Carg05276 (gene) Silver-seed gourd

Overview
NameCarg05276
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionBrassinosteroid receptor BRI1
LocationCucurbita_argyrosperma_scaffold_010 : 878767 .. 880629 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGAACGGAACGATTCCTCCGGAGCTGTTTCTACAATCGGGTAACATTGCTGTAAACTTTATCACTGGGAAGTCTTATGCTTACATTAAGAATGATGGTAGCAAGCAGTGCCATGGAGCTGGAAATTTGGTGGAGTTTGCTGGGATAAGACAAGAACAAGTGAACAGGATATCAAGTAAGAGTCCCTGCAATTTCACCAGGGTTTATAAAGGAATGACTCAACCGACGTTTAACCATAACGGGTCGATGATCTTCCTCGATCTTTCGCATAATATGTTGTCTGGTAGTATTCCAAAGGAGATTGGTTCTACAAAATATCTGTACATATTGGATTTGGGGCACAACAGGGTGTCGGGAGGCATTCCGCAGGAGCTGGGCGACTTGACGAAGCTTAACATTCTTGATCTGTCAAGCAATGAGCTTGAAGGATCAATACCATTGTCCTTGACTGGGCTATCCTCCCTCATGGAGATTGATCTGTCAAACAATCATCTCAATGGTTCGATACCCGAATCAGCTCAGTTCGAAACGTTCCCGGCATCTGGATTTGCAAATAATTCTGGCCTCTGTGGGTATCCTCTGCCTCAATGCAGGGTTGATTCAGCAGCAAATGCAAACTCTCAGCATCAGAGATCTCATAGGAAACAGGCATCGCTTGCAGGGAGCGTCGCGATGGGGCTACTATTCTCCCTCTTCTGTATATTCGGTCTGATCATAGTCGTTATCGAGATGAAGAAGAGAAGGAAAAACAAGGATGCTGCTCTTGATTCTTATATTGACACTCATTCCCAGTCGGGCAACACAACCACGGTGAACTGGAAGAACACCTGTGTTCGTGAAGCATTGAGCATCAATCTTGCAACATTCGAGAAGCCACTTCGGAAGCTTACGTTTGCAGATCTTCTCGACGCCACCAATGGCTTCCACGACAATACGCTCATTGGTTCGGGGGGTTTCGGGGACGTATATAAGGCTCAATTGAAGGATGGAAGCATTGTAGCCATCAAGAAGCTGATTCATGTCAGTGGACAGGGTGATAGGGAGTTCACTGCAGAAATGGAAACCATTGGCAAAATCAAACACCGAAACCTCGTACCTCTTCTCGGCTACTGCAAAGTCGGAGATGAACGCCTTCTTGTGTACGAATACATGAAATATGGAAGCTTGGAAGACGTTTTACACGACCATAACAAGGCTGGAATCAAACTCAATTGGGCTGCTAGAAGAAAGATTGCCATTGGAGCTGCTAGGGGCCTGGCTTTCCTTCACCACAATTGCATCCCTCACATCATCCACAGGGACATGAAATCCAGCAATGTCTTATTGGATGAGAACTTGGAAGCCAGAGTCTCTGATTTTGGTATGGCTCGACTCATGAGTGCTATGGACACCCATTTGAGTGTCAGCACATTAGCCGGTACCCCCGGTTACGTCCCTCCCGAATATTACCAAAGCTTCCGCTGTTCGACCAAAGGCGACGTGTACAGCTACGGCGTCGTTATGCTCGAGCTTTTGACAGGAAAACGACCCACAGATTCTGCCGATTTTGGTGACAACAACCTCGTCGGATGGGTTAAACAACACGCCAAGTTGGACCTAACCAATGTCTTCGATCCCGAGCTCTTGAAGGAAGATCCCAACCTTAAGATAGAGCTTTTAGAACACTTGAAAGTAGCTGTTGCTTGCTTAGATGATAGGTCCTGGCGGCGTCCAACCATGATCCAAGTGATGACGATGTTCAAGGAAATCCAAGCTGGGTCGGGTATGGATTCGCAATCTACCATCGGATCCGACAACGGTGGATTCAGCATCGACATGGTAGACATGAGCTTAAAGGAAGTACCAGAAGGCAAGTACTAG

mRNA sequence

ATGCTGAACGGAACGATTCCTCCGGAGCTGTTTCTACAATCGGGTAACATTGCTGTAAACTTTATCACTGGGAAGTCTTATGCTTACATTAAGAATGATGGTAGCAAGCAGTGCCATGGAGCTGGAAATTTGGTGGAGTTTGCTGGGATAAGACAAGAACAAGTGAACAGGATATCAAGTAAGAGTCCCTGCAATTTCACCAGGGTTTATAAAGGAATGACTCAACCGACGTTTAACCATAACGGGTCGATGATCTTCCTCGATCTTTCGCATAATATGTTGTCTGGTAGTATTCCAAAGGAGATTGGTTCTACAAAATATCTGTACATATTGGATTTGGGGCACAACAGGGTGTCGGGAGGCATTCCGCAGGAGCTGGGCGACTTGACGAAGCTTAACATTCTTGATCTGTCAAGCAATGAGCTTGAAGGATCAATACCATTGTCCTTGACTGGGCTATCCTCCCTCATGGAGATTGATCTGTCAAACAATCATCTCAATGGTTCGATACCCGAATCAGCTCAGTTCGAAACGTTCCCGGCATCTGGATTTGCAAATAATTCTGGCCTCTGTGGGTATCCTCTGCCTCAATGCAGGGTTGATTCAGCAGCAAATGCAAACTCTCAGCATCAGAGATCTCATAGGAAACAGGCATCGCTTGCAGGGAGCGTCGCGATGGGGCTACTATTCTCCCTCTTCTGTATATTCGGTCTGATCATAGTCGTTATCGAGATGAAGAAGAGAAGGAAAAACAAGGATGCTGCTCTTGATTCTTATATTGACACTCATTCCCAGTCGGGCAACACAACCACGGTGAACTGGAAGAACACCTGTGTTCGAAAACGACCCACAGATTCTGCCGATTTTGGTGACAACAACCTCGTCGGATGGGTTAAACAACACGCCAAGTTGGACCTAACCAATGTCTTCGATCCCGAGCTCTTGAAGGAAGATCCCAACCTTAAGATAGAGCTTTTAGAACACTTGAAAGTAGCTGTTGCTTGCTTAGATGATAGGTCCTGGCGGCGTCCAACCATGATCCAAGTGATGACGATGTTCAAGGAAATCCAAGCTGGGTCGGGTATGGATTCGCAATCTACCATCGGATCCGACAACGGTGGATTCAGCATCGACATGGTAGACATGAGCTTAAAGGAAGTACCAGAAGGCAAGTACTAG

Coding sequence (CDS)

ATGCTGAACGGAACGATTCCTCCGGAGCTGTTTCTACAATCGGGTAACATTGCTGTAAACTTTATCACTGGGAAGTCTTATGCTTACATTAAGAATGATGGTAGCAAGCAGTGCCATGGAGCTGGAAATTTGGTGGAGTTTGCTGGGATAAGACAAGAACAAGTGAACAGGATATCAAGTAAGAGTCCCTGCAATTTCACCAGGGTTTATAAAGGAATGACTCAACCGACGTTTAACCATAACGGGTCGATGATCTTCCTCGATCTTTCGCATAATATGTTGTCTGGTAGTATTCCAAAGGAGATTGGTTCTACAAAATATCTGTACATATTGGATTTGGGGCACAACAGGGTGTCGGGAGGCATTCCGCAGGAGCTGGGCGACTTGACGAAGCTTAACATTCTTGATCTGTCAAGCAATGAGCTTGAAGGATCAATACCATTGTCCTTGACTGGGCTATCCTCCCTCATGGAGATTGATCTGTCAAACAATCATCTCAATGGTTCGATACCCGAATCAGCTCAGTTCGAAACGTTCCCGGCATCTGGATTTGCAAATAATTCTGGCCTCTGTGGGTATCCTCTGCCTCAATGCAGGGTTGATTCAGCAGCAAATGCAAACTCTCAGCATCAGAGATCTCATAGGAAACAGGCATCGCTTGCAGGGAGCGTCGCGATGGGGCTACTATTCTCCCTCTTCTGTATATTCGGTCTGATCATAGTCGTTATCGAGATGAAGAAGAGAAGGAAAAACAAGGATGCTGCTCTTGATTCTTATATTGACACTCATTCCCAGTCGGGCAACACAACCACGGTGAACTGGAAGAACACCTGTGTTCGAAAACGACCCACAGATTCTGCCGATTTTGGTGACAACAACCTCGTCGGATGGGTTAAACAACACGCCAAGTTGGACCTAACCAATGTCTTCGATCCCGAGCTCTTGAAGGAAGATCCCAACCTTAAGATAGAGCTTTTAGAACACTTGAAAGTAGCTGTTGCTTGCTTAGATGATAGGTCCTGGCGGCGTCCAACCATGATCCAAGTGATGACGATGTTCAAGGAAATCCAAGCTGGGTCGGGTATGGATTCGCAATCTACCATCGGATCCGACAACGGTGGATTCAGCATCGACATGGTAGACATGAGCTTAAAGGAAGTACCAGAAGGCAAGTACTAG

Protein sequence

MLNGTIPPELFLQSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVYKGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYLYILDLGHNRVSGGIPQELGDLTKLNILDLSSNELEGSIPLSLTGLSSLMEIDLSNNHLNGSIPESAQFETFPASGFANNSGLCGYPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRKNKDAALDSYIDTHSQSGNTTTVNWKNTCVRKRPTDSADFGDNNLVGWVKQHAKLDLTNVFDPELLKEDPNLKIELLEHLKVAVACLDDRSWRRPTMIQVMTMFKEIQAGSGMDSQSTIGSDNGGFSIDMVDMSLKEVPEGKY
Homology
[Close]
BLAST of Carg05276 vs. NCBI nr
Match: XP_023518235.1 (protein BRASSINOSTEROID INSENSITIVE 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521.5 bits (1342), Expect = 2.4e-144
Identity = 381/610 (62.46%), Postives = 381/610 (62.46%), Query Frame = 0 

Query: 11   FLQSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVY 70
            F QSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVY
Sbjct: 581  FRQSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVY 640

Query: 71   KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXX 130
            KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 641  KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXX 700

Query: 131  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL 190
            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL
Sbjct: 701  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL 760

Query: 191  CGYPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRK 250
            CGYPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRK
Sbjct: 761  CGYPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRK 820

Query: 251  NKDAALDSYIDTHSQSGNTTTVNWKNTCVR------------------------------ 310
            NKDAALDSYIDTHSQSGNTTTVNWKNTCVR                              
Sbjct: 821  NKDAALDSYIDTHSQSGNTTTVNWKNTCVREALSINLATFEKPLRKLTFADLLDATNGFH 880

Query: 311  ------------------------------------------------------------ 370
                                                                        
Sbjct: 881  DNTLIGSGGFGDVYKAQLKDGSIVAIKKLIHVSGQGDREFTAEMETIGKIKHRNLVPLLG 940

Query: 371  ------------------------------------------------------------ 393
                                                                        
Sbjct: 941  YCKVGDERLLVYDYMKYGSLEDVLHDHNKAGIKLNWAARRKIAIGAARGLAFLHHNCIPH 1000

[Close]
BLAST of Carg05276 vs. NCBI nr
Match: XP_022963139.1 (protein BRASSINOSTEROID INSENSITIVE 1 [Cucurbita moschata])

HSP 1 Score: 518.8 bits (1335), Expect = 1.6e-143
Identity = 379/608 (62.34%), Postives = 380/608 (62.50%), Query Frame = 0 

Query: 13   QSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVYKG 72
            QSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVYKG
Sbjct: 583  QSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVYKG 642

Query: 73   MTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXXXX 132
            MTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 643  MTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXXXX 702

Query: 133  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGLCG 192
            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGLCG
Sbjct: 703  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGLCG 762

Query: 193  YPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRKNK 252
            YPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRKNK
Sbjct: 763  YPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRKNK 822

Query: 253  DAALDSYIDTHSQSGNTTTVNWKNTCVR-------------------------------- 312
            DAALDSYIDTHSQSGNTTTVNWKNTCVR                                
Sbjct: 823  DAALDSYIDTHSQSGNTTTVNWKNTCVREALSINLATFEKPLRKLTFADLLDATNGFHDN 882

Query: 313  ------------------------------------------------------------ 372
                                                                        
Sbjct: 883  TLIGSGGFGDVYKAQLKDGSIVAIKKLIHVSGQGDREFTAEMETIGKIKHRNLVPLLGYC 942

Query: 373  ------------------------------------------------------------ 393
                                                                        
Sbjct: 943  KVGDERLLVYEYMKYGSLEDVLHDHNKAGIKLNWAARRKIAIGAARGLAFLHHNCIPHII 1002

[Close]
BLAST of Carg05276 vs. NCBI nr
Match: XP_023003428.1 (protein BRASSINOSTEROID INSENSITIVE 1 [Cucurbita maxima])

HSP 1 Score: 515.0 bits (1325), Expect = 2.2e-142
Identity = 377/610 (61.80%), Postives = 379/610 (62.13%), Query Frame = 0 

Query: 11   FLQSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVY 70
            F QSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVY
Sbjct: 581  FRQSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVY 640

Query: 71   KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXX 130
            KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 641  KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXX 700

Query: 131  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL 190
            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL
Sbjct: 701  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL 760

Query: 191  CGYPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRK 250
            CGYPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRK
Sbjct: 761  CGYPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRK 820

Query: 251  NKDAALDSYIDTHSQSGNTTTVNWKNTCVR------------------------------ 310
            NKD+ALDSYIDTHS SG TTTVNWKNTCVR                              
Sbjct: 821  NKDSALDSYIDTHSHSGTTTTVNWKNTCVREALSINLATFEKPLRKLTFADLLDATNGFH 880

Query: 311  ------------------------------------------------------------ 370
                                                                        
Sbjct: 881  DNTLIGSGGFGDVYKAQLKDGSIVAIKKLIHVSGQGDREFTAEMETIGKIKHRNLVPLLG 940

Query: 371  ------------------------------------------------------------ 393
                                                                        
Sbjct: 941  YCKVGDERLLVYDYMKYGSLEDVLHDHNKAGIKLNWAARRKIAIGAARGLAFLHHNCIPH 1000

[Close]
BLAST of Carg05276 vs. NCBI nr
Match: XP_008440121.1 (PREDICTED: protein BRASSINOSTEROID INSENSITIVE 1 [Cucumis melo])

HSP 1 Score: 433.3 bits (1113), Expect = 8.6e-118
Identity = 338/607 (55.68%), Postives = 349/607 (57.50%), Query Frame = 0 

Query: 11   FLQSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVY 70
            F QSGNIAVNFITGKSYAYIKNDGSKQCHGAGNL+EFAGIRQEQV+RISSKSPCNFTRVY
Sbjct: 594  FRQSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLLEFAGIRQEQVSRISSKSPCNFTRVY 653

Query: 71   KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXX 130
            KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGST  XXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 654  KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTXXXXXXXXXXXXXXXXXXXXXXXXX 713

Query: 131  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL 190
            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL
Sbjct: 714  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL 773

Query: 191  CGYPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRK 250
            CGYPLP C VDSA NANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLI           
Sbjct: 774  CGYPLPPCVVDSAGNANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIXXXXXXXXXXX 833

Query: 251  NKDAALDSYIDTHSQSGNTTTVNWKNTCVR------------------------------ 310
               + LDSY+++HSQSG TT VNWK T  R                              
Sbjct: 834  XXXSTLDSYVESHSQSGTTTAVNWKLTGAREALSINLATFEKPLRKLTFADLLEATNGFH 893

Query: 311  ------------------------------------------------------------ 370
                                                                        
Sbjct: 894  NDSMIGSGGFGDVYKAQLKDGSTVAIKKLIHVSGQGDREFTAEMETIGKIKHRNLVPLLG 953

Query: 371  ------------------------------------------------------------ 390
                                                                        
Sbjct: 954  YCKVGEERLLVYEYMKYGSLEDVLHDQKKGGIKLNWSARRKIAIGAARGLAFLHHNCIPH 1013

[Close]
BLAST of Carg05276 vs. NCBI nr
Match: XP_023544828.1 (systemin receptor SR160-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 431.0 bits (1107), Expect = 4.3e-117
Identity = 338/606 (55.78%), Postives = 352/606 (58.09%), Query Frame = 0 

Query: 13   QSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVYKG 72
            QSGNIAVNFITGKSYAYIKNDGSKQCHGAGNL+EFAGIRQEQVNRISSKSPCNFTRVYKG
Sbjct: 589  QSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLLEFAGIRQEQVNRISSKSPCNFTRVYKG 648

Query: 73   MTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXXXX 132
            MTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGST  XXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 649  MTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTXXXXXXXXXXXXXXXXXXXXXXXXXXX 708

Query: 133  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGLCG 192
            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGLCG
Sbjct: 709  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGLCG 768

Query: 193  YPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRKNK 252
            YPLP C VDS A+ANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLI            K
Sbjct: 769  YPLPSCGVDSGASANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIXXXXXXXXXXXXK 828

Query: 253  DAALDSYIDTHSQSGNTTTVNWKNTCVR-------------------------------- 312
            D+ LD+Y+++HS  G TTTVNWK T  R                                
Sbjct: 829  DSTLDTYVESHSPPGTTTTVNWKLTGAREASSISLATFEKPLRKLTFADLLKATNGFHND 888

Query: 313  ------------------------------------------------------------ 372
                                                                        
Sbjct: 889  SLIGSGGFGDVYKARLKDGSTVAIKKLIHVSGQGDREFTAEMETIGKIKHRNLVPLLGYC 948

Query: 373  ------------------------------------------------------------ 390
                                                                        
Sbjct: 949  KVGEERLLVYEYMKYGSLEDVLHDKKKGGIKLNWAARRKIAIGAARGLAFLHHNCLPHII 1008

[Close]
BLAST of Carg05276 vs. TAIR10
Match: AT4G39400.1 (Leucine-rich receptor-like protein kinase family protein)

HSP 1 Score: 301.2 bits (770), Expect = 9.2e-82
Identity = 285/616 (46.27%), Postives = 320/616 (51.95%), Query Frame = 0 

Query: 11   FLQSGNIAVNFITGKSYAYIKNDG-SKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFT-R 70
            F QSG IA NFI GK Y YIKNDG  K+CHGAGNL+EF GIR EQ+NR+S+++PCN T R
Sbjct: 581  FKQSGKIAANFIAGKRYVYIKNDGMKKECHGAGNLLEFQGIRSEQLNRLSTRNPCNITSR 640

Query: 71   VYKGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXX 130
            VY G T PTF++NGSM+FLD+S+NMLSG IPKEIGS   XXXXXXXXXXXXXXXXXXXXX
Sbjct: 641  VYGGHTSPTFDNNGSMMFLDMSYNMLSGYIPKEIGSMXXXXXXXXXXXXXXXXXXXXXXX 700

Query: 131  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNS 190
            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   ETFP + F NN 
Sbjct: 701  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXETFPPAKFLNNP 760

Query: 191  GLCGYPLPQCRVDSAANANSQHQRSH-RKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKK 250
            GLCGYPLP+C   S A+  + HQRSH R+ ASLAGSVAMGLLFS  CIFGLI+V  EM+K
Sbjct: 761  GLCGYPLPRCD-PSNADGYAHHQRSHGRRPASLAGSVAMGLLFSFVCIFGLILVGREMRK 820

Query: 251  RRKNKDAALDSYIDTHSQSGNTT--TVNWKNTCVR------------------------- 310
                       Y + H  SG+ T    NWK T V+                         
Sbjct: 821  XXXXXXXXXXMYAEGHGNSGDRTANNTNWKLTGVKEALSINLAAFEKPLRKLTFADLLQA 880

Query: 311  ------------------------------------------------------------ 370
                                                                        
Sbjct: 881  TNGFHNDSLIGSGGFGDVYKAILKDGSAVAIKKLIHVSGQGDREFMAEMETIGKIKHRNL 940

Query: 371  ------------------------------------------------------------ 392
                                                                        
Sbjct: 941  VPLLGYCKVGDERLLVYEFMKYGSLEDVLHDPKKAGVKLNWSTRRKIAIGSARGLAFLHH 1000

[Close]
BLAST of Carg05276 vs. TAIR10
Match: AT3G13380.1 (BRI1-like 3)

HSP 1 Score: 113.6 bits (283), Expect = 2.7e-25
Identity = 138/260 (53.08%), Postives = 177/260 (68.08%), Query Frame = 0 

Query: 22  ITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVYKGMTQPTFNHN 81
           ++GK +A+++N+G   C GAG LVEF GIR E++        C  TR+Y GMT   F+ N
Sbjct: 579 VSGKQFAFVRNEGGTDCRGAGGLVEFEGIRAERLEHFPMVHSCPKTRIYSGMTMYMFSSN 638

Query: 82  GSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 141
           GSMI+LDLS+N +S            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 639 GSMIYLDLSYNAVSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 698

Query: 142 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGLCGYPLPQCRVD 201
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX  Q  TFP + +ANNSGLCG PLP C   
Sbjct: 699 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGGQLTTFPLTRYANNSGLCGVPLPPC--- 758

Query: 202 SAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRKNKDAALDSYID 261
            ++ +      +H K+ S+A  ++ G++FS  CI  LI+ +   +K +K K+   + YI+
Sbjct: 759 -SSGSRPTRSHAHPKKQSIATGMSAGIVFSFMCIVMLIMALYRARKVQK-KEKQREKYIE 818

Query: 262 THSQSGNTTTVNWKNTCVRK 282
           +   SG++   +WK + V +
Sbjct: 819 SLPTSGSS---SWKLSSVHE 830


HSP 2 Score: 82.4 bits (202), Expect = 6.7e-16
Identity = 43/78 (55.13%), Postives = 56/78 (71.79%), Query Frame = 0 

Query: 281  KRPTDSADFG-DNNLVGWVKQ-HAKLDLTNVFDPELLKEDPNLKIELLEHLKVAVACLDD 340
            K+P D  +FG DNNLVGW KQ + +     + DPEL+  D +  +ELL +LK+A  CLDD
Sbjct: 1059 KKPIDPEEFGEDNNLVGWAKQLYREKRGAEILDPELV-TDKSGDVELLHYLKIASQCLDD 1118

Query: 341  RSWRRPTMIQVMTMFKEI 357
            R ++RPTMIQVMTMFKE+
Sbjct: 1119 RPFKRPTMIQVMTMFKEL 1135

[Close]
BLAST of Carg05276 vs. TAIR10
Match: AT1G55610.1 (BRI1 like)

HSP 1 Score: 101.3 bits (251), Expect = 1.4e-21
Identity = 128/203 (63.05%), Postives = 151/203 (74.38%), Query Frame = 0 

Query: 22  ITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVYKGMTQPTFNHN 81
           ++GK +A+++N+G   C GAG LVEF GIR E++ R+     C  TR+Y GMT  TF+ N
Sbjct: 579 VSGKQFAFVRNEGGTDCRGAGGLVEFEGIRAERLERLPMVHSCPATRIYSGMTMYTFSAN 638

Query: 82  GSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 141
           GSMI+ D+S+N +SG IP   G+   XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 639 GSMIYFDISYNAVSGFIPPGYGNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 698

Query: 142 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGLCGYPLPQCRVD 201
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Q  TFP S +ANNSGLCG PL  C   
Sbjct: 699 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGQLTTFPVSRYANNSGLCGVPLRPC--- 758

Query: 202 SAANANSQHQRSHRKQASLAGSV 225
            +A       R H K+ ++A +V
Sbjct: 759 GSAPRRPITSRIHAKKQTVATAV 778


HSP 2 Score: 81.6 bits (200), Expect = 1.1e-15
Identity = 49/110 (44.55%), Postives = 67/110 (60.91%), Query Frame = 0 

Query: 281  KRPTDSADFG-DNNLVGWVKQ-HAKLDLTNVFDPELLKEDPNLKIELLEHLKVAVACLDD 340
            K+P D  +FG DNNLVGW KQ + +     + DPEL+  D +  +EL  +LK+A  CLDD
Sbjct: 1061 KKPIDPGEFGEDNNLVGWAKQLYREKRGAEILDPELV-TDKSGDVELFHYLKIASQCLDD 1120

Query: 341  RSWRRPTMIQVMTMFKEIQAGSGMDSQSTIGSDNGGFSIDMVDMSLKEVP 389
            R ++RPTMIQ+M MFKE++A +  D            S+D  + SLKE P
Sbjct: 1121 RPFKRPTMIQLMAMFKEMKADTEEDE-----------SLD--EFSLKETP 1156

[Close]
BLAST of Carg05276 vs. TAIR10
Match: AT2G01950.1 (BRI1-like 2)

HSP 1 Score: 99.0 bits (245), Expect = 7.0e-21
Identity = 139/268 (51.87%), Postives = 177/268 (66.04%), Query Frame = 0 

Query: 13  QSGNIAVN-FITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVYK 72
           Q G+ A++  ++G + A+++N G+  C G G LVEF+GIR E++ +I S   C+FTR+Y 
Sbjct: 542 QPGSKALSGLLSGNTMAFVRNVGN-SCKGVGGLVEFSGIRPERLLQIPSLKSCDFTRMYS 601

Query: 73  GMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXXX 132
           G     F    ++ +LDLS+N L G IP EIG    XXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 602 GPILSLFTRYQTIEYLDLSYNQLRGKIPDEIGEMIXXXXXXXXXXXXXXXXXXXXXXXXX 661

Query: 133 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGLC 192
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Q  T PA+ +ANN GLC
Sbjct: 662 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGQLSTLPATQYANNPGLC 721

Query: 193 GYPLPQCRVDS----AANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKK 252
           G PLP+C+  +    A     +  +   + AS A S+ +G+L S   +  LI+  I ++ 
Sbjct: 722 GVPLPECKNGNNQLPAGTEEGKRAKHGTRAASWANSIVLGVLISAASVCILIVWAIAVRA 781

Query: 253 RRKNKDAALDSYIDTHSQSGNTTTVNWK 276
           RR++ D A       HS     +   WK
Sbjct: 782 RRRDADDAK----MLHSLQAVNSATTWK 804

[Close]
BLAST of Carg05276 vs. TAIR10
Match: AT3G08680.1 (Leucine-rich repeat protein kinase family protein)

HSP 1 Score: 49.3 bits (116), Expect = 6.3e-06
Identity = 32/93 (34.41%), Postives = 55/93 (59.14%), Query Frame = 0 

Query: 293 NLVGWVKQHAKLDLT-NVFDPELLKEDPNLKIELLEHLKVAVACLDDRSWRRPTMIQVMT 352
           +L  WV+   + + T  VFD EL+K+  N++ E+++ L++A+AC+      RP+M +V+ 
Sbjct: 550 DLPKWVQSVVREEWTGEVFDVELIKQQHNVEEEMVQMLQIAMACVSKHPDSRPSMEEVVN 609

Query: 353 MFKEIQ-AGSGMDSQSTIGSDNGGFSIDMVDMS 384
           M +EI+ +GSG       GS N   S +M+  S
Sbjct: 610 MMEEIRPSGSGP------GSGNRASSPEMIRSS 636

[Close]
BLAST of Carg05276 vs. Swiss-Prot
Match: sp|Q8GUQ5|BRI1_SOLLC (Brassinosteroid LRR receptor kinase OS=Solanum lycopersicum OX=4081 GN=CURL3 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 2.0e-81
Identity = 278/592 (46.96%), Postives = 309/592 (52.20%), Query Frame = 0 

Query: 14   SGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVYKGM 73
            SGNIAV  +TGK Y YIKNDGSK+CHGAGNL+EF GIRQEQ++RIS++ PCNFTRVY+G+
Sbjct: 595  SGNIAVALLTGKRYVYIKNDGSKECHGAGNLLEFGGIRQEQLDRISTRHPCNFTRVYRGI 654

Query: 74   TQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXXXXX 133
            TQPTFNHNGSMIFLDLS+N L GSIPKE+G+  Y  XXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 655  TQPTFNHNGSMIFLDLSYNKLEGSIPKELGAMYYLSXXXXXXXXXXXXXXXXXXXXXXXX 714

Query: 134  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGLCGY 193
            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX  F+TFP   FANNS LCGY
Sbjct: 715  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPFDTFPDYRFANNS-LCGY 774

Query: 194  PLP-QCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRKNK 253
            PLP  C     ++AN QHQ+SHR+QASLAGSVAMGLLFSLFCIFGLIIV IE KK     
Sbjct: 775  PLPIPCSSGPKSDAN-QHQKSHRRQASLAGSVAMGLLFSLFCIFGLIIVAIETKKXXXXX 834

Query: 254  DAALDSYIDTHSQSGNTTTVNWKNTCVR-------------------------------- 313
            +AAL++Y+D HS S  T    WK T  R                                
Sbjct: 835  EAALEAYMDGHSHSA-TANSAWKFTSAREALSINLAAFEKPLRKLTFADLLEATNGFHND 894

Query: 314  ------------------------------------------------------------ 373
                                                                        
Sbjct: 895  SLVGSGGFGDVYKAQLKDGSVVAIKKLIHVSGQGDREFTAEMETIGKIKHRNLVPLLGYC 954

Query: 374  ------------------------------------------------------------ 377
                                                                        
Sbjct: 955  KVGEERLLVYEYMKYGSLEDVLHDRKKIGIKLNWPARRKIAIGAARGLAFLHHNCIPHII 1014

[Close]
BLAST of Carg05276 vs. Swiss-Prot
Match: sp|Q8L899|BRI1_SOLPE (Systemin receptor SR160 OS=Solanum peruvianum OX=4082 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 2.0e-81
Identity = 278/592 (46.96%), Postives = 309/592 (52.20%), Query Frame = 0 

Query: 14   SGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVYKGM 73
            SGNIAV  +TGK Y YIKNDGSK+CHGAGNL+EF GIRQEQ++RIS++ PCNFTRVY+G+
Sbjct: 595  SGNIAVALLTGKRYVYIKNDGSKECHGAGNLLEFGGIRQEQLDRISTRHPCNFTRVYRGI 654

Query: 74   TQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXXXXX 133
            TQPTFNHNGSMIFLDLS+N L GSIPKE+G+  Y  XXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 655  TQPTFNHNGSMIFLDLSYNKLEGSIPKELGAMYYLSXXXXXXXXXXXXXXXXXXXXXXXX 714

Query: 134  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGLCGY 193
            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX  F+TFP   FANNS LCGY
Sbjct: 715  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPFDTFPDYRFANNS-LCGY 774

Query: 194  PLP-QCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRKNK 253
            PLP  C     ++AN QHQ+SHR+QASLAGSVAMGLLFSLFCIFGLIIV IE KK     
Sbjct: 775  PLPLPCSSGPKSDAN-QHQKSHRRQASLAGSVAMGLLFSLFCIFGLIIVAIETKKXXXXX 834

Query: 254  DAALDSYIDTHSQSGNTTTVNWKNTCVR-------------------------------- 313
            +AAL++Y+D HS S  T    WK T  R                                
Sbjct: 835  EAALEAYMDGHSHSA-TANSAWKFTSAREALSINLAAFEKPLRKLTFADLLEATNGFHND 894

Query: 314  ------------------------------------------------------------ 373
                                                                        
Sbjct: 895  SLVGSGGFGDVYKAQLKDGSVVAIKKLIHVSGQGDREFTAEMETIGKIKHRNLVPLLGYC 954

Query: 374  ------------------------------------------------------------ 377
                                                                        
Sbjct: 955  KVGEERLLVYEYMKYGSLEDVLHDRKKTGIKLNWPARRKIAIGAARGLAFLHHNCIPHII 1014

[Close]
BLAST of Carg05276 vs. Swiss-Prot
Match: sp|O22476|BRI1_ARATH (Protein BRASSINOSTEROID INSENSITIVE 1 OS=Arabidopsis thaliana OX=3702 GN=BRI1 PE=1 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.7e-80
Identity = 285/616 (46.27%), Postives = 320/616 (51.95%), Query Frame = 0 

Query: 11   FLQSGNIAVNFITGKSYAYIKNDG-SKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFT-R 70
            F QSG IA NFI GK Y YIKNDG  K+CHGAGNL+EF GIR EQ+NR+S+++PCN T R
Sbjct: 581  FKQSGKIAANFIAGKRYVYIKNDGMKKECHGAGNLLEFQGIRSEQLNRLSTRNPCNITSR 640

Query: 71   VYKGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXX 130
            VY G T PTF++NGSM+FLD+S+NMLSG IPKEIGS   XXXXXXXXXXXXXXXXXXXXX
Sbjct: 641  VYGGHTSPTFDNNGSMMFLDMSYNMLSGYIPKEIGSMXXXXXXXXXXXXXXXXXXXXXXX 700

Query: 131  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNS 190
            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX   ETFP + F NN 
Sbjct: 701  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXETFPPAKFLNNP 760

Query: 191  GLCGYPLPQCRVDSAANANSQHQRSH-RKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKK 250
            GLCGYPLP+C   S A+  + HQRSH R+ ASLAGSVAMGLLFS  CIFGLI+V  EM+K
Sbjct: 761  GLCGYPLPRCD-PSNADGYAHHQRSHGRRPASLAGSVAMGLLFSFVCIFGLILVGREMRK 820

Query: 251  RRKNKDAALDSYIDTHSQSGNTT--TVNWKNTCVR------------------------- 310
                       Y + H  SG+ T    NWK T V+                         
Sbjct: 821  XXXXXXXXXXMYAEGHGNSGDRTANNTNWKLTGVKEALSINLAAFEKPLRKLTFADLLQA 880

Query: 311  ------------------------------------------------------------ 370
                                                                        
Sbjct: 881  TNGFHNDSLIGSGGFGDVYKAILKDGSAVAIKKLIHVSGQGDREFMAEMETIGKIKHRNL 940

Query: 371  ------------------------------------------------------------ 392
                                                                        
Sbjct: 941  VPLLGYCKVGDERLLVYEFMKYGSLEDVLHDPKKAGVKLNWSTRRKIAIGSARGLAFLHH 1000

[Close]
BLAST of Carg05276 vs. Swiss-Prot
Match: sp|Q942F3|BRI1_ORYSJ (Brassinosteroid LRR receptor kinase BRI1 OS=Oryza sativa subsp. japonica OX=39947 GN=BRI1 PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 4.4e-49
Identity = 228/615 (37.07%), Postives = 283/615 (46.02%), Query Frame = 0 

Query: 13   QSGNIAVNFITGKSYAYIKNDG-SKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVYK 72
            QSG + V  I G+ Y Y++ND  S +C G G+L+EF  IR + ++R+ SK  CNFTR+Y 
Sbjct: 511  QSGKMNVGLIVGRPYVYLRNDELSSECRGKGSLLEFTSIRPDDLSRMPSKKLCNFTRMYV 570

Query: 73   GMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXXX 132
            G T+ TFN NGSMIFLDLS+N L  +IP E+G   Y  XXXXXXXXXXXXXXXXXXXXXX
Sbjct: 571  GSTEYTFNKNGSMIFLDLSYNQLDSAIPGELGDMFY-LXXXXXXXXXXXXXXXXXXXXXX 630

Query: 133  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGLC 192
            XXXXXXXXXXXXXXXXXXX    XXXXXXXXXXXXXXXXXXX             N+GLC
Sbjct: 631  XXXXXXXXXXXXXXXXXXXFSALXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNTGLC 690

Query: 193  GYPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRKN 252
            G+PLP C  D ++  +S   +SHR+QAS+A S+AMGLLFSLFCI      +   ++R KN
Sbjct: 691  GFPLPPC--DHSSPRSSNDHQSHRRQASMASSIAMGLLFSLFCIIXXXXAIGSKRRRLKN 750

Query: 253  KDAAL--DSYIDTHSQSGNTTT-------------------------------------- 312
            ++A+   D YID+ S S    +                                      
Sbjct: 751  EEASTSRDIYIDSRSHSATMNSDWRQNLSGTNLLSINLAAFEKPLQNLTLADLVEATNGF 810

Query: 313  ------------------------------------------------------------ 372
                                                                        
Sbjct: 811  HIACQIGSGGFGDVYKAQLKDGKVVAIKKLIHVSGQGDREFTAEMETIGKIKHRNLVPLL 870

Query: 373  ----------------------------------VNWK-----------------NTCV- 392
                                              +NW+                 + C+ 
Sbjct: 871  GYCKAGEERLLVYDYMKFGSLEDVLHDRKKIGKKLNWEARRKIAVGAARGLAFLHHNCIP 930

[Close]
BLAST of Carg05276 vs. Swiss-Prot
Match: sp|Q9LJF3|BRL3_ARATH (Receptor-like protein kinase BRI1-like 3 OS=Arabidopsis thaliana OX=3702 GN=BRL3 PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.9e-24
Identity = 138/260 (53.08%), Postives = 177/260 (68.08%), Query Frame = 0 

Query: 22  ITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVYKGMTQPTFNHN 81
           ++GK +A+++N+G   C GAG LVEF GIR E++        C  TR+Y GMT   F+ N
Sbjct: 579 VSGKQFAFVRNEGGTDCRGAGGLVEFEGIRAERLEHFPMVHSCPKTRIYSGMTMYMFSSN 638

Query: 82  GSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 141
           GSMI+LDLS+N +S            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 639 GSMIYLDLSYNAVSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 698

Query: 142 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGLCGYPLPQCRVD 201
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX  Q  TFP + +ANNSGLCG PLP C   
Sbjct: 699 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGGQLTTFPLTRYANNSGLCGVPLPPC--- 758

Query: 202 SAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRKNKDAALDSYID 261
            ++ +      +H K+ S+A  ++ G++FS  CI  LI+ +   +K +K K+   + YI+
Sbjct: 759 -SSGSRPTRSHAHPKKQSIATGMSAGIVFSFMCIVMLIMALYRARKVQK-KEKQREKYIE 818

Query: 262 THSQSGNTTTVNWKNTCVRK 282
           +   SG++   +WK + V +
Sbjct: 819 SLPTSGSS---SWKLSSVHE 830


HSP 2 Score: 82.4 bits (202), Expect = 1.2e-14
Identity = 43/78 (55.13%), Postives = 56/78 (71.79%), Query Frame = 0 

Query: 281  KRPTDSADFG-DNNLVGWVKQ-HAKLDLTNVFDPELLKEDPNLKIELLEHLKVAVACLDD 340
            K+P D  +FG DNNLVGW KQ + +     + DPEL+  D +  +ELL +LK+A  CLDD
Sbjct: 1059 KKPIDPEEFGEDNNLVGWAKQLYREKRGAEILDPELV-TDKSGDVELLHYLKIASQCLDD 1118

Query: 341  RSWRRPTMIQVMTMFKEI 357
            R ++RPTMIQVMTMFKE+
Sbjct: 1119 RPFKRPTMIQVMTMFKEL 1135

[Close]
BLAST of Carg05276 vs. TrEMBL
Match: tr|A0A1S3AZY8|A0A1S3AZY8_CUCME (protein BRASSINOSTEROID INSENSITIVE 1 OS=Cucumis melo OX=3656 GN=LOC103484679 PE=3 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 5.7e-118
Identity = 338/607 (55.68%), Postives = 349/607 (57.50%), Query Frame = 0 

Query: 11   FLQSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVY 70
            F QSGNIAVNFITGKSYAYIKNDGSKQCHGAGNL+EFAGIRQEQV+RISSKSPCNFTRVY
Sbjct: 594  FRQSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLLEFAGIRQEQVSRISSKSPCNFTRVY 653

Query: 71   KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXX 130
            KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGST  XXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 654  KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTXXXXXXXXXXXXXXXXXXXXXXXXX 713

Query: 131  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL 190
            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL
Sbjct: 714  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL 773

Query: 191  CGYPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRK 250
            CGYPLP C VDSA NANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLI           
Sbjct: 774  CGYPLPPCVVDSAGNANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIXXXXXXXXXXX 833

Query: 251  NKDAALDSYIDTHSQSGNTTTVNWKNTCVR------------------------------ 310
               + LDSY+++HSQSG TT VNWK T  R                              
Sbjct: 834  XXXSTLDSYVESHSQSGTTTAVNWKLTGAREALSINLATFEKPLRKLTFADLLEATNGFH 893

Query: 311  ------------------------------------------------------------ 370
                                                                        
Sbjct: 894  NDSMIGSGGFGDVYKAQLKDGSTVAIKKLIHVSGQGDREFTAEMETIGKIKHRNLVPLLG 953

Query: 371  ------------------------------------------------------------ 390
                                                                        
Sbjct: 954  YCKVGEERLLVYEYMKYGSLEDVLHDQKKGGIKLNWSARRKIAIGAARGLAFLHHNCIPH 1013

[Close]
BLAST of Carg05276 vs. TrEMBL
Match: tr|A0A0A0KHY5|A0A0A0KHY5_CUCSA (Brassinosteroid insensitive 1 protein OS=Cucumis sativus OX=3659 GN=bri1 PE=2 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 6.5e-114
Identity = 332/607 (54.70%), Postives = 343/607 (56.51%), Query Frame = 0 

Query: 11   FLQSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVY 70
            F QSGNIAVNFITGKSYAYIKNDGSKQCHGAGNL+EFAGIRQEQVNRISSKSPCNFTRVY
Sbjct: 588  FRQSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLLEFAGIRQEQVNRISSKSPCNFTRVY 647

Query: 71   KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXX 130
            KGM QPTFNHNGSMIFLDLSHNML+GSIPK       XXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 648  KGMIQPTFNHNGSMIFLDLSHNMLTGSIPKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 707

Query: 131  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL 190
            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL
Sbjct: 708  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL 767

Query: 191  CGYPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRK 250
            CGYPLP C VDSA NANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGL            
Sbjct: 768  CGYPLPPCVVDSAGNANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLXXXXXXXXXXXX 827

Query: 251  NKDAALDSYIDTHSQSGNTTTVNWKNTCVR------------------------------ 310
               +ALDSY+++HSQSG TT VNWK T  R                              
Sbjct: 828  XXXSALDSYVESHSQSGTTTAVNWKLTGAREALSINLATFEKPLRKLTFADLLEATNGFH 887

Query: 311  ------------------------------------------------------------ 370
                                                                        
Sbjct: 888  NDSLIGSGGFGDVYKAQLKDGSTVAIKKLIHVSGQGDREFTAEMETIGKIKHRNLVPLLG 947

Query: 371  ------------------------------------------------------------ 390
                                                                        
Sbjct: 948  YCKVGEERLLVYEYMKYGSLEDVLHDQKKGGIKLNWSARRKIAIGAARGLAFLHHNCIPH 1007

[Close]
BLAST of Carg05276 vs. TrEMBL
Match: tr|A0A2N9G2W6|A0A2N9G2W6_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS21551 PE=3 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.4e-100
Identity = 301/610 (49.34%), Postives = 338/610 (55.41%), Query Frame = 0 

Query: 11   FLQSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVY 70
            F QSG+I V +++ K+Y YIKNDGSK+CHGAGNL+EFAGIR EQ+NRIS+++PCNFTRVY
Sbjct: 579  FKQSGSIGVKYLSQKTYVYIKNDGSKECHGAGNLLEFAGIRHEQLNRISTRNPCNFTRVY 638

Query: 71   KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXX 130
             G  QPTF HNGSMIFLD+SHNMLSGSIPKEIGST Y XXXXXXXXXXXXXXXXXXXXXX
Sbjct: 639  AGKLQPTFYHNGSMIFLDISHNMLSGSIPKEIGSTMYLXXXXXXXXXXXXXXXXXXXXXX 698

Query: 131  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL 190
            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX  QFETF AS F NNSGL
Sbjct: 699  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMGQFETFQASSFQNNSGL 758

Query: 191  CGYPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRK 250
            CGYPLP+C  D ++++ SQHQ+SHR+QASLAGSVAMGLLFSLFCIFGLIIV IE KKRRK
Sbjct: 759  CGYPLPKCGEDPSSSSGSQHQKSHRRQASLAGSVAMGLLFSLFCIFGLIIVAIETKKRRK 818

Query: 251  NKDAALDSYIDTHSQSGNTTTVNWKNTCVR------------------------------ 310
             KD A D Y+D+ S SG T  V+W  T  R                              
Sbjct: 819  KKDVAFDGYVDSRSHSG-TANVSWMPTGTREALSINLATFENLLRRLTFADLVEATNNFN 878

Query: 311  ------------------------------------------------------------ 370
                                                                        
Sbjct: 879  SSSLIGSGGFGDVYKAQLKDGSIVAIKKLIHISGQGDREFTAEMETIGKLNHRNLVPLLG 938

Query: 371  ------------------------------------------------------------ 392
                                                                        
Sbjct: 939  YCKSKEERLLVYEYMRYGSLEDVLHDQKKAGIKLNWAARRKIAIGAARGLAYLHHSCNPH 998

[Close]
BLAST of Carg05276 vs. TrEMBL
Match: tr|A0A251NRJ3|A0A251NRJ3_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G168100 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 5.0e-98
Identity = 303/608 (49.84%), Postives = 340/608 (55.92%), Query Frame = 0 

Query: 11   FLQSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVY 70
            F QSGNIAVNFI  K+YAYIKNDGSK+CHGAGNL+EFAGIR E +NRIS+++PCNFTRVY
Sbjct: 596  FKQSGNIAVNFIVSKTYAYIKNDGSKECHGAGNLLEFAGIRDEHLNRISARNPCNFTRVY 655

Query: 71   KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXX 130
            +GM QPTFNHNGSMIFLDLSHN+LSGSIPKEIG   YXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 656  RGMIQPTFNHNGSMIFLDLSHNLLSGSIPKEIGKMYYXXXXXXXXXXXXXXXXXXXXXXX 715

Query: 131  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL 190
            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX QFETFPA  F NNSGL
Sbjct: 716  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGQFETFPAYRFINNSGL 775

Query: 191  CGYPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRK 250
            CGYPL  C   S  NAN+ HQ+SHR+QASL GSVAMGLLFSLFCIFGL+IV IE KKRRK
Sbjct: 776  CGYPLSPCGGASGPNANA-HQKSHRRQASLVGSVAMGLLFSLFCIFGLLIVAIETKKRRK 835

Query: 251  NKDAALDSYIDTHSQSGNTT---------------------------------------- 310
             KD+ALD YID+ +QSG                                           
Sbjct: 836  KKDSALDVYIDSRNQSGTVNGWKLPGTKEALSINLATFEKPLQKLTFADLLEATNGFHDD 895

Query: 311  ------------------------------------------------------------ 370
                                                                        
Sbjct: 896  SLIGSGGFGDVYKAKLKDGSIVAIKKLIHISGQGDREFTAEMETIGKIKHRNLVPLLGYC 955

Query: 371  ------------------------------TVNW-----------------KNTCV---- 392
                                           +NW                  + C+    
Sbjct: 956  KVGEERLLVYEYMKYGSLDDVLHEPKKAGIKLNWAARRKIAIGSARGLAFLHHNCIPHII 1015

[Close]
BLAST of Carg05276 vs. TrEMBL
Match: tr|M5W8C2|M5W8C2_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa000566mg PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 5.0e-98
Identity = 303/608 (49.84%), Postives = 340/608 (55.92%), Query Frame = 0 

Query: 11   FLQSGNIAVNFITGKSYAYIKNDGSKQCHGAGNLVEFAGIRQEQVNRISSKSPCNFTRVY 70
            F QSGNIAVNFI  K+YAYIKNDGSK+CHGAGNL+EFAGIR E +NRIS+++PCNFTRVY
Sbjct: 488  FKQSGNIAVNFIVSKTYAYIKNDGSKECHGAGNLLEFAGIRDEHLNRISARNPCNFTRVY 547

Query: 71   KGMTQPTFNHNGSMIFLDLSHNMLSGSIPKEIGSTKYXXXXXXXXXXXXXXXXXXXXXXX 130
            +GM QPTFNHNGSMIFLDLSHN+LSGSIPKEIG   YXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 548  RGMIQPTFNHNGSMIFLDLSHNLLSGSIPKEIGKMYYXXXXXXXXXXXXXXXXXXXXXXX 607

Query: 131  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAQFETFPASGFANNSGL 190
            XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX QFETFPA  F NNSGL
Sbjct: 608  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGQFETFPAYRFINNSGL 667

Query: 191  CGYPLPQCRVDSAANANSQHQRSHRKQASLAGSVAMGLLFSLFCIFGLIIVVIEMKKRRK 250
            CGYPL  C   S  NAN+ HQ+SHR+QASL GSVAMGLLFSLFCIFGL+IV IE KKRRK
Sbjct: 668  CGYPLSPCGGASGPNANA-HQKSHRRQASLVGSVAMGLLFSLFCIFGLLIVAIETKKRRK 727

Query: 251  NKDAALDSYIDTHSQSGNTT---------------------------------------- 310
             KD+ALD YID+ +QSG                                           
Sbjct: 728  KKDSALDVYIDSRNQSGTVNGWKLPGTKEALSINLATFEKPLQKLTFADLLEATNGFHDD 787

Query: 311  ------------------------------------------------------------ 370
                                                                        
Sbjct: 788  SLIGSGGFGDVYKAKLKDGSIVAIKKLIHISGQGDREFTAEMETIGKIKHRNLVPLLGYC 847

Query: 371  ------------------------------TVNW-----------------KNTCV---- 392
                                           +NW                  + C+    
Sbjct: 848  KVGEERLLVYEYMKYGSLDDVLHEPKKAGIKLNWAARRKIAIGSARGLAFLHHNCIPHII 907

The following BLAST results are available for this feature:
BLAST of Carg05276 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023518235.12.4e-14462.46protein BRASSINOSTEROID INSENSITIVE 1 [Cucurbita pepo subsp. pepo][more]
XP_022963139.11.6e-14362.34protein BRASSINOSTEROID INSENSITIVE 1 [Cucurbita moschata][more]
XP_023003428.12.2e-14261.80protein BRASSINOSTEROID INSENSITIVE 1 [Cucurbita maxima][more]
XP_008440121.18.6e-11855.68PREDICTED: protein BRASSINOSTEROID INSENSITIVE 1 [Cucumis melo][more]
XP_023544828.14.3e-11755.78systemin receptor SR160-like [Cucurbita pepo subsp. pepo][more]
BLAST of Carg05276 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G39400.19.2e-8246.27Leucine-rich receptor-like protein kinase family protein[more]
AT3G13380.12.7e-2553.08BRI1-like 3[more]
AT1G55610.11.4e-2163.05BRI1 like[more]
AT2G01950.17.0e-2151.87BRI1-like 2[more]
AT3G08680.16.3e-0634.41Leucine-rich repeat protein kinase family protein[more]
BLAST of Carg05276 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q8GUQ5|BRI1_SOLLC2.0e-8146.96Brassinosteroid LRR receptor kinase OS=Solanum lycopersicum OX=4081 GN=CURL3 PE=... [more]
sp|Q8L899|BRI1_SOLPE2.0e-8146.96Systemin receptor SR160 OS=Solanum peruvianum OX=4082 PE=1 SV=1[more]
sp|O22476|BRI1_ARATH1.7e-8046.27Protein BRASSINOSTEROID INSENSITIVE 1 OS=Arabidopsis thaliana OX=3702 GN=BRI1 PE... [more]
sp|Q942F3|BRI1_ORYSJ4.4e-4937.07Brassinosteroid LRR receptor kinase BRI1 OS=Oryza sativa subsp. japonica OX=3994... [more]
sp|Q9LJF3|BRL3_ARATH4.9e-2453.08Receptor-like protein kinase BRI1-like 3 OS=Arabidopsis thaliana OX=3702 GN=BRL3... [more]
BLAST of Carg05276 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3AZY8|A0A1S3AZY8_CUCME5.7e-11855.68protein BRASSINOSTEROID INSENSITIVE 1 OS=Cucumis melo OX=3656 GN=LOC103484679 PE... [more]
tr|A0A0A0KHY5|A0A0A0KHY5_CUCSA6.5e-11454.70Brassinosteroid insensitive 1 protein OS=Cucumis sativus OX=3659 GN=bri1 PE=2 SV... [more]
tr|A0A2N9G2W6|A0A2N9G2W6_FAGSY1.4e-10049.34Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS21551 PE=3 SV=1[more]
tr|A0A251NRJ3|A0A251NRJ3_PRUPE5.0e-9849.84Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G168100 PE=3 SV=1[more]
tr|M5W8C2|M5W8C2_PRUPE5.0e-9849.84Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa000566mg PE=4 SV=1[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
Vocabulary: INTERPRO
TermDefinition
IPR032675LRR_dom_sf
IPR001611Leu-rich_rpt
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05276-RACarg05276-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00019LEURICHRPTcoord: 129..142
score: 54.32
coord: 84..97
score: 46.68
NoneNo IPR availableGENE3DG3DSA:3.30.1490.310coord: 23..83
e-value: 9.8E-88
score: 296.9
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 274..358
e-value: 3.5E-10
score: 41.6
NoneNo IPR availablePANTHERPTHR27000FAMILY NOT NAMEDcoord: 1..273
coord: 281..390
NoneNo IPR availablePANTHERPTHR27000:SF220PROTEIN BRASSINOSTEROID INSENSITIVE 1coord: 1..273
coord: 281..390
NoneNo IPR availableSUPERFAMILYSSF52058L domain-likecoord: 72..180
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 108..166
e-value: 3.4E-8
score: 33.0
IPR032675Leucine-rich repeat domain superfamilyGENE3DG3DSA:3.80.10.10coord: 2..22
e-value: 9.8E-88
score: 296.9
coord: 84..198
e-value: 9.8E-88
score: 296.9