Carg04850 (gene) Silver-seed gourd

NameCarg04850
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionERAD-associated E3 ubiquitin-protein ligase component HRD3A
LocationCucurbita_argyrosperma_scaffold_028 : 1303995 .. 1307715 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATCTTTCTCGCAAATTCTACAGAGCCTTCTTTTTCTTCTTCCTTCTTCCTTCTTCCTCCTTCTTCTTCGTGTTCTTGAAAAATTCAATCCCAATGCAGATTAGGATGCAACTGCAAGCTCGTAGGCTTCAGTTAATTTTCTTAATTATATGTCTATGTTCTTTTTTCATCAACGCTCGTCCGTTTCTTATTGTCATCTCTCAAGATGACCTCAAGGATGCTGCGTCGCCCGACGACTCCTCCGATTCAGCCAACTACGACTCCGCCGAGTGGGACGAGTTTGGGGAGCCTGAATCTCAAAACGCCGCCCAAGAGCTTGACCCCGGTACTTGGCGCCCGATTTTTGAACCGGATTCTCCCGCCTCTGACCCCGACGCGCCGGAGGATCTGTACTACACTGCGTTAGGGAAGATGATGACGGCGGTGAGCTCTGGTGACCTGAGGTTGATGGAGGATGCGGTAGGGGATATTGATCAAGTTGCGGTGGAGAGTGGGGATCCGCACGCGCAATCGGTGCTAGGGTTATTGTATGGAATGGGGATAATGAAGGAGACGAATAAGGCTAAGGCGTTTATGTATCATTATTTTGCCGCGGAAGGGAATAAGCAATCTAAGATGGCTCTTGCATATAGTTACTTCAGGCAAGAAGTAAGTGCGACCCTGCGAGCACTTTAGTTATTGAATTGTGTTTAATTAATGAAGAAATTGTTGTTTTATGCGTAGGCATATATGGATTTGTTGTTCTGTTACTTGTCGTGTTTCCAATGCGTAATTCTGTCTTGGTTGCAGATGTATGAAAAAGCAGTCAAGCTTTATGCTGAGTTAGCAGAGGTAGCCATTAATAGTTTATTAGTTTCCAAGGATTCTCCAGTAATTGAACCTGTGAGGATCCATAATGGTGCAGAGGAGAACAAGCAGGCCTTGAGAAAGTCTCGAGGAGAGGAGGATGAGGACTTCCAAATTTTGGAATATCAGGCGCAGAAGGGTAATGCTGGAGCCATGTATAGAATTGGGCTATTTTACTACTTTGGACTGAGAGGGTTGAGGCGTGATCATGCAAAAGCACTGATCTGGTTTTCCAAGGCCGTGGATAAAGGTGAACCAAAGTCTATGGAACTACTTGGAGAGATATATGCAAGGGGCGCCGGAGTCGAAAGGGATTATACTAAGGCACTTCAATGGCTAACTCGTGCATCCAAGCATCCATCATTTTCTGCTTATAATGGCATTGGATATTTATACGTTAAGGGTTATGGAGTGGAGAAAAACTTTACCAAGGTAAGGTTCCCATTATTTCTGTTGAAATTGCTATTTTGACTTCGAATTTCTGTCTCCATATGTTGCTGTAATTCTTGCCTCGGAGCTGACTTAATTAGATACCATTAACTTAACCCACTATACCTCAGGCGTTACATTTTCCTGAATCATAAGTGAACTAGAGACTATTTGATTTAATGGTGTATTCAATTCGTATGGCAAGCTGAACGGTTGTTAGTCTCTTGTGCCCGTAGAAATCTGGTGGCTTATTTTCGTTTTTATGTTCTATTAGTTGAATTGCGCTTACCTATCAATTGTTGTCACCCTACCTTAAATGTGTGTATTTTAGTGGTTGTCTCTTGTTCTACTGACTGTTCATAGTAGTATACTATATTCTCAGAAGTTCTGTGCTTATCTTAGATTGTTTTAGCTTTATTATTTTGTCAGCCCCTAACTTCTGTTACCTTAAAAGATAGTTTTTTTTTTTTTTTTTTCCTTTCTTTTTTGCAATGTCTGCCCTTTAGAATCTCTGATGGGCATTGAATTTTTCTTGGAGGATGTAACATGATCTTGCTCCCATTTTACCACCACCACATGCCCTAGTCGTTGCAAGATTTGGATCTTAATGCAACTTTTGAATGCATATCCTTTTCTTTGTATTTTCATGTTCTAAATGTTGTTTTCTTCAGTATATGTTGATATGGCACCTGAATTACTGCTTGTTTCATTTTTTTTCCCACTTCAATTCTCTGCCTAAATCGTGTATAACATACGTCATCATTGCATGTTCCCCACATGCTACGTCCTCTAATACAGGCCAAGGAGTACTTTGAAAAGGCTGCCAATAACGACGAGTCTGGTGGTCATTATAATCTAGGAGTTATGTATCTTAAAGGAATTGGGGTAAAGAGAGATGTGAAGAAGGCATGTACTCATTTTATAGTGGCTGCAAATGCTGGACAACCAAAGGCATTCTACCAGCTGGCGAAGATGTTTCATACTGGTGTTGGGCTCAAGAGGAACATTCCTATGGTAAAATTCTACGTCAAGTACATTTTTCAAACCCCCCCCCGCCCCCCCCCCCGGTCTCACTTTCTCACCATGGAAAAGGAGGGAAAAGAAAACTTTTTTTGGGATCTAATAATTGAAAGTCTTCGTTTTCTGGAGCAAACGTTTTATTAAAAATAAAATCTGAACTTATTATTGGGATCCTAATAGACCACAGATTCTGTAGATAATTGAAGTTTCAACCGTTGCAGGCTAGTGCTTTATACAAATTAGTAGCTGAACGAGGGCCTTGGAGTTCATTGTCTAGATGGGCATTGGAATCATATCTGAAAAGTGATATTGGCAAGGCATTCTTCTTGTACGCGAGAATGGCTGAGCTAGGATATGAGGTGGCACAAAGCAATGCAGCATGGATACTTGACAAATATGGAGAACAAAGCATGTGTCTGGGAGAATCTGGCTTTTGCACAGATGCAGAACTACATCAGAGAGCTCATTCTCTTTGGTGGCAAGCATCCGAGCAGGGCAACGAACATGCTGCCTTACTGATTGGGGATGCATATTACTACGGTCGGGTATGTACTGAGAGTATTTCTTTTGAACCACAAATGTTCCAAAATCACGTGTTTGAGATTTTTCATTTTTCTACGTTTCCATTTCCACTCAGGGAACCGACGTAGATTATGATCGTGCTGCTGAAGCATACATGCATGCTAAATCCCAACTAAATGCACAAGCCATGTTCAACCTTGGTTACATGCATGAACATGGTCTAGGCCTTCCGTTTGACCTTCACCTAGCCAAGCGTTACTATGACCAAGCTCTGGAACTTGATCCAGCTGCCAGGTTGCCAGTCAAGCTGGCCCTAGCAAGCCTGTGGTTAAGGAAGAATCATGCCGACAGTTTTCTGGTGGGTTCAATGAACTTCTTTTCTTAATTCTTTTCTTGCAATAGTCCATTTTCCTTCCTTATTATAGACGACCTGGATATCAACTGAGGGTGTTTCTGATGTGTGAATTAGGTCCATGTGATCGATTCGTTGCCAGAAGTGTATCCAAAGATTGATGTATGGGTGGAGGATGTACTGTTGGAGGAAGGAAATGCAACAATTCTAACTCTATTTGCCTGCCTCCTCACTGTCCTATATCTTAGAGAGCGGCAACGGAGGCATGCAGTTGTTCGGGCTGCTGCTGCTGCTGCTGCTGCTGAGGCTGTGCCACTCCACCCCAATGATCACGTGGCTGCACAAAATTAAATCACTGCCTGTGTTGTCTTTTTCTTTTCAATGAGGTATGAGAATCTTTTCTTTAACGTTATGTACAGGAAATTAGAATTCAACTGCTTAGAGAGAGCTTTTGCGTTCTTGGGACTTTCACAACACAGAGTAGAGGAAGAACTTCAAGATGTTTATCACCGGGATCATAGAAAGCAAAGATTTTTATATATTTTTTTTCTG

mRNA sequence

GATCTTTCTCGCAAATTCTACAGAGCCTTCTTTTTCTTCTTCCTTCTTCCTTCTTCCTCCTTCTTCTTCGTGTTCTTGAAAAATTCAATCCCAATGCAGATTAGGATGCAACTGCAAGCTCGTAGGCTTCAGTTAATTTTCTTAATTATATGTCTATGTTCTTTTTTCATCAACGCTCGTCCGTTTCTTATTGTCATCTCTCAAGATGACCTCAAGGATGCTGCGTCGCCCGACGACTCCTCCGATTCAGCCAACTACGACTCCGCCGAGTGGGACGAGTTTGGGGAGCCTGAATCTCAAAACGCCGCCCAAGAGCTTGACCCCGGTACTTGGCGCCCGATTTTTGAACCGGATTCTCCCGCCTCTGACCCCGACGCGCCGGAGGATCTGTACTACACTGCGTTAGGGAAGATGATGACGGCGGTGAGCTCTGGTGACCTGAGGTTGATGGAGGATGCGGTAGGGGATATTGATCAAGTTGCGGTGGAGAGTGGGGATCCGCACGCGCAATCGGTGCTAGGGTTATTGTATGGAATGGGGATAATGAAGGAGACGAATAAGGCTAAGGCGTTTATGTATCATTATTTTGCCGCGGAAGGGAATAAGCAATCTAAGATGGCTCTTGCATATAGTTACTTCAGGCAAGAAATGTATGAAAAAGCAGTCAAGCTTTATGCTGAGTTAGCAGAGGTAGCCATTAATAGTTTATTAGTTTCCAAGGATTCTCCAGTAATTGAACCTGTGAGGATCCATAATGGTGCAGAGGAGAACAAGCAGGCCTTGAGAAAGTCTCGAGGAGAGGAGGATGAGGACTTCCAAATTTTGGAATATCAGGCGCAGAAGGGTAATGCTGGAGCCATGTATAGAATTGGGCTATTTTACTACTTTGGACTGAGAGGGTTGAGGCGTGATCATGCAAAAGCACTGATCTGGTTTTCCAAGGCCGTGGATAAAGGTGAACCAAAGTCTATGGAACTACTTGGAGAGATATATGCAAGGGGCGCCGGAGTCGAAAGGGATTATACTAAGGCACTTCAATGGCTAACTCGTGCATCCAAGCATCCATCATTTTCTGCTTATAATGGCATTGGATATTTATACGTTAAGGGTTATGGAGTGGAGAAAAACTTTACCAAGGCCAAGGAGTACTTTGAAAAGGCTGCCAATAACGACGAGTCTGGTGGTCATTATAATCTAGGAGTTATGTATCTTAAAGGAATTGGGGTAAAGAGAGATGTGAAGAAGGCATGTACTCATTTTATAGTGGCTGCAAATGCTGGACAACCAAAGGCATTCTACCAGCTGGCGAAGATGTTTCATACTGGTGTTGGGCTCAAGAGGAACATTCCTATGGCTAGTGCTTTATACAAATTAGTAGCTGAACGAGGGCCTTGGAGTTCATTGTCTAGATGGGCATTGGAATCATATCTGAAAAGTGATATTGGCAAGGCATTCTTCTTGTACGCGAGAATGGCTGAGCTAGGATATGAGGTGGCACAAAGCAATGCAGCATGGATACTTGACAAATATGGAGAACAAAGCATGTGTCTGGGAGAATCTGGCTTTTGCACAGATGCAGAACTACATCAGAGAGCTCATTCTCTTTGGTGGCAAGCATCCGAGCAGGGCAACGAACATGCTGCCTTACTGATTGGGGATGCATATTACTACGGTCGGGGAACCGACGTAGATTATGATCGTGCTGCTGAAGCATACATGCATGCTAAATCCCAACTAAATGCACAAGCCATGTTCAACCTTGGTTACATGCATGAACATGGTCTAGGCCTTCCGTTTGACCTTCACCTAGCCAAGCGTTACTATGACCAAGCTCTGGAACTTGATCCAGCTGCCAGGTTGCCAGTCAAGCTGGCCCTAGCAAGCCTGTGGTTAAGGAAGAATCATGCCGACAGTTTTCTGGTCCATGTGATCGATTCGTTGCCAGAAGTGTATCCAAAGATTGATGTATGGGTGGAGGATGTACTGTTGGAGGAAGGAAATGCAACAATTCTAACTCTATTTGCCTGCCTCCTCACTGTCCTATATCTTAGAGAGCGGCAACGGAGGCATGCAGTTGTTCGGGCTGCTGCTGCTGCTGCTGCTGCTGAGGCTGTGCCACTCCACCCCAATGATCACGTGGCTGCACAAAATTAAATCACTGCCTGTGTTGTCTTTTTCTTTTCAATGAGGTATGAGAATCTTTTCTTTAACGTTATGTACAGGAAATTAGAATTCAACTGCTTAGAGAGAGCTTTTGCGTTCTTGGGACTTTCACAACACAGAGTAGAGGAAGAACTTCAAGATGTTTATCACCGGGATCATAGAAAGCAAAGATTTTTATATATTTTTTTTCTG

Coding sequence (CDS)

GATCTTTCTCGCAAATTCTACAGAGCCTTCTTTTTCTTCTTCCTTCTTCCTTCTTCCTCCTTCTTCTTCGTGTTCTTGAAAAATTCAATCCCAATGCAGATTAGGATGCAACTGCAAGCTCGTAGGCTTCAGTTAATTTTCTTAATTATATGTCTATGTTCTTTTTTCATCAACGCTCGTCCGTTTCTTATTGTCATCTCTCAAGATGACCTCAAGGATGCTGCGTCGCCCGACGACTCCTCCGATTCAGCCAACTACGACTCCGCCGAGTGGGACGAGTTTGGGGAGCCTGAATCTCAAAACGCCGCCCAAGAGCTTGACCCCGGTACTTGGCGCCCGATTTTTGAACCGGATTCTCCCGCCTCTGACCCCGACGCGCCGGAGGATCTGTACTACACTGCGTTAGGGAAGATGATGACGGCGGTGAGCTCTGGTGACCTGAGGTTGATGGAGGATGCGGTAGGGGATATTGATCAAGTTGCGGTGGAGAGTGGGGATCCGCACGCGCAATCGGTGCTAGGGTTATTGTATGGAATGGGGATAATGAAGGAGACGAATAAGGCTAAGGCGTTTATGTATCATTATTTTGCCGCGGAAGGGAATAAGCAATCTAAGATGGCTCTTGCATATAGTTACTTCAGGCAAGAAATGTATGAAAAAGCAGTCAAGCTTTATGCTGAGTTAGCAGAGGTAGCCATTAATAGTTTATTAGTTTCCAAGGATTCTCCAGTAATTGAACCTGTGAGGATCCATAATGGTGCAGAGGAGAACAAGCAGGCCTTGAGAAAGTCTCGAGGAGAGGAGGATGAGGACTTCCAAATTTTGGAATATCAGGCGCAGAAGGGTAATGCTGGAGCCATGTATAGAATTGGGCTATTTTACTACTTTGGACTGAGAGGGTTGAGGCGTGATCATGCAAAAGCACTGATCTGGTTTTCCAAGGCCGTGGATAAAGGTGAACCAAAGTCTATGGAACTACTTGGAGAGATATATGCAAGGGGCGCCGGAGTCGAAAGGGATTATACTAAGGCACTTCAATGGCTAACTCGTGCATCCAAGCATCCATCATTTTCTGCTTATAATGGCATTGGATATTTATACGTTAAGGGTTATGGAGTGGAGAAAAACTTTACCAAGGCCAAGGAGTACTTTGAAAAGGCTGCCAATAACGACGAGTCTGGTGGTCATTATAATCTAGGAGTTATGTATCTTAAAGGAATTGGGGTAAAGAGAGATGTGAAGAAGGCATGTACTCATTTTATAGTGGCTGCAAATGCTGGACAACCAAAGGCATTCTACCAGCTGGCGAAGATGTTTCATACTGGTGTTGGGCTCAAGAGGAACATTCCTATGGCTAGTGCTTTATACAAATTAGTAGCTGAACGAGGGCCTTGGAGTTCATTGTCTAGATGGGCATTGGAATCATATCTGAAAAGTGATATTGGCAAGGCATTCTTCTTGTACGCGAGAATGGCTGAGCTAGGATATGAGGTGGCACAAAGCAATGCAGCATGGATACTTGACAAATATGGAGAACAAAGCATGTGTCTGGGAGAATCTGGCTTTTGCACAGATGCAGAACTACATCAGAGAGCTCATTCTCTTTGGTGGCAAGCATCCGAGCAGGGCAACGAACATGCTGCCTTACTGATTGGGGATGCATATTACTACGGTCGGGGAACCGACGTAGATTATGATCGTGCTGCTGAAGCATACATGCATGCTAAATCCCAACTAAATGCACAAGCCATGTTCAACCTTGGTTACATGCATGAACATGGTCTAGGCCTTCCGTTTGACCTTCACCTAGCCAAGCGTTACTATGACCAAGCTCTGGAACTTGATCCAGCTGCCAGGTTGCCAGTCAAGCTGGCCCTAGCAAGCCTGTGGTTAAGGAAGAATCATGCCGACAGTTTTCTGGTCCATGTGATCGATTCGTTGCCAGAAGTGTATCCAAAGATTGATGTATGGGTGGAGGATGTACTGTTGGAGGAAGGAAATGCAACAATTCTAACTCTATTTGCCTGCCTCCTCACTGTCCTATATCTTAGAGAGCGGCAACGGAGGCATGCAGTTGTTCGGGCTGCTGCTGCTGCTGCTGCTGCTGAGGCTGTGCCACTCCACCCCAATGATCACGTGGCTGCACAAAATTAA

Protein sequence

DLSRKFYRAFFFFFLLPSSSFFFVFLKNSIPMQIRMQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFGEPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVGDIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYSYFRQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARGAGVERDYTKALQWLTRASKHPSFSAYNGIGYLYVKGYGVEKNFTKAKEYFEKAANNDESGGHYNLGVMYLKGIGVKRDVKKACTHFIVAANAGQPKAFYQLAKMFHTGVGLKRNIPMASALYKLVAERGPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSMCLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNHADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRERQRRHAVVRAAAAAAAAEAVPLHPNDHVAAQN
BLAST of Carg04850 vs. NCBI nr
Match: XP_023001775.1 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita maxima] >XP_023001777.1 ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita maxima])

HSP 1 Score: 1016.1 bits (2626), Expect = 5.7e-293
Identity = 689/693 (99.42%), Postives = 691/693 (99.71%), Query Frame = 0

Query: 26  LKNSIPMQIRMQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSAN 85
           LKNSIPMQIRMQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSAN
Sbjct: 3   LKNSIPMQIRMQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSAN 62

Query: 86  YDSAEWDEFGEPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSG 145
           Y+SAEWDEFGEPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSG
Sbjct: 63  YESAEWDEFGEPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSG 122

Query: 146 DLRLMEDAVGDIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSK 205
           DLRLMEDAVGDIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSK
Sbjct: 123 DLRLMEDAVGDIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSK 182

Query: 206 MALAYSYFRQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSR 265
           MALAYSYFRQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSR
Sbjct: 183 MALAYSYFRQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSR 242

Query: 266 GEEDEDFQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSME 325
           GEEDEDFQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWF+KAVDKGEPKSME
Sbjct: 243 GEEDEDFQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFAKAVDKGEPKSME 302

Query: 326 LLGEIYARGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 385
           LLGEIYARGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 303 LLGEIYARGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 362

Query: 386 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 445
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 363 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 422

Query: 446 XXXXXXXXXXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAW 505
           XXXXXXXXXXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAW
Sbjct: 423 XXXXXXXXXXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAW 482

Query: 506 ILDKYGEQSMCLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDY 565
           ILDKYGEQSMCLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDY
Sbjct: 483 ILDKYGEQSMCLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDY 542

Query: 566 DRAAEAYMHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLA 625
           DRAAEAYMHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLA
Sbjct: 543 DRAAEAYMHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLA 602

Query: 626 LASLWLRKNHADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRE 685
           LASLWLRKNHADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRE
Sbjct: 603 LASLWLRKNHADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRE 662

Query: 686 -XXXXXXXXXXXXXXXXXXAVPLHPNDHVAAQN 718
            XXXXXXXXXXXXXXXXXX VPLHPNDHVAAQN
Sbjct: 663 XXXXXXXXXXXXXXXXXXXXVPLHPNDHVAAQN 695

BLAST of Carg04850 vs. NCBI nr
Match: XP_023519772.1 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1011.1 bits (2613), Expect = 1.8e-291
Identity = 684/686 (99.71%), Postives = 685/686 (99.85%), Query Frame = 0

Query: 32  MQIRMQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEW 91
           MQIRMQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEW
Sbjct: 1   MQIRMQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEW 60

Query: 92  DEFGEPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLME 151
           DEFGEPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLME
Sbjct: 61  DEFGEPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLME 120

Query: 152 DAVGDIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYS 211
           DAVGDIDQVAVESGDPHAQSVLGLLYG GIMKETNKAKAFMYHYFAAEGNKQSKMALAYS
Sbjct: 121 DAVGDIDQVAVESGDPHAQSVLGLLYGTGIMKETNKAKAFMYHYFAAEGNKQSKMALAYS 180

Query: 212 YFRQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDED 271
           YFRQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDED
Sbjct: 181 YFRQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDED 240

Query: 272 FQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIY 331
           FQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIY
Sbjct: 241 FQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIY 300

Query: 332 ARGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 391
           ARGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 301 ARGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 360

Query: 392 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 451
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 452 XXXXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYG 511
           XXXXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYG
Sbjct: 421 XXXXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYG 480

Query: 512 EQSMCLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEA 571
           EQSMCLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEA
Sbjct: 481 EQSMCLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEA 540

Query: 572 YMHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWL 631
           YMHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWL
Sbjct: 541 YMHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWL 600

Query: 632 RKNHADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLREXXXXXX 691
           RKNHADSFLVHVIDSLPEVYPKID+WVEDVLLEEGNATILTLFACLLTVLYLREXXXXXX
Sbjct: 601 RKNHADSFLVHVIDSLPEVYPKIDIWVEDVLLEEGNATILTLFACLLTVLYLREXXXXXX 660

Query: 692 XXXXXXXXXXXXAVPLHPNDHVAAQN 718
           XXXXXXXXXXXXAVPLHPNDHVAAQN
Sbjct: 661 XXXXXXXXXXXXAVPLHPNDHVAAQN 686

BLAST of Carg04850 vs. NCBI nr
Match: XP_022924200.1 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita moschata] >XP_022924201.1 ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita moschata])

HSP 1 Score: 1008.8 bits (2607), Expect = 9.0e-291
Identity = 667/686 (97.23%), Postives = 668/686 (97.38%), Query Frame = 0

Query: 32  MQIRMQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEW 91
           MQIRMQLQARR+QLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEW
Sbjct: 1   MQIRMQLQARRIQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEW 60

Query: 92  DEFGEPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLME 151
           DEFGEPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLME
Sbjct: 61  DEFGEPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLME 120

Query: 152 DAVGDIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYS 211
           DAVGDIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYS
Sbjct: 121 DAVGDIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYS 180

Query: 212 YFRQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDED 271
           YFRQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDED
Sbjct: 181 YFRQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDED 240

Query: 272 FQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIY 331
           FQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIY
Sbjct: 241 FQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIY 300

Query: 332 ARGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 391
           ARGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 301 ARGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 360

Query: 392 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 451
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 452 XXXXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYG 511
           XXXXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYG
Sbjct: 421 XXXXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYG 480

Query: 512 EQSMCLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEA 571
           EQSMCLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEA
Sbjct: 481 EQSMCLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEA 540

Query: 572 YMHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWL 631
           YMHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWL
Sbjct: 541 YMHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWL 600

Query: 632 RKNHADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLREXXXXXX 691
           RKNHADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRE      
Sbjct: 601 RKNHADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRE---RQR 660

Query: 692 XXXXXXXXXXXXAVPLHPNDHVAAQN 718
                       AVPLHPNDHVAAQN
Sbjct: 661 RHAVVRAAAAAEAVPLHPNDHVAAQN 683

BLAST of Carg04850 vs. NCBI nr
Match: XP_022994230.1 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita maxima])

HSP 1 Score: 922.2 bits (2382), Expect = 1.1e-264
Identity = 624/683 (91.36%), Postives = 640/683 (93.70%), Query Frame = 0

Query: 36  MQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFG 95
           MQL+ARRLQ+IFLI+CLCS FINARP+LIVISQDDLKD A  DD  DSAN DSAEWDEFG
Sbjct: 1   MQLKARRLQVIFLILCLCSLFINARPYLIVISQDDLKDGALADDFLDSANSDSAEWDEFG 60

Query: 96  EPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVG 155
           EPESQN+++ELDPG+WRPIFEPDSP+S  DAPEDLYYTALGK M++VSSGDLRLMEDAV 
Sbjct: 61  EPESQNSSEELDPGSWRPIFEPDSPSSASDAPEDLYYTALGKFMSSVSSGDLRLMEDAVA 120

Query: 156 DIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYSYFRQ 215
           DIDQVA  SGDPHAQSVLGLL+GMGIMKETNKAKAFMYHYFAAEGNKQSKMALAY YFRQ
Sbjct: 121 DIDQVAAGSGDPHAQSVLGLLHGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYIYFRQ 180

Query: 216 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 275
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL
Sbjct: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 240

Query: 276 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARGX 335
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKAL WFSKAV+KGEP+SMEL+GEIYARGX
Sbjct: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELVGEIYARGX 300

Query: 336 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 395
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 360

Query: 396 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 455
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 456 XXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 515
           XXXXXXXXPWSSLSRWALESYLK+DI KAFFLY RMAELGYEVAQSNAAWILDKYGEQSM
Sbjct: 421 XXXXXXXXPWSSLSRWALESYLKADIRKAFFLYTRMAELGYEVAQSNAAWILDKYGEQSM 480

Query: 516 CLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 575
           CLGE GFC  AE HQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA
Sbjct: 481 CLGEYGFCAKAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 540

Query: 576 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNH 635
           KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLAL SLWLRKNH
Sbjct: 541 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALGSLWLRKNH 600

Query: 636 ADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLREXXXXXXXXXX 695
           AD FLVHVIDSLPEVYPKID WVEDVLLEEGNATILTLFACLLTVLYLRE          
Sbjct: 601 ADCFLVHVIDSLPEVYPKIDAWVEDVLLEEGNATILTLFACLLTVLYLRE-----RQRRH 660

Query: 696 XXXXXXXXAVPLHPNDH-VAAQN 718
                   AVPLHPNDH VAAQN
Sbjct: 661 AAVRAAAVAVPLHPNDHAVAAQN 678

BLAST of Carg04850 vs. NCBI nr
Match: XP_023542435.1 (ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 912.5 bits (2357), Expect = 8.8e-262
Identity = 621/683 (90.92%), Postives = 637/683 (93.27%), Query Frame = 0

Query: 36  MQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFG 95
           MQL+ARRLQ+IFLI+CLCS FINARP+LIVISQDDLKD A  DD       DSAEWDEFG
Sbjct: 1   MQLKARRLQVIFLILCLCSLFINARPYLIVISQDDLKDGALADDFP-----DSAEWDEFG 60

Query: 96  EPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVG 155
           EPESQN+++ELDPG+WRPIFEPDSP+S  DAPEDLYYTALGK M++VSSGDLRLMEDAV 
Sbjct: 61  EPESQNSSEELDPGSWRPIFEPDSPSSASDAPEDLYYTALGKFMSSVSSGDLRLMEDAVA 120

Query: 156 DIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYSYFRQ 215
           DIDQVA  SGDPHAQSVLGLL+GMGIMKETNKAKAFMYHYFAAEGNKQSKMALAY YFRQ
Sbjct: 121 DIDQVAAGSGDPHAQSVLGLLHGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYIYFRQ 180

Query: 216 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 275
           EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL
Sbjct: 181 EMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQIL 240

Query: 276 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARGX 335
           EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKAL WFSKAV+KGEP+SMEL+GEIYARGX
Sbjct: 241 EYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPRSMELVGEIYARGX 300

Query: 336 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 395
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 301 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 360

Query: 396 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 455
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 456 XXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSM 515
           XXXXXXXXPWSSLSRWALESYLK+DI KAFFLY RMAELGYEVAQSNAAWILDKYGEQSM
Sbjct: 421 XXXXXXXXPWSSLSRWALESYLKADIRKAFFLYTRMAELGYEVAQSNAAWILDKYGEQSM 480

Query: 516 CLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 575
           CLGESGFC  AE HQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA
Sbjct: 481 CLGESGFCAKAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHA 540

Query: 576 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNH 635
           KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLAL SLWLRKNH
Sbjct: 541 KSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALGSLWLRKNH 600

Query: 636 ADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLREXXXXXXXXXX 695
           AD FLVHVIDSLPEVYPKID WVEDVLLEEGNATILTLFACLLTVLYLRE          
Sbjct: 601 ADCFLVHVIDSLPEVYPKIDAWVEDVLLEEGNATILTLFACLLTVLYLRE-----RQRRH 660

Query: 696 XXXXXXXXAVPLHPNDH-VAAQN 718
                   AVPLHPNDH VAAQN
Sbjct: 661 AAVRAAAVAVPLHPNDHAVAAQN 673

BLAST of Carg04850 vs. TAIR10
Match: AT1G18260.1 (HCP-like superfamily protein)

HSP 1 Score: 667.5 bits (1721), Expect = 9.0e-192
Identity = 483/643 (75.12%), Postives = 556/643 (86.47%), Query Frame = 0

Query: 45  LIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFGEPESQNAAQ 104
           L  L+     F ++ARP ++V+S DDL          D+   +S+++DEFGE E + + +
Sbjct: 11  LSLLVFSFIEFGVHARPVVLVLSNDDLNSG-----GDDNGVGESSDFDEFGESEPK-SEE 70

Query: 105 ELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVGDIDQVAVES 164
           ELDPG+WR IFEPD       +P+  YY+ L K+++A S G+ RLME+AV +I + A  +
Sbjct: 71  ELDPGSWRSIFEPDDSTVQAASPQ--YYSGLKKILSAASEGNFRLMEEAVDEI-EAASSA 130

Query: 165 GDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAE-GNKQSKMALAYSYFRQEMYEKAVK 224
           GDPHAQS++G +YG+G+M+E +K+K+F++H FAA  GN QSKMALA++Y RQ+M++KAV+
Sbjct: 131 GDPHAQSIMGFVYGIGMMREKSKSKSFLHHNFAAAGGNMQSKMALAFTYLRQDMHDKAVQ 190

Query: 225 LYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQILEYQAQKGN 284
           LYAELAE A+NS L+SKDSPV+EP RIH+G EENK ALRKSRGEEDEDFQILEYQAQKGN
Sbjct: 191 LYAELAETAVNSFLISKDSPVVEPTRIHSGTEENKGALRKSRGEEDEDFQILEYQAQKGN 250

Query: 285 AGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARG-XXXXXXXX 344
           A AMY+IGLFYYFGLRGLRRDH KAL WF KAVDKGEP+SMELLGEIYARG XXXXXXXX
Sbjct: 251 ANAMYKIGLFYYFGLRGLRRDHTKALHWFLKAVDKGEPRSMELLGEIYARGXXXXXXXXX 310

Query: 345 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 404
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 311 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXXXXXXXX 370

Query: 405 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 464
           XXXXXXXXX   XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 371 XXXXXXXXXDVRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 430

Query: 465 XPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSMCLGESGF 524
           X WSSLSRWALE+YLK D+GKA  LY+RMAE+GYEVAQSNAAWILDKYGE+SMC+G SGF
Sbjct: 431 XXWSSLSRWALEAYLKGDVGKALILYSRMAEMGYEVAQSNAAWILDKYGERSMCMGVSGF 490

Query: 525 CTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHAKSQLNAQ 584
           CTD E H+RAHSLWW+ASEQGNEHAALLIGDAYYYGRGT+ D+ RAAEAYMHAKSQ NAQ
Sbjct: 491 CTDKERHERAHSLWWRASEQGNEHAALLIGDAYYYGRGTERDFVRAAEAYMHAKSQSNAQ 550

Query: 585 AMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNHADSFLVH 644
           AMFNLGYMHEHG GLPFDLHLAKRYYD++L+ D AARLPV LALASLWLR+N+AD+ LV 
Sbjct: 551 AMFNLGYMHEHGQGLPFDLHLAKRYYDESLQSDAAARLPVTLALASLWLRRNYADTVLVR 610

Query: 645 VIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRE 686
           V+DSLPEVYPK++ W+E+V+ EEGNATILTLF CL+T+LYLRE
Sbjct: 611 VVDSLPEVYPKVETWIENVVFEEGNATILTLFVCLITILYLRE 644

BLAST of Carg04850 vs. TAIR10
Match: AT1G73570.1 (HCP-like superfamily protein)

HSP 1 Score: 577.4 bits (1487), Expect = 1.2e-164
Identity = 374/610 (61.31%), Postives = 445/610 (72.95%), Query Frame = 0

Query: 57  INARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFGEPESQNAAQELDPGTWRPIFE 116
           + ARPF++V+S +DL         +D+  Y+S+++DEFGE E + + +ELDPG+WR IFE
Sbjct: 23  VQARPFVLVLSNEDLNGG-----FNDNGAYESSDFDEFGESEPK-SEEELDPGSWRRIFE 82

Query: 117 PDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVGDIDQVAVESGDPHAQSVLGLL 176
            +       A    YY+ L K+++A S G+  LME+AV +ID  A  SGDPHAQSV+G +
Sbjct: 83  TNESTVHASASPQ-YYSGLHKILSAASEGNTTLMEEAVSEIDSSA-SSGDPHAQSVMGFV 142

Query: 177 YGMGIMKETNKAKAFMYHYFAAE-GNKQSKMALAYSYFRQEMYEKAVKLYAELAEVAINS 236
           YG+G+M+ET+++K+ ++H+FAA  GN QSKMALA+ Y RQ MY+KAV+LYAELAE A+NS
Sbjct: 143 YGIGMMRETSRSKSILHHHFAAAGGNMQSKMALAFRYLRQNMYDKAVELYAELAETAVNS 202

Query: 237 LLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQILEYQAQKGNAGAMYRIGLFYY 296
            L+SKDSP+ EPVRIH G EENK ALRKSRGEEDEDFQILEYQA+KGN+ AM++IGLFYY
Sbjct: 203 FLISKDSPMAEPVRIHIGTEENKDALRKSRGEEDEDFQILEYQAEKGNSVAMHKIGLFYY 262

Query: 297 FGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARGXXXXXXXXXXXXXXXXXXXXX 356
           FGLRGLRRDHAKAL WFSKA   G       LG +Y +G                     
Sbjct: 263 FGLRGLRRDHAKALYWFSKAEFNG-------LGYLYVKG--------------------- 322

Query: 357 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 416
                                                                 XXXXXX
Sbjct: 323 --------------YGVDKRNYTKAREYFEMAANNEDPSGHYNLGVLYLKGTGVXXXXXX 382

Query: 417 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPWSSLSRWALES 476
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX WSSLSRWALE+
Sbjct: 383 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXWSSLSRWALEA 442

Query: 477 YLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSMCLGESGFCTDAELHQRAHSL 536
           YLK D+GKAF LY+RM+ELGYEVAQSNAAWI+DKYGE+SMC+G  GFCTD E H RAHSL
Sbjct: 443 YLKGDVGKAFILYSRMSELGYEVAQSNAAWIVDKYGERSMCMGVYGFCTDKERHDRAHSL 502

Query: 537 WWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHAKSQLNAQAMFNLGYMHEHGL 596
           WW+ASEQGNEHAALLIGDAYYYGRGT+ D+ RAAEAYM+AKSQ NAQAMFNLGYMHEHG 
Sbjct: 503 WWRASEQGNEHAALLIGDAYYYGRGTERDFVRAAEAYMYAKSQSNAQAMFNLGYMHEHGE 562

Query: 597 GLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNHADSFLVHVIDSLPEVYPKID 656
           GLPFDLHLAKRYYDQAL+ D AA+LPV LALAS+W+R+N+AD+ LV V++SLPEV+ K+ 
Sbjct: 563 GLPFDLHLAKRYYDQALQSDTAAKLPVTLALASVWVRRNYADTALVQVLNSLPEVHQKVV 582

Query: 657 VWVEDVLLEE 666
            WVE+ +LEE
Sbjct: 623 EWVENGMLEE 582

BLAST of Carg04850 vs. Swiss-Prot
Match: sp|Q9LM25|HRD3A_ARATH (ERAD-associated E3 ubiquitin-protein ligase component HRD3A OS=Arabidopsis thaliana OX=3702 GN=HRD3A PE=1 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 1.6e-190
Identity = 483/643 (75.12%), Postives = 556/643 (86.47%), Query Frame = 0

Query: 45  LIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFGEPESQNAAQ 104
           L  L+     F ++ARP ++V+S DDL          D+   +S+++DEFGE E + + +
Sbjct: 11  LSLLVFSFIEFGVHARPVVLVLSNDDLNSG-----GDDNGVGESSDFDEFGESEPK-SEE 70

Query: 105 ELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVGDIDQVAVES 164
           ELDPG+WR IFEPD       +P+  YY+ L K+++A S G+ RLME+AV +I + A  +
Sbjct: 71  ELDPGSWRSIFEPDDSTVQAASPQ--YYSGLKKILSAASEGNFRLMEEAVDEI-EAASSA 130

Query: 165 GDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAE-GNKQSKMALAYSYFRQEMYEKAVK 224
           GDPHAQS++G +YG+G+M+E +K+K+F++H FAA  GN QSKMALA++Y RQ+M++KAV+
Sbjct: 131 GDPHAQSIMGFVYGIGMMREKSKSKSFLHHNFAAAGGNMQSKMALAFTYLRQDMHDKAVQ 190

Query: 225 LYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQILEYQAQKGN 284
           LYAELAE A+NS L+SKDSPV+EP RIH+G EENK ALRKSRGEEDEDFQILEYQAQKGN
Sbjct: 191 LYAELAETAVNSFLISKDSPVVEPTRIHSGTEENKGALRKSRGEEDEDFQILEYQAQKGN 250

Query: 285 AGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARG-XXXXXXXX 344
           A AMY+IGLFYYFGLRGLRRDH KAL WF KAVDKGEP+SMELLGEIYARG XXXXXXXX
Sbjct: 251 ANAMYKIGLFYYFGLRGLRRDHTKALHWFLKAVDKGEPRSMELLGEIYARGXXXXXXXXX 310

Query: 345 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 404
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 311 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXXXXXXXXXXXXXXXXXX 370

Query: 405 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 464
           XXXXXXXXX   XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 371 XXXXXXXXXDVRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 430

Query: 465 XPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSMCLGESGF 524
           X WSSLSRWALE+YLK D+GKA  LY+RMAE+GYEVAQSNAAWILDKYGE+SMC+G SGF
Sbjct: 431 XXWSSLSRWALEAYLKGDVGKALILYSRMAEMGYEVAQSNAAWILDKYGERSMCMGVSGF 490

Query: 525 CTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHAKSQLNAQ 584
           CTD E H+RAHSLWW+ASEQGNEHAALLIGDAYYYGRGT+ D+ RAAEAYMHAKSQ NAQ
Sbjct: 491 CTDKERHERAHSLWWRASEQGNEHAALLIGDAYYYGRGTERDFVRAAEAYMHAKSQSNAQ 550

Query: 585 AMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNHADSFLVH 644
           AMFNLGYMHEHG GLPFDLHLAKRYYD++L+ D AARLPV LALASLWLR+N+AD+ LV 
Sbjct: 551 AMFNLGYMHEHGQGLPFDLHLAKRYYDESLQSDAAARLPVTLALASLWLRRNYADTVLVR 610

Query: 645 VIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRE 686
           V+DSLPEVYPK++ W+E+V+ EEGNATILTLF CL+T+LYLRE
Sbjct: 611 VVDSLPEVYPKVETWIENVVFEEGNATILTLFVCLITILYLRE 644

BLAST of Carg04850 vs. Swiss-Prot
Match: sp|Q9C6B6|HRD3B_ARATH (ERAD-associated E3 ubiquitin-protein ligase component HRD3B OS=Arabidopsis thaliana OX=3702 GN=HRD3B PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 2.2e-163
Identity = 374/610 (61.31%), Postives = 445/610 (72.95%), Query Frame = 0

Query: 57  INARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFGEPESQNAAQELDPGTWRPIFE 116
           + ARPF++V+S +DL         +D+  Y+S+++DEFGE E + + +ELDPG+WR IFE
Sbjct: 23  VQARPFVLVLSNEDLNGG-----FNDNGAYESSDFDEFGESEPK-SEEELDPGSWRRIFE 82

Query: 117 PDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVGDIDQVAVESGDPHAQSVLGLL 176
            +       A    YY+ L K+++A S G+  LME+AV +ID  A  SGDPHAQSV+G +
Sbjct: 83  TNESTVHASASPQ-YYSGLHKILSAASEGNTTLMEEAVSEIDSSA-SSGDPHAQSVMGFV 142

Query: 177 YGMGIMKETNKAKAFMYHYFAAE-GNKQSKMALAYSYFRQEMYEKAVKLYAELAEVAINS 236
           YG+G+M+ET+++K+ ++H+FAA  GN QSKMALA+ Y RQ MY+KAV+LYAELAE A+NS
Sbjct: 143 YGIGMMRETSRSKSILHHHFAAAGGNMQSKMALAFRYLRQNMYDKAVELYAELAETAVNS 202

Query: 237 LLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQILEYQAQKGNAGAMYRIGLFYY 296
            L+SKDSP+ EPVRIH G EENK ALRKSRGEEDEDFQILEYQA+KGN+ AM++IGLFYY
Sbjct: 203 FLISKDSPMAEPVRIHIGTEENKDALRKSRGEEDEDFQILEYQAEKGNSVAMHKIGLFYY 262

Query: 297 FGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARGXXXXXXXXXXXXXXXXXXXXX 356
           FGLRGLRRDHAKAL WFSKA   G       LG +Y +G                     
Sbjct: 263 FGLRGLRRDHAKALYWFSKAEFNG-------LGYLYVKG--------------------- 322

Query: 357 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 416
                                                                 XXXXXX
Sbjct: 323 --------------YGVDKRNYTKAREYFEMAANNEDPSGHYNLGVLYLKGTGVXXXXXX 382

Query: 417 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPWSSLSRWALES 476
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX WSSLSRWALE+
Sbjct: 383 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXWSSLSRWALEA 442

Query: 477 YLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSMCLGESGFCTDAELHQRAHSL 536
           YLK D+GKAF LY+RM+ELGYEVAQSNAAWI+DKYGE+SMC+G  GFCTD E H RAHSL
Sbjct: 443 YLKGDVGKAFILYSRMSELGYEVAQSNAAWIVDKYGERSMCMGVYGFCTDKERHDRAHSL 502

Query: 537 WWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHAKSQLNAQAMFNLGYMHEHGL 596
           WW+ASEQGNEHAALLIGDAYYYGRGT+ D+ RAAEAYM+AKSQ NAQAMFNLGYMHEHG 
Sbjct: 503 WWRASEQGNEHAALLIGDAYYYGRGTERDFVRAAEAYMYAKSQSNAQAMFNLGYMHEHGE 562

Query: 597 GLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNHADSFLVHVIDSLPEVYPKID 656
           GLPFDLHLAKRYYDQAL+ D AA+LPV LALAS+W+R+N+AD+ LV V++SLPEV+ K+ 
Sbjct: 563 GLPFDLHLAKRYYDQALQSDTAAKLPVTLALASVWVRRNYADTALVQVLNSLPEVHQKVV 582

Query: 657 VWVEDVLLEE 666
            WVE+ +LEE
Sbjct: 623 EWVENGMLEE 582

BLAST of Carg04850 vs. Swiss-Prot
Match: sp|Q10NT7|HRD3_ORYSJ (ERAD-associated E3 ubiquitin-protein ligase component HRD3 OS=Oryza sativa subsp. japonica OX=39947 GN=HRD3 PE=2 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 3.5e-153
Identity = 426/633 (67.30%), Postives = 492/633 (77.73%), Query Frame = 0

Query: 60  RPFLIVISQDD-LKDAASPDDSSDSANYDSAEWDEFGEPESQNAAQELDPGTWRPIFEP- 119
           RPF++V+S+DD LKD A    S  SA+ DS EWD+F + ES      L P +W P+ +P 
Sbjct: 45  RPFVLVLSRDDFLKDTAGAHPSLPSADADSDEWDDF-DDESPATDPLLSPSSWVPLLDPA 104

Query: 120 -DSPASD-PDAPED-LYYTALGKMMTAVSSGDLRLMEDAVGDIDQVAVESGDPHAQSVLG 179
             SP+ D PD+P D L+   +  M++A S+GD      A   I + A   G P AQS L 
Sbjct: 105 SASPSGDEPDSPSDALFVAGVRAMLSAASAGDDAAFATAAAQI-EAAATGGHPGAQSALA 164

Query: 180 LLYGMGIMKETNKAKAFMYHYFAAE-GNKQSKMALAYSYFRQEMYEKAVKLYAELAEVAI 239
            L G G+ +  ++++AF+ H FAA+ G+ QSKMALAYS+FRQEMYE+AV LYAELAE A+
Sbjct: 165 FLSGAGMTRPASRSRAFLLHKFAADAGDLQSKMALAYSFFRQEMYEEAVTLYAELAEAAL 224

Query: 240 NSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQILEYQAQKGNAGAMYRIGLF 299
            S L+SK+ PVIEPVR+H+G EENK+ALRKSRGE+DEDFQI EYQAQ+GN  AM+++GL 
Sbjct: 225 TSSLISKEPPVIEPVRLHSGTEENKEALRKSRGEDDEDFQITEYQAQRGNTVAMHKLGLL 284

Query: 300 YYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARG-XXXXXXXXXXXXXXXXXX 359
           YY+GLRG+RRD+ KA  WFSKAV+KG+ ++MELLGEIYARG XXXXXXXXXXXXXXXXXX
Sbjct: 285 YYYGLRGVRRDYGKAYHWFSKAVEKGDTRAMELLGEIYARGXXXXXXXXXXXXXXXXXXX 344

Query: 360 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 419
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 345 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 404

Query: 420 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPWSSLSRWA 479
           XXXXXXXXX         XXXXXXXXXXXXXXXXXXXXXXXXXX       PWSSLSRWA
Sbjct: 405 XXXXXXXXXRAVNAGQPKXXXXXXXXXXXXXXXXXXXXXXXXXXKSVAERGPWSSLSRWA 464

Query: 480 LESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSMCLGESGFCTDAELHQRA 539
           LESYLK DIGKA  LY+RMA+LGYEVAQSNAAWILD+YG++S+C+GESGFCTD E H RA
Sbjct: 465 LESYLKGDIGKALLLYSRMADLGYEVAQSNAAWILDRYGDESICMGESGFCTDMERHLRA 524

Query: 540 HSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHAKSQLNAQAMFNLGYMHE 599
           H+LWWQASEQGNEHAALLIGDAYYYGRG   DY+RAAEAY                    
Sbjct: 525 HALWWQASEQGNEHAALLIGDAYYYGRGVGRDYERAAEAYXXXXXXXXXXXXXXXXXXXX 584

Query: 600 HGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNHADSFLVHVIDSLPEVYP 659
                     LAKRYYDQA+E+DPAA+LPV LAL SLW+RKN+  SFLVH IDSLPEVYP
Sbjct: 585 XXXXXXXXXXLAKRYYDQAVEVDPAAKLPVMLALTSLWIRKNYDGSFLVHFIDSLPEVYP 644

Query: 660 KIDVWVEDVLLEEGNATILTLFACLLTVLYLRE 686
            ++ WVEDVL++EGNATI TLFACL+TVLYLRE
Sbjct: 645 VVEEWVEDVLMDEGNATIFTLFACLVTVLYLRE 675

BLAST of Carg04850 vs. Swiss-Prot
Match: sp|Q9ESM7|SE1L1_MESAU (Protein sel-1 homolog 1 OS=Mesocricetus auratus OX=10036 GN=Sel1l PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 4.5e-31
Identity = 91/224 (40.62%), Postives = 135/224 (60.27%), Query Frame = 0

Query: 465 WSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSMCLGESGFCT 524
           WS     A  SY   D   A   Y  +AE GYEVAQSNAA+ILD+   ++  +GE+    
Sbjct: 555 WSERLMTAYNSYKDGDYNAAVVQYLLLAEQGYEVAQSNAAFILDQ--REATIVGEN---- 614

Query: 525 DAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAY-MHAKSQLNAQA 584
             E + RA   W +A+ QG   A + +GD ++YG GTDVDY+ A   Y + ++ Q +AQA
Sbjct: 615 --ETYPRALLHWNRAASQGYTVARIKLGDYHFYGFGTDVDYETAFIHYRLASEQQHSAQA 674

Query: 585 MFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNHADSFLVHV 644
           MFNLGYMHE GLG+  D+HLAKR+YD A E  P A++PV LAL  L +       FL ++
Sbjct: 675 MFNLGYMHEKGLGIKQDIHLAKRFYDMAAEASPDAQVPVFLALCKLGV-----VYFLQYI 734

Query: 645 ID-SLPEVYPKIDVWVEDVLLEEGNATILTLFACLL-TVLYLRE 686
            + ++ +++ ++D  ++ +L  E +  ++T+ A LL TV+  R+
Sbjct: 735 REANIRDIFTQLD--MDQLLGPEWDLYLMTIIALLLGTVIAYRQ 763

BLAST of Carg04850 vs. Swiss-Prot
Match: sp|Q9UBV2|SE1L1_HUMAN (Protein sel-1 homolog 1 OS=Homo sapiens OX=9606 GN=SEL1L PE=1 SV=3)

HSP 1 Score: 137.5 bits (345), Expect = 5.8e-31
Identity = 91/224 (40.62%), Postives = 135/224 (60.27%), Query Frame = 0

Query: 465 WSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSMCLGESGFCT 524
           WS     A  SY   D   A   Y  +AE GYEVAQSNAA+ILD+   ++  +GE+    
Sbjct: 555 WSERLMTAYNSYKDGDYNAAVIQYLLLAEQGYEVAQSNAAFILDQ--REASIVGEN---- 614

Query: 525 DAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAY-MHAKSQLNAQA 584
             E + RA   W +A+ QG   A + +GD ++YG GTDVDY+ A   Y + ++ Q +AQA
Sbjct: 615 --ETYPRALLHWNRAASQGYTVARIKLGDYHFYGFGTDVDYETAFIHYRLASEQQHSAQA 674

Query: 585 MFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNHADSFLVHV 644
           MFNLGYMHE GLG+  D+HLAKR+YD A E  P A++PV LAL  L +       FL ++
Sbjct: 675 MFNLGYMHEKGLGIKQDIHLAKRFYDMAAEASPDAQVPVFLALCKLGV-----VYFLQYI 734

Query: 645 ID-SLPEVYPKIDVWVEDVLLEEGNATILTLFACLL-TVLYLRE 686
            + ++ +++ ++D  ++ +L  E +  ++T+ A LL TV+  R+
Sbjct: 735 RETNIRDMFTQLD--MDQLLGPEWDLYLMTIIALLLGTVIAYRQ 763

BLAST of Carg04850 vs. TrEMBL
Match: tr|A0A0A0LQX2|A0A0A0LQX2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G042850 PE=4 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 6.1e-251
Identity = 610/684 (89.18%), Postives = 616/684 (90.06%), Query Frame = 0

Query: 36  MQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFG 95
           MQLQ RRLQLIFLI+CL S FINARPFLIVISQDDLKD A PDDSSDSAN DSA+WDEFG
Sbjct: 1   MQLQTRRLQLIFLILCLSSLFINARPFLIVISQDDLKDGAPPDDSSDSANSDSADWDEFG 60

Query: 96  --EPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDA 155
                                           AP+DLYYTALGKMM+AVSSGDLRLMEDA
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPQDLYYTALGKMMSAVSSGDLRLMEDA 120

Query: 156 VGDIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYSYF 215
           V DIDQ   ESGDPHAQSVLGLLYGMGIMKETNKAKAFMYH+FAAEGNKQSKMALAY YF
Sbjct: 121 VADIDQAVAESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHHFAAEGNKQSKMALAYIYF 180

Query: 216 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQ 275
           RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQ
Sbjct: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQ 240

Query: 276 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYAR 335
           ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKAL WFSKAV+KGEPKSMELLGEIYAR
Sbjct: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR 300

Query: 336 GXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 395
           GXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 301 GXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 360

Query: 396 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 455
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 456 XXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 515
           XXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ
Sbjct: 421 XXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480

Query: 516 SMCLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 575
           SMCLGESGFCTDAE HQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM
Sbjct: 481 SMCLGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 540

Query: 576 HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRK 635
           HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLAL SLWLR 
Sbjct: 541 HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRM 600

Query: 636 NHADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLREXXXXXXXX 695
           NHADSFLVHVIDSLPEVYPKID WVEDVLLEEGNATILTLFACLLTVLYLRE        
Sbjct: 601 NHADSFLVHVIDSLPEVYPKIDAWVEDVLLEEGNATILTLFACLLTVLYLRE------RQ 660

Query: 696 XXXXXXXXXXAVPLHPNDHVAAQN 718
                     AVPLHPNDHV  QN
Sbjct: 661 RRHAAVRAAEAVPLHPNDHVPPQN 678

BLAST of Carg04850 vs. TrEMBL
Match: tr|A0A1S3CMW9|A0A1S3CMW9_CUCME (ERAD-associated E3 ubiquitin-protein ligase component HRD3A OS=Cucumis melo OX=3656 GN=LOC103502593 PE=4 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 1.5e-249
Identity = 621/684 (90.79%), Postives = 632/684 (92.40%), Query Frame = 0

Query: 36  MQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFG 95
           MQL+ RR+QLIFLI+CL S FINARPFLIVISQDDLKD A PDDSSDSAN DSA+WDEFG
Sbjct: 1   MQLETRRVQLIFLILCLSSLFINARPFLIVISQDDLKDGAPPDDSSDSANSDSADWDEFG 60

Query: 96  --EPESQNAAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDA 155
                                          DAP+DLYYTALGKMM+AVSSGDLRLMEDA
Sbjct: 61  XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDAPQDLYYTALGKMMSAVSSGDLRLMEDA 120

Query: 156 VGDIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAEGNKQSKMALAYSYF 215
           V DIDQ   ++GDPHAQSVLGLLYGMGIMKETNKAKAFMYH+FAAEGNKQSKMALAY YF
Sbjct: 121 VADIDQAVADNGDPHAQSVLGLLYGMGIMKETNKAKAFMYHHFAAEGNKQSKMALAYIYF 180

Query: 216 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQ 275
           RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQ
Sbjct: 181 RQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQ 240

Query: 276 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYAR 335
           ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKAL WFSKAV+KGEPKSMELLGEIYAR
Sbjct: 241 ILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALSWFSKAVEKGEPKSMELLGEIYAR 300

Query: 336 GXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 395
           GXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 301 GXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 360

Query: 396 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 455
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 456 XXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 515
           XXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ
Sbjct: 421 XXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQ 480

Query: 516 SMCLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 575
           SMCLGESGFCTDAE HQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM
Sbjct: 481 SMCLGESGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYM 540

Query: 576 HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRK 635
           HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLAL SLWLRK
Sbjct: 541 HAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALVSLWLRK 600

Query: 636 NHADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLREXXXXXXXX 695
           NHADSFLVHVIDSLPEVYPKID WVEDVLLEEGNATILTLFACLLTVLYLRE  XXXXXX
Sbjct: 601 NHADSFLVHVIDSLPEVYPKIDAWVEDVLLEEGNATILTLFACLLTVLYLRE-RXXXXXX 660

Query: 696 XXXXXXXXXXAVPLHPNDHVAAQN 718
           XXXXXXXXXX  PLHPNDH+  QN
Sbjct: 661 XXXXXXXXXXVPPLHPNDHLPPQN 683

BLAST of Carg04850 vs. TrEMBL
Match: tr|A0A1Q3CLW9|A0A1Q3CLW9_CEPFO (Sel1 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_24552 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 3.8e-205
Identity = 469/646 (72.60%), Postives = 526/646 (81.42%), Query Frame = 0

Query: 42  RLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEFGEPESQN 101
           RL L  LI+CL    + ARP +  ++ DDLKD A+   + D + +D+ EWD+FG      
Sbjct: 2   RLLLPLLILCLHPLSLPARPVIFYLTHDDLKDVATDAPAEDDSLHDTPEWDDFGGDSDSK 61

Query: 102 AAQELDPGTWRPIFEPDSPASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDAVGDIDQVA 161
             ++LDPG+WRPIFEP    + P      Y++A+ K+++A S GDLRLME+A  +I++ A
Sbjct: 62  PEEDLDPGSWRPIFEPAGGLASP------YHSAVAKILSAASGGDLRLMEEAAEEIEE-A 121

Query: 162 VESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAE-GNKQSKMALAYSYFRQEMYEK 221
              G+PH QSVLG LY MG+ KE NK KA +YHYF+A+ G+ QSK+ALAY+Y RQEMYEK
Sbjct: 122 AGRGNPHTQSVLGFLYAMGMTKERNKGKALLYHYFSADGGSTQSKLALAYTYLRQEMYEK 181

Query: 222 AVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDFQILEYQAQ 281
           AV LYAELAEVA++S L+SKDSPVIEP+RIHNGAEENK+ALRKSRGEEDEDFQILEYQAQ
Sbjct: 182 AVILYAELAEVAVSSFLISKDSPVIEPIRIHNGAEENKEALRKSRGEEDEDFQILEYQAQ 241

Query: 282 KGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYARG-XXXXX 341
           KGNAGAMY++GLFYYFGLRGLRRDHAKAL WFSKAV+KGEP+SMELLGEIYARG      
Sbjct: 242 KGNAGAMYKVGLFYYFGLRGLRRDHAKALWWFSKAVEKGEPRSMELLGEIYARGAGVERN 301

Query: 342 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 401
                                             XXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 302 YTRALECFTLASRQQLYSAYNGMGFLYVKGYGVEXXXXXXXXXXXXXXXXXXXXXXXXXX 361

Query: 402 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 461
           XXXXXXXXXXXX      XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 362 XXXXXXXXXXXXDVKLACXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 421

Query: 462 XXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYGEQSMCLGE 521
           XXXXPWSSLSRWALESYLK D+GKAF LYARMAELG+EVAQSNAAWILDKYGE SMC+GE
Sbjct: 422 XXXXPWSSLSRWALESYLKGDVGKAFLLYARMAELGFEVAQSNAAWILDKYGESSMCMGE 481

Query: 522 SGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEAYMHAKSQL 581
           SGFCTDAE HQRAHSLWWQASEQGNEHAALLIGDAYYYGRGT+ DY RAAEAY+HAKSQ 
Sbjct: 482 SGFCTDAERHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTERDYYRAAEAYIHAKSQS 541

Query: 582 NAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWLRKNHADSF 641
           NAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQA E+DPAA+LPV LAL SLW+RKNH DSF
Sbjct: 542 NAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQAQEIDPAAKLPVTLALTSLWIRKNHPDSF 601

Query: 642 LVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRE 686
           +VH+IDSLPEVYPK++ WVE+V++EEGNATILTLF CLLTVLYLRE
Sbjct: 602 MVHLIDSLPEVYPKVEAWVENVIMEEGNATILTLFVCLLTVLYLRE 640

BLAST of Carg04850 vs. TrEMBL
Match: tr|A0A175YDV1|A0A175YDV1_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_029422 PE=4 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 8.6e-205
Identity = 514/654 (78.59%), Postives = 579/654 (88.53%), Query Frame = 0

Query: 35  RMQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDEF 94
           R++   + L ++ L++C+    I +RPF++V+SQDDLKD +  D  S      ++EWDEF
Sbjct: 4   RVKTHRKILTILTLVLCIYPVSIYSRPFVLVLSQDDLKDPSPSDPLSPEPADSNSEWDEF 63

Query: 95  GEPESQNAAQELDPGTWRPIFEPDS-PASDPDAPEDLYYTALGKMMTAVSSGDLRLMEDA 154
           G+ +S+ +  ELDPGTWRPIFEP+S P  DP+  ED YY+ + +MM AVS GD+R+ME+ 
Sbjct: 64  GDSDSK-SDDELDPGTWRPIFEPESDPTRDPNW-EDGYYSGVRRMMGAVSRGDVRMMEEG 123

Query: 155 VGDIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAE-GNKQSKMALAYSY 214
           VG+I++ A   G  H QS++G LY MG+++E +KAK FMYHYFAAE GN QSKMALAY+Y
Sbjct: 124 VGEIEE-AARGGHAHGQSMMGYLYNMGVLRERSKAKGFMYHYFAAEAGNMQSKMALAYTY 183

Query: 215 FRQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGEEDEDF 274
            RQ+M++KAVKLYAELAEVA+NS L+SKDSPVIEPVRIHNGAEENK+ALRKSRGEEDEDF
Sbjct: 184 TRQDMHDKAVKLYAELAEVAVNSFLISKDSPVIEPVRIHNGAEENKEALRKSRGEEDEDF 243

Query: 275 QILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELLGEIYA 334
           QILEYQAQKGNAGAMY+IG+FYYFGLRGLRRDH+KAL WF KAV+K EP+SMELLGEIYA
Sbjct: 244 QILEYQAQKGNAGAMYKIGIFYYFGLRGLRRDHSKALYWFLKAVEKEEPRSMELLGEIYA 303

Query: 335 RG-XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 394
           RG XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 304 RGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 363

Query: 395 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 454
           XXXXXXXXXXXXXXXXXXXX    XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 364 XXXXXXXXXXXXXXXXXXXXDVKVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 423

Query: 455 XXXXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWILDKYG 514
           XXXXXXXXXXXXPWSSLSRW+LE+YLK D+GKAF LY+RMAELGYEVAQSNAAWILDKYG
Sbjct: 424 XXXXXXXXXXXXPWSSLSRWSLEAYLKGDVGKAFLLYSRMAELGYEVAQSNAAWILDKYG 483

Query: 515 EQSMCLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYDRAAEA 574
           E+SMC+GESGFCTDAE HQ AHSLWWQASEQGNEHAALLIGDAYYYGRGT+ DYDRAAEA
Sbjct: 484 ERSMCMGESGFCTDAERHQIAHSLWWQASEQGNEHAALLIGDAYYYGRGTERDYDRAAEA 543

Query: 575 YMHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLALASLWL 634
           YMHAKSQ NAQAMFNLGYMHEHG GLPFDLHLAKRYYDQA+E+DP+A+LPV LAL SLW+
Sbjct: 544 YMHAKSQSNAQAMFNLGYMHEHGQGLPFDLHLAKRYYDQAVEIDPSAKLPVTLALGSLWV 603

Query: 635 RKNHADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRE 686
           RKN ADSFLV  IDSLP V+PK+ +WVE+V+LEEGNATILTLF CLLTVLYLRE
Sbjct: 604 RKNCADSFLVDFIDSLPYVFPKVQLWVENVVLEEGNATILTLFVCLLTVLYLRE 654

BLAST of Carg04850 vs. TrEMBL
Match: tr|A0A1J7GVR9|A0A1J7GVR9_LUPAN (Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_13801 PE=4 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 3.3e-204
Identity = 513/659 (77.85%), Postives = 578/659 (87.71%), Query Frame = 0

Query: 34  IRMQLQARRLQLIFLIICLCSFFINARPFLIVISQDDLKDAASPDDSSDSANYDSAEWDE 93
           +R +    +  L  LI  L    + ARPF++V+SQ++ KD   P D + + + +SAEWD+
Sbjct: 1   MRRRTPTTKFILSLLIFSLLPLSLLARPFVLVLSQEEFKDV--PSDEAPAVDPESAEWDD 60

Query: 94  FGEPESQNAAQELDPGTWRPIFEPDSPASDPDAP-----EDLYYTALGKMMTAVSSGDLR 153
           FG+ ++  + +ELDPG+WR IFEPDS  S   A      E LYY+ +GKMM+AV+S ++R
Sbjct: 61  FGDSDAGKSEEELDPGSWRQIFEPDSTFSTTTAEPSTELEALYYSGVGKMMSAVTSAEVR 120

Query: 154 LMEDAVGDIDQVAVESGDPHAQSVLGLLYGMGIMKETNKAKAFMYHYFAAE-GNKQSKMA 213
           LME+A G+I + A ESG P AQSVLG L+G+G+++E +K+KAF+YH+FA+E GN QSKMA
Sbjct: 121 LMEEAAGEI-EAAAESGYPAAQSVLGFLWGIGLLRERSKSKAFLYHHFASEGGNMQSKMA 180

Query: 214 LAYSYFRQEMYEKAVKLYAELAEVAINSLLVSKDSPVIEPVRIHNGAEENKQALRKSRGE 273
           LAY+Y RQ+M+EKAVKLYAELAEVA+NS L+SKDSPVIEPVR+HNGAEENK+ALRKS+GE
Sbjct: 181 LAYTYTRQDMFEKAVKLYAELAEVAVNSFLISKDSPVIEPVRLHNGAEENKEALRKSKGE 240

Query: 274 EDEDFQILEYQAQKGNAGAMYRIGLFYYFGLRGLRRDHAKALIWFSKAVDKGEPKSMELL 333
           EDEDFQILEYQAQKGNAGAMY++GLFYYFGLRGLRRDH KAL WF KAV+KGEP+SMELL
Sbjct: 241 EDEDFQILEYQAQKGNAGAMYKVGLFYYFGLRGLRRDHTKALSWFLKAVEKGEPRSMELL 300

Query: 334 GEIYARG-XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 393
           GEIYARG XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXX
Sbjct: 301 GEIYARGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKXXXXXXXXXXXX 360

Query: 394 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 453
           XXXXXXXXXXXXXXXXXXXXXXXXX     XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXDVKLAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 454 XXXXXXXXXXXXXXXXXPWSSLSRWALESYLKSDIGKAFFLYARMAELGYEVAQSNAAWI 513
           XXXXXXXXXXXXXXXXX WSSLSRWALESYLK D+GKAF LY+RMAE+GYEVAQSNAAWI
Sbjct: 421 XXXXXXXXXXXXXXXXXXWSSLSRWALESYLKGDVGKAFMLYSRMAEMGYEVAQSNAAWI 480

Query: 514 LDKYGEQSMCLGESGFCTDAELHQRAHSLWWQASEQGNEHAALLIGDAYYYGRGTDVDYD 573
           LDKYGE+SMC+GESGFCTDAE HQ AHSLWWQASEQGNEHAALLIGDAYYYGRGT  DY+
Sbjct: 481 LDKYGERSMCMGESGFCTDAERHQHAHSLWWQASEQGNEHAALLIGDAYYYGRGTIRDYE 540

Query: 574 RAAEAYMHAKSQLNAQAMFNLGYMHEHGLGLPFDLHLAKRYYDQALELDPAARLPVKLAL 633
           RAAEAYMHAKSQ NAQAMFNLGYMHEHG GLPFDLHLAKRYYD+AL+ DPAA+LPV LAL
Sbjct: 541 RAAEAYMHAKSQSNAQAMFNLGYMHEHGQGLPFDLHLAKRYYDEALQHDPAAKLPVTLAL 600

Query: 634 ASLWLRKNHADSFLVHVIDSLPEVYPKIDVWVEDVLLEEGNATILTLFACLLTVLYLRE 686
           ASLW+RKN+  S LVHVIDSLPEVYPK++ WVE+VLLEEGNATILTLFACLLTVLYLRE
Sbjct: 601 ASLWVRKNYDGSLLVHVIDSLPEVYPKLEAWVEEVLLEEGNATILTLFACLLTVLYLRE 656

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023001775.15.7e-29399.42ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita maxi... [more]
XP_023519772.11.8e-29199.71ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita pepo... [more]
XP_022924200.19.0e-29197.23ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita mosc... [more]
XP_022994230.11.1e-26491.36ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita maxi... [more]
XP_023542435.18.8e-26290.92ERAD-associated E3 ubiquitin-protein ligase component HRD3A-like [Cucurbita pepo... [more]
Match NameE-valueIdentityDescription
AT1G18260.19.0e-19275.12HCP-like superfamily protein[more]
AT1G73570.11.2e-16461.31HCP-like superfamily protein[more]
Match NameE-valueIdentityDescription
sp|Q9LM25|HRD3A_ARATH1.6e-19075.12ERAD-associated E3 ubiquitin-protein ligase component HRD3A OS=Arabidopsis thali... [more]
sp|Q9C6B6|HRD3B_ARATH2.2e-16361.31ERAD-associated E3 ubiquitin-protein ligase component HRD3B OS=Arabidopsis thali... [more]
sp|Q10NT7|HRD3_ORYSJ3.5e-15367.30ERAD-associated E3 ubiquitin-protein ligase component HRD3 OS=Oryza sativa subsp... [more]
sp|Q9ESM7|SE1L1_MESAU4.5e-3140.63Protein sel-1 homolog 1 OS=Mesocricetus auratus OX=10036 GN=Sel1l PE=2 SV=1[more]
sp|Q9UBV2|SE1L1_HUMAN5.8e-3140.63Protein sel-1 homolog 1 OS=Homo sapiens OX=9606 GN=SEL1L PE=1 SV=3[more]
Match NameE-valueIdentityDescription
tr|A0A0A0LQX2|A0A0A0LQX2_CUCSA6.1e-25189.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G042850 PE=4 SV=1[more]
tr|A0A1S3CMW9|A0A1S3CMW9_CUCME1.5e-24990.79ERAD-associated E3 ubiquitin-protein ligase component HRD3A OS=Cucumis melo OX=3... [more]
tr|A0A1Q3CLW9|A0A1Q3CLW9_CEPFO3.8e-20572.60Sel1 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_245... [more]
tr|A0A175YDV1|A0A175YDV1_DAUCA8.6e-20578.59Uncharacterized protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_029422 ... [more]
tr|A0A1J7GVR9|A0A1J7GVR9_LUPAN3.3e-20477.85Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_13801 PE=4 S... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
Vocabulary: INTERPRO
TermDefinition
IPR011990TPR-like_helical_dom_sf
IPR006597Sel1-like
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04850-RACarg04850-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006597Sel1-like repeatSMARTSM00671sel1coord: 321..356
e-value: 5.5E-6
score: 35.9
coord: 581..616
e-value: 8.9E-8
score: 41.8
coord: 357..392
e-value: 2.0E-5
score: 34.0
coord: 167..202
e-value: 2.9
score: 16.9
coord: 284..320
e-value: 5.2E-8
score: 42.6
coord: 393..428
e-value: 6.5E-7
score: 39.0
coord: 429..464
e-value: 0.0064
score: 25.7
coord: 545..580
e-value: 9.8
score: 13.7
IPR006597Sel1-like repeatPFAMPF08238Sel1coord: 581..615
e-value: 1.1E-5
score: 25.8
coord: 358..391
e-value: 4.4E-6
score: 27.1
coord: 546..569
e-value: 4.3
score: 8.1
coord: 285..319
e-value: 8.0E-8
score: 32.6
coord: 322..355
e-value: 2.2E-6
score: 28.0
coord: 396..427
e-value: 3.2E-4
score: 21.2
coord: 167..200
e-value: 0.71
score: 10.5
coord: 433..463
e-value: 34.0
score: 5.2
coord: 478..496
e-value: 32.0
score: 5.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3DG3DSA:1.25.40.10coord: 151..242
e-value: 1.5E-7
score: 32.8
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3DG3DSA:1.25.40.10coord: 262..639
e-value: 7.4E-84
score: 283.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..126
NoneNo IPR availablePANTHERPTHR45084FAMILY NOT NAMEDcoord: 37..702
NoneNo IPR availableSUPERFAMILYSSF81901HCP-likecoord: 265..391
NoneNo IPR availableSUPERFAMILYSSF81901HCP-likecoord: 476..617
NoneNo IPR availableSUPERFAMILYSSF81901HCP-likecoord: 160..230
NoneNo IPR availableSUPERFAMILYSSF81901HCP-likecoord: 370..504