Carg08085 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg08085
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	GDSL esterase/lipase
Location	Cucurbita_argyrosperma_scaffold_050 : 629512 .. 631462 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAGAGAATTCAGAATCCAATTCCAATGCTAGAAATATTGGTCACTTCAGTTTGATAATTTTCGATGGGCGAATCCCAGTTTTGCAGAAGGATTCAATCTTATCCAAAACGAAGCTTCAATTTCACGACCAGACTGCTTAAAAGTGAAGATCAATCTGCGGAGAAATGGAGAAGAACAGACTATGAGGCCCGAGATCGTTCTATTTGGTGATTCTTTAACCGAGCAATCCTTCAGTGCAGGTGGATGGGGAGCTGCGCTTGCTGATACTTACTCTCGCAAGGTGAGTTTTCTATTGTTCCTTTTTATTTTGTATTTCACTCAATTTTTTTGAAGTTTTAGGGTTTGTAATTGGGATATTGTCTTCGTAATCGCAGGCGGATGTTCTTGTTCGTGGCTATGATGGTTACAATACTCGCTGGGCTTTGATCTTACTTCCTCATGTTTTCTCCTCGGTATTTGTTTTAGTGCTCTTTCTGTTCCTTGTCTGCTTTGATTTTGATGATTTCTTGGTAGGATATTGCCCTAAATTATTGTGGTACAGTCCTTAGGAGCTCGTTTTTTTGATGATTTTGATGATTTTGATGATTTCATTATTTGACTTCATACACATTGGTTATAGCTTTTGGTAGCCTGAAATTGAAGCATAGTCTCTTGAAGTCAGGTTTACAAGGCGTCGCTGTTAGTTTTTGTGTGTTTCCATGCTCCTTGGAGACTCTAACAATGAACCATTATGATCTAAATTTCGAAGTATCCTTTTTATGTTCTTTACACTAGCTTAGCTTACTTAACTCATGTGCAGAAACGAGCCCCTGCTGTGGTGACAGTTTTCTTTGGGGCCAATGATGCCACTATTTTGGGGGGAAGACTTGGGAGACTACACGTACCCGTTGAAGAGTATAAAGATAATCTTAAAACAATAGTAAATCATTTGAAGGTATTGATTCATTCATTGCTTGAAATCTTTTGCAGCAGAATGTTTTGACCTTTGTTTTGAAGATTTAAACTGAGTATCATATGGTAGTCTGTGTCTTTTATATTCTATCCTTGTTGGCCTGTTGTCTTTCACAATGAGTGTGAGATCCCACATAAGTTGGAGATAATAACAAAACATTCTTTGTGTCTTTTATATTCTATCCTTGTTGGCCTGTTGTCTTTCACAATGAGTGTGAGATCCCACATAAGTTGGAGATAATAACAAAACATTCTTTATGAGGATGTGGACGGAAGTCTGAAAGGGAAAGTTCAAAGAGGACAATATGTGCTGGTGGTGGAATTGGTTCGTTACAAATGGTATCAGAGTTAGACACCCGATGTGCCAGCGAGGACGTTGAGCCCCAAAGGGGGGTGGATTGCGAGATCGTACATCGATTGGAGACAGGAACGAGTATCAACGAGGATGCTGGGCCTCGAAGGGGGTGGATTCTGAGATCCCACATCGGTTGGAGAGGGGAACGAAACATTCTTTAAAAGGATTTGGAAACCTCTCCCTATCAAACACGTTTTAAAAACCTTGAGGAGAAGCCCGAAAGAGAAAACTTCAAAGAGGCCAATGTCTGATGTGTTTAGATAATAAACTATAACCATTTTGGCCTCCAATCTAAGATATTAAGCTATCATACGAGTTTGTCAAAACCAAATGTCGTAGGATAGGACGACACGGATGACTTATGAGAGAGATGTATTCGCTTGCTGACCCAAAACACCTAAAGTATTAGAAAGAAAACTGCAATCAATCTGAGAAACATTATGCTTCCATTTGTTTTGGGTTCCTCTGGTTCATCCTTCTCAATTCCTTTTGTAATTGGAGCACTCTTATTTTGGTTGCAGGAGTGCTACCCAACCACACTGGTTGTACTTATTACTCCTCCTCCTGTTGATGAGGAAGGTTGCAAGGAATATGCACGGTATCACTTTTCCATCTTCCTACTTTTCCTTCAACTAGTTTCATGA

mRNA sequence

TAAGAGAATTCAGAATCCAATTCCAATGCTAGAAATATTGGTCACTTCAGTTTGATAATTTTCGATGGGCGAATCCCAGTTTTGCAGAAGGATTCAATCTTATCCAAAACGAAGCTTCAATTTCACGACCAGACTGCTTAAAAGTGAAGATCAATCTGCGGAGAAATGGAGAAGAACAGACTATGAGGCCCGAGATCGTTCTATTTGGTGATTCTTTAACCGAGCAATCCTTCAGTGCAGGTGGATGGGGAGCTGCGCTTGCTGATACTTACTCTCGCAAGGCGGATGTTCTTGTTCGTGGCTATGATGGTTACAATACTCGCTGGGCTTTGATCTTACTTCCTCATGTTTTCTCCTCGGTATTTGTTTTAGTGCTCTTTCTGTTCCTTGTCTGCTTTGATTTTGATGATTTCTTGAAACGAGCCCCTGCTGTGGTGACAGTTTTCTTTGGGGCCAATGATGCCACTATTTTGGGGGGAAGACTTGGGAGACTACACGTACCCGTTGAAGAGTATAAAGATAATCTTAAAACAATAGTAAATCATTTGAAGGAGTGCTACCCAACCACACTGGTTGTACTTATTACTCCTCCTCCTGTTGATGAGGAAGGTTGCAAGGAATATGCACGGTATCACTTTTCCATCTTCCTACTTTTCCTTCAACTAGTTTCATGA

Coding sequence (CDS)

ATGAGGCCCGAGATCGTTCTATTTGGTGATTCTTTAACCGAGCAATCCTTCAGTGCAGGTGGATGGGGAGCTGCGCTTGCTGATACTTACTCTCGCAAGGCGGATGTTCTTGTTCGTGGCTATGATGGTTACAATACTCGCTGGGCTTTGATCTTACTTCCTCATGTTTTCTCCTCGGTATTTGTTTTAGTGCTCTTTCTGTTCCTTGTCTGCTTTGATTTTGATGATTTCTTGAAACGAGCCCCTGCTGTGGTGACAGTTTTCTTTGGGGCCAATGATGCCACTATTTTGGGGGGAAGACTTGGGAGACTACACGTACCCGTTGAAGAGTATAAAGATAATCTTAAAACAATAGTAAATCATTTGAAGGAGTGCTACCCAACCACACTGGTTGTACTTATTACTCCTCCTCCTGTTGATGAGGAAGGTTGCAAGGAATATGCACGGTATCACTTTTCCATCTTCCTACTTTTCCTTCAACTAGTTTCATGA

Protein sequence

MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSVFVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVNHLKECYPTTLVVLITPPPVDEEGCKEYARYHFSIFLLFLQLVS

Homology

[Close]

BLAST of Carg08085 vs. NCBI nr
Match: XP_022941566.1 (GDSL esterase/lipase At5g62930-like [Cucurbita moschata])

HSP 1 Score: 256.1 bits (653), Expect = 7.8e-65
Identity = 128/149 (85.91%), Postives = 129/149 (86.58%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSS 
Sbjct: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSS- 60

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
                             KRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN
Sbjct: 61  ------------------KRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120

Query: 121 HLKECYPTTLVVLITPPPVDEEGCKEYAR 150
           HLKECYPT+LVVLITPPPVDEEG KEYAR
Sbjct: 121 HLKECYPTSLVVLITPPPVDEEGPKEYAR 130

[Close]

BLAST of Carg08085 vs. NCBI nr
Match: XP_022991514.1 (GDSL esterase/lipase At5g62930-like [Cucurbita maxima])

HSP 1 Score: 251.5 bits (641), Expect = 1.9e-63
Identity = 125/149 (83.89%), Postives = 127/149 (85.23%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSS 
Sbjct: 36  MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSS- 95

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
                             K+ P VVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN
Sbjct: 96  ------------------KQGPVVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 155

Query: 121 HLKECYPTTLVVLITPPPVDEEGCKEYAR 150
           HLKECYPT+LVVLITPPPVDEEG KEYAR
Sbjct: 156 HLKECYPTSLVVLITPPPVDEEGRKEYAR 165

[Close]

BLAST of Carg08085 vs. NCBI nr
Match: XP_023523011.1 (GDSL esterase/lipase At5g62930-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 251.1 bits (640), Expect = 2.5e-63
Identity = 125/149 (83.89%), Postives = 126/149 (84.56%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSS 
Sbjct: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSS- 60

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
                             KRAP VVTVFFGANDATI GGRLGRLHVPVEEYKDNLK IVN
Sbjct: 61  ------------------KRAPVVVTVFFGANDATIFGGRLGRLHVPVEEYKDNLKAIVN 120

Query: 121 HLKECYPTTLVVLITPPPVDEEGCKEYAR 150
           HLKECYPT+LVVLITPPPVDEEG KEYAR
Sbjct: 121 HLKECYPTSLVVLITPPPVDEEGRKEYAR 130

[Close]

BLAST of Carg08085 vs. NCBI nr
Match: XP_022149344.1 (GDSL esterase/lipase At5g62930 isoform X1 [Momordica charantia])

HSP 1 Score: 205.7 bits (522), Expect = 1.2e-49
Identity = 102/149 (68.46%), Postives = 115/149 (77.18%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           MRP+IVLFGDSLT QSF +GGWGAALADTYSRKADV+VRGY GYN+RWAL LL H+F   
Sbjct: 1   MRPQIVLFGDSLTAQSFGSGGWGAALADTYSRKADVVVRGYGGYNSRWALFLLHHIFP-- 60

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
                   LVC D    + + P VVT+FFGANDA ILG    R HVP+EE+K+NLK +VN
Sbjct: 61  --------LVCAD----IPKPPIVVTIFFGANDAAILGRTSERQHVPLEEFKNNLKKMVN 120

Query: 121 HLKECYPTTLVVLITPPPVDEEGCKEYAR 150
           HLKEC PTTLV+LITPPPVDEEG  EYAR
Sbjct: 121 HLKECSPTTLVILITPPPVDEEGRNEYAR 135

[Close]

BLAST of Carg08085 vs. NCBI nr
Match: XP_022922525.1 (GDSL esterase/lipase At5g62930 [Cucurbita moschata])

HSP 1 Score: 200.7 bits (509), Expect = 3.9e-48
Identity = 100/149 (67.11%), Postives = 110/149 (73.83%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           MRPEIVLFGDSLT QSF +GGWGAALADTYSRKADVLVRGY GYNTRWAL LL H+F   
Sbjct: 1   MRPEIVLFGDSLTAQSFGSGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL- 60

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
                            + +AP VVT+FFGANDA I G    R HVP++EYKDNLK +VN
Sbjct: 61  ----------------DIPKAPIVVTIFFGANDAAIFGRTSERQHVPLDEYKDNLKKMVN 120

Query: 121 HLKECYPTTLVVLITPPPVDEEGCKEYAR 150
           HLKE  PTTLV+LITPPPVDE+G  EYAR
Sbjct: 121 HLKEYSPTTLVILITPPPVDEDGRNEYAR 132

[Close]

BLAST of Carg08085 vs. TAIR10
Match: AT5G62930.1 (SGNH hydrolase-type esterase superfamily protein)

HSP 1 Score: 178.7 bits (452), Expect = 2.9e-45
Identity = 87/148 (58.78%), Postives = 100/148 (67.57%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           MRPEIVLFGDS+T QSF +GGWG+ALAD YSRKADV+VRGY GYNTRWAL LL H+F   
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPL- 60

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
                                P   T+FFGANDA + G    R HVPVEEY DN++ IV 
Sbjct: 61  ----------------GSSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQ 120

Query: 121 HLKECYPTTLVVLITPPPVDEEGCKEYA 149
           HLK+C PT L+VLITPPP+DE G + YA
Sbjct: 121 HLKKCSPTMLIVLITPPPIDEAGRQSYA 131

[Close]

BLAST of Carg08085 vs. TAIR10
Match: AT5G45920.1 (SGNH hydrolase-type esterase superfamily protein)

HSP 1 Score: 148.3 bits (373), Expect = 4.2e-36
Identity = 75/147 (51.02%), Postives = 96/147 (65.31%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           MR +I LFGDS+TE+SFS GGWGA+LAD   RKAD+++RGY GYNTRWAL ++  VF   
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
                         ++    +PA VTVFFGANDA +     G  HVP+ EYK NL++IV+
Sbjct: 62  --------------EEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVS 121

Query: 121 HLKECYPTTLVVLITPPPVDEEGCKEY 148
            LK  +P T ++LITPPP+DEE    Y
Sbjct: 122 FLKNRWPQTAIILITPPPIDEEARLRY 134

[Close]

BLAST of Carg08085 vs. TAIR10
Match: AT2G38180.1 (SGNH hydrolase-type esterase superfamily protein)

HSP 1 Score: 109.0 bits (271), Expect = 2.8e-24
Identity = 57/142 (40.14%), Postives = 84/142 (59.15%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           +RP+IVLFG S+ + SF+  GWGA LAD YSR AD+++RGY G+N+R+AL +L  VF   
Sbjct: 5   VRPQIVLFGSSIVQYSFTDRGWGATLADLYSRTADIILRGYAGWNSRFALKVLHQVFPKD 64

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
            V+                  P++V V+FG ND+T         HVP+ E+ +N++ I  
Sbjct: 65  AVI-----------------QPSLVIVYFGGNDSTHPHPSGHGPHVPLSEFIENMRKIGE 124

Query: 121 HLKECYPTTLVVLITPPPVDEE 143
           HL      T V+ +TPPP++E+
Sbjct: 125 HLLSLSDKTRVIFLTPPPMNEK 129

[Close]

BLAST of Carg08085 vs. TAIR10
Match: AT3G11210.1 (SGNH hydrolase-type esterase superfamily protein)

HSP 1 Score: 104.4 bits (259), Expect = 6.9e-23
Identity = 54/140 (38.57%), Postives = 79/140 (56.43%), Query Frame = 0


Query: 2   RPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSVF 61
           RP+IVLFG S+ + SF  GGWGA L++ Y+RKAD+++RGY G+N+  AL ++  VF    
Sbjct: 6   RPQIVLFGSSIVQMSFGHGGWGAILSEVYARKADIILRGYYGWNSSRALEVVDQVFPKDA 65

Query: 62  VLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVNH 121
            +                  P++V V+FG ND+          HVP+ EY DN+K I  H
Sbjct: 66  AV-----------------QPSLVIVYFGGNDSMAPHSSGLGPHVPLTEYVDNMKKIALH 125

Query: 122 LKECYPTTLVVLITPPPVDE 142
           L+     T ++ ++ PPVDE
Sbjct: 126 LQSLSDFTRIIFLSSPPVDE 128

[Close]

BLAST of Carg08085 vs. Swiss-Prot
Match: sp|Q9FM04|GDL88_ARATH (GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2 SV=3)

HSP 1 Score: 178.7 bits (452), Expect = 5.2e-44
Identity = 87/148 (58.78%), Postives = 100/148 (67.57%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           MRPEIVLFGDS+T QSF +GGWG+ALAD YSRKADV+VRGY GYNTRWAL LL H+F   
Sbjct: 1   MRPEIVLFGDSITAQSFRSGGWGSALADAYSRKADVVVRGYGGYNTRWALFLLHHIFPL- 60

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
                                P   T+FFGANDA + G    R HVPVEEY DN++ IV 
Sbjct: 61  ----------------GSSSPPVATTIFFGANDAALKGRTSDRQHVPVEEYTDNVRKIVQ 120

Query: 121 HLKECYPTTLVVLITPPPVDEEGCKEYA 149
           HLK+C PT L+VLITPPP+DE G + YA
Sbjct: 121 HLKKCSPTMLIVLITPPPIDEAGRQSYA 131

[Close]

BLAST of Carg08085 vs. Swiss-Prot
Match: sp|Q6NMR9|GDL84_ARATH (GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 7.5e-35
Identity = 75/147 (51.02%), Postives = 96/147 (65.31%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           MR +I LFGDS+TE+SFS GGWGA+LAD   RKAD+++RGY GYNTRWAL ++  VF   
Sbjct: 2   MRRKIFLFGDSITEESFSDGGWGASLADLLRRKADMVLRGYSGYNTRWALKVVERVFPVA 61

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
                         ++    +PA VTVFFGANDA +     G  HVP+ EYK NL++IV+
Sbjct: 62  --------------EEDGGDSPAAVTVFFGANDACLPERCSGFQHVPLHEYKQNLRSIVS 121

Query: 121 HLKECYPTTLVVLITPPPVDEEGCKEY 148
            LK  +P T ++LITPPP+DEE    Y
Sbjct: 122 FLKNRWPQTAIILITPPPIDEEARLRY 134

[Close]

BLAST of Carg08085 vs. Swiss-Prot
Match: sp|Q2TAA2|IAH1_HUMAN (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Homo sapiens OX=9606 GN=IAH1 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 8.3e-26
Identity = 65/145 (44.83%), Postives = 85/145 (58.62%), Query Frame = 0


Query: 3   PEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSVFV 62
           P ++LFGDS+T+ SF  GGWGA+LAD   RK DVL RG+ GYNTRWA I+LP +      
Sbjct: 16  PRLLLFGDSITQFSFQQGGWGASLADRLVRKCDVLNRGFSGYNTRWAKIILPRLIRKGNS 75

Query: 63  LVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVNHL 122
           L +                P  VT+FFGAND+  L     + H+P+EEY  NLK++V +L
Sbjct: 76  LDI----------------PVAVTIFFGANDSA-LKDENPKQHIPLEEYAANLKSMVQYL 135

Query: 123 KEC-YPTTLVVLITPPPVDEEGCKE 147
           K    P   V+LITP P+ E   +E
Sbjct: 136 KSVDIPENRVILITPTPLCETAWEE 143

[Close]

BLAST of Carg08085 vs. Swiss-Prot
Match: sp|Q711G3|IAH1_RAT (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Rattus norvegicus OX=10116 GN=Iah1 PE=2 SV=2)

HSP 1 Score: 117.9 bits (294), Expect = 1.1e-25
Identity = 66/153 (43.14%), Postives = 85/153 (55.56%), Query Frame = 0


Query: 3   PEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSVFV 62
           P ++LFGDS+T+ SF  GGWG  LAD   RK DVL RG+ GYNTRWA I+LP +      
Sbjct: 16  PRVLLFGDSITQFSFQQGGWGTLLADRLVRKCDVLNRGFSGYNTRWAKIILPRIIRKGAG 75

Query: 63  LVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVNHL 122
           L                  P  VT+FFGAND+T L     + HVP++EY  NL+ +V +L
Sbjct: 76  L----------------ENPVAVTIFFGANDST-LKDENPKQHVPLDEYSANLRDMVQYL 135

Query: 123 KEC-YPTTLVVLITPPPVDE---------EGCK 146
           +    P   V+LITPPP+ E         +GCK
Sbjct: 136 RSVDIPKERVILITPPPLCEAAWEKECILKGCK 151

[Close]

BLAST of Carg08085 vs. Swiss-Prot
Match: sp|Q3SZ16|IAH1_BOVIN (Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Bos taurus OX=9913 GN=IAH1 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.9e-25
Identity = 69/166 (41.57%), Postives = 92/166 (55.42%), Query Frame = 0


Query: 3   PEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSVFV 62
           P ++LFGDS+T+ SF  GGWGA+LAD   RK DVL RG+ GYNTRWA I+LP +      
Sbjct: 16  PRVLLFGDSITQFSFQQGGWGASLADMLVRKCDVLNRGFSGYNTRWAKIILPRLVRKGSG 75

Query: 63  LVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVNHL 122
           L                 +P  VT+FFGAND+  L     + HVP+EE+  NL+++V +L
Sbjct: 76  L----------------DSPVAVTIFFGANDSA-LKDENPKQHVPLEEFVANLRSMVRYL 135

Query: 123 KEC-YPTTLVVLITPPPVDE---------EGCK---------EYAR 150
           +    P   ++LITPPP+ E         +GCK         EYAR
Sbjct: 136 RSVDVPEGRLILITPPPLCEAAWAQECLQQGCKLNRLNSVVGEYAR 164

[Close]

BLAST of Carg08085 vs. TrEMBL
Match: tr|A0A1S3BZF4|A0A1S3BZF4_CUCME (GDSL esterase/lipase At5g62930 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494719 PE=4 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.3e-47
Identity = 102/149 (68.46%), Postives = 108/149 (72.48%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           MRPEIVLFGDS+T QSF  GGWGAALADTYSRKADVLVRGY GYNTRWAL LL H+F   
Sbjct: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVLVRGYGGYNTRWALFLLHHIFPL- 60

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
                              +AP VVT+FFGANDA ILG    R HVP+EEYK NLK +VN
Sbjct: 61  ----------------NSPKAPIVVTIFFGANDAAILGRTSERQHVPLEEYKYNLKKMVN 120

Query: 121 HLKECYPTTLVVLITPPPVDEEGCKEYAR 150
           HLKE  PTTLVVLITPPPVDEEG  EYAR
Sbjct: 121 HLKEYSPTTLVVLITPPPVDEEGRNEYAR 132

[Close]

BLAST of Carg08085 vs. TrEMBL
Match: tr|A0A2K1YAR5|A0A2K1YAR5_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_012G081900v3 PE=4 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 6.3e-47
Identity = 97/155 (62.58%), Postives = 111/155 (71.61%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           MRP+IVLFGDS+TEQSF +GGWG++LA+TYSRKADVLVRGY GYNTRWAL LL H+F   
Sbjct: 1   MRPQIVLFGDSITEQSFRSGGWGSSLANTYSRKADVLVRGYGGYNTRWALFLLTHIFPLN 60

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
                              + PA  T+FFGANDA +LG    R HVPVEEYK+NLK +V 
Sbjct: 61  ST-----------------KPPAATTIFFGANDAALLGRNSERQHVPVEEYKENLKKMVL 120

Query: 121 HLKECYPTTLVVLITPPPVDEEGCKEYARYHFSIF 156
           HLKEC P  LVVLITPPPVDEEG KEYA+Y   +F
Sbjct: 121 HLKECSPAMLVVLITPPPVDEEGRKEYAKYDPFLF 138

[Close]

BLAST of Carg08085 vs. TrEMBL
Match: tr|A0A0A0KTH4|A0A0A0KTH4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G004790 PE=4 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 8.3e-47
Identity = 98/149 (65.77%), Postives = 108/149 (72.48%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           MRPEIVLFGDS+T QSF  GGWGAALADTYSRKADV+VRGY GYNTRWAL LL H+F   
Sbjct: 1   MRPEIVLFGDSITAQSFGLGGWGAALADTYSRKADVIVRGYGGYNTRWALFLLHHIFPL- 60

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
                              +AP VVT+FFGANDA +LG    + HVP+EEYK NLK +VN
Sbjct: 61  ----------------NSPKAPIVVTIFFGANDAAVLGRTSEKQHVPLEEYKYNLKKMVN 120

Query: 121 HLKECYPTTLVVLITPPPVDEEGCKEYAR 150
           HLKE  PTTLV+LITPPPVDEEG  EYAR
Sbjct: 121 HLKEYSPTTLVILITPPPVDEEGRNEYAR 132

[Close]

BLAST of Carg08085 vs. TrEMBL
Match: tr|M5WBP3|M5WBP3_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G172500 PE=4 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.1e-46
Identity = 97/149 (65.10%), Postives = 108/149 (72.48%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           MRPEIVLFGDS+TEQSF +GGWGAALADTYSRKADV VRGY GYNTRWAL L+ H+    
Sbjct: 1   MRPEIVLFGDSITEQSFRSGGWGAALADTYSRKADVKVRGYGGYNTRWALFLMQHL---- 60

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
                      F  D   K+ PA  T+FFGANDA ILG    R HVPVEEYK+NL+ IV 
Sbjct: 61  -----------FPLDS--KKPPAAATIFFGANDAAILGRTSERQHVPVEEYKENLRKIVL 120

Query: 121 HLKECYPTTLVVLITPPPVDEEGCKEYAR 150
           H+KEC P  L+VLITPPPVDE+G  EYAR
Sbjct: 121 HMKECSPAILIVLITPPPVDEDGRNEYAR 132

[Close]

BLAST of Carg08085 vs. TrEMBL
Match: tr|A0A1R3HBB5|A0A1R3HBB5_9ROSI (Lipase, GDSL OS=Corchorus olitorius OX=93759 GN=COLO4_30055 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.4e-46
Identity = 97/149 (65.10%), Postives = 106/149 (71.14%), Query Frame = 0


Query: 1   MRPEIVLFGDSLTEQSFSAGGWGAALADTYSRKADVLVRGYDGYNTRWALILLPHVFSSV 60
           MRP IVLFGDS+TEQSF  GGWGA+LADTYSRKADVLVRGY GYNTRWAL LL H+    
Sbjct: 1   MRPNIVLFGDSITEQSFRPGGWGASLADTYSRKADVLVRGYGGYNTRWALFLLHHL---- 60

Query: 61  FVLVLFLFLVCFDFDDFLKRAPAVVTVFFGANDATILGGRLGRLHVPVEEYKDNLKTIVN 120
                      F  D   K+ P   T+FFGANDA +LG    R HVPVEEYK+NL  IV 
Sbjct: 61  -----------FPLDS--KKHPLATTIFFGANDAALLGRNSERQHVPVEEYKENLSKIVK 120

Query: 121 HLKECYPTTLVVLITPPPVDEEGCKEYAR 150
           HLKEC PT L+VLITPPP+DEEG  EYAR
Sbjct: 121 HLKECSPTMLIVLITPPPIDEEGRMEYAR 132

The following BLAST results are available for this feature:

BLAST of Carg08085 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022941566.1	7.8e-65	85.91	GDSL esterase/lipase At5g62930-like [Cucurbita moschata]	[more]
XP_022991514.1	1.9e-63	83.89	GDSL esterase/lipase At5g62930-like [Cucurbita maxima]	[more]
XP_023523011.1	2.5e-63	83.89	GDSL esterase/lipase At5g62930-like [Cucurbita pepo subsp. pepo]	[more]
XP_022149344.1	1.2e-49	68.46	GDSL esterase/lipase At5g62930 isoform X1 [Momordica charantia]	[more]
XP_022922525.1	3.9e-48	67.11	GDSL esterase/lipase At5g62930 [Cucurbita moschata]	[more]

BLAST of Carg08085 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G62930.1	2.9e-45	58.78	SGNH hydrolase-type esterase superfamily protein	[more]
AT5G45920.1	4.2e-36	51.02	SGNH hydrolase-type esterase superfamily protein	[more]
AT2G38180.1	2.8e-24	40.14	SGNH hydrolase-type esterase superfamily protein	[more]
AT3G11210.1	6.9e-23	38.57	SGNH hydrolase-type esterase superfamily protein	[more]

BLAST of Carg08085 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9FM04\|GDL88_ARATH	5.2e-44	58.78	GDSL esterase/lipase At5g62930 OS=Arabidopsis thaliana OX=3702 GN=At5g62930 PE=2...	[more]
sp\|Q6NMR9\|GDL84_ARATH	7.5e-35	51.02	GDSL esterase/lipase At5g45920 OS=Arabidopsis thaliana OX=3702 GN=At5g45920 PE=2...	[more]
sp\|Q2TAA2\|IAH1_HUMAN	8.3e-26	44.83	Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Homo sapiens OX=9606 GN=IAH1 P...	[more]
sp\|Q711G3\|IAH1_RAT	1.1e-25	43.14	Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Rattus norvegicus OX=10116 GN=...	[more]
sp\|Q3SZ16\|IAH1_BOVIN	1.9e-25	41.57	Isoamyl acetate-hydrolyzing esterase 1 homolog OS=Bos taurus OX=9913 GN=IAH1 PE=...	[more]

BLAST of Carg08085 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BZF4\|A0A1S3BZF4_CUCME	1.3e-47	68.46	GDSL esterase/lipase At5g62930 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349471...	[more]
tr\|A0A2K1YAR5\|A0A2K1YAR5_POPTR	6.3e-47	62.58	Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_012G081900v3 PE=...	[more]
tr\|A0A0A0KTH4\|A0A0A0KTH4_CUCSA	8.3e-47	65.77	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G004790 PE=4 SV=1	[more]
tr\|M5WBP3\|M5WBP3_PRUPE	1.1e-46	65.10	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G172500 PE=4 SV=1	[more]
tr\|A0A1R3HBB5\|A0A1R3HBB5_9ROSI	1.4e-46	65.10	Lipase, GDSL OS=Corchorus olitorius OX=93759 GN=COLO4_30055 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR013830	SGNH_hydro
IPR036514	SGNH_hydro_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08085-RA	Carg08085-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036514	SGNH hydrolase superfamily	GENE3D	G3DSA:3.40.50.1110		coord: 2..153 e-value: 7.7E-43 score: 148.4
IPR013830	SGNH hydrolase-type esterase domain	PFAM	PF13472	Lipase_GDSL_2	coord: 7..143 e-value: 1.2E-14 score: 55.0
None	No IPR available	PANTHER	PTHR45192	FAMILY NOT NAMED	coord: 1..151
None	No IPR available	SUPERFAMILY	SSF52266	SGNH hydrolase	coord: 3..143

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg08085	Cla006491	Watermelon (97103) v1	carwmB0366
Carg08085	CmoCh16G003000	Cucurbita moschata (Rifu)	carcmoB0509
Carg08085	Lsi03G009810	Bottle gourd (USVL1VR-Ls)	carlsiB341
Carg08085	Cp4.1LG14g04870	Cucurbita pepo (Zucchini)	carcpeB0523

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg08085	Silver-seed gourd	carcarB324
Carg08085	Silver-seed gourd	carcarB404
Carg08085	Cucumber (Gy14) v2	carcgybB0385
Carg08085	Cucumber (Gy14) v1	carcgyB0435
Carg08085	Cucumber (Gy14) v1	carcgyB0440
Carg08085	Cucurbita maxima (Rimu)	carcmaB0528
Carg08085	Cucurbita maxima (Rimu)	carcmaB0525
Carg08085	Cucurbita maxima (Rimu)	carcmaB0526
Carg08085	Cucurbita maxima (Rimu)	carcmaB0529
Carg08085	Cucurbita moschata (Rifu)	carcmoB0508
Carg08085	Cucurbita moschata (Rifu)	carcmoB0513
Carg08085	Cucurbita moschata (Rifu)	carcmoB0514
Carg08085	Cucurbita pepo (Zucchini)	carcpeB0526
Carg08085	Cucurbita pepo (Zucchini)	carcpeB0527
Carg08085	Cucurbita pepo (Zucchini)	carcpeB0529
Carg08085	Wild cucumber (PI 183967)	carcpiB0410
Carg08085	Wild cucumber (PI 183967)	carcpiB0414
Carg08085	Cucumber (Chinese Long) v2	carcuB0405
Carg08085	Cucumber (Chinese Long) v2	carcuB0411
Carg08085	Cucumber (Chinese Long) v3	carcucB0408
Carg08085	Cucumber (Chinese Long) v3	carcucB0413
Carg08085	Melon (DHL92) v3.5.1	carmeB0378
Carg08085	Melon (DHL92) v3.5.1	carmeB0382
Carg08085	Melon (DHL92) v3.5.1	carmeB0383
Carg08085	Melon (DHL92) v3.6.1	carmedB0383
Carg08085	Melon (DHL92) v3.6.1	carmedB0386
Carg08085	Melon (DHL92) v3.6.1	carmedB0387
Carg08085	Watermelon (Charleston Gray)	carwcgB0364
Carg08085	Watermelon (Charleston Gray)	carwcgB0365
Carg08085	Watermelon (97103) v2	carwmbB0391
Carg08085	Watermelon (97103) v2	carwmbB0402
Carg08085	Wax gourd	carwgoB0456