Carg04937 (gene) Silver-seed gourd

Overview
NameCarg04937
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionrRNA N-glycosidase
LocationCucurbita_argyrosperma_scaffold_060 : 337303 .. 338181 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAAACTCATAGCAATCACCTCTTTCTTACTCGCCATCTCTCTTATCTCTCCCACTCTCAAGGCTGACATAGCCTTCGATTTGTCCACAGCTACCAAAGCATCCTATTCAGCTTTCATAACCCAGCTCAGGAATGCTCTTCCAACCAAAGCAACAGTGTGCAACATACCCCTGCTGCCTTCCACCGCAACGGGCTTAGGCTGGTTCACATTCTTCACCATCACCAATTATAACTACCAAACCGTCGAGGTGGCTGTGAACGTATCCAACGTCTACATCGTAGCGTATCGTGCCGATGCAAACTCCTACTTTTTCGAAGACACTCCAGCTCAAGCCAAACAACTCTTATTTGCAGGTACTCAAAAGGTAACACTTCCTTTTACGGGTAACTATGATAAGCTCCAAAGCATTGTAGGCAAATATAGAGATATGGTTGAGCTTGGAATCCCCGCCTTGAGCACTGCAGTTACCAACATGGTTTACTACAATAAACAACCGACTGCGGCGGCGCTTCTTGTGCTCATTCAGTCCACTGCTGAAGCTGCGAGGTTTAAATATATTGAGCAACAGGTTGCTCAGTATATTAGCGGTAAGTTTTATCCAAACCAAGCAGTGATAAGCTTAGAGAACAACTGGGGAGCTCTCTCCAAGCAAATCCAGATAGCAAATAGTACTGGAAGTGGACAATTCCAGAGCCCCGTTGAGCTTATAAGTCCTAATGGCACACGATTTAGGGTAACCAGTACATCATCTGGGGTTGTTCAAGGCAATATCAAGCTCCTGCTGTACTACAAACTGAGCGTTGCTGATGACAACGACAGCCCGACAAGAATGCCTGACAATATTGTTGAATATGGAGCTCATGATTCTATGTAG

mRNA sequence

ATGAACAAACTCATAGCAATCACCTCTTTCTTACTCGCCATCTCTCTTATCTCTCCCACTCTCAAGGCTGACATAGCCTTCGATTTGTCCACAGCTACCAAAGCATCCTATTCAGCTTTCATAACCCAGCTCAGGAATGCTCTTCCAACCAAAGCAACAGTGTGCAACATACCCCTGCTGCCTTCCACCGCAACGGGCTTAGGCTGGTTCACATTCTTCACCATCACCAATTATAACTACCAAACCGTCGAGGTGGCTGTGAACGTATCCAACGTCTACATCGTAGCGTATCGTGCCGATGCAAACTCCTACTTTTTCGAAGACACTCCAGCTCAAGCCAAACAACTCTTATTTGCAGGTACTCAAAAGGTAACACTTCCTTTTACGGGTAACTATGATAAGCTCCAAAGCATTGTAGGCAAATATAGAGATATGGTTGAGCTTGGAATCCCCGCCTTGAGCACTGCAGTTACCAACATGGTTTACTACAATAAACAACCGACTGCGGCGGCGCTTCTTGTGCTCATTCAGTCCACTGCTGAAGCTGCGAGGTTTAAATATATTGAGCAACAGGTTGCTCAGTATATTAGCGGTAAGTTTTATCCAAACCAAGCAGTGATAAGCTTAGAGAACAACTGGGGAGCTCTCTCCAAGCAAATCCAGATAGCAAATAGTACTGGAAGTGGACAATTCCAGAGCCCCGTTGAGCTTATAAGTCCTAATGGCACACGATTTAGGGTAACCAGTACATCATCTGGGGTTGTTCAAGGCAATATCAAGCTCCTGCTGTACTACAAACTGAGCGTTGCTGATGACAACGACAGCCCGACAAGAATGCCTGACAATATTGTTGAATATGGAGCTCATGATTCTATGTAG

Coding sequence (CDS)

ATGAACAAACTCATAGCAATCACCTCTTTCTTACTCGCCATCTCTCTTATCTCTCCCACTCTCAAGGCTGACATAGCCTTCGATTTGTCCACAGCTACCAAAGCATCCTATTCAGCTTTCATAACCCAGCTCAGGAATGCTCTTCCAACCAAAGCAACAGTGTGCAACATACCCCTGCTGCCTTCCACCGCAACGGGCTTAGGCTGGTTCACATTCTTCACCATCACCAATTATAACTACCAAACCGTCGAGGTGGCTGTGAACGTATCCAACGTCTACATCGTAGCGTATCGTGCCGATGCAAACTCCTACTTTTTCGAAGACACTCCAGCTCAAGCCAAACAACTCTTATTTGCAGGTACTCAAAAGGTAACACTTCCTTTTACGGGTAACTATGATAAGCTCCAAAGCATTGTAGGCAAATATAGAGATATGGTTGAGCTTGGAATCCCCGCCTTGAGCACTGCAGTTACCAACATGGTTTACTACAATAAACAACCGACTGCGGCGGCGCTTCTTGTGCTCATTCAGTCCACTGCTGAAGCTGCGAGGTTTAAATATATTGAGCAACAGGTTGCTCAGTATATTAGCGGTAAGTTTTATCCAAACCAAGCAGTGATAAGCTTAGAGAACAACTGGGGAGCTCTCTCCAAGCAAATCCAGATAGCAAATAGTACTGGAAGTGGACAATTCCAGAGCCCCGTTGAGCTTATAAGTCCTAATGGCACACGATTTAGGGTAACCAGTACATCATCTGGGGTTGTTCAAGGCAATATCAAGCTCCTGCTGTACTACAAACTGAGCGTTGCTGATGACAACGACAGCCCGACAAGAATGCCTGACAATATTGTTGAATATGGAGCTCATGATTCTATGTAG

Protein sequence

MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLLPSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAGTQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTAEAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISPNGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM
Homology
[Close]
BLAST of Carg04937 vs. NCBI nr
Match: XP_022960460.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita moschata])

HSP 1 Score: 530.0 bits (1364), Expect = 5.0e-147
Identity = 275/292 (94.18%), Postives = 283/292 (96.92%), Query Frame = 0 

Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           MNKLIAITSFLLAISLISPTLKADIA DLSTATKA+YSAFITQLRNALPTKATVCNIPLL
Sbjct: 1   MNKLIAITSFLLAISLISPTLKADIALDLSTATKATYSAFITQLRNALPTKATVCNIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
           PSTATGLG FTFFTITNYNY+TVEVAVNVSNVYIVAYRA+A SYFFEDT AQA+QLLF  
Sbjct: 61  PSTATGLGSFTFFTITNYNYETVEVAVNVSNVYIVAYRANAKSYFFEDTSAQARQLLFQN 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           TQK+TLPFTGNYD+LQ +VGKYRD +ELGIPALSTAVTNMVYYNKQ TAAALLVLIQSTA
Sbjct: 121 TQKITLPFTGNYDRLQGVVGKYRDSIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSTA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP
Sbjct: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 293
           NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM
Sbjct: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 292

[Close]
BLAST of Carg04937 vs. NCBI nr
Match: XP_023513430.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521.9 bits (1343), Expect = 1.4e-144
Identity = 273/292 (93.49%), Postives = 280/292 (95.89%), Query Frame = 0 

Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           MNKLIAI+SFLLAISLISPTLKADIA DLSTATKASYSAFIT +RNALPTKATVCNIPLL
Sbjct: 1   MNKLIAISSFLLAISLISPTLKADIALDLSTATKASYSAFITHIRNALPTKATVCNIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
           PST TGL  FTFFTITNYNYQTVEVAVNV+NVYIVAYRA A+SYFFEDTPAQAKQLLFAG
Sbjct: 61  PSTPTGLASFTFFTITNYNYQTVEVAVNVTNVYIVAYRAGASSYFFEDTPAQAKQLLFAG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQ TAAALLVLIQSTA
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQLTAAALLVLIQSTA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIEQQVAQ+IS KFYPNQAVISLENNWGALSKQIQIANSTG GQFQSPVELI P
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELIGP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 293
            GTRF VT+TS+GVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM
Sbjct: 241 TGTRFSVTNTSAGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 292

[Close]
BLAST of Carg04937 vs. NCBI nr
Match: XP_023005055.1 (type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima])

HSP 1 Score: 521.2 bits (1341), Expect = 2.3e-144
Identity = 268/292 (91.78%), Postives = 281/292 (96.23%), Query Frame = 0 

Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           MNKLIAITSFL+AISLISPTLKADI FDLSTATKASYSAFITQLRNALPT ATVCNIPLL
Sbjct: 1   MNKLIAITSFLVAISLISPTLKADITFDLSTATKASYSAFITQLRNALPTTATVCNIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
           PSTA+GLGWFTFFTITNYNYQT+ VAVNV+NVYIVAYRADA+SYFFEDTPA+AKQLLF  
Sbjct: 61  PSTASGLGWFTFFTITNYNYQTITVAVNVTNVYIVAYRADASSYFFEDTPAEAKQLLFPN 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           TQKVTLPFTGNYDKLQSIVGKYRD++ELGIPALSTAVTNMVYYNKQ TAAALLVLIQS+A
Sbjct: 121 TQKVTLPFTGNYDKLQSIVGKYRDLIELGIPALSTAVTNMVYYNKQLTAAALLVLIQSSA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIEQQVAQ+IS KFYPNQAVISLENNWGALSKQIQIANSTG GQFQSPVELISP
Sbjct: 181 EAARFKYIEQQVAQHISDKFYPNQAVISLENNWGALSKQIQIANSTGRGQFQSPVELISP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMPDNIVEYGAHDSM 293
           NGTRF VTSTS+GVVQ NIKLLLYYKLSVAD+NDSPTRMPDNIVEYG HDS+
Sbjct: 241 NGTRFSVTSTSAGVVQSNIKLLLYYKLSVADENDSPTRMPDNIVEYGTHDSV 292

[Close]
BLAST of Carg04937 vs. NCBI nr
Match: P56626.2 (RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; AltName: Full=Trichoanguin; AltName: Full=rRNA N-glycosidase; Flags: Precursor >AAD02686.1 trichoanguin [Trichosanthes cucumerina])

HSP 1 Score: 397.1 bits (1019), Expect = 5.1e-107
Identity = 203/273 (74.36%), Postives = 237/273 (86.81%), Query Frame = 0 

Query: 5   IAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLLPSTA 64
           +A++ F LAISL SPT   D++FDLSTATK SYS+FITQLR+ALPT+ TVC IPLLPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  TGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAGTQKV 124
           +G  WF FF +TNYN +TV VAVNV+NVYIVAYRADA SYFFEDTPA+A +L+FAGT+ V
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 TLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTAEAAR 184
            LP++GNYDKLQS+VGK RDM+ELGIPALS+A+TNMVYY+ Q TAAALLVLIQ TAEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 FKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISPNGTR 244
           +KYIEQQV+ +IS  FYPNQAVISLEN WGALSKQIQIAN TG GQF++PVEL +P+GTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 FRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPT 278
           F VT+TS+GVV+GNIKLLLYYK SV  + D PT
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPT 273

[Close]
BLAST of Carg04937 vs. NCBI nr
Match: XP_022156709.1 (type I ribosome-inactivating protein trichoanguina-like [Momordica charantia])

HSP 1 Score: 369.0 bits (946), Expect = 1.5e-98
Identity = 190/281 (67.62%), Postives = 233/281 (82.92%), Query Frame = 0 

Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           M K IA++ F+L I L  PT K D++FDLSTATK SY++FI  LR+ALPT+ TVCNIP+L
Sbjct: 1   MMKSIALSFFILTIFLGPPTAKGDVSFDLSTATKNSYTSFIRDLRSALPTRGTVCNIPVL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
           PSTA+GL WF FF++TNYNYQT+ VAVNV+NVYI AY   A SYFFEDT A+A +LLF G
Sbjct: 61  PSTASGLQWFRFFSLTNYNYQTITVAVNVTNVYIAAYSTGAVSYFFEDTSAEAFRLLFVG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNK-QPTAAALLVLIQST 180
           TQKVTLP++GNYD+LQSIVGK RD++ELGIP LS A+TN+ Y N  + TA ALLVLIQST
Sbjct: 121 TQKVTLPYSGNYDRLQSIVGKNRDLIELGIPTLSNAITNLFYNNNYKLTATALLVLIQST 180

Query: 181 AEAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELIS 240
           AEAARFKYIEQ+V++++S  F+PNQA+ISLEN+WGALSKQIQIANS G+GQF   VELI+
Sbjct: 181 AEAARFKYIEQEVSKHVSSNFFPNQAIISLENSWGALSKQIQIANSKGTGQFDKAVELIT 240

Query: 241 PNGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPTRMP 281
            +G R  VT+TS+GVV+ NIKLLLYYK++ A+DN  PT +P
Sbjct: 241 IDGKRVSVTNTSTGVVKSNIKLLLYYKVNGANDNYIPTILP 281

[Close]
BLAST of Carg04937 vs. Swiss-Prot
Match: sp|P56626|RIP1_TRIAN (Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=50544 GN=TCA PE=1 SV=2)

HSP 1 Score: 397.1 bits (1019), Expect = 1.7e-109
Identity = 203/273 (74.36%), Postives = 237/273 (86.81%), Query Frame = 0 

Query: 5   IAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLLPSTA 64
           +A++ F LAISL SPT   D++FDLSTATK SYS+FITQLR+ALPT+ TVC IPLLPSTA
Sbjct: 1   MALSFFFLAISLGSPTAIGDVSFDLSTATKKSYSSFITQLRDALPTQGTVCGIPLLPSTA 60

Query: 65  TGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAGTQKV 124
           +G  WF FF +TNYN +TV VAVNV+NVYIVAYRADA SYFFEDTPA+A +L+FAGT+ V
Sbjct: 61  SGSQWFRFFNLTNYNDETVTVAVNVTNVYIVAYRADAVSYFFEDTPAEAFKLIFAGTKTV 120

Query: 125 TLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTAEAAR 184
            LP++GNYDKLQS+VGK RDM+ELGIPALS+A+TNMVYY+ Q TAAALLVLIQ TAEAAR
Sbjct: 121 KLPYSGNYDKLQSVVGKQRDMIELGIPALSSAITNMVYYDYQSTAAALLVLIQCTAEAAR 180

Query: 185 FKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISPNGTR 244
           +KYIEQQV+ +IS  FYPNQAVISLEN WGALSKQIQIAN TG GQF++PVEL +P+GTR
Sbjct: 181 YKYIEQQVSSHISSNFYPNQAVISLENKWGALSKQIQIANRTGHGQFENPVELYNPDGTR 240

Query: 245 FRVTSTSSGVVQGNIKLLLYYKLSVADDNDSPT 278
           F VT+TS+GVV+GNIKLLLYYK SV  + D PT
Sbjct: 241 FSVTNTSAGVVKGNIKLLLYYKASVGSEYDIPT 273

[Close]
BLAST of Carg04937 vs. Swiss-Prot
Match: sp|P24817|RIP3_MOMCH (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 272.3 bits (695), Expect = 6.2e-72
Identity = 142/273 (52.01%), Postives = 196/273 (71.79%), Query Frame = 0 

Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           M K + ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP    V +IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
            ST +    F    +T+Y Y+T+ VA++V+NVY+VAYR    SYFF+++P +A  +LF G
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           T+K+TLP+TGNY+ LQ+   K R+ ++LG+PALS+A+T + YYN Q   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIE+ VA+Y++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDN 274
            G RF+VT+  S VV+GNIKLLL  + S AD+N
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 272

[Close]
BLAST of Carg04937 vs. Swiss-Prot
Match: sp|P29339|RIP2_MOMBA (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 8.1e-72
Identity = 142/273 (52.01%), Postives = 196/273 (71.79%), Query Frame = 0 

Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           M K + ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP    V +IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
            ST +    F    +T+Y Y+T+ VA++V+NVY+VAYR    SYFF+++P +A  +LF G
Sbjct: 61  YSTISDSRRFILLDLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           T+K+TLP+TGNY+ LQ+   K R+ ++LG+PALS+A+T + YYN Q   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIE+ VA+Y++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDN 274
            G RF+VT+  S VV+GNIKLLL  + S AD+N
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 272

[Close]
BLAST of Carg04937 vs. Swiss-Prot
Match: sp|P84531|RIP_CUCMO (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 243.8 bits (621), Expect = 2.3e-63
Identity = 127/240 (52.92%), Postives = 170/240 (70.83%), Query Frame = 0 

Query: 24  DIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLLPSTATGLGWFTFFTITNYNYQTV 83
           ++ FDLS+AT +SY  FI  LR ALP    V +IP+L ST      F    + NY+ Q++
Sbjct: 1   NVRFDLSSATSSSYKTFIKNLREALPKDGKVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 60

Query: 84  EVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAGTQKVTLPFTGNYDKLQSIVGKYR 143
             A++V NVYIVAY     SYFF+  PAQA +LLF GTQ+ TLP+TGNY+ LQ+   K R
Sbjct: 61  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKLR 120

Query: 144 DMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTAEAARFKYIEQQVAQYISGKFYPN 203
           + +ELG+PAL +A+T + +YN +  A+ALLVLIQ+T+EAARF+YIE Q+A  +  KF P+
Sbjct: 121 ENIELGLPALDSAITTLFHYNAEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFKPS 180

Query: 204 QAVISLENNWGALSKQIQIANSTGSGQFQSPVELISPNGTRFRVTSTSSGVVQGNIKLLL 263
           Q +ISLENNW ALSKQIQIA +  +GQF++PV LI P G R ++T+ +S VV  NI+LLL
Sbjct: 181 QTIISLENNWSALSKQIQIAKNK-NGQFETPVILIDPQGNRVQITNVTSNVVTQNIQLLL 239

[Close]
BLAST of Carg04937 vs. Swiss-Prot
Match: sp|P09989|RIPT_TRIKI (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 239.2 bits (609), Expect = 5.8e-62
Identity = 129/278 (46.40%), Postives = 186/278 (66.91%), Query Frame = 0 

Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           M + + ++  +L + L +P ++ D++F LS AT +SY  FI+ LR ALP +  + +IPLL
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPA--QAKQLLF 120
            S+  G   +    +TNY  +T+ VA++V+NVYI+ YRA   SYFF +  A   AK +  
Sbjct: 61  RSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFK 120

Query: 121 AGTQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQS 180
              +KVTLP++GNY++LQ+  GK R+ + LG+PAL +A+T + YYN    A+AL+VLIQS
Sbjct: 121 DAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANSAASALMVLIQS 180

Query: 181 TAEAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELI 240
           T+EAAR+K+IEQQ+ + +   F P+ A+ISLEN+W ALSKQIQIA ST +GQF+SPV LI
Sbjct: 181 TSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIA-STNNGQFESPVVLI 240

Query: 241 SPNGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDNDSP 277
           +    R  +T+  +GVV  NI LLL      A D+D P
Sbjct: 241 NAQNQRVTITNVDAGVVTSNIALLLNRNNMAAMDDDVP 277

[Close]
BLAST of Carg04937 vs. TrEMBL
Match: tr|S6CNA6|S6CNA6_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=4 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 5.6e-70
Identity = 141/267 (52.81%), Postives = 194/267 (72.66%), Query Frame = 0 

Query: 7   ITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLLPSTATG 66
           ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP    V +IPLL ST + 
Sbjct: 1   LSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLLYSTISD 60

Query: 67  LGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAGTQKVTL 126
              F    +T+Y Y+T+ VA++V+NVY+VAYR    SYFF+++P +A  +LF GT+K+TL
Sbjct: 61  SRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITL 120

Query: 127 PFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTAEAARFK 186
           P+TGNY+ LQ+   K R+ ++LG+PALS+A+T + YYN QP  +ALLVLIQ+TAEAARFK
Sbjct: 121 PYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQPAPSALLVLIQTTAEAARFK 180

Query: 187 YIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISPNGTRFR 246
           YIE+ VA+Y++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P G RF+
Sbjct: 181 YIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQ 240

Query: 247 VTSTSSGVVQGNIKLLLYYKLSVADDN 274
           VT+  S VV+GNIKLLL  + S AD+N
Sbjct: 241 VTNVDSDVVKGNIKLLLNSRASTADEN 266

[Close]
BLAST of Carg04937 vs. TrEMBL
Match: tr|S6CNA0|S6CNA0_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=4 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 7.3e-70
Identity = 141/267 (52.81%), Postives = 194/267 (72.66%), Query Frame = 0 

Query: 7   ITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLLPSTATG 66
           ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP    V +IPLL ST +G
Sbjct: 1   LSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLLYSTISG 60

Query: 67  LGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAGTQKVTL 126
              F    +T+Y Y+T+ VA++V+NVY+VAYR    SYFF+++P +A  +LF GT+K+TL
Sbjct: 61  SRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITL 120

Query: 127 PFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTAEAARFK 186
           P+TGNY+ LQ+   K R+ ++LG+PALS+A+T + YYN Q   +ALLVLIQ+TAEAARFK
Sbjct: 121 PYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTAEAARFK 180

Query: 187 YIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISPNGTRFR 246
           YIE+ VA+Y++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P G RF+
Sbjct: 181 YIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQ 240

Query: 247 VTSTSSGVVQGNIKLLLYYKLSVADDN 274
           VT+  S VV+GNIKLLL  + S AD+N
Sbjct: 241 VTNVDSDVVKGNIKLLLNSRASTADEN 266

[Close]
BLAST of Carg04937 vs. TrEMBL
Match: tr|W0LQW1|W0LQW1_MOMCH (rRNA N-glycosidase OS=Momordica charantia OX=3673 PE=2 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 9.5e-70
Identity = 142/273 (52.01%), Postives = 196/273 (71.79%), Query Frame = 0 

Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           M K + ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP    V +IPLL
Sbjct: 1   MVKCLVLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIENFRATLPFSHKVYDIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
            ST +    F    +T+Y Y+T+ VA++V+NVY+VAYR    SYFF+++P +A  +LF G
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           T+K+TLP+TGNY+ LQ+   K R+ ++LG+PALS+A+T + YYN Q   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIE+ VA+Y++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P
Sbjct: 181 EAARFKYIERHVARYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDN 274
            G RF+VT+  S VV+GNIKLLL  + S AD+N
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 272

[Close]
BLAST of Carg04937 vs. TrEMBL
Match: tr|Q5PZ05|Q5PZ05_MOMCH (rRNA N-glycosidase OS=Momordica charantia OX=3673 PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.2e-69
Identity = 142/273 (52.01%), Postives = 196/273 (71.79%), Query Frame = 0 

Query: 1   MNKLIAITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLL 60
           M K + ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP    V +IPLL
Sbjct: 1   MVKCLLLSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLL 60

Query: 61  PSTATGLGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAG 120
            ST +    F    +T+Y Y+T+ VA++V+NVY+VAYR    SYFF+++P +A  +LF G
Sbjct: 61  YSTISDSRRFILLNLTSYAYETISVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKG 120

Query: 121 TQKVTLPFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTA 180
           T+K+TLP+TGNY+ LQ+   K R+ ++LG+PALS+A+T + YYN Q   +ALLVLIQ+TA
Sbjct: 121 TRKITLPYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTA 180

Query: 181 EAARFKYIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISP 240
           EAARFKYIE+ VA+Y++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P
Sbjct: 181 EAARFKYIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKP 240

Query: 241 NGTRFRVTSTSSGVVQGNIKLLLYYKLSVADDN 274
            G RF+VT+  S VV+GNIKLLL  + S AD+N
Sbjct: 241 TGERFQVTNVDSDVVKGNIKLLLNSRASTADEN 272

[Close]
BLAST of Carg04937 vs. TrEMBL
Match: tr|S6CM68|S6CM68_MOMCH (Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.6e-69
Identity = 141/267 (52.81%), Postives = 193/267 (72.28%), Query Frame = 0 

Query: 7   ITSFLLAISLISPTLKADIAFDLSTATKASYSAFITQLRNALPTKATVCNIPLLPSTATG 66
           ++  ++AI +  PT K D+ FDLSTAT  +Y+ FI   R  LP    V +IPLL ST + 
Sbjct: 1   LSFLIIAIFIGVPTAKGDVNFDLSTATAKTYTKFIEDFRATLPFSHKVYDIPLLYSTISD 60

Query: 67  LGWFTFFTITNYNYQTVEVAVNVSNVYIVAYRADANSYFFEDTPAQAKQLLFAGTQKVTL 126
              F    +T+Y Y+T+ VAV+V+NVY+VAYR    SYFF+++P +A  +LF GT+K+TL
Sbjct: 61  SRRFILLNLTSYAYETISVAVDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITL 120

Query: 127 PFTGNYDKLQSIVGKYRDMVELGIPALSTAVTNMVYYNKQPTAAALLVLIQSTAEAARFK 186
           P+TGNY+ LQ+   K R+ ++LG+PALS+A+T + YYN Q   +ALLVLIQ+TAEAARFK
Sbjct: 121 PYTGNYENLQTAAHKIRENIDLGLPALSSAITTLFYYNAQSAPSALLVLIQTTAEAARFK 180

Query: 187 YIEQQVAQYISGKFYPNQAVISLENNWGALSKQIQIANSTGSGQFQSPVELISPNGTRFR 246
           YIE+ VA+Y++  F PN A+ISLEN W ALSKQI +A + G G+F++PV+LI P G RF+
Sbjct: 181 YIERHVAKYVATNFKPNLAIISLENQWSALSKQIFLAQNQG-GKFRNPVDLIKPTGERFQ 240

Query: 247 VTSTSSGVVQGNIKLLLYYKLSVADDN 274
           VT+  S VV+GNIKLLL  + S AD+N
Sbjct: 241 VTNVDSDVVKGNIKLLLNSRASTADEN 266

The following BLAST results are available for this feature:
BLAST of Carg04937 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022960460.15.0e-14794.18type I ribosome-inactivating protein trichoanguina-like [Cucurbita moschata][more]
XP_023513430.11.4e-14493.49type I ribosome-inactivating protein trichoanguina-like [Cucurbita pepo subsp. p... [more]
XP_023005055.12.3e-14491.78type I ribosome-inactivating protein trichoanguina-like [Cucurbita maxima][more]
P56626.25.1e-10774.36RecName: Full=Type I ribosome-inactivating protein trichoanguina; Short=RIP; Alt... [more]
XP_022156709.11.5e-9867.62type I ribosome-inactivating protein trichoanguina-like [Momordica charantia][more]
BLAST of Carg04937 vs. TAIR10
Match NameE-valueIdentityDescription
BLAST of Carg04937 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|P56626|RIP1_TRIAN1.7e-10974.36Type I ribosome-inactivating protein trichoanguina OS=Trichosanthes anguina OX=5... [more]
sp|P24817|RIP3_MOMCH6.2e-7252.01Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN... [more]
sp|P29339|RIP2_MOMBA8.1e-7252.01Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV... [more]
sp|P84531|RIP_CUCMO2.3e-6352.92Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2[more]
sp|P09989|RIPT_TRIKI5.8e-6246.40Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=... [more]
BLAST of Carg04937 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|S6CNA6|S6CNA6_MOMCH5.6e-7052.81Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip P... [more]
tr|S6CNA0|S6CNA0_MOMCH7.3e-7052.81Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip P... [more]
tr|W0LQW1|W0LQW1_MOMCH9.5e-7052.01rRNA N-glycosidase OS=Momordica charantia OX=3673 PE=2 SV=1[more]
tr|Q5PZ05|Q5PZ05_MOMCH1.2e-6952.01rRNA N-glycosidase OS=Momordica charantia OX=3673 PE=3 SV=1[more]
tr|S6CM68|S6CM68_MOMCH3.6e-6952.81Ribosome inactivating protein (Fragment) OS=Momordica charantia OX=3673 GN=rip P... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0017148negative regulation of translation
Vocabulary: Molecular Function
TermDefinition
GO:0030598rRNA N-glycosylase activity
Vocabulary: INTERPRO
TermDefinition
IPR036041Ribosome-inact_prot_sf
IPR017988Ribosome_inactivat_prot_CS
IPR016139Ribosome_inactivat_prot_sub2
IPR016138Ribosome_inactivat_prot_sub1
IPR001574Ribosome_inactivat_prot
IPR017989Ribosome_inactivat_1/2
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0017148 negative regulation of translation
cellular_component GO:0005575 cellular_component
molecular_function GO:0030598 rRNA N-glycosylase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04937-RACarg04937-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017989Ribosome-inactivating protein type 1/2PRINTSPR00396SHIGARICINcoord: 172..192
score: 66.67
coord: 82..97
score: 44.74
coord: 143..157
score: 40.7
coord: 204..221
score: 52.63
coord: 35..48
score: 45.3
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 33..221
e-value: 1.4E-32
score: 112.8
IPR016138Ribosome-inactivating protein, subdomain 1GENE3DG3DSA:3.40.420.10coord: 23..183
e-value: 8.7E-47
score: 160.8
IPR016139Ribosome-inactivating protein, subdomain 2GENE3DG3DSA:4.10.470.10coord: 184..270
e-value: 1.4E-27
score: 97.4
NoneNo IPR availablePANTHERPTHR33453FAMILY NOT NAMEDcoord: 24..231
IPR017988Ribosome-inactivating protein conserved sitePROSITEPS00275SHIGA_RICINcoord: 176..192
IPR036041Ribosome-inactivating protein superfamilySUPERFAMILYSSF56371Ribosome inactivating proteins (RIP)coord: 24..268