Carg03188 (gene) Silver-seed gourd

NameCarg03188
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionPhospholipid:diacylglycerol acyltransferase
LocationCucurbita_argyrosperma_scaffold_005 : 534084 .. 535054 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCAGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGACGGATGGACTGAATATGATGAGATGAGTAGGGAAAGTCTTCGGAAAGTCTCCGAGAATAAAGCTTACACAGCAAAAACTGTTTTTGATCTACTCCGAGTCGTGGCTCCAAAAATGATGCAGAGGATGGATAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCAAAATATGCTCATTACAAGTACTGGTCAAATCCACTAGAGACAAAGTAAGTAGCTGTGTAGTTTTTTAGAATTTTCTGATTTCTTTAGAAGATAATCTTCCACATGAAGTACTGAATTGAATCAACGCTTGGCATATGAGCTGATAATCTATCTAATAACTATCTAACATCAAGAACATGGTTTTGGAGATGGTATTATTAGCTAATTTCAAATACAATCAAACAACTTCCCTCAATCCCATGAGACTTGATACATTTTGCAAAATTTGTTTCAGACTACCAGATGCCCCAGATATGGAGATATACTGCTTATACGGAGTGGGAATCCCCACAGAAAGATCATATATCTATGACTCATCTTATGATAAGTGCAAGAGCATTCCATTCAGGATTGATCTCTCAATCCATGGAGAAGGGAGTGGCTGTCTAAGAAGTGGGATGTATTTTGTCGATGGCGACGACAGTGTACCAGTGGTGAGCTCTGGTTTCATGTGTGCGAAAGGGTGGCGAAACAAAACACGATTCAATCCATCAGGCAGCCCTACATACGTAAGAGAATACAGGCACAAGGCTCCTGCTAGTCTGCTAGAAGGAAGAGGAATTGAAAGTAGTGCTCACGTTGACATAATGGGCAATGCTAAACTAATTGAAGATATCTTGAGGGTTGCTGCTGGAGCCACTGGAGCAGAGATTGGTGGTGATAGGATTTACTCAGATATATTGACACTATCAGAGAGAATAAATCTTAAGCTGTGA

mRNA sequence

ATGGATTCAGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGACGGATGGACTGAATATGATGAGATGAGTAGGGAAAGTCTTCGGAAAGTCTCCGAGAATAAAGCTTACACAGCAAAAACTGTTTTTGATCTACTCCGAGTCGTGGCTCCAAAAATGATGCAGAGGATGGATAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCAAAATATGCTCATTACAAGTACTGGTCAAATCCACTAGAGACAAAACTACCAGATGCCCCAGATATGGAGATATACTGCTTATACGGAGTGGGAATCCCCACAGAAAGATCATATATCTATGACTCATCTTATGATAAGTGCAAGAGCATTCCATTCAGGATTGATCTCTCAATCCATGGAGAAGGGAGTGGCTGTCTAAGAAGTGGGATGTATTTTGTCGATGGCGACGACAGTGTACCAGTGGTGAGCTCTGGTTTCATGTGTGCGAAAGGGTGGCGAAACAAAACACGATTCAATCCATCAGGCAGCCCTACATACGTAAGAGAATACAGGCACAAGGCTCCTGCTAGTCTGCTAGAAGGAAGAGGAATTGAAAGTAGTGCTCACGTTGACATAATGGGCAATGCTAAACTAATTGAAGATATCTTGAGGGTTGCTGCTGGAGCCACTGGAGCAGAGATTGGTGGTGATAGGATTTACTCAGATATATTGACACTATCAGAGAGAATAAATCTTAAGCTGTGA

Coding sequence (CDS)

ATGGATTCAGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGACGGATGGACTGAATATGATGAGATGAGTAGGGAAAGTCTTCGGAAAGTCTCCGAGAATAAAGCTTACACAGCAAAAACTGTTTTTGATCTACTCCGAGTCGTGGCTCCAAAAATGATGCAGAGGATGGATAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCAAAATATGCTCATTACAAGTACTGGTCAAATCCACTAGAGACAAAACTACCAGATGCCCCAGATATGGAGATATACTGCTTATACGGAGTGGGAATCCCCACAGAAAGATCATATATCTATGACTCATCTTATGATAAGTGCAAGAGCATTCCATTCAGGATTGATCTCTCAATCCATGGAGAAGGGAGTGGCTGTCTAAGAAGTGGGATGTATTTTGTCGATGGCGACGACAGTGTACCAGTGGTGAGCTCTGGTTTCATGTGTGCGAAAGGGTGGCGAAACAAAACACGATTCAATCCATCAGGCAGCCCTACATACGTAAGAGAATACAGGCACAAGGCTCCTGCTAGTCTGCTAGAAGGAAGAGGAATTGAAAGTAGTGCTCACGTTGACATAATGGGCAATGCTAAACTAATTGAAGATATCTTGAGGGTTGCTGCTGGAGCCACTGGAGCAGAGATTGGTGGTGATAGGATTTACTCAGATATATTGACACTATCAGAGAGAATAAATCTTAAGCTGTGA

Protein sequence

MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSERINLKL
BLAST of Carg03188 vs. NCBI nr
Match: XP_023512597.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 501.1 bits (1289), Expect = 2.1e-138
Identity = 244/246 (99.19%), Postives = 245/246 (99.59%), Query Frame = 0

Query: 1   MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 60
           MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH
Sbjct: 449 MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 508

Query: 61  FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 120
           FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK
Sbjct: 509 FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 568

Query: 121 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 180
           SIPFRIDLSI GEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR
Sbjct: 569 SIPFRIDLSIDGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 628

Query: 181 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 240
           EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGG+RIYSDILTLSE
Sbjct: 629 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGERIYSDILTLSE 688

Query: 241 RINLKL 247
           RINLKL
Sbjct: 689 RINLKL 694

BLAST of Carg03188 vs. NCBI nr
Match: XP_022986276.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita maxima])

HSP 1 Score: 500.4 bits (1287), Expect = 3.6e-138
Identity = 243/246 (98.78%), Postives = 244/246 (99.19%), Query Frame = 0

Query: 1   MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 60
           MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH
Sbjct: 449 MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 508

Query: 61  FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 120
           FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK
Sbjct: 509 FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 568

Query: 121 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 180
           SIPFRIDLS+ GEGSGCLRSGMYFVDGDD VPVVSSGFMCAKGWRNKTRFNPSGSPTYVR
Sbjct: 569 SIPFRIDLSVDGEGSGCLRSGMYFVDGDDCVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 628

Query: 181 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 240
           EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE
Sbjct: 629 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 688

Query: 241 RINLKL 247
           RINLKL
Sbjct: 689 RINLKL 694

BLAST of Carg03188 vs. NCBI nr
Match: XP_022944139.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita moschata])

HSP 1 Score: 498.0 bits (1281), Expect = 1.8e-137
Identity = 242/246 (98.37%), Postives = 243/246 (98.78%), Query Frame = 0

Query: 1   MDSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 60
           MDSGSSND+SFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH
Sbjct: 449 MDSGSSNDTSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSH 508

Query: 61  FSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 120
           FSHGIAEDLDDPKY HYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK
Sbjct: 509 FSHGIAEDLDDPKYGHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCK 568

Query: 121 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 180
           SIPFRIDLSI GEGSGCLRSGMYFVDGDD VPVVSSGFMCAKGWRNKTRFNPSGSPTYVR
Sbjct: 569 SIPFRIDLSIDGEGSGCLRSGMYFVDGDDCVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 628

Query: 181 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 240
           EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE
Sbjct: 629 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 688

Query: 241 RINLKL 247
           RINLKL
Sbjct: 689 RINLKL 694

BLAST of Carg03188 vs. NCBI nr
Match: XP_008458650.1 (PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis melo])

HSP 1 Score: 466.5 bits (1199), Expect = 5.7e-128
Identity = 226/245 (92.24%), Postives = 235/245 (95.92%), Query Frame = 0

Query: 2   DSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHF 61
           +SG SNDSSFSCSD WTEYDEMSR+S+RKVSENKAYT KTVFDLLRVVAPKMMQRMDSHF
Sbjct: 562 NSGGSNDSSFSCSDVWTEYDEMSRDSIRKVSENKAYTVKTVFDLLRVVAPKMMQRMDSHF 621

Query: 62  SHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS 121
           SHGIAEDLDDPKYAH+KYWSNPLETKLPDAP+MEIYCLYGVGIPTERSYIYDSSYDKCKS
Sbjct: 622 SHGIAEDLDDPKYAHHKYWSNPLETKLPDAPNMEIYCLYGVGIPTERSYIYDSSYDKCKS 681

Query: 122 IPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVRE 181
           IPFRIDLSI G+GSGCLRSGMYFVDGDDSVP+VSSGFMCAKGWRNKTRFNPSGSPTYVRE
Sbjct: 682 IPFRIDLSIEGKGSGCLRSGMYFVDGDDSVPIVSSGFMCAKGWRNKTRFNPSGSPTYVRE 741

Query: 182 YRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSER 241
           YRHKAPASLLEGRG+ESSAHVDIMGN  LIEDILRVAA   G EIGGD+IYSDILTLSER
Sbjct: 742 YRHKAPASLLEGRGVESSAHVDIMGNVNLIEDILRVAA---GEEIGGDKIYSDILTLSER 801

Query: 242 INLKL 247
           INLKL
Sbjct: 802 INLKL 803

BLAST of Carg03188 vs. NCBI nr
Match: XP_004145827.1 (PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis sativus] >KGN46906.1 hypothetical protein Csa_6G149960 [Cucumis sativus])

HSP 1 Score: 466.1 bits (1198), Expect = 7.5e-128
Identity = 226/245 (92.24%), Postives = 235/245 (95.92%), Query Frame = 0

Query: 2   DSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHF 61
           +SG SNDSSFSCSD WTEYDEMSRES+RKVSENKAYT +TVFDLLR+VAPKMMQRMDSHF
Sbjct: 449 NSGGSNDSSFSCSDVWTEYDEMSRESIRKVSENKAYTVETVFDLLRIVAPKMMQRMDSHF 508

Query: 62  SHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS 121
           SHGIAEDLDDPKYAH+KYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS
Sbjct: 509 SHGIAEDLDDPKYAHHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS 568

Query: 122 IPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVRE 181
           IPFRIDLSI G+GSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPT+VRE
Sbjct: 569 IPFRIDLSIEGKGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTHVRE 628

Query: 182 YRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSER 241
           YRHKAPASLLEGRG+ESSAHVDIMGN  LIEDILRVAA   G EIGGD+IYSDILTLSER
Sbjct: 629 YRHKAPASLLEGRGVESSAHVDIMGNVNLIEDILRVAA---GEEIGGDKIYSDILTLSER 688

Query: 242 INLKL 247
           INLKL
Sbjct: 689 INLKL 690

BLAST of Carg03188 vs. TAIR10
Match: AT3G44830.1 (Lecithin:cholesterol acyltransferase family protein)

HSP 1 Score: 364.4 bits (934), Expect = 5.6e-101
Identity = 176/246 (71.54%), Postives = 209/246 (84.96%), Query Frame = 0

Query: 4   GSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSH 63
           G+SNDS+ SC + W+EY+EMSRES+ KV+EN AYTA TV DLLR +APKMM+R ++HFSH
Sbjct: 422 GNSNDST-SCGEFWSEYNEMSRESIVKVAENTAYTATTVLDLLRFIAPKMMRRAEAHFSH 481

Query: 64  GIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYD--SSYDKCK- 123
           GIA+DLDDPKY HYKYWSNPLETKLP+AP+ME+YCLYGVGIPTERSYIY   +S  KCK 
Sbjct: 482 GIADDLDDPKYGHYKYWSNPLETKLPEAPEMEMYCLYGVGIPTERSYIYKLATSSGKCKS 541

Query: 124 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 183
           SIPFRID S+ G+   CL+ G  F DGD+SVPV+S+GFMCAKGWR KTRFNPSG  T++R
Sbjct: 542 SIPFRIDGSLDGD-DVCLKGGTRFADGDESVPVISAGFMCAKGWRGKTRFNPSGMDTFLR 601

Query: 184 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 243
           EY+HK P SLLE RG ES AHVDIMGN  LIED+LR+AAGA+G EIGGDRIYSD++ +SE
Sbjct: 602 EYKHKPPGSLLESRGTESGAHVDIMGNVGLIEDVLRIAAGASGQEIGGDRIYSDVMRMSE 661

Query: 244 RINLKL 247
           RI++KL
Sbjct: 662 RISIKL 665

BLAST of Carg03188 vs. TAIR10
Match: AT5G13640.1 (phospholipid:diacylglycerol acyltransferase)

HSP 1 Score: 301.2 bits (770), Expect = 5.8e-82
Identity = 144/236 (61.02%), Postives = 177/236 (75.00%), Query Frame = 0

Query: 12  SCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDD 71
           +C D WTEY +M    ++ ++E K YTA    DLL  VAPKMM R  +HFS+GIA+DLDD
Sbjct: 436 TCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLLHYVAPKMMARGAAHFSYGIADDLDD 495

Query: 72  PKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIH 131
            KY   KYWSNPLETKLP+AP+MEIY LYGVGIPTER+Y+Y  +      IPF+I  S H
Sbjct: 496 TKYQDPKYWSNPLETKLPNAPEMEIYSLYGVGIPTERAYVYKLNQSPDSCIPFQIFTSAH 555

Query: 132 GEG-SGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASL 191
            E    CL++G+Y VDGD++VPV+S+G+MCAK WR KTRFNPSG  TY+REY H  PA+L
Sbjct: 556 EEDEDSCLKAGVYNVDGDETVPVLSAGYMCAKAWRGKTRFNPSGIKTYIREYNHSPPANL 615

Query: 192 LEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSERINLKL 247
           LEGRG +S AHVDIMGN  LIEDI+RVAAG  G++IG D+++S I   SERI+LKL
Sbjct: 616 LEGRGTQSGAHVDIMGNFALIEDIMRVAAGGNGSDIGHDQVHSGIFEWSERIDLKL 671

BLAST of Carg03188 vs. Swiss-Prot
Match: sp|Q9FYC7|PDAT2_ARATH (Putative phospholipid:diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PDAT2 PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.0e-99
Identity = 176/246 (71.54%), Postives = 209/246 (84.96%), Query Frame = 0

Query: 4   GSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSH 63
           G+SNDS+ SC + W+EY+EMSRES+ KV+EN AYTA TV DLLR +APKMM+R ++HFSH
Sbjct: 422 GNSNDST-SCGEFWSEYNEMSRESIVKVAENTAYTATTVLDLLRFIAPKMMRRAEAHFSH 481

Query: 64  GIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYD--SSYDKCK- 123
           GIA+DLDDPKY HYKYWSNPLETKLP+AP+ME+YCLYGVGIPTERSYIY   +S  KCK 
Sbjct: 482 GIADDLDDPKYGHYKYWSNPLETKLPEAPEMEMYCLYGVGIPTERSYIYKLATSSGKCKS 541

Query: 124 SIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVR 183
           SIPFRID S+ G+   CL+ G  F DGD+SVPV+S+GFMCAKGWR KTRFNPSG  T++R
Sbjct: 542 SIPFRIDGSLDGD-DVCLKGGTRFADGDESVPVISAGFMCAKGWRGKTRFNPSGMDTFLR 601

Query: 184 EYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSE 243
           EY+HK P SLLE RG ES AHVDIMGN  LIED+LR+AAGA+G EIGGDRIYSD++ +SE
Sbjct: 602 EYKHKPPGSLLESRGTESGAHVDIMGNVGLIEDVLRIAAGASGQEIGGDRIYSDVMRMSE 661

Query: 244 RINLKL 247
           RI++KL
Sbjct: 662 RISIKL 665

BLAST of Carg03188 vs. Swiss-Prot
Match: sp|Q9FNA9|PDAT1_ARATH (Phospholipid:diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PDAT1 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.0e-80
Identity = 144/236 (61.02%), Postives = 177/236 (75.00%), Query Frame = 0

Query: 12  SCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDD 71
           +C D WTEY +M    ++ ++E K YTA    DLL  VAPKMM R  +HFS+GIA+DLDD
Sbjct: 436 TCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLLHYVAPKMMARGAAHFSYGIADDLDD 495

Query: 72  PKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIH 131
            KY   KYWSNPLETKLP+AP+MEIY LYGVGIPTER+Y+Y  +      IPF+I  S H
Sbjct: 496 TKYQDPKYWSNPLETKLPNAPEMEIYSLYGVGIPTERAYVYKLNQSPDSCIPFQIFTSAH 555

Query: 132 GEG-SGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASL 191
            E    CL++G+Y VDGD++VPV+S+G+MCAK WR KTRFNPSG  TY+REY H  PA+L
Sbjct: 556 EEDEDSCLKAGVYNVDGDETVPVLSAGYMCAKAWRGKTRFNPSGIKTYIREYNHSPPANL 615

Query: 192 LEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSERINLKL 247
           LEGRG +S AHVDIMGN  LIEDI+RVAAG  G++IG D+++S I   SERI+LKL
Sbjct: 616 LEGRGTQSGAHVDIMGNFALIEDIMRVAAGGNGSDIGHDQVHSGIFEWSERIDLKL 671

BLAST of Carg03188 vs. Swiss-Prot
Match: sp|P40345|PDAT_YEAST (Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=LRO1 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 4.0e-24
Identity = 69/219 (31.51%), Postives = 124/219 (56.62%), Query Frame = 0

Query: 5   SSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQ-RMDSHFSH 64
           SS D+  + +D +  +    R +    + NK  T K   ++   ++P+ +Q R+   +S 
Sbjct: 432 SSEDALNNNTDTYGNFIRFERNT--SDAFNKNLTMKDAINMTLSISPEWLQRRVHEQYSF 491

Query: 65  GIAEDLDD--PKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS 124
           G +++ ++      H+K+WSNP+E  LP+AP M+IYC+YGV  PTER+Y+Y    D    
Sbjct: 492 GYSKNEEELRKNELHHKHWSNPMEVPLPEAPHMKIYCIYGVNNPTERAYVYKEEDDSS-- 551

Query: 125 IPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVRE 184
               ++L+I  E     +  ++  +GD +VP+V+   MC K  +  + +NP+G    + E
Sbjct: 552 ---ALNLTIDYES----KQPVFLTEGDGTVPLVAHS-MCHKWAQGASPYNPAGINVTIVE 611

Query: 185 YRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAG 221
            +H+     + G G +S+ HVDI+G+A+L + IL++A+G
Sbjct: 612 MKHQPDRFDIRG-GAKSAEHVDILGSAELNDYILKIASG 637

BLAST of Carg03188 vs. Swiss-Prot
Match: sp|O94680|PDAT_SCHPO (Phospholipid:diacylglycerol acyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=plh1 PE=1 SV=2)

HSP 1 Score: 112.5 bits (280), Expect = 6.9e-24
Identity = 74/189 (39.15%), Postives = 114/189 (60.32%), Query Frame = 0

Query: 60  HFSHGIA----EDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSS 119
           ++SHG+A    E L + +      W NPLET LP APDM+IYC++GVG PTER Y Y ++
Sbjct: 451 NYSHGLAWTEKEVLKNNEMP--SKWINPLETSLPYAPDMKIYCVHGVGKPTERGYYYTNN 510

Query: 120 YDKCKSIPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGS 179
            +     P  ID S++ +G+  + +G+   DGD ++P+++ G +C K W+ K RFNP+ +
Sbjct: 511 PE---GQPV-IDSSVN-DGTK-VENGIVMDDGDGTLPILALGLVCNKVWQTK-RFNPANT 570

Query: 180 PTYVREYRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDI 239
                E +H+  A  L G G  S+ HVDI+G+++L E IL+V++G    +   +R  SDI
Sbjct: 571 SITNYEIKHEPAAFDLRG-GPRSAEHVDILGHSELNEIILKVSSG--HGDSVPNRYISDI 627

Query: 240 LTLSERINL 245
             +   INL
Sbjct: 631 QEIINEINL 627

BLAST of Carg03188 vs. Swiss-Prot
Match: sp|Q08758|LCAT_PAPAN (Phosphatidylcholine-sterol acyltransferase OS=Papio anubis OX=9555 GN=LCAT PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 42/141 (29.79%), Postives = 64/141 (45.39%), Query Frame = 0

Query: 81  SNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIHGEGSGCLRS 140
           S  L   LP AP +E+YCLYGVG+PT R+YIYD  +      P+   + +          
Sbjct: 321 SRDLLAGLP-APGVEVYCLYGVGLPTPRTYIYDHGF------PYTDPVDV---------- 380

Query: 141 GMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGIESSA 200
              + DGDD+V   S+  +C   W+                 R   P  LL  RGI+   
Sbjct: 381 --LYEDGDDTVATRSTE-LCGL-WQG----------------RQPQPVHLLPLRGIQ--- 421

Query: 201 HVDIMGNAKLIEDILRVAAGA 222
           H++++ + + +E I  +  GA
Sbjct: 441 HLNMVFSNQTLEHINAILLGA 421

BLAST of Carg03188 vs. TrEMBL
Match: tr|A0A1S3C9K8|A0A1S3C9K8_CUCME (putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo OX=3656 GN=LOC103497986 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 3.8e-128
Identity = 226/245 (92.24%), Postives = 235/245 (95.92%), Query Frame = 0

Query: 2   DSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHF 61
           +SG SNDSSFSCSD WTEYDEMSR+S+RKVSENKAYT KTVFDLLRVVAPKMMQRMDSHF
Sbjct: 562 NSGGSNDSSFSCSDVWTEYDEMSRDSIRKVSENKAYTVKTVFDLLRVVAPKMMQRMDSHF 621

Query: 62  SHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS 121
           SHGIAEDLDDPKYAH+KYWSNPLETKLPDAP+MEIYCLYGVGIPTERSYIYDSSYDKCKS
Sbjct: 622 SHGIAEDLDDPKYAHHKYWSNPLETKLPDAPNMEIYCLYGVGIPTERSYIYDSSYDKCKS 681

Query: 122 IPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVRE 181
           IPFRIDLSI G+GSGCLRSGMYFVDGDDSVP+VSSGFMCAKGWRNKTRFNPSGSPTYVRE
Sbjct: 682 IPFRIDLSIEGKGSGCLRSGMYFVDGDDSVPIVSSGFMCAKGWRNKTRFNPSGSPTYVRE 741

Query: 182 YRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSER 241
           YRHKAPASLLEGRG+ESSAHVDIMGN  LIEDILRVAA   G EIGGD+IYSDILTLSER
Sbjct: 742 YRHKAPASLLEGRGVESSAHVDIMGNVNLIEDILRVAA---GEEIGGDKIYSDILTLSER 801

Query: 242 INLKL 247
           INLKL
Sbjct: 802 INLKL 803

BLAST of Carg03188 vs. TrEMBL
Match: tr|A0A0A0KGM0|A0A0A0KGM0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149960 PE=4 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 5.0e-128
Identity = 226/245 (92.24%), Postives = 235/245 (95.92%), Query Frame = 0

Query: 2   DSGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHF 61
           +SG SNDSSFSCSD WTEYDEMSRES+RKVSENKAYT +TVFDLLR+VAPKMMQRMDSHF
Sbjct: 449 NSGGSNDSSFSCSDVWTEYDEMSRESIRKVSENKAYTVETVFDLLRIVAPKMMQRMDSHF 508

Query: 62  SHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS 121
           SHGIAEDLDDPKYAH+KYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS
Sbjct: 509 SHGIAEDLDDPKYAHHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKS 568

Query: 122 IPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVRE 181
           IPFRIDLSI G+GSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPT+VRE
Sbjct: 569 IPFRIDLSIEGKGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTHVRE 628

Query: 182 YRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSER 241
           YRHKAPASLLEGRG+ESSAHVDIMGN  LIEDILRVAA   G EIGGD+IYSDILTLSER
Sbjct: 629 YRHKAPASLLEGRGVESSAHVDIMGNVNLIEDILRVAA---GEEIGGDKIYSDILTLSER 688

Query: 242 INLKL 247
           INLKL
Sbjct: 689 INLKL 690

BLAST of Carg03188 vs. TrEMBL
Match: tr|A0A200R9P8|A0A200R9P8_9MAGN (Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferase OS=Macleaya cordata OX=56857 GN=BVC80_9057g15 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 2.2e-107
Identity = 186/239 (77.82%), Postives = 213/239 (89.12%), Query Frame = 0

Query: 9   SSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIAED 68
           S+ SC D WTEYDEMSRES+R ++ENKAYTA+T+ DLL  VAPKMM+R ++HFSHGIA++
Sbjct: 456 SNSSCGDVWTEYDEMSRESIRAIAENKAYTARTLTDLLHFVAPKMMRRTEAHFSHGIADN 515

Query: 69  LDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYD-SSYDKCKSIPFRID 128
           LDDPKY+HYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSY+Y  S  D+CKSIPFRID
Sbjct: 516 LDDPKYSHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYVYRLSPSDRCKSIPFRID 575

Query: 129 LSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAP 188
            S  G   GCLRSG+YFVDGD++VPV+S+GFMCAKGWR K RFNPSGSPTY+REY+HKAP
Sbjct: 576 SSADGSDGGCLRSGVYFVDGDETVPVLSAGFMCAKGWRGKNRFNPSGSPTYIREYKHKAP 635

Query: 189 ASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSERINLKL 247
           A LLEGRG+ES AHVDIMGN  LIED++RVAAGATG E+GGDRIYSDIL +SERINL+L
Sbjct: 636 A-LLEGRGLESGAHVDIMGNVALIEDVMRVAAGATGTEVGGDRIYSDILRMSERINLRL 693

BLAST of Carg03188 vs. TrEMBL
Match: tr|A0A2I4FNF8|A0A2I4FNF8_9ROSI (putative phospholipid:diacylglycerol acyltransferase 2 isoform X1 OS=Juglans regia OX=51240 GN=LOC109000668 PE=4 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 4.8e-107
Identity = 184/245 (75.10%), Postives = 218/245 (88.98%), Query Frame = 0

Query: 3   SGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFS 62
           + +S + + SC + WTEYDEMSRE +RK++ENKAYTA+T+FDLLR VAPKMMQR ++HFS
Sbjct: 444 TNTSTNFNSSCGEVWTEYDEMSRERIRKIAENKAYTARTLFDLLRSVAPKMMQRAEAHFS 503

Query: 63  HGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSS-YDKCKS 122
           HGI+E+LDDPKY HY+YWSNPLET+LPDAPDMEIYCLYGVGIPTER+Y+Y SS  +KCK+
Sbjct: 504 HGISENLDDPKYEHYRYWSNPLETRLPDAPDMEIYCLYGVGIPTERAYVYKSSPSEKCKN 563

Query: 123 IPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVRE 182
           IPFRID S  G+   CL+SG+YFVDGD+SVPV+S+GFMCAKGWR +TRFNPSG  T++RE
Sbjct: 564 IPFRIDSSAEGDEGSCLKSGVYFVDGDESVPVLSAGFMCAKGWRGRTRFNPSGMETHIRE 623

Query: 183 YRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSER 242
           YRHK PASLLEGRG+ES AHVDIMGN  LIEDILRVAAGATGAEIGGD+IYSDIL +SER
Sbjct: 624 YRHKPPASLLEGRGMESGAHVDIMGNVALIEDILRVAAGATGAEIGGDKIYSDILRMSER 683

Query: 243 INLKL 247
           IN++L
Sbjct: 684 INIRL 688

BLAST of Carg03188 vs. TrEMBL
Match: tr|A0A2I4FND1|A0A2I4FND1_9ROSI (putative phospholipid:diacylglycerol acyltransferase 2 isoform X2 OS=Juglans regia OX=51240 GN=LOC109000668 PE=4 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 4.8e-107
Identity = 184/245 (75.10%), Postives = 218/245 (88.98%), Query Frame = 0

Query: 3   SGSSNDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFS 62
           + +S + + SC + WTEYDEMSRE +RK++ENKAYTA+T+FDLLR VAPKMMQR ++HFS
Sbjct: 428 TNTSTNFNSSCGEVWTEYDEMSRERIRKIAENKAYTARTLFDLLRSVAPKMMQRAEAHFS 487

Query: 63  HGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSS-YDKCKS 122
           HGI+E+LDDPKY HY+YWSNPLET+LPDAPDMEIYCLYGVGIPTER+Y+Y SS  +KCK+
Sbjct: 488 HGISENLDDPKYEHYRYWSNPLETRLPDAPDMEIYCLYGVGIPTERAYVYKSSPSEKCKN 547

Query: 123 IPFRIDLSIHGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVRE 182
           IPFRID S  G+   CL+SG+YFVDGD+SVPV+S+GFMCAKGWR +TRFNPSG  T++RE
Sbjct: 548 IPFRIDSSAEGDEGSCLKSGVYFVDGDESVPVLSAGFMCAKGWRGRTRFNPSGMETHIRE 607

Query: 183 YRHKAPASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSER 242
           YRHK PASLLEGRG+ES AHVDIMGN  LIEDILRVAAGATGAEIGGD+IYSDIL +SER
Sbjct: 608 YRHKPPASLLEGRGMESGAHVDIMGNVALIEDILRVAAGATGAEIGGDKIYSDILRMSER 667

Query: 243 INLKL 247
           IN++L
Sbjct: 668 INIRL 672

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023512597.12.1e-13899.19putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita pepo subsp. pe... [more]
XP_022986276.13.6e-13898.78putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita maxima][more]
XP_022944139.11.8e-13798.37putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita moschata][more]
XP_008458650.15.7e-12892.24PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis melo][more]
XP_004145827.17.5e-12892.24PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis sativ... [more]
Match NameE-valueIdentityDescription
AT3G44830.15.6e-10171.54Lecithin:cholesterol acyltransferase family protein[more]
AT5G13640.15.8e-8261.02phospholipid:diacylglycerol acyltransferase[more]
Match NameE-valueIdentityDescription
sp|Q9FYC7|PDAT2_ARATH1.0e-9971.54Putative phospholipid:diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana O... [more]
sp|Q9FNA9|PDAT1_ARATH1.0e-8061.02Phospholipid:diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN... [more]
sp|P40345|PDAT_YEAST4.0e-2431.51Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ... [more]
sp|O94680|PDAT_SCHPO6.9e-2439.15Phospholipid:diacylglycerol acyltransferase OS=Schizosaccharomyces pombe (strain... [more]
sp|Q08758|LCAT_PAPAN1.1e-0529.79Phosphatidylcholine-sterol acyltransferase OS=Papio anubis OX=9555 GN=LCAT PE=2 ... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3C9K8|A0A1S3C9K8_CUCME3.8e-12892.24putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo OX=3656 G... [more]
tr|A0A0A0KGM0|A0A0A0KGM0_CUCSA5.0e-12892.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149960 PE=4 SV=1[more]
tr|A0A200R9P8|A0A200R9P8_9MAGN2.2e-10777.82Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferase OS=Macleaya cor... [more]
tr|A0A2I4FNF8|A0A2I4FNF8_9ROSI4.8e-10775.10putative phospholipid:diacylglycerol acyltransferase 2 isoform X1 OS=Juglans reg... [more]
tr|A0A2I4FND1|A0A2I4FND1_9ROSI4.8e-10775.10putative phospholipid:diacylglycerol acyltransferase 2 isoform X2 OS=Juglans reg... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008374O-acyltransferase activity
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR003386LACT/PDAT_acylTrfase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042967 acyl-carrier-protein biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0008374 O-acyltransferase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg03188-RACarg03188-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003386Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferasePFAMPF02450LCATcoord: 28..206
e-value: 2.1E-19
score: 69.9
NoneNo IPR availablePANTHERPTHR11440LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATEDcoord: 7..246
NoneNo IPR availablePANTHERPTHR11440:SF54PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE 2-RELATEDcoord: 7..246