Carg05274 (gene) Silver-seed gourd

NameCarg05274
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionGlucan endo-1,3-beta-glucosidase-like protein
LocationCucurbita_argyrosperma_scaffold_010 : 846537 .. 848718 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TAATCAGTTTAAACAAGAACCAAGTTCATGTTCCTGTGTCCCCCTCCTAAAGAGGAGGAACTCTGTTCATTTTGACCCTTTCATCTTCCAATACAACCCTGAAATCACAGCTTTCTCTATATATAATCCAAATCATTCCCCAAATTAATCACAACCCAACTCCCAAAAACCATCCATTTTCACTCTCACCCATTACAAGAGATTGACCCTATGGGCGGAGCAACGATCGTCCCCACCGCGTGGGCCGCCGTGATTACCGTGGCGGCGATGGCGGCTGGCGTTGCAGAGGGAGGAATCGGCGTAAACTGGGGAACATTGGCATCGCACCCTCTGAAACCGAGCATTGTGGTGAACCTGCTGAAGGATAATGGAATTAAAAAAGTGAAGCTCTTCGATTCCGACACTTGGACTGTATCATCGCTGGCTGGTTCTAAAATTGAAACCATTATTGGCATTCCTAATGATCGCCTTAAGAAATTTGCTGAAAGCTACAACGAAGCTAAGAATTGGGTTAAAGATAATGTTACCGTTCATATGTACGATGGCGGCGTCGATATCAGGTTCCTTCCTCTTATTCTAATCTTTTTTTTTTAGTAATATCACAATTTAATCCGAAAAATCTCGTAAACATAAAAGATTTCTGTTCGATAACTAAAAAAACTTACATAAAATTATATTCCCGTCCTCTCTCACTTTTTATATAGTAATATTAATTTAATAAAAATGGTATTGAAAAATAGAAAGTTAAAGTCTCTAATATTATATATATATATATATATATAAACAAGTATTTATAAGGCTATGAAAATTATAATTATTTAATTAATTTTAAAAAATTAAGTACAAAATTAAAATAGTAGGTATATATATTTGAATAATAATAATTTTTATACAATAAAAGAGAAATTTAAATATATTTTTAAAATTTATGAGAGTAAAAACTAAAATATCTCGAAAATATAATGATAAATTATAAATTTTATTAAAAAATATATAAACTATAATTGGATTAAAAAAAATAAAATGTTTATTGGAAAAATATGAGAAAAGAAATGTAAGAATTGTGTGGTAAAAGTGCAGGTATGTGTCTGTGGGAAATGAAGCTTTCTTGACGGCTTACAATGGATCCTTTGTGAACGTCACGTTTCCGGCGATGCAAAACGTACAGAAAGCTCTCGACGAAGCCGGCCATAGCAAAAAAATCAAGGTCACCACTGCCCTCAACGCCGACGTCTACGAAGCCAAGTCCAATCTACCGTCCGACGGCCAATTCCGACCAGACATTTACAGTATCATGAAGGAGATAGTCCGCTTCCTCGACCGTCACGATGCCCCTTTCATGGTCAACATTTACCCTTTCCTCAGTCTTTACCAAAACCCTAATTTCCCCATCGATTACGCCTTCTTCGGCGGCAGCGGACAGTCCAACAATGACAATGGCAAATCCTACACCAACGTCTTCGATGCCAATTACGACACGCTGATTTGGTCCTTGAAGAAAGTAGGCTTCCCCAACCTGAAAATCGTCATCGGAGAAGTCGGTTGGCCGACGGACGCCAACGTCTTCGCGAATGTAACTCTGGCGAAACGATTCTACGACGGCTTGTTCGAGAAATTGGCCTCTGGAGAAGGAACTCCAATGAGGCCGAAGGAGAGCTTCGAGGTGTACTTGTTCGGGCTTTTGGACGAGAACGCAAAAAGCGTTTTGCCAGGTTTTTTCGAGCGCCATTGGGGGATTTTTCAATTCGATGGAAAACCTAAATTTCCAATGGATTTATCGGGGAAAGGAAACGACAAAATGCTGGTGGCGGCGAAGGGAGTGCAGTATATGGAGCGAAAATGGTGCGTTTTGAGGCAGACTTTGAAGAATTTGGAGCCAACTGCGAAGGAAGTGGATTACGCTTGCGGTTACTCCGACTGTACGGCCTTGACAGCCGGAGGATCGTGCGGTCAATTGGACCGGCGAGGCAGAATTTCGTATGCGTTTAACATATATTTTCAGATGCAGGATCAGAACGTGGAGGCCTGTGTTTTCGATGGAACCGCCGAGATTGTGACCAAAAATGCGACGGTTGGGAACTGCTTGTTTCCGATCCAGATTGTGAGCGGCGGAGAGAGGTTGAAGGCGGCGGCGGCGGCCATGGTTGGGCTTGTGTTGAGCTTGTTTGTGTTAGGGCTGTGA

mRNA sequence

TAATCAGTTTAAACAAGAACCAAGTTCATGTTCCTGTGTCCCCCTCCTAAAGAGGAGGAACTCTGTTCATTTTGACCCTTTCATCTTCCAATACAACCCTGAAATCACAGCTTTCTCTATATATAATCCAAATCATTCCCCAAATTAATCACAACCCAACTCCCAAAAACCATCCATTTTCACTCTCACCCATTACAAGAGATTGACCCTATGGGCGGAGCAACGATCGTCCCCACCGCGTGGGCCGCCGTGATTACCGTGGCGGCGATGGCGGCTGGCGTTGCAGAGGGAGGAATCGGCGTAAACTGGGGAACATTGGCATCGCACCCTCTGAAACCGAGCATTGTGGTGAACCTGCTGAAGGATAATGGAATTAAAAAAGTGAAGCTCTTCGATTCCGACACTTGGACTGTATCATCGCTGGCTGGTTCTAAAATTGAAACCATTATTGGCATTCCTAATGATCGCCTTAAGAAATTTGCTGAAAGCTACAACGAAGCTAAGAATTGGGTTAAAGATAATGTTACCGTTCATATGTACGATGGCGGCGTCGATATCAGGTATGTGTCTGTGGGAAATGAAGCTTTCTTGACGGCTTACAATGGATCCTTTGTGAACGTCACGTTTCCGGCGATGCAAAACGTACAGAAAGCTCTCGACGAAGCCGGCCATAGCAAAAAAATCAAGGTCACCACTGCCCTCAACGCCGACGTCTACGAAGCCAAGTCCAATCTACCGTCCGACGGCCAATTCCGACCAGACATTTACAGTATCATGAAGGAGATAGTCCGCTTCCTCGACCGTCACGATGCCCCTTTCATGGTCAACATTTACCCTTTCCTCAGTCTTTACCAAAACCCTAATTTCCCCATCGATTACGCCTTCTTCGGCGGCAGCGGACAGTCCAACAATGACAATGGCAAATCCTACACCAACGTCTTCGATGCCAATTACGACACGCTGATTTGGTCCTTGAAGAAAGTAGGCTTCCCCAACCTGAAAATCGTCATCGGAGAAGTCGGTTGGCCGACGGACGCCAACGTCTTCGCGAATGTAACTCTGGCGAAACGATTCTACGACGGCTTGTTCGAGAAATTGGCCTCTGGAGAAGGAACTCCAATGAGGCCGAAGGAGAGCTTCGAGGTGTACTTGTTCGGGCTTTTGGACGAGAACGCAAAAAGCGTTTTGCCAGGTTTTTTCGAGCGCCATTGGGGGATTTTTCAATTCGATGGAAAACCTAAATTTCCAATGGATTTATCGGGGAAAGGAAACGACAAAATGCTGGTGGCGGCGAAGGGAGTGCAGTATATGGAGCGAAAATGGTGCGTTTTGAGGCAGACTTTGAAGAATTTGGAGCCAACTGCGAAGGAAGTGGATTACGCTTGCGGTTACTCCGACTGTACGGCCTTGACAGCCGGAGGATCGTGCGGTCAATTGGACCGGCGAGGCAGAATTTCGTATGCGTTTAACATATATTTTCAGATGCAGGATCAGAACGTGGAGGCCTGTGTTTTCGATGGAACCGCCGAGATTGTGACCAAAAATGCGACGGTTGGGAACTGCTTGTTTCCGATCCAGATTGTGAGCGGCGGAGAGAGGTTGAAGGCGGCGGCGGCGGCCATGGTTGGGCTTGTGTTGAGCTTGTTTGTGTTAGGGCTGTGA

Coding sequence (CDS)

ATGGGCGGAGCAACGATCGTCCCCACCGCGTGGGCCGCCGTGATTACCGTGGCGGCGATGGCGGCTGGCGTTGCAGAGGGAGGAATCGGCGTAAACTGGGGAACATTGGCATCGCACCCTCTGAAACCGAGCATTGTGGTGAACCTGCTGAAGGATAATGGAATTAAAAAAGTGAAGCTCTTCGATTCCGACACTTGGACTGTATCATCGCTGGCTGGTTCTAAAATTGAAACCATTATTGGCATTCCTAATGATCGCCTTAAGAAATTTGCTGAAAGCTACAACGAAGCTAAGAATTGGGTTAAAGATAATGTTACCGTTCATATGTACGATGGCGGCGTCGATATCAGGTATGTGTCTGTGGGAAATGAAGCTTTCTTGACGGCTTACAATGGATCCTTTGTGAACGTCACGTTTCCGGCGATGCAAAACGTACAGAAAGCTCTCGACGAAGCCGGCCATAGCAAAAAAATCAAGGTCACCACTGCCCTCAACGCCGACGTCTACGAAGCCAAGTCCAATCTACCGTCCGACGGCCAATTCCGACCAGACATTTACAGTATCATGAAGGAGATAGTCCGCTTCCTCGACCGTCACGATGCCCCTTTCATGGTCAACATTTACCCTTTCCTCAGTCTTTACCAAAACCCTAATTTCCCCATCGATTACGCCTTCTTCGGCGGCAGCGGACAGTCCAACAATGACAATGGCAAATCCTACACCAACGTCTTCGATGCCAATTACGACACGCTGATTTGGTCCTTGAAGAAAGTAGGCTTCCCCAACCTGAAAATCGTCATCGGAGAAGTCGGTTGGCCGACGGACGCCAACGTCTTCGCGAATGTAACTCTGGCGAAACGATTCTACGACGGCTTGTTCGAGAAATTGGCCTCTGGAGAAGGAACTCCAATGAGGCCGAAGGAGAGCTTCGAGGTGTACTTGTTCGGGCTTTTGGACGAGAACGCAAAAAGCGTTTTGCCAGGTTTTTTCGAGCGCCATTGGGGGATTTTTCAATTCGATGGAAAACCTAAATTTCCAATGGATTTATCGGGGAAAGGAAACGACAAAATGCTGGTGGCGGCGAAGGGAGTGCAGTATATGGAGCGAAAATGGTGCGTTTTGAGGCAGACTTTGAAGAATTTGGAGCCAACTGCGAAGGAAGTGGATTACGCTTGCGGTTACTCCGACTGTACGGCCTTGACAGCCGGAGGATCGTGCGGTCAATTGGACCGGCGAGGCAGAATTTCGTATGCGTTTAACATATATTTTCAGATGCAGGATCAGAACGTGGAGGCCTGTGTTTTCGATGGAACCGCCGAGATTGTGACCAAAAATGCGACGGTTGGGAACTGCTTGTTTCCGATCCAGATTGTGAGCGGCGGAGAGAGGTTGAAGGCGGCGGCGGCGGCCATGGTTGGGCTTGTGTTGAGCTTGTTTGTGTTAGGGCTGTGA

Protein sequence

MGGATIVPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKLFDSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVSVGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQFRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSYTNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGEGTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVAAKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFNIYFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLKAAAAAMVGLVLSLFVLGL
BLAST of Carg05274 vs. NCBI nr
Match: XP_022963052.1 (glucan endo-1,3-beta-glucosidase 8-like [Cucurbita moschata])

HSP 1 Score: 979.9 bits (2532), Expect = 3.0e-282
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MGGATIVPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL 60
           MGGATIVPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL
Sbjct: 1   MGGATIVPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL 60

Query: 61  FDSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVS 120
           FDSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVS
Sbjct: 61  FDSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVS 120

Query: 121 VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQ 180
           VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQ
Sbjct: 121 VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQ 180

Query: 181 FRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSY 240
           FRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSY
Sbjct: 181 FRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSY 240

Query: 241 TNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE 300
           TNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE
Sbjct: 241 TNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE 300

Query: 301 GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA 360
           GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA
Sbjct: 301 GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA 360

Query: 361 AKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFN 420
           AKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFN
Sbjct: 361 AKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFN 420

Query: 421 IYFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLKAAAAAMVGLVLSLFV 480
           IYFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLKAAAAAMVGLVLSLFV
Sbjct: 421 IYFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLKAAAAAMVGLVLSLFV 480

Query: 481 LGL 484
           LGL
Sbjct: 481 LGL 483

BLAST of Carg05274 vs. NCBI nr
Match: XP_023003994.1 (glucan endo-1,3-beta-glucosidase 8-like [Cucurbita maxima])

HSP 1 Score: 956.1 bits (2470), Expect = 4.7e-275
Identity = 466/483 (96.48%), Postives = 475/483 (98.34%), Query Frame = 0

Query: 1   MGGATIVPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL 60
           MGGA IVPT WAAVITVAAMAAGVAEGG+GVNWGTLASHPLKPSIVVNLLKDNGIKKVKL
Sbjct: 1   MGGAAIVPTVWAAVITVAAMAAGVAEGGVGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL 60

Query: 61  FDSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVS 120
           FDSDTWTVSSLAGSKIET+IGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGG+DIRYVS
Sbjct: 61  FDSDTWTVSSLAGSKIETVIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGIDIRYVS 120

Query: 121 VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQ 180
           VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGH KKIKVTTALNADVYEAKSNLPSDGQ
Sbjct: 121 VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHGKKIKVTTALNADVYEAKSNLPSDGQ 180

Query: 181 FRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSY 240
           FRPDIYSI+KEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSN+DNGKSY
Sbjct: 181 FRPDIYSIIKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNDDNGKSY 240

Query: 241 TNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE 300
           TNVFDANYDTLIWSLKK+GFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE
Sbjct: 241 TNVFDANYDTLIWSLKKIGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE 300

Query: 301 GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA 360
           GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA
Sbjct: 301 GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA 360

Query: 361 AKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFN 420
           AKGVQYMERKWCVLRQTLKNL+PTA+EVDYAC YSDCTALTAGGSC QLDRRGRISYAFN
Sbjct: 361 AKGVQYMERKWCVLRQTLKNLDPTAREVDYACSYSDCTALTAGGSCSQLDRRGRISYAFN 420

Query: 421 IYFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLKAAAAAMVGLVLSLFV 480
           IYFQMQDQNVEACVF GTAEIVTKNAT+GNCLFPIQIVS GERLKAAA AMVGLVLSLFV
Sbjct: 421 IYFQMQDQNVEACVFGGTAEIVTKNATIGNCLFPIQIVSAGERLKAAAVAMVGLVLSLFV 480

Query: 481 LGL 484
           LGL
Sbjct: 481 LGL 483

BLAST of Carg05274 vs. NCBI nr
Match: XP_023518363.1 (glucan endo-1,3-beta-glucosidase 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 956.1 bits (2470), Expect = 4.7e-275
Identity = 473/486 (97.33%), Postives = 476/486 (97.94%), Query Frame = 0

Query: 1   MGGATIVPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL 60
           MGGATI PT WAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL
Sbjct: 1   MGGATIFPTTWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL 60

Query: 61  FDSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVS 120
           FDSD WTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVK+NVTVHMYDGGVDIRYVS
Sbjct: 61  FDSDPWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKENVTVHMYDGGVDIRYVS 120

Query: 121 VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQ 180
           VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQ
Sbjct: 121 VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQ 180

Query: 181 FRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSY 240
           FRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQ+NNDNGKSY
Sbjct: 181 FRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQANNDNGKSY 240

Query: 241 TNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE 300
           TNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE
Sbjct: 241 TNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE 300

Query: 301 GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA 360
           GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA
Sbjct: 301 GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA 360

Query: 361 AKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFN 420
           AKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSC QLDRRGRISYAFN
Sbjct: 361 AKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCSQLDRRGRISYAFN 420

Query: 421 IYFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLKAAA---AAMVGLVLS 480
           IYFQ+QDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVS GERLKAAA     MVGLVLS
Sbjct: 421 IYFQIQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSAGERLKAAAXXXXXMVGLVLS 480

Query: 481 LFVLGL 484
           LFVLGL
Sbjct: 481 LFVLGL 486

BLAST of Carg05274 vs. NCBI nr
Match: XP_008440124.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 8 [Cucumis melo])

HSP 1 Score: 793.5 bits (2048), Expect = 4.0e-226
Identity = 375/484 (77.48%), Postives = 432/484 (89.26%), Query Frame = 0

Query: 1   MGGATIVPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL 60
           M G +IVP  WAAV+TVAA+ + V   GIG NWG ++SHPL+P+IVV LLKDNGIKKVKL
Sbjct: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60

Query: 61  FDSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVS 120
           FDSD+WTVS+L+GSKIET+IGIPND+LKKFAESY++AK+WVK+NVT HM++GGVD+RYVS
Sbjct: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120

Query: 121 VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQ 180
           VGNEAFL+AYNG++V VT+PAMQN+QKALD AGH KK+KVTTALNADVYE+ +NLPSDGQ
Sbjct: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180

Query: 181 FRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSY 240
           FRPDIY  MK+IV FLDR+ APFMVNIYPFLSLYQNPNFP+DYAFFGG  ++ ND GKSY
Sbjct: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240

Query: 241 TNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE 300
           TNVFDANYDTLIWSLKK+G  ++KI++GEVGWPTD N FANV LAKRFYDGLF+KLASG 
Sbjct: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300

Query: 301 GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA 360
           GTPMRPKE FEVYLFGLLDE+ KSVLPGFFERHWGIFQFDGKPKFPMD+SGKGNDKMLVA
Sbjct: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360

Query: 361 AKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFN 420
           AKGVQY+E+KWCVL++ +KN +  +KEVDYACG SDCT+L  G SC  LD+RG ISYAFN
Sbjct: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420

Query: 421 IYFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLK-AAAAAMVGLVLSLF 480
           +Y+QMQDQ+VEAC F G+A IV KNA+VG+CLFPIQIVS GERLK AAAAA+VGLVL LF
Sbjct: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480

Query: 481 VLGL 484
            LGL
Sbjct: 481 ALGL 484

BLAST of Carg05274 vs. NCBI nr
Match: XP_004142041.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 8 [Cucumis sativus] >KGN48417.1 hypothetical protein Csa_6G486900 [Cucumis sativus])

HSP 1 Score: 792.0 bits (2044), Expect = 1.2e-225
Identity = 377/483 (78.05%), Postives = 430/483 (89.03%), Query Frame = 0

Query: 1   MGGATIVPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL 60
           M G ++VP  WAAV+T+ A+ + V   GIGVNWG L+SHPL+P+IVV LLKDNGIKKVKL
Sbjct: 1   MAGVSMVPAVWAAVMTLGAVFSVVEGAGIGVNWGILSSHPLRPNIVVKLLKDNGIKKVKL 60

Query: 61  FDSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVS 120
           FDSD WTVSSL+GSKIETIIGIPND+LKKFA+SY++AK+WVK+NVT HM++GGVD+RYVS
Sbjct: 61  FDSDAWTVSSLSGSKIETIIGIPNDQLKKFAKSYDDAKDWVKENVTSHMFEGGVDLRYVS 120

Query: 121 VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQ 180
           VGNEAFLTAYNGSFV VTFPAMQN+QKA+D AGH KKIKVTTALNADVYE+ +NLPSDG+
Sbjct: 121 VGNEAFLTAYNGSFVKVTFPAMQNIQKAIDAAGHGKKIKVTTALNADVYESSTNLPSDGE 180

Query: 181 FRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSY 240
           FRPDIYS MK+IV FLDR+ APFMVNIYPFLSLYQNPNFP+DYAFF G G++ ND  KSY
Sbjct: 181 FRPDIYSTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFDGGGKATNDKDKSY 240

Query: 241 TNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE 300
           TNVFDANYDTLIWSLKK+G  ++KI++GEVGWPTD N FANV LAKRFYDGLF+KLASG+
Sbjct: 241 TNVFDANYDTLIWSLKKLGVSDMKIIVGEVGWPTDGNKFANVNLAKRFYDGLFKKLASGD 300

Query: 301 GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA 360
           GTPMRPKE FEVYLFGLLDE+ KSVLPGFFERHWGIFQFDGKPKFPMD SGKGNDKMLVA
Sbjct: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDSSGKGNDKMLVA 360

Query: 361 AKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFN 420
           AKGVQY+E+KWCVL++ +K  +  + +VDYAC  SDCT+L  G SC  LDRRG ISYAFN
Sbjct: 361 AKGVQYLEKKWCVLKKNVKVFDNISPQVDYACSLSDCTSLGYGSSCNNLDRRGNISYAFN 420

Query: 421 IYFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLKAAAAAMVGLVLSLFV 480
           +Y+QMQDQ+VEACVF+G+AEIV  NA+VG+CLFPIQIVS GERLKAAAAAMVGLVLSLF 
Sbjct: 421 MYYQMQDQSVEACVFNGSAEIVKNNASVGSCLFPIQIVSAGERLKAAAAAMVGLVLSLFA 480

Query: 481 LGL 484
           L L
Sbjct: 481 LRL 483

BLAST of Carg05274 vs. TAIR10
Match: AT5G64790.1 (O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 549.7 bits (1415), Expect = 1.8e-156
Identity = 259/482 (53.73%), Postives = 354/482 (73.44%), Query Frame = 0

Query: 2   GGATIVPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKLF 61
           G A ++      ++TV  +A+ V  GG GVNWG +ASHPL P+IVV +LKDN I KVKLF
Sbjct: 3   GRAAMLVNVGTVIMTVLTLASLV--GGFGVNWGNIASHPLNPNIVVQMLKDNKINKVKLF 62

Query: 62  DSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVSV 121
           D+D+WT+++LAG+ +E ++GIPN+ L+  A+ Y+ AK+WVK+NVT ++  GGVDI+YV+V
Sbjct: 63  DADSWTMNALAGTGMEVMVGIPNNLLESLADDYDNAKDWVKENVTQYIRKGGVDIKYVAV 122

Query: 122 GNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQF 181
           GNE FL+AYNGSF+  TFPA++N+ KAL EAGH+  +K T   NA+VY++ ++ PS+G F
Sbjct: 123 GNEPFLSAYNGSFLKTTFPALKNIHKALKEAGHTNIMKATIPQNAEVYQSANDKPSEGDF 182

Query: 182 RPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSYT 241
           R D+   M +IV F   +D PF VNIYPFLSLY N +FP+++AF  G GQ+  D GK+Y 
Sbjct: 183 RKDVKQTMLDIVNFFHENDLPFTVNIYPFLSLYLNEHFPVEFAFLDGDGQTMTDKGKNYD 242

Query: 242 NVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGEG 301
           NVFDANYDTL+++LKK G  ++KI++GEVGWPTD + +A+  LA++FY GL ++LA   G
Sbjct: 243 NVFDANYDTLVYALKKAGIHDMKIIVGEVGWPTDGHKYASPKLAEKFYAGLMKRLAKDGG 302

Query: 302 TPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVAA 361
           TP RP E  EVYLFG LDE+ KS+LPG FERHWGIF++DG PKF +D +G+G   + VAA
Sbjct: 303 TPTRP-ERLEVYLFGFLDEDMKSILPGPFERHWGIFRYDGTPKFMLDFTGQGRQMVPVAA 362

Query: 362 KGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFNI 421
           KGVQY+E++WCV+ +   NL+    ++DYAC + DCTA+ AG +C +L +   ISYAFN+
Sbjct: 363 KGVQYLEKQWCVVNKDTVNLDEVGPDLDYACYHGDCTAMEAGSTCSKLTKVQNISYAFNM 422

Query: 422 YFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLKAAAAAMVGLVLSLFVL 481
           YFQ+QDQ+V AC F G A I   NA+VG+CLFP+QIVSG +  +        +V  L +L
Sbjct: 423 YFQIQDQDVRACDFKGAAMITKVNASVGSCLFPVQIVSGSDDFRINFVFGRFVVFGLVLL 481

Query: 482 GL 484
           GL
Sbjct: 483 GL 481

BLAST of Carg05274 vs. TAIR10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 515.0 bits (1325), Expect = 5.0e-146
Identity = 249/464 (53.66%), Postives = 331/464 (71.34%), Query Frame = 0

Query: 23  GVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKLFDSDTWTVSSLAGSKIETIIGI 82
           G+   G+GVNWGT+A+H L P  VV +LKDN I KVKLFD+D  T+ +LAGS +E ++ I
Sbjct: 19  GILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVMVAI 78

Query: 83  PNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVSVGNEAFLTAYNGSFVNVTFPAM 142
           PND+LK    SY+ AK+WV+ NVT + +DGGV+I +V+VGNE FL +YNGSF+N+TFPA+
Sbjct: 79  PNDQLKVMT-SYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPAL 138

Query: 143 QNVQKALDEAGHSKKIKVTTALNADVYE--AKSNLPSDGQFRPDIYSIMKEIVRFLDRHD 202
            N+Q AL+EAG    +K T  LNADVY+  A + +PS G+FRPDI   M +IV FL +++
Sbjct: 139 ANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNN 198

Query: 203 APFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSYTNVFDANYDTLIWSLKKVGF 262
           AP  +NIYPFLSLY N +FP++YAFF G+ +  NDNG  YTNVFDAN+DTL+ SLK VG 
Sbjct: 199 APITINIYPFLSLYGNDDFPLNYAFFDGA-EPINDNGIDYTNVFDANFDTLVSSLKAVGH 258

Query: 263 PNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGEGTPMRPKESFEVYLFGLLDE 322
            ++ I++GEVGWPT+ +  AN   A RFY+GL  +L + +GTP+RP    EVYLFGLLDE
Sbjct: 259 GDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRP-TYIEVYLFGLLDE 318

Query: 323 NAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVAAKGVQYMERKWCVLRQTLKN 382
           +AKS+ PG FERHWGIF+FDG+PKFP+DLSG+G  K L+ A+ V Y+  KWC      K+
Sbjct: 319 DAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKD 378

Query: 383 LEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFNIYFQMQDQNVEACVFDGTAE 442
           L   A  +DYAC +SDCTAL  G SC  LD  G  SYAFN++FQ+++Q+  AC F G A 
Sbjct: 379 LTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLAT 438

Query: 443 IVTKNATVGNCLFPIQIV-SGGERLKAAAAAMVGLVLSLFVLGL 484
           I T+N + G C FPIQIV S       ++ ++V L++   + G+
Sbjct: 439 ITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWFLLSGM 479

BLAST of Carg05274 vs. TAIR10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 515.0 bits (1325), Expect = 5.0e-146
Identity = 244/434 (56.22%), Postives = 322/434 (74.19%), Query Frame = 0

Query: 28  GIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKLFDSDTWTVSSLAGSKIETIIGIPNDRL 87
           G+GVNWGT+A+H L P  VV +LKDN I KVKLFD+D  T+S+L+GS +E ++ IPND+L
Sbjct: 20  GLGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTMSALSGSGLEVMVAIPNDQL 79

Query: 88  KKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVSVGNEAFLTAYNGSFVNVTFPAMQNVQK 147
           K    SY+ AK+WV  NVT + ++GGV+I +V+VGNE FL +YNGSF+N+TFPA+QN+Q 
Sbjct: 80  KVMG-SYDRAKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKSYNGSFINLTFPALQNIQN 139

Query: 148 ALDEAGHSKKIKVTTALNADVYEAKSN--LPSDGQFRPDIYSIMKEIVRFLDRHDAPFMV 207
           AL+EAG    +K T  LNADVY++ S+  +PS G+FRPDI   M +IV FL  + AP  +
Sbjct: 140 ALNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIGQMTQIVDFLGNNSAPITI 199

Query: 208 NIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSYTNVFDANYDTLIWSLKKVGFPNLKI 267
           NIYPFLSLY N +FP++YAFF G+ +  +DNG +YTNVFDAN+DTL+ +LK VG  ++ I
Sbjct: 200 NIYPFLSLYGNDDFPLNYAFFDGA-KPVDDNGIAYTNVFDANFDTLVSALKAVGHGDMPI 259

Query: 268 VIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGEGTPMRPKESFEVYLFGLLDENAKSV 327
           ++GEVGWPT+ +  AN   A RFY+GL  +L    GTP+RP    EVYLFGLLDE+AKS+
Sbjct: 260 IVGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLRP-TYIEVYLFGLLDEDAKSI 319

Query: 328 LPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVAAKGVQYMERKWCVLRQTLKNLEPTA 387
            PG FERHWGIF+FDG+PKFP+DLSG+G +K+L+ A+ V Y  +KWC+     K+L   A
Sbjct: 320 APGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCMFNTEAKDLTKLA 379

Query: 388 KEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFNIYFQMQDQNVEACVFDGTAEIVTKN 447
             +DYAC +SDCTAL  G SC  LD  G  SYAFN+YFQ+++Q+ +AC+F G A I TKN
Sbjct: 380 ANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVKNQDEDACIFQGLATITTKN 439

Query: 448 ATVGNCLFPIQIVS 460
            + G C FPIQIV+
Sbjct: 440 ISQGQCNFPIQIVA 450

BLAST of Carg05274 vs. TAIR10
Match: AT5G18220.1 (O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 493.4 bits (1269), Expect = 1.6e-139
Identity = 247/449 (55.01%), Postives = 317/449 (70.60%), Query Frame = 0

Query: 15  ITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKLFDSDTWTVSSLAGS 74
           IT+ ++ +G     +GVNWGT+++H L P  VV +LKDN +KKVKLFD+DT T+ +LAGS
Sbjct: 17  ITIVSLLSGDV-SALGVNWGTMSTHQLPPKTVVQMLKDNNVKKVKLFDADTNTMVALAGS 76

Query: 75  KIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVSVGNEAFLTAYNGSF 134
            IE ++ IPND+LK    SYN AK+WV+ N+T   ++  V I+YV+VGNE FLTAYNGSF
Sbjct: 77  GIEVMVAIPNDQLKAMG-SYNRAKDWVRRNIT--RFNDDVKIKYVAVGNEPFLTAYNGSF 136

Query: 135 VNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEA--KSNLPSDGQFRPDIYSIMKEI 194
           +N+T+PA+ N+QKAL+EAG    IK T  LNADVY +  ++ +PS G FR DI+  MK I
Sbjct: 137 INLTYPALFNIQKALNEAGVGDFIKATVPLNADVYNSPLENPVPSAGSFRQDIFEEMKLI 196

Query: 195 VRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSYTNVFDANYDTLI 254
           V FL  + APF VNIYPFLSLY + +FP DYAFF G   + +DNG  YTNVFDAN+DTL+
Sbjct: 197 VNFLAHNKAPFTVNIYPFLSLYLSSDFPFDYAFFNGQ-NTVSDNGVIYTNVFDANFDTLL 256

Query: 255 WSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGEGTPMRPKESFEV 314
            SLK +G  ++ +++GEVGWPTD +  AN+  A+RFY GL  KLA+  GTPMRP    EV
Sbjct: 257 ASLKALGHGDMTVIVGEVGWPTDGDKNANIPNAERFYTGLLPKLAANRGTPMRP-GYIEV 316

Query: 315 YLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVAAKGVQYMERKWC 374
           YLFG +DE+AKS+ PG FERHWGIF++DG+PKFP DLSG G  K+L  A+ VQY+  +WC
Sbjct: 317 YLFGFIDEDAKSIAPGNFERHWGIFKYDGQPKFPADLSGAGQKKILTGAQNVQYLRNQWC 376

Query: 375 VLRQTL----KNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFNIYFQMQDQ 434
           +          N       V+YAC +SDCTAL  G SCG LD  G  SYAFN+YFQ+Q+Q
Sbjct: 377 MFNPNALTFSNNTNQLGDNVNYACTFSDCTALGYGSSCGNLDEVGNASYAFNMYFQVQNQ 436

Query: 435 NVEACVFDGTAEIVTKNATVGNCLFPIQI 458
             EAC F+G A I T+N +   C FPIQI
Sbjct: 437 KAEACDFEGLAIITTRNISREQCNFPIQI 459

BLAST of Carg05274 vs. TAIR10
Match: AT3G04010.1 (O-Glycosyl hydrolases family 17 protein)

HSP 1 Score: 485.3 bits (1248), Expect = 4.3e-137
Identity = 241/447 (53.91%), Postives = 314/447 (70.25%), Query Frame = 0

Query: 14  VITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKLFDSDTWTVSSLAG 73
           +  V  ++ GV+  G+GVNWGT+ASH L P  VV +LKDN I+KVKLFD+DT T+ +LAG
Sbjct: 20  ISAVCFLSGGVS--GLGVNWGTMASHQLPPKTVVEMLKDNNIQKVKLFDADTNTMGALAG 79

Query: 74  SKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVSVGNEAFLTAYNGS 133
           S +E ++ IPND L     +Y  AK+WV+ NV+   ++ GV I+YV+VGNE FLTAYNGS
Sbjct: 80  SGVEVMVAIPNDLLLAMG-NYQRAKDWVQRNVSRFNFNNGVKIKYVAVGNEPFLTAYNGS 139

Query: 134 FVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEA--KSNLPSDGQFRPDIYSIMKE 193
           F+N+T+PA+ N+Q AL+EAG     K T  LNADVY +   + +PS G+FR DI   M +
Sbjct: 140 FINLTYPALFNIQTALNEAGVGDFTKATVPLNADVYNSPPDNQVPSAGRFRSDIIQEMTQ 199

Query: 194 IVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSYTNVFDANYDTL 253
           IV FL ++ APF VNIYPFLSLY + +FP +YAFF G   + NDNG  YTNVFDA++DTL
Sbjct: 200 IVNFLAQNKAPFTVNIYPFLSLYLSSDFPFEYAFFDGQ-NTVNDNGVIYTNVFDASFDTL 259

Query: 254 IWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGEGTPMRPKESFE 313
           + SL  +   N+++++GEVGWPTD +  ANV  A+RFY GL  +LA+  GTPMR K   E
Sbjct: 260 LASLNALNHGNMEVIVGEVGWPTDGDKNANVPNAERFYSGLLPRLANNVGTPMR-KGYIE 319

Query: 314 VYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVAAKGVQYMERKW 373
           VYLFG +DE+AKSV PG FERHWGIF+FDG+PKFP+D  G+G  K L  A+ VQY   +W
Sbjct: 320 VYLFGFIDEDAKSVAPGNFERHWGIFKFDGQPKFPVDFRGQGQKKFLTGAQNVQYFLNQW 379

Query: 374 CVLRQTLK-NLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFNIYFQMQDQNV 433
           C+     + N+      ++YAC +SDCTAL  G SCG LD  G  SYAFN+YFQ+Q+Q  
Sbjct: 380 CMFNPNGRGNMSRLGDNINYACSHSDCTALGYGSSCGNLDANGNASYAFNMYFQVQNQEA 439

Query: 434 EACVFDGTAEIVTKNATVGNCLFPIQI 458
           +AC F+G A I T+N + G C FPIQI
Sbjct: 440 QACDFEGLATITTQNISQGQCNFPIQI 461

BLAST of Carg05274 vs. Swiss-Prot
Match: sp|Q6NKW9|E138_ARATH (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 515.0 bits (1325), Expect = 9.0e-145
Identity = 249/464 (53.66%), Postives = 331/464 (71.34%), Query Frame = 0

Query: 23  GVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKLFDSDTWTVSSLAGSKIETIIGI 82
           G+   G+GVNWGT+A+H L P  VV +LKDN I KVKLFD+D  T+ +LAGS +E ++ I
Sbjct: 19  GILVNGLGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVMVAI 78

Query: 83  PNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVSVGNEAFLTAYNGSFVNVTFPAM 142
           PND+LK    SY+ AK+WV+ NVT + +DGGV+I +V+VGNE FL +YNGSF+N+TFPA+
Sbjct: 79  PNDQLKVMT-SYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFPAL 138

Query: 143 QNVQKALDEAGHSKKIKVTTALNADVYE--AKSNLPSDGQFRPDIYSIMKEIVRFLDRHD 202
            N+Q AL+EAG    +K T  LNADVY+  A + +PS G+FRPDI   M +IV FL +++
Sbjct: 139 ANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGKNN 198

Query: 203 APFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSYTNVFDANYDTLIWSLKKVGF 262
           AP  +NIYPFLSLY N +FP++YAFF G+ +  NDNG  YTNVFDAN+DTL+ SLK VG 
Sbjct: 199 APITINIYPFLSLYGNDDFPLNYAFFDGA-EPINDNGIDYTNVFDANFDTLVSSLKAVGH 258

Query: 263 PNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGEGTPMRPKESFEVYLFGLLDE 322
            ++ I++GEVGWPT+ +  AN   A RFY+GL  +L + +GTP+RP    EVYLFGLLDE
Sbjct: 259 GDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRP-TYIEVYLFGLLDE 318

Query: 323 NAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVAAKGVQYMERKWCVLRQTLKN 382
           +AKS+ PG FERHWGIF+FDG+PKFP+DLSG+G  K L+ A+ V Y+  KWC      K+
Sbjct: 319 DAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEAKD 378

Query: 383 LEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFNIYFQMQDQNVEACVFDGTAE 442
           L   A  +DYAC +SDCTAL  G SC  LD  G  SYAFN++FQ+++Q+  AC F G A 
Sbjct: 379 LTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGLAT 438

Query: 443 IVTKNATVGNCLFPIQIV-SGGERLKAAAAAMVGLVLSLFVLGL 484
           I T+N + G C FPIQIV S       ++ ++V L++   + G+
Sbjct: 439 ITTQNISQGQCNFPIQIVASSASSFSCSSYSLVVLIVWFLLSGM 479

BLAST of Carg05274 vs. Swiss-Prot
Match: sp|Q9M088|E135_ARATH (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 6.1e-133
Identity = 234/469 (49.89%), Postives = 322/469 (68.66%), Query Frame = 0

Query: 14  VITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKLFDSDTWTVSSLAG 73
           VIT+   +  +   GIGVNWG+ A HPL P+ VV LL++NGI+KVKLF++D+  + +L+ 
Sbjct: 12  VITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKALSR 71

Query: 74  SKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVSVGNEAFLTAYNGS 133
           + I+ ++GIPND L   A S   A+ WV  NV+ H+   GVDIRYV+VGNE FL A+NG+
Sbjct: 72  TGIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAFNGT 131

Query: 134 FVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQFRPDIYSIMKEIV 193
           F  +T PA+QN+Q A+ +AG + ++KVT  LNADVY++ SNLPSDG FRP+I  +M  IV
Sbjct: 132 FEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQSASNLPSDGDFRPEIRDLMLNIV 191

Query: 194 RFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSYTNVFDANYDTLIW 253
           +FL  + APF +NIYPF+SLY +PNFP+++AFF G+G   NDNG+ Y NV DANYDTL+W
Sbjct: 192 KFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDANYDTLVW 251

Query: 254 SLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGEGTPMRPKESFEVY 313
           SL+K GF NL I++GEVGWPTD +  AN+  A+R+  G   +  + +GTPMRP  + + Y
Sbjct: 252 SLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRP-GAMDAY 311

Query: 314 LFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVAAKGVQYMERKWCV 373
           LFGL+DE+AKS+ PG FERHWGIF  DG+PK+ + L G GN   L+ AK V Y+ +KWC+
Sbjct: 312 LFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSL-GSGNG--LIPAKDVHYLAKKWCI 371

Query: 374 LRQTLKNLEP-TAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFNIYFQMQDQNVEA 433
           L       +P     V YAC ++DCT+L  G SCG L+    +SYAFN Y+Q+ +Q   A
Sbjct: 372 LAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQLDSA 431

Query: 434 CVFDGTAEIVTKNATVGNCLFPIQIVSGGERLKAAAAAMVGLVLSLFVL 482
           C F G + + T++ +VG+C F I I S      + A+AM+ +  S  VL
Sbjct: 432 CKFPGLSIVSTRDPSVGSCKFKIMIKSED---ASEASAMMPITRSTAVL 473

BLAST of Carg05274 vs. Swiss-Prot
Match: sp|Q93Z08|E136_ARATH (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 469.5 bits (1207), Expect = 4.4e-131
Identity = 227/449 (50.56%), Postives = 306/449 (68.15%), Query Frame = 0

Query: 11  WAAVITVAAMAAGVAE-GGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKLFDSDTWTVS 70
           W +V+ + A+A        IG NWGT ASHPL P IVV +L++NGI+KVKLFD++  T+ 
Sbjct: 3   WGSVLLLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDTLR 62

Query: 71  SLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVSVGNEAFLTA 130
           +L  S IE ++GIPN+ L   A S   A+ WV  NV+ H+    V+IRYV+VGNE FL+ 
Sbjct: 63  ALGKSGIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFLST 122

Query: 131 YNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQFRPDIYSIM 190
           YNGS+++ TFPA++N+Q A+ +AG   ++KVT  LNADVY++ +  PS G FR +I  +M
Sbjct: 123 YNGSYLSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDSSTTFPSGGDFRANIRDLM 182

Query: 191 KEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSYTNVFDANYD 250
             IV+FL  +  PF VNIYP++SLY NP+FP+DYAFF G+ Q  ND G  Y N+FDANYD
Sbjct: 183 ITIVKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYNMFDANYD 242

Query: 251 TLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGEGTPMRPKES 310
           TL+ +L+K GF N+ I+IGE+GWPTD +  AN+  AK+F  G    ++ G+GTP RP   
Sbjct: 243 TLVHALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRRP-GP 302

Query: 311 FEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVAAKGVQYMER 370
            + YLF L+DE+AKSV PG+FERHWGIF FDG PK+ ++L G  N   L+ AKGV+Y+ER
Sbjct: 303 IDAYLFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNL-GTTNTGALIQAKGVRYLER 362

Query: 371 KWCVLRQTLKNLEP-TAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFNIYFQMQDQ 430
           KWCV++  ++  +P  A  V YAC   DCT+L  G SC  LD +  ISYAFN Y+Q+QDQ
Sbjct: 363 KWCVMKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQDQ 422

Query: 431 NVEACVFDGTAEIVTKNATVGNCLFPIQI 458
              AC F   +E+   + + G C FPI I
Sbjct: 423 LDTACKFPNISEVTKTDPSTGTCRFPIMI 449

BLAST of Carg05274 vs. Swiss-Prot
Match: sp|Q9FGH4|E139_ARATH (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 3.0e-92
Identity = 179/444 (40.32%), Postives = 251/444 (56.53%), Query Frame = 0

Query: 15  ITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKLFDSDTWTVSSLAGS 74
           +T      G     +G+NWGT ASHPL PS VV LLK NGI KVKLFD+D   + +L+GS
Sbjct: 12  VTAGLSLTGTTVRAVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKVLRALSGS 71

Query: 75  KIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGG-VDIRYVSVGNEAFLTAYNGS 134
            I   IGI N  LK    S   A++WV DNVT +   G  V I YV+VG E FL +Y   
Sbjct: 72  NIGVTIGIQNSMLKSLNASVKVAESWVHDNVTRYFNGGNRVRIEYVAVGEEPFLQSYGNQ 131

Query: 135 FVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQFRPDIYSIMKEIV 194
           +      A  N+Q AL +A  + ++KV    + D + ++S  PS G FR D+   M E++
Sbjct: 132 YKPFVIGAAMNIQNALVKANLANEVKVVVPSSFDSFLSESGRPSSGHFRADLNKTMIELL 191

Query: 195 RFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSYTNVFDANYDTLIW 254
            FL +H +PF V I PFLS +QN N  +D++ F  + +++ D  K+Y N FD +YDTL+ 
Sbjct: 192 SFLTKHHSPFFVTISPFLSFHQNKNISLDFSLFKETAKAHKDGRKTYRNSFDLSYDTLVS 251

Query: 255 SLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGEGTPMRPKESFEVY 314
           +L  +GF  + IV+ ++GWPTD    A    A+ F+ GL   L   + T   P+   E Y
Sbjct: 252 ALFTIGFSEVDIVVSKIGWPTDGAENATSLTAEAFFKGLIVHLE--KKTASLPRPPVETY 311

Query: 315 LFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVAAKGVQYMERKWCV 374
           +  LLDE+ +++  G FERHWG+F FDG+ K+        N K  V A+ VQY+  KWCV
Sbjct: 312 IESLLDEDQRNLSAGNFERHWGVFTFDGQAKYNFSF----NHKNQVNAQNVQYLPPKWCV 371

Query: 375 LRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFNIYFQMQDQNVEAC 434
           +    K+L   +     AC  +DCT++  GGSC  +   G +SYAFN  +Q  D + E+C
Sbjct: 372 VNNN-KDLSNASARALEACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQNDHSAESC 431

Query: 435 VFDGTAEIVTKNATVGNCLFPIQI 458
            F G   I T + +  NC F IQ+
Sbjct: 432 NFGGLGLITTVDPSEDNCRFSIQL 448

BLAST of Carg05274 vs. Swiss-Prot
Match: sp|Q9M069|E137_ARATH (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 229.9 bits (585), Expect = 5.8e-59
Identity = 154/439 (35.08%), Postives = 232/439 (52.85%), Query Frame = 0

Query: 25  AEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKLFDSDTWTVSSLAGSKIETIIGIPN 84
           AE  IGVN+G +A +   PS  V LL+   I+KV+L+ +D   + +LAG+ +  +IG  N
Sbjct: 22  AEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAAN 81

Query: 85  DRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVSVGNEAFLTAYNGSFVNVTFPAMQN 144
             +   A   N A  W+  NV +  Y     I  ++VGNE  L + + + VN   PAMQN
Sbjct: 82  GDVPSLASDPNAATQWINSNV-LPFYPAS-KIMLITVGNE-ILMSNDPNLVNQLLPAMQN 141

Query: 145 VQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQFRPDIYSIMKEIVRFLDRHDAPFM 204
           VQKAL+      KIKV+T +N+      S+ PS G F     + +K I++FL    +PF 
Sbjct: 142 VQKALEAVSLGGKIKVST-VNSMTVLGSSDPPSSGSFAAGYQTGLKGILQFLSDTGSPFA 201

Query: 205 VNIYPFLSLYQNPNFPIDYAFF---GGSGQSNNDNGKSYTNVFDANYDTLIWSLKKVGFP 264
           +N YPF + YQ+   P   AF      +G+ ++  G  YTN+FDA  D +  +LK +GF 
Sbjct: 202 INPYPFFA-YQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFE 261

Query: 265 NLKIVIGEVGWPT--DAN-VFANVTLAKRFYDGLFEKLASGEGTPMRPKESFEVYLFGLL 324
            ++IV+ E GW +  DAN V A+V  AK +   L   L S  GTP+ P +  + Y+F L 
Sbjct: 262 KVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALY 321

Query: 325 DENAKSVLPG-FFERHWGIFQFDGKPKFPMDLSGKGNDKMLVAAKGVQYMERKWCVLRQT 384
           DEN K   PG   ER +G+F+ D    + + L+   +     + K        WCV ++ 
Sbjct: 322 DENLK---PGPSSERAFGLFKTDLSMVYDVGLAKSSSSSQTPSGK---VTSSGWCVPKKG 381

Query: 385 LKNLEPTAKEVDYACGYS-DCTALTAGGSCGQLDR-RGRISYAFNIYFQMQDQNVEACVF 444
             N E  A  +D+ACG+  DC A+  GG+C + +      +YA N+YFQ   +    C F
Sbjct: 382 ATNEELQA-SLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTDCDF 441

Query: 445 DGTAEIVTKNATVGNCLFP 455
             TA + ++N +  NC++P
Sbjct: 442 SKTATVTSQNPSYNNCVYP 448

BLAST of Carg05274 vs. TrEMBL
Match: tr|A0A1S3B101|A0A1S3B101_CUCME (glucan endo-1,3-beta-glucosidase 8 OS=Cucumis melo OX=3656 GN=LOC103484682 PE=3 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 2.7e-226
Identity = 375/484 (77.48%), Postives = 432/484 (89.26%), Query Frame = 0

Query: 1   MGGATIVPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL 60
           M G +IVP  WAAV+TVAA+ + V   GIG NWG ++SHPL+P+IVV LLKDNGIKKVKL
Sbjct: 1   MTGVSIVPAVWAAVMTVAAVFSVVEGAGIGANWGIMSSHPLRPNIVVKLLKDNGIKKVKL 60

Query: 61  FDSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVS 120
           FDSD+WTVS+L+GSKIET+IGIPND+LKKFAESY++AK+WVK+NVT HM++GGVD+RYVS
Sbjct: 61  FDSDSWTVSALSGSKIETVIGIPNDQLKKFAESYDDAKDWVKENVTTHMFEGGVDLRYVS 120

Query: 121 VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQ 180
           VGNEAFL+AYNG++V VT+PAMQN+QKALD AGH KK+KVTTALNADVYE+ +NLPSDGQ
Sbjct: 121 VGNEAFLSAYNGTYVKVTYPAMQNIQKALDAAGHGKKVKVTTALNADVYESSTNLPSDGQ 180

Query: 181 FRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSY 240
           FRPDIY  MK+IV FLDR+ APFMVNIYPFLSLYQNPNFP+DYAFFGG  ++ ND GKSY
Sbjct: 181 FRPDIYKTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFGGGAKATNDKGKSY 240

Query: 241 TNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE 300
           TNVFDANYDTLIWSLKK+G  ++KI++GEVGWPTD N FANV LAKRFYDGLF+KLASG 
Sbjct: 241 TNVFDANYDTLIWSLKKIGVTDMKIIVGEVGWPTDGNKFANVELAKRFYDGLFKKLASGN 300

Query: 301 GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA 360
           GTPMRPKE FEVYLFGLLDE+ KSVLPGFFERHWGIFQFDGKPKFPMD+SGKGNDKMLVA
Sbjct: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDMSGKGNDKMLVA 360

Query: 361 AKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFN 420
           AKGVQY+E+KWCVL++ +KN +  +KEVDYACG SDCT+L  G SC  LD+RG ISYAFN
Sbjct: 361 AKGVQYLEKKWCVLKENVKNFDKISKEVDYACGLSDCTSLGYGSSCNNLDKRGNISYAFN 420

Query: 421 IYFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLK-AAAAAMVGLVLSLF 480
           +Y+QMQDQ+VEAC F G+A IV KNA+VG+CLFPIQIVS GERLK AAAAA+VGLVL LF
Sbjct: 421 MYYQMQDQSVEACDFSGSAHIVKKNASVGSCLFPIQIVSTGERLKAAAAAAVVGLVLGLF 480

Query: 481 VLGL 484
            LGL
Sbjct: 481 ALGL 484

BLAST of Carg05274 vs. TrEMBL
Match: tr|A0A0A0KJ60|A0A0A0KJ60_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G486900 PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 7.7e-226
Identity = 377/483 (78.05%), Postives = 430/483 (89.03%), Query Frame = 0

Query: 1   MGGATIVPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKL 60
           M G ++VP  WAAV+T+ A+ + V   GIGVNWG L+SHPL+P+IVV LLKDNGIKKVKL
Sbjct: 1   MAGVSMVPAVWAAVMTLGAVFSVVEGAGIGVNWGILSSHPLRPNIVVKLLKDNGIKKVKL 60

Query: 61  FDSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVS 120
           FDSD WTVSSL+GSKIETIIGIPND+LKKFA+SY++AK+WVK+NVT HM++GGVD+RYVS
Sbjct: 61  FDSDAWTVSSLSGSKIETIIGIPNDQLKKFAKSYDDAKDWVKENVTSHMFEGGVDLRYVS 120

Query: 121 VGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQ 180
           VGNEAFLTAYNGSFV VTFPAMQN+QKA+D AGH KKIKVTTALNADVYE+ +NLPSDG+
Sbjct: 121 VGNEAFLTAYNGSFVKVTFPAMQNIQKAIDAAGHGKKIKVTTALNADVYESSTNLPSDGE 180

Query: 181 FRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSY 240
           FRPDIYS MK+IV FLDR+ APFMVNIYPFLSLYQNPNFP+DYAFF G G++ ND  KSY
Sbjct: 181 FRPDIYSTMKDIVHFLDRNKAPFMVNIYPFLSLYQNPNFPLDYAFFDGGGKATNDKDKSY 240

Query: 241 TNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGE 300
           TNVFDANYDTLIWSLKK+G  ++KI++GEVGWPTD N FANV LAKRFYDGLF+KLASG+
Sbjct: 241 TNVFDANYDTLIWSLKKLGVSDMKIIVGEVGWPTDGNKFANVNLAKRFYDGLFKKLASGD 300

Query: 301 GTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVA 360
           GTPMRPKE FEVYLFGLLDE+ KSVLPGFFERHWGIFQFDGKPKFPMD SGKGNDKMLVA
Sbjct: 301 GTPMRPKEKFEVYLFGLLDEDMKSVLPGFFERHWGIFQFDGKPKFPMDSSGKGNDKMLVA 360

Query: 361 AKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFN 420
           AKGVQY+E+KWCVL++ +K  +  + +VDYAC  SDCT+L  G SC  LDRRG ISYAFN
Sbjct: 361 AKGVQYLEKKWCVLKKNVKVFDNISPQVDYACSLSDCTSLGYGSSCNNLDRRGNISYAFN 420

Query: 421 IYFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLKAAAAAMVGLVLSLFV 480
           +Y+QMQDQ+VEACVF+G+AEIV  NA+VG+CLFPIQIVS GERLKAAAAAMVGLVLSLF 
Sbjct: 421 MYYQMQDQSVEACVFNGSAEIVKNNASVGSCLFPIQIVSAGERLKAAAAAMVGLVLSLFA 480

Query: 481 LGL 484
           L L
Sbjct: 481 LRL 483

BLAST of Carg05274 vs. TrEMBL
Match: tr|A0A0A0LLR5|A0A0A0LLR5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G277010 PE=3 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 3.2e-195
Identity = 326/487 (66.94%), Postives = 397/487 (81.52%), Query Frame = 0

Query: 1   MGGATI-VPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVK 60
           M GAT  +   W  +I V      V +  +G NWGT+ASHPL P+IVVNLL+DNGIKKVK
Sbjct: 1   MTGATFAIAVTWTVLIVVTTTITSVVQAHLGANWGTMASHPLNPNIVVNLLEDNGIKKVK 60

Query: 61  LFDSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYV 120
           LFDSD+WTVS+LAGSKIETI+GIPND+L+  A  YN AK+WVK+NVT H+YDGGV+IRY+
Sbjct: 61  LFDSDSWTVSALAGSKIETIVGIPNDQLESLASDYNHAKDWVKENVTAHIYDGGVNIRYI 120

Query: 121 SVGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDG 180
           +VGNE FLTAYNG+++ +TFPAMQN+QKALDEAG+SKKIKVT  LNADVYE+ +N PSDG
Sbjct: 121 AVGNEPFLTAYNGTYIKLTFPAMQNIQKALDEAGYSKKIKVTCPLNADVYESATNQPSDG 180

Query: 181 QFRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKS 240
           QFR DI   MK+IVRFL R+DA FMVNIYPFLSLY N +FP+D+AFF  +G+S ND GK 
Sbjct: 181 QFRSDILDEMKDIVRFLSRNDAAFMVNIYPFLSLYLNSDFPVDFAFFDENGKSINDKGKK 240

Query: 241 YTNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASG 300
           YTNVFDAN+DTL+WSLKK+G  +LKI++GEVGWPTD N FA V LAKRFYDGL +KLAS 
Sbjct: 241 YTNVFDANFDTLVWSLKKIGLGDLKIIVGEVGWPTDGNKFATVELAKRFYDGLLKKLASK 300

Query: 301 EGTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLV 360
           +GTPMRP E  EVYLFGLLDE+ KS+ PGFFERHWG+F++DGKPKF +DL+GKGNDK LV
Sbjct: 301 KGTPMRPNEKLEVYLFGLLDEDLKSIQPGFFERHWGLFRYDGKPKFSLDLTGKGNDKRLV 360

Query: 361 AAKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAF 420
           AAKGVQY+E KWCV++ ++K+L   + ++DYAC  SDCT+L  G SC  L+ RG ISYA+
Sbjct: 361 AAKGVQYLEHKWCVVKNSVKDLGTISSQIDYACSMSDCTSLGYGSSCNNLNSRGNISYAY 420

Query: 421 NIYFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLKA------AAAAMVG 480
           N+YFQMQDQ+VEACVF  +AEIVT+NA+VG+CLFPIQI S G+RL         A  ++G
Sbjct: 421 NMYFQMQDQSVEACVFGESAEIVTRNASVGSCLFPIQIDSIGQRLTPMNIVVFTATVLIG 480

BLAST of Carg05274 vs. TrEMBL
Match: tr|A0A1S3C7C2|A0A1S3C7C2_CUCME (glucan endo-1,3-beta-glucosidase 8-like OS=Cucumis melo OX=3656 GN=LOC103497517 PE=3 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 2.1e-194
Identity = 323/487 (66.32%), Postives = 396/487 (81.31%), Query Frame = 0

Query: 1   MGGATI-VPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVK 60
           M  AT  +   W  +I V      V +  +GVNWG++ASHPL P+IVVNLLKDNGIKKVK
Sbjct: 1   MASATFAIAVTWTVLIVVTTTVTSVVQANLGVNWGSMASHPLNPNIVVNLLKDNGIKKVK 60

Query: 61  LFDSDTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYV 120
           LFDSD+WTV++L+GSKIETI+GIPND+L+  A +YN AK+WVK+N+T H+YDGGV+IRYV
Sbjct: 61  LFDSDSWTVNALSGSKIETIVGIPNDQLESLATNYNHAKDWVKENITAHIYDGGVNIRYV 120

Query: 121 SVGNEAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDG 180
           +VGNE FLTAYNG++V +TFPAMQN+QKAL+EAG++KKIKVT  LNADVYE+ SN PSDG
Sbjct: 121 AVGNEPFLTAYNGTYVKLTFPAMQNIQKALNEAGYNKKIKVTCPLNADVYESASNHPSDG 180

Query: 181 QFRPDIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKS 240
           QFR DI   MKEIV+FLDR++A FMVNIYPFLSLY NP+FP+D+AF   +G+S ND GK+
Sbjct: 181 QFRSDILDEMKEIVQFLDRNNAAFMVNIYPFLSLYLNPDFPVDFAFMDENGRSINDKGKT 240

Query: 241 YTNVFDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASG 300
           YTNV DANYDTL+WSLKK+G  ++KI++GEVGWPTD N FA V LAKRFYDGL +KLAS 
Sbjct: 241 YTNVLDANYDTLVWSLKKIGHGDMKIIVGEVGWPTDGNKFATVGLAKRFYDGLLKKLASK 300

Query: 301 EGTPMRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLV 360
           +GTPMRP E  EVYLFGL DE+ KS+ PGFFERHWG+F++DGKPKFP+D +GK NDKMLV
Sbjct: 301 KGTPMRPNEKLEVYLFGLFDEDTKSIQPGFFERHWGLFRYDGKPKFPIDFTGKDNDKMLV 360

Query: 361 AAKGVQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAF 420
           AAKGVQY+E KWCV++ ++K+L   + +VDYAC  SDCT L  G SC  L+ +G ISYA+
Sbjct: 361 AAKGVQYLEHKWCVVKNSVKDLRTISSQVDYACSMSDCTTLGYGSSCNNLNAKGNISYAY 420

Query: 421 NIYFQMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLKA------AAAAMVG 480
           N+YFQMQDQ+VEACVF  +AEIVTKNA+VGNCLFPIQIVS G+RL            +V 
Sbjct: 421 NMYFQMQDQSVEACVFGDSAEIVTKNASVGNCLFPIQIVSVGQRLMTMNIVVFVTTVLVS 480

BLAST of Carg05274 vs. TrEMBL
Match: tr|A0A1Q3BMB7|A0A1Q3BMB7_CEPFO (Glyco_hydro_17 domain-containing protein/X8 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_12474 PE=3 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 9.6e-176
Identity = 292/480 (60.83%), Postives = 371/480 (77.29%), Query Frame = 0

Query: 4   ATIVPTAWAAVITVAAMAAGVAEGGIGVNWGTLASHPLKPSIVVNLLKDNGIKKVKLFDS 63
           A  V   WA    + +       G IGVNWGTLASHPL P+IVV +LKDNGI KVK+FD+
Sbjct: 2   ARAVYLLWAIFTLLGSANIVHGSGSIGVNWGTLASHPLPPNIVVGMLKDNGITKVKIFDA 61

Query: 64  DTWTVSSLAGSKIETIIGIPNDRLKKFAESYNEAKNWVKDNVTVHMYDGGVDIRYVSVGN 123
           D+WTVS+LAG+ IE ++GIPND+LK  A+SY + K+WVK+NVT H+Y+GGV+I+YV+VGN
Sbjct: 62  DSWTVSALAGTNIEVMVGIPNDQLKTLADSYGDCKDWVKENVTKHIYNGGVNIKYVAVGN 121

Query: 124 EAFLTAYNGSFVNVTFPAMQNVQKALDEAGHSKKIKVTTALNADVYEAKSNLPSDGQFRP 183
           E FLT+YNGS++N TFPA++N+QKAL+EAGH  +IK T  LNADVYE+ +N PSDG FR 
Sbjct: 122 EPFLTSYNGSYLNTTFPALKNIQKALNEAGHGDQIKATVPLNADVYESSTNQPSDGDFRS 181

Query: 184 DIYSIMKEIVRFLDRHDAPFMVNIYPFLSLYQNPNFPIDYAFFGGSGQSNNDNGKSYTNV 243
           DI  +M +IVRF   +DAPF+VNIYPFLSLY+N +FP ++AFFGG     ND G  Y NV
Sbjct: 182 DIKDLMLQIVRFFHDNDAPFVVNIYPFLSLYENDDFPFEFAFFGGGSNPLNDKGLQYNNV 241

Query: 244 FDANYDTLIWSLKKVGFPNLKIVIGEVGWPTDANVFANVTLAKRFYDGLFEKLASGEGTP 303
           FDANYDTL+WSLKK G PNLKIV+GEVGWPTD N   NV LA++FYDGL +KLAS +GTP
Sbjct: 242 FDANYDTLVWSLKKAGIPNLKIVVGEVGWPTDGNKNGNVKLAQKFYDGLLKKLASNKGTP 301

Query: 304 MRPKESFEVYLFGLLDENAKSVLPGFFERHWGIFQFDGKPKFPMDLSGKGNDKMLVAAKG 363
           +RP +  EVYLFGLLDE+ KS+ PGFFERHWGIF++DG+PKFP+D +G+G +KM + AKG
Sbjct: 302 LRPGK-LEVYLFGLLDEDLKSIAPGFFERHWGIFRYDGQPKFPIDFTGQGQEKMPIGAKG 361

Query: 364 VQYMERKWCVLRQTLKNLEPTAKEVDYACGYSDCTALTAGGSCGQLDRRGRISYAFNIYF 423
           VQY+  +WCVL + ++N      +VDYAC  SDCT+L+ G +C  LD  G +SYAFN+YF
Sbjct: 362 VQYLPSQWCVLNKAIRNTSEVPPQVDYACSLSDCTSLSYGSTCNNLDFVGNVSYAFNMYF 421

Query: 424 QMQDQNVEACVFDGTAEIVTKNATVGNCLFPIQIVSGGERLKAAAAAMV--GLVLSLFVL 482
           QMQDQ+V AC F G A++V++NA+ G CLFPIQIVS  ERL  A  A +  G+++S F+L
Sbjct: 422 QMQDQDVRACDFGGMAQLVSQNASKGTCLFPIQIVSASERLSFAYTATIAAGILVSFFML 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022963052.13.0e-282100.00glucan endo-1,3-beta-glucosidase 8-like [Cucurbita moschata][more]
XP_023003994.14.7e-27596.48glucan endo-1,3-beta-glucosidase 8-like [Cucurbita maxima][more]
XP_023518363.14.7e-27597.33glucan endo-1,3-beta-glucosidase 8-like [Cucurbita pepo subsp. pepo][more]
XP_008440124.14.0e-22677.48PREDICTED: glucan endo-1,3-beta-glucosidase 8 [Cucumis melo][more]
XP_004142041.11.2e-22578.05PREDICTED: glucan endo-1,3-beta-glucosidase 8 [Cucumis sativus] >KGN48417.1 hypo... [more]
Match NameE-valueIdentityDescription
AT5G64790.11.8e-15653.73O-Glycosyl hydrolases family 17 protein[more]
AT1G64760.15.0e-14653.66O-Glycosyl hydrolases family 17 protein[more]
AT2G19440.15.0e-14656.22O-Glycosyl hydrolases family 17 protein[more]
AT5G18220.11.6e-13955.01O-Glycosyl hydrolases family 17 protein[more]
AT3G04010.14.3e-13753.91O-Glycosyl hydrolases family 17 protein[more]
Match NameE-valueIdentityDescription
sp|Q6NKW9|E138_ARATH9.0e-14553.66Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ... [more]
sp|Q9M088|E135_ARATH6.1e-13349.89Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ... [more]
sp|Q93Z08|E136_ARATH4.4e-13150.56Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ... [more]
sp|Q9FGH4|E139_ARATH3.0e-9240.32Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ... [more]
sp|Q9M069|E137_ARATH5.8e-5935.08Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3B101|A0A1S3B101_CUCME2.7e-22677.48glucan endo-1,3-beta-glucosidase 8 OS=Cucumis melo OX=3656 GN=LOC103484682 PE=3 ... [more]
tr|A0A0A0KJ60|A0A0A0KJ60_CUCSA7.7e-22678.05Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G486900 PE=3 SV=1[more]
tr|A0A0A0LLR5|A0A0A0LLR5_CUCSA3.2e-19566.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G277010 PE=3 SV=1[more]
tr|A0A1S3C7C2|A0A1S3C7C2_CUCME2.1e-19466.32glucan endo-1,3-beta-glucosidase 8-like OS=Cucumis melo OX=3656 GN=LOC103497517 ... [more]
tr|A0A1Q3BMB7|A0A1Q3BMB7_CEPFO9.6e-17660.83Glyco_hydro_17 domain-containing protein/X8 domain-containing protein OS=Cephalo... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0004553hydrolase activity, hydrolyzing O-glycosyl compounds
Vocabulary: INTERPRO
TermDefinition
IPR017853Glycoside_hydrolase_SF
IPR000490Glyco_hydro_17
IPR012946X8
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05274-RACarg05274-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 370..453
e-value: 2.0E-28
score: 110.4
IPR012946X8 domainPFAMPF07983X8coord: 370..439
e-value: 1.3E-15
score: 57.7
NoneNo IPR availableGENE3DG3DSA:3.20.20.80coord: 28..357
e-value: 4.9E-117
score: 392.4
NoneNo IPR availablePANTHERPTHR32227:SF95BETA-1,3-GLUCANASE-LIKE PROTEIN-RELATEDcoord: 17..468
NoneNo IPR availablePANTHERPTHR32227FAMILY NOT NAMEDcoord: 17..468
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 29..348
e-value: 6.9E-74
score: 248.9
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 263..276
IPR017853Glycoside hydrolase superfamilySUPERFAMILYSSF51445(Trans)glycosidasescoord: 29..347