Carg07216 (gene) Silver-seed gourd

Overview
NameCarg07216
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionSentrin-specific protease 8
LocationCucurbita_argyrosperma_scaffold_017 : 696167 .. 696841 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCTAAATTCTTGACTTACGGTGATGTCGTTATTCGAGGATCCGATTTGCATAGTTTAGAAGGCAGAAACTACTTGAACGACACCATCATAGATTTCTACTTTGCCTACCTCTCCGCAACGTACCAATCGAACGAAATTCTATCGATACCAACGAGTGTCTCTTACTTGTTGGGCAACACTCTTGATTGGGATATTTTTATATCCACGATCGAATCCCTGAACCTCCATGAAAAGAAGCTAATTTTTTTCGTCATTAATGACAACTCCAACGTCTCCTCTTCCGGTGGATCTCATTGGAGCTTGCTGGTGTATTGCAGAAAACGGAATTTATTCATGCACTACGACAGCCTTTCCACGATGAACAGTGAACCTGCTTTCACCCTTTATGAATCTGTGAGTGGCTACATCAATTATGATCCAGCAGAGTTTATGGAATGTCGTGCACCTCGACAAAATAATGGATATGATTGTGGACTATATGTGATGGAAACCGCTCGGGTGATTTACGAGCATTATTTCAACGTTGATCAATTTCGAAGCAAGGACGAGATTTCTGGAACCATTAATGTGATGCGAAGTGTAAAGAGTAAGTATGTTGAGTCCAACATGCGTTCTGAAATGATGCAGCTTATTCGCAGATTGAAGCAAGAACAAATCAACCCTATATAG

mRNA sequence

ATGGCTGCTAAATTCTTGACTTACGGTGATGTCGTTATTCGAGGATCCGATTTGCATAGTTTAGAAGGCAGAAACTACTTGAACGACACCATCATAGATTTCTACTTTGCCTACCTCTCCGCAACGTACCAATCGAACGAAATTCTATCGATACCAACGAGTGTCTCTTACTTGTTGGGCAACACTCTTGATTGGGATATTTTTATATCCACGATCGAATCCCTGAACCTCCATGAAAAGAAGCTAATTTTTTTCGTCATTAATGACAACTCCAACGTCTCCTCTTCCGGTGGATCTCATTGGAGCTTGCTGGTGTATTGCAGAAAACGGAATTTATTCATGCACTACGACAGCCTTTCCACGATGAACAGTGAACCTGCTTTCACCCTTTATGAATCTGTGAGTGGCTACATCAATTATGATCCAGCAGAGTTTATGGAATGTCGTGCACCTCGACAAAATAATGGATATGATTGTGGACTATATGTGATGGAAACCGCTCGGGTGATTTACGAGCATTATTTCAACGTTGATCAATTTCGAAGCAAGGACGAGATTTCTGGAACCATTAATGTGATGCGAAGTGTAAAGAGTAAGTATGTTGAGTCCAACATGCGTTCTGAAATGATGCAGCTTATTCGCAGATTGAAGCAAGAACAAATCAACCCTATATAG

Coding sequence (CDS)

ATGGCTGCTAAATTCTTGACTTACGGTGATGTCGTTATTCGAGGATCCGATTTGCATAGTTTAGAAGGCAGAAACTACTTGAACGACACCATCATAGATTTCTACTTTGCCTACCTCTCCGCAACGTACCAATCGAACGAAATTCTATCGATACCAACGAGTGTCTCTTACTTGTTGGGCAACACTCTTGATTGGGATATTTTTATATCCACGATCGAATCCCTGAACCTCCATGAAAAGAAGCTAATTTTTTTCGTCATTAATGACAACTCCAACGTCTCCTCTTCCGGTGGATCTCATTGGAGCTTGCTGGTGTATTGCAGAAAACGGAATTTATTCATGCACTACGACAGCCTTTCCACGATGAACAGTGAACCTGCTTTCACCCTTTATGAATCTGTGAGTGGCTACATCAATTATGATCCAGCAGAGTTTATGGAATGTCGTGCACCTCGACAAAATAATGGATATGATTGTGGACTATATGTGATGGAAACCGCTCGGGTGATTTACGAGCATTATTTCAACGTTGATCAATTTCGAAGCAAGGACGAGATTTCTGGAACCATTAATGTGATGCGAAGTGTAAAGAGTAAGTATGTTGAGTCCAACATGCGTTCTGAAATGATGCAGCTTATTCGCAGATTGAAGCAAGAACAAATCAACCCTATATAG

Protein sequence

MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLSTMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQFRSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQINPI
Homology
[Close]
BLAST of Carg07216 vs. NCBI nr
Match: XP_022953908.1 (NEDD8-specific protease 1-like [Cucurbita moschata])

HSP 1 Score: 434.9 bits (1117), Expect = 1.7e-118
Identity = 215/220 (97.73%), Postives = 218/220 (99.09%), Query Frame = 0 

Query: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60
           MAAKFLTYGDVVI+G DLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVS+LLG
Sbjct: 1   MAAKFLTYGDVVIQGFDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLG 60

Query: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120
           NTLDWDIFISTIESLNLHEKKLIFFV+NDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS
Sbjct: 61  NTLDWDIFISTIESLNLHEKKLIFFVVNDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120

Query: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180
           TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF
Sbjct: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180

Query: 181 RSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQ 221
           RSKDEISG INVMRSVKSKYVESNMRSEMMQLIRRLKQEQ
Sbjct: 181 RSKDEISGPINVMRSVKSKYVESNMRSEMMQLIRRLKQEQ 220

[Close]
BLAST of Carg07216 vs. NCBI nr
Match: XP_023549404.1 (NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 418.3 bits (1074), Expect = 1.6e-113
Identity = 208/222 (93.69%), Postives = 214/222 (96.40%), Query Frame = 0 

Query: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60
           MAAKFLTYGDVVI+GSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVS+LLG
Sbjct: 1   MAAKFLTYGDVVIQGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSFLLG 60

Query: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120
           NTLDWDIFISTIESLNL EKKLIFFV+NDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS
Sbjct: 61  NTLDWDIFISTIESLNLPEKKLIFFVVNDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120

Query: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180
           TMNSE AFTLYESV+GYINY+PAEFMECR PRQNNGYDCGLYVMETARVIYEHY NVDQF
Sbjct: 121 TMNSESAFTLYESVNGYINYEPAEFMECRTPRQNNGYDCGLYVMETARVIYEHYCNVDQF 180

Query: 181 RSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQIN 223
           RSKDEISG INVMRSVKSKYVE NMRSEM+QLI  LKQEQIN
Sbjct: 181 RSKDEISGPINVMRSVKSKYVEFNMRSEMLQLIHGLKQEQIN 222

[Close]
BLAST of Carg07216 vs. NCBI nr
Match: XP_022988745.1 (NEDD8-specific protease 1-like [Cucurbita maxima])

HSP 1 Score: 329.7 bits (844), Expect = 7.6e-87
Identity = 159/218 (72.94%), Postives = 182/218 (83.49%), Query Frame = 0 

Query: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60
           M  KFLTY  VV+R SD+ SLE  NYLND IIDFYFAYL  +YQS++IL +PTS+S+LLG
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLVPTSISFLLG 60

Query: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120
           N  DWD FI+T+ESLN HEKKLIFF +NDNSN SSSGGSHWSLLVYCRKRNLFMHYDSL 
Sbjct: 61  NAPDWDTFIATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH 120

Query: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180
            MN+E AF +YESV+ YI+YD AEFMECRAPRQ NGYDCGLYVME ARVIYE YFN DQF
Sbjct: 121 MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNGYDCGLYVMEIARVIYERYFNFDQF 180

Query: 181 RSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQ 219
           +++DEI G+ NV++ V  K+VESNMRSEM+QLI  L Q
Sbjct: 181 QNEDEICGSFNVVQRVNCKHVESNMRSEMLQLILGLNQ 218

[Close]
BLAST of Carg07216 vs. NCBI nr
Match: XP_022942008.1 (NEDD8-specific protease 1-like [Cucurbita moschata])

HSP 1 Score: 321.2 bits (822), Expect = 2.7e-84
Identity = 153/212 (72.17%), Postives = 177/212 (83.49%), Query Frame = 0 

Query: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60
           M  KFLTY  VV+R SD+ SLE  NYLND IIDFYFAYL  +YQS++IL I TS+S+LLG
Sbjct: 1   MDPKFLTYNGVVLRRSDVRSLEDCNYLNDAIIDFYFAYLFESYQSDDILLISTSISFLLG 60

Query: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120
           N  DWD F++T+ESLN HEKKLIFF +NDNSN SSSGGSHWSLLVYCRKRNLFMHYDSL 
Sbjct: 61  NASDWDTFVATVESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHYDSLH 120

Query: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYFNVDQF 180
            MN+E AF +YESV+ YI+YD AEFMECRAPRQ N YDCGLYVME ARVIYEHYFN DQF
Sbjct: 121 MMNNESAFAIYESVNSYISYDSAEFMECRAPRQRNEYDCGLYVMEIARVIYEHYFNFDQF 180

Query: 181 RSKDEISGTINVMRSVKSKYVESNMRSEMMQL 213
           +++DEI G+ NV++SV  K+VESNMRSE + +
Sbjct: 181 QNEDEICGSFNVVQSVNCKHVESNMRSESINI 212

[Close]
BLAST of Carg07216 vs. NCBI nr
Match: XP_023526001.1 (NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 253.1 bits (645), Expect = 9.1e-64
Identity = 118/160 (73.75%), Postives = 136/160 (85.00%), Query Frame = 0 

Query: 1   MAAKFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLG 60
           M  KFLTY  VV+R  D+ SLE  NYLND IIDFYFAYL  ++QS++IL +PTS+S+LLG
Sbjct: 1   MDPKFLTYNGVVLRRFDVRSLEDCNYLNDVIIDFYFAYLFESHQSDDILLLPTSISFLLG 60

Query: 61  NTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLS 120
           N LDWD F++T+ESLN HEKKLIFF +NDNSN SSSGGSHWSLLVYCRKRNLFMH+DSL 
Sbjct: 61  NALDWDTFMATMESLNFHEKKLIFFTVNDNSNFSSSGGSHWSLLVYCRKRNLFMHHDSLH 120

Query: 121 TMNSEPAFTLYESVSGYINYDPAEFMECRAPRQNNGYDCG 161
            MN+E AFT+YESV+ YI+YD AEFMECRAPRQ NGYDCG
Sbjct: 121 MMNNESAFTIYESVNSYISYDSAEFMECRAPRQRNGYDCG 160

[Close]
BLAST of Carg07216 vs. TAIR10
Match: AT5G60190.1 (Cysteine proteinases superfamily protein)

HSP 1 Score: 156.4 bits (394), Expect = 2.1e-38
Identity = 86/221 (38.91%), Postives = 139/221 (62.90%), Query Frame = 0 

Query: 4   KFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTL 63
           K L+Y DVV+R SDL  L G  +LND +I+FY ++LS  + S  I  IP S+++ + N  
Sbjct: 9   KILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISNCP 68

Query: 64  DWDIFISTIESLNLHEKKLIFFVINDNSNVS-SSGGSHWSLLVYCRKRNLFMHYDSLSTM 123
           D +     ++ LNL +K L+   +NDNSNV  + GG HWSLLVY ++ N F+H+DS   +
Sbjct: 69  DTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFVHHDSYMGV 128

Query: 124 NSEPAFTLYESVSGYINYDPAEFMECR-APRQNNGYDCGLYVMETARVIYEHYFNVDQFR 183
           N   A  L+++VS +++   A + EC   P+Q NGYDCG++++ TARVI E +F+    +
Sbjct: 129 NRWSAKQLFKAVSPFVSNGDASYKECTDTPQQKNGYDCGVFLLATARVICE-WFSSGGMK 188

Query: 184 SKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQIN 223
           ++DE+    NV  +V    + +++R E++ LI++L  E ++
Sbjct: 189 NRDEL-WFANVKETVPD--LVNHLREEILALIKKLMSESVS 225

[Close]
BLAST of Carg07216 vs. TAIR10
Match: AT4G33620.1 (Cysteine proteinases superfamily protein)

HSP 1 Score: 50.1 bits (118), Expect = 2.1e-06
Identity = 46/203 (22.66%), Postives = 74/203 (36.45%), Query Frame = 0 

Query: 11  VVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNE--------------ILSIPTSVS 70
           VV+R  D+  L+ R ++NDTIIDFY  YL       E              + ++     
Sbjct: 305 VVVRKQDIELLKPRRFINDTIIDFYIKYLKNRISPKERGRFHFFNCFFFRKLANLDKGTP 364

Query: 71  YLLGNTLDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHY 130
              G    +       ++++L EK  IF  IN +         HWSL++ C    L   +
Sbjct: 365 STCGGREAYQRVQKWTKNVDLFEKDYIFIPINCS--------FHWSLVIICHPGELVPSH 424

Query: 131 DSLSTMNSE-------PAFTLYESV-----SGYINYDPAEFME----------------- 165
            +  + + E       P     +S+      G IN  P+   E                 
Sbjct: 425 VNFHSFDDEVENPQRVPCILHLDSIKGSHKGGLINIFPSYLREEWKARHENTTNDSSRAP 484

[Close]
BLAST of Carg07216 vs. TAIR10
Match: AT1G09730.1 (Cysteine proteinases superfamily protein)

HSP 1 Score: 45.1 bits (105), Expect = 6.8e-05
Identity = 46/191 (24.08%), Postives = 71/191 (37.17%), Query Frame = 0 

Query: 17  DLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTLDWDIFISTI---- 76
           D+  L+   ++NDTIIDFY  YL    Q+ E        S+      D D   S+I    
Sbjct: 432 DVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPSSIADGK 491

Query: 77  ----------ESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYC--------------- 136
                       +++  K  IF  +N N         HWSL+V C               
Sbjct: 492 AAFLRVRKWTRKVDMFGKDYIFVPVNYN--------LHWSLIVICHPGEVANRTDLDLDD 551

Query: 137 -RKRNLFMHYDSL-----------STMNSEPAFTLYESVSGYIN--YDPAEFMECRAPRQ 165
            +K    +H DS+            T   E     ++  S  I+  +    F+    P+Q
Sbjct: 552 SKKVPCILHMDSIKGSHAGLKNLVQTYLCEEWKERHKETSDDISSRFMNLRFVSLELPQQ 611

[Close]
BLAST of Carg07216 vs. Swiss-Prot
Match: sp|Q9LSS7|RUBP1_ARATH (NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.8e-37
Identity = 86/221 (38.91%), Postives = 139/221 (62.90%), Query Frame = 0 

Query: 4   KFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTL 63
           K L+Y DVV+R SDL  L G  +LND +I+FY ++LS  + S  I  IP S+++ + N  
Sbjct: 9   KILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISNCP 68

Query: 64  DWDIFISTIESLNLHEKKLIFFVINDNSNVS-SSGGSHWSLLVYCRKRNLFMHYDSLSTM 123
           D +     ++ LNL +K L+   +NDNSNV  + GG HWSLLVY ++ N F+H+DS   +
Sbjct: 69  DTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFVHHDSYMGV 128

Query: 124 NSEPAFTLYESVSGYINYDPAEFMECR-APRQNNGYDCGLYVMETARVIYEHYFNVDQFR 183
           N   A  L+++VS +++   A + EC   P+Q NGYDCG++++ TARVI E +F+    +
Sbjct: 129 NRWSAKQLFKAVSPFVSNGDASYKECTDTPQQKNGYDCGVFLLATARVICE-WFSSGGMK 188

Query: 184 SKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRLKQEQIN 223
           ++DE+    NV  +V    + +++R E++ LI++L  E ++
Sbjct: 189 NRDEL-WFANVKETVPD--LVNHLREEILALIKKLMSESVS 225

[Close]
BLAST of Carg07216 vs. Swiss-Prot
Match: sp|Q5FVJ8|SENP8_RAT (Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-23
Identity = 65/175 (37.14%), Postives = 99/175 (56.57%), Query Frame = 0 

Query: 6   LTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATY---QSNEILSIPTSVSYLLGNT 65
           L+Y D ++R SD+  L+  N+LND II F F Y +++     S+ +  I   V+  +  T
Sbjct: 6   LSYMDSLLRQSDVSLLDPPNWLNDHIIGFAFEYFASSQFHDCSDHVCFISPEVTQFIKCT 65

Query: 66  LDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLSTM 125
                    +E L+L  K+++F  INDNSN  ++GG+HWSLLVY + +N F HYDS S  
Sbjct: 66  SSPAEIAMFLEPLDLPHKRVVFLAINDNSN-QAAGGTHWSLLVYLQDKNSFFHYDSHSRS 125

Query: 126 NSEPAFTLYESVSGYINY--DPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYF 176
           NS  A  + E +  ++    D   F+E +AP Q N YDCG+YV+     + ++ F
Sbjct: 126 NSIHAKQVAEKLKAFLGSKGDKLVFVEEKAPAQQNSYDCGMYVICNTEALCQNLF 179

[Close]
BLAST of Carg07216 vs. Swiss-Prot
Match: sp|Q96LD8|SENP8_HUMAN (Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.8e-23
Identity = 64/175 (36.57%), Postives = 100/175 (57.14%), Query Frame = 0 

Query: 6   LTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATY---QSNEILSIPTSVSYLLGNT 65
           L+Y D ++R SD+  L+  ++LND II F F Y + +     S+ +  I   V+  +  T
Sbjct: 6   LSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQFIKCT 65

Query: 66  LDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLSTM 125
            +       +E L+L  K+++F  INDNSN  ++GG+HWSLLVY + +N F HYDS S  
Sbjct: 66  SNPAEIAMFLEPLDLPNKRVVFLAINDNSN-QAAGGTHWSLLVYLQDKNSFFHYDSHSRS 125

Query: 126 NSEPAFTLYESVSGYINY--DPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYF 176
           NS  A  + E +  ++    D   F+E +AP Q N YDCG+YV+     + +++F
Sbjct: 126 NSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNSYDCGMYVICNTEALCQNFF 179

[Close]
BLAST of Carg07216 vs. Swiss-Prot
Match: sp|Q9D2Z4|SENP8_MOUSE (Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 1.5e-22
Identity = 64/175 (36.57%), Postives = 97/175 (55.43%), Query Frame = 0 

Query: 6   LTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATY---QSNEILSIPTSVSYLLGNT 65
           L+Y D ++R SD+  L+  ++LND II F F Y + +     S+ +  I   V+  +  T
Sbjct: 6   LSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVCFISPEVTQFIKCT 65

Query: 66  LDWDIFISTIESLNLHEKKLIFFVINDNSNVSSSGGSHWSLLVYCRKRNLFMHYDSLSTM 125
                    +E L+L  K+++F  INDNSN  ++GG+HWSLLVY + +N F HYDS S  
Sbjct: 66  SSPAEIAMFLEPLDLPHKRVVFLAINDNSN-QAAGGTHWSLLVYLQDKNSFFHYDSHSRS 125

Query: 126 NSEPAFTLYESVSGYINY--DPAEFMECRAPRQNNGYDCGLYVMETARVIYEHYF 176
           NS  A  + E +  ++    D   F+E +AP Q N YDCG+YV+     + +  F
Sbjct: 126 NSIHAKQVAEKLKAFLGSKGDKLVFVEEKAPAQENSYDCGMYVICNTEALCQSLF 179

[Close]
BLAST of Carg07216 vs. Swiss-Prot
Match: sp|Q54XR2|SENP8_DICDI (Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=senp8 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 6.7e-18
Identity = 54/174 (31.03%), Postives = 94/174 (54.02%), Query Frame = 0 

Query: 6   LTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTL-- 65
           ++Y D  I  SD++ L+   +LND+II FY  +L    + N+   IP  V  L  + +  
Sbjct: 7   VSYNDSAIYQSDINILKSNQWLNDSIISFYLEWLKDGGEDNK-NKIPNQVLLLSPSVVFC 66

Query: 66  ------DWDIFISTIESLNLHEKKLIFFVINDNSNVS-SSGGSHWSLLVYCRKRNLFMHY 125
                 + +I +   + L+L  K++IFF + +N + +   GG+HWSLL++ +  N F++Y
Sbjct: 67  CSFVESEQEIQLMFEQPLSLKTKEVIFFPLTNNRDPNVIGGGTHWSLLIFIKSLNKFIYY 126

Query: 126 DSLSTMNSEPAFTLYESVSGYINYDPAE------FMECRAPRQNNGYDCGLYVM 165
           DS+++ NS  A  +       ++  P +       +  + P+Q NGYDCGLYV+
Sbjct: 127 DSINSFNSSDAIFIISKFKFLLSSPPPKTNLKEFLINQKTPQQQNGYDCGLYVL 179

[Close]
BLAST of Carg07216 vs. TrEMBL
Match: tr|A0A151TN41|A0A151TN41_CAJCA (Sentrin-specific protease 8 OS=Cajanus cajan OX=3821 GN=KK1_022089 PE=4 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 2.5e-38
Identity = 97/219 (44.29%), Postives = 135/219 (61.64%), Query Frame = 0 

Query: 4   KFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTL 63
           K L+Y DVV+R SDL  L G  YLND II+FYF+YLS+ + S + L +P S+++ +    
Sbjct: 9   KILSYNDVVLRRSDLDILSGPYYLNDRIIEFYFSYLSSCHPSQDTLLVPPSIAFWILKCP 68

Query: 64  DWDIFISTIESLNLHEKKLIFFVINDNSNVS-SSGGSHWSLLVYCRKRNLFMHYDSLSTM 123
             +     IE L+L +K L+ F INDN +V  + GGSHWSLL Y R  NLF+H+DS  +M
Sbjct: 69  VAEALSDFIEPLHLSDKTLVIFPINDNDDVERAEGGSHWSLLAYYRNANLFVHHDSCRSM 128

Query: 124 NSEPAFTLYESVSGYI----NYDPAEFMECR-APRQNNGYDCGLYVMETARVIYEHYFNV 183
           N+ PA  LY++VSGY+    +   A FMEC  +P Q NGYDCGLYV   AR+I   + N 
Sbjct: 129 NAAPAKQLYKAVSGYMGLSSSASEASFMECTDSPLQENGYDCGLYVTAIARIICSWHVN- 188

Query: 184 DQFRSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRL 217
              ++ D      +V++   S  V ++MR E++ LIR L
Sbjct: 189 --HKNTDTNDLWFSVVKEQVSPSVVASMRGEILALIRDL 224

[Close]
BLAST of Carg07216 vs. TrEMBL
Match: tr|A0A1S3THK8|A0A1S3THK8_VIGRR (NEDD8-specific protease 1 OS=Vigna radiata var. radiata OX=3916 GN=LOC106755582 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 4.3e-38
Identity = 97/219 (44.29%), Postives = 134/219 (61.19%), Query Frame = 0 

Query: 4   KFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTL 63
           K L+Y DVV+R SDL  L G  YLND II+FYF+YLS+++ S ++L +P S+++ +    
Sbjct: 9   KILSYNDVVLRQSDLEILSGPYYLNDRIIEFYFSYLSSSHSSQDMLLVPPSIAFWIMQCP 68

Query: 64  DWDIFISTIESLNLHEKKLIFFVINDNSNVS-SSGGSHWSLLVYCRKRNLFMHYDSLSTM 123
             D     +E L+L +K+L+ F INDN +V  + GGSHWSL+ YCR  N+F+H+DS   M
Sbjct: 69  VVDSLGDFLEPLHLPDKELVIFPINDNDDVDRAEGGSHWSLVAYCRNANVFVHHDSCRGM 128

Query: 124 NSEPAFTLYESVSGYI----NYDPAEFMEC-RAPRQNNGYDCGLYVMETARVIYEHYFNV 183
           N+ PA  LY++V+GY+    +   A F EC  +P Q+NGYDCGLYV  TAR I   + N 
Sbjct: 129 NAAPAKKLYKAVAGYMGSSGSATEASFQECTNSPLQDNGYDCGLYVTATARTICNWHANH 188

Query: 184 DQFRSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRL 217
           +     D       V   V S  V S MR E++ LIR L
Sbjct: 189 NNTHGNDMWFSA--VKEQVTSSAVAS-MRGEILALIRDL 224

[Close]
BLAST of Carg07216 vs. TrEMBL
Match: tr|A0A2G5D630|A0A2G5D630_AQUCA (Uncharacterized protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_02700117v1 PE=4 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 1.3e-37
Identity = 95/219 (43.38%), Postives = 140/219 (63.93%), Query Frame = 0 

Query: 4   KFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTL 63
           K L+Y DVV+R SDL  L G ++LND II+FYF+YL++ Y S +IL +P S+S+ + N  
Sbjct: 9   KILSYNDVVLRRSDLDILSGPHFLNDRIIEFYFSYLASCYASEDILLVPPSISFWISNCP 68

Query: 64  DWDIFISTIESLNLHEKKLIFFVINDNSNVS-SSGGSHWSLLVYCRKRNLFMHYDSLSTM 123
           D +     +E L   +KK++ F +N+N +VS + GGSHWSLL Y RK+N+F+H+DSL++ 
Sbjct: 69  DIESLKDFVEPLKFPDKKMVIFPVNNNDDVSMAEGGSHWSLLAYERKKNVFVHHDSLASS 128

Query: 124 NSEPAFTLYESVSGYINYD----PAEFMEC-RAPRQNNGYDCGLYVMETARVIYEHYFNV 183
           N   A  LY+ V  ++        A F++C  +P+Q NGYDCGLYV   A+VI E Y N 
Sbjct: 129 NRCHAKRLYKGVVKFMGISDSKVDAPFIDCTTSPQQMNGYDCGLYVTAIAKVICEWYVN- 188

Query: 184 DQFRSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRL 217
              RS+D  S   ++   + +  V SNMR+E++ LI+ L
Sbjct: 189 --GRSEDMDSWFTSLKTQITTSSV-SNMRNEILLLIKGL 223

[Close]
BLAST of Carg07216 vs. TrEMBL
Match: tr|C6TMM4|C6TMM4_SOYBN (Uncharacterized protein OS=Glycine max OX=3847 GN=100800268 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 3.7e-37
Identity = 92/219 (42.01%), Postives = 134/219 (61.19%), Query Frame = 0 

Query: 4   KFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTL 63
           K L+Y DVV+R SDLH L G  +LND II+FYF+YLS+ + S +IL +P S+++ +    
Sbjct: 9   KILSYNDVVLRRSDLHILSGPYFLNDRIIEFYFSYLSSCHPSQDILLVPPSIAFWIIQCP 68

Query: 64  DWDIFISTIESLNLHEKKLIFFVINDNSNVS-SSGGSHWSLLVYCRKRNLFMHYDSLSTM 123
             +     IE L+L +K L+ F +NDN +V  + GGSHWSLL Y R  NLF+H+DS  +M
Sbjct: 69  VAEALGDFIEPLHLPDKTLVIFPVNDNDDVDRAEGGSHWSLLAYYRNANLFVHHDSCRSM 128

Query: 124 NSEPAFTLYESVSGYI----NYDPAEFMEC-RAPRQNNGYDCGLYVMETARVIYEHYFNV 183
           N+ PA  LY+ ++GY+    +   A F+EC  +P Q+NGYDCGLY    ARVI   + N 
Sbjct: 129 NAAPAKQLYKGIAGYMGSSGSASEASFLECTNSPMQDNGYDCGLYATAIARVICNWHVN- 188

Query: 184 DQFRSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRL 217
              ++ D      + ++   S    ++MR E++ LIR L
Sbjct: 189 --HKNADTNDLWFSAVKEQVSPSAVASMRGEILALIRDL 224

[Close]
BLAST of Carg07216 vs. TrEMBL
Match: tr|A0A0B2P8N4|A0A0B2P8N4_GLYSO (NEDD8-specific protease 1 OS=Glycine soja OX=3848 GN=glysoja_024676 PE=4 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 3.7e-37
Identity = 92/219 (42.01%), Postives = 134/219 (61.19%), Query Frame = 0 

Query: 4   KFLTYGDVVIRGSDLHSLEGRNYLNDTIIDFYFAYLSATYQSNEILSIPTSVSYLLGNTL 63
           K L+Y DVV+R SDLH L G  +LND II+FYF+YLS+ + S +IL +P S+++ +    
Sbjct: 9   KILSYNDVVLRRSDLHILSGPYFLNDRIIEFYFSYLSSCHPSQDILLVPPSIAFWIIQCP 68

Query: 64  DWDIFISTIESLNLHEKKLIFFVINDNSNVS-SSGGSHWSLLVYCRKRNLFMHYDSLSTM 123
             +     IE L+L +K L+ F +NDN +V  + GGSHWSLL Y R  NLF+H+DS  +M
Sbjct: 69  VAEALGDFIEPLHLPDKTLVIFPVNDNDDVDRAEGGSHWSLLAYYRNANLFVHHDSCRSM 128

Query: 124 NSEPAFTLYESVSGYI----NYDPAEFMEC-RAPRQNNGYDCGLYVMETARVIYEHYFNV 183
           N+ PA  LY+ ++GY+    +   A F+EC  +P Q+NGYDCGLY    ARVI   + N 
Sbjct: 129 NAAPAKQLYKGIAGYMGSSGSASEASFLECTNSPMQDNGYDCGLYATAIARVICNWHVN- 188

Query: 184 DQFRSKDEISGTINVMRSVKSKYVESNMRSEMMQLIRRL 217
              ++ D      + ++   S    ++MR E++ LIR L
Sbjct: 189 --HKNADTNDLWFSAVKEQVSPSAVASMRGEILALIRDL 224

The following BLAST results are available for this feature:
BLAST of Carg07216 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022953908.11.7e-11897.73NEDD8-specific protease 1-like [Cucurbita moschata][more]
XP_023549404.11.6e-11393.69NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo][more]
XP_022988745.17.6e-8772.94NEDD8-specific protease 1-like [Cucurbita maxima][more]
XP_022942008.12.7e-8472.17NEDD8-specific protease 1-like [Cucurbita moschata][more]
XP_023526001.19.1e-6473.75NEDD8-specific protease 1-like [Cucurbita pepo subsp. pepo][more]
BLAST of Carg07216 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G60190.12.1e-3838.91Cysteine proteinases superfamily protein[more]
AT4G33620.12.1e-0622.66Cysteine proteinases superfamily protein[more]
AT1G09730.16.8e-0524.08Cysteine proteinases superfamily protein[more]
BLAST of Carg07216 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9LSS7|RUBP1_ARATH3.8e-3738.91NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1[more]
sp|Q5FVJ8|SENP8_RAT1.1e-2337.14Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1[more]
sp|Q96LD8|SENP8_HUMAN1.8e-2336.57Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1[more]
sp|Q9D2Z4|SENP8_MOUSE1.5e-2236.57Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2[more]
sp|Q54XR2|SENP8_DICDI6.7e-1831.03Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=sen... [more]
BLAST of Carg07216 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A151TN41|A0A151TN41_CAJCA2.5e-3844.29Sentrin-specific protease 8 OS=Cajanus cajan OX=3821 GN=KK1_022089 PE=4 SV=1[more]
tr|A0A1S3THK8|A0A1S3THK8_VIGRR4.3e-3844.29NEDD8-specific protease 1 OS=Vigna radiata var. radiata OX=3916 GN=LOC106755582 ... [more]
tr|A0A2G5D630|A0A2G5D630_AQUCA1.3e-3743.38Uncharacterized protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_02700117v1 PE=4... [more]
tr|C6TMM4|C6TMM4_SOYBN3.7e-3742.01Uncharacterized protein OS=Glycine max OX=3847 GN=100800268 PE=2 SV=1[more]
tr|A0A0B2P8N4|A0A0B2P8N4_GLYSO3.7e-3742.01NEDD8-specific protease 1 OS=Glycine soja OX=3848 GN=glysoja_024676 PE=4 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0008234cysteine-type peptidase activity
Vocabulary: INTERPRO
TermDefinition
IPR038765Papain_like_cys_pep_sf
IPR003653Peptidase_C48_C
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0008234 cysteine-type peptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07216-RACarg07216-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003653Ulp1 protease family, C-terminal catalytic domainPFAMPF02902Peptidase_C48coord: 76..172
e-value: 8.1E-12
score: 45.3
IPR003653Ulp1 protease family, C-terminal catalytic domainPROSITEPS50600ULP_PROTEASEcoord: 11..170
score: 16.498
NoneNo IPR availableGENE3DG3DSA:3.40.395.10coord: 2..219
e-value: 3.0E-55
score: 189.1
NoneNo IPR availablePANTHERPTHR12606SENTRIN/SUMO-SPECIFIC PROTEASEcoord: 8..194
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILYSSF54001Cysteine proteinasescoord: 4..217