Carg07589 (gene) Silver-seed gourd

NameCarg07589
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description4-coumarate-CoA ligase
LocationCucurbita_argyrosperma_scaffold_024 : 459926 .. 463689 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCTGTTTTGGAATTTCCTATTGGTTGATTGTGCAATTTATTATTATTTTAATCTTGTAAAGTTGTTTTTGTTTTTTTAATCGGTTTGAATATTCAATCCAAAACATTCTCAACTAATTTGAATAAAATAAGTTGGATCAGCGTCCGCGTGCTCGATCGCGCATCACTTTCTTCGAGTCCACTTCCATGGCAGATCGGAATCCCAGCTTAAACGCAGCCCATTTCGTTGATCATCGAAGCGGTTTCTGTCCTCAGACGAAGATTTTCCATAGCCTCCGACCACCTCTCTTAATTCCGCCATTATCTCAGCCTCTCTCCGTTACCGAACACGCCCTTTCTCTCCTTCAATCGTCCCCTCCTCCTTCAAATGCCGCCGTTCTCGTCGACTCCAACTCTGGTGTGCATCTCTCCTATGCCATTTTCCTCCGTCAAATACGAACACTCGCCTCTAATCTTAAAGCGTTAACCTCACTCTCCAATGGTCAAGTGGCTTTTATTCTCGCACCGACTTCTTTGGAAGTCCCTGTGTTGTATTTTGCTCTGTTATCTCTCGGTGTTGTTATCTCTCCGGCTAATCCGATTGGTTCCGATTCGGAGATTTCTCATCAGATTCGACTCAGTGAACCGGTCATTGCCTTCGCTACATCCTCGACGGCGTCCAAGCTCCCCAAGCTTCCACTCGGCACCGTCCTGATCGATTCTCCTGCGTTTCTCTCCATGATGACCGAAATCAATAGATCTGATGGTCTTGCCGACGTCAAAGTCGATCAATCCGACTCGGCGGCAATCCTGTACTCGTCAGGAACGACAGGACGAGTGAAAGGCGTGTTACTGAGTCATCGAAACCTTATTGTGGTGAACTCTAGTCCTAATCCTCTTCTATCTGAGGTCAACAACGGAGAGACGGAGCCACGTCGGGTGTCTCTGTGTCTGTTGCCTCTGTTCCATGTTTTCGGATTCGTCATGTTGCTTCGAGCGATTTCACGAGGAGAAACATTGGTTCTGATGCAAAAATTCGACTTCGTGGAGATGCTATCAGCAGTAGAGAAGTTTAGAGTTACGTACATCCCAGTTTCTCCGCCGCTAGTGGTGGCGATGGCCAAGTCGGAACTGGTGGCGAAATACGATCTCAGTTCTCTTCAAGTTTTGGGGTGCGGCGGCGCTCCTCTTGGGAAAGAAGTCATCGAGAAATTCCATCAGAAATTGCCCAAAGTTGAAATTATCCAGGTTCGTAAGAAATACGTTCACTGTTTTTGCTCAATGATTTGTGTACAATAGACCTCGTTTCTTCTTCCCTTCATATGAATCTGATGAACTCTCCGTCCTACTTGTTGCCAAATCCGAATCTGTTCCTTCACTGACTTTTTTTTTTTTTTAAGTTTGTTTGGATTGAAATGTTAAATTAAAACGGAAGTGATTTTTACCATAAATTAAAAATTAAGAACTAATCTCAAGGTTAATCGATGAACTGAAATAGAATTTGAAAAACACAATCAAAATGATAGTAGTCCATATTCTTTGAAACTCAAACATACAAGTGTGTATTGTATTTTATGAACACTGTTGTTATAGACTTTACTAAACAAAAAATAGATTTGTTAAAAGTATTGATAATAAGAATGAATAGCTAAAGTTGACTTAAATCTCATTAATGTTCTTGTGGGGAGTGATGAAAGTTGTTCCATTTATTGTACCCTGTTTATTTATACCCATAATTAATTTTTCTTCCTGTTCATAGCATTACACGTTTCTCAATCCTGAGGGCCGCATTGTTTTGTTTTGTTGACTAGGGATATGGGTTGACAGAGAGTACAGCGGGGGCAGCAAGAAGTATGGGGCCCGAGGAAAGCAGCAACACGAAGTCAGTAGGCCGCCTATCTGAAAATATGGAAGCCATGATTGTGGATCCTGATTCTGGAGAGGCCTTGCCTCCTGGCCATAGAGGAGAGCTTTGGCTGCGAGGTCCAGGAATCATGAAAGGTTAGCAATCTTCTGTTCATCGTCATCAAACGTCCTCAAACACACGATCTTCCGCATTAATATGATATTGTCCAAGCATAAGAGCTTTACTTTTGACTTTTCCACAAGTCTCGTACTAATGGAGATATTGTTTTTTAATCATCCACCCGTGATCTTTCTCTTAATTAGCTTATATGGGACGACTCCCCTTAATAATTTTCAACAATCGTCCCCTCGAACAAAGTATACCATAGAGCTTCCCCTGAGTCCTCTTTCTTTAGAGCTCTTAAACAGTCTCCTCTTAATCGAGTCTCAACTCCTTCTCTGAAGCCCTCAGACAAAATACACCATTTATTCGACACTTGAGTCACCTTGGACTATACCTTCGAATCTCACAACTTTTTTTGTTCAACACTTAGAATTCTATTGACATGACTAAGCTAAGAGCAAGACTTTGATACCATGTTAGGAACCACGACCTTATATTGTCCACTTTAGGTATAAGCTCTCGTAGTTTTGTTTTTTTAGTTTCTCCAAAAGGTCTCGAGATAGTGTTCTTTACTTATATACTCATGATTTTCCCCATAATTAGTCGATGTGGGACTATTCCTCTTAATAATTTTCAACAAGAAACGGAGTACGTGGACTCCAATTTAATATGAGTTAACAGGAGAAAATGGCAGTGCGTACATTTAAACATGGGAGTCCCATTTGAATTCCATGTTGAGTTTTTGTTGATTCCATCATGAATCATTGAGCATGCATCATTGGCTTCTAACTCCTCAAATTCACAAGGACCATGATTGGGAATTGTTTGATCGTCTTTCTTCACCGTGCTATATGATGTTACTTATATACTAGACTCGATGCTTGTTCTATCTATGAACTGGAGTATAACTCAATCTATATTTTCAACTCATTGTTGAACTAAAAATAATAATATCCCGCCTCTATTTGCCTTGTGACATGAAGGTGATTTGTAAGGAATGCATTACACCCGAATATTGTTTGATAAACAGATCACCAAATATTGTTTGATTCAGGTTATGTAGGAGATGCCAAGGCAACTACCGAAACCCTGCATCCAGATGGATGGCTAAAGACTGGTGATCTTTGCTATTTTGATTCCGATGGATTCCTCTACATTGTTGACAGATTAAAAGAATTGATCAAGTACAAGGCTTATCAGGTGACTTCTTGTAATTTTTGACCGAATACGATTACAAATGTCAGTTGTCGTTATCGCGTTGTTGTCTTAGGTCCTATTTGAAATGACTTTCGAATCAAACCCACTGGTACGGGCTCCTTAGTAATTAAAAATCTTTTGCGTTTTGAACAAATTCCAGGTCCCACCTGCAGAATTGGAACATTTGCTTCAATCCAACCCTGAAATCATTGACGCTGCTGTGATACCGTATGCATCTGTCTGTTCTTAAACTTGTCAATAATGAGCTCTCTGCTACCTAATTTTGCTGATGAACTTGATTACTTCTGTTGAGCAGCTATCCCGATGAAGATGCTGGAGAGATTCCTATGGCTTACGTTGTTAGAAAGCCTGGAAGCAATATCACTGAGGCTCAAGTTATTGATTTCATTGCTAAACAGGCAAGCTTGAGATTGTCTCCTATTTCTCATGTCTTCTTCTTGCATTCAAGCTCACCATTTTCATCATCTTGCAGGTTGCACCGTACAAGAAAATTCGACGAGTTTCGTTTATCGACGCGATCCCGAAATCGCCTGCAGGGAAGATTCTGAGGAGGGAGCTGACCCAACATGCTCGCTCTCATGGTTCTAATAAGCTCTAA

mRNA sequence

ATCTGTTTTGGAATTTCCTATTGGTTGATTGTGCAATTTATTATTATTTTAATCTTGTAAAGTTGTTTTTGTTTTTTTAATCGGTTTGAATATTCAATCCAAAACATTCTCAACTAATTTGAATAAAATAAGTTGGATCAGCGTCCGCGTGCTCGATCGCGCATCACTTTCTTCGAGTCCACTTCCATGGCAGATCGGAATCCCAGCTTAAACGCAGCCCATTTCGTTGATCATCGAAGCGGTTTCTGTCCTCAGACGAAGATTTTCCATAGCCTCCGACCACCTCTCTTAATTCCGCCATTATCTCAGCCTCTCTCCGTTACCGAACACGCCCTTTCTCTCCTTCAATCGTCCCCTCCTCCTTCAAATGCCGCCGTTCTCGTCGACTCCAACTCTGGTGTGCATCTCTCCTATGCCATTTTCCTCCGTCAAATACGAACACTCGCCTCTAATCTTAAAGCGTTAACCTCACTCTCCAATGGTCAAGTGGCTTTTATTCTCGCACCGACTTCTTTGGAAGTCCCTGTGTTGTATTTTGCTCTGTTATCTCTCGGTGTTGTTATCTCTCCGGCTAATCCGATTGGTTCCGATTCGGAGATTTCTCATCAGATTCGACTCAGTGAACCGGTCATTGCCTTCGCTACATCCTCGACGGCGTCCAAGCTCCCCAAGCTTCCACTCGGCACCGTCCTGATCGATTCTCCTGCGTTTCTCTCCATGATGACCGAAATCAATAGATCTGATGGTCTTGCCGACGTCAAAGTCGATCAATCCGACTCGGCGGCAATCCTGTACTCGTCAGGAACGACAGGACGAGTGAAAGGCGTGTTACTGAGTCATCGAAACCTTATTGTGGTGAACTCTAGTCCTAATCCTCTTCTATCTGAGGTCAACAACGGAGAGACGGAGCCACGTCGGGTGTCTCTGTGTCTGTTGCCTCTGTTCCATGTTTTCGGATTCGTCATGTTGCTTCGAGCGATTTCACGAGGAGAAACATTGGTTCTGATGCAAAAATTCGACTTCGTGGAGATGCTATCAGCAGTAGAGAAGTTTAGAGTTACGTACATCCCAGTTTCTCCGCCGCTAGTGGTGGCGATGGCCAAGTCGGAACTGGTGGCGAAATACGATCTCAGTTCTCTTCAAGTTTTGGGGTGCGGCGGCGCTCCTCTTGGGAAAGAAGTCATCGAGAAATTCCATCAGAAATTGCCCAAAGTTGAAATTATCCAGGGATATGGGTTGACAGAGAGTACAGCGGGGGCAGCAAGAAGTATGGGGCCCGAGGAAAGCAGCAACACGAAGTCAGTAGGCCGCCTATCTGAAAATATGGAAGCCATGATTGTGGATCCTGATTCTGGAGAGGCCTTGCCTCCTGGCCATAGAGGAGAGCTTTGGCTGCGAGGTCCAGGAATCATGAAAGGTTATGTAGGAGATGCCAAGGCAACTACCGAAACCCTGCATCCAGATGGATGGCTAAAGACTGGTGATCTTTGCTATTTTGATTCCGATGGATTCCTCTACATTGTTGACAGATTAAAAGAATTGATCAAGTACAAGGCTTATCAGGTCCCACCTGCAGAATTGGAACATTTGCTTCAATCCAACCCTGAAATCATTGACGCTGCTGTGATACCCTATCCCGATGAAGATGCTGGAGAGATTCCTATGGCTTACGTTGTTAGAAAGCCTGGAAGCAATATCACTGAGGCTCAAGTTATTGATTTCATTGCTAAACAGGTTGCACCGTACAAGAAAATTCGACGAGTTTCGTTTATCGACGCGATCCCGAAATCGCCTGCAGGGAAGATTCTGAGGAGGGAGCTGACCCAACATGCTCGCTCTCATGGTTCTAATAAGCTCTAA

Coding sequence (CDS)

ATGGCAGATCGGAATCCCAGCTTAAACGCAGCCCATTTCGTTGATCATCGAAGCGGTTTCTGTCCTCAGACGAAGATTTTCCATAGCCTCCGACCACCTCTCTTAATTCCGCCATTATCTCAGCCTCTCTCCGTTACCGAACACGCCCTTTCTCTCCTTCAATCGTCCCCTCCTCCTTCAAATGCCGCCGTTCTCGTCGACTCCAACTCTGGTGTGCATCTCTCCTATGCCATTTTCCTCCGTCAAATACGAACACTCGCCTCTAATCTTAAAGCGTTAACCTCACTCTCCAATGGTCAAGTGGCTTTTATTCTCGCACCGACTTCTTTGGAAGTCCCTGTGTTGTATTTTGCTCTGTTATCTCTCGGTGTTGTTATCTCTCCGGCTAATCCGATTGGTTCCGATTCGGAGATTTCTCATCAGATTCGACTCAGTGAACCGGTCATTGCCTTCGCTACATCCTCGACGGCGTCCAAGCTCCCCAAGCTTCCACTCGGCACCGTCCTGATCGATTCTCCTGCGTTTCTCTCCATGATGACCGAAATCAATAGATCTGATGGTCTTGCCGACGTCAAAGTCGATCAATCCGACTCGGCGGCAATCCTGTACTCGTCAGGAACGACAGGACGAGTGAAAGGCGTGTTACTGAGTCATCGAAACCTTATTGTGGTGAACTCTAGTCCTAATCCTCTTCTATCTGAGGTCAACAACGGAGAGACGGAGCCACGTCGGGTGTCTCTGTGTCTGTTGCCTCTGTTCCATGTTTTCGGATTCGTCATGTTGCTTCGAGCGATTTCACGAGGAGAAACATTGGTTCTGATGCAAAAATTCGACTTCGTGGAGATGCTATCAGCAGTAGAGAAGTTTAGAGTTACGTACATCCCAGTTTCTCCGCCGCTAGTGGTGGCGATGGCCAAGTCGGAACTGGTGGCGAAATACGATCTCAGTTCTCTTCAAGTTTTGGGGTGCGGCGGCGCTCCTCTTGGGAAAGAAGTCATCGAGAAATTCCATCAGAAATTGCCCAAAGTTGAAATTATCCAGGGATATGGGTTGACAGAGAGTACAGCGGGGGCAGCAAGAAGTATGGGGCCCGAGGAAAGCAGCAACACGAAGTCAGTAGGCCGCCTATCTGAAAATATGGAAGCCATGATTGTGGATCCTGATTCTGGAGAGGCCTTGCCTCCTGGCCATAGAGGAGAGCTTTGGCTGCGAGGTCCAGGAATCATGAAAGGTTATGTAGGAGATGCCAAGGCAACTACCGAAACCCTGCATCCAGATGGATGGCTAAAGACTGGTGATCTTTGCTATTTTGATTCCGATGGATTCCTCTACATTGTTGACAGATTAAAAGAATTGATCAAGTACAAGGCTTATCAGGTCCCACCTGCAGAATTGGAACATTTGCTTCAATCCAACCCTGAAATCATTGACGCTGCTGTGATACCCTATCCCGATGAAGATGCTGGAGAGATTCCTATGGCTTACGTTGTTAGAAAGCCTGGAAGCAATATCACTGAGGCTCAAGTTATTGATTTCATTGCTAAACAGGTTGCACCGTACAAGAAAATTCGACGAGTTTCGTTTATCGACGCGATCCCGAAATCGCCTGCAGGGAAGATTCTGAGGAGGGAGCTGACCCAACATGCTCGCTCTCATGGTTCTAATAAGCTCTAA

Protein sequence

MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMTEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGETEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPLVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKILRRELTQHARSHGSNKL
BLAST of Carg07589 vs. NCBI nr
Match: XP_022923628.1 (4-coumarate--CoA ligase-like 9 [Cucurbita moschata])

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 554/557 (99.46%), Postives = 556/557 (99.82%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180
           SLGVVISPANPIGSDSEISHQIRLS+PVIAFATSSTASKLPKL LGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSKPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPR+VSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of Carg07589 vs. NCBI nr
Match: XP_023519270.1 (4-coumarate--CoA ligase-like 9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1067.0 bits (2758), Expect = 2.2e-308
Identity = 549/557 (98.56%), Postives = 554/557 (99.46%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVD RSGFCPQTKIF SLRPPL+IPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDIRSGFCPQTKIFRSLRPPLIIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180
           SLGVVISPANPIGSDSEISHQIRLS+PVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT
Sbjct: 121 SLGVVISPANPIGSDSEISHQIRLSQPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDG+ADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGIADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPR+VSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKF VTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFGVTYIPVSPPL 300

Query: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVA+AKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of Carg07589 vs. NCBI nr
Match: XP_023000795.1 (4-coumarate--CoA ligase-like 9 [Cucurbita maxima])

HSP 1 Score: 1063.1 bits (2748), Expect = 3.1e-307
Identity = 548/557 (98.38%), Postives = 552/557 (99.10%), Query Frame = 0

Query: 1   MADRNPSLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60
           MADRNPSLNAAHFVD RSGFCPQTKIF SLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS
Sbjct: 1   MADRNPSLNAAHFVDLRSGFCPQTKIFRSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPS 60

Query: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120
           NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL
Sbjct: 61  NAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALL 120

Query: 121 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMT 180
           SLGV+ISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKL LGTVLIDSPAFLSMMT
Sbjct: 121 SLGVIISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLQLGTVLIDSPAFLSMMT 180

Query: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240
           EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET
Sbjct: 181 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 240

Query: 241 EPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300
           EPR+VSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL
Sbjct: 241 EPRQVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPL 300

Query: 301 VVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360
           VVA+AKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR
Sbjct: 301 VVALAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 360

Query: 361 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 420
           SMGPEESSNTKSVGRLSENMEAMIVDP SGEALPPGHRGELWLRGPGIMKGYVGDA ATT
Sbjct: 361 SMGPEESSNTKSVGRLSENMEAMIVDPASGEALPPGHRGELWLRGPGIMKGYVGDADATT 420

Query: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480
           ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV
Sbjct: 421 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 480

Query: 481 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540
           IPYPDEDAGEIPMAYVVRKPGSN+TEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI
Sbjct: 481 IPYPDEDAGEIPMAYVVRKPGSNVTEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 540

Query: 541 LRRELTQHARSHGSNKL 558
           LRRELTQHARSHGSNKL
Sbjct: 541 LRRELTQHARSHGSNKL 557

BLAST of Carg07589 vs. NCBI nr
Match: XP_016899600.1 (PREDICTED: 4-coumarate--CoA ligase-like 9 [Cucumis melo])

HSP 1 Score: 912.5 bits (2357), Expect = 6.9e-262
Identity = 469/558 (84.05%), Postives = 505/558 (90.50%), Query Frame = 0

Query: 1   MADRNPSLNAA-HFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
           MA RN + NAA H +D RSGFCP TKIFHSLRPPL +PPLSQPLSVT+HA SLLQSS PP
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 60

Query: 61  SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
            N+ +L+DSNSG+HLSYAIFLRQIR LASNLK+LTSL NG VAFILAPTSL++PVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMM 180
           LSLGVV+SPANP  S SEISHQI+LS+PVIAFATSSTASKLP L  GTV+IDSP FLSM+
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 181 TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
           TE N SDGL D+K+DQSDSAAILYSSGTTGRVKGVLLSHRNLI VNS P    SE++ GE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 241 TEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
            +P  V+LCLLPLFHVFGFVML+RAISRGETLVLM++F+F  ML AVEKFRV YIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 301 LVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
           LVVAMAKS+LVAKYDLSSLQ+LGCGGAPLGKEVI+KFHQKLP VEI QGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 361 RSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKAT 420
           R+M PEE SNTKSVGRLS +MEA IVDP SGEAL P H+GELWLRGP IMKGYVGD KAT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
           TETL PDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 481 VIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540
           VIPYPDE+AGEIPMAYVVRKPGSNI+EAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540

Query: 541 ILRRELTQHARSHGSNKL 558
           ILRREL +HA SHGS+KL
Sbjct: 541 ILRRELAKHALSHGSSKL 558

BLAST of Carg07589 vs. NCBI nr
Match: ADN33954.1 (4-coumarate-CoA ligase [Cucumis melo subsp. melo])

HSP 1 Score: 904.0 bits (2335), Expect = 2.4e-259
Identity = 469/569 (82.43%), Postives = 505/569 (88.75%), Query Frame = 0

Query: 1    MADRNPSLNAA-HFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
            MA RN + NAA H +D RSGFCP TKIFHSLRPPL +PPLSQPLSVT+HA SLLQSS PP
Sbjct: 487  MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 546

Query: 61   SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
             N+ +L+DSNSG+HLSYAIFLRQIR LASNLK+LTSL NG VAFILAPTSL++PVLYFAL
Sbjct: 547  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 606

Query: 121  LSLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMM 180
            LSLGVV+SPANP  S SEISHQI+LS+PVIAFATSSTASKLP L  GTV+IDSP FLSM+
Sbjct: 607  LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 666

Query: 181  TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
            TE N SDGL D+K+DQSDSAAILYSSGTTGRVKGVLLSHRNLI VNS P    SE++ GE
Sbjct: 667  TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 726

Query: 241  TEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
             +P  V+LCLLPLFHVFGFVML+RAISRGETLVLM++F+F  ML AVEKFRV YIPVSPP
Sbjct: 727  MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 786

Query: 301  LVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
            LVVAMAKS+LVAKYDLSSLQ+LGCGGAPLGKEVI+KFHQKLP VEI QGYGLTESTAGAA
Sbjct: 787  LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 846

Query: 361  RSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKAT 420
            R+M PEE SNTKSVGRLS +MEA IVDP SGEAL P H+GELWLRGP IMKGYVGD KAT
Sbjct: 847  RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 906

Query: 421  TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
            TETL PDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA
Sbjct: 907  TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 966

Query: 481  VIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQ-----------VAPYKKIRRVSF 540
            VIPYPDE+AGEIPMAYVVRKPGSNI+EAQVIDFIAKQ           VAPYKKIRRVSF
Sbjct: 967  VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 1026

Query: 541  IDAIPKSPAGKILRRELTQHARSHGSNKL 558
            IDAIPKSPAGKILRREL +HA SHGS+KL
Sbjct: 1027 IDAIPKSPAGKILRRELAKHALSHGSSKL 1055

BLAST of Carg07589 vs. TAIR10
Match: AT5G63380.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 669.5 bits (1726), Expect = 1.8e-192
Identity = 356/565 (63.01%), Postives = 436/565 (77.17%), Query Frame = 0

Query: 9   NAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNA------ 68
           N +  +D  SGF  +T I+HSLRP L +PP+ QPLS  E ALSLL  S PP+ A      
Sbjct: 6   NDSRLIDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEA 65

Query: 69  -AVLVDSNSGVHLSYAIFLRQIRTLASNLK-ALTSLSNGQVAFILAPTSLEVPVLYFALL 128
              LV+S+SG +L+Y   LR++R+LA +L+    SL++  VAFIL+P+SL++PVLY AL+
Sbjct: 66  LTYLVNSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALM 125

Query: 129 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKL--PKLPLGTVLIDSPAFLSM 188
           S+GVV+SPANPIGS+SE+SHQ+ +SEPVIAFATS T  KL    LPLGTVL+DS  FLS 
Sbjct: 126 SIGVVVSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSW 185

Query: 189 MTEINRSDGLA----DVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVN--SSPNPLL 248
              +NRSD  +     V+V+QSD AAIL+SSGTTGRVKGVLL+HRNLI     S    L 
Sbjct: 186 ---LNRSDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQ 245

Query: 249 SEVNNGETEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVT 308
             VN       RV L  LPLFHVFGF+M++RAIS GETLVL+ +F+   M  AVEK++VT
Sbjct: 246 DPVNYD-----RVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVT 305

Query: 309 YIPVSPPLVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLT 368
            +PVSPPL+VA+ KSEL  KYDL SL+ LGCGGAPLGK++ E+F QK P V+I+QGYGLT
Sbjct: 306 GMPVSPPLIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLT 365

Query: 369 ESTAGAARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGY 428
           ES+  AA + GPEE     SVGR+SENMEA IVDP +GE+LPPG  GELWLRGP IMKGY
Sbjct: 366 ESSGPAASTFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGY 425

Query: 429 VGDAKATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN 488
           VG+ KA+ ET+  +GWLKTGDLCYFDS+ FLYIVDRLKELIKYKAYQVPP ELE +L SN
Sbjct: 426 VGNEKASAETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSN 485

Query: 489 PEIIDAAVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAI 548
           P++IDAAV+P+PDEDAGEIPMA++VRKPGSN+ EAQ+IDF+AKQV PYKK+RRV+FI+AI
Sbjct: 486 PDVIDAAVVPFPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVRRVAFINAI 545

Query: 549 PKSPAGKILRRELTQHARSHGSNKL 558
           PK+PAGKILRRELT+ A    ++KL
Sbjct: 546 PKNPAGKILRRELTKIAVDGNASKL 562

BLAST of Carg07589 vs. TAIR10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1)

HSP 1 Score: 454.9 bits (1169), Expect = 7.1e-128
Identity = 245/552 (44.38%), Postives = 362/552 (65.58%), Query Frame = 0

Query: 14  VDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGVH 73
           V+ RSGFC     F+S R P+ +PP +  L VT        SS         +D+++G +
Sbjct: 4   VNSRSGFCNSNSTFYSKRTPIPLPP-NPSLDVTTFI-----SSQAHRGRIAFIDASTGQN 63

Query: 74  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPIG 133
           L++    R + ++A  L  +  +  G V  +L+P S+  PV+  +++SLG +I+  NP+ 
Sbjct: 64  LTFTELWRAVESVADCLSEI-GIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 123

Query: 134 SDSEISHQIRLSEPVIAFAT-------SSTASKLPKLPLGTVLIDSPAFLSMMTEINR-- 193
           + +EI+ QI+ S PV+AF T       S+ A KLP + +    +DS   +  + E+ +  
Sbjct: 124 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE 183

Query: 194 -SDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGETEPR 253
            S      +VDQ D+A +LYSSGTTG  KGV+ SHRNLI +  +   +++   + + E R
Sbjct: 184 PSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQT---IVNRFGSDDGEQR 243

Query: 254 RVSLCLLPLFHVFGFVMLLRA-ISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPLVV 313
              +C +P+FH++G        ++ G T++++ KF+  EM+SA+ K++ T +P+ PP++V
Sbjct: 244 --FICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILV 303

Query: 314 AMAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 373
           AM     ++ AKYDLSS+  + CGGAPL KEV E F +K P V+I+QGYGLTEST   A 
Sbjct: 304 AMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGAS 363

Query: 374 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 433
           +   EES    + G+LS +ME  IVDP +G+ L P   GELWL+GP IMKGY  + +AT+
Sbjct: 364 TDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATS 423

Query: 434 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 493
            TL  +GWL+TGDLCY D DGF+++VDRLKELIKYK YQV PAELE LL ++PEI DAAV
Sbjct: 424 STLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAV 483

Query: 494 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 553
           IP+PD++ G+ PMAYVVRK GS+++E  +++F+AKQVAPYK+IR+V+F+ +IPK+P+GKI
Sbjct: 484 IPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKI 543

BLAST of Carg07589 vs. TAIR10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 453.8 bits (1166), Expect = 1.6e-127
Identity = 261/554 (47.11%), Postives = 351/554 (63.36%), Query Frame = 0

Query: 14  VDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGVH 73
           VD +SGFC  T IF+S R P+ +PP +Q L VT        +S P     V VD+ +G  
Sbjct: 21  VDRKSGFCESTSIFYSKREPMALPP-NQFLDVTSFI-----ASQPHRGKTVFVDAVTGRR 80

Query: 74  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPIG 133
           LS+      +  +A  L AL  +  G V  IL+P S+  P++  +++SLG +I+ ANPI 
Sbjct: 81  LSFPELWLGVERVAGCLYAL-GVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPIN 140

Query: 134 SDSEISHQIRLSEPVIAFATSSTASKL---PKLPLGTVLID-----SPAF---------L 193
           +  EIS QI  S PV+AF T    SKL       L  VL+D     S ++         L
Sbjct: 141 TSDEISKQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRL 200

Query: 194 SMMTEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVN 253
             M E   S+     +V+Q D+AA+LYSSGTTG  KGV+LSHRNLI         L +  
Sbjct: 201 ETMIETEPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA--------LVQAY 260

Query: 254 NGETEPRRVSLCLLPLFHVFGF-VMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIP 313
                  + ++C +P+ H+FGF       I+ G T+V++ KFD  ++LSAVE  R +Y+ 
Sbjct: 261 RARFGLEQRTICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLS 320

Query: 314 VSPPLVVAMAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTE 373
           + PP+VVAM    +E+ +KYDLSSL  +  GGAPL +EV EKF +  PKV+I+QGYGLTE
Sbjct: 321 LVPPIVVAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTE 380

Query: 374 STAGAARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYV 433
           STA AA     EE+    + G L+ N+E  IVDPD+G  L     GELW+R P +MKGY 
Sbjct: 381 STAIAASMFNKEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYF 440

Query: 434 GDAKATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNP 493
            + +AT  T+  +GWLKTGDLCY D DGF+++VDRLKELIK   YQV PAELE LL ++P
Sbjct: 441 KNKEATASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHP 500

Query: 494 EIIDAAVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIP 548
           EI DAAVIP PD  AG+ PMAY+VRK GSN++E++++ F+AKQV+PYKKIR+V+F+ +IP
Sbjct: 501 EIADAAVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIP 559

BLAST of Carg07589 vs. TAIR10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 442.6 bits (1137), Expect = 3.6e-124
Identity = 249/554 (44.95%), Postives = 349/554 (63.00%), Query Frame = 0

Query: 14  VDHRSGFCPQTKIFHSLRPPLLIPP-LSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGV 73
           VD RSGFC     F+S R PL +PP LS+ ++          SS P       +D+ +G 
Sbjct: 10  VDPRSGFCKSNSTFYSKRQPLSLPPNLSRDVTT-------FISSQPHRGKTAFIDAATGQ 69

Query: 74  HLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPI 133
            L+++   R +  +A  L     +  G V  IL+P S+ +PV+  +++SLG V + AN +
Sbjct: 70  CLTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTL 129

Query: 134 GSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAF------------LSMMT 193
            +  EIS QI  S P + F T   A KLP + +  VL D   +            LS M 
Sbjct: 130 NTSGEISKQIADSNPTLVFTTRQLAPKLP-VAISVVLTDDEVYQELTSAIRVVGILSEMV 189

Query: 194 EINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGET 253
           +   S      +V+Q D+A +LYSSGTTG  KGV+ SHRNL          ++   +   
Sbjct: 190 KKEPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNL-------TAHVARFISDNL 249

Query: 254 EPRRVSLCLLPLFHVFGFV-MLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 313
           +   + +C +P+FH +G +   +  ++ G T+V++++F   +M+ AVEK R T + ++PP
Sbjct: 250 KRDDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPP 309

Query: 314 LVVAMAKSE--LVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAG 373
           ++VAM      + AKYDLSSL+ + CGGAPL KEV E F +K P V+I+QGY LTES  G
Sbjct: 310 VLVAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGG 369

Query: 374 AARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAK 433
            A +   EES    + G L+ ++EA IVDP++G  +     GELWL+GP I KGY  + +
Sbjct: 370 GAFTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQE 429

Query: 434 ATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIID 493
           AT ET++ +GWLKTGDLCY D DGFL++VDRLKELIKYK YQVPPAELE LL ++P+I+D
Sbjct: 430 ATNETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILD 489

Query: 494 AAVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPA 552
           AAVIP+PD++AG+ PMAYVVRK  SN++E QVIDFI+KQVAPYKKIR+VSFI++IPK+ +
Sbjct: 490 AAVIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTAS 548

BLAST of Carg07589 vs. TAIR10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 423.3 bits (1087), Expect = 2.3e-118
Identity = 239/560 (42.68%), Postives = 347/560 (61.96%), Query Frame = 0

Query: 7   SLNAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLV 66
           S  ++  +D R+GFC     F+S R PL +P   + L +T        SS         +
Sbjct: 4   SQRSSSLIDPRNGFCTSNSTFYSKRKPLALPS-KESLDITTFI-----SSQTYRGKTAFI 63

Query: 67  DSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVI 126
           D+ +   +S++     +  +A  L     +  G V  +L+P ++ +P++  +++SLG V+
Sbjct: 64  DAATDHRISFSDLWMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAVL 123

Query: 127 SPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVL------IDSPAFLSM-- 186
           + ANP+ + SEI  QI  S P +AF T   A K+    +  VL      +  P  L +  
Sbjct: 124 TTANPLNTASEILRQIADSNPKLAFTTPELAPKIASSGISIVLERVEDTLRVPRGLKVVG 183

Query: 187 -MTEINRSDGLADV---KVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSE 246
            +TE+ + +        +V + D+A +LYSSGTTGR KGV  SH NLI        +   
Sbjct: 184 NLTEMMKKEPSGQAVRNQVHKDDTAMLLYSSGTTGRSKGVNSSHGNLIA------HVARY 243

Query: 247 VNNGETEPRRVSLCLLPLFHVFGFV-MLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTY 306
           +     +P++  +C +PLFH FG +  +L  ++ G T+V++ +FD  EM++AVEK+R T 
Sbjct: 244 IAEPFEQPQQTFICTVPLFHTFGLLNFVLATLALGTTVVILPRFDLGEMMAAVEKYRATT 303

Query: 307 IPVSPPLVVAMAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGL 366
           + + PP++V M     +++ KYD+S L+ + CGGAPL KEV + F +K P V++ QGY L
Sbjct: 304 LILVPPVLVTMINKADQIMKKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQGYAL 363

Query: 367 TESTAGAARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKG 426
           TES    A     EES    +VG LS  +EA IVDP++G+ +     GELWL+GP I KG
Sbjct: 364 TESNGAGASIESVEESRRYGAVGLLSCGVEARIVDPNTGQVMGLNQTGELWLKGPSIAKG 423

Query: 427 YVGDAKATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQS 486
           Y  + +   E +  +GWLKTGDLCY D+DGFL+IVDRLKELIKYK YQVPPAELE LL +
Sbjct: 424 YFRNEE---EIITSEGWLKTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEALLLN 483

Query: 487 NPEIIDAAVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDA 546
           +P+I+DAAVIP+PD++AG+ PMAYV RKP SN+ E +VIDFI+KQVAPYKKIR+V+FID+
Sbjct: 484 HPDILDAAVIPFPDKEAGQFPMAYVARKPESNLCEKKVIDFISKQVAPYKKIRKVAFIDS 543

Query: 547 IPKSPAGKILRRELTQHARS 552
           IPK+P+GK LR++L + A S
Sbjct: 544 IPKTPSGKTLRKDLIKFAIS 548

BLAST of Carg07589 vs. Swiss-Prot
Match: sp|Q84P23|4CLL9_ARATH (4-coumarate--CoA ligase-like 9 OS=Arabidopsis thaliana OX=3702 GN=4CLL9 PE=1 SV=2)

HSP 1 Score: 669.5 bits (1726), Expect = 3.3e-191
Identity = 356/565 (63.01%), Postives = 436/565 (77.17%), Query Frame = 0

Query: 9   NAAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNA------ 68
           N +  +D  SGF  +T I+HSLRP L +PP+ QPLS  E ALSLL  S PP+ A      
Sbjct: 6   NDSRLIDRSSGFDQRTGIYHSLRPSLSLPPIDQPLSAAEFALSLLLKSSPPATAGKNIEA 65

Query: 69  -AVLVDSNSGVHLSYAIFLRQIRTLASNLK-ALTSLSNGQVAFILAPTSLEVPVLYFALL 128
              LV+S+SG +L+Y   LR++R+LA +L+    SL++  VAFIL+P+SL++PVLY AL+
Sbjct: 66  LTYLVNSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALM 125

Query: 129 SLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKL--PKLPLGTVLIDSPAFLSM 188
           S+GVV+SPANPIGS+SE+SHQ+ +SEPVIAFATS T  KL    LPLGTVL+DS  FLS 
Sbjct: 126 SIGVVVSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSW 185

Query: 189 MTEINRSDGLA----DVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVN--SSPNPLL 248
              +NRSD  +     V+V+QSD AAIL+SSGTTGRVKGVLL+HRNLI     S    L 
Sbjct: 186 ---LNRSDSSSVNPFQVQVNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQ 245

Query: 249 SEVNNGETEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVT 308
             VN       RV L  LPLFHVFGF+M++RAIS GETLVL+ +F+   M  AVEK++VT
Sbjct: 246 DPVNYD-----RVGLFSLPLFHVFGFMMMIRAISLGETLVLLGRFELEAMFKAVEKYKVT 305

Query: 309 YIPVSPPLVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLT 368
            +PVSPPL+VA+ KSEL  KYDL SL+ LGCGGAPLGK++ E+F QK P V+I+QGYGLT
Sbjct: 306 GMPVSPPLIVALVKSELTKKYDLRSLRSLGCGGAPLGKDIAERFKQKFPDVDIVQGYGLT 365

Query: 369 ESTAGAARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGY 428
           ES+  AA + GPEE     SVGR+SENMEA IVDP +GE+LPPG  GELWLRGP IMKGY
Sbjct: 366 ESSGPAASTFGPEEMVKYGSVGRISENMEAKIVDPSTGESLPPGKTGELWLRGPVIMKGY 425

Query: 429 VGDAKATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSN 488
           VG+ KA+ ET+  +GWLKTGDLCYFDS+ FLYIVDRLKELIKYKAYQVPP ELE +L SN
Sbjct: 426 VGNEKASAETVDKEGWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSN 485

Query: 489 PEIIDAAVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAI 548
           P++IDAAV+P+PDEDAGEIPMA++VRKPGSN+ EAQ+IDF+AKQV PYKK+RRV+FI+AI
Sbjct: 486 PDVIDAAVVPFPDEDAGEIPMAFIVRKPGSNLNEAQIIDFVAKQVTPYKKVRRVAFINAI 545

Query: 549 PKSPAGKILRRELTQHARSHGSNKL 558
           PK+PAGKILRRELT+ A    ++KL
Sbjct: 546 PKNPAGKILRRELTKIAVDGNASKL 562

BLAST of Carg07589 vs. Swiss-Prot
Match: sp|Q7F1X5|4CLL5_ORYSJ (4-coumarate--CoA ligase-like 5 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL5 PE=2 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 7.0e-157
Identity = 299/551 (54.26%), Postives = 387/551 (70.24%), Query Frame = 0

Query: 14  VDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGVH 73
           +D RSGFC  T+IFHS R P  +PP S P++   +A SLL SS  P   A LVD+ +G+ 
Sbjct: 15  IDSRSGFCAATRIFHSTRAPGDLPPESLPMTAAAYAFSLLSSSTLPGRPA-LVDAATGIA 74

Query: 74  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPIG 133
           +SY  FL  +R+LA  L     L  G VA ++AP+ LEVPVL FAL+S+G V+SPANP+ 
Sbjct: 75  ISYPSFLAAVRSLAGGLWCSLGLRPGDVALVVAPSRLEVPVLDFALMSIGAVVSPANPVS 134

Query: 134 SDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSM-MTEINRSDGLADVK 193
           +  E +HQ+ LS PV+AFA    A+KLP+  +  V+I S  +  +  ++  R+   A V 
Sbjct: 135 TPEEYAHQVALSRPVVAFAAPEVAAKLPE-HVRCVVIGSDEYGRLAASDGRRAAAPAAVA 194

Query: 194 VDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGETE------PRRVS 253
           V QSD+AA+LYSSGTTGRVK V ++HRNLI + S       +V     E      P  V+
Sbjct: 195 VKQSDTAAVLYSSGTTGRVKAVAITHRNLIALMSLHADNREKVAREAAEAGEEPPPPAVT 254

Query: 254 LCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPLVVAMAK 313
           L  +PLFHVFGF+M+LR++S GET VLM++FDF+  L A+E++RVT +P +PP++VAM K
Sbjct: 255 LLPIPLFHVFGFMMVLRSVSMGETSVLMERFDFIAALRAIERYRVTLLPAAPPVLVAMVK 314

Query: 314 SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAARSMGPEE 373
            E   + DLSSL V+G GGAPLG+EV E+F    P VE++QGYGLTES+   A ++GPEE
Sbjct: 315 YEEARRRDLSSLLVIGIGGAPLGREVAEQFASVFPNVELVQGYGLTESSGAVAATVGPEE 374

Query: 374 SSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATTETLHPD 433
           S    SVG+L  +++A IVDP +                     GYVGD +AT  T+  +
Sbjct: 375 SKAYGSVGKLGSHLQAKIVDPST---------------------GYVGDDEATAATVDSE 434

Query: 434 GWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAVIPYPDE 493
           GWLKTGDLCYF+ DGFLYIVDRLKELIKYK YQVPPAELEH+LQS+P I DAAVIPYPDE
Sbjct: 435 GWLKTGDLCYFNEDGFLYIVDRLKELIKYKGYQVPPAELEHILQSHPGIADAAVIPYPDE 494

Query: 494 DAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKILRRELT 553
           +AGE+PMA++VR+PGSNIT+ QV+D++AKQVAPYKK+RRV+F+ AIPKSPAGKILRREL 
Sbjct: 495 EAGELPMAFIVRQPGSNITKEQVMDYVAKQVAPYKKVRRVAFVTAIPKSPAGKILRRELV 542

Query: 554 QHARSHGSNKL 558
           Q A S G++KL
Sbjct: 555 QQALSMGASKL 542

BLAST of Carg07589 vs. Swiss-Prot
Match: sp|Q8RU95|4CLL6_ORYSJ (4-coumarate--CoA ligase-like 6 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL6 PE=2 SV=2)

HSP 1 Score: 538.5 bits (1386), Expect = 8.8e-152
Identity = 291/558 (52.15%), Postives = 377/558 (67.56%), Query Frame = 0

Query: 17  RSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPP---PSNAAVLVDSNSGVH 76
           RSGFC  T+ FHSLR    +PP   PL+V  +A SLL S+PP         LVD+ +G+ 
Sbjct: 41  RSGFCAATRTFHSLRSVGPLPPEELPLTVAAYAFSLLSSAPPLVVAGRGPALVDAATGIA 100

Query: 77  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPIG 136
           +SY  F+ ++R LA  L     L  G VA +++P+ L+V VLYFAL+S+GVV+SPANP  
Sbjct: 101 VSYPAFVARVRFLAGGLWCSLGLRPGDVALVVSPSCLDVAVLYFALMSIGVVVSPANPAS 160

Query: 137 SDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMTEINRSDGLA---D 196
           +  E +HQ+RLS P IAF     A++LP+  +  V+I S  F   +   + + G A    
Sbjct: 161 TADEYAHQVRLSRPAIAFVAPEVAARLPR-HVSRVVIGSEVF-DRLASASAAGGWAAPPA 220

Query: 197 VKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEV-NNGETEPRRVSLCL 256
           V + Q  +AA+LYSSGTTGRVK V ++HRNLI   S+ N +   V     T+        
Sbjct: 221 VAMKQPSTAALLYSSGTTGRVKAVAITHRNLIAQISAYNAIRETVAREAATDAGXXXXXX 280

Query: 257 -------------LPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPV 316
                        LPLFHV GF +L R IS GET V+M++FD      AVE++RVT +  
Sbjct: 281 XXXXXXXXXXXXPLPLFHVMGFGLLTRTISSGETAVVMRRFDLAAAARAVERYRVTKLSA 340

Query: 317 SPPLVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTA 376
           +PP+VVA+ KS+   + DLSSL  +  GGAPLG+EV ++F    P V+I+Q YGLTEST 
Sbjct: 341 APPVVVALTKSDEARRRDLSSLVAIVVGGAPLGREVSQRFATVFPSVQIVQSYGLTESTG 400

Query: 377 GAARSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDA 436
             A   GPEES+   SVGRL+  ++A IVD  +GE L PG RGELW+RGP +MKGYVGD 
Sbjct: 401 PVATMAGPEESAAYGSVGRLAPRVQAKIVDTATGEVLGPGRRGELWIRGPVVMKGYVGDP 460

Query: 437 KATTETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEII 496
           +AT  T+ PDGWLKTGDLCYF+ DG+LY+VDRLKELIKYK YQVPPAELEH+LQS PEI 
Sbjct: 461 EATAATITPDGWLKTGDLCYFNEDGYLYVVDRLKELIKYKGYQVPPAELEHILQSRPEIA 520

Query: 497 DAAVIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSP 555
           DAAV+PYPDE+AG++PMA+VVR+PG+ +TE QV++ +AK VAPYKK+RRV+F++AIPKSP
Sbjct: 521 DAAVVPYPDEEAGQLPMAFVVRQPGAYLTEQQVMNCVAKHVAPYKKVRRVAFVNAIPKSP 580

BLAST of Carg07589 vs. Swiss-Prot
Match: sp|Q69RG7|4CLL7_ORYSJ (4-coumarate--CoA ligase-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL7 PE=2 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 2.6e-135
Identity = 268/548 (48.91%), Postives = 365/548 (66.61%), Query Frame = 0

Query: 17  RSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPP-------PSNAAVLVDSN 76
           R+G+C  TK F SLRPP+ +PP   PLS  E A SLL  S         P+N A LVD+ 
Sbjct: 16  RTGYCAATKSFRSLRPPVPLPPPDVPLSFPEFAFSLLPRSSSXXXXSLLPANPA-LVDAA 75

Query: 77  SGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPA 136
           +G  +S+  FL ++R LA  L++   L  G VAF+LAP  L+VPVLYFALLS+G V+SPA
Sbjct: 76  TGEAVSFQAFLSRVRALAGALRSRVGLRGGDVAFVLAPAGLDVPVLYFALLSIGAVVSPA 135

Query: 137 NPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMTEINRSDG-- 196
           NP  + +E+S  + LS   +AFA SSTA+KLP      VL+DSP F S++ +  ++ G  
Sbjct: 136 NPALTPAEVSRLVSLSGASVAFAVSSTATKLPAGLTTVVLLDSPHFRSLLMDCGQAQGQE 195

Query: 197 -LADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGETEPRRVS 256
            L  V V QS++AAI YSSGTTGRVK   L HR+ I + +  + L ++     T      
Sbjct: 196 PLPVVVVRQSETAAIQYSSGTTGRVKAAALPHRSFIAMVAGFHALRAKAREVRT------ 255

Query: 257 LCLLPLFHVFGFVMLLRAISRGETLVL----MQKFDFVEMLSAVEKFRVTYIPVSPPLVV 316
           L   P+FH  GF+ +L+ ++ G T V+    + +     ++ A E++ V  +  SPP+V+
Sbjct: 256 LLGAPMFHSMGFLFVLQGVALGATTVVVTDAVARAGIRGLVEAAERWAVMDMTASPPVVL 315

Query: 317 AMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAARSM 376
            M K     +  L +L+ + CGGAPL    IE+F ++ P V++  GYG TE+  G +R +
Sbjct: 316 GMTKQ----RCRLPALERITCGGAPLPAPAIERFRRRFPHVDLCMGYGSTEA-GGISRMI 375

Query: 377 GPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATTET 436
             EE ++  S GR++EN+E  IVD  +G+ LP G +GELW+RGP +M GYVGD +A   T
Sbjct: 376 SQEECNHIGSAGRVTENVEVKIVDHVTGKPLPAGQQGELWVRGPAVMTGYVGDNEANATT 435

Query: 437 LHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAVIP 496
            + +GWLKTGDLCY D DGFL++VDRLKELIKYKAYQVPPAELE +L S P+I+DAAV+P
Sbjct: 436 FNSEGWLKTGDLCYIDQDGFLFVVDRLKELIKYKAYQVPPAELELVLHSLPQIVDAAVMP 495

Query: 497 YPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKILR 551
           YP E+AG+IP+A VV++PGS +TEA+V+  +AKQVAPYKKIR+V F+D+IPKSP+GKILR
Sbjct: 496 YPHEEAGQIPVALVVKQPGSKLTEAEVMYNVAKQVAPYKKIRKVLFVDSIPKSPSGKILR 551

BLAST of Carg07589 vs. Swiss-Prot
Match: sp|Q84P21|4CLL5_ARATH (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 454.9 bits (1169), Expect = 1.3e-126
Identity = 245/552 (44.38%), Postives = 362/552 (65.58%), Query Frame = 0

Query: 14  VDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSNSGVH 73
           V+ RSGFC     F+S R P+ +PP +  L VT        SS         +D+++G +
Sbjct: 4   VNSRSGFCNSNSTFYSKRTPIPLPP-NPSLDVTTFI-----SSQAHRGRIAFIDASTGQN 63

Query: 74  LSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPANPIG 133
           L++    R + ++A  L  +  +  G V  +L+P S+  PV+  +++SLG +I+  NP+ 
Sbjct: 64  LTFTELWRAVESVADCLSEI-GIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 123

Query: 134 SDSEISHQIRLSEPVIAFAT-------SSTASKLPKLPLGTVLIDSPAFLSMMTEINR-- 193
           + +EI+ QI+ S PV+AF T       S+ A KLP + +    +DS   +  + E+ +  
Sbjct: 124 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE 183

Query: 194 -SDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGETEPR 253
            S      +VDQ D+A +LYSSGTTG  KGV+ SHRNLI +  +   +++   + + E R
Sbjct: 184 PSGNRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQT---IVNRFGSDDGEQR 243

Query: 254 RVSLCLLPLFHVFGFVMLLRA-ISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPLVV 313
              +C +P+FH++G        ++ G T++++ KF+  EM+SA+ K++ T +P+ PP++V
Sbjct: 244 --FICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILV 303

Query: 314 AMAK--SELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAAR 373
           AM     ++ AKYDLSS+  + CGGAPL KEV E F +K P V+I+QGYGLTEST   A 
Sbjct: 304 AMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGAS 363

Query: 374 SMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATT 433
           +   EES    + G+LS +ME  IVDP +G+ L P   GELWL+GP IMKGY  + +AT+
Sbjct: 364 TDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATS 423

Query: 434 ETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAV 493
            TL  +GWL+TGDLCY D DGF+++VDRLKELIKYK YQV PAELE LL ++PEI DAAV
Sbjct: 424 STLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAV 483

Query: 494 IPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKI 553
           IP+PD++ G+ PMAYVVRK GS+++E  +++F+AKQVAPYK+IR+V+F+ +IPK+P+GKI
Sbjct: 484 IPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKI 543

BLAST of Carg07589 vs. TrEMBL
Match: tr|A0A1S4DV63|A0A1S4DV63_CUCME (4-coumarate--CoA ligase-like 9 OS=Cucumis melo OX=3656 GN=LOC103489008 PE=4 SV=1)

HSP 1 Score: 912.5 bits (2357), Expect = 4.5e-262
Identity = 469/558 (84.05%), Postives = 505/558 (90.50%), Query Frame = 0

Query: 1   MADRNPSLNAA-HFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
           MA RN + NAA H +D RSGFCP TKIFHSLRPPL +PPLSQPLSVT+HA SLLQSS PP
Sbjct: 1   MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 60

Query: 61  SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
            N+ +L+DSNSG+HLSYAIFLRQIR LASNLK+LTSL NG VAFILAPTSL++PVLYFAL
Sbjct: 61  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMM 180
           LSLGVV+SPANP  S SEISHQI+LS+PVIAFATSSTASKLP L  GTV+IDSP FLSM+
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 180

Query: 181 TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
           TE N SDGL D+K+DQSDSAAILYSSGTTGRVKGVLLSHRNLI VNS P    SE++ GE
Sbjct: 181 TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 240

Query: 241 TEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
            +P  V+LCLLPLFHVFGFVML+RAISRGETLVLM++F+F  ML AVEKFRV YIPVSPP
Sbjct: 241 MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 300

Query: 301 LVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
           LVVAMAKS+LVAKYDLSSLQ+LGCGGAPLGKEVI+KFHQKLP VEI QGYGLTESTAGAA
Sbjct: 301 LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 360

Query: 361 RSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKAT 420
           R+M PEE SNTKSVGRLS +MEA IVDP SGEAL P H+GELWLRGP IMKGYVGD KAT
Sbjct: 361 RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
           TETL PDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 481 VIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540
           VIPYPDE+AGEIPMAYVVRKPGSNI+EAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540

Query: 541 ILRRELTQHARSHGSNKL 558
           ILRREL +HA SHGS+KL
Sbjct: 541 ILRRELAKHALSHGSSKL 558

BLAST of Carg07589 vs. TrEMBL
Match: tr|E5GBV5|E5GBV5_CUCME (4-coumarate-CoA ligase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 1.6e-259
Identity = 469/569 (82.43%), Postives = 505/569 (88.75%), Query Frame = 0

Query: 1    MADRNPSLNAA-HFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
            MA RN + NAA H +D RSGFCP TKIFHSLRPPL +PPLSQPLSVT+HA SLLQSS PP
Sbjct: 487  MAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQSSSPP 546

Query: 61   SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
             N+ +L+DSNSG+HLSYAIFLRQIR LASNLK+LTSL NG VAFILAPTSL++PVLYFAL
Sbjct: 547  PNSTLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIPVLYFAL 606

Query: 121  LSLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMM 180
            LSLGVV+SPANP  S SEISHQI+LS+PVIAFATSSTASKLP L  GTV+IDSP FLSM+
Sbjct: 607  LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSPHFLSML 666

Query: 181  TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
            TE N SDGL D+K+DQSDSAAILYSSGTTGRVKGVLLSHRNLI VNS P    SE++ GE
Sbjct: 667  TETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNSGPTAFQSEIHEGE 726

Query: 241  TEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
             +P  V+LCLLPLFHVFGFVML+RAISRGETLVLM++F+F  ML AVEKFRV YIPVSPP
Sbjct: 727  MKPHPVALCLLPLFHVFGFVMLVRAISRGETLVLMERFEFEGMLRAVEKFRVIYIPVSPP 786

Query: 301  LVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
            LVVAMAKS+LVAKYDLSSLQ+LGCGGAPLGKEVI+KFHQKLP VEI QGYGLTESTAGAA
Sbjct: 787  LVVAMAKSDLVAKYDLSSLQILGCGGAPLGKEVIDKFHQKLPSVEIAQGYGLTESTAGAA 846

Query: 361  RSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKAT 420
            R+M PEE SNTKSVGRLS +MEA IVDP SGEAL P H+GELWLRGP IMKGYVGD KAT
Sbjct: 847  RTMEPEEISNTKSVGRLSGSMEAKIVDPASGEALLPNHKGELWLRGPTIMKGYVGDDKAT 906

Query: 421  TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
            TETL PDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA
Sbjct: 907  TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 966

Query: 481  VIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQ-----------VAPYKKIRRVSF 540
            VIPYPDE+AGEIPMAYVVRKPGSNI+EAQVIDFIAKQ           VAPYKKIRRVSF
Sbjct: 967  VIPYPDEEAGEIPMAYVVRKPGSNISEAQVIDFIAKQAMLHLSRNFQIVAPYKKIRRVSF 1026

Query: 541  IDAIPKSPAGKILRRELTQHARSHGSNKL 558
            IDAIPKSPAGKILRREL +HA SHGS+KL
Sbjct: 1027 IDAIPKSPAGKILRRELAKHALSHGSSKL 1055

BLAST of Carg07589 vs. TrEMBL
Match: tr|A0A0A0LTS5|A0A0A0LTS5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G050280 PE=4 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 2.3e-258
Identity = 458/558 (82.08%), Postives = 501/558 (89.78%), Query Frame = 0

Query: 1   MADRNPSLNAA-HFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPP 60
           MADRN +   + H +D RSGFCPQTKIFHSLRPPL +PPLS PLSV +HA SLLQSSPPP
Sbjct: 1   MADRNSNFTTSPHSIDLRSGFCPQTKIFHSLRPPLSLPPLSHPLSVIQHAFSLLQSSPPP 60

Query: 61  SNAAVLVDSNSGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFAL 120
            N+ +L+D NSG+HLSY +FLRQIR LASNLK+LTSL NGQVAFILAPTSL++PVLYFAL
Sbjct: 61  PNSTLLIDFNSGLHLSYTVFLRQIRNLASNLKSLTSLCNGQVAFILAPTSLQIPVLYFAL 120

Query: 121 LSLGVVISPANPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMM 180
           LSLGVV+SPANP  S SEISHQI+LS+PVIAFATSSTASKLPKL  GTVLIDSP F SM+
Sbjct: 121 LSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPKLRFGTVLIDSPHFHSML 180

Query: 181 TEINRSDGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGE 240
           TE N SDG+ ++K+DQSDSAAILYSSGTTGRVKGVLLSHRNLI VN+ PN L  E+  GE
Sbjct: 181 TETNGSDGIPNIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAVNAGPNSLQLEIQEGE 240

Query: 241 TEPRRVSLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPP 300
            EP  V LC LPLFH+FGFVML+RA+SRG+TLVLM++F+F  ML AVEK+RV YIPVSPP
Sbjct: 241 MEPHPVVLCPLPLFHIFGFVMLVRAVSRGDTLVLMERFEFEGMLRAVEKYRVKYIPVSPP 300

Query: 301 LVVAMAKSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAA 360
           LVVAMAKSELV KYDLSSLQ+LGCGGAPLGKEVI+KFHQKLP VEI+QGYGLTESTAGA+
Sbjct: 301 LVVAMAKSELVGKYDLSSLQMLGCGGAPLGKEVIDKFHQKLPGVEIVQGYGLTESTAGAS 360

Query: 361 RSMGPEESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKAT 420
           R+M PEE SNTKSVGRLS NMEA IVDP SG+AL P H+GELWLRGP IMKGYVGD KAT
Sbjct: 361 RTMDPEEISNTKSVGRLSGNMEAKIVDPASGDALLPNHKGELWLRGPTIMKGYVGDDKAT 420

Query: 421 TETLHPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480
           TETL PDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA
Sbjct: 421 TETLDPDGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAA 480

Query: 481 VIPYPDEDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540
           VIPYPDE+AGEIPMAYVVRKPGSN++EAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK
Sbjct: 481 VIPYPDEEAGEIPMAYVVRKPGSNMSEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGK 540

Query: 541 ILRRELTQHARSHGSNKL 558
           ILRREL +HA SHGS+KL
Sbjct: 541 ILRRELAKHALSHGSSKL 558

BLAST of Carg07589 vs. TrEMBL
Match: tr|A0A1S4DUD8|A0A1S4DUD8_CUCME (4-coumarate--CoA ligase-like 9 isoform X2 OS=Cucumis melo OX=3656 GN=LOC107990308 PE=4 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 4.4e-241
Identity = 426/552 (77.17%), Postives = 480/552 (86.96%), Query Frame = 0

Query: 10  AAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSN 69
           A H VD RSGFC QTKI+ SLRPPL +PPLSQPL+V  HALS+L+SSPPP N A L+DS+
Sbjct: 2   ATHSVDSRSGFCSQTKIYKSLRPPLSLPPLSQPLTVAGHALSVLRSSPPPPNTAALIDSD 61

Query: 70  SGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPA 129
           SGV +SYA+FLRQIR L SNLKAL S SNGQVAFIL+PTSL++PVLYFALLSLGVV+SPA
Sbjct: 62  SGVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFALLSLGVVVSPA 121

Query: 130 NPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMTEINRS---- 189
           NPI S+SEI+HQ+ L +PVIAFA SSTASK+P+LPLGTVLIDSP FLS+M E NRS    
Sbjct: 122 NPISSESEIAHQVSLCKPVIAFAISSTASKIPRLPLGTVLIDSPEFLSLMNESNRSEGVN 181

Query: 190 DGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGETEPRRV 249
           DG+ D+K++Q+DSAAILYSSGTTGRVKGVLLSHRNLI   +    L     +GE EP  V
Sbjct: 182 DGIFDLKINQNDSAAILYSSGTTGRVKGVLLSHRNLITAITGVQVLDKTPVDGEIEPHPV 241

Query: 250 SLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPLVVAMA 309
           +L LLPLFHVFGF ML R+IS G TLVLM+KFDF +ML AVEK+RVTYIPVSPPLV+AMA
Sbjct: 242 ALSLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRVTYIPVSPPLVLAMA 301

Query: 310 KSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAARSMGPE 369
           KSEL  KYDLSSLQ+LGCGGAPLGKEVI+KFH K P VEIIQGYGLTES   A+R++GPE
Sbjct: 302 KSELAEKYDLSSLQILGCGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESAGAASRTVGPE 361

Query: 370 ESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATTETLHP 429
           E S   SVGRLSENMEA IVDP SGEALPPGH+GELW+RGPGIMKGYVGD +AT ETLHP
Sbjct: 362 ECSKASSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLHP 421

Query: 430 DGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAVIPYPD 489
           +GWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQS+PEI+DAAVIPYPD
Sbjct: 422 EGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYPD 481

Query: 490 EDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKILRREL 549
           E+AG+IP+AYVVR+PGSNI E QV+DFIAKQVAPYKKIRRV F++A+PKSPAGKILRREL
Sbjct: 482 EEAGQIPVAYVVRQPGSNINETQVMDFIAKQVAPYKKIRRVFFVNAVPKSPAGKILRREL 541

Query: 550 TQHARSHGSNKL 558
            QHA S GS++L
Sbjct: 542 VQHALSQGSSRL 553

BLAST of Carg07589 vs. TrEMBL
Match: tr|A0A0A0LWL5|A0A0A0LWL5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G050300 PE=4 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 4.9e-240
Identity = 424/552 (76.81%), Postives = 480/552 (86.96%), Query Frame = 0

Query: 10  AAHFVDHRSGFCPQTKIFHSLRPPLLIPPLSQPLSVTEHALSLLQSSPPPSNAAVLVDSN 69
           A H VD RSGFC QTKI++SLRPPL +PPLSQPL+V  HALS+L+SSPPP N   L+DS+
Sbjct: 2   ATHSVDSRSGFCSQTKIYNSLRPPLSLPPLSQPLTVVGHALSVLRSSPPPPNTPALIDSD 61

Query: 70  SGVHLSYAIFLRQIRTLASNLKALTSLSNGQVAFILAPTSLEVPVLYFALLSLGVVISPA 129
           S V +SYA+FLRQIR L SNLKAL S SNGQVAFIL+PTSL++PVLYF LLSLGVV+SPA
Sbjct: 62  SSVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFVLLSLGVVVSPA 121

Query: 130 NPIGSDSEISHQIRLSEPVIAFATSSTASKLPKLPLGTVLIDSPAFLSMMTEINRS---- 189
           NPIGS+SEI+HQ+ L  P IAFATSSTASK+P+LPLGTVLIDSP FLS+M E N S    
Sbjct: 122 NPIGSESEIAHQVSLCIPAIAFATSSTASKIPRLPLGTVLIDSPEFLSLMNESNPSDGVN 181

Query: 190 DGLADVKVDQSDSAAILYSSGTTGRVKGVLLSHRNLIVVNSSPNPLLSEVNNGETEPRRV 249
           DG+ D+K++Q+DSAAILYSSGTTGRVKGVL+SHRNLI   +    L   + +GE EP  V
Sbjct: 182 DGIFDLKINQNDSAAILYSSGTTGRVKGVLISHRNLITAITGLQVLDKTLVDGEIEPHPV 241

Query: 250 SLCLLPLFHVFGFVMLLRAISRGETLVLMQKFDFVEMLSAVEKFRVTYIPVSPPLVVAMA 309
           +LCLLPLFHVFGF ML R+IS G TLVLM+KFDF +ML AVEK+R+TYIPVSPPL+VAMA
Sbjct: 242 ALCLLPLFHVFGFFMLFRSISEGNTLVLMRKFDFEKMLRAVEKYRITYIPVSPPLIVAMA 301

Query: 310 KSELVAKYDLSSLQVLGCGGAPLGKEVIEKFHQKLPKVEIIQGYGLTESTAGAARSMGPE 369
           KSEL AKYDLSSLQ+L CGGAPLGKEVI+KFH K P VEIIQGYGLTES+  AAR++GPE
Sbjct: 302 KSELAAKYDLSSLQILACGGAPLGKEVIDKFHVKFPNVEIIQGYGLTESSGAAARTVGPE 361

Query: 370 ESSNTKSVGRLSENMEAMIVDPDSGEALPPGHRGELWLRGPGIMKGYVGDAKATTETLHP 429
           E S   SVGRLSENMEA IVDP SGEALPPGH+GELW+RGPGIMKGYVGD +AT ETLHP
Sbjct: 362 ECSTAGSVGRLSENMEAKIVDPSSGEALPPGHKGELWIRGPGIMKGYVGDERATAETLHP 421

Query: 430 DGWLKTGDLCYFDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSNPEIIDAAVIPYPD 489
           +GWLKTGDLCYFDSDG LYIVDRLKELIKYKAYQVPPAELEHLLQS+PEI+DAAVIPYPD
Sbjct: 422 EGWLKTGDLCYFDSDGLLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEILDAAVIPYPD 481

Query: 490 EDAGEIPMAYVVRKPGSNITEAQVIDFIAKQVAPYKKIRRVSFIDAIPKSPAGKILRREL 549
           EDAG+IP+AYVVR+ GSNI EAQV+DFIAK+VAPYKKIRRVSF++AIPKSPAGKILRREL
Sbjct: 482 EDAGQIPLAYVVRQLGSNINEAQVMDFIAKKVAPYKKIRRVSFVNAIPKSPAGKILRREL 541

Query: 550 TQHARSHGSNKL 558
            +HA S GS+KL
Sbjct: 542 VKHALSQGSSKL 553

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022923628.10.0e+0099.464-coumarate--CoA ligase-like 9 [Cucurbita moschata][more]
XP_023519270.12.2e-30898.564-coumarate--CoA ligase-like 9 [Cucurbita pepo subsp. pepo][more]
XP_023000795.13.1e-30798.384-coumarate--CoA ligase-like 9 [Cucurbita maxima][more]
XP_016899600.16.9e-26284.05PREDICTED: 4-coumarate--CoA ligase-like 9 [Cucumis melo][more]
ADN33954.12.4e-25982.434-coumarate-CoA ligase [Cucumis melo subsp. melo][more]
Match NameE-valueIdentityDescription
AT5G63380.11.8e-19263.01AMP-dependent synthetase and ligase family protein[more]
AT1G20510.17.1e-12844.38OPC-8:0 CoA ligase1[more]
AT1G20480.11.6e-12747.11AMP-dependent synthetase and ligase family protein[more]
AT1G20500.13.6e-12444.95AMP-dependent synthetase and ligase family protein[more]
AT5G38120.12.3e-11842.68AMP-dependent synthetase and ligase family protein[more]
Match NameE-valueIdentityDescription
sp|Q84P23|4CLL9_ARATH3.3e-19163.014-coumarate--CoA ligase-like 9 OS=Arabidopsis thaliana OX=3702 GN=4CLL9 PE=1 SV=... [more]
sp|Q7F1X5|4CLL5_ORYSJ7.0e-15754.264-coumarate--CoA ligase-like 5 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL5... [more]
sp|Q8RU95|4CLL6_ORYSJ8.8e-15252.154-coumarate--CoA ligase-like 6 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL6... [more]
sp|Q69RG7|4CLL7_ORYSJ2.6e-13548.914-coumarate--CoA ligase-like 7 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL7... [more]
sp|Q84P21|4CLL5_ARATH1.3e-12644.384-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
tr|A0A1S4DV63|A0A1S4DV63_CUCME4.5e-26284.054-coumarate--CoA ligase-like 9 OS=Cucumis melo OX=3656 GN=LOC103489008 PE=4 SV=1[more]
tr|E5GBV5|E5GBV5_CUCME1.6e-25982.434-coumarate-CoA ligase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
tr|A0A0A0LTS5|A0A0A0LTS5_CUCSA2.3e-25882.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G050280 PE=4 SV=1[more]
tr|A0A1S4DUD8|A0A1S4DUD8_CUCME4.4e-24177.174-coumarate--CoA ligase-like 9 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10799030... [more]
tr|A0A0A0LWL5|A0A0A0LWL5_CUCSA4.9e-24076.81Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G050300 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR020845AMP-binding_CS
IPR025110AMP-bd_C
IPR000873AMP-dep_Synth/Lig
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07589-RACarg07589-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 61..455
e-value: 6.5E-93
score: 311.5
NoneNo IPR availableGENE3DG3DSA:3.40.50.12780coord: 37..450
e-value: 5.1E-112
score: 376.7
NoneNo IPR availableGENE3DG3DSA:3.30.300.30coord: 451..553
e-value: 2.1E-28
score: 101.0
NoneNo IPR availablePANTHERPTHR24096FAMILY NOT NAMEDcoord: 22..551
NoneNo IPR availablePANTHERPTHR24096:SF2514-COUMARATE--COA LIGASE-LIKE 9coord: 22..551
NoneNo IPR availableCDDcd059044CLcoord: 48..545
e-value: 0.0
score: 700.546
NoneNo IPR availableSUPERFAMILYSSF56801Acetyl-CoA synthetase-likecoord: 59..551
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 464..539
e-value: 5.2E-14
score: 52.9
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 201..212

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg07589Carg06784Silver-seed gourdcarcarB481