Carg08216 (gene) Silver-seed gourd

NameCarg08216
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionSerine hydrolase FSH
LocationCucurbita_argyrosperma_scaffold_015 : 158764 .. 160775 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTTGCTCTCCCCTCTCCTCTGCTCACCAATTTCGAAATCCAGATCTCCGGAGTTTCATTTACGGAGAATTCCAATCATGGGAACCGACGGTGAAATCCGAAGAAAACCTAGATTTCTTTGCCTCCATGGCTTCCGAACCAGTGGAGCAATCCTTAAGAAGCAGGTCGGAAAATGGCCGGATTCTGTCCTTGAACAACTCGATCTGGATTTTCTCGATGCTCCATTTCCTGCCGAAGGCAAATCTGACATCGAAGGAATTTTTGATCCTCCCTATTTTGAGTGGTTTCAATTCAGTCCAGTACGACTGCTTAACCCTAATTTCTTCCGAAAATCGCAAACTCTCGTTTATTTTGTTTGTTTATTCTCTTTTCTTCCATTGCAGGAATTTACAGAGTATAGGAATTTCGATGAGTGTCTCTCATTCATTGAGGATTACATGCTTAAGCACGGACCATTCGATGGATTCCTCGGTTTCTCACAGGTAATTTCCTTTTAATTTTTCCTAATTAGCCAAACGGAGCTTAATCGAGTATCCTGTTGATGAACTTCGTTGAATTTGACAATTTCAATCTCTTCGCGAACAATTTCAGGGGGCGATACTATCGGCCGCTCTGCCGGGACTTCAAGCGAAGGTAACCTAACAGCAGCCTGCTGCTATTACGTTACAAATCCATCTGAATTCTCCAAAATAAACGTTAAAAGTGAAATCGAGGTTTTGAGGCAGAGGTGCATTAATTGTTGCAGGGTATTGCACTGACGAAAGTCCCCAAGATCAAATTCGTGATCATCGTCAGCGGTGCGAAATTCAGATCGCAATCTGTGGCTGAGAAAGCTTACTCGCCAGTCATTGGATGCCCATCCCTCCATTTTCTAGGTACCTCCTCCACATGGCGTTTGTCCTTTTTTGAAAGTAGTTTCCAATATAATGATTCTTTACATCGTTGCTTTGAAAACAAACAAGCCACCGAATGCATAATTTTAGGCATTTAGTTTTGGAATTAAATAACAGCTTGTCTTAATTTCTTGAATTACGTCCTAGGAGTCGAACAATCCATCATATTTTATGCATCAAAATAATTATAGACAGTTGACAATTCAAATTAAGGAGGTTGTTATGAATGTATTATTAAAAAATGTGAACGGACAATACTTTGATTGGTCTTTTTTTTCAAAAATAAGAGAATCAAATCCCTAAATTCTTAGGTGGGTATCACTTTAAAACTCCAAATAGCGCAAAACTTCACTAATTGAATCAATTTAGACATCCAATCCATTATAGACTTTCATTTTAAAGCCATTGATGCAAGAGTTCTTAAGATCCCTTGATTTGATATTTTCCTTTGATTATATGAACTGTTTAGATTCTTGAATTTAAAATTTGAAATAAATTAATCAACTTCTAGAACCTGTGTGTGAAATGCTGAAAAGGGTTATATCATGGTGGTAGGTAAGGAAGATTTCTTGATGCCCCACGGACAGAAGCTTCTGGAATCATATATAGATCCAGTAATAATTAATCATCCTAAAGGCCACACAATACCAAGACTGGGTAATATACAATTTCAACTCATTTCACTTTTCCATCCTTAATTTCTGATGCTGATTTAATTATCTCAACTTTATATTTTCAGATGATGAAGGTTTGGAGGTTATGGAGAGTTTCATTGAGAAGATTTCAAAGATGTTGGATGAGGCAGAAGAATAATAATAGTCTTAAAAGTCGCATTGGACTATCAATTATGCAGCCTTAGCCTTAAACAATGATGGAGCTAATTGTATAGTTCATTCGATGTATTTTATTTTAAATATTTCAAAACTATTCTCTCCGTAGGGTTGAGCCCTTGTATTCCATCAAGTGTTACACATCATTAATCTTACACACAACAAGTAATTTGTCATAGTTTCTGGATCTGTCTTGTGTATAATTAGTAATCTTTTATGGTTCCGAGGGTTTATGGTAAATTTGCCCCAAGGATAAGACAAATACATAATGAGAATTTTCTTACC

mRNA sequence

ATGAGTTTGCTCTCCCCTCTCCTCTGCTCACCAATTTCGAAATCCAGATCTCCGGAGTTTCATTTACGGAGAATTCCAATCATGGGAACCGACGGTGAAATCCGAAGAAAACCTAGATTTCTTTGCCTCCATGGCTTCCGAACCAGTGGAGCAATCCTTAAGAAGCAGGTCGGAAAATGGCCGGATTCTGTCCTTGAACAACTCGATCTGGATTTTCTCGATGCTCCATTTCCTGCCGAAGGCAAATCTGACATCGAAGGAATTTTTGATCCTCCCTATTTTGAGTGGTTTCAATTCAGTCCAGAATTTACAGAGTATAGGAATTTCGATGAGTGTCTCTCATTCATTGAGGATTACATGCTTAAGCACGGACCATTCGATGGATTCCTCGGTTTCTCACAGGGGGCGATACTATCGGCCGCTCTGCCGGGACTTCAAGCGAAGGGTATTGCACTGACGAAAGTCCCCAAGATCAAATTCGTGATCATCGTCAGCGGTGCGAAATTCAGATCGCAATCTGTGGCTGAGAAAGCTTACTCGCCAGTCATTGGATGCCCATCCCTCCATTTTCTAGATGATGAAGGTTTGGAGGTTATGGAGAGTTTCATTGAGAAGATTTCAAAGATGTTGGATGAGGCAGAAGAATAATAATAGTCTTAAAAGTCGCATTGGACTATCAATTATGCAGCCTTAGCCTTAAACAATGATGGAGCTAATTGTATAGTTCATTCGATGTATTTTATTTTAAATATTTCAAAACTATTCTCTCCGTAGGGTTGAGCCCTTGTATTCCATCAAGTGTTACACATCATTAATCTTACACACAACAAGTAATTTGTCATAGTTTCTGGATCTGTCTTGTGTATAATTAGTAATCTTTTATGGTTCCGAGGGTTTATGGTAAATTTGCCCCAAGGATAAGACAAATACATAATGAGAATTTTCTTACC

Coding sequence (CDS)

ATGAGTTTGCTCTCCCCTCTCCTCTGCTCACCAATTTCGAAATCCAGATCTCCGGAGTTTCATTTACGGAGAATTCCAATCATGGGAACCGACGGTGAAATCCGAAGAAAACCTAGATTTCTTTGCCTCCATGGCTTCCGAACCAGTGGAGCAATCCTTAAGAAGCAGGTCGGAAAATGGCCGGATTCTGTCCTTGAACAACTCGATCTGGATTTTCTCGATGCTCCATTTCCTGCCGAAGGCAAATCTGACATCGAAGGAATTTTTGATCCTCCCTATTTTGAGTGGTTTCAATTCAGTCCAGAATTTACAGAGTATAGGAATTTCGATGAGTGTCTCTCATTCATTGAGGATTACATGCTTAAGCACGGACCATTCGATGGATTCCTCGGTTTCTCACAGGGGGCGATACTATCGGCCGCTCTGCCGGGACTTCAAGCGAAGGGTATTGCACTGACGAAAGTCCCCAAGATCAAATTCGTGATCATCGTCAGCGGTGCGAAATTCAGATCGCAATCTGTGGCTGAGAAAGCTTACTCGCCAGTCATTGGATGCCCATCCCTCCATTTTCTAGATGATGAAGGTTTGGAGGTTATGGAGAGTTTCATTGAGAAGATTTCAAAGATGTTGGATGAGGCAGAAGAATAA

Protein sequence

MSLLSPLLCSPISKSRSPEFHLRRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHFLDDEGLEVMESFIEKISKMLDEAEE
BLAST of Carg08216 vs. NCBI nr
Match: XP_022961839.1 (dihydrofolate reductase-like [Cucurbita moschata])

HSP 1 Score: 416.0 bits (1068), Expect = 7.8e-113
Identity = 213/249 (85.54%), Postives = 214/249 (85.94%), Query Frame = 0

Query: 1   MSLLSPLLCSPISKSRSPEFHLRRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKW 60
           MS LSPLLCSPISKSRSPEFHL+RIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKW
Sbjct: 1   MSSLSPLLCSPISKSRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKW 60

Query: 61  PDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYM 120
           PDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYM
Sbjct: 61  PDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYM 120

Query: 121 LKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYS 180
           LKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYS
Sbjct: 121 LKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYS 180

Query: 181 PVIGCPSLHF----------------------------------LDDEGLEVMESFIEKI 216
           PVIGCPSLHF                                  LDDEGLEVMESFIEKI
Sbjct: 181 PVIGCPSLHFLGKEDFLMPHGQKLLESYIEPVIINHPKGHTIPRLDDEGLEVMESFIEKI 240

BLAST of Carg08216 vs. NCBI nr
Match: XP_023545459.1 (dihydrofolate reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 407.5 bits (1046), Expect = 2.8e-110
Identity = 209/249 (83.94%), Postives = 211/249 (84.74%), Query Frame = 0

Query: 1   MSLLSPLLCSPISKSRSPEFHLRRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKW 60
           MS LSPLLCSPISK RSPEFHL+RIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKW
Sbjct: 1   MSSLSPLLCSPISKYRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKW 60

Query: 61  PDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYM 120
           PDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYM
Sbjct: 61  PDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYM 120

Query: 121 LKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYS 180
           LKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYS
Sbjct: 121 LKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYS 180

Query: 181 PVIGCPSLHF----------------------------------LDDEGLEVMESFIEKI 216
             IGCPSLHF                                  LDD+GLEVMESFIEKI
Sbjct: 181 SAIGCPSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESFIEKI 240

BLAST of Carg08216 vs. NCBI nr
Match: XP_022997250.1 (dihydrofolate reductase-like [Cucurbita maxima])

HSP 1 Score: 407.1 bits (1045), Expect = 3.6e-110
Identity = 208/249 (83.53%), Postives = 211/249 (84.74%), Query Frame = 0

Query: 1   MSLLSPLLCSPISKSRSPEFHLRRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKW 60
           MS LSPLLCSPISK+RSPEFHL+RIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKW
Sbjct: 1   MSSLSPLLCSPISKTRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKW 60

Query: 61  PDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYM 120
           PDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYM
Sbjct: 61  PDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYM 120

Query: 121 LKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYS 180
           LKHGPFDGFLGFSQGAILSAALPGLQAKG+ALTKVPKIKFVIIVSGAKFRSQSVAEKAYS
Sbjct: 121 LKHGPFDGFLGFSQGAILSAALPGLQAKGMALTKVPKIKFVIIVSGAKFRSQSVAEKAYS 180

Query: 181 PVIGCPSLHF----------------------------------LDDEGLEVMESFIEKI 216
             IGCPSLHF                                  LDD GLEVMESFIEKI
Sbjct: 181 SAIGCPSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDRGLEVMESFIEKI 240

BLAST of Carg08216 vs. NCBI nr
Match: XP_008444882.1 (PREDICTED: dihydrofolate reductase-like [Cucumis melo])

HSP 1 Score: 340.9 bits (873), Expect = 3.2e-90
Identity = 176/249 (70.68%), Postives = 190/249 (76.31%), Query Frame = 0

Query: 1   MSLLSPLLCSPISKSRSPEFHLRRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKW 60
           MS   P L S    S  P+FH   +PIMG+D +I  KPRFLCLHGFRTS AILKKQVGKW
Sbjct: 1   MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW 60

Query: 61  PDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYM 120
           P SVL+QLDL FLDAPFPAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM
Sbjct: 61  PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120

Query: 121 LKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYS 180
           ++HGPFDGFLGFSQGAILSAALPG QAKGIALTKVPKIKFVIIVSGAKFRS+SVAEKAYS
Sbjct: 121 IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS 180

Query: 181 PVIGCPSLHF----------------------------------LDDEGLEVMESFIEKI 216
             IGCPSLHF                                  LDD+ LEVMESFI++I
Sbjct: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI 240

BLAST of Carg08216 vs. NCBI nr
Match: XP_004152614.1 (PREDICTED: dihydrofolate reductase [Cucumis sativus] >KGN62729.1 hypothetical protein Csa_2G369800 [Cucumis sativus])

HSP 1 Score: 334.0 bits (855), Expect = 3.9e-88
Identity = 173/244 (70.90%), Postives = 186/244 (76.23%), Query Frame = 0

Query: 6   PLLCSPISKSRSPEFHLRRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVL 65
           P L S  S +  P+F    IPIMG+D +   KPRFLCLHGFRTS AILKKQVGKWP SVL
Sbjct: 6   PSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVL 65

Query: 66  EQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGP 125
           +QLDL FLDAPFPAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM+KHGP
Sbjct: 66  DQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGP 125

Query: 126 FDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGC 185
           FDGFLGFSQGAILSAALPG QAKGIALTKVPKIKFVIIVSGAKFRS+SVAEKAYS  IGC
Sbjct: 126 FDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGC 185

Query: 186 PSLHF----------------------------------LDDEGLEVMESFIEKISKMLD 216
           PSLHF                                  LDD+ LEVMESFI +ISK+L+
Sbjct: 186 PSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILN 245

BLAST of Carg08216 vs. TAIR10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process)

HSP 1 Score: 246.5 bits (628), Expect = 1.5e-65
Identity = 118/182 (64.84%), Postives = 144/182 (79.12%), Query Frame = 0

Query: 28  MGTDG-EIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIE 87
           MG++G  I RKPRFLCLHGFRTSG I+K Q+ KWP SV+++LDL FLDAPFP +GKSD+E
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 88  GIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ 147
           GIFDPPY+EWFQF+ EFTEY NF++CL ++ED M+K GPFDG +GFSQGAILS  LPGLQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 148 AKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHFLDDE------GLEVME 203
           AKGIA  KVPKIKFVII+ GAK +S  +AE AYS  +   SLHFL +       G +++E
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLGETDFLKPYGTQLIE 180

BLAST of Carg08216 vs. TAIR10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 237.7 bits (605), Expect = 6.9e-63
Identity = 113/206 (54.85%), Postives = 148/206 (71.84%), Query Frame = 0

Query: 12  ISKSRSPEFHLRRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLD 71
           ++ + +PE HL+            + PR LCLHGFRTSG IL+  +GKWPD++L  LDLD
Sbjct: 15  LTTAMAPEIHLK-----------PKNPRILCLHGFRTSGRILQAGIGKWPDTILRDLDLD 74

Query: 72  FLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLG 131
           FLDAPFPA GKSD+E  FDPPY+EW+Q +  F EYRNF+ECL++IEDYM+K+GPFDG LG
Sbjct: 75  FLDAPFPATGKSDVERFFDPPYYEWYQANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLG 134

Query: 132 FSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAK-----FRSQSVAEKAYSPVIGCP 191
           FSQGA L+AA+PG+Q +G ALTKVPK+KF++I+SGAK     F     A  A+S  + CP
Sbjct: 135 FSQGAFLTAAIPGMQEQGSALTKVPKVKFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCP 194

Query: 192 SLHFLDD------EGLEVMESFIEKI 207
           SLHF+ +      EG  ++ESF+E +
Sbjct: 195 SLHFIGERDFLKIEGEVLVESFVEPV 209

BLAST of Carg08216 vs. TAIR10
Match: AT1G09280.1 (Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645))

HSP 1 Score: 75.5 bits (184), Expect = 4.5e-14
Identity = 58/183 (31.69%), Postives = 83/183 (45.36%), Query Frame = 0

Query: 35  RRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPF----------PAEGKSD 94
           RRK R LCLHGFR + +  K + G     +    +L F+DAP           P  G  +
Sbjct: 351 RRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCN 410

Query: 95  ------IEGIFDPPYFEWF-----QFSP--EFTEYRNFDECLSFIEDYMLKHGPFDGFLG 154
                 +   FD P    +     QF P    T+   FD+ L++++    + GPFDG LG
Sbjct: 411 KKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILG 470

Query: 155 FSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHFL 195
           FSQGA ++AA+ G Q +   L      +F ++ SG  F    + E      I CPSLH  
Sbjct: 471 FSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSLHIF 528

BLAST of Carg08216 vs. Swiss-Prot
Match: sp|P36591|DYR_SCHPO (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 79.0 bits (193), Expect = 7.4e-14
Identity = 53/167 (31.74%), Postives = 80/167 (47.90%), Query Frame = 0

Query: 39  RFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSD------------IE 98
           + LCLHG+  SG +  K++G     + +  +L F   P  A+ ++D            + 
Sbjct: 6   KVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEXXXXRLAALG 65

Query: 99  GIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ 158
           G  +   F WF+       Y ++DE L  I  YM + GPFDG +GFSQGA + A L  + 
Sbjct: 66  GEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAMLAQML 125

Query: 159 AKG---IALTKVPKIKFVIIVSGAKFRSQSVA-EKAYSPVIGCPSLH 190
             G       + P  KFV+ V G  FR++    +  Y+P +  PSLH
Sbjct: 126 QPGQPPNPYVQHPPFKFVVFVGG--FRAEKPEFDHFYNPKLTTPSLH 170

BLAST of Carg08216 vs. Swiss-Prot
Match: sp|Q94AC1|STR6_ARATH (Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=STR6 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 8.1e-13
Identity = 58/183 (31.69%), Postives = 83/183 (45.36%), Query Frame = 0

Query: 35  RRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPF----------PAEGKSD 94
           RRK R LCLHGFR + +  K + G     +    +L F+DAP           P  G  +
Sbjct: 351 RRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCN 410

Query: 95  ------IEGIFDPPYFEWF-----QFSP--EFTEYRNFDECLSFIEDYMLKHGPFDGFLG 154
                 +   FD P    +     QF P    T+   FD+ L++++    + GPFDG LG
Sbjct: 411 KKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILG 470

Query: 155 FSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHFL 195
           FSQGA ++AA+ G Q +   L      +F ++ SG  F    + E      I CPSLH  
Sbjct: 471 FSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSLHIF 528

BLAST of Carg08216 vs. Swiss-Prot
Match: sp|Q9VDL1|LOVG_DROME (Esterase CG5412 OS=Drosophila melanogaster OX=7227 GN=CG5412 PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.0e-10
Identity = 57/192 (29.69%), Postives = 85/192 (44.27%), Query Frame = 0

Query: 33  EIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEG-KSDIEGIFDP 92
           EI  K R LCLHG+R +G   K ++G +     +  +  F+ AP  A+  +S  E +  P
Sbjct: 26  EITEKVRVLCLHGYRQNGEAFKNKLGSFRKFANKYAEFVFITAPHVAKALESAAEPV--P 85

Query: 93  PYFEW--------FQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALP 152
               W        F+ + +      F E L  +E+     GPF G LGFSQGA     + 
Sbjct: 86  EQRSWWANKDDGSFKGTNKGGPAFGFQESLRCVEEAWRTQGPFQGLLGFSQGACFVGLIC 145

Query: 153 GLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAE-KAYSPVIGCPSLHFLDDEGLEVMESF 212
           GL  K   LT + + +F ++ SG  F S S+    AY   I  P+LH        + +  
Sbjct: 146 GLAKK--KLTSI-RPEFAVLASG--FLSGSLVHMSAYEEAISIPTLHIYGQTDEIIPKEM 205

Query: 213 IEKISKMLDEAE 215
            E ++     AE
Sbjct: 206 SESLAARFKNAE 210

BLAST of Carg08216 vs. Swiss-Prot
Match: sp|Q3SZ07|OVCA2_BOVIN (Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.7e-10
Identity = 54/172 (31.40%), Postives = 73/172 (42.44%), Query Frame = 0

Query: 39  RFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGI---------F 98
           R LCL GFR S    +++ G    ++  + +L  L  P P    +  EG           
Sbjct: 8   RILCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVVDAAGSEGARPDSGPCPPE 67

Query: 99  DPPYFEWFQFS--------PEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAA 158
           + P   WF            E T  R  +E L  +   + K GPFDG LGFSQGA L+A 
Sbjct: 68  EQPQGWWFSEQEADVFLALEEPTACRGLEEALETVAQALNKLGPFDGILGFSQGAALAAL 127

Query: 159 LPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHFLDD 194
           +  L   G    + P  +FVI+VSG   R   + E      +  PSLH   D
Sbjct: 128 VCALGQGGD--PRFPLPRFVILVSGFCPRGLGLMEPIMQGPLSLPSLHVFGD 177

BLAST of Carg08216 vs. Swiss-Prot
Match: sp|Q29BR3|LOVG_DROPS (Esterase GA18864 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=GA18864 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.4e-09
Identity = 52/167 (31.14%), Postives = 75/167 (44.91%), Query Frame = 0

Query: 33  EIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAP-FPAEGKSDIEGIFDP 92
           EI  K R LCLHG+R  G   K ++G +     +  +  F+ AP   A  +S  E +  P
Sbjct: 26  EITEKVRVLCLHGYRQDGDAFKNKLGSFRKFTSKYAEFVFISAPHIAAPLESAAEPV--P 85

Query: 93  PYFEWFQFSPEFT--------EYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALP 152
               W+    + T            F + L  +E+     GPF G LGFSQGA     + 
Sbjct: 86  EQRSWWANKDDGTFKGTNKGGPAFGFQDSLRLVEEAWKTQGPFQGLLGFSQGACFVGLIC 145

Query: 153 GLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAE-KAYSPVIGCPSLH 190
           GL  K   LT + + +F ++ SG  F S S+    AY   +  P+LH
Sbjct: 146 GLAKK--KLTSI-RPEFAVLSSG--FVSGSLVHMSAYEEPVSIPTLH 185

BLAST of Carg08216 vs. TrEMBL
Match: tr|A0A1S3BC97|A0A1S3BC97_CUCME (dihydrofolate reductase-like OS=Cucumis melo OX=3656 GN=LOC103488098 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 2.1e-90
Identity = 176/249 (70.68%), Postives = 190/249 (76.31%), Query Frame = 0

Query: 1   MSLLSPLLCSPISKSRSPEFHLRRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKW 60
           MS   P L S    S  P+FH   +PIMG+D +I  KPRFLCLHGFRTS AILKKQVGKW
Sbjct: 1   MSSFFPSLSSHQFLSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKW 60

Query: 61  PDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYM 120
           P SVL+QLDL FLDAPFPAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM
Sbjct: 61  PPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYM 120

Query: 121 LKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYS 180
           ++HGPFDGFLGFSQGAILSAALPG QAKGIALTKVPKIKFVIIVSGAKFRS+SVAEKAYS
Sbjct: 121 IEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYS 180

Query: 181 PVIGCPSLHF----------------------------------LDDEGLEVMESFIEKI 216
             IGCPSLHF                                  LDD+ LEVMESFI++I
Sbjct: 181 TPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRI 240

BLAST of Carg08216 vs. TrEMBL
Match: tr|A0A0A0LNX4|A0A0A0LNX4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G369800 PE=4 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.6e-88
Identity = 173/244 (70.90%), Postives = 186/244 (76.23%), Query Frame = 0

Query: 6   PLLCSPISKSRSPEFHLRRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVL 65
           P L S  S +  P+F    IPIMG+D +   KPRFLCLHGFRTS AILKKQVGKWP SVL
Sbjct: 6   PSLSSHQSLTTIPQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVL 65

Query: 66  EQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGP 125
           +QLDL FLDAPFPAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM+KHGP
Sbjct: 66  DQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGP 125

Query: 126 FDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGC 185
           FDGFLGFSQGAILSAALPG QAKGIALTKVPKIKFVIIVSGAKFRS+SVAEKAYS  IGC
Sbjct: 126 FDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGC 185

Query: 186 PSLHF----------------------------------LDDEGLEVMESFIEKISKMLD 216
           PSLHF                                  LDD+ LEVMESFI +ISK+L+
Sbjct: 186 PSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILN 245

BLAST of Carg08216 vs. TrEMBL
Match: tr|A0A2N9IEM5|A0A2N9IEM5_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS50627 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.8e-73
Identity = 139/222 (62.61%), Postives = 167/222 (75.23%), Query Frame = 0

Query: 28  MGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEG 87
           MG++GE  RKPRFLCLHGFRTSG ILK QVGKWP+SVL+++DL FLDAPFP  GKSD+EG
Sbjct: 1   MGSEGETVRKPRFLCLHGFRTSGEILKTQVGKWPESVLQKIDLVFLDAPFPCLGKSDVEG 60

Query: 88  IFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQA 147
           IFDPPY+EWFQF+ EFTEY NFD+ L++IEDYM+KHGPFDGFLGFSQGAILSA LPGLQA
Sbjct: 61  IFDPPYYEWFQFNKEFTEYTNFDDSLAYIEDYMIKHGPFDGFLGFSQGAILSAGLPGLQA 120

Query: 148 KGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHF----------------- 207
           +G+AL KVPKIKF+II+ GAK +++SVAEKAYS  I C SLHF                 
Sbjct: 121 QGVALAKVPKIKFLIIIGGAKLKAESVAEKAYSSPIQCASLHFLGEKDFLKEYGKELLES 180

Query: 208 -----------------LDDEGLEVMESFIEKISKMLDEAEE 216
                            LD++GL++MESF+E+I +M +E EE
Sbjct: 181 CVDPVVIHHPKGHTIPRLDEKGLQIMESFLERIQEMNEEKEE 222

BLAST of Carg08216 vs. TrEMBL
Match: tr|A0A2C9WDZ0|A0A2C9WDZ0_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_02G147600 PE=4 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 2.0e-72
Identity = 139/221 (62.90%), Postives = 162/221 (73.30%), Query Frame = 0

Query: 28  MGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEG 87
           MG + E  RKPR LCLHGFRTSG ILKKQV KWP+SV+++LDL FLDAP+PA+GKSD+EG
Sbjct: 24  MGKEAEKVRKPRLLCLHGFRTSGEILKKQVHKWPESVVQKLDLVFLDAPYPAQGKSDVEG 83

Query: 88  IFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQA 147
           IF+PPY+EWFQF+ EFTEY NFDECL++IED+M+KHGPFDG LGFSQGAILSA LPGLQA
Sbjct: 84  IFEPPYYEWFQFNKEFTEYTNFDECLAYIEDFMIKHGPFDGLLGFSQGAILSAGLPGLQA 143

Query: 148 KGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHF----------------- 207
           KG+ALTKVPKIKF+II+ GAKF++ SVAEKAY   I CPSLHF                 
Sbjct: 144 KGVALTKVPKIKFLIIIGGAKFKATSVAEKAYGSPIQCPSLHFLGEMDFLKPYGLELLES 203

Query: 208 -----------------LDDEGLEVMESFIEKISKMLDEAE 215
                            LD++GLE   SFIEKI  ML + E
Sbjct: 204 CVDSVVVKHPKGHTIPRLDEQGLETTTSFIEKIENMLAKTE 244

BLAST of Carg08216 vs. TrEMBL
Match: tr|A0A1S2XM55|A0A1S2XM55_CICAR (UPF0483 protein AGAP003155 OS=Cicer arietinum OX=3827 GN=LOC101511725 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.3e-71
Identity = 128/182 (70.33%), Postives = 153/182 (84.07%), Query Frame = 0

Query: 31  DGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFD 90
           D  + RKPRFLCLHGFRTS  I+KKQ+ KWP  VL++LDL ++DAPFP  GKSD+EGIFD
Sbjct: 5   DNNMARKPRFLCLHGFRTSAEIMKKQIEKWPQDVLDKLDLVYVDAPFPCTGKSDVEGIFD 64

Query: 91  PPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGI 150
           PPY+EWFQF+ EFTEY NFDECL +IEDY++KHGPFDG LGFSQGAILSA++PGLQ KG+
Sbjct: 65  PPYYEWFQFNKEFTEYINFDECLQYIEDYIVKHGPFDGLLGFSQGAILSASIPGLQEKGV 124

Query: 151 ALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHFLDDE------GLEVMESFIE 207
           ALTKVPK+KFVII++GAKFRS SVAEKAYS +I CPSLHF+ +       G E+MESF+E
Sbjct: 125 ALTKVPKVKFVIIIAGAKFRSPSVAEKAYSSMITCPSLHFIGENDYLKQYGKELMESFVE 184

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022961839.17.8e-11385.54dihydrofolate reductase-like [Cucurbita moschata][more]
XP_023545459.12.8e-11083.94dihydrofolate reductase-like [Cucurbita pepo subsp. pepo][more]
XP_022997250.13.6e-11083.53dihydrofolate reductase-like [Cucurbita maxima][more]
XP_008444882.13.2e-9070.68PREDICTED: dihydrofolate reductase-like [Cucumis melo][more]
XP_004152614.13.9e-8870.90PREDICTED: dihydrofolate reductase [Cucumis sativus] >KGN62729.1 hypothetical pr... [more]
Match NameE-valueIdentityDescription
AT4G24380.11.5e-6564.84INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and deri... [more]
AT5G65400.16.9e-6354.85alpha/beta-Hydrolases superfamily protein[more]
AT1G09280.14.5e-1431.69Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645)[more]
Match NameE-valueIdentityDescription
sp|P36591|DYR_SCHPO7.4e-1431.74Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O... [more]
sp|Q94AC1|STR6_ARATH8.1e-1331.69Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=ST... [more]
sp|Q9VDL1|LOVG_DROME1.0e-1029.69Esterase CG5412 OS=Drosophila melanogaster OX=7227 GN=CG5412 PE=2 SV=1[more]
sp|Q3SZ07|OVCA2_BOVIN1.7e-1031.40Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1[more]
sp|Q29BR3|LOVG_DROPS1.4e-0931.14Esterase GA18864 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=GA18864 P... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3BC97|A0A1S3BC97_CUCME2.1e-9070.68dihydrofolate reductase-like OS=Cucumis melo OX=3656 GN=LOC103488098 PE=4 SV=1[more]
tr|A0A0A0LNX4|A0A0A0LNX4_CUCSA2.6e-8870.90Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G369800 PE=4 SV=1[more]
tr|A0A2N9IEM5|A0A2N9IEM5_FAGSY1.8e-7362.61Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS50627 PE=4 SV=1[more]
tr|A0A2C9WDZ0|A0A2C9WDZ0_MANES2.0e-7262.90Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_02G147600 PE=4 SV=... [more]
tr|A0A1S2XM55|A0A1S2XM55_CICAR1.3e-7170.33UPF0483 protein AGAP003155 OS=Cicer arietinum OX=3827 GN=LOC101511725 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR029058AB_hydrolase
IPR005645FSH_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08216-RACarg08216-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005645Serine hydrolase FSHPFAMPF03959FSH1coord: 36..191
e-value: 4.9E-38
score: 130.8
IPR029058Alpha/Beta hydrolase foldGENE3DG3DSA:3.40.50.1820coord: 34..214
e-value: 8.1E-40
score: 138.8
NoneNo IPR availablePANTHERPTHR22778OVARIAN CANCER GENE-2 PROTEIN-RELATEDcoord: 31..196
NoneNo IPR availablePANTHERPTHR22778:SF25SUBFAMILY NOT NAMEDcoord: 31..196

The following gene(s) are paralogous to this gene:

None