUGF_BACSU (Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yugF PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.9e-15
Identity = 61/246 (24.80%), Postives = 117/246 (47.56%), Query Frame = 0

Query: 109 VVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDVTSKRVHLYQLW 168
           +V +HGF SS   +R   PLL +  ++  A+D+  +G S+  R       +    +  + 
Sbjct: 30  LVCVHGFLSSAFSFRKVIPLLRDK-YDIIALDLPPFGQSEKSRTFIYTYQNLAKLVIGIL 89

Query: 169 KSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNL----ATLPRLI 228
           +    +  VLVG S+G  +++  A+  PE   ++VL+ +S Y + +         +P   
Sbjct: 90  EHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCSSGYLKRSHPTIIFGTHIPYFH 149

Query: 229 AYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWWEDATVSFMLSGGYN 288
            Y   +L K   ++  +NV+    +     +D    GR       ++  T       G  
Sbjct: 150 LYIKRWLSKEGVMKNLLNVVHDKSLIDEEMID--GYGRPFQDEQIFKAMTRFIRHREGDL 209

Query: 289 VSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPECGHLPHVEKPNFVAK 348
              Q++K+ +  L+IWGE+D+I+  ++  RLH +LPN+ +  + + GHL   E+P  +++
Sbjct: 210 EPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHADLPNSVLYSLGQTGHLVPEERPELISE 269

Query: 349 LITQFV 351
            I  F+
Sbjct: 270 HIADFI 272

BLAST of Carg09270 vs. Swiss-Prot
Match: sp|A4JPX5|MHPC_BURVG (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Burkholderia vietnamiensis (strain G4 / LMG 22486) OX=269482 GN=mhpC PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 8.9e-12
Identity = 66/257 (25.68%), Postives = 110/257 (42.80%), Query Frame = 0

Query: 109 VVLLHGFDSSCLEWRYTYPLLE---EAGFETWAVDILGWGFSD-LERLPPCDVTSKRVHL 168
           +VLLHG       W   +  ++    AG+    VD  GWG SD +         + RV  
Sbjct: 39  LVLLHGSGPGATGWANFHRNVDAFANAGYRVILVDCPGWGKSDSIVCTGSRSDLNARVLA 98

Query: 169 YQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLATLPRLI 228
             L    I R   LVG S+G   A+ FA++YPE V +LVL+       G      +P   
Sbjct: 99  GVLDTLGIGRAH-LVGNSMGGHSAVAFALSYPERVGKLVLMGGG--TGGPSQFVPMPT-- 158

Query: 229 AYAGVFLLKSI-------PLRLYVNVLAFTGISLSTSLDWVN----IGRLHCLLPWWEDA 288
              G+ LL+++        L+  +NV  +   +++  L        +GR   L  + +  
Sbjct: 159 --EGIKLLQALYRDPTLENLKKMLNVFVYDASTMTEELMQTRLENMLGRRDHLENFVKSL 218

Query: 289 TVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPECGHL 348
           T +      Y    ++ ++K   L+IWG DD+ +   + +RL   +PNA +     CGH 
Sbjct: 219 TANPKQFPDY--GHRLSEIKAPALVIWGRDDRFVPMDVGLRLVWNMPNADLHVFGRCGHW 278

Query: 349 PHVEKPNFVAKLITQFV 351
              E      +++ +F+
Sbjct: 279 AQWEHAERFNRMVLEFL 286

BLAST of Carg09270 vs. Swiss-Prot
Match: sp|Q476M7|MHPC_CUPNJ (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Cupriavidus necator (strain JMP 134 / LMG 1197) OX=264198 GN=mhpC PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 8.9e-12
Identity = 70/260 (26.92%), Postives = 112/260 (43.08%), Query Frame = 0

Query: 109 VVLLHGFDSSCLEW----RYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDVTSK---- 168
           VV+LHG       W    R   PL+  AG+    VD  GWG SD    P  +  S+    
Sbjct: 40  VVMLHGSGPGATGWANFNRNVEPLV-AAGYRVLLVDCPGWGKSD----PVVNAGSRSELN 99

Query: 169 -RVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLAT 228
            RV    L +  I+R  +L G S+G   A+ FA+  P+ V +LVL+       GTG   +
Sbjct: 100 GRVLKGVLDELDIERVHIL-GNSMGGHSAVAFALANPQRVGKLVLMGG-----GTGG-PS 159

Query: 229 LPRLIAYAGVFLLKSI-------PLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWWED 288
           L   +   G+ LL  +        L+  +NV  +   SL+  L    +  +       E+
Sbjct: 160 LYAPMPTEGIKLLNGLYREPSIENLKRMMNVFVYDASSLTDDLMQARLDNMLARRDHLEN 219

Query: 289 ATVSFMLSGGY--NVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPEC 348
              S   +     +  S++ ++   TL+IWG DD+ +   + +RL   +PNA +     C
Sbjct: 220 FVKSLAANPKQFTDYGSRLGEITAPTLVIWGRDDRFVPMDVGLRLIAGIPNAQMHIFNRC 279

Query: 349 GHLPHVEKPNFVAKLITQFV 351
           GH    E      +++  F+
Sbjct: 280 GHWAQWEHAKAFNRMVVDFL 287

BLAST of Carg09270 vs. Swiss-Prot
Match: sp|B2JQW2|MHPC_PARP8 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) OX=391038 GN=mhpC PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.2e-11
Identity = 65/255 (25.49%), Postives = 106/255 (41.57%), Query Frame = 0

Query: 109 VVLLHGFDSSCLEWRYTYPLLE---EAGFETWAVDILGWGFSD-LERLPPCDVTSKRVHL 168
           +VLLHG       W   Y  ++    AG+    VD  GWG SD +         + RV  
Sbjct: 39  LVLLHGSGPGASGWANFYRNVDAFANAGYRVILVDCPGWGKSDSIVCTGSRSDLNARVLK 98

Query: 169 YQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLATLPRLI 228
             L    I+R   LVG S+G   A+ FA++YPE V +LVL+       G      +P   
Sbjct: 99  GVLDTLGIERAH-LVGNSMGGHSAVAFALSYPERVGKLVLMGGG--TGGPSQFVPMPT-- 158

Query: 229 AYAGVFLLKSI-------PLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWWEDATVSF 288
              G+ LL+ +        L+  +NV  +   +++  L    +  +       E+   S 
Sbjct: 159 --EGIKLLQGLYRDPTLENLKKMLNVFVYDASTMTEELMQTRLDNMLARRDHLENFVKSL 218

Query: 289 MLSGGY--NVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPECGHLPH 348
             +     +   ++ ++K   L+IWG DD+ +   + +RL   LPNA       CGH   
Sbjct: 219 TANPKQFPDYGHRLNEIKAPALVIWGRDDRFVPLDVGLRLVWGLPNAEFHVFGRCGHWAQ 278

Query: 349 VEKPNFVAKLITQFV 351
            E      +++  F+
Sbjct: 279 WEHAERFNQMLLDFL 286

BLAST of Carg09270 vs. Swiss-Prot
Match: sp|O31581|YFHM_BACSU (AB hydrolase superfamily protein YfhM OS=Bacillus subtilis (strain 168) OX=224308 GN=yfhM PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.6e-11
Identity = 71/265 (26.79%), Postives = 110/265 (41.51%), Query Frame = 0

Query: 109 VVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDL-ERLPPCDVTSKRVHLYQL 168
           +VLLHGF      W+     L +AG+   A D  G+  SD  E +    + + R  +  L
Sbjct: 29  IVLLHGFPEFWYGWKNQIKPLVDAGYRVIAPDQRGYNLSDKPEGIDSYRIDTLRDDIIGL 88

Query: 169 WKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID-ASVYAEGTGNLATLPRLI-- 228
              +     +++G   G AVA   A   PE +++L+ I+    +   T      P+ +  
Sbjct: 89  ITQFTDEKAIVIGHDWGGAVAWHLASTRPEYLEKLIAINIPHPHVMKTVTPLYPPQWLKS 148

Query: 229 AYAGVFLLKSIP---LRL--YVNVLAFTGIS----LSTSLD-------WVNIGRLHCLLP 288
           +Y   F L  IP   LR   Y  +    G+S    L TS D       W   G L  +L 
Sbjct: 149 SYIAYFQLPDIPEASLRENDYDTLDKAIGLSDRPALFTSEDVSRYKEAWKQPGALTAMLN 208

Query: 289 WWEDATVSFMLSGGYNVSSQIEKVKQKTL---IIWGEDDQIISNKLAVRLHCELPNAFIR 348
           W+                S  EK   +T+   +IWG +D+ +S KLA       PN  + 
Sbjct: 209 WYR----------ALRKGSLAEKPSYETVPYRMIWGMEDRFLSRKLAKETERHCPNGHLI 268

Query: 349 QIPECGHLPHVEKPNFVAKLITQFV 351
            + E  H  + EKP  V +LI +++
Sbjct: 269 FVDEASHWINHEKPAIVNQLILEYL 283

BLAST of Carg09270 vs. TrEMBL
Match: tr|A0A0A0KGX4|A0A0A0KGX4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G366450 PE=4 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 3.7e-172
Identity = 297/337 (88.13%), Postives = 311/337 (92.28%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLS  +A++W SAP  +QWKKPKR GVILN+ PSFLPKEV NIKDQ AR LA+RI+RLPV
Sbjct: 1   MLSPTMASTWGSAPLHIQWKKPKRPGVILNELPSFLPKEVYNIKDQSARNLASRIQRLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSD C MSSCVKPSIQ+K+CPVVLLHGFDSSCLEWRYTYPLLEEAG ETWAVDILGWGF
Sbjct: 61  SFSDFCIMSSCVKPSIQNKDCPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDILGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIK+PMV+VGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKKPMVIVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPR IAYAGVFLLKSIPLR+YVNVL FTGI  STSLDW NIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRSIAYAGVFLLKSIPLRVYVNVLTFTGIPFSTSLDWANIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGY VSSQIEKVKQKTLIIWGEDDQIIS KL VRLHCELPNA IR 
Sbjct: 241 LPWWEDATVSFMLSGGYKVSSQIEKVKQKTLIIWGEDDQIISYKLGVRLHCELPNAVIRP 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVNN 364
           I ECGHLPHVEKPN VAKLITQFVHEDSRKEV +VN+
Sbjct: 301 IAECGHLPHVEKPNLVAKLITQFVHEDSRKEVQFVNS 337

BLAST of Carg09270 vs. TrEMBL
Match: tr|A0A1S3BDL9|A0A1S3BDL9_CUCME (uncharacterized hydrolase YugF OS=Cucumis melo OX=3656 GN=LOC103488473 PE=4 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 5.4e-171
Identity = 292/331 (88.22%), Postives = 306/331 (92.45%), Query Frame = 0

Query: 27  MLSLAVAASWCSAPPLMQWKKPKRLGVILNKFPSFLPKEVDNIKDQFARELATRIERLPV 86
           MLS  +AA+W SAP  +QWKKPKR+GV +N+FPSFLPKEV +IKDQFAR LA+RIERLPV
Sbjct: 1   MLSPTMAATWGSAPLHIQWKKPKRMGVTVNEFPSFLPKEVYDIKDQFARNLASRIERLPV 60

Query: 87  SFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILGWGF 146
           SFSDSC MSSCVKPSIQSK+CPVVLLHGFDSSCLEWRYTYPLLEEAG ETWAVD+LGWGF
Sbjct: 61  SFSDSCIMSSCVKPSIQSKKCPVVLLHGFDSSCLEWRYTYPLLEEAGLETWAVDVLGWGF 120

Query: 147 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLVLID 206
           SDLERLPPCDVTSKRVHLYQLWKSYIKRPMV+VGPSLGAAVAIDFAVNYPEAVDRLVLID
Sbjct: 121 SDLERLPPCDVTSKRVHLYQLWKSYIKRPMVIVGPSLGAAVAIDFAVNYPEAVDRLVLID 180

Query: 207 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRLHCL 266
           ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLR+YVN L FT I  ST LDW NIGRLHCL
Sbjct: 181 ASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRVYVNFLTFTSIPFSTGLDWANIGRLHCL 240

Query: 267 LPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAFIRQ 326
           LPWWEDATVSFMLSGGY VSSQIEKVKQKTLIIWGEDDQI+S KL VRLHCELPNA  R 
Sbjct: 241 LPWWEDATVSFMLSGGYKVSSQIEKVKQKTLIIWGEDDQIVSYKLGVRLHCELPNAVFRP 300

Query: 327 IPECGHLPHVEKPNFVAKLITQFVHEDSRKE 358
           I ECGHLPHVEKPN VAKLITQF+HEDSRKE
Sbjct: 301 IAECGHLPHVEKPNLVAKLITQFIHEDSRKE 331

BLAST of Carg09270 vs. TrEMBL
Match: tr|A0A2I4FP87|A0A2I4FP87_9ROSI (uncharacterized protein LOC109000893 isoform X1 OS=Juglans regia OX=51240 GN=LOC109000893 PE=4 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 4.4e-149
Identity = 261/339 (76.99%), Postives = 292/339 (86.14%), Query Frame = 0

Query: 30  LAVAASWCSAPPLMQWKK--PKRLGV--ILNKFPSFLPKEVDNIKDQFARELATRIERLP 89
           LA++     APPL   K+  PK LGV   L  FPSFLPKE++NIKD FAR LATRIERLP
Sbjct: 2   LALSPISAPAPPLKYTKRRAPKCLGVYSTLAGFPSFLPKEIENIKDPFARTLATRIERLP 61

Query: 90  --VSFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVDILG 149
             VSFSD+C MSSCV+P I+S+  PVVLLHGFDSSCLEWRYT+PLLEEAG E WAVDILG
Sbjct: 62  VSVSFSDNCIMSSCVRPLIRSETSPVVLLHGFDSSCLEWRYTHPLLEEAGLEAWAVDILG 121

Query: 150 WGFSDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVDRLV 209
           WGFSDLERLP CDVTSKR H YQLW SYIK+PM+LVGPSLGAAVAIDFAVN+PEAV++L+
Sbjct: 122 WGFSDLERLPSCDVTSKREHFYQLWNSYIKKPMILVGPSLGAAVAIDFAVNHPEAVEKLI 181

Query: 210 LIDASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNIGRL 269
           LIDASVYAEGTG+LA LPR++AYAGV+LLKSIPLRLY N L FTGIS STSLDW N+GRL
Sbjct: 182 LIDASVYAEGTGSLADLPRIVAYAGVYLLKSIPLRLYANFLTFTGISFSTSLDWTNVGRL 241

Query: 270 HCLLPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELPNAF 329
           HCLLPWWEDATV FM+SGGYNVSSQI++V QKTLIIWGE+DQI+SNKL VRLHCELPNA 
Sbjct: 242 HCLLPWWEDATVDFMISGGYNVSSQIKQVNQKTLIIWGENDQIVSNKLGVRLHCELPNAI 301

Query: 330 IRQIPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVN 363
           IRQ+PECGHLPHVEKPN VAKLI ++V ED  KE+  V+
Sbjct: 302 IRQLPECGHLPHVEKPNSVAKLILEYVQEDCYKEMECVH 340

BLAST of Carg09270 vs. TrEMBL
Match: tr|A0A2I4H872|A0A2I4H872_9ROSI (uncharacterized protein LOC109014370 isoform X1 OS=Juglans regia OX=51240 GN=LOC109014370 PE=4 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 5.8e-149
Identity = 259/342 (75.73%), Postives = 293/342 (85.67%), Query Frame = 0

Query: 25  TEMLSLAVAASWCSAPPLMQWKKPKRLGV--ILNKFPSFLPKEVDNIKDQFARELATRIE 84
           T+ML+L   ++    P   + + PK LGV   L  FPSFLPKE++NIKD FAR LATRIE
Sbjct: 80  TKMLALPPISAPAPPPKHTKRRAPKCLGVYSTLAGFPSFLPKEIENIKDPFARTLATRIE 139

Query: 85  RLP--VSFSDSCTMSSCVKPSIQSKECPVVLLHGFDSSCLEWRYTYPLLEEAGFETWAVD 144
           RLP  VSFSD+C MSSCV+P I+SK  PVVLLHGFDSSCLEWRYT+PLLEEAG E WAVD
Sbjct: 140 RLPVSVSFSDNCIMSSCVRPLIRSKTSPVVLLHGFDSSCLEWRYTHPLLEEAGLEAWAVD 199

Query: 145 ILGWGFSDLERLPPCDVTSKRVHLYQLWKSYIKRPMVLVGPSLGAAVAIDFAVNYPEAVD 204
           ILGWGFSDLERLP CDVTSKR H YQLW SYIK+PM+LVGPSLGAAVAIDFAVN+PEAV+
Sbjct: 200 ILGWGFSDLERLPRCDVTSKREHFYQLWNSYIKKPMILVGPSLGAAVAIDFAVNHPEAVE 259

Query: 205 RLVLIDASVYAEGTGNLATLPRLIAYAGVFLLKSIPLRLYVNVLAFTGISLSTSLDWVNI 264
           +L+LIDASVYAEGTG+LA LPR++AYAGV+LLKSIPLRLY N L FTGIS STSLDW N+
Sbjct: 260 KLILIDASVYAEGTGSLADLPRIVAYAGVYLLKSIPLRLYANYLTFTGISFSTSLDWTNV 319

Query: 265 GRLHCLLPWWEDATVSFMLSGGYNVSSQIEKVKQKTLIIWGEDDQIISNKLAVRLHCELP 324
           GRLHCLLPWWEDAT+ FM+SGGYNVSSQI++V QKTLIIWGE+DQI+SNKL VRLHCELP
Sbjct: 320 GRLHCLLPWWEDATLDFMISGGYNVSSQIKQVNQKTLIIWGENDQIVSNKLGVRLHCELP 379

Query: 325 NAFIRQIPECGHLPHVEKPNFVAKLITQFVHEDSRKEVPYVN 363
           NA IRQ+PECGHLPHVEKPN VAKLI ++V ED  KE+  V+
Sbjct: 380 NAIIRQLPECGHLPHVEKPNSVAKLILEYVQEDCYKEMECVH 421

BLAST of Carg09270 vs. TrEMBL
Match: tr|A0A2P4N806|A0A2P4N806_QUESU (Putative hydrolase yugf OS=Quercus suber OX=58331 GN=CFP56_55129 PE=4 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.7e-148
Identity = 254/302 (84.11%), Postives = 275/302 (91.06%), Query Frame = 0

Query: 58  FPSFLPKEVDNIKDQFARELATRIERL--PVSFSDSCTMSSCVKPSIQSKECPVVLLHGF 117
           FPSFLPK+V+NIKD FAR+LATRI+RL  PVSFSD+  MSSCV+P IQSK  PVVLLHGF
Sbjct: 17  FPSFLPKQVENIKDTFARKLATRIDRLPVPVSFSDNSIMSSCVRPLIQSKTSPVVLLHGF 76

Query: 118 DSSCLEWRYTYPLLEEAGFETWAVDILGWGFSDLERLPPCDVTSKRVHLYQLWKSYIKRP 177
           DSSCLEWRYTYPLLEEAG ETWAVDILGWGFSDLERLPPC+V SKR H YQLWKS+IKRP
Sbjct: 77  DSSCLEWRYTYPLLEEAGLETWAVDILGWGFSDLERLPPCNVASKREHFYQLWKSHIKRP 136

Query: 178 MVLVGPSLGAAVAIDFAVNYPEAVDRLVLIDASVYAEGTGNLATLPRLIAYAGVFLLKSI 237
           M+LVGPSLGAAVAIDF  N+PEAV++LVLIDASVYAEGTGNLATLPR+ AYAGV+LLKSI
Sbjct: 137 MILVGPSLGAAVAIDFVANHPEAVEKLVLIDASVYAEGTGNLATLPRIAAYAGVYLLKSI 196

Query: 238 PLRLYVNVLAFTGISLSTSLDWVNIGRLHCLLPWWEDATVSFMLSGGYNVSSQIEKVKQK 297
           PLRLYVNVLAFTGIS  TSLDW N+GRLHCL PWWEDATV FM+SGGYNVS+QI++VKQK
Sbjct: 197 PLRLYVNVLAFTGISFGTSLDWTNVGRLHCLYPWWEDATVDFMISGGYNVSAQIQQVKQK 256

Query: 298 TLIIWGEDDQIISNKLAVRLHCELPNAFIRQIPECGHLPHVEKPNFVAKLITQFVHEDSR 357
           TLIIWGEDDQIISNKLAVRLHCELPNA IRQIP+CGHLPHVE+PN VAK I  FVHED  
Sbjct: 257 TLIIWGEDDQIISNKLAVRLHCELPNATIRQIPDCGHLPHVERPNSVAKFIVDFVHEDCY 316

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022962117.11.3e-197100.00uncharacterized protein LOC111462669 isoform X1 [Cucurbita moschata][more]
XP_022962121.11.5e-193100.00uncharacterized protein LOC111462669 isoform X3 [Cucurbita moschata][more]
XP_022962120.13.4e-19399.70uncharacterized protein LOC111462669 isoform X2 [Cucurbita moschata][more]
XP_022997199.12.2e-19299.10uncharacterized protein LOC111492171 isoform X2 [Cucurbita maxima][more]
XP_022997198.14.9e-19298.80uncharacterized protein LOC111492171 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G13820.13.7e-13373.48alpha/beta-Hydrolases superfamily protein[more]
AT5G39220.12.0e-11561.71alpha/beta-Hydrolases superfamily protein[more]
AT4G36530.26.3e-1625.09alpha/beta-Hydrolases superfamily protein[more]
AT5G09430.13.8e-1327.47alpha/beta-Hydrolases superfamily protein[more]
AT1G78210.15.5e-1224.28alpha/beta-Hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
sp|O05235|YUGF_BACSU3.9e-1524.80Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yu... [more]
sp|A4JPX5|MHPC_BURVG8.9e-1225.682-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Burk... [more]
sp|Q476M7|MHPC_CUPNJ8.9e-1226.922-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Cupr... [more]
sp|B2JQW2|MHPC_PARP81.2e-1125.492-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase OS=Para... [more]
sp|O31581|YFHM_BACSU2.6e-1126.79AB hydrolase superfamily protein YfhM OS=Bacillus subtilis (strain 168) OX=22430... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0KGX4|A0A0A0KGX4_CUCSA3.7e-17288.13Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G366450 PE=4 SV=1[more]
tr|A0A1S3BDL9|A0A1S3BDL9_CUCME5.4e-17188.22uncharacterized hydrolase YugF OS=Cucumis melo OX=3656 GN=LOC103488473 PE=4 SV=1[more]
tr|A0A2I4FP87|A0A2I4FP87_9ROSI4.4e-14976.99uncharacterized protein LOC109000893 isoform X1 OS=Juglans regia OX=51240 GN=LOC... [more]
tr|A0A2I4H872|A0A2I4H872_9ROSI5.8e-14975.73uncharacterized protein LOC109014370 isoform X1 OS=Juglans regia OX=51240 GN=LOC... [more]
tr|A0A2P4N806|A0A2P4N806_QUESU1.7e-14884.11Putative hydrolase yugf OS=Quercus suber OX=58331 GN=CFP56_55129 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: INTERPRO
TermDefinition
IPR029058AB_hydrolase
IPR000073AB_hydrolase_1
IPR000639Epox_hydrolase-like
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09270-RACarg09270-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 133..148
score: 39.2
coord: 192..205
score: 29.22
coord: 327..349
score: 27.67
coord: 113..131
score: 36.84
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 178..191
score: 42.86
coord: 296..310
score: 41.84
coord: 192..205
score: 43.35
coord: 133..148
score: 35.13
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 109..344
e-value: 2.2E-15
score: 57.8
IPR029058Alpha/Beta hydrolase foldGENE3DG3DSA:3.40.50.1820coord: 101..359
e-value: 1.5E-42
score: 147.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILYSSF53474alpha/beta-Hydrolasescoord: 107..350
NoneNo IPR availablePANTHERPTHR43689FAMILY NOT NAMEDcoord: 54..358
NoneNo IPR availablePANTHERPTHR43689:SF3ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 54..358