Carg06471 (gene) Silver-seed gourd

NameCarg06471
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionVHS domain-containing protein At3g16270
LocationCucurbita_argyrosperma_scaffold_051 : 715174 .. 718595 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGATGCGGCGACTTCGGACGAGGACAAGGTCACCCCGGTGTACAAATTGGAAGAGATTTGCGAAGTTTTGAGATCTTCGCATGTCAGTATTGTCAAGGAGTTTTCGGAATTCATTCTGAAGAGGCTTGAACATAAGAGCCCGATTGTCAAACAGAAGGTATCTACATCTTTTCTGCTCGTTCTGTTCTGATTCCTCTTATTTTAATTTCGAACTTAATTTGGTGGATGTGTTATCCAGGGAGTAGTATATTTTATCAGTGCAATTAGGACTTTTGTTCTCTGATTCTTCGGTGGTCTTAGAAAGTGCGGCAGAGTCAAGTAATTCGTTTTTGGGTTGTGTTGTAAGCTTCCTAATTCGGCGAAATGTTTAGTTTTTATGCTAGCTCGGGAATCGCTTGGTTTTGGTATTTCCACATTGTTCAAATGTTGATAGAATAATGAACACAAAAATTCATACATATCCCTTTCAGTTTTCTTTTACTTATGTTTTCTCAGAGATGATAATTTTAGAGCTTATAATCTGAAGTATTTTTTATATAATTGATATTGACTTAGCTTGTTTTGTTTTAAATCTTGTCTCTTCCATGATATTCGTCGTTATAGACTGGGCGACATGAATATAATGTAGAGTTGTATCATTTGGTTGATAGCTATTATATACTTTTAATTTAGTTTTTGTATTCTGCGGATGGCTAGTTTGATACTTAACGCTCGCCGCAGCTTAATTAAAATTCCATGAGTTGAAATTCTCCCATTGAAAGTTTCCATGTGTTTTTCTTATTTGTGAAAATTTGAACTCGTGCTTGATTTTACTACAGGCTCTTAGGTTGATAAAGTATGCAGTTGGGAAGTCTGGTGTGGAATTCAGAAGAGAAATGCAGAGGCACTCTGTGGCTGTCCGCCAGTTATTTCATTACAAGGGACAACCGGACCCCCTTAAAGGTGATGCACTCAATAAAGCTGTGAGGGAGACTGCTCATGATGCCATTTCTGCTATCTTTGCTGAAGAGGATAACAAGCCTGCACCATCTGAGAATCTTAACCGTCGAATTCAAGGTTTTGGGAATTCAAATTATGAACCGCCACCAGAAGATAAAAAATCATTTCTTAGCGAGGTAGTTGGTCTAGGAAGTGCATCAATCAAACAAGGTCTAAGTAATTTTGCACAAGGTCATTCATCAAGAAAGAATGGCACTAGTAGCCCTCGGGGTCCCAATCTTCAGAGGTCGTTGACTACTGAAATTGAGTATGATAATAGATATGAACCAGTTGAATATGGTCGTGAGACTCTGGGGACATCAAAGAGTACTACTAGTGGAACTTGGAACCAGGATTCTAGGGTAAGTAATGGGAACTCAAGTTCTGGCTCTTCAGTGAGCAAAACTCGAGAAGAGAGGTTACTGGAGACCATTGCAACAGCAGGTGGTGTGCGTTTGCAACCAACTCGAGATGCCATTCAAGCATTTCTTGTGGAAGCTGCAAAGTTAGATGCATTGGTGCTGAGTCATGCTCTCGAAACAAAGCTTAAATCCCCATCATGGCAGGCATGTTCTTGCTGTTAATAATCTTTTCTATGATATTATATGAGCAAAGGCTGGCTTTATGTTTCGGATTTAAAAAATGATAGTTGATATTTGTATGTCGATTTGTCACTCAATCTGATTGATGAGAATTTCACTCTTCAGGTTCGCTTCAAAGCTCTCTGCATTCTTGAGTCAATTGTTAGGAGAAGTGGCGATGAACATTTTTCAATTGTGACATCTTATTTCAGTGAAAATCAAGATGCAGTAATTGGATGTTCTGAATCCCCCCAGGCATCTCTTAGAGACAAAGCTAGCAAGGTAAATCATCTGTCTGCCTGCAAAACATAATGTTTATGCTTGTTTTCTCGTTTTCTTTGAGTAACTTTCTTCTTAAAGTGGTATTGGAGGCATGTTTTTTTTTCCTTCCTGATGGGGAAAAGATCATATCAGAATTAACTCCTCCATCTTACTTAATCCTGCCGAGATGCAAAAGTTATTAGAATATCATAATTACTACGAGTTGTAGGTACTTGTTGACATTGTTATATCCATGAGCCTTTTTGTAATCACTTTTTCTGTTACGATGTCTTACTTTTCATCTATAAGATTTGGTTCACTAAGGAAGCTATTACTTTGCTTGACTAAGGAAATATTTCCTTGAATACTATAGAACCTGTTATAATGCTATTACTTTTCTTCTCCTTTGAAATGAATATTTACTCTTTAAAAATTGAAGGAAGCCTCTGCATGATAGTTGATAAAATTGTTGAATAAATCCGTCATTTGGATTTTTATCCTTTGATACAGTATAATTTGGTTACAATTGCTTGAGAAAATAATATAGGCTCAACTTTGGGGTCTTATCCTGAAAAGGAATGATAATTTATTCCCTTGTCTCTTTTGGCAGGTTATGCCTCTTTTAGATGGAGGAAAAGGAGTTCCCCCCATGAATGATTCCGAGAAGTCTCTACCAAGCAACACCAGTTCCACCATTCAGATGCCAGACTTATTAGACACTAGTGATGCAGGTGATTACGGTGGAACAGACAAATCCTTAGAGGTGGAAAACCTGTCGAGTGTCCCATTAGTAGATGACTTATTTGGAGGTGGCCTAAACACCGTCACAAGCACCAGCGAACTAAAGAATGACGACGACCCCTTTTCAGATGTCCTGTTTCATACAACTGAAACTAGAGAAAATCCAGATGATCTATTTTCTGGGATGAACTTTGAAAATAATCAGGTTACTGATGAAAATAAAGAGCCTACCTCAGAACAGAAAAATGAACCTGGAGTTTTTGATATTTTCGGATCAAGTTCTGAACCTGCTGTACAAGAACATGCAAGGAAAGATGTTATTGATTTGATGAGTGGTTTGTCCATTCATGAAGATGCATTGAAGAACAAAGATAAAGGAGATTCTAAGGATTCACTGTCTGAATCTTTATTCTCGGTTTCTAGTCAACCCAACCATCAGAATCAGGTTCCTCATGATTCTTTAACTGGCACGTACAGTTCACCAATGGTTGGGACAAATATGAATGCCACCTTTTTCCCTGGAATGCCTTATCTTCCCTCAGGCATGATGTTCAATCCAGCCTTTTCTTCTCAGCCAATGGGTTATGCTCCCACCGGAAACTTCTTTACTCAACAACAATTATTATCAGCCATGTCCAATTACCAACAGTTTGGGAACCCCAATCTCCAATCAAGCGGTGGAGGATATTCTTCACCCCTTCCGGACATATTCCAGCCAAATCTTGCAGCACAATCACCTAGTTCTGTGATGAATAGTTCAAAGAAGGAAGATACCAGAGCTTTTGATTTTATCTCGGTAAGTTTAATTTCATCCAACTTTTTGATAATTTTCGTAACTTTATGCCCTGAATTTTAA

mRNA sequence

ATGATTGATGCGGCGACTTCGGACGAGGACAAGGTCACCCCGGTGTACAAATTGGAAGAGATTTGCGAAGTTTTGAGATCTTCGCATGTCAGTATTGTCAAGGAGTTTTCGGAATTCATTCTGAAGAGGCTTGAACATAAGAGCCCGATTGTCAAACAGAAGGCTCTTAGGTTGATAAAGTATGCAGTTGGGAAGTCTGGTGTGGAATTCAGAAGAGAAATGCAGAGGCACTCTGTGGCTGTCCGCCAGTTATTTCATTACAAGGGACAACCGGACCCCCTTAAAGGTGATGCACTCAATAAAGCTGTGAGGGAGACTGCTCATGATGCCATTTCTGCTATCTTTGCTGAAGAGGATAACAAGCCTGCACCATCTGAGAATCTTAACCGTCGAATTCAAGGTTTTGGGAATTCAAATTATGAACCGCCACCAGAAGATAAAAAATCATTTCTTAGCGAGGTAGTTGGTCTAGGAAGTGCATCAATCAAACAAGGTCTAAGTAATTTTGCACAAGGTCATTCATCAAGAAAGAATGGCACTAGTAGCCCTCGGGGTCCCAATCTTCAGAGGTCGTTGACTACTGAAATTGAGTATGATAATAGATATGAACCAGTTGAATATGGTCGTGAGACTCTGGGGACATCAAAGAGTACTACTAGTGGAACTTGGAACCAGGATTCTAGGGTAAGTAATGGGAACTCAAGTTCTGGCTCTTCAGTGAGCAAAACTCGAGAAGAGAGGTTACTGGAGACCATTGCAACAGCAGGTGGTGTGCGTTTGCAACCAACTCGAGATGCCATTCAAGCATTTCTTGTGGAAGCTGCAAATGAAAATCAAGATGCAGTAATTGGATGTTCTGAATCCCCCCAGGCATCTCTTAGAGACAAAGCTAGCAAGGTTATGCCTCTTTTAGATGGAGGAAAAGGAGTTCCCCCCATGAATGATTCCGAGAAGTCTCTACCAAGCAACACCAGTTCCACCATTCAGATGCCAGACTTATTAGACACTAGTGATGCAGGTGATTACGGTGGAACAGACAAATCCTTAGAGGTGGAAAACCTGTCGAGTGTCCCATTAGTAGATGACTTATTTGGAGGTGGCCTAAACACCGTCACAAGCACCAGCGAACTAAAGAATGACGACGACCCCTTTTCAGATGTCCTGTTTCATACAACTGAAACTAGAGAAAATCCAGATGATCTATTTTCTGGGATGAACTTTGAAAATAATCAGGTTACTGATGAAAATAAAGAGCCTACCTCAGAACAGAAAAATGAACCTGGAGTTTTTGATATTTTCGGATCAAGTTCTGAACCTGCTGTACAAGAACATGCAAGGAAAGATGTTATTGATTTGATGAGTGGTTTGTCCATTCATGAAGATGCATTGAAGAACAAAGATAAAGGAGATTCTAAGGATTCACTGTCTGAATCTTTATTCTCGGTTTCTAGTCAACCCAACCATCAGAATCAGGTTCCTCATGATTCTTTAACTGGCACGTACAGTTCACCAATGGTTGGGACAAATATGAATGCCACCTTTTTCCCTGGAATGCCTTATCTTCCCTCAGGCATGATGTTCAATCCAGCCTTTTCTTCTCAGCCAATGGGTTATGCTCCCACCGGAAACTTCTTTACTCAACAACAATTATTATCAGCCATGTCCAATTACCAACAGTTTGGGAACCCCAATCTCCAATCAAGCGGTGGAGGATATTCTTCACCCCTTCCGGACATATTCCAGCCAAATCTTGCAGCACAATCACCTAGTTCTGTGATGAATAGTTCAAAGAAGGAAGATACCAGAGCTTTTGATTTTATCTCGGTAAGTTTAATTTCATCCAACTTTTTGATAATTTTCGTAACTTTATGCCCTGAATTTTAA

Coding sequence (CDS)

ATGATTGATGCGGCGACTTCGGACGAGGACAAGGTCACCCCGGTGTACAAATTGGAAGAGATTTGCGAAGTTTTGAGATCTTCGCATGTCAGTATTGTCAAGGAGTTTTCGGAATTCATTCTGAAGAGGCTTGAACATAAGAGCCCGATTGTCAAACAGAAGGCTCTTAGGTTGATAAAGTATGCAGTTGGGAAGTCTGGTGTGGAATTCAGAAGAGAAATGCAGAGGCACTCTGTGGCTGTCCGCCAGTTATTTCATTACAAGGGACAACCGGACCCCCTTAAAGGTGATGCACTCAATAAAGCTGTGAGGGAGACTGCTCATGATGCCATTTCTGCTATCTTTGCTGAAGAGGATAACAAGCCTGCACCATCTGAGAATCTTAACCGTCGAATTCAAGGTTTTGGGAATTCAAATTATGAACCGCCACCAGAAGATAAAAAATCATTTCTTAGCGAGGTAGTTGGTCTAGGAAGTGCATCAATCAAACAAGGTCTAAGTAATTTTGCACAAGGTCATTCATCAAGAAAGAATGGCACTAGTAGCCCTCGGGGTCCCAATCTTCAGAGGTCGTTGACTACTGAAATTGAGTATGATAATAGATATGAACCAGTTGAATATGGTCGTGAGACTCTGGGGACATCAAAGAGTACTACTAGTGGAACTTGGAACCAGGATTCTAGGGTAAGTAATGGGAACTCAAGTTCTGGCTCTTCAGTGAGCAAAACTCGAGAAGAGAGGTTACTGGAGACCATTGCAACAGCAGGTGGTGTGCGTTTGCAACCAACTCGAGATGCCATTCAAGCATTTCTTGTGGAAGCTGCAAATGAAAATCAAGATGCAGTAATTGGATGTTCTGAATCCCCCCAGGCATCTCTTAGAGACAAAGCTAGCAAGGTTATGCCTCTTTTAGATGGAGGAAAAGGAGTTCCCCCCATGAATGATTCCGAGAAGTCTCTACCAAGCAACACCAGTTCCACCATTCAGATGCCAGACTTATTAGACACTAGTGATGCAGGTGATTACGGTGGAACAGACAAATCCTTAGAGGTGGAAAACCTGTCGAGTGTCCCATTAGTAGATGACTTATTTGGAGGTGGCCTAAACACCGTCACAAGCACCAGCGAACTAAAGAATGACGACGACCCCTTTTCAGATGTCCTGTTTCATACAACTGAAACTAGAGAAAATCCAGATGATCTATTTTCTGGGATGAACTTTGAAAATAATCAGGTTACTGATGAAAATAAAGAGCCTACCTCAGAACAGAAAAATGAACCTGGAGTTTTTGATATTTTCGGATCAAGTTCTGAACCTGCTGTACAAGAACATGCAAGGAAAGATGTTATTGATTTGATGAGTGGTTTGTCCATTCATGAAGATGCATTGAAGAACAAAGATAAAGGAGATTCTAAGGATTCACTGTCTGAATCTTTATTCTCGGTTTCTAGTCAACCCAACCATCAGAATCAGGTTCCTCATGATTCTTTAACTGGCACGTACAGTTCACCAATGGTTGGGACAAATATGAATGCCACCTTTTTCCCTGGAATGCCTTATCTTCCCTCAGGCATGATGTTCAATCCAGCCTTTTCTTCTCAGCCAATGGGTTATGCTCCCACCGGAAACTTCTTTACTCAACAACAATTATTATCAGCCATGTCCAATTACCAACAGTTTGGGAACCCCAATCTCCAATCAAGCGGTGGAGGATATTCTTCACCCCTTCCGGACATATTCCAGCCAAATCTTGCAGCACAATCACCTAGTTCTGTGATGAATAGTTCAAAGAAGGAAGATACCAGAGCTTTTGATTTTATCTCGGTAAGTTTAATTTCATCCAACTTTTTGATAATTTTCGTAACTTTATGCCCTGAATTTTAA

Protein sequence

MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDNKPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGTSSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNGNSSSGSSVSKTREERLLETIATAGGVRLQPTRDAIQAFLVEAANENQDAVIGCSESPQASLRDKASKVMPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFGGGLNTVTSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDENKEPTSEQKNEPGVFDIFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQPNHQNQVPHDSLTGTYSSPMVGTNMNATFFPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFTQQQLLSAMSNYQQFGNPNLQSSGGGYSSPLPDIFQPNLAAQSPSSVMNSSKKEDTRAFDFISVSLISSNFLIIFVTLCPEF
BLAST of Carg06471 vs. NCBI nr
Match: XP_022979433.1 (VHS domain-containing protein At3g16270-like [Cucurbita maxima])

HSP 1 Score: 1073.5 bits (2775), Expect = 1.9e-310
Identity = 604/657 (91.93%), Postives = 607/657 (92.39%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 75

Query: 61  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN 120
           YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN
Sbjct: 76  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN 135

Query: 121 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 180
           KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT
Sbjct: 136 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 195

Query: 181 SSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNGNSSSGSSV 240
           SSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKST SGTWNQDSRVSNGNSSSGSSV
Sbjct: 196 SSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTISGTWNQDSRVSNGNSSSGSSV 255

Query: 241 SKTREERLLETIATAGGVRLQPTRDAIQAFLVEAA------------------------- 300
           SKTREERLLETIATAGGVRLQPTRDAIQAFLVEAA                         
Sbjct: 256 SKTREERLLETIATAGGVRLQPTRDAIQAFLVEAAMLDALALSNALETKLKSPSWQVRFK 315

Query: 301 ------------------------NENQDAVIGCSESPQASLRDKASKVMXXXXXXXXXX 360
                                   +ENQDAVIGCSESPQASLRDKASKVMXXXXXXXXXX
Sbjct: 316 ALCILESIVRRSGDEHFSIVTSYFSENQDAVIGCSESPQASLRDKASKVMXXXXXXXXXX 375

Query: 361 XXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFGGGLNTV 420
           XXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFGGGLNTV
Sbjct: 376 XXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFGGGLNTV 435

Query: 421 TSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDENKEPTSEQKNEPGVFD 480
           TSTSELKNDDDPFSDVLFHTTETRENPDD+FSGMNFENNQVTDENK+PTSEQKNEPGVFD
Sbjct: 436 TSTSELKNDDDPFSDVLFHTTETRENPDDIFSGMNFENNQVTDENKKPTSEQKNEPGVFD 495

Query: 481 IFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQPNHQNQ 540
           IFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQPNHQNQ
Sbjct: 496 IFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQPNHQNQ 555

Query: 541 VPHDSLTGTYSSPMVGTNMNATFFPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFTQQQLL 600
           VPHDSLTGTYSSPMVGTNMNATFFPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFTQQQLL
Sbjct: 556 VPHDSLTGTYSSPMVGTNMNATFFPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFTQQQLL 615

Query: 601 SAMSNYQQFGNPNLQSSGGGYSSPLPDIFQPNLAAQSPSSVMNSSKKEDTRAFDFIS 609
           SAMSNYQQFGNPNLQSSGGGYSSPLPDIFQPNLAAQSPSSVMNSSKKEDTRAFDFIS
Sbjct: 616 SAMSNYQQFGNPNLQSSGGGYSSPLPDIFQPNLAAQSPSSVMNSSKKEDTRAFDFIS 672

BLAST of Carg06471 vs. NCBI nr
Match: XP_023526954.1 (VHS domain-containing protein At3g16270-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1072.4 bits (2772), Expect = 5.7e-310
Identity = 604/657 (91.93%), Postives = 607/657 (92.39%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 75

Query: 61  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN 120
           YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN
Sbjct: 76  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN 135

Query: 121 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 180
           KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT
Sbjct: 136 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 195

Query: 181 SSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNGNSSSGSSV 240
           SSPRGPNLQRSLTTEI YDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNGNSSSGSSV
Sbjct: 196 SSPRGPNLQRSLTTEIAYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNGNSSSGSSV 255

Query: 241 SKTREERLLETIATAGGVRLQPTRDAIQAFLVEAA------------------------- 300
           SKTREERLLETIATAGGVRLQPTRDAIQAFLVEAA                         
Sbjct: 256 SKTREERLLETIATAGGVRLQPTRDAIQAFLVEAAKLDALVLSHALETKLKSPSWQVRFK 315

Query: 301 ------------------------NENQDAVIGCSESPQASLRDKASKVMXXXXXXXXXX 360
                                   +ENQDAVIGCSESPQASLRDKASKVMXXXXXXXXXX
Sbjct: 316 ALCILESIVRRSGDEHFSIVTSYFSENQDAVIGCSESPQASLRDKASKVMXXXXXXXXXX 375

Query: 361 XXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFGGGLNTV 420
           XXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFGGGLNTV
Sbjct: 376 XXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFGGGLNTV 435

Query: 421 TSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDENKEPTSEQKNEPGVFD 480
           TSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFEN+QVTDENK+PTSEQKNEPGVFD
Sbjct: 436 TSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENDQVTDENKKPTSEQKNEPGVFD 495

Query: 481 IFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQPNHQNQ 540
           IFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQPNHQNQ
Sbjct: 496 IFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQPNHQNQ 555

Query: 541 VPHDSLTGTYSSPMVGTNMNATFFPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFTQQQLL 600
           VPHDSLTGTYSSPMVGTNMNATFFPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFTQQQLL
Sbjct: 556 VPHDSLTGTYSSPMVGTNMNATFFPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFTQQQLL 615

Query: 601 SAMSNYQQFGNPNLQSSGGGYSSPLPDIFQPNLAAQSPSSVMNSSKKEDTRAFDFIS 609
           SAMSNYQQFGNPNLQSSGGGYSSPLPDIFQPNLAAQSPSSVMNSSKKEDTRAFDFIS
Sbjct: 616 SAMSNYQQFGNPNLQSSGGGYSSPLPDIFQPNLAAQSPSSVMNSSKKEDTRAFDFIS 672

BLAST of Carg06471 vs. NCBI nr
Match: XP_022955654.1 (VHS domain-containing protein At3g16270-like [Cucurbita moschata])

HSP 1 Score: 1068.9 bits (2763), Expect = 6.4e-309
Identity = 602/657 (91.63%), Postives = 606/657 (92.24%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 75

Query: 61  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN 120
           YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN
Sbjct: 76  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN 135

Query: 121 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 180
           KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIK GLSNFAQGHSSRKNGT
Sbjct: 136 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKHGLSNFAQGHSSRKNGT 195

Query: 181 SSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNGNSSSGSSV 240
           SSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNGNSSSGSSV
Sbjct: 196 SSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNGNSSSGSSV 255

Query: 241 SKTREERLLETIATAGGVRLQPTRDAIQAFLVEAA------------------------- 300
           SKTREERLLETIATAGGVRLQPTRDAIQAFLVEAA                         
Sbjct: 256 SKTREERLLETIATAGGVRLQPTRDAIQAFLVEAAKLDALVLSHALETKLKSPSWQVRFK 315

Query: 301 ------------------------NENQDAVIGCSESPQASLRDKASKVMXXXXXXXXXX 360
                                   +ENQDAVIGCSESPQASLRDKASKVMXXXXXXXXXX
Sbjct: 316 ALCILESIVRRSGDEHFSIVTSYFSENQDAVIGCSESPQASLRDKASKVMXXXXXXXXXX 375

Query: 361 XXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFGGGLNTV 420
           XXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFG GLNTV
Sbjct: 376 XXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFGVGLNTV 435

Query: 421 TSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDENKEPTSEQKNEPGVFD 480
           TSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDENK+PTSEQKNEPGVFD
Sbjct: 436 TSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDENKKPTSEQKNEPGVFD 495

Query: 481 IFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQPNHQNQ 540
           IFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQPNHQNQ
Sbjct: 496 IFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQPNHQNQ 555

Query: 541 VPHDSLTGTYSSPMVGTNMNATFFPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFTQQQLL 600
           VPHDSLTGTYSSPMVGTNMNAT+FPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFTQQQLL
Sbjct: 556 VPHDSLTGTYSSPMVGTNMNATYFPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFTQQQLL 615

Query: 601 SAMSNYQQFGNPNLQSSGGGYSSPLPDIFQPNLAAQSPSSVMNSSKKEDTRAFDFIS 609
           SAMSNYQQFGNPNLQSSGGGYSSPLPDIFQPNLAAQSPSS+MNSSKKEDTRAFDFIS
Sbjct: 616 SAMSNYQQFGNPNLQSSGGGYSSPLPDIFQPNLAAQSPSSMMNSSKKEDTRAFDFIS 672

BLAST of Carg06471 vs. NCBI nr
Match: XP_008447533.1 (PREDICTED: VHS domain-containing protein At3g16270 [Cucumis melo] >XP_008447534.1 PREDICTED: VHS domain-containing protein At3g16270 [Cucumis melo] >XP_016900415.1 PREDICTED: VHS domain-containing protein At3g16270 [Cucumis melo])

HSP 1 Score: 968.0 bits (2501), Expect = 1.5e-278
Identity = 523/663 (78.88%), Postives = 549/663 (82.81%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSP+VKQKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPVVKQKALRLIK 75

Query: 61  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN 120
           YAVGKSGVEFRREMQR+SVAVRQLFHYKGQPDPLKGDALNKAVR+TAH+AIS+IFAEEDN
Sbjct: 76  YAVGKSGVEFRREMQRNSVAVRQLFHYKGQPDPLKGDALNKAVRDTAHEAISSIFAEEDN 135

Query: 121 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 180
           KPAPSENLNRRIQGFGNSNYEPP EDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT
Sbjct: 136 KPAPSENLNRRIQGFGNSNYEPPSEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 195

Query: 181 SSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNGNSSSGSSV 240
           SS RG NLQRSLTTE+EYDNRYEPVEYGRETLGT++STTSGTWNQDSRVSNG+ SSGSS 
Sbjct: 196 SSHRGINLQRSLTTEMEYDNRYEPVEYGRETLGTARSTTSGTWNQDSRVSNGSPSSGSSE 255

Query: 241 SKTREERLLETIATAGGVRLQPTRDAIQAFLVEAA------------------------- 300
           SKTRE+RLL+TIATAGGVRLQPTRD+IQAFLVEA                          
Sbjct: 256 SKTREDRLLDTIATAGGVRLQPTRDSIQAFLVEAVKLDALALSNALETKLKSPSWQVRFK 315

Query: 301 ------------------------NENQDAVIGCSESPQASLRDKASKVMXXXXXXXXXX 360
                                   +ENQ+AVIGCSESPQASLR+KASKVM          
Sbjct: 316 ALCILESIVRRNDDDHFSIVTSYFSENQEAVIGCSESPQASLREKASKVMPLLDGGKGVP 375

Query: 361 XXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFGGGLNTV 420
                               DL+DTSDAGDY GT+KS+EVENLSS PLVDDLFG GLNTV
Sbjct: 376 SMNVSEKSLPSNTSSTIQMPDLIDTSDAGDYSGTNKSVEVENLSSTPLVDDLFGDGLNTV 435

Query: 421 TSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDENKEPTSEQKNEPGVFD 480
           TSTSELKNDDDPFSDV FHT ETRENPDDLFSGMNF+NNQV++ENK+P  EQKNEPGVFD
Sbjct: 436 TSTSELKNDDDPFSDVSFHTIETRENPDDLFSGMNFDNNQVSNENKKPALEQKNEPGVFD 495

Query: 481 IFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQPNHQNQ 540
           IFGSSSEPAVQEHARKDV DLMSGLSIHED LK+KDKGDSKDSLSESLFS S QPNHQN 
Sbjct: 496 IFGSSSEPAVQEHARKDVNDLMSGLSIHEDTLKSKDKGDSKDSLSESLFSASGQPNHQNP 555

Query: 541 VPHDSLTGTYSSPMVGTNMNATFFPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFTQQQLL 600
           V  DSL G YSSPM GTNMNA FFPGM YLPSGMMFNPAFSSQPM YA +GNFFTQQQLL
Sbjct: 556 VSQDSLNGIYSSPMAGTNMNAAFFPGMTYLPSGMMFNPAFSSQPMAYAASGNFFTQQQLL 615

Query: 601 SAMSNYQQFGNPNLQSS------GGGYSSPLPDIFQPNLAAQSPSSVMNSSKKEDTRAFD 609
           SAMSNYQQFGNPNLQS+       GGYSSPLPDIFQPNLAAQS +SVMNSSKKEDTRAFD
Sbjct: 616 SAMSNYQQFGNPNLQSNSGGGVGSGGYSSPLPDIFQPNLAAQSSTSVMNSSKKEDTRAFD 675

BLAST of Carg06471 vs. NCBI nr
Match: XP_004151053.1 (PREDICTED: VHS domain-containing protein At3g16270 [Cucumis sativus] >KGN58048.1 hypothetical protein Csa_3G462690 [Cucumis sativus])

HSP 1 Score: 963.8 bits (2490), Expect = 2.9e-277
Identity = 521/664 (78.46%), Postives = 548/664 (82.53%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSP+VKQKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPVVKQKALRLIK 75

Query: 61  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN 120
           YAVGKSGVEFRREMQR+SVAVRQLFHYKGQPDPLKGDALNKAVR+TAH+AIS+IFAEEDN
Sbjct: 76  YAVGKSGVEFRREMQRNSVAVRQLFHYKGQPDPLKGDALNKAVRDTAHEAISSIFAEEDN 135

Query: 121 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 180
           KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT
Sbjct: 136 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 195

Query: 181 SSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNGNSSSGSSV 240
           SS RG NLQRSLTTE+EYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNG+ SSGSS 
Sbjct: 196 SSHRGINLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNGSPSSGSSE 255

Query: 241 SKTREERLLETIATAGGVRLQPTRDAIQAFLVEAA------------------------- 300
           SKTRE+RLL+TIATAGGVRLQPTRD+IQAFLVEA                          
Sbjct: 256 SKTREDRLLDTIATAGGVRLQPTRDSIQAFLVEAVKLDALALSNALETKLKSPSWQVRFK 315

Query: 301 ------------------------NENQDAVIGCSESPQASLRDKASKVMXXXXXXXXXX 360
                                   +ENQ+AVIGCSESPQASLR+KA+KVM          
Sbjct: 316 ALCILESIVRRNDDDQFSIVTSYFSENQEAVIGCSESPQASLREKATKVMPLLDGGKGVP 375

Query: 361 XXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFGGGLNTV 420
                               DL+DTSDAGD+ GT+KS+EVENLSS PLVDDLFG GLNTV
Sbjct: 376 SINVYEKSLPSNTSSTIQMPDLIDTSDAGDFSGTNKSVEVENLSSTPLVDDLFGDGLNTV 435

Query: 421 TSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDENKEPTSEQKNEPGVFD 480
           TSTSELKNDDDPFSDV FHT ETRENPDDLFSGMNF+NNQV++ENK+   E KNEPGVFD
Sbjct: 436 TSTSELKNDDDPFSDVSFHTIETRENPDDLFSGMNFDNNQVSNENKKAALEPKNEPGVFD 495

Query: 481 IFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQPNHQNQ 540
           IFGSSSEPAVQEHARKDV DLMSGLSIHED LK KDKGDSKDSLSESLFS S+QPNHQNQ
Sbjct: 496 IFGSSSEPAVQEHARKDVNDLMSGLSIHEDILKGKDKGDSKDSLSESLFSASAQPNHQNQ 555

Query: 541 VPHDSLTGTYSSPMVGTNMNATFFPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFTQQQLL 600
           V  DSL G YSSPM G+NMNA FFPGM YLPSGM+FNPAFSSQPM YA TGNFFTQQQLL
Sbjct: 556 VSQDSLNGIYSSPMAGSNMNAAFFPGMTYLPSGMVFNPAFSSQPMAYAATGNFFTQQQLL 615

Query: 601 SAMSNYQQFGNPNLQSS-------GGGYSSPLPDIFQPNLAAQSPSSVMNSSKKEDTRAF 609
           SAMSNYQQFGNPNLQS+        GGYSSP PDIFQPNLAAQS +SVMNSSKKEDTRAF
Sbjct: 616 SAMSNYQQFGNPNLQSNSGGGGVGSGGYSSPFPDIFQPNLAAQSSTSVMNSSKKEDTRAF 675

BLAST of Carg06471 vs. TAIR10
Match: AT3G16270.1 (ENTH/VHS family protein)

HSP 1 Score: 540.0 bits (1390), Expect = 1.9e-153
Identity = 347/685 (50.66%), Postives = 421/685 (61.46%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDA TSDEDKV PVYKLEEIC++LRSSHVSIVKEFSEFILKRL++KSPIVKQKALRLIK
Sbjct: 16  MIDAVTSDEDKVAPVYKLEEICDLLRSSHVSIVKEFSEFILKRLDNKSPIVKQKALRLIK 75

Query: 61  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEED- 120
           YAVGKSG EFRREMQR+SVAVR LFHYKG PDPLKGDALNKAVRETAH+ ISAIF+EE+ 
Sbjct: 76  YAVGKSGSEFRREMQRNSVAVRNLFHYKGHPDPLKGDALNKAVRETAHETISAIFSEENG 135

Query: 121 NKPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRK-- 180
            KPA  E++NRRI+GFGN+N++ P  D KSFLSEVVG+GSASIKQG+SNFAQGH  +K  
Sbjct: 136 TKPAAPESINRRIEGFGNTNFQVPSNDNKSFLSEVVGIGSASIKQGISNFAQGHLPKKNE 195

Query: 181 NGTSSPRGPNLQRSLTTEIEYDNRYEPVEYGRE-TLGTSKSTTSGTWNQDSRVSNGNSSS 240
           NG+SS RGPNL RSLT E E  +RY+PV+ G++   GTSK+TT G+W   S  ++ +S+S
Sbjct: 196 NGSSSYRGPNLHRSLTMENENFSRYDPVKLGKDGNYGTSKNTTGGSWGHASGEASESSAS 255

Query: 241 GSSVSKTREERLLETIATAGGVRLQPTRDAIQAFLVEAA--------------------- 300
               SKTREE+LLETI T+GGVRLQPTRDA+  F++EAA                     
Sbjct: 256 VRVESKTREEKLLETIVTSGGVRLQPTRDALHVFILEAAKMDAVALSIALDGKLHSPMWQ 315

Query: 301 ----------------------------NENQDAVIGCSESPQASLRDKASKVMXXXXXX 360
                                       +EN DA+  C+ESPQ+SLR+KA+KV+      
Sbjct: 316 VRMKALCVLEAILRKKEDENFSIVHTYFSENLDAIQRCAESPQSSLREKANKVL-----S 375

Query: 361 XXXXXXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSV-----PLV-D 420
                                   DL D  D G            +  S      PL+ D
Sbjct: 376 LLNGGQSSGLMSSSDNTVKREAAVDLPDLIDTGXXXXXXXXXXXXDTGSTVATAGPLMDD 435

Query: 421 DLFGGGLNTVTSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDENKEPTS 480
           D FG   +   S+SE K DDDPF+DV FH  E +E+ DDLFSGM       T   K    
Sbjct: 436 DWFGDSSDIGLSSSEKKTDDDPFADVSFHPNEEKESADDLFSGM-------TVGEKSAAV 495

Query: 481 EQKNEPGVFDIFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFS 540
              + P +FD+FGS+++   +    K++ DLM   SI E+   +  KG S  +L + LF+
Sbjct: 496 GGNHVPDLFDMFGSTAKLEAEPKDAKNINDLMGSFSIDEN--NSNQKGSSSSTLPQDLFA 555

Query: 541 VSSQPNHQNQVPHDSLTGTYSSPMVGTNMNATFFPG--MPY-LPSGMMFNPAFSSQPMGY 600
           + S  +H  Q P + + G   S   G   N T  PG  MP+  P GMM NPAF+SQP+ Y
Sbjct: 556 MPSTTSH--QAPENPVGGILGSQNPGFIQN-TMLPGGVMPFNFPQGMMMNPAFASQPLNY 615

Query: 601 APTGNFFT-QQQLLSAMSNYQQFGNPNLQSSG--------GGYSSPLPDIFQPNLAAQSP 615
           A   +    QQQ L  MSN+QQFGN N Q SG        GG  S LPDIFQPN   Q+P
Sbjct: 616 AAMASLLAQQQQYLGNMSNFQQFGNLNAQGSGNVLSMGTSGGNQSALPDIFQPNFGNQAP 675

BLAST of Carg06471 vs. Swiss-Prot
Match: sp|Q9C5H4|MTV1_ARATH (Protein MODIFIED TRANSPORT TO THE VACUOLE 1 OS=Arabidopsis thaliana OX=3702 GN=MTV1 PE=1 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 3.4e-152
Identity = 347/685 (50.66%), Postives = 421/685 (61.46%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDA TSDEDKV PVYKLEEIC++LRSSHVSIVKEFSEFILKRL++KSPIVKQKALRLIK
Sbjct: 16  MIDAVTSDEDKVAPVYKLEEICDLLRSSHVSIVKEFSEFILKRLDNKSPIVKQKALRLIK 75

Query: 61  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEED- 120
           YAVGKSG EFRREMQR+SVAVR LFHYKG PDPLKGDALNKAVRETAH+ ISAIF+EE+ 
Sbjct: 76  YAVGKSGSEFRREMQRNSVAVRNLFHYKGHPDPLKGDALNKAVRETAHETISAIFSEENG 135

Query: 121 NKPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRK-- 180
            KPA  E++NRRI+GFGN+N++ P  D KSFLSEVVG+GSASIKQG+SNFAQGH  +K  
Sbjct: 136 TKPAAPESINRRIEGFGNTNFQVPSNDNKSFLSEVVGIGSASIKQGISNFAQGHLPKKNE 195

Query: 181 NGTSSPRGPNLQRSLTTEIEYDNRYEPVEYGRE-TLGTSKSTTSGTWNQDSRVSNGNSSS 240
           NG+SS RGPNL RSLT E E  +RY+PV+ G++   GTSK+TT G+W   S  ++ +S+S
Sbjct: 196 NGSSSYRGPNLHRSLTMENENFSRYDPVKLGKDGNYGTSKNTTGGSWGHASGEASESSAS 255

Query: 241 GSSVSKTREERLLETIATAGGVRLQPTRDAIQAFLVEAA--------------------- 300
               SKTREE+LLETI T+GGVRLQPTRDA+  F++EAA                     
Sbjct: 256 VRVESKTREEKLLETIVTSGGVRLQPTRDALHVFILEAAKMDAVALSIALDGKLHSPMWQ 315

Query: 301 ----------------------------NENQDAVIGCSESPQASLRDKASKVMXXXXXX 360
                                       +EN DA+  C+ESPQ+SLR+KA+KV+      
Sbjct: 316 VRMKALCVLEAILRKKEDENFSIVHTYFSENLDAIQRCAESPQSSLREKANKVL-----S 375

Query: 361 XXXXXXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSV-----PLV-D 420
                                   DL D  D G            +  S      PL+ D
Sbjct: 376 LLNGGQSSGLMSSSDNTVKREAAVDLPDLIDTGXXXXXXXXXXXXDTGSTVATAGPLMDD 435

Query: 421 DLFGGGLNTVTSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDENKEPTS 480
           D FG   +   S+SE K DDDPF+DV FH  E +E+ DDLFSGM       T   K    
Sbjct: 436 DWFGDSSDIGLSSSEKKTDDDPFADVSFHPNEEKESADDLFSGM-------TVGEKSAAV 495

Query: 481 EQKNEPGVFDIFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFS 540
              + P +FD+FGS+++   +    K++ DLM   SI E+   +  KG S  +L + LF+
Sbjct: 496 GGNHVPDLFDMFGSTAKLEAEPKDAKNINDLMGSFSIDEN--NSNQKGSSSSTLPQDLFA 555

Query: 541 VSSQPNHQNQVPHDSLTGTYSSPMVGTNMNATFFPG--MPY-LPSGMMFNPAFSSQPMGY 600
           + S  +H  Q P + + G   S   G   N T  PG  MP+  P GMM NPAF+SQP+ Y
Sbjct: 556 MPSTTSH--QAPENPVGGILGSQNPGFIQN-TMLPGGVMPFNFPQGMMMNPAFASQPLNY 615

Query: 601 APTGNFFT-QQQLLSAMSNYQQFGNPNLQSSG--------GGYSSPLPDIFQPNLAAQSP 615
           A   +    QQQ L  MSN+QQFGN N Q SG        GG  S LPDIFQPN   Q+P
Sbjct: 616 AAMASLLAQQQQYLGNMSNFQQFGNLNAQGSGNVLSMGTSGGNQSALPDIFQPNFGNQAP 675

BLAST of Carg06471 vs. Swiss-Prot
Match: sp|Q3U3N6|AP4AT_MOUSE (AP-4 complex accessory subunit Tepsin OS=Mus musculus OX=10090 GN=Tepsin PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 4.6e-08
Identity = 41/123 (33.33%), Postives = 66/123 (53.66%), Query Frame = 0

Query: 6   TSDEDKVTPVYKLEEICEVLRSSHVSIVKE--FSEFILKRLEHKSPIVKQKALRLIKYAV 65
           TSD+    P Y  EEI ++   SH S+       E++L RL+  S  VK K L+++ Y  
Sbjct: 24  TSDDSIPCPGYLFEEIAKI---SHESLGSSQCLLEYLLNRLDSSSGHVKLKVLKILLYLC 83

Query: 66  GKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDNKPA 125
           G     F   ++R+S  +++   + G PDPL G++L + VR  A D  S +F++   +P 
Sbjct: 84  GHGSSSFLLILRRNSALIQEATAFSGPPDPLHGNSLYQKVRAAAQDLGSTLFSDAVPQP- 142

Query: 126 PSE 127
           PS+
Sbjct: 144 PSQ 142

BLAST of Carg06471 vs. Swiss-Prot
Match: sp|G3V8Y7|AP4AT_RAT (AP-4 complex accessory subunit Tepsin OS=Rattus norvegicus OX=10116 GN=Tepsin PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 6.1e-08
Identity = 41/123 (33.33%), Postives = 66/123 (53.66%), Query Frame = 0

Query: 6   TSDEDKVTPVYKLEEICEVLRSSHVSIVKE--FSEFILKRLEHKSPIVKQKALRLIKYAV 65
           TSD+D   P Y  EEI ++   SH S+       E++L RL+  S  VK K L+++ Y  
Sbjct: 24  TSDDDIPCPGYLFEEIAKI---SHESLGSSQCLLEYLLNRLDSSSGHVKLKVLKILLYLC 83

Query: 66  GKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDNKPA 125
                 F   ++R+S  +++   + G PDPL G++L + VR  A D  S +F++   +P 
Sbjct: 84  SHGSSSFMLILRRNSALIQEATAFAGPPDPLHGNSLYQKVRAAAQDLGSTLFSDALPQP- 142

Query: 126 PSE 127
           PS+
Sbjct: 144 PSQ 142

BLAST of Carg06471 vs. TrEMBL
Match: tr|A0A1S3BH26|A0A1S3BH26_CUCME (VHS domain-containing protein At3g16270 OS=Cucumis melo OX=3656 GN=LOC103489958 PE=4 SV=1)

HSP 1 Score: 968.0 bits (2501), Expect = 1.0e-278
Identity = 523/663 (78.88%), Postives = 549/663 (82.81%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSP+VKQKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPVVKQKALRLIK 75

Query: 61  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN 120
           YAVGKSGVEFRREMQR+SVAVRQLFHYKGQPDPLKGDALNKAVR+TAH+AIS+IFAEEDN
Sbjct: 76  YAVGKSGVEFRREMQRNSVAVRQLFHYKGQPDPLKGDALNKAVRDTAHEAISSIFAEEDN 135

Query: 121 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 180
           KPAPSENLNRRIQGFGNSNYEPP EDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT
Sbjct: 136 KPAPSENLNRRIQGFGNSNYEPPSEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 195

Query: 181 SSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNGNSSSGSSV 240
           SS RG NLQRSLTTE+EYDNRYEPVEYGRETLGT++STTSGTWNQDSRVSNG+ SSGSS 
Sbjct: 196 SSHRGINLQRSLTTEMEYDNRYEPVEYGRETLGTARSTTSGTWNQDSRVSNGSPSSGSSE 255

Query: 241 SKTREERLLETIATAGGVRLQPTRDAIQAFLVEAA------------------------- 300
           SKTRE+RLL+TIATAGGVRLQPTRD+IQAFLVEA                          
Sbjct: 256 SKTREDRLLDTIATAGGVRLQPTRDSIQAFLVEAVKLDALALSNALETKLKSPSWQVRFK 315

Query: 301 ------------------------NENQDAVIGCSESPQASLRDKASKVMXXXXXXXXXX 360
                                   +ENQ+AVIGCSESPQASLR+KASKVM          
Sbjct: 316 ALCILESIVRRNDDDHFSIVTSYFSENQEAVIGCSESPQASLREKASKVMPLLDGGKGVP 375

Query: 361 XXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFGGGLNTV 420
                               DL+DTSDAGDY GT+KS+EVENLSS PLVDDLFG GLNTV
Sbjct: 376 SMNVSEKSLPSNTSSTIQMPDLIDTSDAGDYSGTNKSVEVENLSSTPLVDDLFGDGLNTV 435

Query: 421 TSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDENKEPTSEQKNEPGVFD 480
           TSTSELKNDDDPFSDV FHT ETRENPDDLFSGMNF+NNQV++ENK+P  EQKNEPGVFD
Sbjct: 436 TSTSELKNDDDPFSDVSFHTIETRENPDDLFSGMNFDNNQVSNENKKPALEQKNEPGVFD 495

Query: 481 IFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQPNHQNQ 540
           IFGSSSEPAVQEHARKDV DLMSGLSIHED LK+KDKGDSKDSLSESLFS S QPNHQN 
Sbjct: 496 IFGSSSEPAVQEHARKDVNDLMSGLSIHEDTLKSKDKGDSKDSLSESLFSASGQPNHQNP 555

Query: 541 VPHDSLTGTYSSPMVGTNMNATFFPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFTQQQLL 600
           V  DSL G YSSPM GTNMNA FFPGM YLPSGMMFNPAFSSQPM YA +GNFFTQQQLL
Sbjct: 556 VSQDSLNGIYSSPMAGTNMNAAFFPGMTYLPSGMMFNPAFSSQPMAYAASGNFFTQQQLL 615

Query: 601 SAMSNYQQFGNPNLQSS------GGGYSSPLPDIFQPNLAAQSPSSVMNSSKKEDTRAFD 609
           SAMSNYQQFGNPNLQS+       GGYSSPLPDIFQPNLAAQS +SVMNSSKKEDTRAFD
Sbjct: 616 SAMSNYQQFGNPNLQSNSGGGVGSGGYSSPLPDIFQPNLAAQSSTSVMNSSKKEDTRAFD 675

BLAST of Carg06471 vs. TrEMBL
Match: tr|A0A0A0L8E2|A0A0A0L8E2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G462690 PE=4 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 1.9e-277
Identity = 521/664 (78.46%), Postives = 548/664 (82.53%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSP+VKQKALRLIK
Sbjct: 16  MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPVVKQKALRLIK 75

Query: 61  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN 120
           YAVGKSGVEFRREMQR+SVAVRQLFHYKGQPDPLKGDALNKAVR+TAH+AIS+IFAEEDN
Sbjct: 76  YAVGKSGVEFRREMQRNSVAVRQLFHYKGQPDPLKGDALNKAVRDTAHEAISSIFAEEDN 135

Query: 121 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 180
           KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT
Sbjct: 136 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 195

Query: 181 SSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNGNSSSGSSV 240
           SS RG NLQRSLTTE+EYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNG+ SSGSS 
Sbjct: 196 SSHRGINLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRVSNGSPSSGSSE 255

Query: 241 SKTREERLLETIATAGGVRLQPTRDAIQAFLVEAA------------------------- 300
           SKTRE+RLL+TIATAGGVRLQPTRD+IQAFLVEA                          
Sbjct: 256 SKTREDRLLDTIATAGGVRLQPTRDSIQAFLVEAVKLDALALSNALETKLKSPSWQVRFK 315

Query: 301 ------------------------NENQDAVIGCSESPQASLRDKASKVMXXXXXXXXXX 360
                                   +ENQ+AVIGCSESPQASLR+KA+KVM          
Sbjct: 316 ALCILESIVRRNDDDQFSIVTSYFSENQEAVIGCSESPQASLREKATKVMPLLDGGKGVP 375

Query: 361 XXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSVPLVDDLFGGGLNTV 420
                               DL+DTSDAGD+ GT+KS+EVENLSS PLVDDLFG GLNTV
Sbjct: 376 SINVYEKSLPSNTSSTIQMPDLIDTSDAGDFSGTNKSVEVENLSSTPLVDDLFGDGLNTV 435

Query: 421 TSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDENKEPTSEQKNEPGVFD 480
           TSTSELKNDDDPFSDV FHT ETRENPDDLFSGMNF+NNQV++ENK+   E KNEPGVFD
Sbjct: 436 TSTSELKNDDDPFSDVSFHTIETRENPDDLFSGMNFDNNQVSNENKKAALEPKNEPGVFD 495

Query: 481 IFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSESLFSVSSQPNHQNQ 540
           IFGSSSEPAVQEHARKDV DLMSGLSIHED LK KDKGDSKDSLSESLFS S+QPNHQNQ
Sbjct: 496 IFGSSSEPAVQEHARKDVNDLMSGLSIHEDILKGKDKGDSKDSLSESLFSASAQPNHQNQ 555

Query: 541 VPHDSLTGTYSSPMVGTNMNATFFPGMPYLPSGMMFNPAFSSQPMGYAPTGNFFTQQQLL 600
           V  DSL G YSSPM G+NMNA FFPGM YLPSGM+FNPAFSSQPM YA TGNFFTQQQLL
Sbjct: 556 VSQDSLNGIYSSPMAGSNMNAAFFPGMTYLPSGMVFNPAFSSQPMAYAATGNFFTQQQLL 615

Query: 601 SAMSNYQQFGNPNLQSS-------GGGYSSPLPDIFQPNLAAQSPSSVMNSSKKEDTRAF 609
           SAMSNYQQFGNPNLQS+        GGYSSP PDIFQPNLAAQS +SVMNSSKKEDTRAF
Sbjct: 616 SAMSNYQQFGNPNLQSNSGGGGVGSGGYSSPFPDIFQPNLAAQSSTSVMNSSKKEDTRAF 675

BLAST of Carg06471 vs. TrEMBL
Match: tr|A0A1R3H8F7|A0A1R3H8F7_9ROSI (Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_30474 PE=4 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 3.4e-181
Identity = 386/688 (56.10%), Postives = 462/688 (67.15%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MI+ ATSDEDKV PVYKLEEICE+LRSSHVSIVKE SEFILKRL+HKSPIVKQK LRLIK
Sbjct: 16  MIEGATSDEDKVAPVYKLEEICELLRSSHVSIVKEVSEFILKRLDHKSPIVKQKVLRLIK 75

Query: 61  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEE-- 120
           YAVGKSGVEFRREMQR+SVAVRQLFHYKGQPDPLKGDALNKAVRETAH+AISAIFAEE  
Sbjct: 76  YAVGKSGVEFRREMQRNSVAVRQLFHYKGQPDPLKGDALNKAVRETAHEAISAIFAEENS 135

Query: 121 DNKPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKN 180
           ++KP+ S++LN+RIQGFGN+N+E P +DKKSFLSEVVG+GSASIKQG+S+F Q HS RKN
Sbjct: 136 NSKPSTSDDLNKRIQGFGNTNFEMPSDDKKSFLSEVVGIGSASIKQGISSFTQSHSLRKN 195

Query: 181 GTSSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTTSGTWNQDSRV-----SNGN 240
              S + P L+RSLTTEI++ +RYEPVE   ET G S++TTSG W QDSRV     +NG 
Sbjct: 196 DNGSYKSPTLRRSLTTEIDHSDRYEPVELPNETQGVSRNTTSGPWGQDSRVLKTETTNGQ 255

Query: 241 SSSGSSVSKTREERLLETIATAGGVRLQPTRDAIQAFLVEAA------------------ 300
           SSS  S +KTREERLLETI T+GGVRLQPTRDAIQAFLVEAA                  
Sbjct: 256 SSSNYSETKTREERLLETIVTSGGVRLQPTRDAIQAFLVEAAKLDALALSYALESKLQSQ 315

Query: 301 -------------------------------NENQDAVIGCSESPQASLRDKASKVMXXX 360
                                           ENQD V+ CSESPQASLR+KA+KV+   
Sbjct: 316 MWQVRMKAVCVLESILRKKEDEHFSVVASYFTENQDVVLRCSESPQASLREKANKVL-IL 375

Query: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVE------NLSSVP 420
                                      +L+DT D  DY G D S + +      NL++ P
Sbjct: 376 LGGEQTSGLARNSEKSLKAETTPVEMPNLIDTGDPDDYNGLDDSTKSQYDQNTVNLTATP 435

Query: 421 LVDDLFGGGLNTVTSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDENKE 480
           L+DDL G GL++  STSE +NDDDPF+DV FH  E REN DDLFSGM  ++  V + N  
Sbjct: 436 LIDDLLGDGLDSSLSTSEQRNDDDPFADVSFHAGEGRENVDDLFSGMTLDDKSVVNGNHA 495

Query: 481 PTSEQKNEPGVFDIFGSSSE-PAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDSLSE 540
             ++    P + DIFG++SE P  QE+ + DV DLM+GLS++E++     KG S ++  E
Sbjct: 496 AANK---NPELIDIFGNNSEAPFEQENKKNDVNDLMAGLSMNENSSNLMQKGVSSEANPE 555

Query: 541 SLFSVSSQPNHQNQVPHDSLTGTYSSPMVGTNMNATFFPG-MPY-LPSGMMFNPAFSSQP 600
           +LFS     N   Q  +D+ +G   S   G N N  F  G MPY +P G+MFNPAF+SQP
Sbjct: 556 NLFS-----NINIQASNDASSGVLGSQASGMNANPMFPLGTMPYSIPPGIMFNPAFASQP 615

Query: 601 MGYAPTGNFFTQQQLLSAMSNYQQFGNPNLQSSG---------GGYSSPLPDIFQPNLAA 615
           M YA  GNFF QQQLL+ M+N Q  GN N Q++G         GGYSSP+PDIFQ N   
Sbjct: 616 MNYAAMGNFFAQQQLLATMANLQHLGNFNSQNAGMSHVSSGSNGGYSSPIPDIFQSNFPT 675

BLAST of Carg06471 vs. TrEMBL
Match: tr|D7U0F4|D7U0F4_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_09s0002g03990 PE=4 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 4.9e-180
Identity = 386/691 (55.86%), Postives = 456/691 (65.99%), Query Frame = 0

Query: 1   MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
           MID ATSDEDKVTPVYKLEEICE+LRSSHVSIVKE SEFILKRL+HKSP+V+QKALRLIK
Sbjct: 16  MIDGATSDEDKVTPVYKLEEICELLRSSHVSIVKEVSEFILKRLDHKSPVVRQKALRLIK 75

Query: 61  YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN 120
           YAVGKSGVEFRREMQRHSVAVRQL HYKGQ D LKGDALNKAVR+TA + ISAIFA E+N
Sbjct: 76  YAVGKSGVEFRREMQRHSVAVRQLLHYKGQLDALKGDALNKAVRDTAQETISAIFAAEEN 135

Query: 121 KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 180
           KPAPSE+LNRRIQGFGN+NYE P EDKKSFLSEVVGLGS SIKQG+S+  QGHS RKN  
Sbjct: 136 KPAPSEDLNRRIQGFGNTNYEMPSEDKKSFLSEVVGLGSTSIKQGISSITQGHSLRKNDN 195

Query: 181 SSPRGPNLQRSLTTEIEYDNRYEPVEYGRET---LGTSKSTTSGTWNQDSRV-----SNG 240
            S + PNL+RSLTTEIEY +RYE VE+  ET    G+SK+ + G WNQD R+     +NG
Sbjct: 196 GSYKSPNLRRSLTTEIEYQDRYEGVEHQNETQSSFGSSKNVSGGPWNQDVRITKSETTNG 255

Query: 241 NSSSGSSVSKTREERLLETIATAGGVRLQPTRDAIQAFLVEAAN---------------- 300
           +SSS  + SKTREERLLETI T+GGVRLQPTRDAIQ FL+EAA                 
Sbjct: 256 DSSSSFAESKTREERLLETIVTSGGVRLQPTRDAIQIFLIEAAKLDALALSHALESKLHS 315

Query: 301 ---------------------------------ENQDAVIGCSESPQASLRDKASKVMXX 360
                                            EN+D V+ C ESPQASLR+KA+KV+  
Sbjct: 316 PLWQVRMKAVCVLDSILRKKDEEQFSTVASYFCENKDVVLKCCESPQASLREKANKVLSL 375

Query: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSV------ 420
                                       DL+DT    DY G + S++ ++  SV      
Sbjct: 376 LGGEQTGSTMSPPEKPLKTETVPAVQMPDLIDTGGPDDYYGAEDSIKTQSDQSVPNLTTS 435

Query: 421 --PLVDDLFGGGLNTVTSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDE 480
             PL+DDLFG GL    +T E KNDDDPF+DV F + E RE  DDLFSGM  ++      
Sbjct: 436 TSPLIDDLFGDGLGVSENTGEQKNDDDPFADVSFLSGEAREQVDDLFSGMTVDDK--VGA 495

Query: 481 NKEPTSEQKNEPGVFDIFGSSSE-PAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDS 540
           N+ P +   NEP +FDIFGS++E P  QE+ +KDV DLM+GLSI+ +      KG    +
Sbjct: 496 NEIPKAATSNEPELFDIFGSNAELPQEQENHKKDVNDLMAGLSINGNVSNTTQKGMLPGA 555

Query: 541 LSESLFSVSSQPNHQNQVPHDSLTGTYSSPMVGTNMNATFFPG-MPY-LPSGMMFNPAFS 600
           LSE++FS  S      QV +D+L G + S   G N NA F  G MPY +P G+MFNPAF 
Sbjct: 556 LSETVFS-DSNSYSPKQVSNDALNG-FGSETAGMNANAMFPLGSMPYNIPPGIMFNPAFP 615

Query: 601 SQPMGYAPTGNFFTQQQLLSAMSNYQQFGNPNLQSSG---------GGYSSPLPDIFQPN 615
           SQPM Y   GNFF QQQ L+AMSN+QQ GN   QS+G         GGYSSPLPDIF P+
Sbjct: 616 SQPMNYGAMGNFFAQQQFLAAMSNFQQLGNLTSQSAGMGHAGGTMEGGYSSPLPDIFHPH 675

BLAST of Carg06471 vs. TrEMBL
Match: tr|A5BML7|A5BML7_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_011432 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 8.3e-180
Identity = 386/691 (55.86%), Postives = 455/691 (65.85%), Query Frame = 0

Query: 1    MIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLIK 60
            MID ATSDEDKVTPVYKLEEICE+LRSSHVSIVKE SEFILKRL+HKSP+V+QKALRLIK
Sbjct: 1001 MIDGATSDEDKVTPVYKLEEICELLRSSHVSIVKEVSEFILKRLDHKSPVVRQKALRLIK 1060

Query: 61   YAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRETAHDAISAIFAEEDN 120
            YAVGKSGVEFRREMQRHSVAVRQL HYKGQ D LKGDALNKAVR+TA + ISAIFA E+N
Sbjct: 1061 YAVGKSGVEFRREMQRHSVAVRQLLHYKGQLDALKGDALNKAVRDTAQETISAIFAAEEN 1120

Query: 121  KPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQGLSNFAQGHSSRKNGT 180
            KPAPSE+LNRRIQGFGN+NYE P EDKKSFLSEVVGLGS SIKQG+S+  QGHS RKN  
Sbjct: 1121 KPAPSEDLNRRIQGFGNTNYEMPSEDKKSFLSEVVGLGSTSIKQGISSITQGHSLRKNDN 1180

Query: 181  SSPRGPNLQRSLTTEIEYDNRYEPVEYGRET---LGTSKSTTSGTWNQDSRV-----SNG 240
             S + PNL+RSLTTEIEY +RYE VE+  ET    G+SK+ + G WNQD R+     +NG
Sbjct: 1181 GSYKSPNLRRSLTTEIEYQDRYEGVEHQNETQSSFGSSKNVSGGPWNQDVRITKSETTNG 1240

Query: 241  NSSSGSSVSKTREERLLETIATAGGVRLQPTRDAIQAFLVEAAN---------------- 300
            +SSS  + SKTREERLLETI T+GGVRLQPTRDAIQ FL+EAA                 
Sbjct: 1241 DSSSSFAESKTREERLLETIVTSGGVRLQPTRDAIQIFLIEAAKLDALALSHALESKLHS 1300

Query: 301  ---------------------------------ENQDAVIGCSESPQASLRDKASKVMXX 360
                                             EN+D V+ C ESPQASLR+KA+KV+  
Sbjct: 1301 PLWQVRMKAVCVLDSILRKKDEEQFSTVASYFCENKDVVLKCCESPQASLREKANKVLSL 1360

Query: 361  XXXXXXXXXXXXXXXXXXXXXXXXXXXXDLLDTSDAGDYGGTDKSLEVENLSSV------ 420
                                        DL+DT    DY G + S++ ++  SV      
Sbjct: 1361 LGGEQTGSTMSPPEKPLKTETVPAVQMPDLIDTGGPDDYYGAEDSIKTQSDQSVPNLTTS 1420

Query: 421  --PLVDDLFGGGLNTVTSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQVTDE 480
              PL+DDLFG GL    +T E KNDDDPF+DV F + E RE  DDLFSGM  ++      
Sbjct: 1421 TSPLIDDLFGDGLGVSENTGEQKNDDDPFADVSFLSGEAREQVDDLFSGMTVDDK--VGA 1480

Query: 481  NKEPTSEQKNEPGVFDIFGSSSE-PAVQEHARKDVIDLMSGLSIHEDALKNKDKGDSKDS 540
            N+ P +   NEP +FDIFGS++E P  QE+ +KDV DLM+GLSI+ +      KG    +
Sbjct: 1481 NEIPKAATSNEPELFDIFGSNAELPQEQENHKKDVNDLMAGLSINGNVSNTTQKGMLPGA 1540

Query: 541  LSESLFSVSSQPNHQNQVPHDSLTGTYSSPMVGTNMNATFFPG-MPY-LPSGMMFNPAFS 600
            LSE +FS  S      QV +D+L G + S   G N NA F  G MPY +P G+MFNPAF 
Sbjct: 1541 LSEXVFS-DSNSYSPKQVSNDALNG-FGSETAGMNANAMFPLGSMPYNIPPGIMFNPAFP 1600

Query: 601  SQPMGYAPTGNFFTQQQLLSAMSNYQQFGNPNLQSSG---------GGYSSPLPDIFQPN 615
            SQPM Y   GNFF QQQ L+AMSN+QQ GN   QS+G         GGYSSPLPDIF P+
Sbjct: 1601 SQPMNYGAMGNFFAQQQFLAAMSNFQQLGNLTSQSAGMGHAGGTMEGGYSSPLPDIFHPH 1660

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022979433.11.9e-31091.93VHS domain-containing protein At3g16270-like [Cucurbita maxima][more]
XP_023526954.15.7e-31091.93VHS domain-containing protein At3g16270-like [Cucurbita pepo subsp. pepo][more]
XP_022955654.16.4e-30991.63VHS domain-containing protein At3g16270-like [Cucurbita moschata][more]
XP_008447533.11.5e-27878.88PREDICTED: VHS domain-containing protein At3g16270 [Cucumis melo] >XP_008447534.... [more]
XP_004151053.12.9e-27778.46PREDICTED: VHS domain-containing protein At3g16270 [Cucumis sativus] >KGN58048.1... [more]
Match NameE-valueIdentityDescription
AT3G16270.11.9e-15350.66ENTH/VHS family protein[more]
Match NameE-valueIdentityDescription
sp|Q9C5H4|MTV1_ARATH3.4e-15250.66Protein MODIFIED TRANSPORT TO THE VACUOLE 1 OS=Arabidopsis thaliana OX=3702 GN=M... [more]
sp|Q3U3N6|AP4AT_MOUSE4.6e-0833.33AP-4 complex accessory subunit Tepsin OS=Mus musculus OX=10090 GN=Tepsin PE=2 SV... [more]
sp|G3V8Y7|AP4AT_RAT6.1e-0833.33AP-4 complex accessory subunit Tepsin OS=Rattus norvegicus OX=10116 GN=Tepsin PE... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3BH26|A0A1S3BH26_CUCME1.0e-27878.88VHS domain-containing protein At3g16270 OS=Cucumis melo OX=3656 GN=LOC103489958 ... [more]
tr|A0A0A0L8E2|A0A0A0L8E2_CUCSA1.9e-27778.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G462690 PE=4 SV=1[more]
tr|A0A1R3H8F7|A0A1R3H8F7_9ROSI3.4e-18156.10Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_30474 PE=4 SV=1[more]
tr|D7U0F4|D7U0F4_VITVI4.9e-18055.86Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_09s0002g03990 PE=4 SV=... [more]
tr|A5BML7|A5BML7_VITVI8.3e-18055.86Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_011432 PE=4 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006886intracellular protein transport
Vocabulary: INTERPRO
TermDefinition
IPR039273TEPSIN
IPR008942ENTH_VHS
IPR013809ENTH
IPR002014VHS_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg06471-RACarg06471-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002014VHS domainSMARTSM00288VHS_2coord: 1..132
e-value: 5.0E-23
score: 92.5
IPR002014VHS domainPROSITEPS50179VHScoord: 5..74
score: 10.058
IPR013809ENTH domainPFAMPF01417ENTHcoord: 2..118
e-value: 1.3E-5
score: 25.1
IPR013809ENTH domainPROSITEPS50942ENTHcoord: 1..124
score: 8.592
IPR008942ENTH/VHSGENE3DG3DSA:1.25.40.90coord: 1..125
e-value: 4.3E-47
score: 161.8
IPR008942ENTH/VHSSUPERFAMILYSSF48464ENTH/VHS domaincoord: 2..120
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 463..496
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 475..496
NoneNo IPR availablePANTHERPTHR21514:SF3SUBFAMILY NOT NAMEDcoord: 277..613
coord: 1..275
IPR039273AP-4 complex accessory subunit TepsinPANTHERPTHR21514UNCHARACTERIZEDcoord: 277..613
coord: 1..275

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg06471Carg04271Silver-seed gourdcarcarB139