Carg08708 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg08708
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Bifunctional nuclease 2
Location	Cucurbita_argyrosperma_scaffold_057 : 600962 .. 604302 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATTCACACTCCTCCATTGAAGAATCCATTTATCTTCTTCCCCGTTTGCATTTCCTCCTTATCCGTTCACCTTGTCGGTTTTTTCCTTCCGATTTTGTTCTTTCAATTATCTGTCAATTTCCTTCTTCGATCTGTGTCGTTGGTAATTTGTTAACTTAAATTTAGCCATCGATTCCGTTCTTGAAATTTAGATTTTTCATTCTAGAATTTCGGGATGCGGGAATTGTTTTACCGGATCGAGCTGTGATCGCCGTTTGTAGCGTACTAATCGGAGAGCTCTGTTACGAGTTTTTGACGTGATACGGCCTACTTTTCGTATAAAAGGTAACGGATCTTCAAACACTTCTCAGTGCTAAGACTGATGATCAAGACTCGCTTTATTTCCCACCGACGATAACGGTAGATATCTCTGATAGGTAATGGAAAACGTTGAATCCGACTGTAATTCATTCGAACTGATGGCTGAGATTGTTTTAATTTTCTTTGCTCGAATTTCTTCACTTCAAATTTCAGTTTCCTTTCTGTAGCGTTCACCAATTATATGCGTTCGAATCGCCAATTTCTATTTCTGTATTTTTGGTCAGGATTGGGGATTTTGCACATTAGTTTTATCTGATCATTCACATTTGGGTGAACTGAAGGACGCGAGATGGGGTCTTTGCAAGGACCGGTTGTTTGCCCAACTATTCGCGCCAAGCAAGCAGGATTCTGTTCTCTGCCAATCAATCGTTCTTTAATGGATTTTAGGTTTCGAAGAGGTGAATTGTGGGGTTTTGCTGGGACCAACGGTTTGCGTGCCAAGTTACCAGGTATTTCTCAGCAAGGCTGTGCTAGAAAATGCAAGACATTGTGTTGCAGCTTTAATTCATCATCGGACGACAATGGAAGTACAGCAGAGAACTTCAACGGGAAGGATGAGGAATACGTTGAATCGAGCGTGATTGAGGCTGGTATGCTGAGTTGATGGCCTAATTCATCATCATTCGTTTTGGTTTTATGGTGCTATTACCTAATTGAAATGGCTGTTGGTTTGTTTGTGCTGTATAGTTGAGGTGAAAAGTGGTGCCGATGAATTCCTGATTAAAATGAGGGATGGTAGGCACTTAAGGTGTGTTCACAACAACCCTCACGGTGGGCATCTGCCTGATTATGCTCCACATCCTGCAATTGTGTTGAAGATGGAAGATGGGACTGGTCTTCTTCTTCCCATAATCGTGTGTAAGTATCGTTCGATTATACTGTCTAACGCATGCTCTTAGTGTTTTTGAACGAATTCGAACTTTTTTTGCCAACATTTCATCTTTTGCAGTGGAAATGCCAAGCGTGCTACTTATGGCTGCTATGCGAAATGTTCCAATTGTATGTTTGCATTGTTTCCTCTTGAGATTGGATATAACATTTTGGAAATCGAATTATATGCATTTGGAAATGAATTTCTAACAGTTATTATTATTTGGTTCCAGGCCAGACCAACGATGTATCAAGTGGTGAAAGAGATGATTGAAAAGATGGGTTATGAAGTATGCTTTGATTCATCTTTCTCTTTTTCCACCTTGTCTTTGAGTTTAAAGCTTAAGTGGGATGTGGGATGTGGACTGTTTGTAATTTTAGTCGCGAATATTTTATTTATCGATGCAGGTCAAACTCGTAAGGGTTACGAAGAGAGTGCACGAGGCATATTTTGCTCAGTTATATCTCTCAAAGGCATGTATAAGTTTCTCCCTCTGGATTCTGTACCCTTTTCTTAGATAAGTTTAAGAGATTTGAGTGTTGTGCGTTTCAGATTGGTTGTGAGACTGACTGCATAAGCTTCGATCTCCGACCTTCAGATGCAATAAACATTGCAGTCCGATGCAAGGTAACTGAAACTGTTTCATGCTTACTTTTCCTGGTTGAGTGTAGCGACTCGGGTCAAGTGCCTCTTTGCGTGGCCGAAAGAATTGTTGAAATATTTCAGTTAGTATGTGGCGTTGAATTGCTGAAATATTTAGATGTTTTTCAAATATTAGGCATCTTGCAATTGATGCAGGTCCCAATTCAAGTGAACAAGTATCTTGCCTACAGTGATGGCATGAAAGTTGTTGAAACTGGGAAGCTGCCATCCCAGGCCCCTGCTACGGATGGCTTATTATTCACCGAAGTGGATAGGTAATAAAATATTTTGACAATTTGAGACTCCTGGTTCATTTGACAAGAGAAAATGGGATTTAATTGAATTCTTTTGTCTTCAGGCCTAATGGTCAGCCTTGTGCTGAAGCCAAAGAGTTCAATCTTATCAGGAATATGCTGATTGCTGCTGTTGAAGAGCGCTACAGAGATGCTGGTACCTATTCTGTTGTTCATTTATGAGCGTTCTAAATGAATTTTACTTCTTTGATTTAGAACTGAAGCTTCCATTCTTTAGTAATGTTTTACCAGAGAGTCGTTGGTGTTCATGTGAAAGGTCGAAAGACCAAGAATAGTTAATTGCTTAATATGCGATGACCTTGTGGGTTGTACTTTTCCAAACACAAACAAAATGATATGAGAAAGGGAGGGTCGAGAGAGTCAACTAATTGGCGGGCTTGAGGAATAGTCGAAGCCATACCCAATCGAAAAAGGATGTTATCCAAACTTGACCCGTTTTTGGCTTTTCCAGTGATAGAAACTTATTTATTCAAGTAATTGTCGTTCTGTTTTAGATAGTTCTAAAAAATCATCATAGGTATCCATTTGACAAGTGCAATTTTAGGACAAAAACAAGCCCCATTTTGCCAATGATGTGAGATTATATTCAGCGTGTGAGCTGTACAATATACATTTGGTTCTCATTGTTGGTCTGTATTTCAGCTCAATGGAGGGACAAGCTCAATCTTCTGAGGGCTAGGAGGAATTTGGCGTAAGAGGTAGACAAACTCCTTCATTCTCTATATTCAATATTTCTATCACACTTTTTTGGTTGAATCAAGAGGATGTAGACTGAAAAATACTCTCTGTTTCTTCAGTTACTCCTGGAGAAAGAATACATGTACATAGAACGTAATTGTTCGGGGCCGAGGTTGGTTTGCGTGGGTAAATTACATTGCCTCTCGCCTCTTTCTACTTGCGTTATGTATTTAGCAAACTCTGTGAATATACGTATAGATATCTATATATATGTGTGTGTGTGTTTGTAGATAGAAGAACTTGGTGGTTTCAGGTCGTCAGATGTGTTCTTTGTGAAATGCATGATATAACCAAATCCTTTGATGCTTCACAATCACGAATTGCTTATTTATATGTTAGTCACTGCTTACATAATTTGATAGCCAAGAGTCCCGTTTTCATTCTAGTAGCTCGCCTAAATTTAGAACTTTAAAATA

mRNA sequence

CATTCACACTCCTCCATTGAAGAATCCATTTATCTTCTTCCCCGTTTGCATTTCCTCCTTATCCGTTCACCTTGTCGGTTTTTTCCTTCCGATTTTGTTCTTTCAATTATCTGTCAATTTCCTTCTTCGATCTGTGTCGTTGGTAATTTGTTAACTTAAATTTAGCCATCGATTCCGTTCTTGAAATTTAGATTTTTCATTCTAGAATTTCGGGATGCGGGAATTGTTTTACCGGATCGAGCTGTGATCGCCGTTTGTAGCGTACTAATCGGAGAGCTCTGTTACGAGTTTTTGACGTGATACGGCCTACTTTTCGTATAAAAGGTAACGGATCTTCAAACACTTCTCAGTGCTAAGACTGATGATCAAGACTCGCTTTATTTCCCACCGACGATAACGGTAGATATCTCTGATAGGTAATGGAAAACGTTGAATCCGACTGTAATTCATTCGAACTGATGGCTGAGATTGTTTTAATTTTCTTTGCTCGAATTTCTTCACTTCAAATTTCAGTTTCCTTTCTGTAGCGTTCACCAATTATATGCGTTCGAATCGCCAATTTCTATTTCTGTATTTTTGGTCAGGATTGGGGATTTTGCACATTAGTTTTATCTGATCATTCACATTTGGGTGAACTGAAGGACGCGAGATGGGGTCTTTGCAAGGACCGGTTGTTTGCCCAACTATTCGCGCCAAGCAAGCAGGATTCTGTTCTCTGCCAATCAATCGTTCTTTAATGGATTTTAGGTTTCGAAGAGGTGAATTGTGGGGTTTTGCTGGGACCAACGGTTTGCGTGCCAAGTTACCAGGTATTTCTCAGCAAGGCTGTGCTAGAAAATGCAAGACATTGTGTTGCAGCTTTAATTCATCATCGGACGACAATGGAAGTACAGCAGAGAACTTCAACGGGAAGGATGAGGAATACGTTGAATCGAGCGTGATTGAGGCTGTTGAGGTGAAAAGTGGTGCCGATGAATTCCTGATTAAAATGAGGGATGGTAGGCACTTAAGGTGTGTTCACAACAACCCTCACGGTGGGCATCTGCCTGATTATGCTCCACATCCTGCAATTGTGTTGAAGATGGAAGATGGGACTGGTCTTCTTCTTCCCATAATCGTGTTGGAAATGCCAAGCGTGCTACTTATGGCTGCTATGCGAAATGTTCCAATTGCCAGACCAACGATGTATCAAGTGGTGAAAGAGATGATTGAAAAGATGGGTTATGAAATTGGTTGTGAGACTGACTGCATAAGCTTCGATCTCCGACCTTCAGATGCAATAAACATTGCAGTCCGATGCAAGGTCCCAATTCAAGTGAACAAGTATCTTGCCTACAGTGATGGCATGAAAGTTGTTGAAACTGGGAAGCTGCCATCCCAGGCCCCTGCTACGGATGGCTTATTATTCACCGAAGTGGATAGGCCTAATGGTCAGCCTTGTGCTGAAGCCAAAGAGTTCAATCTTATCAGGAATATGCTGATTGCTGCTGTTGAAGAGCGCTACAGAGATGCTGCTCAATGGAGGGACAAGCTCAATCTTCTGAGGGCTAGGAGGAATTTGGCGTAAGAGTTACTCCTGGAGAAAGAATACATGTACATAGAACGTAATTGTTCGGGGCCGAGGTTGGTTTGCGTGGGTAAATTACATTGCCTCTCGCCTCTTTCTACTTGCGTTATGTATTTAGCAAACTCTGTGAATATACGTATAGATATCTATATATATGTGTGTGTGTGTTTGTAGATAGAAGAACTTGGTGGTTTCAGGTCGTCAGATGTGTTCTTTGTGAAATGCATGATATAACCAAATCCTTTGATGCTTCACAATCACGAATTGCTTATTTATATGTTAGTCACTGCTTACATAATTTGATAGCCAAGAGTCCCGTTTTCATTCTAGTAGCTCGCCTAAATTTAGAACTTTAAAATA

Coding sequence (CDS)

ATGGGGTCTTTGCAAGGACCGGTTGTTTGCCCAACTATTCGCGCCAAGCAAGCAGGATTCTGTTCTCTGCCAATCAATCGTTCTTTAATGGATTTTAGGTTTCGAAGAGGTGAATTGTGGGGTTTTGCTGGGACCAACGGTTTGCGTGCCAAGTTACCAGGTATTTCTCAGCAAGGCTGTGCTAGAAAATGCAAGACATTGTGTTGCAGCTTTAATTCATCATCGGACGACAATGGAAGTACAGCAGAGAACTTCAACGGGAAGGATGAGGAATACGTTGAATCGAGCGTGATTGAGGCTGTTGAGGTGAAAAGTGGTGCCGATGAATTCCTGATTAAAATGAGGGATGGTAGGCACTTAAGGTGTGTTCACAACAACCCTCACGGTGGGCATCTGCCTGATTATGCTCCACATCCTGCAATTGTGTTGAAGATGGAAGATGGGACTGGTCTTCTTCTTCCCATAATCGTGTTGGAAATGCCAAGCGTGCTACTTATGGCTGCTATGCGAAATGTTCCAATTGCCAGACCAACGATGTATCAAGTGGTGAAAGAGATGATTGAAAAGATGGGTTATGAAATTGGTTGTGAGACTGACTGCATAAGCTTCGATCTCCGACCTTCAGATGCAATAAACATTGCAGTCCGATGCAAGGTCCCAATTCAAGTGAACAAGTATCTTGCCTACAGTGATGGCATGAAAGTTGTTGAAACTGGGAAGCTGCCATCCCAGGCCCCTGCTACGGATGGCTTATTATTCACCGAAGTGGATAGGCCTAATGGTCAGCCTTGTGCTGAAGCCAAAGAGTTCAATCTTATCAGGAATATGCTGATTGCTGCTGTTGAAGAGCGCTACAGAGATGCTGCTCAATGGAGGGACAAGCTCAATCTTCTGAGGGCTAGGAGGAATTTGGCGTAA

Protein sequence

MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGCARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHLRCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMYQVVKEMIEKMGYEIGCETDCISFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLIAAVEERYRDAAQWRDKLNLLRARRNLA

Homology

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BLAST of Carg08708 vs. NCBI nr
Match: XP_022937113.1 (bifunctional nuclease 1-like [Cucurbita moschata] >XP_022937114.1 bifunctional nuclease 1-like [Cucurbita moschata] >XP_022937115.1 bifunctional nuclease 1-like [Cucurbita moschata] >XP_022937117.1 bifunctional nuclease 1-like [Cucurbita moschata])

HSP 1 Score: 605.9 bits (1561), Expect = 7.5e-170
Identity = 304/327 (92.97%), Postives = 305/327 (93.27%), Query Frame = 0


Query: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC 60
           MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC
Sbjct: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC 60

Query: 61  ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHL 120
           ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHL
Sbjct: 61  ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHL 120

Query: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY 180
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY 180

Query: 181 QVVKEMIEKMGYE----------------------IGCETDCISFDLRPSDAINIAVRCK 240
           QVVKEMIEKMGYE                      IGCETDCISFDLRPSDAINIAVRCK
Sbjct: 181 QVVKEMIEKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCETDCISFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLI 300
           VPIQVNKYLAYSDGM+VVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLI
Sbjct: 241 VPIQVNKYLAYSDGMRVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLI 300

Query: 301 AAVEERYRDAAQWRDKLNLLRARRNLA 306
           AAVEERYRDAAQWRDKLNLLRARRNLA
Sbjct: 301 AAVEERYRDAAQWRDKLNLLRARRNLA 327

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BLAST of Carg08708 vs. NCBI nr
Match: XP_023536147.1 (bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023536148.1 bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023536149.1 bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023536150.1 bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 601.3 bits (1549), Expect = 1.9e-168
Identity = 301/327 (92.05%), Postives = 304/327 (92.97%), Query Frame = 0


Query: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC 60
           MGSLQGPVVCPTIRAKQAGFCSLPINRSLM FRFRRGELWGFAGTNGLRAKLPGISQQGC
Sbjct: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMAFRFRRGELWGFAGTNGLRAKLPGISQQGC 60

Query: 61  ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHL 120
           ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEF+IKMRDGRHL
Sbjct: 61  ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFVIKMRDGRHL 120

Query: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY 180
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY 180

Query: 181 QVVKEMIEKMGYE----------------------IGCETDCISFDLRPSDAINIAVRCK 240
           QVVKEMIEKMGYE                      IGCETDCISFDLRPSDAINIAVRCK
Sbjct: 181 QVVKEMIEKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCETDCISFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLI 300
           VPIQVNKYLAYSDGM+VVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNL+RNMLI
Sbjct: 241 VPIQVNKYLAYSDGMRVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLVRNMLI 300

Query: 301 AAVEERYRDAAQWRDKLNLLRARRNLA 306
           AAVEERYRDAAQWRDKLNLLRARRNLA
Sbjct: 301 AAVEERYRDAAQWRDKLNLLRARRNLA 327

[Close]

BLAST of Carg08708 vs. NCBI nr
Match: XP_022975541.1 (bifunctional nuclease 1-like [Cucurbita maxima] >XP_022975542.1 bifunctional nuclease 1-like [Cucurbita maxima] >XP_022975544.1 bifunctional nuclease 1-like [Cucurbita maxima] >XP_022975553.1 bifunctional nuclease 1-like [Cucurbita maxima])

HSP 1 Score: 593.6 bits (1529), Expect = 3.9e-166
Identity = 298/327 (91.13%), Postives = 302/327 (92.35%), Query Frame = 0


Query: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC 60
           MGSLQGPVVCPT+RAKQAGFC+LPINRSLM FRFRRGELWGFAGTNGLRAKLPGISQQGC
Sbjct: 1   MGSLQGPVVCPTMRAKQAGFCTLPINRSLMAFRFRRGELWGFAGTNGLRAKLPGISQQGC 60

Query: 61  ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHL 120
           ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEV S ADEF+IKMRDGRHL
Sbjct: 61  ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVTSVADEFVIKMRDGRHL 120

Query: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY 180
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY 180

Query: 181 QVVKEMIEKMGYE----------------------IGCETDCISFDLRPSDAINIAVRCK 240
           QVVKEMIEKMGYE                      IGCETDCISFDLRPSDAINIAVRCK
Sbjct: 181 QVVKEMIEKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCETDCISFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLI 300
           VPIQVNKYLAYSDGM+VVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLI
Sbjct: 241 VPIQVNKYLAYSDGMRVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLI 300

Query: 301 AAVEERYRDAAQWRDKLNLLRARRNLA 306
           AAVEERYRDAAQWRDKLNLLRARRNLA
Sbjct: 301 AAVEERYRDAAQWRDKLNLLRARRNLA 327

[Close]

BLAST of Carg08708 vs. NCBI nr
Match: XP_008466529.1 (PREDICTED: bifunctional nuclease 1 [Cucumis melo] >XP_008466530.1 PREDICTED: bifunctional nuclease 1 [Cucumis melo])

HSP 1 Score: 559.7 bits (1441), Expect = 6.2e-156
Identity = 279/327 (85.32%), Postives = 293/327 (89.60%), Query Frame = 0


Query: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC 60
           MGSLQGPVVCPTIRAKQAGFCSLPINRSLM F FRRGELWGF G NGL AKLP IS +GC
Sbjct: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMAFSFRRGELWGFTGINGLNAKLPAISLKGC 60

Query: 61  ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHL 120
           ARKCKTL CSF+SSSDDNGSTAENFN KDEEYVESSV+EAVEVKSGAD F+IKMRDGRHL
Sbjct: 61  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 120

Query: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY 180
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAA+RNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 181 QVVKEMIEKMGYE----------------------IGCETDCISFDLRPSDAINIAVRCK 240
           QVVKEM++KMGYE                      IGCETDC+SFDLRPSDAINIAVRCK
Sbjct: 181 QVVKEMVDKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCETDCLSFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLI 300
           VPIQVNKYLAYSDGM+VVE+GKL +QAPATDGLLFTE+DRP+GQPC EAKEFNL+RNMLI
Sbjct: 241 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 300

Query: 301 AAVEERYRDAAQWRDKLNLLRARRNLA 306
           AAVEERYRDAAQWRDKLNLLRARRNLA
Sbjct: 301 AAVEERYRDAAQWRDKLNLLRARRNLA 327

[Close]

BLAST of Carg08708 vs. NCBI nr
Match: XP_023525215.1 (bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023525216.1 bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023525218.1 bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023525219.1 bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 547.0 bits (1408), Expect = 4.1e-152
Identity = 271/327 (82.87%), Postives = 288/327 (88.07%), Query Frame = 0


Query: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC 60
           MGSLQGPVVCPT RAKQAGFCSLPI RSLM F FRRGE WGF G NGL AK P IS++GC
Sbjct: 1   MGSLQGPVVCPTFRAKQAGFCSLPIKRSLMAFSFRRGEFWGFTGINGLNAKFPAISRKGC 60

Query: 61  ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHL 120
           ARKC T+ CSF+SSSDDNGSTAENFN KDEEYVESSV+EAVEVKSGAD F+IKMRDGRHL
Sbjct: 61  ARKCTTVRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFMIKMRDGRHL 120

Query: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY 180
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAA+RNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 181 QVVKEMIEKMGYE----------------------IGCETDCISFDLRPSDAINIAVRCK 240
           QVVKEMI+KMGYE                      IGC+TDC+SFDLRPSDAINIAVRCK
Sbjct: 181 QVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCDTDCLSFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLI 300
           VPIQVNKYLAYSDGM+VVETGKL +Q PATDGLLFTE+DRP+GQPC EAKEF+L+RNMLI
Sbjct: 241 VPIQVNKYLAYSDGMRVVETGKLSTQTPATDGLLFTELDRPSGQPCVEAKEFDLVRNMLI 300

Query: 301 AAVEERYRDAAQWRDKLNLLRARRNLA 306
           AAVEERYRDAAQWRDKLNLLRAR+NLA
Sbjct: 301 AAVEERYRDAAQWRDKLNLLRARKNLA 327

[Close]

BLAST of Carg08708 vs. TAIR10
Match: AT1G75380.1 (bifunctional nuclease in basal defense response 1)

HSP 1 Score: 397.5 bits (1020), Expect = 7.4e-111
Identity = 207/327 (63.30%), Postives = 245/327 (74.92%), Query Frame = 0


Query: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC 60
           M S+Q PVVCP IR +Q G C+  +N + +     R + WG   T G++++  G +    
Sbjct: 1   MRSVQAPVVCPAIRPRQVGACASLVNYTGLK---PRSQFWG-NRTKGVKSQ--GTTTTIT 60

Query: 61  ARKC-KTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRH 120
            R C K++ C F+S SD NGSTAENFN  DEEYV SSV+EAVEVKSGAD F++KMRDGR 
Sbjct: 61  LRLCNKSIKCVFSSHSDGNGSTAENFNENDEEYVNSSVVEAVEVKSGADGFMVKMRDGRQ 120

Query: 121 LRCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTM 180
           LRCVHNNP GGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAM NV IARPTM
Sbjct: 121 LRCVHNNPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARPTM 180

Query: 181 YQVVKEMIEKMGYE----------------------IGCETDCISFDLRPSDAINIAVRC 240
           YQVVKEM++KMGYE                      +G  ++C+SFDLRPSDAINIAVRC
Sbjct: 181 YQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAVRC 240

Query: 241 KVPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNML 300
           K+PIQVNKYLAYSDGM+V+E+GK+ + APA+DGLLFTE DRPNGQ C + KEFN++  M+
Sbjct: 241 KIPIQVNKYLAYSDGMRVIESGKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSKMM 300

Query: 301 IAAVEERYRDAAQWRDKLNLLRARRNL 305
            A  EERY +AA+WRDKL   RA+RNL
Sbjct: 301 QAVDEERYDEAAEWRDKLGQFRAKRNL 321

[Close]

BLAST of Carg08708 vs. TAIR10
Match: AT1G19660.1 (Wound-responsive family protein)

HSP 1 Score: 395.6 bits (1015), Expect = 2.8e-110
Identity = 201/326 (61.66%), Postives = 247/326 (75.77%), Query Frame = 0


Query: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC 60
           M SLQ PVVCP++R +Q G  +L +N S+   R  R + WG    N  +++   ++ +  
Sbjct: 1   MRSLQAPVVCPSVRPRQLGVSALLVNCSVSKTRSLRKQFWGNQTKND-KSQAATVNLRLH 60

Query: 61  ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHL 120
            R+ K++ C F+S SD  GSTAENFN  DE+YV+SSV+EAVEVKSG D F++KM+DGR L
Sbjct: 61  LRRYKSIKCLFSSHSDGTGSTAENFNENDEDYVKSSVLEAVEVKSGPDGFMVKMKDGRQL 120

Query: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY 180
           RCVHNNP GG+LP+YAPH AIVLKMEDGTGLLLPIIVLEMPSVLLMAAM NV IARPTMY
Sbjct: 121 RCVHNNPQGGNLPNYAPHSAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARPTMY 180

Query: 181 QVVKEMIEKMGYE----------------------IGCETDCISFDLRPSDAINIAVRCK 240
           QVVK+M++KMGYE                      +G ++DC+SFDLRPSDAINIAVRCK
Sbjct: 181 QVVKDMVDKMGYEVRLVRVTTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLI 300
           VPIQVNKYLAYSDGM+V+++GKL  Q PA+DGLLFTE+DRPNGQPC + KEF+L+RNM+ 
Sbjct: 241 VPIQVNKYLAYSDGMRVIDSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQ 300

Query: 301 AAVEERYRDAAQWRDKLNLLRARRNL 305
           A  EERY +AA+WRDKL   +A+R L
Sbjct: 301 AVDEERYDEAAEWRDKLGKFQAKRKL 325

[Close]

BLAST of Carg08708 vs. TAIR10
Match: AT5G66050.1 (Wound-responsive family protein)

HSP 1 Score: 88.6 bits (218), Expect = 7.3e-18
Identity = 61/183 (33.33%), Postives = 91/183 (49.73%), Query Frame = 0


Query: 139 PAIVLKMEDGTGLLLPIIVLEMP-SVLLMAAMRNVPIARPTMYQVVKEMIEKMGYEIGC- 198
           P I LK+      LLP+IV +     LL   ++      P  +Q V  +++K+GYE+   
Sbjct: 144 PTIFLKISCDGDYLLPVIVGDAAVEKLLDVPLQGHTEECPDQFQFVSAVVDKLGYEVKMV 203

Query: 199 --------------------ETDCISFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMKVV 258
                               + + I  D RPSDAIN+A  C+ PI VNK +   + +K+ 
Sbjct: 204 KLTGRIVNTYYASLCLGKPGDIEAICIDSRPSDAINVARACQAPIYVNKAIVLEEAIKIG 263

Query: 259 ETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLIAAVEERYRDAAQWRDKLN 300
             G+  S  P  + +L +  D P+  P +E  E  L+RNM +A+ EERY DAA WRD+L 
Sbjct: 264 YGGRPQSAKPVFNVILDSAPDGPD--PLSE--ELKLVRNMDLASKEERYIDAAMWRDRLK 322

[Close]

BLAST of Carg08708 vs. Swiss-Prot
Match: sp|Q9FWS6|BBD1_ARATH (Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 1.3e-109
Identity = 207/327 (63.30%), Postives = 245/327 (74.92%), Query Frame = 0


Query: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC 60
           M S+Q PVVCP IR +Q G C+  +N + +     R + WG   T G++++  G +    
Sbjct: 1   MRSVQAPVVCPAIRPRQVGACASLVNYTGLK---PRSQFWG-NRTKGVKSQ--GTTTTIT 60

Query: 61  ARKC-KTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRH 120
            R C K++ C F+S SD NGSTAENFN  DEEYV SSV+EAVEVKSGAD F++KMRDGR 
Sbjct: 61  LRLCNKSIKCVFSSHSDGNGSTAENFNENDEEYVNSSVVEAVEVKSGADGFMVKMRDGRQ 120

Query: 121 LRCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTM 180
           LRCVHNNP GGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAM NV IARPTM
Sbjct: 121 LRCVHNNPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARPTM 180

Query: 181 YQVVKEMIEKMGYE----------------------IGCETDCISFDLRPSDAINIAVRC 240
           YQVVKEM++KMGYE                      +G  ++C+SFDLRPSDAINIAVRC
Sbjct: 181 YQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAVRC 240

Query: 241 KVPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNML 300
           K+PIQVNKYLAYSDGM+V+E+GK+ + APA+DGLLFTE DRPNGQ C + KEFN++  M+
Sbjct: 241 KIPIQVNKYLAYSDGMRVIESGKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSKMM 300

Query: 301 IAAVEERYRDAAQWRDKLNLLRARRNL 305
            A  EERY +AA+WRDKL   RA+RNL
Sbjct: 301 QAVDEERYDEAAEWRDKLGQFRAKRNL 321

[Close]

BLAST of Carg08708 vs. Swiss-Prot
Match: sp|Q93VH2|BBD2_ARATH (Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 5.0e-109
Identity = 201/326 (61.66%), Postives = 247/326 (75.77%), Query Frame = 0


Query: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC 60
           M SLQ PVVCP++R +Q G  +L +N S+   R  R + WG    N  +++   ++ +  
Sbjct: 1   MRSLQAPVVCPSVRPRQLGVSALLVNCSVSKTRSLRKQFWGNQTKND-KSQAATVNLRLH 60

Query: 61  ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHL 120
            R+ K++ C F+S SD  GSTAENFN  DE+YV+SSV+EAVEVKSG D F++KM+DGR L
Sbjct: 61  LRRYKSIKCLFSSHSDGTGSTAENFNENDEDYVKSSVLEAVEVKSGPDGFMVKMKDGRQL 120

Query: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY 180
           RCVHNNP GG+LP+YAPH AIVLKMEDGTGLLLPIIVLEMPSVLLMAAM NV IARPTMY
Sbjct: 121 RCVHNNPQGGNLPNYAPHSAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARPTMY 180

Query: 181 QVVKEMIEKMGYE----------------------IGCETDCISFDLRPSDAINIAVRCK 240
           QVVK+M++KMGYE                      +G ++DC+SFDLRPSDAINIAVRCK
Sbjct: 181 QVVKDMVDKMGYEVRLVRVTTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLI 300
           VPIQVNKYLAYSDGM+V+++GKL  Q PA+DGLLFTE+DRPNGQPC + KEF+L+RNM+ 
Sbjct: 241 VPIQVNKYLAYSDGMRVIDSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQ 300

Query: 301 AAVEERYRDAAQWRDKLNLLRARRNL 305
           A  EERY +AA+WRDKL   +A+R L
Sbjct: 301 AVDEERYDEAAEWRDKLGKFQAKRKL 325

[Close]

BLAST of Carg08708 vs. Swiss-Prot
Match: sp|Q6YZM6|BBD2_ORYSJ (Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 1.4e-87
Identity = 183/330 (55.45%), Postives = 232/330 (70.30%), Query Frame = 0


Query: 3   SLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTN--GLRAKLPGISQ--- 62
           +++GP++C  +   QA      I+ SL     + G+L    GT   G   K   I++   
Sbjct: 2   AMEGPILCRAV--MQAKLPVTMISNSLT----KSGQL----GTAFLGCVCKYRNITRLIS 61

Query: 63  ---QGCARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKM 122
              Q   +   T+C SF+SSSD NG  A NF+  DE+YV S+V+EAVEV+SGA+ ++IKM
Sbjct: 62  PIYQPAQKNFATVCGSFSSSSDGNGYMAGNFSESDEDYVNSTVLEAVEVRSGAEGYVIKM 121

Query: 123 RDGRHLRCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPI 182
           RDG++LRCVHNN  G ++P+ AP PAIVL++EDG+  LLPIIVLEMPSVLLMAA+RNV I
Sbjct: 122 RDGKNLRCVHNNSQGRNIPESAPQPAIVLRIEDGSETLLPIIVLEMPSVLLMAAIRNVHI 181

Query: 183 ARPTMYQVVKEMIEKMGYE----------------------IGCETDCISFDLRPSDAIN 242
           ARPT+YQVVKEMI+KMGYE                      +G  T+ I+FDLRPSDAIN
Sbjct: 182 ARPTIYQVVKEMIDKMGYEVKLVRINKRIQEAYCAELFLTKVGDHTESITFDLRPSDAIN 241

Query: 243 IAVRCKVPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNL 302
           IAVRCKVPIQV++ LAYSDG++ VE  ++   A  +DGLLFTE+DRP+GQPC EA+EF L
Sbjct: 242 IAVRCKVPIQVHRSLAYSDGIRSVEPARMAIAAGMSDGLLFTELDRPDGQPCVEAQEFGL 301

[Close]

BLAST of Carg08708 vs. Swiss-Prot
Match: sp|Q5N8J3|BBD1_ORYSJ (Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 2.9e-72
Identity = 143/259 (55.21%), Postives = 193/259 (74.52%), Query Frame = 0


Query: 67  LCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHLRCVHNN 126
           +CCS+ SSSD +G+ A +++   EE+V SSV+EAVE++S +D F+IKMRDG++LRCV NN
Sbjct: 71  VCCSYGSSSDGDGAAAADYDASGEEFVNSSVMEAVELRSVSDGFVIKMRDGKNLRCVQNN 130

Query: 127 PHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMYQVVKEM 186
           P    L D APH AIVLKMEDG+ LLLPIIV+E PS++L+AA+RN+ I RPT+Y VVKEM
Sbjct: 131 PRVLRLRDSAPHHAIVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRIPRPTIYNVVKEM 190

Query: 187 IEKMGY----------------------EIGCETDCISFDLRPSDAINIAVRCKVPIQVN 246
            E+MGY                      +IG E + IS DL+PSDAINIA RCKVPIQVN
Sbjct: 191 TERMGYAVRLVRITEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVN 250

Query: 247 KYLAYSDGMKVVETGKLPSQA-PATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLIAAVEE 303
           + +AY++G+KVV+    PS++  ++D   +T +DRP+ QPC EA+EF+L+RNML+AAVEE
Sbjct: 251 RRIAYNNGLKVVQ--PTPSESYVSSDQFQYTRLDRPDDQPCFEAQEFDLVRNMLVAAVEE 310

[Close]

BLAST of Carg08708 vs. Swiss-Prot
Match: sp|B8A8D2|BBD1_ORYSI (Bifunctional nuclease 1 OS=Oryza sativa subsp. indica OX=39946 GN=BBD1 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.1e-71
Identity = 143/259 (55.21%), Postives = 192/259 (74.13%), Query Frame = 0


Query: 67  LCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHLRCVHNN 126
           +CCS+ SSSD +G+ A +++   EE+V SSV+EAVE++S +D F+IKMRDG++LRCV NN
Sbjct: 71  VCCSYGSSSDGDGAAAADYDASGEEFVNSSVMEAVELRSVSDGFVIKMRDGKNLRCVQNN 130

Query: 127 PHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMYQVVKEM 186
           P    L D APH AIVLKMEDG+ LLLPIIV+E PS++L+AA+RN+ I RPT+Y VVKEM
Sbjct: 131 PRVLRLRDSAPHHAIVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRIPRPTIYNVVKEM 190

Query: 187 IEKMGY----------------------EIGCETDCISFDLRPSDAINIAVRCKVPIQVN 246
            E+MGY                      +IG E + IS DL+PSDAINIA RCKVPIQVN
Sbjct: 191 TERMGYAVRLVRITEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVN 250

Query: 247 KYLAYSDGMKVVETGKLPSQA-PATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLIAAVEE 303
           + +AY++G+KVV+    PS++  ++D    T +DRP+ QPC EA+EF+L+RNML+AAVEE
Sbjct: 251 RRIAYNNGLKVVQ--PTPSESYVSSDQFQCTRLDRPDDQPCFEAQEFDLVRNMLVAAVEE 310

[Close]

BLAST of Carg08708 vs. TrEMBL
Match: tr|A0A1S3CRI0|A0A1S3CRI0_CUCME (bifunctional nuclease 1 OS=Cucumis melo OX=3656 GN=LOC103503917 PE=4 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 4.1e-156
Identity = 279/327 (85.32%), Postives = 293/327 (89.60%), Query Frame = 0


Query: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC 60
           MGSLQGPVVCPTIRAKQAGFCSLPINRSLM F FRRGELWGF G NGL AKLP IS +GC
Sbjct: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMAFSFRRGELWGFTGINGLNAKLPAISLKGC 60

Query: 61  ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHL 120
           ARKCKTL CSF+SSSDDNGSTAENFN KDEEYVESSV+EAVEVKSGAD F+IKMRDGRHL
Sbjct: 61  ARKCKTLRCSFSSSSDDNGSTAENFNEKDEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 120

Query: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY 180
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAA+RNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 181 QVVKEMIEKMGYE----------------------IGCETDCISFDLRPSDAINIAVRCK 240
           QVVKEM++KMGYE                      IGCETDC+SFDLRPSDAINIAVRCK
Sbjct: 181 QVVKEMVDKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCETDCLSFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLI 300
           VPIQVNKYLAYSDGM+VVE+GKL +QAPATDGLLFTE+DRP+GQPC EAKEFNL+RNMLI
Sbjct: 241 VPIQVNKYLAYSDGMRVVESGKLSTQAPATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 300

Query: 301 AAVEERYRDAAQWRDKLNLLRARRNLA 306
           AAVEERYRDAAQWRDKLNLLRARRNLA
Sbjct: 301 AAVEERYRDAAQWRDKLNLLRARRNLA 327

[Close]

BLAST of Carg08708 vs. TrEMBL
Match: tr|A0A0A0LDD4|A0A0A0LDD4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G860260 PE=4 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 6.7e-151
Identity = 272/327 (83.18%), Postives = 289/327 (88.38%), Query Frame = 0


Query: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC 60
           MGSLQGPVVCPTI AKQAGFCSLPINRSLM F FRRG+LWGF G NGL AKLP IS + C
Sbjct: 1   MGSLQGPVVCPTICAKQAGFCSLPINRSLMAFSFRRGQLWGFTGINGLNAKLPAISLKRC 60

Query: 61  ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHL 120
            RKCKTL CSF+SSSDDNGSTAENFN K+EEYVESSV+EAVEVKSGAD F+IKMRDGRHL
Sbjct: 61  TRKCKTLRCSFSSSSDDNGSTAENFNEKNEEYVESSVVEAVEVKSGADGFVIKMRDGRHL 120

Query: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY 180
           RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAA+RNVPIARPTMY
Sbjct: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 181 QVVKEMIEKMGYE----------------------IGCETDCISFDLRPSDAINIAVRCK 240
           QVVKEMI+KMGYE                      IGCETDC+SFDLRPSDAINIAVRC+
Sbjct: 181 QVVKEMIDKMGYEVKLVRVTKRVHEAYFAQLYLSKIGCETDCLSFDLRPSDAINIAVRCQ 240

Query: 241 VPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLI 300
           VPIQVNKYLA+SDGM+VVE+GKL +QA ATDGLLFTE+DRP+GQPC EAKEFNL+RNMLI
Sbjct: 241 VPIQVNKYLAFSDGMRVVESGKLSTQALATDGLLFTELDRPSGQPCVEAKEFNLVRNMLI 300

Query: 301 AAVEERYRDAAQWRDKLNLLRARRNLA 306
           AAVEERYRDAAQWRDKLNLLRARRNLA
Sbjct: 301 AAVEERYRDAAQWRDKLNLLRARRNLA 327

[Close]

BLAST of Carg08708 vs. TrEMBL
Match: tr|A0A2N9IGE6|A0A2N9IGE6_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS51410 PE=4 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 2.7e-131
Identity = 235/304 (77.30%), Postives = 265/304 (87.17%), Query Frame = 0


Query: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC 60
           MGSLQGPV+ P++RAKQ GF +LP+    M  R  R E WG  G +  + K+  +S Q  
Sbjct: 1   MGSLQGPVIFPSVRAKQVGFYTLPMIGPFMKARLVRSEFWGLKGISCGKTKVGILSHQLH 60

Query: 61  ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHL 120
            +KCKT+ CS++SSSD NGSTAENFN  DE+YV SSVIEAVEVKSGAD F+IKMRDGRHL
Sbjct: 61  LQKCKTVHCSYSSSSDGNGSTAENFNVNDEDYVNSSVIEAVEVKSGADGFMIKMRDGRHL 120

Query: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY 180
           RCVHNNP GGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAA+RNVPIARPT+Y
Sbjct: 121 RCVHNNPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTLY 180

Query: 181 QVVKEMIEKMGYEIGCETDCISFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMKVVETGK 240
           QVVKEMI+KMGYE+G ET+C+SFDLRPSDAINIAVRCKVPIQVNKYLAYSDGM+V+E+GK
Sbjct: 181 QVVKEMIDKMGYEVGNETECVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRVIESGK 240

Query: 241 LPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLIAAVEERYRDAAQWRDKLNLLRA 300
           L +  PA+DGLLFTE+DRPNGQPC E KEFNL+RNMLIAAVEERYRDAAQWRDKL+ LRA
Sbjct: 241 LLAHTPASDGLLFTEMDRPNGQPCIETKEFNLVRNMLIAAVEERYRDAAQWRDKLSQLRA 300

Query: 301 RRNL 305
           +RNL
Sbjct: 301 KRNL 304

[Close]

BLAST of Carg08708 vs. TrEMBL
Match: tr|A0A2P5F6I8|A0A2P5F6I8_9ROSA (Bifunctional nuclease domain containing protein OS=Trema orientalis OX=63057 GN=TorRG33x02_106730 PE=4 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 4.4e-126
Identity = 236/328 (71.95%), Postives = 264/328 (80.49%), Query Frame = 0


Query: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPG-ISQQG 60
           MGSL GPV+CP+IRAKQAGF +L      +  R  + ELWGF G NG      G +S+Q 
Sbjct: 1   MGSLHGPVICPSIRAKQAGFYTLAPVGFPVKGRILKSELWGFKGINGSSITKAGFLSRQV 60

Query: 61  CARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRH 120
             RKCKT+ C+F+SSS+ NGS AENFN  DE+YV SSV+EAVEVKSG D F+IKMRDGRH
Sbjct: 61  SVRKCKTVNCTFSSSSNGNGSMAENFNENDEDYVNSSVLEAVEVKSGVDGFMIKMRDGRH 120

Query: 121 LRCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTM 180
           LRCVHNNP GGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAA+RNV IARPT+
Sbjct: 121 LRCVHNNPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVQIARPTL 180

Query: 181 YQVVKEMIEKMGYE----------------------IGCETDCISFDLRPSDAINIAVRC 240
           YQVVKEMIEKMGYE                      +G ++DCIS DLRPSDAINIAVRC
Sbjct: 181 YQVVKEMIEKMGYEVKLVRVTKRVHEAYFAQLYLTKVGDDSDCISLDLRPSDAINIAVRC 240

Query: 241 KVPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNML 300
           KVPIQVNKYLAYSDGM+V+E+GKL +Q PA+DGLLFTE+DRP+GQPC E KEFNL+RNML
Sbjct: 241 KVPIQVNKYLAYSDGMRVIESGKLLAQGPASDGLLFTELDRPSGQPCVETKEFNLVRNML 300

Query: 301 IAAVEERYRDAAQWRDKLNLLRARRNLA 306
           IAAVEERYRDAAQWRDKL+ LRARRNLA
Sbjct: 301 IAAVEERYRDAAQWRDKLSQLRARRNLA 328

[Close]

BLAST of Carg08708 vs. TrEMBL
Match: tr|M5XLD1|M5XLD1_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G375100 PE=4 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.9e-125
Identity = 231/327 (70.64%), Postives = 263/327 (80.43%), Query Frame = 0


Query: 1   MGSLQGPVVCPTIRAKQAGFCSLPINRSLMDFRFRRGELWGFAGTNGLRAKLPGISQQGC 60
           M SLQGPV+CPT+RAKQ+G+ ++P+   L+  R  R EL  F G +G   K   +S+   
Sbjct: 1   MVSLQGPVICPTVRAKQSGYDTVPVVGPLVKARLLRSELCRFRGVSGCITKAGFVSRPQN 60

Query: 61  ARKCKTLCCSFNSSSDDNGSTAENFNGKDEEYVESSVIEAVEVKSGADEFLIKMRDGRHL 120
           ARKC  + C+F+SSS+ NGS AENFN  DE+YV SSV+EAVEVKSG D F+IKMRDGRH+
Sbjct: 61  ARKCTRVHCTFSSSSNGNGSMAENFNENDEDYVNSSVLEAVEVKSGIDGFMIKMRDGRHM 120

Query: 121 RCVHNNPHGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVPIARPTMY 180
           RCVHNNP GGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAA+RNVPIARPTMY
Sbjct: 121 RCVHNNPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVPIARPTMY 180

Query: 181 QVVKEMIEKMGYEI----------------------GCETDCISFDLRPSDAINIAVRCK 240
           QVV+EMI+KMGYE+                      G ET+C+SFDLRPSDAINIAVRCK
Sbjct: 181 QVVREMIDKMGYEVRLVRVTKRVHEAYFAQLYLRKAGSETECVSFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNKYLAYSDGMKVVETGKLPSQAPATDGLLFTEVDRPNGQPCAEAKEFNLIRNMLI 300
           VPIQVNKYLAYSDGM+V+E+GKL S  PA+DGLLFTE+DRP+GQPC E KEFNL+RNMLI
Sbjct: 241 VPIQVNKYLAYSDGMRVIESGKLSSHGPASDGLLFTELDRPSGQPCVETKEFNLVRNMLI 300

Query: 301 AAVEERYRDAAQWRDKLNLLRARRNLA 306
           AAVEERYRDAAQWRDKL  LRARRNLA
Sbjct: 301 AAVEERYRDAAQWRDKLLQLRARRNLA 327

The following BLAST results are available for this feature:

BLAST of Carg08708 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022937113.1	7.5e-170	92.97	bifunctional nuclease 1-like [Cucurbita moschata] >XP_022937114.1 bifunctional n...	[more]
XP_023536147.1	1.9e-168	92.05	bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023536148.1 bifunc...	[more]
XP_022975541.1	3.9e-166	91.13	bifunctional nuclease 1-like [Cucurbita maxima] >XP_022975542.1 bifunctional nuc...	[more]
XP_008466529.1	6.2e-156	85.32	PREDICTED: bifunctional nuclease 1 [Cucumis melo] >XP_008466530.1 PREDICTED: bif...	[more]
XP_023525215.1	4.1e-152	82.87	bifunctional nuclease 1-like [Cucurbita pepo subsp. pepo] >XP_023525216.1 bifunc...	[more]

BLAST of Carg08708 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G75380.1	7.4e-111	63.30	bifunctional nuclease in basal defense response 1	[more]
AT1G19660.1	2.8e-110	61.66	Wound-responsive family protein	[more]
AT5G66050.1	7.3e-18	33.33	Wound-responsive family protein	[more]

BLAST of Carg08708 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9FWS6\|BBD1_ARATH	1.3e-109	63.30	Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1	[more]
sp\|Q93VH2\|BBD2_ARATH	5.0e-109	61.66	Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1	[more]
sp\|Q6YZM6\|BBD2_ORYSJ	1.4e-87	55.45	Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV...	[more]
sp\|Q5N8J3\|BBD1_ORYSJ	2.9e-72	55.21	Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV...	[more]
sp\|B8A8D2\|BBD1_ORYSI	1.1e-71	55.21	Bifunctional nuclease 1 OS=Oryza sativa subsp. indica OX=39946 GN=BBD1 PE=3 SV=1	[more]

BLAST of Carg08708 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3CRI0\|A0A1S3CRI0_CUCME	4.1e-156	85.32	bifunctional nuclease 1 OS=Cucumis melo OX=3656 GN=LOC103503917 PE=4 SV=1	[more]
tr\|A0A0A0LDD4\|A0A0A0LDD4_CUCSA	6.7e-151	83.18	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G860260 PE=4 SV=1	[more]
tr\|A0A2N9IGE6\|A0A2N9IGE6_FAGSY	2.7e-131	77.30	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS51410 PE=4 SV=1	[more]
tr\|A0A2P5F6I8\|A0A2P5F6I8_9ROSA	4.4e-126	71.95	Bifunctional nuclease domain containing protein OS=Trema orientalis OX=63057 GN=...	[more]
tr\|M5XLD1\|M5XLD1_PRUPE	2.9e-125	70.64	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G375100 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004518	nuclease activity

Vocabulary: **INTERPRO**
Term	Definition
IPR003729	Bi_nuclease_dom
IPR036104	BFN_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004518	nuclease activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08708-RA	Carg08708-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036104	Bifunctional nuclease superfamily	GENE3D	G3DSA:3.10.690.10		coord: 195..240 e-value: 1.4E-7 score: 32.7
IPR036104	Bifunctional nuclease superfamily	SUPERFAMILY	SSF103256	Hypothetical protein TM0160	coord: 122..238
IPR003729	Bifunctional nuclease domain	PFAM	PF02577	DNase-RNase	coord: 138..194 e-value: 1.1E-4 score: 21.7 coord: 201..232 e-value: 9.0E-6 score: 25.3
None	No IPR available	PANTHER	PTHR15160	VON HIPPEL-LINDAU PROTEIN	coord: 37..194
None	No IPR available	PANTHER	PTHR15160	VON HIPPEL-LINDAU PROTEIN	coord: 195..303
None	No IPR available	PANTHER	PTHR15160:SF3	BIFUNCTIONAL NUCLEASE 1-RELATED	coord: 195..303 coord: 37..194

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg08708	Cla020788	Watermelon (97103) v1	carwmB0102
Carg08708	Csa3G860260	Cucumber (Chinese Long) v2	carcuB0111
Carg08708	MELO3C003799	Melon (DHL92) v3.5.1	carmeB0106
Carg08708	CSPI03G42980	Wild cucumber (PI 183967)	carcpiB0116
Carg08708	Cucsa.342630	Cucumber (Gy14) v1	carcgyB0126
Carg08708	CmaCh01G019290	Cucurbita maxima (Rimu)	carcmaB0136
Carg08708	CmaCh09G001390	Cucurbita maxima (Rimu)	carcmaB0134
Carg08708	CmoCh09G001350	Cucurbita moschata (Rifu)	carcmoB0144
Carg08708	CmoCh01G019920	Cucurbita moschata (Rifu)	carcmoB0146
Carg08708	Lsi04G016960	Bottle gourd (USVL1VR-Ls)	carlsiB094
Carg08708	Cp4.1LG06g00830	Cucurbita pepo (Zucchini)	carcpeB0154
Carg08708	Cp4.1LG02g07570	Cucurbita pepo (Zucchini)	carcpeB0153
Carg08708	CsGy3G040530	Cucumber (Gy14) v2	carcgybB0111
Carg08708	MELO3C003799.2	Melon (DHL92) v3.6.1	carmedB0108
Carg08708	CsaV3_3G045430	Cucumber (Chinese Long) v3	carcucB0120
Carg08708	Cla97C05G100120	Watermelon (97103) v2	carwmbB0107
Carg08708	Bhi09G001865	Wax gourd	carwgoB0121

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg08708	Carg17161	Silver-seed gourd	carcarB125

The following block(s) are covering this gene:


Gene	Organism	Block
Carg08708	Watermelon (97103) v2	carwmbB0105
Carg08708	Watermelon (97103) v2	carwmbB0109
Carg08708	Wax gourd	carwgoB0118
Carg08708	Wax gourd	carwgoB0120
Carg08708	Silver-seed gourd	carcarB119
Carg08708	Silver-seed gourd	carcarB121
Carg08708	Silver-seed gourd	carcarB123
Carg08708	Silver-seed gourd	carcarB126
Carg08708	Cucumber (Gy14) v2	carcgybB0110
Carg08708	Cucumber (Gy14) v2	carcgybB0114
Carg08708	Cucumber (Gy14) v1	carcgyB0124
Carg08708	Cucurbita maxima (Rimu)	carcmaB0135
Carg08708	Cucurbita maxima (Rimu)	carcmaB0137
Carg08708	Cucurbita moschata (Rifu)	carcmoB0145
Carg08708	Cucurbita moschata (Rifu)	carcmoB0147
Carg08708	Cucurbita pepo (Zucchini)	carcpeB0152
Carg08708	Cucurbita pepo (Zucchini)	carcpeB0155
Carg08708	Wild cucumber (PI 183967)	carcpiB0115
Carg08708	Cucumber (Chinese Long) v2	carcuB0110
Carg08708	Cucumber (Chinese Long) v3	carcucB0119
Carg08708	Bottle gourd (USVL1VR-Ls)	carlsiB096
Carg08708	Melon (DHL92) v3.5.1	carmeB0105
Carg08708	Melon (DHL92) v3.6.1	carmedB0107
Carg08708	Watermelon (Charleston Gray)	carwcgB0103
Carg08708	Watermelon (Charleston Gray)	carwcgB0104
Carg08708	Watermelon (Charleston Gray)	carwcgB0106
Carg08708	Watermelon (97103) v1	carwmB0101