Carg05215 (gene) Silver-seed gourd

NameCarg05215
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionmitogen-activated protein kinase kinase kinase 3-like
LocationCucurbita_argyrosperma_scaffold_010 : 525739 .. 526803 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTGGATTCGAGGACATGAGATTGGGTGTGGGAGTTTTGCGACCATCAATTTAGCCATGCCTTCTAACACTTCTAATGCTCAGTTTCCTCCATTAATGGCCGTTAAATCGTCTCCAGCTGTCTCCTCTGCCTCGCTAAAGAACGAGAAGCGGGTTTTGGATCTTTTGGGTGATTGCCCTCAAATTATACGGTGTTTTGGTGAGAGCTGCAGCGTCGAGAGGGGCGACGAGTTGTATAATTTGTTTCTTGAGTATGCTAGTGGGGGTAGTTTGGCCGACAGAATACAGAGCCACGGTGGTTGGTTGCCGGAATTTGAGGTTCGGCGGTTGACGAGGACGATTCTCGAAGGGCTTCGGTATATTCACGACAAAGGATTTGTTCACTGCGATGTGAAGCCTCGGAATATTCTAGTGTTTGGTGACGGCGAGGCTAAAATTGCTGATTTTGGATTGTCGAAGAAGGCGGGGAAAAACAGAGCAGAAACCGGGGAAGAGGGGAAATTTCAATTGAGAGGCTCTCCCCTGTACATGTCGCCGGAATCGGTGAACGATAACGAGTACGAGTCGCCGTGTGACATTTGGGCTGTCGGATGCGCGGTGGTGGAGATGTTGACCGGAAAACCCGCTTGGAATTTCTCAATGGAATCCAACATCTATTCTCTGTTACTCAAAATTGGGATCGGAGAGGAATTGCCGAAAATCCCAAAATATATTTCCGAGGAAGGGAAAGATTTTCTAGGTAAATGCCTGGTGAAGGATCCCGCAAAGAGATGGACGGCTGAGATGCTTCTGAACCATCCATTTCTAGCAGACTCCGGCAGCAGCTCTGTTCCATTGACGCCCAAGGACGAACCATCAACGTCGCCGAGATGCCCTTTCGATTTCGAAGATTGGGCATCAATTCACTCGCAAGAGGTGGATCAGAAATTCGAGGAAGAATCAAATTGTTGGTTGGATTCGTCGTGTCCGTGGTCGTGTTCACCGACGGAGCGGCTGCTTCAGCTAGTGGACAACGGGCCAGTTGACTGGGTCATCACTGATAGTTGGGTCACCGTCAGATGA

mRNA sequence

ATGGACTGGATTCGAGGACATGAGATTGGGTGTGGGAGTTTTGCGACCATCAATTTAGCCATGCCTTCTAACACTTCTAATGCTCAGTTTCCTCCATTAATGGCCGTTAAATCGTCTCCAGCTGTCTCCTCTGCCTCGCTAAAGAACGAGAAGCGGGTTTTGGATCTTTTGGGTGATTGCCCTCAAATTATACGGTGTTTTGGTGAGAGCTGCAGCGTCGAGAGGGGCGACGAGTTGTATAATTTGTTTCTTGAGTATGCTAGTGGGGGTAGTTTGGCCGACAGAATACAGAGCCACGGTGGTTGGTTGCCGGAATTTGAGGTTCGGCGGTTGACGAGGACGATTCTCGAAGGGCTTCGGTATATTCACGACAAAGGATTTGTTCACTGCGATGTGAAGCCTCGGAATATTCTAGTGTTTGGTGACGGCGAGGCTAAAATTGCTGATTTTGGATTGTCGAAGAAGGCGGGGAAAAACAGAGCAGAAACCGGGGAAGAGGGGAAATTTCAATTGAGAGGCTCTCCCCTGTACATGTCGCCGGAATCGGTGAACGATAACGAGTACGAGTCGCCGTGTGACATTTGGGCTGTCGGATGCGCGGTGGTGGAGATGTTGACCGGAAAACCCGCTTGGAATTTCTCAATGGAATCCAACATCTATTCTCTGTTACTCAAAATTGGGATCGGAGAGGAATTGCCGAAAATCCCAAAATATATTTCCGAGGAAGGGAAAGATTTTCTAGGTAAATGCCTGGTGAAGGATCCCGCAAAGAGATGGACGGCTGAGATGCTTCTGAACCATCCATTTCTAGCAGACTCCGGCAGCAGCTCTGTTCCATTGACGCCCAAGGACGAACCATCAACGTCGCCGAGATGCCCTTTCGATTTCGAAGATTGGGCATCAATTCACTCGCAAGAGGTGGATCAGAAATTCGAGGAAGAATCAAATTGTTGGTTGGATTCGTCGTGTCCGTGGTCGTGTTCACCGACGGAGCGGCTGCTTCAGCTAGTGGACAACGGGCCAGTTGACTGGGTCATCACTGATAGTTGGGTCACCGTCAGATGA

Coding sequence (CDS)

ATGGACTGGATTCGAGGACATGAGATTGGGTGTGGGAGTTTTGCGACCATCAATTTAGCCATGCCTTCTAACACTTCTAATGCTCAGTTTCCTCCATTAATGGCCGTTAAATCGTCTCCAGCTGTCTCCTCTGCCTCGCTAAAGAACGAGAAGCGGGTTTTGGATCTTTTGGGTGATTGCCCTCAAATTATACGGTGTTTTGGTGAGAGCTGCAGCGTCGAGAGGGGCGACGAGTTGTATAATTTGTTTCTTGAGTATGCTAGTGGGGGTAGTTTGGCCGACAGAATACAGAGCCACGGTGGTTGGTTGCCGGAATTTGAGGTTCGGCGGTTGACGAGGACGATTCTCGAAGGGCTTCGGTATATTCACGACAAAGGATTTGTTCACTGCGATGTGAAGCCTCGGAATATTCTAGTGTTTGGTGACGGCGAGGCTAAAATTGCTGATTTTGGATTGTCGAAGAAGGCGGGGAAAAACAGAGCAGAAACCGGGGAAGAGGGGAAATTTCAATTGAGAGGCTCTCCCCTGTACATGTCGCCGGAATCGGTGAACGATAACGAGTACGAGTCGCCGTGTGACATTTGGGCTGTCGGATGCGCGGTGGTGGAGATGTTGACCGGAAAACCCGCTTGGAATTTCTCAATGGAATCCAACATCTATTCTCTGTTACTCAAAATTGGGATCGGAGAGGAATTGCCGAAAATCCCAAAATATATTTCCGAGGAAGGGAAAGATTTTCTAGGTAAATGCCTGGTGAAGGATCCCGCAAAGAGATGGACGGCTGAGATGCTTCTGAACCATCCATTTCTAGCAGACTCCGGCAGCAGCTCTGTTCCATTGACGCCCAAGGACGAACCATCAACGTCGCCGAGATGCCCTTTCGATTTCGAAGATTGGGCATCAATTCACTCGCAAGAGGTGGATCAGAAATTCGAGGAAGAATCAAATTGTTGGTTGGATTCGTCGTGTCCGTGGTCGTGTTCACCGACGGAGCGGCTGCTTCAGCTAGTGGACAACGGGCCAGTTGACTGGGTCATCACTGATAGTTGGGTCACCGTCAGATGA

Protein sequence

MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDCPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLRYIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKYISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWASIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR
BLAST of Carg05215 vs. NCBI nr
Match: XP_022962795.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata])

HSP 1 Score: 742.3 bits (1915), Expect = 7.8e-211
Identity = 351/354 (99.15%), Postives = 352/354 (99.44%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120
           PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120

Query: 121 YIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           YIH+KGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP
Sbjct: 121 YIHEKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKYIS 240
           ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNF MESNIYSLLLKIGIGEELPKIPKYIS
Sbjct: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFPMESNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300
           EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300

Query: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVD GPVDWVITDSWVTVR
Sbjct: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDKGPVDWVITDSWVTVR 354

BLAST of Carg05215 vs. NCBI nr
Match: XP_023522778.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo] >XP_023517683.1 mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 741.1 bits (1912), Expect = 1.7e-210
Identity = 350/354 (98.87%), Postives = 352/354 (99.44%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           MDWIRGHEIGCGSFATINLAMPSNTS AQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSTAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120
           PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120

Query: 121 YIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           YIH+KGFVHCDVKPRNILVFGDG+AKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP
Sbjct: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKYIS 240
           ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNF MESNIYSLLLKIGIGEELPKIPKYIS
Sbjct: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFPMESNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300
           EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300

Query: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR
Sbjct: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 354

BLAST of Carg05215 vs. NCBI nr
Match: XP_023003761.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita maxima])

HSP 1 Score: 726.1 bits (1873), Expect = 5.8e-206
Identity = 342/354 (96.61%), Postives = 348/354 (98.31%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           M+WIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC
Sbjct: 1   MEWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120
           PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRR TRTILEGLR
Sbjct: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEGLR 120

Query: 121 YIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           YIH+KGFVHCDVKPRNILVFGDG+AKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP
Sbjct: 121 YIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180

Query: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKYIS 240
           ESVND+EYESPCDIWAVGCAVVEMLTGKPAWNF +E NIYSLLLKIGIGEELPKIPKYIS
Sbjct: 181 ESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPKYIS 240

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300
           EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPK EP TSPRCPFDFEDWA
Sbjct: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFEDWA 300

Query: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           SIHS E+DQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR
Sbjct: 301 SIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 354

BLAST of Carg05215 vs. NCBI nr
Match: XP_008440288.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 3-like [Cucumis melo])

HSP 1 Score: 593.6 bits (1529), Expect = 4.5e-166
Identity = 291/357 (81.51%), Postives = 314/357 (87.96%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG-D 60
           MDW+RGHEIG GSFATINLA PS  S  +FPPLMAVKSS  V S+SLKNEK++LDLLG D
Sbjct: 1   MDWVRGHEIGHGSFATINLATPS--SGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDD 60

Query: 61  CPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGL 120
           CPQIIRCFGESCSVE G+ELYNLFLEYA+GGSLADRIQS GG LPEFEVRR TRTILEGL
Sbjct: 61  CPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGL 120

Query: 121 RYIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLYM 180
           RYIH KGFVHCDVKPRNILVFGDG+AKIADFGLSKKAGKNR ETGEE G+FQLRGSPLYM
Sbjct: 121 RYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYM 180

Query: 181 SPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKY 240
           SPES+NDNEYESPCDIWAVGCAVVEM TGKPAWN   ESN+++LL+KIGIGEELP+IPK 
Sbjct: 181 SPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKD 240

Query: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFED 300
           +SEEGKDFL KCLVKDP KRWTA+MLL HPF+ADSG   VPL   DE STSPRCPFDFED
Sbjct: 241 LSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSG-RCVPLGEMDEVSTSPRCPFDFED 300

Query: 301 WASIHSQEVDQKFEEESNCWLDSSCPW-SCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           WASIHSQE D + EEESNCWL+SSC W S SP ERLLQLV+ G VDW +TD+WV VR
Sbjct: 301 WASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 354

BLAST of Carg05215 vs. NCBI nr
Match: XP_011657865.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 3-like [Cucumis sativus] >KGN48531.1 hypothetical protein Csa_6G490950 [Cucumis sativus])

HSP 1 Score: 582.4 bits (1500), Expect = 1.0e-162
Identity = 286/356 (80.34%), Postives = 312/356 (87.64%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG-D 60
           MDW+RG EIG GSFATINLA  ++ S  +FPPLMAVKSS  V SASLKNEK+VLDLLG D
Sbjct: 1   MDWVRGDEIGYGSFATINLA--TSCSGDRFPPLMAVKSSGLVCSASLKNEKQVLDLLGDD 60

Query: 61  CPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGL 120
           CPQIIRCFG+SCSVE G+ELYNLFLEYASGGSLADRIQS GG LPEFEVRR TRTILEGL
Sbjct: 61  CPQIIRCFGDSCSVENGEELYNLFLEYASGGSLADRIQSRGGRLPEFEVRRYTRTILEGL 120

Query: 121 RYIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLYM 180
           RYIH KGFVHCDVKPRNILVFGDG+AKIADFGLSKKAGKNR  TGEE GKFQLRGSPLYM
Sbjct: 121 RYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVGTGEETGKFQLRGSPLYM 180

Query: 181 SPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKY 240
           SPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWN   ESN+++LL+KIGIGEELP+IPK 
Sbjct: 181 SPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNCRPESNVFALLIKIGIGEELPEIPKD 240

Query: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFED 300
           +SEEGKDFL KCLVKDP KRWTA+MLL HPF+A+SG   VPL   +E STSPRCPFDFED
Sbjct: 241 LSEEGKDFLKKCLVKDPLKRWTADMLLKHPFVAESG-RCVPLAGVEEVSTSPRCPFDFED 300

Query: 301 WASIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           WASIHSQE D + EEE+NCWL++   WSCSP ERLL+LV NG V+W +TD+WV VR
Sbjct: 301 WASIHSQESDPRNEEEANCWLNN---WSCSPRERLLELVGNGAVEWSVTDNWVRVR 350

BLAST of Carg05215 vs. TAIR10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19)

HSP 1 Score: 375.6 bits (963), Expect = 3.5e-104
Identity = 193/366 (52.73%), Postives = 245/366 (66.94%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG-D 60
           M+WIRG  IG G+F+T++LA  SN  + +FPPLMAVKS+ +  +ASL NEK VLD LG D
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDD 60

Query: 61  CPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGG-WLPEFEVRRLTRTILEG 120
           C +I+RCFGE  +VE G+E++NLFLEYAS GSL   ++   G  +PE  VRR T ++L G
Sbjct: 61  CNEIVRCFGEDRTVENGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLRG 120

Query: 121 LRYIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYM 180
           LR+IH  GF HCD+K  NIL+FGDG  KIADFGL+K+ G     T      Q+RG+PLYM
Sbjct: 121 LRHIHANGFAHCDLKLGNILLFGDGAVKIADFGLAKRIGD---LTALNYGVQIRGTPLYM 180

Query: 181 SPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKY 240
           +PESVNDNEY S  D+WA+GC VVEM +GK AW+    SN  SLLL+IG+G+E+P IP+ 
Sbjct: 181 APESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPEE 240

Query: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKD--------EPSTSP 300
           +SE+G+DFL KC VKDP KRWTAEMLLNHPF+       V +  +D        + STSP
Sbjct: 241 LSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFVVNMKTEDVSTSP 300

Query: 301 RCPFDFEDWASIH--SQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITD 355
           RCPF+F DW S+   SQ +D                   SP ER+  LV +   DW +T+
Sbjct: 301 RCPFEFPDWVSVSSGSQTID-------------------SPDERVASLVTDMIPDWSVTN 344

BLAST of Carg05215 vs. TAIR10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21)

HSP 1 Score: 372.9 bits (956), Expect = 2.3e-103
Identity = 193/357 (54.06%), Postives = 244/357 (68.35%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           M+WIR   IG GSF+T++LA  S +S+  FP LMAVKSS  V SA+L+NE+ VLD LGDC
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120
            +I+RCFGE  +VE G+E+YNLFLEYASGGSLADRI+S G  LPEFEVRR TR+I++GL 
Sbjct: 61  SEIVRCFGEGRTVENGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKGLC 120

Query: 121 YIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           +IH  GF HCD+K  N+LVFGDG+ KI+DFGL+K+          E   ++RG+PLYM+P
Sbjct: 121 HIHGNGFTHCDIKLENVLVFGDGDVKISDFGLAKR-------RSGEVCVEIRGTPLYMAP 180

Query: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAW--NFSMESNIYSLLLKIGIGEELPKIPKY 240
           ESVN  E+ESP DIWA+GC+VVEM +GK AW     + +N+ SLL++IG G+E+P+IP  
Sbjct: 181 ESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIPVE 240

Query: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEP-STSPRCPFDFE 300
           +SEEGKDF+ KC VK+ A+RWTAEMLL+HPFLA    S      +DE  S SPR PFDF 
Sbjct: 241 LSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDESG----EEDEACSVSPRNPFDFP 300

Query: 301 DWASIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
            W S+ S   D                   SP ER+  LV     DW ++  WV VR
Sbjct: 301 GWNSVQSPVNDSVMFGS----------LVGSPEERISGLVSEKVPDWSVSCDWVNVR 336

BLAST of Carg05215 vs. TAIR10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20)

HSP 1 Score: 369.8 bits (948), Expect = 1.9e-102
Identity = 192/361 (53.19%), Postives = 240/361 (66.48%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           M+W+RG  IG G+F+T++ A  S  S   FP L+AVKS+ A  +ASL NEK VLD LGDC
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNS-GDFPALIAVKSTDAYGAASLSNEKSVLDSLGDC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGG-WLPEFEVRRLTRTILEGL 120
           P+IIRC+GE  +VE G+E++NL LEYAS GSLA  ++  GG  LPE  VRR T ++L GL
Sbjct: 61  PEIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 120

Query: 121 RYIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMS 180
           R+IH KGF HCD+K  NIL+F DG  KIADFGL+ +   +   T      ++RG+PLYM+
Sbjct: 121 RHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDL--TALRKSVEIRGTPLYMA 180

Query: 181 PESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKYI 240
           PE VNDNEY S  D+WA+GCAVVEM +GK AW+    S+  SLL++IG+G+ELPKIP+ +
Sbjct: 181 PECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEML 240

Query: 241 SEEGKDFLGKCLVKDPAKRWTAEMLLNHPF----LADSGSSSVPLTPKDEPST--SPRCP 300
           SEEGKDFL KC VKDPAKRWTAEMLLNH F    L D    +  +  KDE     SP+CP
Sbjct: 241 SEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCP 300

Query: 301 FDFEDWASIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTV 355
           F+F+DW S         F  +SN   D       SP ERL  LV     DW +  SW+TV
Sbjct: 301 FEFDDWDS---------FTLDSNPSFD-------SPVERLGSLVSGSIPDWSVGGSWLTV 342

BLAST of Carg05215 vs. TAIR10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15)

HSP 1 Score: 234.6 bits (597), Expect = 9.6e-62
Identity = 140/361 (38.78%), Postives = 198/361 (54.85%), Query Frame = 0

Query: 2   DWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDCP 61
           +WIRG  IG GS AT++L +   T++  F    AVKS+   SSA L+ E+ +L  L   P
Sbjct: 5   NWIRGPIIGRGSTATVSLGI---TNSGDF---FAVKSAEFSSSAFLQREQSILSKLSS-P 64

Query: 62  QIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLRY 121
            I++  G + + E    +YNL +EY SGGSL D I++ GG LPE  +R  TR IL+GL Y
Sbjct: 65  YIVKYIGSNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMY 124

Query: 122 IHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSPE 181
           +HD+G VHCDVK +N+++ G+  AKI D G +K   +N          +  G+P +MSPE
Sbjct: 125 LHDQGIVHCDVKSQNVMIGGE-IAKIVDLGCAKTVEENE-------NLEFSGTPAFMSPE 184

Query: 182 SVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKYISE 241
                E   P D+WA+GC V+EM TG   W     +++ + + KIG   E P IP ++SE
Sbjct: 185 VARGEEQSFPADVWALGCTVIEMATGSSPW--PELNDVVAAIYKIGFTGESPVIPVWLSE 244

Query: 242 EGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDW-- 301
           +G+DFL KCL KDP +RWT E LL HPFL +  + S   T     S+SP    D   W  
Sbjct: 245 KGQDFLRKCLRKDPKQRWTVEELLQHPFLDEEDNDS-DQTGNCLNSSSPSTVLDQRFWDL 304

Query: 302 -----ASIHSQEVDQKFEEESN-CWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTV 355
                +    ++ +  F   +N  W D S P      ++L     +G  DW  T+ W+ V
Sbjct: 305 CETSRSRFIKEDHEDPFANSTNFLWDDDSLPG--DRIKKLAGDESSGEPDWE-TNGWIEV 344

BLAST of Carg05215 vs. TAIR10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16)

HSP 1 Score: 226.9 bits (577), Expect = 2.0e-59
Identity = 131/359 (36.49%), Postives = 194/359 (54.04%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           ++W RG  IG GS AT+++A+ S+        L AVKS+   SS+ L+ E+ +L  L   
Sbjct: 3   INWTRGPIIGRGSTATVSIAISSSGE------LFAVKSADLSSSSLLQKEQSILSTLSS- 62

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120
           P +++  G   + E    +YN+ +EY SGG+L D I++ GG LPE E+R  TR IL GL 
Sbjct: 63  PHMVKYIGTGLTRESNGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLV 122

Query: 121 YIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           Y+H++G VHCD+K  N+LV  +G  KIAD G +K   K+          +  G+P +M+P
Sbjct: 123 YLHERGIVHCDLKSHNVLVEENGVLKIADMGCAKSVDKS----------EFSGTPAFMAP 182

Query: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKYIS 240
           E     E   P D+WA+GC ++EM+TG   W     +++ + + KIG   E P IP +IS
Sbjct: 183 EVARGEEQRFPADVWALGCTMIEMMTGSSPW--PELNDVVAAMYKIGFSGESPAIPAWIS 242

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300
           ++ KDFL  CL +D  +RWT E LL HPFL D   S      K++ S SP    D   W 
Sbjct: 243 DKAKDFLKNCLKEDQKQRWTVEELLKHPFLDDDEESQTSDCLKNKTS-SPSTVLDQRFWD 302

Query: 301 SIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVD---NGPVDWVITD--SWVTVR 355
           S  S +      +  + + + S     SP +R+ +L     +  +DW   D   W+ VR
Sbjct: 303 SCESSKSHLVSIDHEDPFAEYSESLD-SPADRIEKLAGDEFSSLLDWDTEDDGGWIQVR 340

BLAST of Carg05215 vs. Swiss-Prot
Match: sp|O80888|M3K17_ARATH (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.5e-53
Identity = 132/361 (36.57%), Postives = 179/361 (49.58%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           M+W RG  +G GS AT+  A   N+       ++AVKSS    S  L+ E ++L  L   
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDE-----ILAVKSSEVHRSEFLQREAKILSSLSS- 60

Query: 61  PQIIRCFGESCSVE-RGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGL 120
           P +I   G     E  G  +YNL +EYA  G+L D     GG + E  V + TR IL+GL
Sbjct: 61  PYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGL 120

Query: 121 RYIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMS 180
            YIH KG VHCDVK  N+++   GEAKIADFG +K+            +  + G+P +M+
Sbjct: 121 EYIHSKGIVHCDVKGSNVVISEKGEAKIADFGCAKR-------VDPVFESPVMGTPAFMA 180

Query: 181 PESVNDNEYESPCDIWAVGCAVVEMLTGKPAW-NFSMESNIYSLLLKIGIGEELPKIPKY 240
           PE     +     DIWAVGC ++EM+TG P W       +  S+L ++G   E P++P  
Sbjct: 181 PEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCL 240

Query: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFED 300
           ++EE KDFL KCL ++  +RWTA  LLNHPFL         L P    S SP    D   
Sbjct: 241 LAEEAKDFLEKCLKREANERWTATQLLNHPFLTTKPDIEPVLVP-GLISNSPTSVTDQTF 300

Query: 301 WASIHSQEVDQKFEEESNCW-LDSSCPWSCSPTERLLQLVDNGPVDWVITD----SWVTV 355
           W S+                 LD    W C  +ER+ +L   G +D    D     W+ V
Sbjct: 301 WRSVEXXXXXXXXXXXXXXRDLDRLSLWGCY-SERIGRLKCVGGLDGTRCDMEGGDWIMV 346

BLAST of Carg05215 vs. Swiss-Prot
Match: sp|Q9ZVP5|M3K18_ARATH (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 2.3e-52
Identity = 134/367 (36.51%), Postives = 191/367 (52.04%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           M+W RG  +G GS AT++ A    +        +AVKS+    S  L+ E ++L  L + 
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGET-----LAVKSAEFHRSEFLQREAKILSSL-NS 60

Query: 61  PQIIRCFGESCSVER------GD-ELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTR 120
           P +I   G  C + R      G+   Y+L +EYA  G+L D    +GG++ E  V + TR
Sbjct: 61  PYVIGYRG--CEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTR 120

Query: 121 TILEGLRYIHD-KGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLR 180
            IL GL YIH+ KG  HCD+K  N+LV  +GEAKIADFG +K       E        +R
Sbjct: 121 QILLGLEYIHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITE-------PVR 180

Query: 181 GSPLYMSPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEEL 240
           G+P +M+PE+          DIWAVGC V+EM+TG   W  +  ++  S+L ++G   EL
Sbjct: 181 GTPAFMAPEAARGERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGEL 240

Query: 241 PKIPKYISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRC 300
           P++P  ++E+ KDFLGKCL K+  +RWTA  LLNHPFL     +  P       + SP  
Sbjct: 241 PELPCSLTEQAKDFLGKCLKKEATERWTASQLLNHPFLV----NKEPELVTGLVTNSPTS 300

Query: 301 PFDFEDWASIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPV----DWVIT- 355
             D   W S     V+++  E+ + W      W C   ER+  L   G V     W +  
Sbjct: 301 VTDQMFWRS-----VEEEVSEDRSSW------WECHEDERIGVLSWIGHVVVESTWDLDG 337

BLAST of Carg05215 vs. Swiss-Prot
Match: sp|Q40541|NPK1_TOBAC (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 4.7e-42
Identity = 104/288 (36.11%), Postives = 151/288 (52.43%), Query Frame = 0

Query: 3   WIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKS-SPAVSSAS----------LKNEK 62
           W +G  IGCG+F  + + M     N     L+A+K  S A++ AS          L+ E 
Sbjct: 80  WRKGEMIGCGAFGRVYMGM-----NVDSGELLAIKEVSIAMNGASRERAQAHVRELEEEV 139

Query: 63  RVLDLLGDCPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRL 122
            +L  L   P I+R  G +    R     N+ LE+  GGS++  +   G + PE  +R  
Sbjct: 140 NLLKNLSH-PNIVRYLGTA----REAGSLNILLEFVPGGSISSLLGKFGSF-PESVIRMY 199

Query: 123 TRTILEGLRYIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQL 182
           T+ +L GL Y+H  G +H D+K  NILV   G  K+ADFG SKK  +    TG +    +
Sbjct: 200 TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAK---SM 259

Query: 183 RGSPLYMSPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEE 242
           +G+P +M+PE +    +    DIW+VGC ++EM TGKP W  S +    + L  IG  + 
Sbjct: 260 KGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPW--SQQYQEVAALFHIGTTKS 319

Query: 243 LPKIPKYISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVP 280
            P IP+++S E KDFL KCL K+P  R +A  LL HPF+      + P
Sbjct: 320 HPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQEARP 351

BLAST of Carg05215 vs. Swiss-Prot
Match: sp|F4HRJ4|M3K3A_ARATH (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 8.1e-42
Identity = 99/290 (34.14%), Postives = 153/290 (52.76%), Query Frame = 0

Query: 3   WIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC-- 62
           W +G  +G G+F  + L   S          + V S    S   LK   + ++LL     
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKECLKQLNQEINLLNQLCH 273

Query: 63  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 122
           P I++ +G     E  +E  +++LEY SGGS+   ++ +G +  E  ++  TR IL GL 
Sbjct: 274 PNIVQYYGS----ELSEETLSVYLEYVSGGSIHKLLKDYGSF-TEPVIQNYTRQILAGLA 333

Query: 123 YIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 182
           Y+H +  VH D+K  NILV  +GE K+ADFG++K        T        +GSP +M+P
Sbjct: 334 YLHGRNTVHRDIKGANILVDPNGEIKLADFGMAKHV------TAFSTMLSFKGSPYWMAP 393

Query: 183 ESV-NDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKYI 242
           E V + N Y    DIW++GC ++EM T KP W+   +    + + KIG  ++ P+IP ++
Sbjct: 394 EVVMSQNGYTHAVDIWSLGCTILEMATSKPPWS---QFEGVAAIFKIGNSKDTPEIPDHL 453

Query: 243 SEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTS 290
           S + K+F+  CL ++P  R TA  LL HPFL ++   +    PKD P  S
Sbjct: 454 SNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRNTTRVASTSLPKDFPPRS 489

BLAST of Carg05215 vs. Swiss-Prot
Match: sp|Q9CAD5|YODA_ARATH (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 3.1e-41
Identity = 99/271 (36.53%), Postives = 145/271 (53.51%), Query Frame = 0

Query: 3   WIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC-- 62
           W +G  +G GSF  + L   S +        + + S    S  S +   + + +L     
Sbjct: 400 WKKGRLLGMGSFGHVYLGFNSESGEMCAMKEVTLCSDDPKSRESAQQLGQEISVLSRLRH 459

Query: 63  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 122
             I++ +G     E  D+   ++LEY SGGS+   +Q +G +  E  +R  T+ IL GL 
Sbjct: 460 QNIVQYYGS----ETVDDKLYIYLEYVSGGSIYKLLQEYGQF-GENAIRNYTQQILSGLA 519

Query: 123 YIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 182
           Y+H K  VH D+K  NILV   G  K+ADFG++K        T + G    +GSP +M+P
Sbjct: 520 YLHAKNTVHRDIKGANILVDPHGRVKVADFGMAKHI------TAQSGPLSFKGSPYWMAP 579

Query: 183 ESV-NDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKYI 242
           E + N N      DIW++GC V+EM T KP W+   +      + KIG  +ELP IP ++
Sbjct: 580 EVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWS---QYEGVPAMFKIGNSKELPDIPDHL 639

Query: 243 SEEGKDFLGKCLVKDPAKRWTAEMLLNHPFL 271
           SEEGKDF+ KCL ++PA R TA  LL+H F+
Sbjct: 640 SEEGKDFVRKCLQRNPANRPTAAQLLDHAFV 656

BLAST of Carg05215 vs. TrEMBL
Match: tr|A0A1S3B1I0|A0A1S3B1I0_CUCME (mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo OX=3656 GN=LOC103484781 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 3.0e-166
Identity = 291/357 (81.51%), Postives = 314/357 (87.96%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG-D 60
           MDW+RGHEIG GSFATINLA PS  S  +FPPLMAVKSS  V S+SLKNEK++LDLLG D
Sbjct: 1   MDWVRGHEIGHGSFATINLATPS--SGDRFPPLMAVKSSELVCSSSLKNEKQILDLLGDD 60

Query: 61  CPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGL 120
           CPQIIRCFGESCSVE G+ELYNLFLEYA+GGSLADRIQS GG LPEFEVRR TRTILEGL
Sbjct: 61  CPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEGL 120

Query: 121 RYIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLYM 180
           RYIH KGFVHCDVKPRNILVFGDG+AKIADFGLSKKAGKNR ETGEE G+FQLRGSPLYM
Sbjct: 121 RYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLYM 180

Query: 181 SPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKY 240
           SPES+NDNEYESPCDIWAVGCAVVEM TGKPAWN   ESN+++LL+KIGIGEELP+IPK 
Sbjct: 181 SPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPKD 240

Query: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFED 300
           +SEEGKDFL KCLVKDP KRWTA+MLL HPF+ADSG   VPL   DE STSPRCPFDFED
Sbjct: 241 LSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSG-RCVPLGEMDEVSTSPRCPFDFED 300

Query: 301 WASIHSQEVDQKFEEESNCWLDSSCPW-SCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           WASIHSQE D + EEESNCWL+SSC W S SP ERLLQLV+ G VDW +TD+WV VR
Sbjct: 301 WASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 354

BLAST of Carg05215 vs. TrEMBL
Match: tr|A0A0A0KLC9|A0A0A0KLC9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490950 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 6.8e-163
Identity = 286/356 (80.34%), Postives = 312/356 (87.64%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG-D 60
           MDW+RG EIG GSFATINLA  ++ S  +FPPLMAVKSS  V SASLKNEK+VLDLLG D
Sbjct: 1   MDWVRGDEIGYGSFATINLA--TSCSGDRFPPLMAVKSSGLVCSASLKNEKQVLDLLGDD 60

Query: 61  CPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGL 120
           CPQIIRCFG+SCSVE G+ELYNLFLEYASGGSLADRIQS GG LPEFEVRR TRTILEGL
Sbjct: 61  CPQIIRCFGDSCSVENGEELYNLFLEYASGGSLADRIQSRGGRLPEFEVRRYTRTILEGL 120

Query: 121 RYIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLYM 180
           RYIH KGFVHCDVKPRNILVFGDG+AKIADFGLSKKAGKNR  TGEE GKFQLRGSPLYM
Sbjct: 121 RYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVGTGEETGKFQLRGSPLYM 180

Query: 181 SPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKY 240
           SPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWN   ESN+++LL+KIGIGEELP+IPK 
Sbjct: 181 SPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNCRPESNVFALLIKIGIGEELPEIPKD 240

Query: 241 ISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFED 300
           +SEEGKDFL KCLVKDP KRWTA+MLL HPF+A+SG   VPL   +E STSPRCPFDFED
Sbjct: 241 LSEEGKDFLKKCLVKDPLKRWTADMLLKHPFVAESG-RCVPLAGVEEVSTSPRCPFDFED 300

Query: 301 WASIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 355
           WASIHSQE D + EEE+NCWL++   WSCSP ERLL+LV NG V+W +TD+WV VR
Sbjct: 301 WASIHSQESDPRNEEEANCWLNN---WSCSPRERLLELVGNGAVEWSVTDNWVRVR 350

BLAST of Carg05215 vs. TrEMBL
Match: tr|A0A2C9USJ9|A0A2C9USJ9_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_13G123400 PE=3 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 2.4e-115
Identity = 203/359 (56.55%), Postives = 267/359 (74.37%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           MDW+RG  IGCGSF+T+NLA+P+ TS+    PLMAVKS     S+ L+NEK VL+ LG+C
Sbjct: 1   MDWVRGDSIGCGSFSTVNLAIPTKTSS----PLMAVKSCDLFDSSLLENEKDVLNQLGNC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120
             II+CFG   SVE G  LYNL LEYA GGSL+  +++ GG LPE + RR T++IL+GLR
Sbjct: 61  EHIIKCFGGDQSVENGQRLYNLLLEYAPGGSLSRLVKNSGGCLPESDARRYTKSILKGLR 120

Query: 121 YIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           YIH KGF HCD+K +NIL+F DG+ KIADFGL+KK G+ +++  E+G+ ++RG+PLYMSP
Sbjct: 121 YIHAKGFAHCDIKLQNILLFEDGDVKIADFGLAKKTGEEQSD--EQGRVEIRGTPLYMSP 180

Query: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKYIS 240
           ESVN+NEY+SPCDIWA+GC +VEM+TGKPAWN   ESNI SLL++IG+G+ELP+IP+ +S
Sbjct: 181 ESVNENEYDSPCDIWALGCGLVEMVTGKPAWNCKPESNIASLLIRIGVGDELPEIPQELS 240

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFEDWA 300
           +E KDFL KC VKDP KRWTA++LLNHPF+A+  + ++    +  PS+SPRCPF+F +W 
Sbjct: 241 QEAKDFLSKCFVKDPRKRWTADLLLNHPFIANETTVTLKEKEQLSPSSSPRCPFEFPEWV 300

Query: 301 SIHSQEVDQKFEEESNCWLDSSCPWSC-----SPTERLLQLVDNGPVDWVITDSWVTVR 355
           S+ S     K E  SN  ++S   WS      SP ERL QL  +   +W  ++SWVTVR
Sbjct: 301 SVPSS--SPKSELWSNKEVESRFDWSSLSYSPSPAERLRQLGSDEGCNWSFSESWVTVR 351

BLAST of Carg05215 vs. TrEMBL
Match: tr|A0A2I4E2U8|A0A2I4E2U8_9ROSI (mitogen-activated protein kinase kinase kinase 3-like OS=Juglans regia OX=51240 GN=LOC108985758 PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 3.4e-114
Identity = 213/370 (57.57%), Postives = 270/370 (72.97%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           M+W+RG  +G GSFAT+NLA+P N S A   PLM VKSS +  S SLKNEK+VLD L  C
Sbjct: 23  MEWVRGEPVGHGSFATVNLAIPRNGS-ALASPLMVVKSSESSCSVSLKNEKQVLDQLSTC 82

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120
           PQ+IRC G+  SVE G+ELYNLFLEYAS GSLAD++Q+ GG LPE +VRR  +++L+GLR
Sbjct: 83  PQVIRCLGDDHSVENGEELYNLFLEYASHGSLADQLQNRGGSLPESDVRRCVKSVLKGLR 142

Query: 121 YIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
            +H KGFVHCD+K +NILVFG+G  KIADFGL+KKA   + ++GE  + + RG+PLYMSP
Sbjct: 143 DVHAKGFVHCDIKLQNILVFGNGAIKIADFGLAKKA--EQEQSGEGNRAEFRGTPLYMSP 202

Query: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKYIS 240
           ESVND+EYESP DIWA+GCAVVEMLTGKPAWN   E NI  LL++IG+G+ELP++PK +S
Sbjct: 203 ESVNDSEYESPADIWALGCAVVEMLTGKPAWNCGAEYNICKLLIRIGVGDELPQVPKELS 262

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLA---DSGSSSVPLTPKDE--PSTSPRCPFD 300
            EGKDFLGKC VKDP KRWTAEMLL+HPF+A   ++   ++PL  +DE   S+SPR PFD
Sbjct: 263 FEGKDFLGKCFVKDPRKRWTAEMLLDHPFVAAGDNNDHDAIPLEGRDESKTSSSPRSPFD 322

Query: 301 FEDWASIHSQEV---------DQKFE-EESNCWLDS-SCPWSCSPTERLLQLVDNGPVDW 355
           F +W S+ S  V          + FE E  N    S +    CSP +RL Q+  +   +W
Sbjct: 323 FPEWVSVQSSAVVSPESSPNSGKVFEWEVMNSGAGSLALSRFCSPMDRLRQMAADELPNW 382

BLAST of Carg05215 vs. TrEMBL
Match: tr|A0A2C9UWE2|A0A2C9UWE2_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_12G103600 PE=3 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.7e-113
Identity = 205/360 (56.94%), Postives = 266/360 (73.89%), Query Frame = 0

Query: 1   MDWIRGHEIGCGSFATINLAMPSNTSNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLGDC 60
           MDW RG  IGCGSF+T+NLA+P + S     PLMAVKS     S+ L+NEK VL  LG+C
Sbjct: 1   MDWFRGDCIGCGSFSTVNLAIPRSIS----APLMAVKSCDVFESSLLENEKDVLTQLGNC 60

Query: 61  PQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEGLR 120
             +I+CFG   SVE G  LYNL LEYASGGSL+  ++  GG LPE + RR TR+IL+GL 
Sbjct: 61  EHVIQCFGGDQSVENGKMLYNLLLEYASGGSLSHLVKKSGGCLPESDARRYTRSILKGLH 120

Query: 121 YIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEEGKFQLRGSPLYMSP 180
           YIH KGF HCD+K +NIL+F +GEAKIADFGL+KK G+   ++GE+G+ + RG+PLYMSP
Sbjct: 121 YIHAKGFAHCDIKLQNILLFENGEAKIADFGLAKKTGQK--QSGEQGRIEFRGTPLYMSP 180

Query: 181 ESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPKYIS 240
           ESVN+NEY+SPCDIWA+GCA+VEM+TGKPAWN   E+N+ +LL++IG+G+ELP+IP+ +S
Sbjct: 181 ESVNENEYDSPCDIWALGCALVEMVTGKPAWNCKQETNVAALLIRIGVGDELPEIPQELS 240

Query: 241 EEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPK-DEPSTSPRCPFDFEDW 300
           +E KDFL KC VKDP +RWTA+MLL+HPF+A+   ++VPLT +   PS+SPRCPF+F +W
Sbjct: 241 KEAKDFLSKCFVKDPKRRWTADMLLDHPFVAN--ETTVPLTEQFSPPSSSPRCPFEFPEW 300

Query: 301 ASIHSQEVDQKFEEESNCWLDSSCPWSC-----SPTERLLQLVDNGPVDWVITDSWVTVR 355
            S+ S     K E  SN  ++S   WS      SP ERL QL  +   +W  ++SWVTVR
Sbjct: 301 VSVPSS--SPKSELWSNKEVESRFDWSSLSYSPSPAERLRQLGSDQGCNWSFSESWVTVR 350

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022962795.17.8e-21199.15mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata][more]
XP_023522778.11.7e-21098.87mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pe... [more]
XP_023003761.15.8e-20696.61mitogen-activated protein kinase kinase kinase 18-like [Cucurbita maxima][more]
XP_008440288.14.5e-16681.51PREDICTED: mitogen-activated protein kinase kinase kinase 3-like [Cucumis melo][more]
XP_011657865.11.0e-16280.34PREDICTED: mitogen-activated protein kinase kinase kinase 3-like [Cucumis sativu... [more]
Match NameE-valueIdentityDescription
AT5G67080.13.5e-10452.73mitogen-activated protein kinase kinase kinase 19[more]
AT4G36950.12.3e-10354.06mitogen-activated protein kinase kinase kinase 21[more]
AT3G50310.11.9e-10253.19mitogen-activated protein kinase kinase kinase 20[more]
AT5G55090.19.6e-6238.78mitogen-activated protein kinase kinase kinase 15[more]
AT4G26890.12.0e-5936.49mitogen-activated protein kinase kinase kinase 16[more]
Match NameE-valueIdentityDescription
sp|O80888|M3K17_ARATH3.5e-5336.57Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
sp|Q9ZVP5|M3K18_ARATH2.3e-5236.51Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
sp|Q40541|NPK1_TOBAC4.7e-4236.11Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
sp|F4HRJ4|M3K3A_ARATH8.1e-4234.14Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
sp|Q9CAD5|YODA_ARATH3.1e-4136.53Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3B1I0|A0A1S3B1I0_CUCME3.0e-16681.51mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo OX=3656 GN... [more]
tr|A0A0A0KLC9|A0A0A0KLC9_CUCSA6.8e-16380.34Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490950 PE=3 SV=1[more]
tr|A0A2C9USJ9|A0A2C9USJ9_MANES2.4e-11556.55Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_13G123400 PE=3 SV=... [more]
tr|A0A2I4E2U8|A0A2I4E2U8_9ROSI3.4e-11457.57mitogen-activated protein kinase kinase kinase 3-like OS=Juglans regia OX=51240 ... [more]
tr|A0A2C9UWE2|A0A2C9UWE2_MANES1.7e-11356.94Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_12G103600 PE=3 SV=... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004672protein kinase activity
GO:0005524ATP binding
Vocabulary: Biological Process
TermDefinition
GO:0006468protein phosphorylation
Vocabulary: INTERPRO
TermDefinition
IPR011009Kinase-like_dom_sf
IPR008271Ser/Thr_kinase_AS
IPR017441Protein_kinase_ATP_BS
IPR000719Prot_kinase_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05215-RACarg05215-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 3..270
e-value: 3.8E-68
score: 242.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 6..270
e-value: 1.8E-54
score: 184.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 3..270
score: 39.594
NoneNo IPR availablePIRSFPIRSF000654ILKcoord: 45..267
e-value: 6.5E-20
score: 69.1
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 2..319
e-value: 8.6E-66
score: 224.4
NoneNo IPR availablePANTHERPTHR24361MITOGEN-ACTIVATED KINASE KINASE KINASEcoord: 1..303
NoneNo IPR availablePANTHERPTHR24361:SF334MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATEDcoord: 1..303
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 2..270
e-value: 7.90416E-117
score: 341.037
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 9..37
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 127..139
IPR011009Protein kinase-like domain superfamilySUPERFAMILYSSF56112Protein kinase-like (PK-like)coord: 3..294

The following gene(s) are paralogous to this gene:

None