Carg08558 (gene) Silver-seed gourd

NameCarg08558
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionUbiquitin protein ligase E3 component n-recognin 7
LocationCucurbita_argyrosperma_scaffold_022 : 1030200 .. 1034059 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTCTCCTGGGCCGTATTCCGCGGGGCTGGCTTTTTGGTACCTTTAAATTGGAAGCGGAAGTTCCATTCGGTAGCCGGTTGCCCGACGGGTCACACGGTCCTCGAGCCTGAGCCAGGTTTGGAGTTTGGGTTTGACATGTCTCAACTGATTTGCATAAAAACCGTGATACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTCTGAAAATATAATGAAATATTATCAGATTTTCATATAAATAAATTATTTTTAACCATTTTATTCTATTATTAAATTTTAGATAATAAAAATTATTAAAATAATTTTAATTAGCTAAAATCTAATTTAATTTTCTAAATTATTAACTTTATTTTCAGTAAATCTTAATAATTATATTCAAATAAATAATTTTAATAAAATTATATAAAATTTTGATCTATTTTATATTTTAAAAATTAAATATTGTTTGGAGAATAAATTATATTGAATGAGATATACCGCTATAATAATTTATTAATATAAAGATAAAAAAGAATTAAAAAAAGATTTATGTATAAGATAATTATTCAATAGAATACTTAATTGATTTTTATTAATTGCAATTTATTATAATTAATGAGGATTTTTCCCTCTACAATTTGAACATGACTTCTGGTTTTGGGTTGAAGAAATTAAAATAAATCCATGAGGCTTAGAACTTGGAAGTGGAGGGAAGAAGATGGAGGGAAGTACATATTCTTTTGCTAATTGTGGCGGGAAAATGTCGTAAGAGACCCCAACCCATTTCCATTTACTATCCCTCTCGCTATCATTCCTCCGATACTCTCTCCTCTCTCTCCTCTTCCTAAGGGGGAAAAAGCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAATCGACTCTTCTTGTGTTGTGCTTCAATTTCAACGCTTTCCGGTGCCGGAGGGGTCTTTCAGCGGCAAATTAGGTACTTTTTTTTCTCTTTAATTTCGTGGGATCGCACCCGTTTGTTCTAGGGCATCGCCATCGCTGTTATCGCGAATTTTCGTTTTGGGGTTTTGAGTGTGACTTCTGTTTTTGGGTTGTTTGATCTTCCGATTTGGGTCCTCAGAACTAGTTTCGATTATCGGAAGTGGAGTGTTGTTGAAGGTTACTGCAGAGTTCGATATCAAATGGCGGAAGTTTTTGATGACGAAACCGAACAGACGATTTCTATTCAAGAGTATCTGAAGGAAGTTGAGGAACAGGAGCTGGTAACTCACTATCTCCCACTCTTCGAGTAAAGGTGTCTGATGTTTGGTTGGTTTTCCTAAAATTTGCGATGTAATGGCCTGGAAACGTACTGGGTTTTCTATAATTAGGGTTCATGGCGTGTTCATATCTTCAGACCTTATTTTATTTCTTATAATCGATTGGGGCGTCCTATTTATTGTTAGTTTATCACCTGTCGCGACATTTTGTTTGTGCTCCAGTGCGAGTAGGAAGATAGTACCTGTAATCTATACTGTTCTAAAGTATGCATTTAACCAAGGTTATTCTGCCTTTCCACAGAATATGATACAACTTTAATGCTTTCTTTTCTTGTTTCTGTATATCCTTTTTGAGTGCTTGGTATCTGATTATTGTTATCATTATGTTATATAATCAAGGAAGCAGATTTGGTTTTGGGAGGTGACGAAGGAAAGGAATGTACTTACAGCAAGGGTTATTTGAAGAGACAAGCCATTTTCTCTTGTCTAACTTGTACCCCTGATGGAAATGCTGGAGTTTGCACTGCGTGTAGCTTGTCCTGTCATGATGGACACGAGGTATGCATGTTATGAAAAGCAACTCTGTTTAGCATATGCAGTGATTTAAGTTCTTTTGTTCAGTGTATAGTGCCAAGCTATAGGCTTGTTGTCTGAGGAAGCATTTAGTGGTTCCTTATGTTGTATATCTATGTAACAGCCTAAGCCCACCGCTAATAGATATTGTTCGCTTTGGCCCGTTACATATCTCCGTTAGTTCACGGTTTTAAAACGTGTCTATTAGGGAGAGGCTTCTACTCCCTTGTAAGGAATGTTTCATTTCCCTCTCCAACCGATGTGGGATCTCACAATCTAGGTCCATTGATTCATGAACATAATCATTTCAAATATTGATCGTTGGTATTCCCAGGTTGCATGAATGATTTATATCAATTGTTTTGACTTCATTTCAATTACGTTAAACTTTCCAAGTACTTTTCTTCCTAACCCCCTCGTGTTATTATGGTCGTCGTTCTATTGGGCAAGATTGTGGAATTATGGACGAAAAGGAACTTCAGGTGCGACTGTGGAAATTCAAAATTTGGAGATTTCATTTGCAAGCTGTTCTCAAACAAAGATGTAGAAAATGCCAACAATTCGTATAACCATAACTTTAGAGGCTCATATTGCATATGCAATCGTCCTTATCCTGATCCAGATGTTGAAGAGCAGGTGGAGATGATCCAGTGCTGTATGTGTGAGGATTGGTTCCATGAGGAACATCTTGGTCTCAGTTCCATAGATGAGGTGTGTTAGCTTAAAATCCTTTCATGGAATTACAATCACCGAGTAAATTCTTATCCTTATAAGAAAATTACTTCGCCTTTTATGTCTATGATGTTAGAATTTTCTTACTGTGGATTTAAGCATCACATGCATTTGTTTCTTCTGCAGATTCCGAGAGACGAAGACGGGGAGCCCCTGTACGAGGACTTCATATGCCCGACATGCTCTGCAGTTTGTTCTTTTCTGAGTCAATACCCTTCTTCTATTTGGGCTGTGGGAAGGCCCTCTGATATCTCCTTGGACGTTACCATGAAAAATGATGCTCCAGAACCCATACTTTCACTTAGTACATCTGTAAAGCACGATGATGATACCAATGCTCACGATTCTGCCAATGTCGACCTTACAGTAGCTCCTGATAAATCTGCTGACAAAAACAGCTTGCTTAGTGAACCTACTACTGAGAATGATCGTAGTTCCAACGAATCTATTAAAGATAACGGTCAAACTGTTAGTTGTGTGCTTGGCGCTTCTCAGTCATCTGCTTCTATAGCCGTTCAAAACAAGCCAATGTTTCTTTCAAAGAACTGGAGGGGTGCCTTGTGCAGATGTGAAAAATGCGTCGATAATTTTAAGCAGATGAATATTAGTTTCCTGCTAGATGAAGAGGACTCCATTGCTGAGTATGAGAAAATAGCAAAGCAGAAGAGGGAAGAGAAGTTACAGCAACAAGAGGGTGCTGAGTTGAAGTTGTTTGATAACCTTGGCCATGTAGAGAAAATTGAGATTCTAAATGGCATTGCAGATATGAAGGATGAGATTCATACATTCCTGGTATTTAATCAGTTTCAATATTTCCCTTGTTTTTGTGTCTATAATGATGAATAAATGTCAATGAATATACATTTTCTTCTTTTCAGGAATCGTTCGACTCGACAAAGCCTATCACATCTGCAGATATCCACCAGGTTTTCGAGAATCTAGCTAAAAAGAGGCGTCGTACAGAATGAAGTTTTCTGAGGAACCTCAGTAGATTTCAGCATCTATGGTCTTAGGATTTCTCAAGTAGGCTGTCCTGACTAAATATACTCTTGGGCTGCTGTCTTTTTTTCAGAATACTGTCGTGTAAAACTGTTAACTATGGGACTTAATTCTCCTTGAGAACTAGATTATGCTTTCTGAAATGACTTGTTTTGGAAACCATGTTAGTAGGCTTACCGGAGGTGCTAAGCTGTAAATTCAACTTAGCTTCAGACCCTATTTCTAATTTATGCTACACCTGGTCCTGATGGATTGTGTTGCTCACTCTGTACATTTGATCCCTTTTCCTCATTAAATGTTCTTTTAGCATCTGC

mRNA sequence

AAATTCTCCTGGGCCGTATTCCGCGGGGCTGGCTTTTTGGTACCTTTAAATTGGAAGCGGAAGTTCCATTCGGTAGCCGGTTGCCCGACGGGTCACACGGTCCTCGAGCCTGAGCCAGGTTACTGCAGAGTTCGATATCAAATGGCGGAAGTTTTTGATGACGAAACCGAACAGACGATTTCTATTCAAGAGTATCTGAAGGAAGTTGAGGAACAGGAGCTGGAAGCAGATTTGGTTTTGGGAGGTGACGAAGGAAAGGAATGTACTTACAGCAAGGGTTATTTGAAGAGACAAGCCATTTTCTCTTGTCTAACTTGTACCCCTGATGGAAATGCTGGAGTTTGCACTGCGTGTAGCTTGTCCTGTCATGATGGACACGAGATTGTGGAATTATGGACGAAAAGGAACTTCAGGTGCGACTGTGGAAATTCAAAATTTGGAGATTTCATTTGCAAGCTGTTCTCAAACAAAGATGTAGAAAATGCCAACAATTCGTATAACCATAACTTTAGAGGCTCATATTGCATATGCAATCGTCCTTATCCTGATCCAGATGTTGAAGAGCAGGTGGAGATGATCCAGTGCTGTATGTGTGAGGATTGGTTCCATGAGGAACATCTTGGTCTCAGTTCCATAGATGAGATTCCGAGAGACGAAGACGGGGAGCCCCTGTACGAGGACTTCATATGCCCGACATGCTCTGCAGTTTGTTCTTTTCTGAGTCAATACCCTTCTTCTATTTGGGCTGTGGGAAGGCCCTCTGATATCTCCTTGGACGTTACCATGAAAAATGATGCTCCAGAACCCATACTTTCACTTAGTACATCTGTAAAGCACGATGATGATACCAATGCTCACGATTCTGCCAATGTCGACCTTACAGTAGCTCCTGATAAATCTGCTGACAAAAACAGCTTGCTTAGTGAACCTACTACTGAGAATGATCGTAGTTCCAACGAATCTATTAAAGATAACGGTCAAACTGTTAGTTGTGTGCTTGGCGCTTCTCAGTCATCTGCTTCTATAGCCGTTCAAAACAAGCCAATGTTTCTTTCAAAGAACTGGAGGGGTGCCTTGTGCAGATGTGAAAAATGCGTCGATAATTTTAAGCAGATGAATATTAGTTTCCTGCTAGATGAAGAGGACTCCATTGCTGAGTATGAGAAAATAGCAAAGCAGAAGAGGGAAGAGAAGTTACAGCAACAAGAGGGTGCTGAGTTGAAGTTGTTTGATAACCTTGGCCATGTAGAGAAAATTGAGATTCTAAATGGCATTGCAGATATGAAGGATGAGATTCATACATTCCTGGAATCGTTCGACTCGACAAAGCCTATCACATCTGCAGATATCCACCAGGTTTTCGAGAATCTAGCTAAAAAGAGGCGTCGTACAGAATGAAGTTTTCTGAGGAACCTCAGTAGATTTCAGCATCTATGGTCTTAGGATTTCTCAAGTAGGCTGTCCTGACTAAATATACTCTTGGGCTGCTGTCTTTTTTTCAGAATACTGTCGTGTAAAACTGTTAACTATGGGACTTAATTCTCCTTGAGAACTAGATTATGCTTTCTGAAATGACTTGTTTTGGAAACCATGTTAGTAGGCTTACCGGAGGTGCTAAGCTGTAAATTCAACTTAGCTTCAGACCCTATTTCTAATTTATGCTACACCTGGTCCTGATGGATTGTGTTGCTCACTCTGTACATTTGATCCCTTTTCCTCATTAAATGTTCTTTTAGCATCTGC

Coding sequence (CDS)

AAATTCTCCTGGGCCGTATTCCGCGGGGCTGGCTTTTTGGTACCTTTAAATTGGAAGCGGAAGTTCCATTCGGTAGCCGGTTGCCCGACGGGTCACACGGTCCTCGAGCCTGAGCCAGGTTACTGCAGAGTTCGATATCAAATGGCGGAAGTTTTTGATGACGAAACCGAACAGACGATTTCTATTCAAGAGTATCTGAAGGAAGTTGAGGAACAGGAGCTGGAAGCAGATTTGGTTTTGGGAGGTGACGAAGGAAAGGAATGTACTTACAGCAAGGGTTATTTGAAGAGACAAGCCATTTTCTCTTGTCTAACTTGTACCCCTGATGGAAATGCTGGAGTTTGCACTGCGTGTAGCTTGTCCTGTCATGATGGACACGAGATTGTGGAATTATGGACGAAAAGGAACTTCAGGTGCGACTGTGGAAATTCAAAATTTGGAGATTTCATTTGCAAGCTGTTCTCAAACAAAGATGTAGAAAATGCCAACAATTCGTATAACCATAACTTTAGAGGCTCATATTGCATATGCAATCGTCCTTATCCTGATCCAGATGTTGAAGAGCAGGTGGAGATGATCCAGTGCTGTATGTGTGAGGATTGGTTCCATGAGGAACATCTTGGTCTCAGTTCCATAGATGAGATTCCGAGAGACGAAGACGGGGAGCCCCTGTACGAGGACTTCATATGCCCGACATGCTCTGCAGTTTGTTCTTTTCTGAGTCAATACCCTTCTTCTATTTGGGCTGTGGGAAGGCCCTCTGATATCTCCTTGGACGTTACCATGAAAAATGATGCTCCAGAACCCATACTTTCACTTAGTACATCTGTAAAGCACGATGATGATACCAATGCTCACGATTCTGCCAATGTCGACCTTACAGTAGCTCCTGATAAATCTGCTGACAAAAACAGCTTGCTTAGTGAACCTACTACTGAGAATGATCGTAGTTCCAACGAATCTATTAAAGATAACGGTCAAACTGTTAGTTGTGTGCTTGGCGCTTCTCAGTCATCTGCTTCTATAGCCGTTCAAAACAAGCCAATGTTTCTTTCAAAGAACTGGAGGGGTGCCTTGTGCAGATGTGAAAAATGCGTCGATAATTTTAAGCAGATGAATATTAGTTTCCTGCTAGATGAAGAGGACTCCATTGCTGAGTATGAGAAAATAGCAAAGCAGAAGAGGGAAGAGAAGTTACAGCAACAAGAGGGTGCTGAGTTGAAGTTGTTTGATAACCTTGGCCATGTAGAGAAAATTGAGATTCTAAATGGCATTGCAGATATGAAGGATGAGATTCATACATTCCTGGAATCGTTCGACTCGACAAAGCCTATCACATCTGCAGATATCCACCAGGTTTTCGAGAATCTAGCTAAAAAGAGGCGTCGTACAGAATGA

Protein sequence

KFSWAVFRGAGFLVPLNWKRKFHSVAGCPTGHTVLEPEPGYCRVRYQMAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCTPDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYNHNFRGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYEDFICPTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAHDSANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSASIAVQNKPMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAELKLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSTKPITSADIHQVFENLAKKRRRTE
BLAST of Carg08558 vs. NCBI nr
Match: XP_022958643.1 (putative E3 ubiquitin-protein ligase UBR7 isoform X2 [Cucurbita moschata])

HSP 1 Score: 838.2 bits (2164), Expect = 1.4e-239
Identity = 411/418 (98.33%), Postives = 416/418 (99.52%), Query Frame = 0

Query: 48  MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 107
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 60

Query: 108 PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 167
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNS FGDFICKLFSNKDVENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSNFGDFICKLFSNKDVENANNSYN 120

Query: 168 HNFRGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 227
           HNF+GSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 180

Query: 228 FICPTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAHD 287
           FIC TCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAHD
Sbjct: 181 FICSTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAHD 240

Query: 288 SANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSASIAVQNK 347
           SANVDL+VAPDKSADKNSLL+EPTTENDRSSNESIKDNGQTVSCVLGASQSSASIAVQNK
Sbjct: 241 SANVDLSVAPDKSADKNSLLTEPTTENDRSSNESIKDNGQTVSCVLGASQSSASIAVQNK 300

Query: 348 PMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 407
           PMFLSKNWRGALCRCEKCVDNFK+MNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL
Sbjct: 301 PMFLSKNWRGALCRCEKCVDNFKRMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 360

Query: 408 KLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSTKPITSADIHQVFENLAKKRRRTE 466
           KLFDNLGHVEKIEILNGIADMKDEIHTFLESFDS+KPITSADIHQVFENLAKKRRRTE
Sbjct: 361 KLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSSKPITSADIHQVFENLAKKRRRTE 418

BLAST of Carg08558 vs. NCBI nr
Match: XP_022958642.1 (putative E3 ubiquitin-protein ligase UBR7 isoform X1 [Cucurbita moschata])

HSP 1 Score: 832.4 bits (2149), Expect = 7.5e-238
Identity = 411/422 (97.39%), Postives = 416/422 (98.58%), Query Frame = 0

Query: 48  MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 107
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 60

Query: 108 PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 167
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNS FGDFICKLFSNKDVENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSNFGDFICKLFSNKDVENANNSYN 120

Query: 168 HNFRGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDE----IPRDEDGEP 227
           HNF+GSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDE    IPRDEDGEP
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEFLLQIPRDEDGEP 180

Query: 228 LYEDFICPTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDT 287
           LYEDFIC TCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDT
Sbjct: 181 LYEDFICSTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDT 240

Query: 288 NAHDSANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSASIA 347
           NAHDSANVDL+VAPDKSADKNSLL+EPTTENDRSSNESIKDNGQTVSCVLGASQSSASIA
Sbjct: 241 NAHDSANVDLSVAPDKSADKNSLLTEPTTENDRSSNESIKDNGQTVSCVLGASQSSASIA 300

Query: 348 VQNKPMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQQE 407
           VQNKPMFLSKNWRGALCRCEKCVDNFK+MNISFLLDEEDSIAEYEKIAKQKREEKLQQQE
Sbjct: 301 VQNKPMFLSKNWRGALCRCEKCVDNFKRMNISFLLDEEDSIAEYEKIAKQKREEKLQQQE 360

Query: 408 GAELKLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSTKPITSADIHQVFENLAKKRRR 466
           GAELKLFDNLGHVEKIEILNGIADMKDEIHTFLESFDS+KPITSADIHQVFENLAKKRRR
Sbjct: 361 GAELKLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSSKPITSADIHQVFENLAKKRRR 420

BLAST of Carg08558 vs. NCBI nr
Match: XP_022996061.1 (putative E3 ubiquitin-protein ligase UBR7 isoform X2 [Cucurbita maxima])

HSP 1 Score: 829.7 bits (2142), Expect = 4.9e-237
Identity = 408/418 (97.61%), Postives = 411/418 (98.33%), Query Frame = 0

Query: 48  MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 107
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 60

Query: 108 PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 167
           P GNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN
Sbjct: 61  PAGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120

Query: 168 HNFRGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 227
           HNF GSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED
Sbjct: 121 HNFNGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 180

Query: 228 FICPTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAHD 287
           FIC  CSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDD NAHD
Sbjct: 181 FICSACSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDANAHD 240

Query: 288 SANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSASIAVQNK 347
           SANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSAS A+QNK
Sbjct: 241 SANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSASRAIQNK 300

Query: 348 PMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 407
           PMFLSKNWRGALCRCEKCVDNFK+MNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL
Sbjct: 301 PMFLSKNWRGALCRCEKCVDNFKRMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 360

Query: 408 KLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSTKPITSADIHQVFENLAKKRRRTE 466
           KLFD LGHVEKIEILNGIADMKDEIHTFLESFDS+KPITSADIHQVFENLAKKRRRTE
Sbjct: 361 KLFDKLGHVEKIEILNGIADMKDEIHTFLESFDSSKPITSADIHQVFENLAKKRRRTE 418

BLAST of Carg08558 vs. NCBI nr
Match: XP_023534431.1 (putative E3 ubiquitin-protein ligase UBR7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 824.7 bits (2129), Expect = 1.6e-235
Identity = 406/418 (97.13%), Postives = 409/418 (97.85%), Query Frame = 0

Query: 48  MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 107
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 60

Query: 108 PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 167
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120

Query: 168 HNFRGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 227
           HNF+GSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 180

Query: 228 FICPTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAHD 287
           FIC  CSAVCSFLSQYPSSIWAVGRPSDISLDVT KNDAPEPILS STSVK DDD NAHD
Sbjct: 181 FICSACSAVCSFLSQYPSSIWAVGRPSDISLDVTKKNDAPEPILSHSTSVKRDDDANAHD 240

Query: 288 SANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSASIAVQNK 347
           SANVDLTVAPDKSADKNSLLSEPTTENDR SNESIKDN QTVSCVLGASQSSAS AVQNK
Sbjct: 241 SANVDLTVAPDKSADKNSLLSEPTTENDRGSNESIKDNDQTVSCVLGASQSSASTAVQNK 300

Query: 348 PMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 407
           PMFLSKNWRGALCRCEKCVDNFK+MNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL
Sbjct: 301 PMFLSKNWRGALCRCEKCVDNFKRMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 360

Query: 408 KLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSTKPITSADIHQVFENLAKKRRRTE 466
           KLFDNLGHVEKIEILNGIADMKDEIHTFLESFDS+KPITSADIHQVFENLAKKRRRTE
Sbjct: 361 KLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSSKPITSADIHQVFENLAKKRRRTE 418

BLAST of Carg08558 vs. NCBI nr
Match: XP_022996060.1 (putative E3 ubiquitin-protein ligase UBR7 isoform X1 [Cucurbita maxima])

HSP 1 Score: 823.9 bits (2127), Expect = 2.7e-235
Identity = 408/422 (96.68%), Postives = 411/422 (97.39%), Query Frame = 0

Query: 48  MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 107
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 60

Query: 108 PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 167
           P GNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN
Sbjct: 61  PAGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120

Query: 168 HNFRGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDE----IPRDEDGEP 227
           HNF GSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDE    IPRDEDGEP
Sbjct: 121 HNFNGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEFLLQIPRDEDGEP 180

Query: 228 LYEDFICPTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDT 287
           LYEDFIC  CSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDD 
Sbjct: 181 LYEDFICSACSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDA 240

Query: 288 NAHDSANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSASIA 347
           NAHDSANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSAS A
Sbjct: 241 NAHDSANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSASRA 300

Query: 348 VQNKPMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQQE 407
           +QNKPMFLSKNWRGALCRCEKCVDNFK+MNISFLLDEEDSIAEYEKIAKQKREEKLQQQE
Sbjct: 301 IQNKPMFLSKNWRGALCRCEKCVDNFKRMNISFLLDEEDSIAEYEKIAKQKREEKLQQQE 360

Query: 408 GAELKLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSTKPITSADIHQVFENLAKKRRR 466
           GAELKLFD LGHVEKIEILNGIADMKDEIHTFLESFDS+KPITSADIHQVFENLAKKRRR
Sbjct: 361 GAELKLFDKLGHVEKIEILNGIADMKDEIHTFLESFDSSKPITSADIHQVFENLAKKRRR 420

BLAST of Carg08558 vs. TAIR10
Match: AT4G23860.1 (PHD finger protein-related)

HSP 1 Score: 401.7 bits (1031), Expect = 6.0e-112
Identity = 208/454 (45.81%), Postives = 266/454 (58.59%), Query Frame = 0

Query: 51  VFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCTPDG 110
           VF+DE E TI+I EY++ ++ +EL ADLVLGGDEG ECT+ KGY+KRQAIFSC+TCTP+G
Sbjct: 5   VFEDEAEGTITINEYIERLDAEELAADLVLGGDEGDECTFPKGYMKRQAIFSCITCTPEG 64

Query: 111 NAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYNHNF 170
           NAG+CTAC LSCHDGHE++ELWTKRNFRCDCGNSKFG   CKL  +KD+EN+ NSYNHNF
Sbjct: 65  NAGICTACCLSCHDGHELLELWTKRNFRCDCGNSKFGTLACKLLPSKDIENSENSYNHNF 124

Query: 171 RGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSID----EIPRDEDGEPLYE 230
           +G YC C+RPYPDP+VEEQVEMIQCC+CEDWFHEEHLGL+  D    +IPRDE+ EP+YE
Sbjct: 125 KGLYCTCDRPYPDPNVEEQVEMIQCCLCEDWFHEEHLGLTPSDSVGSQIPRDEESEPIYE 184

Query: 231 DFICPTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAH 290
           DFIC  CS  CSFL+ YP ++W V +     +D T   +A    + L  +    +     
Sbjct: 185 DFICQNCSPACSFLTLYPENLWVVAK-----VDSTGSANACSETIELDKNHMDSEPGQPE 244

Query: 291 DSANVDLTVAPDKS-----------------------------------ADKNSLLSEPT 350
           +  + + +V    S                                              
Sbjct: 245 NGTDAEKSVVGKCSEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 304

Query: 351 TENDRSSNESIKDNGQTVSCVLGASQSSASIAVQNKPMFLSKNWRGALCRCEKCVDNFKQ 410
                        N     CV+    +S     + KP+FL+KNWR  LCRCEKC++ +KQ
Sbjct: 305 XXXXXXXXXXXXXNVPAAGCVIRTDLNSCP-EFEKKPLFLTKNWRNILCRCEKCLEMYKQ 364

Query: 411 MNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAELKLFDNLGHVEKIEILNGIADMKDE 466
             +S+LLD ED+I EYEK AK+K                         E+L+GI D +D 
Sbjct: 365 RKVSYLLDAEDTIVEYEKKAKEKXXXXXXXXXXXXXXXXXXXXXXXXXELLHGIKDFQDG 424

BLAST of Carg08558 vs. Swiss-Prot
Match: sp|Q8BU04|UBR7_MOUSE (Putative E3 ubiquitin-protein ligase UBR7 OS=Mus musculus OX=10090 GN=Ubr7 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.4e-65
Identity = 158/423 (37.35%), Postives = 233/423 (55.08%), Query Frame = 0

Query: 55  ETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCTPDGN--A 114
           E E  +S+ + L+E EE E EA  VLGG + ++C+YS+G + RQA+++C TCTP+G   A
Sbjct: 13  ELEPVVSLVDVLEEDEELENEACAVLGGSDSEKCSYSQGSVGRQALYACSTCTPEGEEPA 72

Query: 115 GVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYNHNFRG 174
           G+C ACS  CH  H++ EL+TKRNFRCDCGNSKF +  CKLF +K   N+ N YN NF G
Sbjct: 73  GICLACSYECHGSHKLFELYTKRNFRCDCGNSKFKNLECKLFPDKSKVNSCNKYNDNFFG 132

Query: 175 SYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYEDFICPT 234
            YC+C RPYPDP+ E   EMIQC +CEDWFH  HLG      IP  E G+  +++ +C  
Sbjct: 133 LYCVCKRPYPDPEDEVPDEMIQCVVCEDWFHGRHLG-----AIP-PESGD--FQEMVCQA 192

Query: 235 CSAVCSFLSQYPSSIWAVGRPS----DISLDVTMKND----APEPILSLSTSVKHDDDTN 294
           C   CSFL  Y + + AV R S     +  + T   D     PE        +K D   +
Sbjct: 193 CMRRCSFLWAYAAQL-AVTRISAEDDGLLPNATGMGDEDVSKPENGAPQDNGLKEDAPEH 252

Query: 295 AHDSANVDLTVAPDKSADKNSLLSEPTTENDRSS---NESIKDNGQTVSCVLGASQSSAS 354
             DS N        K+  KN   S  ++E+D  +    E+IK   Q+ SC L   Q+   
Sbjct: 253 GRDSVN------EVKAEQKNEPCSSSSSESDLQTVFKKENIKTEPQS-SCRLQELQAKQF 312

Query: 355 IAVQNKPMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQ 414
           +  ++   +   NWR  LC C+ C+  + ++++ FL DE D++  YE   K K ++   +
Sbjct: 313 VK-KDAATYWPLNWRSKLCTCQDCMKMYGELDVLFLTDECDTVLAYEN--KGKNDQATDR 372

Query: 415 QEGAELKLFDNLGHVEKIEILNGIADMKDEIHTFLESF-DSTKPITSADIHQVFENLAKK 464
           ++   +    ++  V+++E++    D+K E+  +L+ F D    +   DI Q FE    K
Sbjct: 373 RDPL-MDTLSSMNRVQQVELICEYNDLKTELKDYLKRFADEGTVVKREDIQQFFEEFQSK 415

BLAST of Carg08558 vs. Swiss-Prot
Match: sp|Q8N806|UBR7_HUMAN (Putative E3 ubiquitin-protein ligase UBR7 OS=Homo sapiens OX=9606 GN=UBR7 PE=1 SV=2)

HSP 1 Score: 241.9 bits (616), Expect = 1.4e-62
Identity = 155/424 (36.56%), Postives = 227/424 (53.54%), Query Frame = 0

Query: 55  ETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCTPDGN--A 114
           E E  +S+ + L+E EE E EA  VLGG + ++C+YS+G +KRQA+++C TCTP+G   A
Sbjct: 13  ELEPVVSLVDVLEEDEELENEACAVLGGSDSEKCSYSQGSVKRQALYACSTCTPEGEEPA 72

Query: 115 GVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYNHNFRG 174
           G+C ACS  CH  H++ EL+TKRNFRCDCGNSKF +  CKL  +K   N+ N YN NF G
Sbjct: 73  GICLACSYECHGSHKLFELYTKRNFRCDCGNSKFKNLECKLLPDKAKVNSGNKYNDNFFG 132

Query: 175 SYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYEDFICPT 234
            YCIC RPYPDP+ E   EMIQC +CEDWFH  HLG      IP  E G+  +++ +C  
Sbjct: 133 LYCICKRPYPDPEDEIPDEMIQCVVCEDWFHGRHLG-----AIP-PESGD--FQEMVCQA 192

Query: 235 CSAVCSFLSQYPSSIWAVGRPS--DISLDVTMKNDAPEPILSLSTSVKHDDDTNAHDSAN 294
           C   CSFL  Y + + AV + S  D  L   +     + ++      +H D T   D   
Sbjct: 193 CMKRCSFLWAYAAQL-AVTKISTEDDGLVRNIDGIGDQEVIKPENG-EHQDSTLKEDVPE 252

Query: 295 VDLTVAPDKSADKNSLLSEP----TTENDRSS---NESIKDNGQTVSCVLGASQSSASIA 354
                  +   ++N   SEP    ++E+D  +   NES+  N ++ S        +  + 
Sbjct: 253 QGKDDVREVKVEQN---SEPCAGSSSESDLQTVFKNESL--NAESKSGCKLQELKAKQLI 312

Query: 355 VQNKPMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQQE 414
            ++   +   NWR  LC C+ C+  +  +++ FL DE D++  YE       + K+ Q  
Sbjct: 313 KKDTATYWPLNWRSKLCTCQDCMKMYGDLDVLFLTDEYDTVLAYE------NKGKIAQAT 372

Query: 415 GAELKLFDNL---GHVEKIEILNGIADMKDEIHTFLESF-DSTKPITSADIHQVFENLAK 464
                L D L     V+++E++    D+K E+  +L+ F D    +   DI Q FE    
Sbjct: 373 DRSDPLMDTLSSMNRVQQVELICEYNDLKTELKDYLKRFADEGTVVKREDIQQFFEEFQS 415

BLAST of Carg08558 vs. Swiss-Prot
Match: sp|Q09329|MLO2_SCHPO (Protein mlo2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mlo2 PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 6.4e-31
Identity = 73/183 (39.89%), Postives = 108/183 (59.02%), Query Frame = 0

Query: 54  DETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCTPDGNA- 113
           +ET   +++++Y+++  E E EA  VL       CTYS GYLK Q +++CLTC     + 
Sbjct: 2   EETAHELTVKQYVEQQRELEREAREVL-PYSFDTCTYSMGYLK-QPLYACLTCQKASGSL 61

Query: 114 -GVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYNHNFR 173
             VC +CS+SCH  H++V+L+ KR+FRCDCG ++     C L  + D   + N YNHNF 
Sbjct: 62  NAVCYSCSISCHADHDLVDLFNKRHFRCDCGTTRTHSIPCNLRKSVDECGSENDYNHNFE 121

Query: 174 GSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYEDFICP 233
           G +CIC+  Y +P+ EE   M QC +CEDWFHE+ L  ++      D +    +E  +C 
Sbjct: 122 GRFCICDTVY-NPETEEGT-MFQCILCEDWFHEKCLQKTNKGIAIPDAE---TFEWLVCS 177

Query: 234 TCS 235
            CS
Sbjct: 182 ECS 177

BLAST of Carg08558 vs. Swiss-Prot
Match: sp|Q54DV0|UBR7_DICDI (Putative E3 ubiquitin-protein ligase ubr7 OS=Dictyostelium discoideum OX=44689 GN=ubr7 PE=3 SV=2)

HSP 1 Score: 110.5 bits (275), Expect = 4.9e-23
Identity = 108/432 (25.00%), Postives = 160/432 (37.04%), Query Frame = 0

Query: 82  GDEGKECTYSKGYLKRQAIFSCLTC--TPDGNAGVCTACSLSCHDGHEIVELWTKRNFRC 141
           GDE   CT+ KGY+  Q++F+C TC  + D   G C  CS+ CH  H++ EL+ KRNFRC
Sbjct: 97  GDE-SICTFDKGYI-NQSVFACKTCQLSNDKLFGFCYGCSMHCHLYHDVYELFNKRNFRC 156

Query: 142 DCG---NSKFGDFICKL------------------------------------------- 201
           DCG         F C+L                                           
Sbjct: 157 DCGTKIQEPNNSFKCQLSGILKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLNDI 216

Query: 202 ----FSNKDVENANNSYNHNFRGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGL 261
               +    + N  N YNHNF+G YC C+ PY   D +E  +MIQC  CEDWFHE  L L
Sbjct: 217 DIGSYDKSQILNERNHYNHNFKGKYCYCDSPY---DYKE--DMIQCIFCEDWFHENCLKL 276

Query: 262 -SSIDEIPRDEDGEPLYEDFICPTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPE 321
            S++ +IP    GE  + D IC  C +   FL  YP             +   ++ND   
Sbjct: 277 NSNVTDIP--SPGE--FSDLICADCLSKNQFLLLYP------------QIRCYIEND--H 336

Query: 322 PILSLSTSVKHDDDTNAHDSANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQT 381
            I+                       + P+K  D                          
Sbjct: 337 IIIGXXXXXXXXXXXXXXICKVEGGVITPNKKYD-------------------------- 396

Query: 382 VSCVLGASQSSASIAVQNKPMFLSKNWRGALCRCEKCVDNFKQMNISFLLD-EEDSIAEY 441
                               +F  + W+  LC C K         + FL + +E+S+ + 
Sbjct: 397 --------------------LFCKELWKDELCSCLKXXXXXXXXKVEFLFEKDENSLKKK 456

Query: 442 EKIAKQKREEK-LQQQEGAELKLFDNLGHVEKIEILNGIADMKDEI-HTFLESFDSTKPI 458
            K   +  + K +   E  +      L   ++  ++ G +DMK+++   F +  D  + I
Sbjct: 457 NKTVDENLDNKPVNVFEMGQDVFSKTLPPTQQRALIEGFSDMKEKLKELFSKKLDKNQVI 457

BLAST of Carg08558 vs. TrEMBL
Match: tr|A0A0A0KQR4|A0A0A0KQR4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G149850 PE=4 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 1.5e-205
Identity = 363/418 (86.84%), Postives = 382/418 (91.39%), Query Frame = 0

Query: 48  MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 107
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTY+KGY+KRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYNKGYMKRQAIFSCLTCT 60

Query: 108 PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 167
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFG+F+CKLFSNKDVEN+NNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGNFVCKLFSNKDVENSNNSYN 120

Query: 168 HNFRGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 227
           HNF+GSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDE+GEPLYED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180

Query: 228 FICPTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAHD 287
           FIC  CSAVCSFLSQYPSSIWAVGR SD S+DV+ K DA E  L  STSVKH+DD N HD
Sbjct: 181 FICKACSAVCSFLSQYPSSIWAVGRQSDTSMDVSNKIDASE--LPASTSVKHNDDVNLHD 240

Query: 288 SANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSASIAVQNK 347
           SAN D TV  DKSA K+SLLSE     + SS+E IKDN QT SCVLG S  SASI V+NK
Sbjct: 241 SANADPTVDTDKSAGKDSLLSE--LPENPSSSEPIKDNNQTDSCVLGTS-LSASIIVENK 300

Query: 348 PMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 407
           PMFLSKNWRGALCRCEKC++N+K  NISFLLDEEDSIAEYEK+AKQKREEKL QQEGAE 
Sbjct: 301 PMFLSKNWRGALCRCEKCINNYKLNNISFLLDEEDSIAEYEKMAKQKREEKLLQQEGAES 360

Query: 408 KLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSTKPITSADIHQVFENLAKKRRRTE 466
           KLFDNLGHVEKIEILNGIADMKDEI TFLESFDS+KPITSADIHQVFENLAKKRRRTE
Sbjct: 361 KLFDNLGHVEKIEILNGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 413

BLAST of Carg08558 vs. TrEMBL
Match: tr|A0A1S3AUI5|A0A1S3AUI5_CUCME (putative E3 ubiquitin-protein ligase UBR7 OS=Cucumis melo OX=3656 GN=LOC103482825 PE=4 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 1.9e-197
Identity = 352/418 (84.21%), Postives = 373/418 (89.23%), Query Frame = 0

Query: 48  MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 107
           MAEV DDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTY+KGY+KRQAIFSCLTCT
Sbjct: 1   MAEVSDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYNKGYMKRQAIFSCLTCT 60

Query: 108 PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 167
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFG+F+CKLFSNKDVEN++NSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGNFVCKLFSNKDVENSDNSYN 120

Query: 168 HNFRGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 227
           HNF+GSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDE+GEPLYED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180

Query: 228 FICPTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAHD 287
           FIC  CSAVCSFLS YPSSIWAVGR SD SLDV+ K DA E  L  STS+KH+DD N   
Sbjct: 181 FICKACSAVCSFLSLYPSSIWAVGRQSDTSLDVSNKIDASE--LPASTSLKHNDDVNXXX 240

Query: 288 SANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSASIAVQNK 347
                 TV  DKSA K+SLLSE     + SS++ IKDN QT SCVLG S  SASI V+NK
Sbjct: 241 XXXXXXTVDTDKSAGKDSLLSE--LPENPSSSKPIKDNSQTDSCVLGTS-LSASIIVENK 300

Query: 348 PMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAEL 407
           PMFLSKNWRGALCRCEKC++ +K  NISFLLDEEDSIAEYEK+AKQKREEKL QQEGAE 
Sbjct: 301 PMFLSKNWRGALCRCEKCINTYKLNNISFLLDEEDSIAEYEKMAKQKREEKLLQQEGAES 360

Query: 408 KLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSTKPITSADIHQVFENLAKKRRRTE 466
           KLFDNLGHVEKIEILNGIADMKDEI TFLESFDS+KPITSADIHQVFENLAKKRRRTE
Sbjct: 361 KLFDNLGHVEKIEILNGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 413

BLAST of Carg08558 vs. TrEMBL
Match: tr|A0A2I4E849|A0A2I4E849_9ROSI (putative E3 ubiquitin-protein ligase UBR7 OS=Juglans regia OX=51240 GN=LOC108987156 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 2.3e-166
Identity = 287/419 (68.50%), Postives = 338/419 (80.67%), Query Frame = 0

Query: 48  MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 107
           M++ F+DE EQT+SIQEYLK VEEQELEADLVLGGDEGKECTY  GY+KRQAIFSCLTCT
Sbjct: 1   MSDAFEDEAEQTVSIQEYLKGVEEQELEADLVLGGDEGKECTYINGYMKRQAIFSCLTCT 60

Query: 108 PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 167
           PDG+AGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFG+F CKLF NKD+ENA NSYN
Sbjct: 61  PDGHAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGEFFCKLFPNKDIENAENSYN 120

Query: 168 HNFRGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 227
           HNFRGSYC C+RPYPDPDVEEQVEMIQCC+CEDWFHEEHLGL S DE+PRDE+GEPLYED
Sbjct: 121 HNFRGSYCTCSRPYPDPDVEEQVEMIQCCICEDWFHEEHLGLESSDEVPRDEEGEPLYED 180

Query: 228 FICPTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAHD 287
           FIC  CS   SFL  YP SIWA GR  D ++D     +  E   S   S K  +D +A +
Sbjct: 181 FICRACSTSFSFLGSYPQSIWAAGRQCDATVDTNKDKNVLEDTSSAGGSGKLKNDVDARN 240

Query: 288 SANVDLTVAPDKSA-DKNSLLSEPTTENDRSSNESIKDNGQTVSCVLGASQSSASIAVQN 347
           +  VD T +  + A D + +     ++N+  S++ +K +    +CVLG +  +AS A++N
Sbjct: 241 NPEVDHTKSDSEFACDGDGMSLGENSQNNVGSDQCVKTSSPLTTCVLGVNPENASPALEN 300

Query: 348 KPMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAE 407
           KP+FLSK+WR  LCRCEKC+  + Q  I+FLLD+EDSIAEYEK+A  KR EKLQ++EGAE
Sbjct: 301 KPLFLSKSWRDVLCRCEKCLVIYTQKRIAFLLDKEDSIAEYEKMATLKRTEKLQEREGAE 360

Query: 408 LKLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSTKPITSADIHQVFENLAKKRRRTE 466
           L LF+NLGH+EK+EILNGIADMK+EIH FLESFDSTKPIT+AD+HQVFENLAKKRRR +
Sbjct: 361 LSLFNNLGHIEKMEILNGIADMKNEIHGFLESFDSTKPITAADVHQVFENLAKKRRRLD 419

BLAST of Carg08558 vs. TrEMBL
Match: tr|A0A2N9I6Z5|A0A2N9I6Z5_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS47766 PE=4 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 3.3e-165
Identity = 287/417 (68.82%), Postives = 336/417 (80.58%), Query Frame = 0

Query: 48  MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTCT 107
           MA   D+E EQT+SIQEYLK VEEQELEADLVLGGDEGKECTY+ GY+KRQAIFSCLTCT
Sbjct: 1   MAGELDEEAEQTVSIQEYLKGVEEQELEADLVLGGDEGKECTYTNGYMKRQAIFSCLTCT 60

Query: 108 PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 167
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFG+F CKLF NKD+ENA+NSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGEFFCKLFPNKDIENADNSYN 120

Query: 168 HNFRGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYED 227
           HNF+GSYC C RPYPDPD EEQVEMIQCC+CEDWFHEEHLGL S  E+PRDE+GEPLYED
Sbjct: 121 HNFKGSYCTCGRPYPDPDAEEQVEMIQCCICEDWFHEEHLGLESSGEVPRDEEGEPLYED 180

Query: 228 FICPTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAHD 287
           FIC  CSA  SFL  YP SIWA G+  D ++D     +  E   S   S K D DT+AH+
Sbjct: 181 FICRACSASFSFLGLYPQSIWAAGKQCDATVDTNKDKNVVEDTSSAGGSGKLDTDTDAHN 240

Query: 288 SANVDLTVAPDKSADKNSLLSEPTTENDRSSNESIKD-NGQTVSCVLGASQSSASIAVQN 347
           +   D        +D ++L    +++ +  SN  +KD +  T +CVLG +   AS A+++
Sbjct: 241 NTKTDQA----NVSDGDNLSLGESSQKNVVSNPCVKDASPHTSTCVLGVNLGDASPALES 300

Query: 348 KPMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAE 407
           KP+FLSKNWR  LCRCEKC+  + Q  I+FL+D+EDSIAEYE++A QKR+EKLQQQEGAE
Sbjct: 301 KPLFLSKNWRDVLCRCEKCIVLYNQKRIAFLVDKEDSIAEYEQMATQKRKEKLQQQEGAE 360

Query: 408 LKLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSTKPITSADIHQVFENLAKKRRR 464
           L LF+NLGH+EK+EIL+GIADMKDEI TFLESFDS+KPIT+AD+HQVF+NLAKKRRR
Sbjct: 361 LSLFNNLGHIEKMEILSGIADMKDEIRTFLESFDSSKPITAADVHQVFDNLAKKRRR 413

BLAST of Carg08558 vs. TrEMBL
Match: tr|A0A2P4JUE3|A0A2P4JUE3_QUESU (Putative e3 ubiquitin-protein ligase ubr7 OS=Quercus suber OX=58331 GN=CFP56_67234 PE=4 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 7.6e-162
Identity = 284/422 (67.30%), Postives = 331/422 (78.44%), Query Frame = 0

Query: 47  QMAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYLKRQAIFSCLTC 106
           +MA   +DE EQT+SIQEYLK VEEQELEADLVLGGDEGKECTY+ GY+KRQAIFSCLTC
Sbjct: 94  KMAGELEDEAEQTVSIQEYLKGVEEQELEADLVLGGDEGKECTYTNGYMKRQAIFSCLTC 153

Query: 107 TPDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSY 166
           TPDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFG+F CK+F NKD+ENA NSY
Sbjct: 154 TPDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGEFFCKIFPNKDIENAENSY 213

Query: 167 NHNFRGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEDGEPLYE 226
           NHNF+GSYC C+RPYPDPDVEEQVEMIQCC+CEDWFHEEHLGL    E+PRD++GEPLYE
Sbjct: 214 NHNFKGSYCTCSRPYPDPDVEEQVEMIQCCICEDWFHEEHLGLEPSGEVPRDDEGEPLYE 273

Query: 227 DFICPTCSAVCSFLSQYPSSIWAVGRPSDISLDVTMKNDAPEPILSLSTSVKHDDDTNAH 286
           DFIC  CSA  SFL  YP SIWA GR  D ++D     +  E   S   S K D+D NAH
Sbjct: 274 DFICKACSASFSFLGLYPQSIWAAGRQGDATVDTNKDKNVVEDTSSAGGSGKLDNDVNAH 333

Query: 287 DSANVDL--TVAPDKSADKNSLLSEPTTENDRSSNESIKD-NGQTVSCVLGASQSSASIA 346
           ++  +D               L    +++    S+  +KD +  T +CVLG +    S A
Sbjct: 334 NNPKMDQANVSXXXXXXXXXXLFVAESSQKTVVSDPCVKDASSHTSTCVLGVNLGVDSPA 393

Query: 347 VQNKPMFLSKNWRGALCRCEKCVDNFKQMNISFLLDEEDSIAEYEKIAKQKREEKLQQQE 406
           + +KP+FLSKNWR  LCRCEKC+  + Q +I+FLLD+EDSIAEYE++A QKR+EKLQQQE
Sbjct: 394 LDSKPLFLSKNWRDVLCRCEKCIVLYNQKHIAFLLDKEDSIAEYEQMATQKRKEKLQQQE 453

Query: 407 GAELKLFDNLGHVEKIEILNGIADMKDEIHTFLESFDSTKPITSADIHQVFENLAKKRRR 466
           GAEL LF+ LGHVEK+EIL+GIADMKDEI TFLESFDS+KPIT+ D+HQVF+NLAKKRRR
Sbjct: 454 GAELSLFNKLGHVEKMEILSGIADMKDEIRTFLESFDSSKPITADDVHQVFDNLAKKRRR 513

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022958643.11.4e-23998.33putative E3 ubiquitin-protein ligase UBR7 isoform X2 [Cucurbita moschata][more]
XP_022958642.17.5e-23897.39putative E3 ubiquitin-protein ligase UBR7 isoform X1 [Cucurbita moschata][more]
XP_022996061.14.9e-23797.61putative E3 ubiquitin-protein ligase UBR7 isoform X2 [Cucurbita maxima][more]
XP_023534431.11.6e-23597.13putative E3 ubiquitin-protein ligase UBR7 [Cucurbita pepo subsp. pepo][more]
XP_022996060.12.7e-23596.68putative E3 ubiquitin-protein ligase UBR7 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G23860.16.0e-11245.81PHD finger protein-related[more]
Match NameE-valueIdentityDescription
sp|Q8BU04|UBR7_MOUSE1.4e-6537.35Putative E3 ubiquitin-protein ligase UBR7 OS=Mus musculus OX=10090 GN=Ubr7 PE=1 ... [more]
sp|Q8N806|UBR7_HUMAN1.4e-6236.56Putative E3 ubiquitin-protein ligase UBR7 OS=Homo sapiens OX=9606 GN=UBR7 PE=1 S... [more]
sp|Q09329|MLO2_SCHPO6.4e-3139.89Protein mlo2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN... [more]
sp|Q54DV0|UBR7_DICDI4.9e-2325.00Putative E3 ubiquitin-protein ligase ubr7 OS=Dictyostelium discoideum OX=44689 G... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0KQR4|A0A0A0KQR4_CUCSA1.5e-20586.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G149850 PE=4 SV=1[more]
tr|A0A1S3AUI5|A0A1S3AUI5_CUCME1.9e-19784.21putative E3 ubiquitin-protein ligase UBR7 OS=Cucumis melo OX=3656 GN=LOC10348282... [more]
tr|A0A2I4E849|A0A2I4E849_9ROSI2.3e-16668.50putative E3 ubiquitin-protein ligase UBR7 OS=Juglans regia OX=51240 GN=LOC108987... [more]
tr|A0A2N9I6Z5|A0A2N9I6Z5_FAGSY3.3e-16568.82Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS47766 PE=4 SV=1[more]
tr|A0A2P4JUE3|A0A2P4JUE3_QUESU7.6e-16267.30Putative e3 ubiquitin-protein ligase ubr7 OS=Quercus suber OX=58331 GN=CFP56_672... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
Vocabulary: INTERPRO
TermDefinition
IPR011011Znf_FYVE_PHD
IPR013083Znf_RING/FYVE/PHD
IPR003126Znf_UBR
IPR001965Znf_PHD
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0008270 zinc ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08558-RACarg08558-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 377..397
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 275..324
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 304..324
NoneNo IPR availablePANTHERPTHR13513E3 UBIQUITIN-PROTEIN LIGASE UBR7coord: 48..463
NoneNo IPR availableCDDcd15542PHD_UBR7coord: 174..233
e-value: 4.22151E-23
score: 91.275
IPR001965Zinc finger, PHD-typeSMARTSM00249PHD_3coord: 174..234
e-value: 7.8E-6
score: 35.4
IPR003126Zinc finger, UBR-typeSMARTSM00396push_1coord: 86..155
e-value: 6.9E-21
score: 85.4
IPR003126Zinc finger, UBR-typePFAMPF02207zf-UBRcoord: 88..152
e-value: 5.4E-14
score: 52.0
IPR003126Zinc finger, UBR-typePROSITEPS51157ZF_UBRcoord: 86..156
score: 9.122
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 170..237
e-value: 2.0E-11
score: 45.2
IPR011011Zinc finger, FYVE/PHD-typeSUPERFAMILYSSF57903FYVE/PHD zinc fingercoord: 162..247