Carg05163 (gene) Silver-seed gourd

NameCarg05163
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4
LocationCucurbita_argyrosperma_scaffold_010 : 220803 .. 222779 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCTTCAAAGCTGCCTCCTTCCACGACGGTGCTGAAATGGCTGGGATTTGTCTCCGCCGTCTGGGTTCAAGCCATTTCCGGCAACAACTACACCTTCTCCAACTACTCCGACGCCCTCAAATCTCTCATGAGTCTTACCCAACTTCAGCTCAACAATCTCTCCGTCGCTAAAGACGTCGGTAAAGCCTTCGGTCTTCTCGCCGGAATCGCCTCTGATCGGTTTCCGACATGGGTCATCTTGCTCATCGGATCCCTTGAAGGTCTCATTGGCTATGGCGCTCAATGGCTCGTCGTTAGCCAGAGAATTTCCCCTCTTCCTTACTGGCAGGTATTTCTCTTTACAATGAATTTGAATTAGTTCTTGATTGCGTAGCGGTTTCAATTTCACTGAAAAACATGGTTGTCCGTTGAAACAGATGTGTTTCTTTCTGTGTTTGGGCGGAAACAGTACCACGTGGATGAACACGGCGGTTCTGGTTACCTGTATCAGAAACTTCCGGAAGAATCGCGGCCCGGTTTCCGGTATTCTCAAAGGCTATGTCGGCCTTAGTACCGCCATTTTCACTGACCTCTGTACTGCTCTGTTTTCCGATGACCCATCTTCCTTCCTCGTCATGCTCGCCGTCGTCCCCTTTGCCGTCTGTCTCTTCGCCATGTTCTTCCTCCGTGAGATTCCGTCTACAGCAACCGCCAACGCCCAAGAAGAGTCCAGTTTCTTCGCCGTCTTTAACGCCCTCGCCGTCGTCGTCGCCGTCTATCTCTTAGGCTTCGATTTCATCAAAAACTCCGGCAAAGTCGTTTCCCAGTTGTTCTCTGTCGGATTATTGATTCTCCTCGGATCCCCTTTGGTTATTCCGATTTACTCGTTGTTCAAGAGCTGGAATTCGAATCAAACCCAATTGGATTTAGAGGCTAAAATTGAGGAGCCATTACTTAAGGAAGAAGCCGCGGCGGCGGCGGTTGAACAGAGTACGGTGGTGAAGGAGGAAGAAATAACAGAGGAGAGAACACCGCCGGTGATCGGCGAAGAACACACGATTTTTGAAGCGATGAAAACGGTGGATTTCTGGGTTTTGTTCGTGTCGTTTCTCTGCGGCGTGGGGACAGGATTGGCGGTGATGAACAACATGGGTCAGATCGGATTAGCTCTTGGTTACGCCGATGTTTCCATGTTCGTTTCTCTGACAAGCATCTGGGGCTTTTTCGGCCGGATTCTCGCCGGCACAATTTCCGAGCACTTTCTCAAGTAAGTTTCCAATTTCCAAACCGACAAAATTCAAATTCAATCCGACAAATCTAACAGAATCTTTATAAACATTTCAGAAAATCCGGCACACCGAGGCCTTTATGGAACGCCGCATCTCAAATTCTAATGACAATTGGATACATTCTAATGGCAATGGCAATGCCAGGGTCTCTCTACATCGGTTCCATGGTCGTCGGGATTTGCTACGGCGTACGTCTCTCCATCACCGTCCCCACCGCGTCGGAGCTTTTCGGGCTCAAATACTACGGTCTAATTTACAACATTCTGATCCTCAACCTCCCGATCGGTTCGTTCCTGTTCTCGGGGCTTCTCGCCGGGTTCCTCTACGACATGGAGGCGACACCGACCCCGGGCGGCGGGAACACGTGCATCGGTGGGCATTGTTACAGAATCGTGTTCGTTGTGATGGCTTTAGCTTGTGTGATTGGGTTTGTTTTGGACATTTGGTTGGCGTTTAGAACCAAGGTGCTTTATTCAAAGCTGAAAGCCAACAAGAAATCAAACAAAGGGAGCTCTTAGAGTGGGATGGTTTGGTAAATTGGTGGATTGATGGGAATTTTTACCCAATGATATCCCCAATTTTTTTTATTTTATATTATTTTGTCATTATAGTTTGGAAATTATTATTATTATTTTTAGTTTTATTGTTTAATTAATGTAACAAATTATCTAATTCTAGTCTATAAATTTTCACGTTTGTATTTA

mRNA sequence

ATGGGTTCTTCAAAGCTGCCTCCTTCCACGACGGTGCTGAAATGGCTGGGATTTGTCTCCGCCGTCTGGGTTCAAGCCATTTCCGGCAACAACTACACCTTCTCCAACTACTCCGACGCCCTCAAATCTCTCATGAGTCTTACCCAACTTCAGCTCAACAATCTCTCCGTCGCTAAAGACGTCGGTAAAGCCTTCGGTCTTCTCGCCGGAATCGCCTCTGATCGGTTTCCGACATGGGTCATCTTGCTCATCGGATCCCTTGAAGGTCTCATTGGCTATGGCGCTCAATGGCTCGTCGTTAGCCAGAGAATTTCCCCTCTTCCTTACTGGCAGATGTGTTTCTTTCTGTGTTTGGGCGGAAACAGTACCACGTGGATGAACACGGCGGTTCTGGTTACCTGTATCAGAAACTTCCGGAAGAATCGCGGCCCGGTTTCCGGTATTCTCAAAGGCTATGTCGGCCTTAGTACCGCCATTTTCACTGACCTCTGTACTGCTCTGTTTTCCGATGACCCATCTTCCTTCCTCGTCATGCTCGCCGTCGTCCCCTTTGCCGTCTGTCTCTTCGCCATGTTCTTCCTCCGTGAGATTCCGTCTACAGCAACCGCCAACGCCCAAGAAGAGTCCAGTTTCTTCGCCGTCTTTAACGCCCTCGCCGTCGTCGTCGCCGTCTATCTCTTAGGCTTCGATTTCATCAAAAACTCCGGCAAAGTCGTTTCCCAGTTGTTCTCTGTCGGATTATTGATTCTCCTCGGATCCCCTTTGGTTATTCCGATTTACTCGTTGTTCAAGAGCTGGAATTCGAATCAAACCCAATTGGATTTAGAGGCTAAAATTGAGGAGCCATTACTTAAGGAAGAAGCCGCGGCGGCGGCGGTTGAACAGAGTACGGTGGTGAAGGAGGAAGAAATAACAGAGGAGAGAACACCGCCGGTGATCGGCGAAGAACACACGATTTTTGAAGCGATGAAAACGGTGGATTTCTGGGTTTTGTTCGTGTCGTTTCTCTGCGGCGTGGGGACAGGATTGGCGGTGATGAACAACATGGGTCAGATCGGATTAGCTCTTGGTTACGCCGATGTTTCCATGTTCGTTTCTCTGACAAGCATCTGGGGCTTTTTCGGCCGGATTCTCGCCGGCACAATTTCCGAGCACTTTCTCAAAAAATCCGGCACACCGAGGCCTTTATGGAACGCCGCATCTCAAATTCTAATGACAATTGGATACATTCTAATGGCAATGGCAATGCCAGGGTCTCTCTACATCGGTTCCATGGTCGTCGGGATTTGCTACGGCGTACGTCTCTCCATCACCGTCCCCACCGCGTCGGAGCTTTTCGGGCTCAAATACTACGGTCTAATTTACAACATTCTGATCCTCAACCTCCCGATCGGTTCGTTCCTGTTCTCGGGGCTTCTCGCCGGGTTCCTCTACGACATGGAGGCGACACCGACCCCGGGCGGCGGGAACACGTGCATCGGTGGGCATTGTTACAGAATCGTGTTCGTTGTGATGGCTTTAGCTTGTGTGATTGGGTTTGTTTTGGACATTTGGTTGGCGTTTAGAACCAAGGTGCTTTATTCAAAGCTGAAAGCCAACAAGAAATCAAACAAAGGGAGCTCTTAGAGTGGGATGGTTTGGTAAATTGGTGGATTGATGGGAATTTTTACCCAATGATATCCCCAATTTTTTTTATTTTATATTATTTTGTCATTATAGTTTGGAAATTATTATTATTATTTTTAGTTTTATTGTTTAATTAATGTAACAAATTATCTAATTCTAGTCTATAAATTTTCACGTTTGTATTTA

Coding sequence (CDS)

ATGGGTTCTTCAAAGCTGCCTCCTTCCACGACGGTGCTGAAATGGCTGGGATTTGTCTCCGCCGTCTGGGTTCAAGCCATTTCCGGCAACAACTACACCTTCTCCAACTACTCCGACGCCCTCAAATCTCTCATGAGTCTTACCCAACTTCAGCTCAACAATCTCTCCGTCGCTAAAGACGTCGGTAAAGCCTTCGGTCTTCTCGCCGGAATCGCCTCTGATCGGTTTCCGACATGGGTCATCTTGCTCATCGGATCCCTTGAAGGTCTCATTGGCTATGGCGCTCAATGGCTCGTCGTTAGCCAGAGAATTTCCCCTCTTCCTTACTGGCAGATGTGTTTCTTTCTGTGTTTGGGCGGAAACAGTACCACGTGGATGAACACGGCGGTTCTGGTTACCTGTATCAGAAACTTCCGGAAGAATCGCGGCCCGGTTTCCGGTATTCTCAAAGGCTATGTCGGCCTTAGTACCGCCATTTTCACTGACCTCTGTACTGCTCTGTTTTCCGATGACCCATCTTCCTTCCTCGTCATGCTCGCCGTCGTCCCCTTTGCCGTCTGTCTCTTCGCCATGTTCTTCCTCCGTGAGATTCCGTCTACAGCAACCGCCAACGCCCAAGAAGAGTCCAGTTTCTTCGCCGTCTTTAACGCCCTCGCCGTCGTCGTCGCCGTCTATCTCTTAGGCTTCGATTTCATCAAAAACTCCGGCAAAGTCGTTTCCCAGTTGTTCTCTGTCGGATTATTGATTCTCCTCGGATCCCCTTTGGTTATTCCGATTTACTCGTTGTTCAAGAGCTGGAATTCGAATCAAACCCAATTGGATTTAGAGGCTAAAATTGAGGAGCCATTACTTAAGGAAGAAGCCGCGGCGGCGGCGGTTGAACAGAGTACGGTGGTGAAGGAGGAAGAAATAACAGAGGAGAGAACACCGCCGGTGATCGGCGAAGAACACACGATTTTTGAAGCGATGAAAACGGTGGATTTCTGGGTTTTGTTCGTGTCGTTTCTCTGCGGCGTGGGGACAGGATTGGCGGTGATGAACAACATGGGTCAGATCGGATTAGCTCTTGGTTACGCCGATGTTTCCATGTTCGTTTCTCTGACAAGCATCTGGGGCTTTTTCGGCCGGATTCTCGCCGGCACAATTTCCGAGCACTTTCTCAAAAAATCCGGCACACCGAGGCCTTTATGGAACGCCGCATCTCAAATTCTAATGACAATTGGATACATTCTAATGGCAATGGCAATGCCAGGGTCTCTCTACATCGGTTCCATGGTCGTCGGGATTTGCTACGGCGTACGTCTCTCCATCACCGTCCCCACCGCGTCGGAGCTTTTCGGGCTCAAATACTACGGTCTAATTTACAACATTCTGATCCTCAACCTCCCGATCGGTTCGTTCCTGTTCTCGGGGCTTCTCGCCGGGTTCCTCTACGACATGGAGGCGACACCGACCCCGGGCGGCGGGAACACGTGCATCGGTGGGCATTGTTACAGAATCGTGTTCGTTGTGATGGCTTTAGCTTGTGTGATTGGGTTTGTTTTGGACATTTGGTTGGCGTTTAGAACCAAGGTGCTTTATTCAAAGCTGAAAGCCAACAAGAAATCAAACAAAGGGAGCTCTTAG

Protein sequence

MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKDVGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGGNSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLAVVPFAVCLFAMFFLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVSQLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLLKEEAAAAAVEQSTVVKEEEITEERTPPVIGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSLYIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNKGSS
BLAST of Carg05163 vs. NCBI nr
Match: XP_023532459.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 989.9 bits (2558), Expect = 3.3e-285
Identity = 540/541 (99.82%), Postives = 541/541 (100.00%), Query Frame = 0

Query: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKD 60
           MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLM+LTQLQLNNLSVAKD
Sbjct: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120
           VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG
Sbjct: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180

Query: 181 VVPFAVCLFAMFFLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVS 240
           VVPFAVCLFAMFFLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVS
Sbjct: 181 VVPFAVCLFAMFFLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVS 240

Query: 241 QLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLXXXXXXXXXXXXXXXXX 300
           QLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLXXXXXXXXXXXXXXXXX
Sbjct: 241 QLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLXXXXXXXXXXXXXXXXX 300

Query: 301 XXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360
           XXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD
Sbjct: 301 XXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360

Query: 361 VSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSL 420
           VSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSL
Sbjct: 361 VSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSL 420

Query: 421 YIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480
           YIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM
Sbjct: 421 YIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480

Query: 481 EATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNKGS 540
           EATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNKGS
Sbjct: 481 EATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNKGS 540

Query: 541 S 542
           S
Sbjct: 541 S 541

BLAST of Carg05163 vs. NCBI nr
Match: XP_022948188.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 987.3 bits (2551), Expect = 2.1e-284
Identity = 535/541 (98.89%), Postives = 535/541 (98.89%), Query Frame = 0

Query: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKD 60
           MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKD
Sbjct: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120
           VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG
Sbjct: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180

Query: 181 VVPFAVCLFAMFFLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVS 240
           VVPFAVCLFAMFFLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVS
Sbjct: 181 VVPFAVCLFAMFFLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVS 240

Query: 241 QLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLXXXXXXXXXXXXXXXXX 300
           QLFSVGLLILLGSPLVIPIYS  KSWNSNQTQLDLEAKIEEPL  XXXXXXXXXXXXXXX
Sbjct: 241 QLFSVGLLILLGSPLVIPIYSFIKSWNSNQTQLDLEAKIEEPLLKXXXXXXXXXXXXXXX 300

Query: 301 XXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360
           XXXXXXXXXXX  GEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD
Sbjct: 301 XXXXXXXXXXXVIGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360

Query: 361 VSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSL 420
           VSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSL
Sbjct: 361 VSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSL 420

Query: 421 YIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480
           YIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM
Sbjct: 421 YIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480

Query: 481 EATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNKGS 540
           EATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNKGS
Sbjct: 481 EATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNKGS 540

Query: 541 S 542
           S
Sbjct: 541 S 541

BLAST of Carg05163 vs. NCBI nr
Match: XP_022970978.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 981.9 bits (2537), Expect = 8.9e-283
Identity = 526/541 (97.23%), Postives = 528/541 (97.60%), Query Frame = 0

Query: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKD 60
           MGSSKLPPSTTVLKWLGFVSAVW+QAISGNNYTFSNYSDALKSLM+LTQLQLNNLSVAKD
Sbjct: 1   MGSSKLPPSTTVLKWLGFVSAVWIQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120
           VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG
Sbjct: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180

Query: 181 VVPFAVCLFAMFFLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVS 240
           VVPFAVCLFAMFFLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVS
Sbjct: 181 VVPFAVCLFAMFFLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVS 240

Query: 241 QLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLXXXXXXXXXXXXXXXXX 300
           QLFSVGLLILLGSPLVIPIYS FKSWNSNQTQLDLEAKIEEPL      XXXXXXXXXXX
Sbjct: 241 QLFSVGLLILLGSPLVIPIYSFFKSWNSNQTQLDLEAKIEEPL-LKEEAXXXXXXXXXXX 300

Query: 301 XXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360
           XXXXXXXX     GEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD
Sbjct: 301 XXXXXXXXTPPVIGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYAD 360

Query: 361 VSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSL 420
           VSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSL
Sbjct: 361 VSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSL 420

Query: 421 YIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480
           YIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM
Sbjct: 421 YIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDM 480

Query: 481 EATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNKGS 540
           EATP PGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNKGS
Sbjct: 481 EATPNPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNKGS 540

Query: 541 S 542
           S
Sbjct: 541 S 540

BLAST of Carg05163 vs. NCBI nr
Match: XP_022985694.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 891.3 bits (2302), Expect = 1.6e-255
Identity = 457/542 (84.32%), Postives = 483/542 (89.11%), Query Frame = 0

Query: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKD 60
           MGS KLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSD LKSLM+LTQLQLNNLSVAKD
Sbjct: 1   MGSLKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDVLKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120
           VGKAFGLLAGIA DRFPTWVILLIGSLEGL+GYGAQWLVVSQRISPLPYWQMC FLC+GG
Sbjct: 61  VGKAFGLLAGIACDRFPTWVILLIGSLEGLLGYGAQWLVVSQRISPLPYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFS+DPSSFLVML+
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSNDPSSFLVMLS 180

Query: 181 VVPFAVCLFAMFFLREI-PSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVV 240
           VVPFAVCLFAMFFLREI P T  A+ QEES+FFA+FNALAV+VAVYLL FDFIKN G VV
Sbjct: 181 VVPFAVCLFAMFFLREIPPPTTAADTQEESNFFAIFNALAVIVAVYLLCFDFIKNFGTVV 240

Query: 241 SQLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLXXXXXXXXXXXXXXXX 300
           SQLFSVGLLILLGSPL+IP+YS FK+ +SN  Q DLE + EEPL                
Sbjct: 241 SQLFSVGLLILLGSPLIIPMYSFFKNCHSNSAQSDLETQTEEPL-----LKEEATVAAPV 300

Query: 301 XXXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYA 360
                         GEEHTI +A+KT+DFWVLF+SFLCGVGTGLAVMNNMGQIGLALGYA
Sbjct: 301 KEEITEEEQKRPVIGEEHTIIQAVKTIDFWVLFISFLCGVGTGLAVMNNMGQIGLALGYA 360

Query: 361 DVSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGS 420
           DVSMFVSL SIWGFFGRIL+G++SEHFLKK GTPRPLWNAASQILMTIGYILMAMAMPGS
Sbjct: 361 DVSMFVSLISIWGFFGRILSGSLSEHFLKKLGTPRPLWNAASQILMTIGYILMAMAMPGS 420

Query: 421 LYIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYD 480
           LYIGS++VGICYG+RLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYD
Sbjct: 421 LYIGSVIVGICYGIRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYD 480

Query: 481 MEATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNKG 540
           MEATPT GGGNTCIGGHCYRIVFVVMALAC IG  LDIWLA+RTK LYSKLK NK+S KG
Sbjct: 481 MEATPTEGGGNTCIGGHCYRIVFVVMALACGIGLGLDIWLAYRTKGLYSKLKGNKRSKKG 537

Query: 541 SS 542
           SS
Sbjct: 541 SS 537

BLAST of Carg05163 vs. NCBI nr
Match: XP_004147031.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN61919.1 hypothetical protein Csa_2G264630 [Cucumis sativus])

HSP 1 Score: 887.9 bits (2293), Expect = 1.8e-254
Identity = 452/544 (83.09%), Postives = 489/544 (89.89%), Query Frame = 0

Query: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKD 60
           MGS  LPPS TVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLM+LTQLQLNNLSVAKD
Sbjct: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120
           VGKAFGLLAGIASD+FPTWVILLIGS+EGL+GYG QWLVVS+RISPLPYWQMC FLC+GG
Sbjct: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLC ALFS DPSSFL+ML+
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180

Query: 181 VVPFAVCLFAMFFLREIPSTAT---ANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGK 240
           +VP AVCLFAMFFLREIP+  T   A+ Q+ES++F+VFNALAVVVAVYLL FDF+KNSG+
Sbjct: 181 LVPLAVCLFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240

Query: 241 VVSQLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLXXXXXXXXXXXXXX 300
           ++SQL+S+GLLILLGSPL+IPIYS FKSWNS +++LDL    EEPL              
Sbjct: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDL----EEPL---VKEEVVTGAVK 300

Query: 301 XXXXXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG 360
                           GEEHTIFEA++T+DFWVLFVSFLCGVGTGLAVMNNMGQIGLALG
Sbjct: 301 EEAGETAVIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG 360

Query: 361 YADVSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMP 420
           YADVSMFVSLTSIWGFFGRIL+GTISEHFLKK+GTPRPLWNAASQILMT+GY+LMAMAMP
Sbjct: 361 YADVSMFVSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMP 420

Query: 421 GSLYIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL 480
           GSLYIGS++VGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL
Sbjct: 421 GSLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL 480

Query: 481 YDMEATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSN 540
           YDMEATPT GGGNTCIGGHCYRIVF+VMALACVIGFVLDIWLAFRTK LYSKLKANKKS 
Sbjct: 481 YDMEATPTEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKANKKSK 537

Query: 541 KGSS 542
           K +S
Sbjct: 541 KVNS 537

BLAST of Carg05163 vs. TAIR10
Match: AT2G16660.1 (Major facilitator superfamily protein)

HSP 1 Score: 755.7 bits (1950), Expect = 1.9e-218
Identity = 412/541 (76.16%), Postives = 470/541 (86.88%), Query Frame = 0

Query: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKD 60
           MG  +   S++ LKWLGFV+AVWVQ+ISGNNYTFSNYS ALKSLM+L QL+LNNLSVAKD
Sbjct: 1   MGFQRSISSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLNQLELNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120
           VGKAFG+LAG+ASDR PT VILLIG  EGL+GYG QWLVVS+ I P+PYWQMC FLC+GG
Sbjct: 61  VGKAFGILAGLASDRLPTPVILLIGCFEGLLGYGVQWLVVSRTIQPIPYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180
           NSTTWMNTAVLVTCIRNFR+NRGPVSGILKGYVGLSTAIFTDLCTALFS+DP+SFLV+LA
Sbjct: 121 NSTTWMNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCTALFSNDPASFLVLLA 180

Query: 181 VVPFAVCLFAMFFLREI-PSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVV 240
           VVPFAVCL A+FFLREI P+++ A   EE+ +F +FN +AVVVAVYL  +D I     V 
Sbjct: 181 VVPFAVCLTAVFFLREIPPASSAAEENEETRYFTIFNIVAVVVAVYLQSYDIIGIKTGVF 240

Query: 241 SQLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPL--XXXXXXXXXXXXXX 300
           S  F+  LL LL SP+ IP +S  KS N  + Q DLE +I+EPL     XXXXXXXXXXX
Sbjct: 241 SVAFASILLFLLASPIAIPFHSFIKSLNYGE-QDDLEGRIQEPLLRSEIXXXXXXXXXXX 300

Query: 301 XXXXXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG 360
           XXXXXXXXXXXXXX  GE+HTI EA+ TVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG
Sbjct: 301 XXXXXXXXXXXXXXVLGEDHTIMEAVLTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG 360

Query: 361 YADVSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMP 420
           Y +VS+FVS+TSIWGFFGRIL+GT+SE+FLKK+GTPRPLWNAASQILM +GYILMA+A+P
Sbjct: 361 YTNVSIFVSMTSIWGFFGRILSGTLSEYFLKKAGTPRPLWNAASQILMAVGYILMALAVP 420

Query: 421 GSLYIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL 480
            SLYIGSMVVG+CYGVRL+ITVPTASELFGLKYYGLIYNIL+LNLP+GSFLFSGLLAGFL
Sbjct: 421 NSLYIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYNILVLNLPLGSFLFSGLLAGFL 480

Query: 481 YDMEATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSN 539
           YD EATPTPGGGNTC+G HCYR++F+VMALA VIG  LD+ LA+RTK +Y+K+ A+KKS 
Sbjct: 481 YDAEATPTPGGGNTCVGAHCYRLIFIVMALASVIGVGLDLVLAYRTKEIYAKIHASKKSK 540

BLAST of Carg05163 vs. TAIR10
Match: AT4G34950.1 (Major facilitator superfamily protein)

HSP 1 Score: 737.6 bits (1903), Expect = 5.3e-213
Identity = 398/560 (71.07%), Postives = 454/560 (81.07%), Query Frame = 0

Query: 2   GSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKDV 61
           G+S        LKWLGFV+AVWVQ+ISGNNYTFSNYS ALKSLM+LTQL+LN+LSVAKDV
Sbjct: 4   GTSSXXXXXXALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLTQLELNSLSVAKDV 63

Query: 62  GKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGGN 121
           GKAFG+LAG+ASDR  T VILLIGS EGL+GYG QWLVVS+ I P+PYWQMC FLC+GGN
Sbjct: 64  GKAFGILAGLASDRLSTPVILLIGSFEGLLGYGVQWLVVSRTIQPIPYWQMCVFLCMGGN 123

Query: 122 STTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLAV 181
           STTWMNTAVLVTCIRNFR+NRGPVSGILKGYVGLSTAIFTDLC ALFS DP+SFLV+L+V
Sbjct: 124 STTWMNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCNALFSSDPASFLVLLSV 183

Query: 182 VPFAVCLFAMFFLREI-PSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVS 241
           VPFAVCL A+FFLREI PST  A   EES +FAVFN +AVVVAVYL  +D I       S
Sbjct: 184 VPFAVCLTAVFFLREIPPSTTFAEDNEESKYFAVFNIVAVVVAVYLQSYDIIGIKTGAFS 243

Query: 242 QLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPL----------------- 301
             F+  LLILL SP+ +P ++  +S      + D+E +I+EPL                 
Sbjct: 244 IAFASILLILLASPVAVPFHAFIRS--KVHDEQDVEGRIDEPLLRSGSEIEVEETIVGAA 303

Query: 302 -----XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFVSFLCG 361
                         XXXXXXXXXXXXXXXX     GEEHTI EAM TVDFWVLFVSFLCG
Sbjct: 304 AXXDNELPPSLKPLXXXXXXXXXXXXXXXXKRPVLGEEHTIMEAMLTVDFWVLFVSFLCG 363

Query: 362 VGTGLAVMNNMGQIGLALGYADVSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWN 421
           VGTGLAVMNNMGQIGLALGY DVS+FVS+TSIWGFFGRIL+GTISEHF+KK+GTPRPLWN
Sbjct: 364 VGTGLAVMNNMGQIGLALGYTDVSIFVSMTSIWGFFGRILSGTISEHFIKKAGTPRPLWN 423

Query: 422 AASQILMTIGYILMAMAMPGSLYIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNIL 481
           AA+QI+M +GY+LMA+A+PGSLYIGSMVVG+CYGVRL+ITVPTASELFGLKYYGLIYNIL
Sbjct: 424 AAAQIIMAVGYLLMALALPGSLYIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYNIL 483

Query: 482 ILNLPIGSFLFSGLLAGFLYDMEATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIW 539
           ILN+P+GSFLFSGLLAG LYD EATPTPGGGNTC+G HC+RIVF+VMA A +IG  LD+ 
Sbjct: 484 ILNMPLGSFLFSGLLAGLLYDAEATPTPGGGNTCVGAHCFRIVFIVMAFASIIGVGLDLL 543

BLAST of Carg05163 vs. TAIR10
Match: AT5G50630.1 (Major facilitator superfamily protein)

HSP 1 Score: 317.8 bits (813), Expect = 1.3e-86
Identity = 183/526 (34.79%), Postives = 293/526 (55.70%), Query Frame = 0

Query: 14  KWLGFVSAVWVQAISGNNYTF-SNYSDALKSLMSLTQLQLNNLSVAKDVGKAFGLLAGIA 73
           +WL FV A+WVQ+++G  Y F  + S A+K+ +   Q Q+  L VAK++G A G ++G  
Sbjct: 16  RWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGAL 75

Query: 74  SDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGGNSTTWMNTAVLV 133
           S+  P+WV+LL+G+ + L GYG  WLVV+ ++  LP W +   + +G N  T+ NTA LV
Sbjct: 76  SEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLV 135

Query: 134 TCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLAVVPFAVCLFAMF 193
           +CI NF ++RGPV GILKG+ GLS AI T +         SS ++M+A+ P  V L  +F
Sbjct: 136 SCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVILMVALGPPVVVLALLF 195

Query: 194 FLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVSQLFSVG---LLI 253
            +R +  +   N + +   F       VV+AVYLLG   +++   +   + +     L+I
Sbjct: 196 IVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILVI 255

Query: 254 LLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLXXXXXXXXXXXXXXXXXXXXXXXXXX 313
            +  P+++P  S+F S N N T +       +P                           
Sbjct: 256 FMVVPVLVPFSSVFISGN-NVTSV-------KPEEGTSNVDQHEARTLIERSDRPPEKKR 315

Query: 314 XXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMFVSLTS 373
               GE+ T+ +A+   DFW++F+S + GVG+G+ +++N+GQI  +LGY++  +FVSL S
Sbjct: 316 APCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKIFVSLIS 375

Query: 374 IWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSLYIGSMVVGI 433
           I  F GR+  G  SE  ++K   PR L  +  Q +M++G I  A+  PG +Y+ ++V+G+
Sbjct: 376 ISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGM 435

Query: 434 CYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATPTPGGG 493
            YG   +I   + S++FGLK +G +YN  I  LPIGSF+FSG++A  +YD  A    G  
Sbjct: 436 GYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQAGPT 495

Query: 494 N-----TCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKL 531
                  C G  CY +   +M++ C++  VL + + +RT+  Y +L
Sbjct: 496 TETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFYLRL 533

BLAST of Carg05163 vs. TAIR10
Match: AT5G50520.1 (Major facilitator superfamily protein)

HSP 1 Score: 317.8 bits (813), Expect = 1.3e-86
Identity = 183/526 (34.79%), Postives = 293/526 (55.70%), Query Frame = 0

Query: 14  KWLGFVSAVWVQAISGNNYTF-SNYSDALKSLMSLTQLQLNNLSVAKDVGKAFGLLAGIA 73
           +WL FV A+WVQ+++G  Y F  + S A+K+ +   Q Q+  L VAK++G A G ++G  
Sbjct: 16  RWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGAL 75

Query: 74  SDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGGNSTTWMNTAVLV 133
           S+  P+WV+LL+G+ + L GYG  WLVV+ ++  LP W +   + +G N  T+ NTA LV
Sbjct: 76  SEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLV 135

Query: 134 TCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLAVVPFAVCLFAMF 193
           +CI NF ++RGPV GILKG+ GLS AI T +         SS ++M+A+ P  V L  +F
Sbjct: 136 SCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVILMVALGPPVVVLALLF 195

Query: 194 FLREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVSQLFSVG---LLI 253
            +R +  +   N + +   F       VV+AVYLLG   +++   +   + +     L+I
Sbjct: 196 IVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILVI 255

Query: 254 LLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLXXXXXXXXXXXXXXXXXXXXXXXXXX 313
            +  P+++P  S+F S N N T +       +P                           
Sbjct: 256 FMVVPVLVPFSSVFISGN-NVTSV-------KPEEGTSNVDQHEARTLIERSDRPPEKKR 315

Query: 314 XXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMFVSLTS 373
               GE+ T+ +A+   DFW++F+S + GVG+G+ +++N+GQI  +LGY++  +FVSL S
Sbjct: 316 APCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKIFVSLIS 375

Query: 374 IWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSLYIGSMVVGI 433
           I  F GR+  G  SE  ++K   PR L  +  Q +M++G I  A+  PG +Y+ ++V+G+
Sbjct: 376 ISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGM 435

Query: 434 CYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATPTPGGG 493
            YG   +I   + S++FGLK +G +YN  I  LPIGSF+FSG++A  +YD  A    G  
Sbjct: 436 GYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQAGPT 495

Query: 494 N-----TCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKL 531
                  C G  CY +   +M++ C++  VL + + +RT+  Y +L
Sbjct: 496 TETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFYLRL 533

BLAST of Carg05163 vs. TAIR10
Match: AT3G01930.2 (Major facilitator superfamily protein)

HSP 1 Score: 314.7 bits (805), Expect = 1.1e-85
Identity = 191/564 (33.87%), Postives = 290/564 (51.42%), Query Frame = 0

Query: 14  KWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKDVGKAFGLLAGIAS 73
           +WL FV+A+W+Q+ +G  Y F + S  +KS ++  Q QL+ L VAKD+G + G LAG  S
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVGFLAGTLS 75

Query: 74  DRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGGNSTTWMNTAVLVT 133
           +  P W  LL+GS++ L+GYG  WL+V+ R   LP W MC  + +G N  T+ NTA LV+
Sbjct: 76  EILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYFNTAALVS 135

Query: 134 CIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLAVVPFAVCLFAMFF 193
            ++NF K+RGPV GILKG+ GL  AI + + T + S D +S + M+AV P  V +  MFF
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPSVVVVPLMFF 195

Query: 194 LREIPSTATANAQEESSFFAVFNALAVVVAVYLLGF----DFIKNSGKVVSQLFSVGLLI 253
           +R +       + + +SF  ++ A+ +++A YL+      DFI  S  ++          
Sbjct: 196 IRPVGGHRQIRSSDATSFTVIY-AVCILLAAYLMAVMLVEDFIDLSHSIIXXXXXXXXXX 255

Query: 254 LLGSPLVIPIYSLFKSWNSNQTQLD--------------------------LEAKIEEP- 313
                      S F +       L+                           E + E+P 
Sbjct: 256 XXXXXXXXXXXSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSEVEDEKPK 315

Query: 314 ---------LXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFV 373
                                                   GE+ T+ +A+   DFW++F 
Sbjct: 316 EVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKADFWLIFF 375

Query: 374 SFLCGVGTGLAVMNNMGQIGLALGYADVSMFVSLTSIWGFFGRILAGTISEHFLKKSGTP 433
           S L G G+GL V++N+GQ+  +LGY +  +FVS+ SIW F GRI  G  SE  ++    P
Sbjct: 376 SLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWNFLGRIGGGYFSELIVRDYAYP 435

Query: 434 RPLWNAASQILMTIGYILMAMAMPGSLYIGSMVVGICYGVRLSITVPTASELFGLKYYGL 493
           RP+  A +Q++M++G+I  A   PG+++IG++++G+ YG   +I   TASELFGLK +G 
Sbjct: 436 RPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASELFGLKKFGA 495

Query: 494 IYNILILNLPIGSFLFSGLLAGFLYDMEATP-------TPGGGNTCIGGHCYRIVFVVMA 531
           +YN L L  P GS +FSGL+A  +YD EA          P     C G  CY +  ++M+
Sbjct: 496 LYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDVLRCRGSICYFLTSLIMS 555

BLAST of Carg05163 vs. Swiss-Prot
Match: sp|F4I9E1|NFD4_ARATH (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 4.1e-61
Identity = 167/550 (30.36%), Postives = 275/550 (50.00%), Query Frame = 0

Query: 6   LPPSTTVL----KWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKDV 65
           LPP  T+     KW   V+A+W+QA +G N+ FS YS  LKS++ ++Q++LN L+VA D+
Sbjct: 31  LPPLRTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDL 90

Query: 66  GKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLC-LGG 125
           GKAFG  +GIA   FP  V+L   +  G +GYG QWLV++  I+ LPY  + F  C L G
Sbjct: 91  GKAFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIIT-LPY-SLVFLCCLLAG 150

Query: 126 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 185
            S  W NTA  + CIR+F  NR     +   + G+S A+++    A+     + +L++ +
Sbjct: 151 LSICWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNS 210

Query: 186 VVPFAVCLFAMFFLREIPSTATA----NAQEESSFFAVFNALAVVVAVYLLGFDFIKNSG 245
           +VP  V   A++ +   PS  T     + + +S  F + N LAV+ + +LL    + +S 
Sbjct: 211 LVPLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLL----LSSSS 270

Query: 246 KVVSQLFSVGLLILLGSPLVIPIY----SLFKSWNSNQTQLDLEAKIEEPLXXXXXXXXX 305
              ++L  +G ++LL  PL  P+       F    + +   +    +   +         
Sbjct: 271 TSSARLNFIGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTS 330

Query: 306 XXXXXXXXXXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQI 365
                                G+EH+    +  ++FW+ ++++ CG   GL   NN+GQI
Sbjct: 331 VSSKTGYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQI 390

Query: 366 GLALGYADVSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILM 425
             +LG    ++ V++ S + FFGR+L+    +   K+    R  W A + +   I + L+
Sbjct: 391 AQSLGQNSTTL-VTIYSSFSFFGRLLSAA-PDFMHKRFRLTRTGWFAIALLPTPIAFFLL 450

Query: 426 AM--AMPGSLYIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFS 485
           A+  +   +L   + ++G+  G   +  V   S+LFG    G+ +NILI N+PIGS L+ 
Sbjct: 451 AVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLY- 510

Query: 486 GLLAGFLYDMEA----TPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVL 537
           G +A  +Y+  A    TP       CIG  CY   FV      ++G V  + L  RTK +
Sbjct: 511 GYIAASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPV 570

BLAST of Carg05163 vs. TrEMBL
Match: tr|A0A0A0LLL4|A0A0A0LLL4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G264630 PE=4 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 1.2e-254
Identity = 452/544 (83.09%), Postives = 489/544 (89.89%), Query Frame = 0

Query: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKD 60
           MGS  LPPS TVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLM+LTQLQLNNLSVAKD
Sbjct: 1   MGSVNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120
           VGKAFGLLAGIASD+FPTWVILLIGS+EGL+GYG QWLVVS+RISPLPYWQMC FLC+GG
Sbjct: 61  VGKAFGLLAGIASDKFPTWVILLIGSVEGLVGYGTQWLVVSRRISPLPYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLC ALFS DPSSFL+ML+
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180

Query: 181 VVPFAVCLFAMFFLREIPSTAT---ANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGK 240
           +VP AVCLFAMFFLREIP+  T   A+ Q+ES++F+VFNALAVVVAVYLL FDF+KNSG+
Sbjct: 181 LVPLAVCLFAMFFLREIPTQTTITAADTQQESNYFSVFNALAVVVAVYLLCFDFVKNSGR 240

Query: 241 VVSQLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLXXXXXXXXXXXXXX 300
           ++SQL+S+GLLILLGSPL+IPIYS FKSWNS +++LDL    EEPL              
Sbjct: 241 LISQLYSIGLLILLGSPLIIPIYSFFKSWNSIRSRLDL----EEPL---VKEEVVTGAVK 300

Query: 301 XXXXXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG 360
                           GEEHTIFEA++T+DFWVLFVSFLCGVGTGLAVMNNMGQIGLALG
Sbjct: 301 EEAGETAVIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG 360

Query: 361 YADVSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMP 420
           YADVSMFVSLTSIWGFFGRIL+GTISEHFLKK+GTPRPLWNAASQILMT+GY+LMAMAMP
Sbjct: 361 YADVSMFVSLTSIWGFFGRILSGTISEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMP 420

Query: 421 GSLYIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL 480
           GSLYIGS++VGICYGVRLS+TVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL
Sbjct: 421 GSLYIGSVIVGICYGVRLSVTVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL 480

Query: 481 YDMEATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSN 540
           YDMEATPT GGGNTCIGGHCYRIVF+VMALACVIGFVLDIWLAFRTK LYSKLKANKKS 
Sbjct: 481 YDMEATPTEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKANKKSK 537

Query: 541 KGSS 542
           K +S
Sbjct: 541 KVNS 537

BLAST of Carg05163 vs. TrEMBL
Match: tr|A0A1S3C607|A0A1S3C607_CUCME (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497317 PE=4 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 3.2e-252
Identity = 451/542 (83.21%), Postives = 487/542 (89.85%), Query Frame = 0

Query: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKD 60
           MGS  LPPS TVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLM+LTQLQLNNLSVAKD
Sbjct: 1   MGSLNLPPSMTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMNLTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120
           VGKAFGLLAG+ASDRFPTWVILLIGS+EGL+GYGAQWLVVS+RISPL YWQMC FLC+GG
Sbjct: 61  VGKAFGLLAGMASDRFPTWVILLIGSVEGLVGYGAQWLVVSRRISPLSYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLC ALFS DPSSFL+ML+
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCFALFSSDPSSFLLMLS 180

Query: 181 VVPFAVCLFAMFFLREIPSTAT---ANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGK 240
           +VPFAVCLFAMFFLREIP+  T   A+ Q+ES++F+VFN LAVVVAVYLL FDFIKNSG+
Sbjct: 181 LVPFAVCLFAMFFLREIPTQITITAADTQQESNYFSVFNVLAVVVAVYLLCFDFIKNSGR 240

Query: 241 VVSQLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLXXXXXXXXXXXXXX 300
           ++SQL+S+GLLILLGSPL+IPIYS FKSWNS +++LDL    EEPL              
Sbjct: 241 LISQLYSMGLLILLGSPLIIPIYSFFKSWNSIRSRLDL----EEPL---VKEEAVTGAVK 300

Query: 301 XXXXXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG 360
                           GEEHTIFEA++T+DFWVLFVSFLCGVGTGLAVMNNMGQIGLALG
Sbjct: 301 EETGEIAMIEQRAPVIGEEHTIFEAVRTIDFWVLFVSFLCGVGTGLAVMNNMGQIGLALG 360

Query: 361 YADVSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMP 420
           YADVSMFVSLTSIWGFFGRIL+GT+SEHFLKK+GTPRPLWNAASQILMT+GY+LMAMAMP
Sbjct: 361 YADVSMFVSLTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMTVGYVLMAMAMP 420

Query: 421 GSLYIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL 480
           GSLYIGS++VGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL
Sbjct: 421 GSLYIGSVIVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFL 480

Query: 481 YDMEATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLK-ANKKS 539
           YDMEATPT GGGNTCIGGHCYRIVF+VMALACVIGFVLDIWLAFRTK LYSKLK ANK+S
Sbjct: 481 YDMEATPTEGGGNTCIGGHCYRIVFLVMALACVIGFVLDIWLAFRTKELYSKLKAANKRS 535

BLAST of Carg05163 vs. TrEMBL
Match: tr|A0A087HD71|A0A087HD71_ARAAL (Uncharacterized protein OS=Arabis alpina OX=50452 GN=AALP_AA3G326700 PE=4 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 4.2e-220
Identity = 394/540 (72.96%), Postives = 449/540 (83.15%), Query Frame = 0

Query: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKD 60
           MG  + P +++ LKWLGFV+AVWVQAISGNNYTFSNYS ALKSLM+L QL+LNNLSVAKD
Sbjct: 1   MGFQRSPSTSSALKWLGFVTAVWVQAISGNNYTFSNYSGALKSLMNLNQLELNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120
           VGKAFG+LAG+ASDR PT VILLIG  EGL+GYG QWLVVS+ I+PLPYWQMC FLC+GG
Sbjct: 61  VGKAFGILAGLASDRLPTPVILLIGCFEGLLGYGVQWLVVSRTINPLPYWQMCVFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180
           NSTTWMNTAVLVTCIRNFR+NRGPVSGILKGYVGLSTAIFTDLCTALFS+DP+SFLV+LA
Sbjct: 121 NSTTWMNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCTALFSNDPASFLVLLA 180

Query: 181 VVPFAVCLFAMFFLREIPSTATANA-QEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVV 240
           VVPFAVCL A+FFLREIP +++A+   EE+ +FAVFN +AVVVAVYL  +D I     V 
Sbjct: 181 VVPFAVCLAAVFFLREIPPSSSADEDNEETRYFAVFNIVAVVVAVYLQSYDIIGIKTGVF 240

Query: 241 SQLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEP-LXXXXXXXXXXXXXXX 300
           S  F+  LL LL SP+ IP +S  KS N N  Q DLE +I+EP L               
Sbjct: 241 SVAFASILLFLLASPIAIPFHSFIKSLNRNGDQEDLEGRIQEPLLRSENEAEKEVIGVAA 300

Query: 301 XXXXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGY 360
                          GE+HTI EA+ TVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGY
Sbjct: 301 EEVERPVTEKKRPVLGEDHTIMEALLTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGY 360

Query: 361 ADVSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPG 420
            DVS+FVS+TSIWGFFGRIL+GT+SEHFLKK+GTPRPLWNAASQILM +GYILMA+A+P 
Sbjct: 361 TDVSIFVSMTSIWGFFGRILSGTLSEHFLKKAGTPRPLWNAASQILMAVGYILMALAVPN 420

Query: 421 SLYIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLY 480
           SLYIGSMVVG+CYGVRL+ITVPTASELFGLKYYGLIYNIL+LNLP+GSFLFSGLLAGFLY
Sbjct: 421 SLYIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYNILVLNLPLGSFLFSGLLAGFLY 480

Query: 481 DMEATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNK 539
           D EATPTPGGGNTC+G HCYR++F+VMALA VIG  LD+ LA+RTK +Y+K+ A+KKS K
Sbjct: 481 DAEATPTPGGGNTCVGAHCYRLIFIVMALASVIGVGLDLILAYRTKEIYAKIHASKKSKK 540

BLAST of Carg05163 vs. TrEMBL
Match: tr|A0A1U8AGP0|A0A1U8AGP0_NELNU (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Nelumbo nucifera OX=4432 GN=LOC104604227 PE=4 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 5.4e-220
Identity = 385/539 (71.43%), Postives = 448/539 (83.12%), Query Frame = 0

Query: 1   MGSSKLPPSTTVLKWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKD 60
           MG + L PS++  KWLGFV+AVW+QAISGNNYTFSNYSDALKSLM+LTQLQLNNLSVAKD
Sbjct: 1   MGETLLSPSSSAGKWLGFVTAVWIQAISGNNYTFSNYSDALKSLMALTQLQLNNLSVAKD 60

Query: 61  VGKAFGLLAGIASDRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGG 120
           VGKAFGLLAG+ASDR PT V+LLIGS+EG IGYG QWLV+SQRI PLPYWQMC FLC+GG
Sbjct: 61  VGKAFGLLAGLASDRLPTSVLLLIGSVEGFIGYGVQWLVISQRIRPLPYWQMCIFLCMGG 120

Query: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLA 180
           NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFS DPS+F++ML+
Sbjct: 121 NSTTWMNTAVLVTCIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSADPSTFVLMLS 180

Query: 181 VVPFAVCLFAMFFLREI-PSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVV 240
           ++P  VC   + FLRE+ PS+ +A   EES +F +FN +AV++AVYLL FD   N G+++
Sbjct: 181 IIPALVCFTGILFLREVPPSSGSAQENEESHYFNIFNVIAVIIAVYLLAFDVTGNHGRLL 240

Query: 241 SQLFSVGLLILLGSPLVIPIYSLFKSWNSNQTQLDLEAKIEEPLXXXXXXXXXXXXXXXX 300
           S  FS+ LLILL +PL IP+YS+F++W  N    DLE +I+EPL                
Sbjct: 241 SVAFSIVLLILLVAPLAIPLYSVFQNWGLNSRSGDLEHQIKEPLLVQGTAPITQQQKPQE 300

Query: 301 XXXXXXXXXXXXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYA 360
                         GEEHTI EAMKT+DFW+LFVSFLCGVGTGLAVMNNMGQ+GLALGYA
Sbjct: 301 KVGEVEMRPSPPVIGEEHTIVEAMKTLDFWILFVSFLCGVGTGLAVMNNMGQMGLALGYA 360

Query: 361 DVSMFVSLTSIWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGS 420
           DVS+FVSL SIWGFFGRI++GT SE+F+KK+ TPRPLWNA SQI+M +GY+L+A+A+PGS
Sbjct: 361 DVSIFVSLISIWGFFGRIISGTASEYFIKKAATPRPLWNAVSQIVMAVGYVLLALALPGS 420

Query: 421 LYIGSMVVGICYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYD 480
           LYIGS++VG+CYGVRL+ITV TASELFGLKYYGLIYNILILNLP+GSFLFSGLLAGFLYD
Sbjct: 421 LYIGSIIVGLCYGVRLAITVATASELFGLKYYGLIYNILILNLPLGSFLFSGLLAGFLYD 480

Query: 481 MEATPTPGGGNTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNK 539
            EAT    GGNTCIG HCYR+VFVVMALACV+GF LD+ LAFRTK +YSK+ A+KKS K
Sbjct: 481 AEATSIAEGGNTCIGAHCYRLVFVVMALACVLGFGLDVVLAFRTKNVYSKIYASKKSKK 539

BLAST of Carg05163 vs. TrEMBL
Match: tr|A0A1S3U810|A0A1S3U810_VIGRR (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Vigna radiata var. radiata OX=3916 GN=LOC106762625 PE=4 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 1.8e-218
Identity = 383/531 (72.13%), Postives = 443/531 (83.43%), Query Frame = 0

Query: 14  KWLGFVSAVWVQAISGNNYTFSNYSDALKSLMSLTQLQLNNLSVAKDVGKAFGLLAGIAS 73
           KW+G V+AVW+QAISGNNYTFSNYSDALKSLM LTQ+QLNNLSVAKDVGKAFGLLAG+AS
Sbjct: 11  KWIGLVAAVWIQAISGNNYTFSNYSDALKSLMHLTQVQLNNLSVAKDVGKAFGLLAGLAS 70

Query: 74  DRFPTWVILLIGSLEGLIGYGAQWLVVSQRISPLPYWQMCFFLCLGGNSTTWMNTAVLVT 133
           D+FPTW+ILLIGS+EGL+GYG QWLVVSQRI PLPY+QMC FLCLGGNSTTWMNTAVLVT
Sbjct: 71  DKFPTWIILLIGSVEGLVGYGVQWLVVSQRIQPLPYYQMCIFLCLGGNSTTWMNTAVLVT 130

Query: 134 CIRNFRKNRGPVSGILKGYVGLSTAIFTDLCTALFSDDPSSFLVMLAVVPFAVCLFAMFF 193
           CIRNFR+NRGPVSGILKGYVGLSTAIFTDLC+ALF+DDP+SFL+MLA++P  VCL  +FF
Sbjct: 131 CIRNFRRNRGPVSGILKGYVGLSTAIFTDLCSALFADDPASFLIMLALIPLVVCLSGIFF 190

Query: 194 LREIPSTATANAQEESSFFAVFNALAVVVAVYLLGFDFIKNSGKVVSQLFSVGLLILLGS 253
           LREIP  A  +  EES++F VFNA+AV VAVYLL FDF+ N    VS+ FSVGLL+LL +
Sbjct: 191 LREIPPAAVQD-DEESTYFGVFNAVAVAVAVYLLAFDFVPNPSAFVSRAFSVGLLVLLAA 250

Query: 254 PLVIPIYSLFKSWNSNQTQLDLEAKIEEPL----XXXXXXXXXXXXXXXXXXXXXXXXXX 313
           PL IP++S  K+   ++ + D E + EEPL                              
Sbjct: 251 PLGIPVHSYLKARGLDKFRPDFEGRGEEPLLRGNDNGNECEVQRGNVLAEDAEAVAVGKS 310

Query: 314 XXXXGEEHTIFEAMKTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYADVSMFVSLTS 373
               GEEHT++E ++TVDFW+LFVSFLCGVGTGLAVMNNMGQIGLALGY+DVS+FVSLTS
Sbjct: 311 KPAVGEEHTVWEVLRTVDFWILFVSFLCGVGTGLAVMNNMGQIGLALGYSDVSLFVSLTS 370

Query: 374 IWGFFGRILAGTISEHFLKKSGTPRPLWNAASQILMTIGYILMAMAMPGSLYIGSMVVGI 433
           IWGFFGRI++G++SEHF+K+ GTPRPLWNA SQILM +GYIL+AMAMPGSLYIGS+VVGI
Sbjct: 371 IWGFFGRIVSGSVSEHFIKRGGTPRPLWNAGSQILMGVGYILLAMAMPGSLYIGSIVVGI 430

Query: 434 CYGVRLSITVPTASELFGLKYYGLIYNILILNLPIGSFLFSGLLAGFLYDMEATPTPGGG 493
           CYGVRL+ITVPTASELFGLKYYGLIYNILILNLP+GSFLFSGLLAG LYDMEAT T GGG
Sbjct: 431 CYGVRLAITVPTASELFGLKYYGLIYNILILNLPLGSFLFSGLLAGILYDMEATTTTGGG 490

Query: 494 NTCIGGHCYRIVFVVMALACVIGFVLDIWLAFRTKVLYSKLKANKKSNKGS 541
           NTCIG HCYR+VF+VM+ AC+IGF LD+ L+FRTK+LY  +   K SNK +
Sbjct: 491 NTCIGPHCYRLVFIVMSAACLIGFFLDLLLSFRTKMLYKTIYFTKISNKNA 540

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023532459.13.3e-28599.82protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo][more]
XP_022948188.12.1e-28498.89protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata][more]
XP_022970978.18.9e-28397.23protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima][more]
XP_022985694.11.6e-25584.32protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima][more]
XP_004147031.11.8e-25483.09PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN61919.1 hypo... [more]
Match NameE-valueIdentityDescription
AT2G16660.11.9e-21876.16Major facilitator superfamily protein[more]
AT4G34950.15.3e-21371.07Major facilitator superfamily protein[more]
AT5G50630.11.3e-8634.79Major facilitator superfamily protein[more]
AT5G50520.11.3e-8634.79Major facilitator superfamily protein[more]
AT3G01930.21.1e-8533.87Major facilitator superfamily protein[more]
Match NameE-valueIdentityDescription
sp|F4I9E1|NFD4_ARATH4.1e-6130.36Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LLL4|A0A0A0LLL4_CUCSA1.2e-25483.09Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G264630 PE=4 SV=1[more]
tr|A0A1S3C607|A0A1S3C607_CUCME3.2e-25283.21protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497317 PE=4 ... [more]
tr|A0A087HD71|A0A087HD71_ARAAL4.2e-22072.96Uncharacterized protein OS=Arabis alpina OX=50452 GN=AALP_AA3G326700 PE=4 SV=1[more]
tr|A0A1U8AGP0|A0A1U8AGP0_NELNU5.4e-22071.43protein NUCLEAR FUSION DEFECTIVE 4-like OS=Nelumbo nucifera OX=4432 GN=LOC104604... [more]
tr|A0A1S3U810|A0A1S3U810_VIGRR1.8e-21872.13protein NUCLEAR FUSION DEFECTIVE 4-like OS=Vigna radiata var. radiata OX=3916 GN... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR010658Nodulin-like
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05163-RACarg05163-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 317..526
e-value: 4.9E-11
score: 44.2
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 12..208
e-value: 3.0E-8
score: 35.2
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 9..539
NoneNo IPR availablePANTHERPTHR21576:SF17MAJOR FACILITATOR FAMILY PROTEINcoord: 9..539
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 14..259
e-value: 1.0E-93
score: 313.3
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 310..524
coord: 59..209

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg05163Cla020293Watermelon (97103) v1carwmB0808
Carg05163Cla015835Watermelon (97103) v1carwmB0813
Carg05163Csa2G264630Cucumber (Chinese Long) v2carcuB0818
Carg05163Csa7G011800Cucumber (Chinese Long) v2carcuB0828
Carg05163MELO3C013366Melon (DHL92) v3.5.1carmeB0801
Carg05163MELO3C020805Melon (DHL92) v3.5.1carmeB0800
Carg05163ClCG02G004200Watermelon (Charleston Gray)carwcgB0784
Carg05163ClCG02G011780Watermelon (Charleston Gray)carwcgB0780
Carg05163CSPI07G00880Wild cucumber (PI 183967)carcpiB0848
Carg05163CSPI02G13390Wild cucumber (PI 183967)carcpiB0838
Carg05163Cucsa.234390Cucumber (Gy14) v1carcgyB0922
Carg05163Cucsa.370610Cucumber (Gy14) v1carcgyB0925
Carg05163CmaCh20G007610Cucurbita maxima (Rimu)carcmaB1122
Carg05163CmaCh02G002430Cucurbita maxima (Rimu)carcmaB1124
Carg05163CmoCh20G007580Cucurbita moschata (Rifu)carcmoB1093
Carg05163CmoCh02G002450Cucurbita moschata (Rifu)carcmoB1094
Carg05163Lsi10G004820Bottle gourd (USVL1VR-Ls)carlsiB738
Carg05163Lsi11G011940Bottle gourd (USVL1VR-Ls)carlsiB740
Carg05163Cp4.1LG16g03080Cucurbita pepo (Zucchini)carcpeB1131
Carg05163Cp4.1LG05g13740Cucurbita pepo (Zucchini)carcpeB1139
Carg05163CsGy7G000720Cucumber (Gy14) v2carcgybB0801
Carg05163CsGy2G013280Cucumber (Gy14) v2carcgybB0792
Carg05163MELO3C020805.2Melon (DHL92) v3.6.1carmedB0812
Carg05163MELO3C013366.2Melon (DHL92) v3.6.1carmedB0813
Carg05163CsaV3_7G000910Cucumber (Chinese Long) v3carcucB0851
Carg05163CsaV3_2G015910Cucumber (Chinese Long) v3carcucB0842
Carg05163Cla97C02G037390Watermelon (97103) v2carwmbB0842
Carg05163Cla97C02G031010Watermelon (97103) v2carwmbB0847
Carg05163Bhi05G001484Wax gourdcarwgoB0940
Carg05163Bhi10G001475Wax gourdcarwgoB0943
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg05163Carg16183Silver-seed gourdcarcarB373
The following block(s) are covering this gene:
GeneOrganismBlock
Carg05163Cucurbita maxima (Rimu)carcmaB1120
Carg05163Cucurbita moschata (Rifu)carcmoB1091
Carg05163Watermelon (Charleston Gray)carwcgB0782
Carg05163Watermelon (97103) v1carwmB0809