Carg07474 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg07474
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	cytokinin hydroxylase-like
Location	Cucurbita_argyrosperma_scaffold_019 : 1026031 .. 1029339 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATTATGATCTTTCTAGCAACTCTTTTAGCCATCTTTGTTTCATCTTTTGCAAAGATGGCATATGATACTCTCTCTTTCTACTGGCTTACCCCTAGAAGAATCAGGAAGAGGATGGCCGAGCAAGGCGTTTATGGCCCCGACCCACGACCCTTCACTGGAAACATCCTCGATGTCGTAGAGCTTGTGAAGAAATCCACGGCTAACGACATGAGCTCCATCAGTCATGACATCGTTGATCGTTTGTTACCCTATTACTCTGCATGGACAAAGCAATACGGTACTACTAACGCTTTTATCCTGTTTAAAAGAGTTCATGATTCATTGGTTAGAAAAATATCTAGTATGATTGAGGATATTAAGCCTTTCATTACCTATAGGTACCATATTTGGATCTTTGTTAGACAAATATCTATTATCAATCTTTGCGTGACATTTACTTATTGTTAATTTTATTTTGTAATTTATTTGATTTTGTAAATAATTATAAACAGAGAACTTCATTCACTCTAATTAAGATGTGGTGGTTTTAGTAAATATTTTTGTAAACTACGAATAAACAATTAACCCGTAAACTTAAACTTATTCAGGAAAGAGGTTTATATATTGGAACGGGATGGAGCCGAGGCTGTGTTTGACGGAGATGGAGTTGGTGAAGGAAGTATTGGGGAAGGGCAGCAATGTGTGTGGGAGGTCATGGATGCAAAGACAAGGGACCAAACACTTCATAGGGAAGGGATTGTTGATGGCAAATGGAGAAGCTTGGCATCATCAACGTCACCTGATTTCACCAGCGTTTAGAGGGGAAAGGCTCAAGGTTCATATTCAAACCTGAAATATTTTATTAGATGAACACAACTCCCCACAATAATATGATATTAAGCATAAACTCTCGTGACTTTGATTTGAGCTTGCTCAAAAAGGTCTCGTACCAATAGAGATAGCATTCCTTGATTATAAACTCATGTTTCATCTAACACATTTAATATATATTTACTGTAATACGACATGAATAGAGCTACGCGGGGTTGATGGTGGAATGCACCAACAAGATGATCCAATCCTTGGAGGCCCAATTGGAGGAGGGCCAGACTGTGTTCGAAATTGCTGATTGCATGAAACGCCTCACCGCCGACATCATTTCCCGGACTGCGTTCGACACAAGCTCCGACAAAGGCATCCAAATCTTTGATATGCTCACTCAGCTTCAACACCTCTGCGCACAAGCCAGCCACCACCTCTGCATCCCCGGTAGCCGGTGCGTTTTTCTAAACCAAAAATTTCATTTCATAGAGTGCTAGGAACTACGACTCTCCATAGTGGTATGATATTATCTACTTTAAACATAAACTCGAGTGGTTTTACTTACTTATAAATCCATGATTAGCCACAATGATATGATATTGTCCACTTTGAGCATAAGCTCTCATGGTTTTGCTTTGGATTTCCCAAAAGGCCTCCTCTCAATAGTGTTAGTATTCCTCACTTATAAATCCATGATTTTCCACTAAATTAACCAACGTGGGACACTCGCTTCCAATAATCCTCAACAATGCTTCCTTCGAACAAAGTACACCATAGAGTTCTTAAGGTTTATGGAGCCCTGGAACAGTCTCTCCTTAATCGAGGCTCGACTCCTCTCTCAGTACACCCTTTGTTCAACACGTGGATCACTTTTGACTACATGTTCGAGACTCACAACCTTTTTTGTTGGTTACATTGGTGCAGGTTTTTTCCGAGTAAATACAACAGAAAAATAAAGGCACTAAAAATGGAGATGGAGAGGATGTTAATGGAGATCATTGGAAGCAGAAAAGAGGGCGTGGAGATAGGAAGAAGCAGTGGATATGGAAGCGATTTGTTGGGAATGCTATTGAAGGAGATGCAGAGAAAGAGAGACGATGGATTCAGCTTAGACTTGCAGCTCGTAATGGATGAATGCAAAACTTTCTTCTTTGCTGGCCATGATACCACTTCTCTTTTGCTCAGCTGGACCATCATGTTGCTCGCTACTAACCCTTCTTGGCAACAAAAGGCTCGGGCTGAAGTCGCCCAAGTTTGCAACGGCCGACCTCCCTCGGTCGAACATCTCCCTAAGCTCACTTTGGTAAGCCAACTAACGATTTGTTGAACATGGTTGGAAGAGAACTTAATACCACTGTAGAGAATTGTGGTTCCTAACATGATATCAGAGTCATGTTCTTAACTTAGTGTGATGTCCCGCATTGGTTGGGGATGAAAACAAAAACATCCTTTATAAGGGAGTGAAAACCTTCCCCTAGCAGATGTGTTTTAAAGCCTTGAGGGAAAGCCCGAAAGGGAAAACCCAAAGAGAACAATATCTGCTAGCGGTGGACCTGGGTCGTTACAAATGGTATTAGAGCCAGACACCGGATGATGTGCTAGCCTTCTCGCTATTCCCCAAAAGGGGGTAGACACGAGGCGGTGTGCTAGTAAGGACGTTGGGCCTCGAAGGGGTGGATTTGGAAGGGGTTCCACATCGATTGGAGAAAGGAACGAGTGCCAGTGAGGATGCTGGGCCACGAAAGGGGGTGGATTGTGATGTCCCACATTGGTTGGGGAGGAGAACAAAACACCTTTTATAAGGGTGTGGAAACCTTCCCTTAGCAGTAGCAGACGCGTTTTAAAGCTTTGAGGGGAAGCCCGAAAGGGAAAGCCCAAAAAGGACAATATCTGCTAGCGGTGGACTTGGGTTGTTACACTTAGTCATGTCAATAAAATCCTCGAACAGAGAAGTTGTGAACCTCGAATGGGTAGTCAAGTGTCGAGCCTCAATTGAGAGGAGACTGTTTGAGGGCTTTATAGGTCTCAAATGAGACTCTATAGTGTACTTTGTTCGAAGGGAGGATTGTTGATTGTTGGGATGGTCTCCATTGGTACGAGGTCTTTAGTCATAAGAGCTTATACTCAAAGTGGACAATATCATACAATTGTGGTGCGTCGTGGTTCCTAATACTTATATTTGTTTTGGTGTTGTTCTTGCAGCTGAACATGGTGATAAACGAGTCCCTAAGGCTTTACCCACCGGCCACCGTGCTACCGAGAATAGCGTTCGAGGACATAAAGGTAAGAGATTTAGAGATCCCGAAAGGGCTATCGGTATGGATACCAGTGCTTGCAATTCATCACAACGAGGAGCTGTGGGGAAAAGACGCAAACGAGTTCAACCCAGAGAGGTTTGAGTCGAAAGCATTCAGTGGCGGGCGGTTCATTCCATTTTCCAGCGGACCAAGGACCTGCATCGGCCAGGGGTTTGCGTTGATGGAAGCTAAGATCATATTGGCT

mRNA sequence

ATGGCGATTATGATCTTTCTAGCAACTCTTTTAGCCATCTTTGTTTCATCTTTTGCAAAGATGGCATATGATACTCTCTCTTTCTACTGGCTTACCCCTAGAAGAATCAGGAAGAGGATGGCCGAGCAAGGCGTTTATGGCCCCGACCCACGACCCTTCACTGGAAACATCCTCGATGTCGTAGAGCTTGTGAAGAAATCCACGGCTAACGACATGAGCTCCATCAGTCATGACATCGTTGATCCCGAGGCTGTGTTTGACGGAGATGGAGTTGGTGAAGGAAGTATTGGGGAAGGGCAGCAATGTGTGTGGGAGGTCATGGATGCAAAGACAAGGGACCAAACACTTCATAGGGAAGGGATTGCCCAATTGGAGGAGGGCCAGACTGTGTTCGAAATTGCTGATTGCATGAAACGCCTCACCGCCGACATCATTTCCCGGACTGCTGGCGGGCGGTTCATTCCATTTTCCAGCGGACCAAGGACCTGCATCGGCCAGGGGTTTGCGTTGATGGAAGCTAAGATCATATTGGCT

Coding sequence (CDS)

ATGGCGATTATGATCTTTCTAGCAACTCTTTTAGCCATCTTTGTTTCATCTTTTGCAAAGATGGCATATGATACTCTCTCTTTCTACTGGCTTACCCCTAGAAGAATCAGGAAGAGGATGGCCGAGCAAGGCGTTTATGGCCCCGACCCACGACCCTTCACTGGAAACATCCTCGATGTCGTAGAGCTTGTGAAGAAATCCACGGCTAACGACATGAGCTCCATCAGTCATGACATCGTTGATCCCGAGGCTGTGTTTGACGGAGATGGAGTTGGTGAAGGAAGTATTGGGGAAGGGCAGCAATGTGTGTGGGAGGTCATGGATGCAAAGACAAGGGACCAAACACTTCATAGGGAAGGGATTGCCCAATTGGAGGAGGGCCAGACTGTGTTCGAAATTGCTGATTGCATGAAACGCCTCACCGCCGACATCATTTCCCGGACTGCTGGCGGGCGGTTCATTCCATTTTCCAGCGGACCAAGGACCTGCATCGGCCAGGGGTTTGCGTTGATGGAAGCTAAGATCATATTGGCT

Protein sequence

MAIMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIVDPEAVFDGDGVGEGSIGEGQQCVWEVMDAKTRDQTLHREGIAQLEEGQTVFEIADCMKRLTADIISRTAGGRFIPFSSGPRTCIGQGFALMEAKIILA

Homology

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BLAST of Carg07474 vs. NCBI nr
Match: XP_022948950.1 (cytokinin hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 178.3 bits (451), Expect = 2.3e-41
Identity = 118/216 (54.63%), Postives = 127/216 (58.80%), Query Frame = 0


Query: 1   MAIMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDV 60
           MAIMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDV
Sbjct: 1   MAIMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDV 60

Query: 61  VELVKKSTANDMSSISHDIVD-----------------------------PEAVFDGDGV 120
           VELVKKSTANDMSSISHDIVD                              E     + +
Sbjct: 61  VELVKKSTANDMSSISHDIVDRLLPYYSTWTKQYGKRFIYWNGMDSRLCLTEMELVKEVL 120

Query: 121 GEGS----------------IGEGQQC----VWE-----VMDAKTRDQTLHREGI----- 150
           G+GS                IG+G        W      +  A   ++     G+     
Sbjct: 121 GKGSNVCGRSWMQRQGTKHFIGKGLLMANGEAWHHQRHLISPAFRGERLKSYAGLMVECT 180

HSP 2 Score: 63.5 bits (153), Expect = 8.1e-07
Identity = 29/30 (96.67%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 149 AGGRFIPFSSGPRTCIGQGFALMEAKIILA 179
           +GGRFIPFSSGPRTCIGQGFALMEAKIILA
Sbjct: 448 SGGRFIPFSSGPRTCIGQGFALMEAKIILA 477

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BLAST of Carg07474 vs. NCBI nr
Match: XP_023523398.1 (cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 170.2 bits (430), Expect = 6.1e-39
Identity = 114/216 (52.78%), Postives = 124/216 (57.41%), Query Frame = 0


Query: 1   MAIMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDV 60
           MAIMIFLATLL IFVSSFAK+AYDTLSFYWLTPRRIRKRMAEQGVYGP+PRPFTGNILDV
Sbjct: 1   MAIMIFLATLLVIFVSSFAKIAYDTLSFYWLTPRRIRKRMAEQGVYGPNPRPFTGNILDV 60

Query: 61  VELVKKSTANDMSSISHDIVD-----------------------------PEAVFDGDGV 120
           VELVK+STANDMSSISHDIVD                              E     + +
Sbjct: 61  VELVKESTANDMSSISHDIVDRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVL 120

Query: 121 GEGS----------------IGEGQQCV----WE-----VMDAKTRDQTLHREGI----- 150
           G+ S                IG G        W      +  A   ++     G+     
Sbjct: 121 GKSSNVCGRSWMQRQGTKHFIGNGLLMANGENWHHQRHLISPAFRGERLKSYAGLMVECT 180

HSP 2 Score: 63.5 bits (153), Expect = 8.1e-07
Identity = 29/30 (96.67%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 149 AGGRFIPFSSGPRTCIGQGFALMEAKIILA 179
           +GGRFIPFSSGPRTCIGQGFALMEAKIILA
Sbjct: 448 SGGRFIPFSSGPRTCIGQGFALMEAKIILA 477

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BLAST of Carg07474 vs. NCBI nr
Match: XP_023524594.1 (cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 170.2 bits (430), Expect = 6.1e-39
Identity = 114/216 (52.78%), Postives = 124/216 (57.41%), Query Frame = 0


Query: 1   MAIMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDV 60
           MAIMIFLATLL IFVSSFAK+AYDTLSFYWLTPRRIRKRMAEQGVYGP+PRPFTGNILDV
Sbjct: 1   MAIMIFLATLLVIFVSSFAKIAYDTLSFYWLTPRRIRKRMAEQGVYGPNPRPFTGNILDV 60

Query: 61  VELVKKSTANDMSSISHDIVD-----------------------------PEAVFDGDGV 120
           VELVK+STANDMSSISHDIVD                              E     + +
Sbjct: 61  VELVKESTANDMSSISHDIVDRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVL 120

Query: 121 GEGS----------------IGEGQQCV----WE-----VMDAKTRDQTLHREGI----- 150
           G+ S                IG G        W      +  A   ++     G+     
Sbjct: 121 GKSSNVCGRSWMQRQGTKHFIGNGLLMANGENWHHQRHLISPAFRGERLKSYAGLMVECT 180

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BLAST of Carg07474 vs. NCBI nr
Match: XP_022997665.1 (cytokinin hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 164.9 bits (416), Expect = 2.6e-37
Identity = 112/216 (51.85%), Postives = 122/216 (56.48%), Query Frame = 0


Query: 1   MAIMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDV 60
           MAIMIFLATLLAIFVSSFAK+AYDTLSFYWLTPRRIRKRMA+QGVYGPDPRPFTGNILDV
Sbjct: 1   MAIMIFLATLLAIFVSSFAKIAYDTLSFYWLTPRRIRKRMAKQGVYGPDPRPFTGNILDV 60

Query: 61  VELVKKSTANDMSSISHDIVD-----------------------------PEAVFDGDGV 120
           VELVK+S ANDMSS SHDIVD                              E     + +
Sbjct: 61  VELVKESMANDMSSTSHDIVDRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEIL 120

Query: 121 GEGS----------------IGEGQQC----VWE-----VMDAKTRDQTLHREGI----- 150
           G+ S                IG G        W      V  A   ++     G+     
Sbjct: 121 GKSSNVCGRSWMQRQGTKHFIGNGLLMANGEAWHHQRHLVSPAFRGERLKSYAGLMVECT 180

HSP 2 Score: 62.8 bits (151), Expect = 1.4e-06
Identity = 28/30 (93.33%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 149 AGGRFIPFSSGPRTCIGQGFALMEAKIILA 179
           +GGRF+PFSSGPRTCIGQGFALMEAKIILA
Sbjct: 448 SGGRFLPFSSGPRTCIGQGFALMEAKIILA 477

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BLAST of Carg07474 vs. NCBI nr
Match: XP_008461587.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])

HSP 1 Score: 117.1 bits (292), Expect = 6.2e-23
Identity = 59/81 (72.84%), Postives = 67/81 (82.72%), Query Frame = 0


Query: 1  MAIMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDV 60
          MAI+IFL TLL IF+SS AK+AYDT+S YWLTPRRIRK+M +QGV GP PRP  GNILDV
Sbjct: 1  MAIIIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61 VELVKKSTANDMSSISHDIVD 82
            L+ KSTA DMSSI H+IVD
Sbjct: 61 AALLAKSTAKDMSSIDHNIVD 81

HSP 2 Score: 58.9 bits (141), Expect = 2.0e-05
Identity = 25/30 (83.33%), Postives = 29/30 (96.67%), Query Frame = 0


Query: 149 AGGRFIPFSSGPRTCIGQGFALMEAKIILA 179
           +GGRFIPF++GPR C+GQGFALMEAKIILA
Sbjct: 452 SGGRFIPFAAGPRNCVGQGFALMEAKIILA 481

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BLAST of Carg07474 vs. TAIR10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1)

HSP 1 Score: 89.7 bits (221), Expect = 1.9e-18
Identity = 42/78 (53.85%), Postives = 57/78 (73.08%), Query Frame = 0


Query: 3  IMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVE 62
          ++  L +LL IFV++  ++ YDT+S YWLTPRRI+K M +QGV GP PRP TGNIL++  
Sbjct: 2  LLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISA 61

Query: 63 LVKKSTANDMSSISHDIV 81
          +V +S + D  SI HDIV
Sbjct: 62 MVSQSASKDCDSIHHDIV 79

HSP 2 Score: 51.2 bits (121), Expect = 7.6e-07
Identity = 23/30 (76.67%), Postives = 26/30 (86.67%), Query Frame = 0


Query: 149 AGGRFIPFSSGPRTCIGQGFALMEAKIILA 179
           +G  FIPF++GPR CIGQ FALMEAKIILA
Sbjct: 450 SGRHFIPFAAGPRNCIGQQFALMEAKIILA 479

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BLAST of Carg07474 vs. TAIR10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2)

HSP 1 Score: 72.4 bits (176), Expect = 3.2e-13
Identity = 35/80 (43.75%), Postives = 54/80 (67.50%), Query Frame = 0


Query: 1  MAIMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDV 60
          M + + L  +L I ++   ++ YD++  Y+LTPRRI+K M  QG+ GP PR  TGNI+D+
Sbjct: 1  MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 61 VELVKKSTANDMSSISHDIV 81
           +++  S +ND SSI H+IV
Sbjct: 61 SKMLSHSASNDCSSIHHNIV 80

HSP 2 Score: 47.4 bits (111), Expect = 1.1e-05
Identity = 20/26 (76.92%), Postives = 24/26 (92.31%), Query Frame = 0


Query: 153 FIPFSSGPRTCIGQGFALMEAKIILA 179
           F+PF++GPR CIGQ FA+MEAKIILA
Sbjct: 448 FMPFAAGPRNCIGQTFAMMEAKIILA 473

[Close]

BLAST of Carg07474 vs. TAIR10
Match: AT1G17060.1 (cytochrome p450 72c1)

HSP 1 Score: 47.0 bits (110), Expect = 1.4e-05
Identity = 18/26 (69.23%), Postives = 24/26 (92.31%), Query Frame = 0


Query: 153 FIPFSSGPRTCIGQGFALMEAKIILA 179
           F+PFSSGPRTCIGQ F++++AK+ LA
Sbjct: 414 FLPFSSGPRTCIGQNFSMLQAKLFLA 439

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BLAST of Carg07474 vs. TAIR10
Match: AT3G14630.1 (cytochrome P450, family 72, subfamily A, polypeptide 9)

HSP 1 Score: 42.7 bits (99), Expect = 2.7e-04
Identity = 18/26 (69.23%), Postives = 21/26 (80.77%), Query Frame = 0


Query: 153 FIPFSSGPRTCIGQGFALMEAKIILA 179
           F+PF  GPR CIGQ FAL+EAK+ LA
Sbjct: 446 FLPFGWGPRICIGQNFALLEAKMALA 471

[Close]

BLAST of Carg07474 vs. TAIR10
Match: AT3G53130.1 (Cytochrome P450 superfamily protein)

HSP 1 Score: 42.7 bits (99), Expect = 2.7e-04
Identity = 18/27 (66.67%), Postives = 21/27 (77.78%), Query Frame = 0


Query: 152 RFIPFSSGPRTCIGQGFALMEAKIILA 179
           +FIPFS GPR C+G  FALMEA + LA
Sbjct: 476 KFIPFSGGPRKCVGDQFALMEAIVALA 502

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BLAST of Carg07474 vs. Swiss-Prot
Match: sp|Q9FF18|C7351_ARATH (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 3.5e-17
Identity = 42/78 (53.85%), Postives = 57/78 (73.08%), Query Frame = 0


Query: 3  IMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVE 62
          ++  L +LL IFV++  ++ YDT+S YWLTPRRI+K M +QGV GP PRP TGNIL++  
Sbjct: 2  LLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISA 61

Query: 63 LVKKSTANDMSSISHDIV 81
          +V +S + D  SI HDIV
Sbjct: 62 MVSQSASKDCDSIHHDIV 79

HSP 2 Score: 51.2 bits (121), Expect = 1.4e-05
Identity = 23/30 (76.67%), Postives = 26/30 (86.67%), Query Frame = 0


Query: 149 AGGRFIPFSSGPRTCIGQGFALMEAKIILA 179
           +G  FIPF++GPR CIGQ FALMEAKIILA
Sbjct: 450 SGRHFIPFAAGPRNCIGQQFALMEAKIILA 479

[Close]

BLAST of Carg07474 vs. Swiss-Prot
Match: sp|Q9ZW95|C7352_ARATH (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 5.7e-12
Identity = 35/80 (43.75%), Postives = 54/80 (67.50%), Query Frame = 0


Query: 1  MAIMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDV 60
          M + + L  +L I ++   ++ YD++  Y+LTPRRI+K M  QG+ GP PR  TGNI+D+
Sbjct: 1  MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 61 VELVKKSTANDMSSISHDIV 81
           +++  S +ND SSI H+IV
Sbjct: 61 SKMLSHSASNDCSSIHHNIV 80

HSP 2 Score: 47.4 bits (111), Expect = 2.0e-04
Identity = 20/26 (76.92%), Postives = 24/26 (92.31%), Query Frame = 0


Query: 153 FIPFSSGPRTCIGQGFALMEAKIILA 179
           F+PF++GPR CIGQ FA+MEAKIILA
Sbjct: 448 FMPFAAGPRNCIGQTFAMMEAKIILA 473

[Close]

BLAST of Carg07474 vs. Swiss-Prot
Match: sp|Q9SHG5|C72C1_ARATH (Cytochrome P450 72C1 OS=Arabidopsis thaliana OX=3702 GN=CYP72C1 PE=2 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 2.6e-04
Identity = 18/26 (69.23%), Postives = 24/26 (92.31%), Query Frame = 0


Query: 153 FIPFSSGPRTCIGQGFALMEAKIILA 179
           F+PFSSGPRTCIGQ F++++AK+ LA
Sbjct: 457 FLPFSSGPRTCIGQNFSMLQAKLFLA 482

[Close]

BLAST of Carg07474 vs. Swiss-Prot
Match: sp|Q99N16|CP4F3_MOUSE (Leukotriene-B(4) omega-hydroxylase 2 OS=Mus musculus OX=10090 GN=Cyp4f3 PE=1 SV=2)

HSP 1 Score: 45.4 bits (106), Expect = 7.5e-04
Identity = 21/40 (52.50%), Postives = 26/40 (65.00%), Query Frame = 0


Query: 139 RLTADIISRTAGGRFIPFSSGPRTCIGQGFALMEAKIILA 179
           R  AD +   +   FIPFS+GPR CIGQ FA+ E K+ LA
Sbjct: 444 RFDADNVKGRSPLAFIPFSAGPRNCIGQTFAMSEMKVALA 483

[Close]

BLAST of Carg07474 vs. Swiss-Prot
Match: sp|Q9HBI6|CP4FB_HUMAN (Phylloquinone omega-hydroxylase CYP4F11 OS=Homo sapiens OX=9606 GN=CYP4F11 PE=1 SV=3)

HSP 1 Score: 45.4 bits (106), Expect = 7.5e-04
Identity = 18/26 (69.23%), Postives = 22/26 (84.62%), Query Frame = 0


Query: 153 FIPFSSGPRTCIGQGFALMEAKIILA 179
           FIPFS+GPR CIGQ FA+ E K++LA
Sbjct: 458 FIPFSAGPRNCIGQAFAMAEMKVVLA 483

[Close]

BLAST of Carg07474 vs. TrEMBL
Match: tr|A0A1S3CGC0|A0A1S3CGC0_CUCME (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.1e-23
Identity = 59/81 (72.84%), Postives = 67/81 (82.72%), Query Frame = 0


Query: 1  MAIMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDV 60
          MAI+IFL TLL IF+SS AK+AYDT+S YWLTPRRIRK+M +QGV GP PRP  GNILDV
Sbjct: 1  MAIIIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61 VELVKKSTANDMSSISHDIVD 82
            L+ KSTA DMSSI H+IVD
Sbjct: 61 AALLAKSTAKDMSSIDHNIVD 81

HSP 2 Score: 58.9 bits (141), Expect = 1.3e-05
Identity = 25/30 (83.33%), Postives = 29/30 (96.67%), Query Frame = 0


Query: 149 AGGRFIPFSSGPRTCIGQGFALMEAKIILA 179
           +GGRFIPF++GPR C+GQGFALMEAKIILA
Sbjct: 452 SGGRFIPFAAGPRNCVGQGFALMEAKIILA 481

[Close]

BLAST of Carg07474 vs. TrEMBL
Match: tr|A0A0A0L6Y6|A0A0A0L6Y6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=4 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 9.1e-23
Identity = 59/81 (72.84%), Postives = 66/81 (81.48%), Query Frame = 0


Query: 1  MAIMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDV 60
          MAIMI L TLL IF+SS AK+AYDT+S YWLTPRRIRK+M +QGV GP PRP  GNILDV
Sbjct: 1  MAIMIILTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61 VELVKKSTANDMSSISHDIVD 82
            L+ KSTA DMSSI H+IVD
Sbjct: 61 AALLGKSTAKDMSSIDHNIVD 81

HSP 2 Score: 59.3 bits (142), Expect = 1.0e-05
Identity = 26/30 (86.67%), Postives = 29/30 (96.67%), Query Frame = 0


Query: 149 AGGRFIPFSSGPRTCIGQGFALMEAKIILA 179
           +GGRFIPF++GPR CIGQGFALMEAKIILA
Sbjct: 452 SGGRFIPFAAGPRNCIGQGFALMEAKIILA 481

[Close]

BLAST of Carg07474 vs. TrEMBL
Match: tr|A0A1Q3C6F9|A0A1Q3C6F9_CEPFO (p450 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_19141 PE=4 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 6.8e-18
Identity = 48/78 (61.54%), Postives = 58/78 (74.36%), Query Frame = 0


Query: 4  MIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVEL 63
          M+ L  LL +F+S F K+AYDTLS YW+TPRRI+K M  QGV GP PRP  GNILD+   
Sbjct: 5  MVMLTPLLVMFLSLFIKVAYDTLSCYWITPRRIKKIMERQGVRGPKPRPLIGNILDMASF 64

Query: 64 VKKSTANDMSSISHDIVD 82
          V +ST+ DM+SI HDIVD
Sbjct: 65 VSQSTSKDMNSIHHDIVD 82

HSP 2 Score: 48.9 bits (115), Expect = 1.4e-02
Identity = 20/29 (68.97%), Postives = 25/29 (86.21%), Query Frame = 0


Query: 150 GGRFIPFSSGPRTCIGQGFALMEAKIILA 179
           G  FIPF++GPR CIGQ FA+MEAK+IL+
Sbjct: 452 GRHFIPFAAGPRNCIGQSFAMMEAKLILS 480

[Close]

BLAST of Carg07474 vs. TrEMBL
Match: tr|A0A2C9WD27|A0A2C9WD27_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_02G068100 PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 4.4e-17
Identity = 49/79 (62.03%), Postives = 60/79 (75.95%), Query Frame = 0


Query: 3  IMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVE 62
          +++ L +LL IF+S F K+AYDT+S Y+LTPRRI+K M +QGV GP PRP TGNILDV  
Sbjct: 2  VILVLISLLIIFLSLFFKIAYDTISCYFLTPRRIKKIMEKQGVRGPKPRPLTGNILDVTS 61

Query: 63 LVKKSTANDMSSISHDIVD 82
           V  ST+ DM SI HDIVD
Sbjct: 62 FVSLSTSKDMDSIHHDIVD 80

HSP 2 Score: 50.1 bits (118), Expect = 6.1e-03
Identity = 22/29 (75.86%), Postives = 25/29 (86.21%), Query Frame = 0


Query: 150 GGRFIPFSSGPRTCIGQGFALMEAKIILA 179
           G  FIPF++GPR CIGQ FA+MEAKIILA
Sbjct: 450 GRHFIPFAAGPRNCIGQTFAMMEAKIILA 478

[Close]

BLAST of Carg07474 vs. TrEMBL
Match: tr|A0A1J7HXJ3|A0A1J7HXJ3_LUPAN (Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_26690 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 5.7e-17
Identity = 47/79 (59.49%), Postives = 59/79 (74.68%), Query Frame = 0


Query: 3  IMIFLATLLAIFVSSFAKMAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVE 62
          +M+   TLL IFV+   K+AYDT+SFYWLTPRRI+K M +QGV+GP PR  TGNI D+  
Sbjct: 4  VMVVFTTLLVIFVTLVFKVAYDTISFYWLTPRRIKKIMDKQGVHGPKPRFITGNITDMAS 63

Query: 63 LVKKSTANDMSSISHDIVD 82
          LV  S + DM++ISHD VD
Sbjct: 64 LVSTSISQDMNTISHDTVD 82

HSP 2 Score: 53.5 bits (127), Expect = 5.5e-04
Identity = 23/28 (82.14%), Postives = 26/28 (92.86%), Query Frame = 0


Query: 151 GRFIPFSSGPRTCIGQGFALMEAKIILA 179
           GRFIPF+SGPR C+GQ FA+MEAKIILA
Sbjct: 448 GRFIPFASGPRNCVGQTFAIMEAKIILA 475

The following BLAST results are available for this feature:

BLAST of Carg07474 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948950.1	2.3e-41	54.63	cytokinin hydroxylase-like [Cucurbita moschata]	[more]
XP_023523398.1	6.1e-39	52.78	cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023524594.1	6.1e-39	52.78	cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022997665.1	2.6e-37	51.85	cytokinin hydroxylase-like [Cucurbita maxima]	[more]
XP_008461587.1	6.2e-23	72.84	PREDICTED: cytokinin hydroxylase-like [Cucumis melo]	[more]

BLAST of Carg07474 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G38450.1	1.9e-18	53.85	cytochrome P450, family 735, subfamily A, polypeptide 1	[more]
AT1G67110.1	3.2e-13	43.75	cytochrome P450, family 735, subfamily A, polypeptide 2	[more]
AT1G17060.1	1.4e-05	69.23	cytochrome p450 72c1	[more]
AT3G14630.1	2.7e-04	69.23	cytochrome P450, family 72, subfamily A, polypeptide 9	[more]
AT3G53130.1	2.7e-04	66.67	Cytochrome P450 superfamily protein	[more]

BLAST of Carg07474 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9FF18\|C7351_ARATH	3.5e-17	53.85	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1	[more]
sp\|Q9ZW95\|C7352_ARATH	5.7e-12	43.75	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1	[more]
sp\|Q9SHG5\|C72C1_ARATH	2.6e-04	69.23	Cytochrome P450 72C1 OS=Arabidopsis thaliana OX=3702 GN=CYP72C1 PE=2 SV=2	[more]
sp\|Q99N16\|CP4F3_MOUSE	7.5e-04	52.50	Leukotriene-B(4) omega-hydroxylase 2 OS=Mus musculus OX=10090 GN=Cyp4f3 PE=1 SV=...	[more]
sp\|Q9HBI6\|CP4FB_HUMAN	7.5e-04	69.23	Phylloquinone omega-hydroxylase CYP4F11 OS=Homo sapiens OX=9606 GN=CYP4F11 PE=1 ...	[more]

BLAST of Carg07474 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3CGC0\|A0A1S3CGC0_CUCME	4.1e-23	72.84	cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=4 SV=1	[more]
tr\|A0A0A0L6Y6\|A0A0A0L6Y6_CUCSA	9.1e-23	72.84	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=4 SV=1	[more]
tr\|A0A1Q3C6F9\|A0A1Q3C6F9_CEPFO	6.8e-18	61.54	p450 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_191...	[more]
tr\|A0A2C9WD27\|A0A2C9WD27_MANES	4.4e-17	62.03	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_02G068100 PE=4 SV=...	[more]
tr\|A0A1J7HXJ3\|A0A1J7HXJ3_LUPAN	5.7e-17	59.49	Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_26690 PE=4 S...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506	iron ion binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR017972	Cyt_P450_CS
IPR001128	Cyt_P450
IPR036396	Cyt_P450_sf

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg07474-RA	Carg07474-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	G3DSA:1.10.630.10		coord: 146..178 e-value: 3.5E-10 score: 41.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	SSF48264	Cytochrome P450	coord: 146..178
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 151..178 e-value: 2.9E-8 score: 32.8
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 7..81
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 152..178
None	No IPR available	PANTHER	PTHR24282:SF56	CYTOKININ HYDROXYLASE	coord: 152..178
None	No IPR available	PANTHER	PTHR24282:SF56	CYTOKININ HYDROXYLASE	coord: 7..81
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 156..165

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg07474	Cp4.1LG02g17240	Cucurbita pepo (Zucchini)	carcpeB1072
Carg07474	Cla97C05G093780	Watermelon (97103) v2	carwmbB0795
Carg07474	Bhi01G001461	Wax gourd	carwgoB0896

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg07474	Carg22273	Silver-seed gourd	carcarB471

The following block(s) are covering this gene:


Gene	Organism	Block
Carg07474	Bottle gourd (USVL1VR-Ls)	carlsiB700
Carg07474	Melon (DHL92) v3.5.1	carmeB0757
Carg07474	Melon (DHL92) v3.6.1	carmedB0772
Carg07474	Watermelon (Charleston Gray)	carwcgB0739
Carg07474	Watermelon (97103) v1	carwmB0760
Carg07474	Watermelon (97103) v1	carwmB0761
Carg07474	Cucumber (Gy14) v2	carcgybB0757
Carg07474	Cucumber (Gy14) v1	carcgyB0854
Carg07474	Cucurbita maxima (Rimu)	carcmaB1057
Carg07474	Cucurbita maxima (Rimu)	carcmaB1059
Carg07474	Cucurbita moschata (Rifu)	carcmoB1031
Carg07474	Cucurbita moschata (Rifu)	carcmoB1033
Carg07474	Cucurbita pepo (Zucchini)	carcpeB1073
Carg07474	Wild cucumber (PI 183967)	carcpiB0798
Carg07474	Cucumber (Chinese Long) v2	carcuB0780
Carg07474	Cucumber (Chinese Long) v3	carcucB0801