Carg03169 (gene) Silver-seed gourd

NameCarg03169
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionjasmonic acid-amido synthetase JAR1
LocationCucurbita_argyrosperma_scaffold_005 : 439592 .. 443062 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGCTGCTGCTGCGGCTGCTGCGGCTGCGGGCGTGTTCAAGGCGGCGAGGTGCGCACCATGTTTGAAGAAGTAGAAGACATGGTTGATTGGTTCGAGCGAGTGTCGTCAGAAGCAGGCGTGGCTCAATTGCGCACACTCCGCGAGATTCTGCGCCAGAATTGTGGCGTGGAGTATCTAAACCAATGGCTTGGAGATGTCAATATCCATCAAATTCCTGATGACTTTGCATTGGAATCCCTCTTCACTTCCTTAATCCCCCTTTCTTCCCACGCTGATTTCGAGCCTTTTCTTCAACGAATCGCCGATGGTGACTCTTCCCCTTTGCTTACACAACAACCCATTCAAACTCTCTCTTTAAGGTATACCCTTCTTCAACATTTTCCCTTGCATTTTATGAATCCCTTCTTTATTACAAATGATATCATTACGCCGCTGCAGTTCTGGCACCACCGAAGGCCGACAAAAGTACGTGCCCTTCACTCCACATAGTGCGCAGACTACCCTTCTCATCTTTCGTATAGCTGCTGCTTATAGATCCAGGTACCAATGCAATATGGTCAGAATCTTCATTTTTTTTGAGTTACCCTTTTCTTGCAAGACATTAAAGAATGGCTTAATCACAACTAGGGTTTATCCCACAAGGGAAGGAGGCAGGATTTTGGAGTTCATTTACAGTAGTAAACAGACCAAAACCAAAGGAGGGATAACCACCGGCACAGCCACAACCCATTACTACGCCAGTGAGCAATTCAAAATCAAGCAGCTCAAAACCAAATCCTTCACTTGCAGCCCACAAGAAGTCATTTCCGGCGGTGATTACAAGCAATCCACTTACTGTCATCTTCTATTGGGGCTGTTCTTCTCCGACCAAGTCGAGTTTGTCACCTCAACTTTCGCTTACACCATAGTCCAAGCCTTCAACGAATTGGAAGATTGTTGGCCTCAAATCTGTCACGACATTACCCATGCCACACTCTGCTCTAGAATCACTCTTCCTGAAATCCGTAAGGCTGTTTTGAACATCATTAGTCCAAACCCAATTCTTGGGGCGAAAATTCAAGCTGTTTGTGAAGAGCTTGAGAGACAGGATTGGTTGGATTTGGTTCCGAAGCTATGGCCGAATTGTAAGTATGTTTACTCCATAATGACGGGGTCTATGCAGCCGTATTTGAAGAAATTGAGACGCTATGCGGGGAGGTTGCCGTTGGTGAGTGGGGATTATGGATCGACAGAGAGTTGGATTGGGGTTAATGTCGATCCTTGTCTGCCGCCTGAGAATGTTACATTTGCGGTCGTACCCACTTTCTCTTATTTTGAGTTCATCCCACTTTTCAGACAACATCAACAACATGATTGTGGCAGTTCCTCCGTTGCTGTCTTCGATGATTTCTTGGAAGGCCAACCCCTTCCCTTGTCTCAAGTCAAGATTGGACAGCAGTACGAACTTGTTCTCACCACTTTCACAGGTTAGTTCATTAATTATCAACACAAATCTAATCAAACGCCTTGCTTTTGCTCCTTCCTTATCATTATGTCATCCAGAGAAGATTAGTTTTTAAAACACGTTTGCTAGGGAGAGTTGCTTCGTTTGATCTCCCAATTCACTTCTCTTGGAGACCCACACATAGTTCAATGTCTAGTTTTGATACGAACTGTAATGACCCGACTAACTCTGACCGCTATGTGTAAGAAAAGTTGAGCAGTTACCATTAGTATCATGATTTGGTAATCATGAATCATTGATTTGATCAGTAATGATCAGTCCTATACGGGCAAGTTGAGAGAAATTTCCAACCTTCCTTATTTGGCTCCAAAATTTCCCAACTTTCCTTGTCAACATGATGCTTACATTGATTTCGCAAAGGTAAAATTTTGTACCAAGATTTGTTTTTTATTGAAATTTAGTGGGTGAATATCAATAAAGCCTAAAAAATGTGAAAACTTTTTTACTATAGTTTACCGTCACATCAAATATAGAGCATCTGATTGTAAATATGTAACCGCTCAAGCCCATTACTACTTGATATTATCCTCTTTAGACTTTCTCTCTCGAGCTTCCCCTAAAATGCATCTTTAGACTTTCACTTTCAAGCTTCCCCTCATTCTTTACTAAATTTTTCTGTCACATCAAATATAGAGCATATAGATCATATGATTTAATATATAATAGCTCGAGTATTATATTCTTTGTGTTTTTCTTTACGATCTTCTCTTCAAGGCTTTTAAGACATGTCTACTAGGAATAGGTTCTTACGTACTACTTATAAGGAGTGTTTCATTCTCATCTCTAAAAGAGGTGTTTAAACATAGTTCAGATGATTATGACATATATGTTTGATCTTCGACCCAAAGGGGTTAGTGGGTTTTGGTATTGTTTGCTCAACGGATGATTATGACATATATCTTCGATCGAAAGGGGTTAATGGGTTTTGGGTATTGGTTGTGATTGGCAGGACTATACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCAGGGTTTCACAACAAGACACCTAAACTCAACTTTATATGCAGAAGAAAGCTTGTATTAACCGTAAACATTGACAAGAACACCGAGAAGGATCTCCAATTGGTAGTGGAAAGAGGGTCCCAGCTGCTAGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACCAGCCATGCCGACTTAACCAACCAACCAGGCCACTATGTCATCTTCTGGGAAGTCAAAGGGCACGTCGAGGACGCAGTTCTTGGACACTGCTGCTCCGAGATGGACGCTGCTTTCGTGGACTACGGCTACGTGGTGTCCAGGCGGTCCAACACCATCGGACCCCTCGAGCTAAGGATCGTGGAAAGAGGCAGCTTCAACAAGATCATGGAACATTACATTGGTAACGGAGCTGCACTTAGCCAATTCAAGACACCCAGATGCACTACCAATCAGGTTCTTCTTACCATTCTCAATCTCTCCACTGTCAAAGCCTTTCATAGCACTGCTTATGGCTAAGTTTGGTTATTAGTATTGTTAGTATTTCCCAATTTTGAATCAGTTTCAGATCATTTCATTCTAACCAAATTAGAGTGTCACTAAACACTTACTCATCACCGCATGTAACGAGCCAAAACGAATAATATTTGTTAGTGGTGGGTTTCGGTTGTTACAATTAGTATCAGAGATAGACACCGAGCTGTCGTATCAGTTGGAGAAGAGAACAAATCATTCCTTATAGATGCTATTATAGTACCTCAAGAGTGAATAAAGTTTCGGTCGTCGTCGTCCTCGTCGTCTTGTTGTTTTTGCTTTTATCATCATAAAGCTGCCTCAACTCTTCTTCCACTGCTAGCTCTTAATGGGTTTGCTTTTGATTTCTACTAAAATCCTCCATTTGTGCATTATTTACTGCTACACCCCTCCCCTTTTCCGTCTCCTATGATATAATTATAAGGTTAAAAATGTAAATTTGTTAACTAATATTACTTTTTTT

mRNA sequence

CTGCTGCTGCTGCGGCTGCTGCGGCTGCGGGCGTGTTCAAGGCGGCGAGGTGCGCACCATGTTTGAAGAAGTAGAAGACATGGTTGATTGGTTCGAGCGAGTGTCGTCAGAAGCAGGCGTGGCTCAATTGCGCACACTCCGCGAGATTCTGCGCCAGAATTGTGGCGTGGAGTATCTAAACCAATGGCTTGGAGATGTCAATATCCATCAAATTCCTGATGACTTTGCATTGGAATCCCTCTTCACTTCCTTAATCCCCCTTTCTTCCCACGCTGATTTCGAGCCTTTTCTTCAACGAATCGCCGATGGTGACTCTTCCCCTTTGCTTACACAACAACCCATTCAAACTCTCTCTTTAAGTTCTGGCACCACCGAAGGCCGACAAAAGTACGTGCCCTTCACTCCACATAGTGCGCAGACTACCCTTCTCATCTTTCGTATAGCTGCTGCTTATAGATCCAGGTACCAATGCAATATGACATTAAAGAATGGCTTAATCACAACTAGGGTTTATCCCACAAGGGAAGGAGGCAGGATTTTGGAGTTCATTTACAGTAGTAAACAGACCAAAACCAAAGGAGGGATAACCACCGGCACAGCCACAACCCATTACTACGCCAGTGAGCAATTCAAAATCAAGCAGCTCAAAACCAAATCCTTCACTTGCAGCCCACAAGAAGTCATTTCCGGCGGTGATTACAAGCAATCCACTTACTGTCATCTTCTATTGGGGCTGTTCTTCTCCGACCAAGTCGAGTTTGTCACCTCAACTTTCGCTTACACCATAGTCCAAGCCTTCAACGAATTGGAAGATTGTTGGCCTCAAATCTGTCACGACATTACCCATGCCACACTCTGCTCTAGAATCACTCTTCCTGAAATCCGTAAGGCTGTTTTGAACATCATTAGTCCAAACCCAATTCTTGGGGCGAAAATTCAAGCTGTTTGTGAAGAGCTTGAGAGACAGGATTGGTTGGATTTGGTTCCGAAGCTATGGCCGAATTGTAAGTATGTTTACTCCATAATGACGGGGTCTATGCAGCCGTATTTGAAGAAATTGAGACGCTATGCGGGGAGGTTGCCGTTGGTGAGTGGGGATTATGGATCGACAGAGAGTTGGATTGGGGTTAATGTCGATCCTTGTCTGCCGCCTGAGAATGTTACATTTGCGGTCGTACCCACTTTCTCTTATTTTGAGTTCATCCCACTTTTCAGACAACATCAACAACATGATTGTGGCAGTTCCTCCGTTGCTGTCTTCGATGATTTCTTGGAAGGCCAACCCCTTCCCTTGTCTCAAGTCAAGATTGGACAGCAGTACGAACTTGTTCTCACCACTTTCACAGGACTATACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCAGGGTTTCACAACAAGACACCTAAACTCAACTTTATATGCAGAAGAAAGCTTGTATTAACCGTAAACATTGACAAGAACACCGAGAAGGATCTCCAATTGGTAGTGGAAAGAGGGTCCCAGCTGCTAGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACCAGCCATGCCGACTTAACCAACCAACCAGGCCACTATGTCATCTTCTGGGAAGTCAAAGGGCACGTCGAGGACGCAGTTCTTGGACACTGCTGCTCCGAGATGGACGCTGCTTTCGTGGACTACGGCTACGTGGTGTCCAGGCGGTCCAACACCATCGGACCCCTCGAGCTAAGGATCGTGGAAAGAGGCAGCTTCAACAAGATCATGGAACATTACATTGGTAACGGAGCTGCACTTAGCCAATTCAAGACACCCAGATGCACTACCAATCAGGTTCTTCTTACCATTCTCAATCTCTCCACTGTCAAAGCCTTTCATAGCACTGCTTATGGCTAAGTTTGGTTATTAGTATTGTTAGTATTTCCCAATTTTGAATCAGTTTCAGATCATTTCATTCTAACCAAATTAGAGTGTCACTAAACACTTACTCATCACCGCATGTAACGAGCCAAAACGAATAATATTTGTTAGTGGTGGGTTTCGGTTGTTACAATTAGTATCAGAGATAGACACCGAGCTGTCGTATCAGTTGGAGAAGAGAACAAATCATTCCTTATAGATGCTATTATAGTACCTCAAGAGTGAATAAAGTTTCGGTCGTCGTCGTCCTCGTCGTCTTGTTGTTTTTGCTTTTATCATCATAAAGCTGCCTCAACTCTTCTTCCACTGCTAGCTCTTAATGGGTTTGCTTTTGATTTCTACTAAAATCCTCCATTTGTGCATTATTTACTGCTACACCCCTCCCCTTTTCCGTCTCCTATGATATAATTATAAGGTTAAAAATGTAAATTTGTTAACTAATATTACTTTTTTT

Coding sequence (CDS)

ATGTTTGAAGAAGTAGAAGACATGGTTGATTGGTTCGAGCGAGTGTCGTCAGAAGCAGGCGTGGCTCAATTGCGCACACTCCGCGAGATTCTGCGCCAGAATTGTGGCGTGGAGTATCTAAACCAATGGCTTGGAGATGTCAATATCCATCAAATTCCTGATGACTTTGCATTGGAATCCCTCTTCACTTCCTTAATCCCCCTTTCTTCCCACGCTGATTTCGAGCCTTTTCTTCAACGAATCGCCGATGGTGACTCTTCCCCTTTGCTTACACAACAACCCATTCAAACTCTCTCTTTAAGTTCTGGCACCACCGAAGGCCGACAAAAGTACGTGCCCTTCACTCCACATAGTGCGCAGACTACCCTTCTCATCTTTCGTATAGCTGCTGCTTATAGATCCAGGTACCAATGCAATATGACATTAAAGAATGGCTTAATCACAACTAGGGTTTATCCCACAAGGGAAGGAGGCAGGATTTTGGAGTTCATTTACAGTAGTAAACAGACCAAAACCAAAGGAGGGATAACCACCGGCACAGCCACAACCCATTACTACGCCAGTGAGCAATTCAAAATCAAGCAGCTCAAAACCAAATCCTTCACTTGCAGCCCACAAGAAGTCATTTCCGGCGGTGATTACAAGCAATCCACTTACTGTCATCTTCTATTGGGGCTGTTCTTCTCCGACCAAGTCGAGTTTGTCACCTCAACTTTCGCTTACACCATAGTCCAAGCCTTCAACGAATTGGAAGATTGTTGGCCTCAAATCTGTCACGACATTACCCATGCCACACTCTGCTCTAGAATCACTCTTCCTGAAATCCGTAAGGCTGTTTTGAACATCATTAGTCCAAACCCAATTCTTGGGGCGAAAATTCAAGCTGTTTGTGAAGAGCTTGAGAGACAGGATTGGTTGGATTTGGTTCCGAAGCTATGGCCGAATTGTAAGTATGTTTACTCCATAATGACGGGGTCTATGCAGCCGTATTTGAAGAAATTGAGACGCTATGCGGGGAGGTTGCCGTTGGTGAGTGGGGATTATGGATCGACAGAGAGTTGGATTGGGGTTAATGTCGATCCTTGTCTGCCGCCTGAGAATGTTACATTTGCGGTCGTACCCACTTTCTCTTATTTTGAGTTCATCCCACTTTTCAGACAACATCAACAACATGATTGTGGCAGTTCCTCCGTTGCTGTCTTCGATGATTTCTTGGAAGGCCAACCCCTTCCCTTGTCTCAAGTCAAGATTGGACAGCAGTACGAACTTGTTCTCACCACTTTCACAGGACTATACAGATGCAGATTAGGAGACGTAGTAGAAGTAGCAGGGTTTCACAACAAGACACCTAAACTCAACTTTATATGCAGAAGAAAGCTTGTATTAACCGTAAACATTGACAAGAACACCGAGAAGGATCTCCAATTGGTAGTGGAAAGAGGGTCCCAGCTGCTAGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACCAGCCATGCCGACTTAACCAACCAACCAGGCCACTATGTCATCTTCTGGGAAGTCAAAGGGCACGTCGAGGACGCAGTTCTTGGACACTGCTGCTCCGAGATGGACGCTGCTTTCGTGGACTACGGCTACGTGGTGTCCAGGCGGTCCAACACCATCGGACCCCTCGAGCTAAGGATCGTGGAAAGAGGCAGCTTCAACAAGATCATGGAACATTACATTGGTAACGGAGCTGCACTTAGCCAATTCAAGACACCCAGATGCACTACCAATCAGGTTCTTCTTACCATTCTCAATCTCTCCACTGTCAAAGCCTTTCATAGCACTGCTTATGGCTAA

Protein sequence

MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAYG
BLAST of Carg03169 vs. NCBI nr
Match: XP_023512179.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1200.7 bits (3105), Expect = 0.0e+00
Identity = 589/608 (96.88%), Postives = 591/608 (97.20%), Query Frame = 0

Query: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180
           TTLLIFRIAAAYRS               RVYPTREGGRILEFIYSSKQTKTKGGITTGT
Sbjct: 121 TTLLIFRIAAAYRS---------------RVYPTREGGRILEFIYSSKQTKTKGGITTGT 180

Query: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240
           ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA
Sbjct: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240

Query: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300
           YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEEL
Sbjct: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEEL 300

Query: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360
           ERQDWLDLVPKLWPNCKYVYSIMTGS+QPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD
Sbjct: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360

Query: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ 420
           PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVA FDDFLEGQPLPLSQVKIGQQ
Sbjct: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQ 420

Query: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480
           YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER
Sbjct: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480

Query: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540
           GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY
Sbjct: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540

Query: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 600
           VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK
Sbjct: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 593

Query: 601 AFHSTAYG 609
           AFHSTAYG
Sbjct: 601 AFHSTAYG 593

BLAST of Carg03169 vs. NCBI nr
Match: XP_022943441.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata])

HSP 1 Score: 1199.9 bits (3103), Expect = 0.0e+00
Identity = 589/608 (96.88%), Postives = 591/608 (97.20%), Query Frame = 0

Query: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFE+VEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPI+TLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180
           TTLLIFRIAAAYRS               RVYPTREGGRILEFIYSSKQTKTKGGITTGT
Sbjct: 121 TTLLIFRIAAAYRS---------------RVYPTREGGRILEFIYSSKQTKTKGGITTGT 180

Query: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240
           ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA
Sbjct: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240

Query: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300
           YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKI AVCEEL
Sbjct: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEEL 300

Query: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360
           ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD
Sbjct: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360

Query: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ 420
           PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVA FDDFLEGQPLPLSQVKIGQQ
Sbjct: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQ 420

Query: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480
           YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER
Sbjct: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480

Query: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540
           GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY
Sbjct: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540

Query: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 600
           VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK
Sbjct: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 593

Query: 601 AFHSTAYG 609
           AFHSTAYG
Sbjct: 601 AFHSTAYG 593

BLAST of Carg03169 vs. NCBI nr
Match: XP_022986272.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima])

HSP 1 Score: 1189.5 bits (3076), Expect = 0.0e+00
Identity = 584/608 (96.05%), Postives = 588/608 (96.71%), Query Frame = 0

Query: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           MFEEVEDMVDWFERVSSEAGVAQ RTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES
Sbjct: 1   MFEEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
           LFTSL+PLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ
Sbjct: 61  LFTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120

Query: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180
           TTLLIFRIAAAYRS               RVYPTREGGRILEFIYSSKQTKTKGGITTGT
Sbjct: 121 TTLLIFRIAAAYRS---------------RVYPTREGGRILEFIYSSKQTKTKGGITTGT 180

Query: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240
           ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA
Sbjct: 181 ATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFA 240

Query: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300
           YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL
Sbjct: 241 YTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEEL 300

Query: 301 ERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVD 360
           ERQDWLDLVPKLW NCKYVYSIMTGSMQPYLKKLRRYAG LPLVSGDYGSTESWIG+NVD
Sbjct: 301 ERQDWLDLVPKLWSNCKYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVD 360

Query: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQ 420
           PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVA FDDFLEGQPLPLSQVKIGQQ
Sbjct: 361 PCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQ 420

Query: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480
           YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER
Sbjct: 421 YELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVER 480

Query: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGY 540
           GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKG+V+DAVLG CCSEMDAAFVDYGY
Sbjct: 481 GSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGY 540

Query: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 600
           VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK
Sbjct: 541 VVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVK 593

Query: 601 AFHSTAYG 609
           AFHSTAYG
Sbjct: 601 AFHSTAYG 593

BLAST of Carg03169 vs. NCBI nr
Match: XP_008448228.1 (PREDICTED: jasmonic acid-amido synthetase JAR1 isoform X1 [Cucumis melo])

HSP 1 Score: 1008.1 bits (2605), Expect = 1.3e-290
Identity = 493/605 (81.49%), Postives = 540/605 (89.26%), Query Frame = 0

Query: 3   EEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 62
           EE ED++DWFE+VSSEA VAQ RTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L+S+F
Sbjct: 8   EEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLQSIF 67

Query: 63  TSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTT 122
           TSLIPLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTPHSA TT
Sbjct: 68  TSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAHTT 127

Query: 123 LLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTAT 182
           LLIFR+AAAYRS               RVYPTREGG+ILEFIYSSKQTKTKGGITTGTAT
Sbjct: 128 LLIFRLAAAYRS---------------RVYPTREGGKILEFIYSSKQTKTKGGITTGTAT 187

Query: 183 THYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYT 242
           THYYASE+FKIKQLKTKSFTCSPQEVISG DYKQSTYCHLLLGL +S++VEFVTSTFAYT
Sbjct: 188 THYYASEEFKIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYT 247

Query: 243 IVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELER 302
           IVQAFN+LE+ W +I HDI+HATL SRI +PEIRKAVL ++SP P LG KI+ VCEEL++
Sbjct: 248 IVQAFNQLEESWEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKK 307

Query: 303 QDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPC 362
           + WL L+PKLWPNCKYVYSIMTGSMQPYLKKLR Y G LPLVSGDYGSTESWIGVNVDP 
Sbjct: 308 EGWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPY 367

Query: 363 LPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYE 422
           LPPENVTFAV+PTFSYFEFIPLF+QH      SS+     DFLEGQP+ LS+VKIGQQYE
Sbjct: 368 LPPENVTFAVIPTFSYFEFIPLFKQHA---TTSSTDTAIHDFLEGQPIALSEVKIGQQYE 427

Query: 423 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGS 482
           LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGS
Sbjct: 428 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGS 487

Query: 483 QLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVV 542
           QL++  SGAELVDFTS+A+L+NQPGHYVIFWEVKG V D VLG CCSEMDAAFVDYGYVV
Sbjct: 488 QLMSQLSGAELVDFTSYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVV 547

Query: 543 SRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAF 602
           SR++N+IGPLELRIVERGSFNKI+E+YIGNGAALSQFKTPRCTTN  LL ILNLST+++F
Sbjct: 548 SRKANSIGPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSF 594

Query: 603 HSTAY 608
            STAY
Sbjct: 608 FSTAY 594

BLAST of Carg03169 vs. NCBI nr
Match: XP_004140048.1 (PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.5 [Cucumis sativus] >KGN46697.1 hypothetical protein Csa_6G124120 [Cucumis sativus])

HSP 1 Score: 994.6 bits (2570), Expect = 1.5e-286
Identity = 486/605 (80.33%), Postives = 535/605 (88.43%), Query Frame = 0

Query: 3   EEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 62
           E  ED++ WFE+VSSEA +AQ RTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L S+F
Sbjct: 2   EGEEDIIGWFEQVSSEAALAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLHSIF 61

Query: 63  TSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTT 122
           TS IPLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTPHSAQTT
Sbjct: 62  TSSIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAQTT 121

Query: 123 LLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTAT 182
           LLIFR+AAAYRS               RVYP R+GG+ILEFIYSSKQTKTKGGITTGTAT
Sbjct: 122 LLIFRLAAAYRS---------------RVYPIRDGGKILEFIYSSKQTKTKGGITTGTAT 181

Query: 183 THYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYT 242
           THYYASE+FKIKQLKTKSFTCSPQEVI+G DYKQSTYCHLLLGL +S++VEFVTSTFAYT
Sbjct: 182 THYYASEEFKIKQLKTKSFTCSPQEVITGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYT 241

Query: 243 IVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELER 302
           IVQAFN+LE+ W ++ HD++HATL SRI +PEIRKAVL ++SP P LG KI+ VCEEL +
Sbjct: 242 IVQAFNQLEESWEEMIHDLSHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELMK 301

Query: 303 QDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPC 362
           + WL L+PKLWPNCKYVYSIMTGSMQPYLKKLR Y G LPLVSGDYGSTESWIGVNVDP 
Sbjct: 302 EGWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPY 361

Query: 363 LPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYE 422
           LPPENVTFAV+PTFSYFEFIPLF+QH      SS+     DFLEGQP+ LS+VKIGQQYE
Sbjct: 362 LPPENVTFAVIPTFSYFEFIPLFKQHA---TTSSADTAIHDFLEGQPIALSEVKIGQQYE 421

Query: 423 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGS 482
           LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGS
Sbjct: 422 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGS 481

Query: 483 QLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVV 542
           QL++  SGAELVDFTS+A+L+NQPGHYVIFWE+KG V D VLG CCSEMDAAFVDYGYVV
Sbjct: 482 QLMSQLSGAELVDFTSYAELSNQPGHYVIFWELKGDVNDKVLGMCCSEMDAAFVDYGYVV 541

Query: 543 SRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAF 602
           SR++N+IGPLELRIVERGSFNKI+EHYIGNGAALSQFKTPRCTTN  LL ILNLST+K+F
Sbjct: 542 SRKANSIGPLELRIVERGSFNKILEHYIGNGAALSQFKTPRCTTNHFLLNILNLSTLKSF 588

Query: 603 HSTAY 608
            STAY
Sbjct: 602 FSTAY 588

BLAST of Carg03169 vs. TAIR10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 811.2 bits (2094), Expect = 4.3e-235
Identity = 396/603 (65.67%), Postives = 471/603 (78.11%), Query Frame = 0

Query: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65
           +D++ WFE VS  A   Q  TLR IL  N GVEYL +WLG V++ ++ DD+ LE+LFTSL
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM-DDYTLETLFTSL 68

Query: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125
           +P+ SHAD +P++QRIADG++SPLLTQ+PI  LSLSSGTTEGRQKYVPFT HSAQTTL I
Sbjct: 69  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 128

Query: 126 FRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHY 185
           FR++AAYRSR+               YP REGGRILEFIY+ K+ KT GG+T GTATTHY
Sbjct: 129 FRLSAAYRSRF---------------YPIREGGRILEFIYAGKEFKTLGGLTVGTATTHY 188

Query: 186 YASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQ 245
           YASE+FK KQ  TKSFTCSPQEVISGGD+ Q TYCHLLLGL +S QVEFV S F+YTIVQ
Sbjct: 189 YASEEFKTKQETTKSFTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQ 248

Query: 246 AFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQ-D 305
           AF+  E+ W +IC DI    L SRITLP++RKAVL +I PNP L + I+ +C ELE    
Sbjct: 249 AFSFFEEIWREICADIKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLG 308

Query: 306 WLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLP 365
           W  L+ KLWPN K++ SIMTGSM PYL KLR YAG LPLVS DYGSTESWIGVNVDP LP
Sbjct: 309 WFGLISKLWPNAKFISSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLP 368

Query: 366 PENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYELV 425
           PE+V+FAV+PTFSYFEFIPL+R+  Q D     + +  DF+E +P+PLSQVK+GQ+YELV
Sbjct: 369 PEDVSFAVIPTFSYFEFIPLYRRQNQSD-----ICIDGDFVEDKPVPLSQVKLGQEYELV 428

Query: 426 LTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQL 485
           LTTFTGLYR RLGDVVEV  FH  TPKL+FI RRKL+LT+NIDKNTEKDLQ VV++ SQL
Sbjct: 429 LTTFTGLYRYRLGDVVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQL 488

Query: 486 LADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSR 545
           L+  + AE+VDFTSHAD+  +PGHYVI+WE++G  +D  L  CC EMD AFVDYGYVVSR
Sbjct: 489 LSRSTRAEVVDFTSHADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSR 548

Query: 546 RSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHS 605
           R N+IGPLELR+VERG+F K+ E  +G    L+QFKTPRCTTN V+L ILN ST+K F S
Sbjct: 549 RMNSIGPLELRVVERGTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRS 590

Query: 606 TAY 608
           +AY
Sbjct: 609 SAY 590

BLAST of Carg03169 vs. TAIR10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein)

HSP 1 Score: 538.1 bits (1385), Expect = 7.0e-153
Identity = 276/614 (44.95%), Postives = 387/614 (63.03%), Query Frame = 0

Query: 1   MFEEVED-----MVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDD 60
           M E+VE      ++D F+ ++  A   Q +TL+EIL +N    YL       N     + 
Sbjct: 12  MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNA 71

Query: 61  FALESLFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFT 120
              E  F S++PL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT
Sbjct: 72  TDPEEAFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFT 131

Query: 121 PHSAQTTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGG 180
               + TL +FR A A+R+               R +P  + G+ L+FI+SSKQ  + GG
Sbjct: 132 DELMENTLQLFRTAFAFRN---------------RDFPIDDNGKALQFIFSSKQYISTGG 191

Query: 181 ITTGTATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFV 240
           +  GTATT+ Y +  FK       S +CSP EVI   D  Q+ YCHLL G+ F DQV++V
Sbjct: 192 VPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYV 251

Query: 241 TSTFAYTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQA 300
            + FA+ +V AF   E  W +I  DI    L +RIT+P +R A+  +++PNP L   I+ 
Sbjct: 252 FAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRT 311

Query: 301 VCEELERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWI 360
            C  L   +W  L+P L+PN KYVY IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI
Sbjct: 312 KCMSL--SNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWI 371

Query: 361 GVNVDPCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQV 420
             NV P L PE  TFAV+P   YFEF+P+       + G           E +P+ L+QV
Sbjct: 372 AANVTPRLSPEEATFAVIPNLGYFEFLPV------SETGEG---------EEKPVGLTQV 431

Query: 421 KIGQQYELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQ 480
           KIG++YE+V+T + GLYR RLGDVV+V GF+N TP+L FICRR L+L++NIDKNTE+DLQ
Sbjct: 432 KIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQ 491

Query: 481 LVVERGSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAF 540
           L VE  ++ L+++   E++DF+S+ D++  PGHY IFWE+ G   + VL  CC+ +D AF
Sbjct: 492 LSVESAAKRLSEEK-IEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAF 551

Query: 541 VDYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTI 600
           +D GYV SR+  TIG LELR+V +G+F KI EH++G G++  QFK PRC   +N  +L I
Sbjct: 552 IDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQI 586

Query: 601 LNLSTVKAFHSTAY 608
           L  + V ++ STA+
Sbjct: 612 LCENVVSSYFSTAF 586

BLAST of Carg03169 vs. TAIR10
Match: AT1G28130.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 372.5 bits (955), Expect = 5.1e-103
Identity = 215/606 (35.48%), Postives = 342/606 (56.44%), Query Frame = 0

Query: 13  ERVSSEAGVAQLRTLREILRQNCGVEYLNQWL-GDVNIHQIPDDFALESLFTSLIPLSSH 72
           E +++ A   Q + L +IL QN G +YL  +L G+ + +Q          F + +P+ ++
Sbjct: 18  EDLTTNAEAIQQQVLHQILSQNSGTQYLRAFLDGEADKNQ--------QSFKNKVPVVNY 77

Query: 73  ADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRIAAA 132
            D +PF+QRIADG+SS +++ QPI  L  SSGT+ G+ K +P T    +     + +   
Sbjct: 78  DDVKPFIQRIADGESSDIVSAQPITELLTSSGTSAGKPKLMPSTAEELERKTFFYSMLVP 137

Query: 133 YRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQF 192
             ++Y       +GL         + G+ +  ++   + KT  G+      T YY S+ F
Sbjct: 138 IMNKY------VDGL---------DEGKGMYLLFIKPEIKTPSGLMARPVLTSYYKSQHF 197

Query: 193 KIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELE 252
           + +     +   SP + I   D KQS YC LL GL     V  V + FA   ++A   LE
Sbjct: 198 RNRPFNKYNVYTSPDQTILCQDSKQSMYCQLLCGLVQRSHVLRVGAVFASAFLRAVKFLE 257

Query: 253 DCWPQICHDITHATLCSRITLPEIRKAVLNIIS-PNPILGAKIQAVCEELERQDWLDLVP 312
           D + ++C DI   T+ S IT    R +VL+I++ PN  L  +I++ C E   + W  ++ 
Sbjct: 258 DHYKELCADIRTGTVTSWITDSSCRDSVLSILNGPNQELADEIESECAE---KSWEGILR 317

Query: 313 KLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTF 372
           ++WP  KYV  I+TGSM  Y+  L  Y+G LPLVS  Y S+E + G+N++P   P +V++
Sbjct: 318 RIWPKAKYVEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINLNPLCDPADVSY 377

Query: 373 AVVPTFSYFEFIPL-FRQHQQ-HDCGSSSVAVFDDFL-EGQPLPLSQVKIGQQYELVLTT 432
            ++P  +YFEF+P+  + H++ H    S+    DD L E   + L  V++GQ YE+V+TT
Sbjct: 378 TLLPNMAYFEFLPVDDKSHEEIHFATHSNTDDDDDALKEDLIVNLVNVEVGQYYEIVITT 437

Query: 433 FTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD-LQLVVERGSQLLA 492
           FTGLYR R+GD+++V GFHNK P+  F+ RR +VL+++ DK +E+D L  V +     L 
Sbjct: 438 FTGLYRYRVGDILKVTGFHNKAPQFRFVQRRNVVLSIDTDKTSEEDLLNAVTQAKLNHLQ 497

Query: 493 DQSGAELVDFTSHADLTNQPGHYVIFWEVKGH-------VEDAVLGHCCSEMDAAFVDYG 552
             S   L ++TS+AD ++ PGHYV+FWE+K         ++D  +  CCSE++   +DY 
Sbjct: 498 HPSSLLLTEYTSYADTSSIPGHYVLFWELKPRHSNDPPKLDDKTMEDCCSEVEDC-LDYV 557

Query: 553 YVVSR-RSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLST 605
           Y   R R  +IGPLE+R+V  G+F+ +M+  +  G++L+Q+KTPRC  +   L IL+   
Sbjct: 558 YRRCRNRDKSIGPLEIRVVSLGTFDSLMDFCVSQGSSLNQYKTPRCVKSGGALEILDSRV 596

BLAST of Carg03169 vs. TAIR10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein)

HSP 1 Score: 360.5 bits (924), Expect = 2.0e-99
Identity = 199/602 (33.06%), Postives = 328/602 (54.49%), Query Frame = 0

Query: 11  WFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSLIPLSS 70
           + E ++      Q + L EIL +N   EYL ++  D+      D       F S +P+ +
Sbjct: 26  FIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRF--DL------DGVVDRKTFKSKVPVVT 85

Query: 71  HADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRIAA 130
           + D +P +QRI++GD SP+L+  PI     SSGT+ G +K +P          L++ +  
Sbjct: 86  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 145

Query: 131 AYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQ 190
              + Y                P  + G+ L F++   ++KT GG+      T YY S+ 
Sbjct: 146 PVMNLY---------------VPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDH 205

Query: 191 FKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNEL 250
           FK +     +   SP E I   D  QS Y  +L GL    +V  + + FA  +++A + L
Sbjct: 206 FKRRPYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFL 265

Query: 251 EDCWPQICHDITHATLCSRITLPEIRKAVLNIIS-PNPILGAKIQAVCEELERQDWLDLV 310
           ++ W ++  DI+  TL SRI  P I+  +  I++ P+  L   +  VC +   ++W  ++
Sbjct: 266 QNNWKELARDISTGTLSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQ---ENWEGII 325

Query: 311 PKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVT 370
            K+WPN KY+  I+TG+M  Y+  L  Y+G LP+    Y S+ES+ G+N+ P   P  V+
Sbjct: 326 TKIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVS 385

Query: 371 FAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYELVLTTFT 430
           + ++P  +YFEF+P       HD   ++ A  D   E   + L+ V++G++YELV+TT+ 
Sbjct: 386 YTIMPNMAYFEFLP-----HNHDGDGAAEASLD---ETSLVELANVEVGKEYELVITTYA 445

Query: 431 GLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQS 490
           GLYR R+GD++ V GFHN  P+  FI R+ ++L+V  DK  E +LQ  VE  S+L A+Q 
Sbjct: 446 GLYRYRVGDILRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQ- 505

Query: 491 GAELVDFTSHADLTNQPGHYVIFWEVKGH------VEDAVLGHCCSEMDAAFVDYGYVVS 550
           G  ++++TS+A+    PGHYVI+WE+ G       + + V+  CC EM+ + ++  Y  S
Sbjct: 506 GTRVIEYTSYAETKTIPGHYVIYWELLGRDQSNALMSEEVMAKCCLEMEES-LNSVYRQS 565

Query: 551 R-RSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAF 605
           R    +IGPLE+R+V  G+F ++M++ I  GA+++Q+K PRC +   ++ +L+   V A 
Sbjct: 566 RVADKSIGPLEIRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAH 591

BLAST of Carg03169 vs. TAIR10
Match: AT2G47750.1 (putative indole-3-acetic acid-amido synthetase GH3.9)

HSP 1 Score: 358.6 bits (919), Expect = 7.6e-99
Identity = 206/601 (34.28%), Postives = 330/601 (54.91%), Query Frame = 0

Query: 13  ERVSSEAGVAQLRTLREILRQNCGVEYLNQWL-GDVNIHQIPDDFALESLFTSLIPLSSH 72
           ER++S+A   Q   LR IL +N   EYL++++ G  ++ +          F   +P+  +
Sbjct: 16  ERITSKAAEVQDNILRGILERNKDTEYLSKYMNGSKDVLE----------FKRAVPIIIY 75

Query: 73  ADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRIAAA 132
            D  P++QRIA+G+ S L+T   I  +  SSGT+ G  K +P  P        ++ +   
Sbjct: 76  KDIYPYIQRIANGEDSSLITGHSITEILCSSGTSAGEPKLMPTIPEDLDRRTFLYNLIIP 135

Query: 133 YRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQF 192
             ++Y   +               + G+ +   +   +T T  G+      T YY S+ F
Sbjct: 136 IVNKYITGL---------------DKGKAMYLNFVKAETSTPCGLPIRAVLTSYYKSKHF 195

Query: 193 KIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELE 252
           + +     +   SP + I   D  QS YC LL GL    +V  + + FA   ++A + LE
Sbjct: 196 QCRPYDPFNDLTSPIQTILCEDSNQSMYCQLLAGLIHRHKVMRLGAVFASAFLRAISYLE 255

Query: 253 DCWPQICHDITHATLCSRITLPEIRKAV-LNIISPNPILGAKIQAVCEELERQDWLDLVP 312
             W Q+C DI   +L   IT P  + A+   ++SPNP L ++I+ +C    R  W  ++ 
Sbjct: 256 KKWSQLCEDIRTGSLNPMITDPGCQMAMSCLLMSPNPELASEIEEIC---GRSSWKGILC 315

Query: 313 KLWPNCKYVYSIMTGSMQPYLKKLRRYA-GRLPLVSGDYGSTESWIGVNVDPCLPPENVT 372
           +LWP  K++ +++TGSM  Y+  L  ++ G++PLV   Y S+E++ GVNV+P   P +V 
Sbjct: 316 QLWPKAKFIEAVVTGSMAQYIPALEFFSQGKIPLVCPMYASSETYFGVNVEPLSKPSDVV 375

Query: 373 FAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQP----LPLSQVKIGQQYELVL 432
           F ++P   YFEFIPL +       G+ S  + DD  E  P    + L  VK+G+ YELV+
Sbjct: 376 FTLLPNMCYFEFIPLGKN------GTLSFDLDDD--EQVPCDKVVDLVNVKLGRYYELVV 435

Query: 433 TTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLL 492
           TTF GLYR R+GDV++VAGF+N  P+  FICRR +VL++++DK  E+DL   +    + L
Sbjct: 436 TTFAGLYRYRIGDVLQVAGFYNGAPQFRFICRRNVVLSIDLDKTNEEDLHRSITLAKKKL 495

Query: 493 ADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSR- 552
              S A L ++TS+AD ++ PGHYV+FWE++GH+E  ++  CC  ++   +DY Y   R 
Sbjct: 496 G--SNAFLAEYTSYADTSSVPGHYVLFWEIQGHLEPKLMEECCVAVEEE-LDYIYRQCRT 555

Query: 553 RSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT-TNQVLLTILNLSTVKAFH 605
           +  +IG LE+R+V+ G+F K+M+  I  G + +Q+KTPRC  +N     +LN   + +F 
Sbjct: 556 KERSIGALEIRVVKPGTFEKLMDLIISQGGSFNQYKTPRCVKSNSATFKLLNGHVMASFF 577

BLAST of Carg03169 vs. Swiss-Prot
Match: sp|Q9ZNS2|GH310_ARATH (Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=GH3.10 PE=2 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 7.7e-234
Identity = 396/603 (65.67%), Postives = 471/603 (78.11%), Query Frame = 0

Query: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65
           +D++ WFE VS  A   Q  TLR IL  N GVEYL +WLG V++ ++ DD+ LE+LFTSL
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM-DDYTLETLFTSL 68

Query: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125
           +P+ SHAD +P++QRIADG++SPLLTQ+PI  LSLSSGTTEGRQKYVPFT HSAQTTL I
Sbjct: 69  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 128

Query: 126 FRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHY 185
           FR++AAYRSR+               YP REGGRILEFIY+ K+ KT GG+T GTATTHY
Sbjct: 129 FRLSAAYRSRF---------------YPIREGGRILEFIYAGKEFKTLGGLTVGTATTHY 188

Query: 186 YASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQ 245
           YASE+FK KQ  TKSFTCSPQEVISGGD+ Q TYCHLLLGL +S QVEFV S F+YTIVQ
Sbjct: 189 YASEEFKTKQETTKSFTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQ 248

Query: 246 AFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQ-D 305
           AF+  E+ W +IC DI    L SRITLP++RKAVL +I PNP L + I+ +C ELE    
Sbjct: 249 AFSFFEEIWREICADIKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLG 308

Query: 306 WLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLP 365
           W  L+ KLWPN K++ SIMTGSM PYL KLR YAG LPLVS DYGSTESWIGVNVDP LP
Sbjct: 309 WFGLISKLWPNAKFISSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLP 368

Query: 366 PENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYELV 425
           PE+V+FAV+PTFSYFEFIPL+R+  Q D     + +  DF+E +P+PLSQVK+GQ+YELV
Sbjct: 369 PEDVSFAVIPTFSYFEFIPLYRRQNQSD-----ICIDGDFVEDKPVPLSQVKLGQEYELV 428

Query: 426 LTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQL 485
           LTTFTGLYR RLGDVVEV  FH  TPKL+FI RRKL+LT+NIDKNTEKDLQ VV++ SQL
Sbjct: 429 LTTFTGLYRYRLGDVVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQL 488

Query: 486 LADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSR 545
           L+  + AE+VDFTSHAD+  +PGHYVI+WE++G  +D  L  CC EMD AFVDYGYVVSR
Sbjct: 489 LSRSTRAEVVDFTSHADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSR 548

Query: 546 RSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHS 605
           R N+IGPLELR+VERG+F K+ E  +G    L+QFKTPRCTTN V+L ILN ST+K F S
Sbjct: 549 RMNSIGPLELRVVERGTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRS 590

Query: 606 TAY 608
           +AY
Sbjct: 609 SAY 590

BLAST of Carg03169 vs. Swiss-Prot
Match: sp|Q6I581|GH35_ORYSJ (Jasmonic acid-amido synthetase JAR1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=2 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 1.3e-159
Identity = 287/606 (47.36%), Postives = 392/606 (64.69%), Query Frame = 0

Query: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYL-NQWLGDVNIHQIPDDFALESLFTS 65
           E+ ++ FE ++ +A   Q  TL++IL  N   EYL N  LG     +          + S
Sbjct: 7   EETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLGGRTDAE---------SYKS 66

Query: 66  LIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLL 125
            IPL  H D EP++QRI DGD+SP++T +PI  LSLSSGTT G+ K++PF     +TTL 
Sbjct: 67  CIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLETTLQ 126

Query: 126 IFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTH 185
           I+R + A+R+               R YP  + G+ L+F+Y SKQ  TKGGI   TATT+
Sbjct: 127 IYRTSYAFRN---------------REYPIGQ-GKALQFVYGSKQVITKGGILATTATTN 186

Query: 186 YYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIV 245
            Y  +++K      +S  CSP EVI G D+ QS YCHLL GL +S++V  V STFA+++V
Sbjct: 187 LYRRQRYKEGMKDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLV 246

Query: 246 QAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQD 305
            AF   E+ W  +C DI    L  ++T P IR+AV  I+ PNP L   I   C  L   +
Sbjct: 247 HAFQTFEEVWEDLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIGL--SN 306

Query: 306 WLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLP 365
           W  ++P LWPN KYVY IMTGSM+PYLKKLR YAG LPL+S DYG++E W+G N+DP +P
Sbjct: 307 WYGVIPALWPNAKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNIDPTVP 366

Query: 366 PENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYELV 425
           PE VT+AV+P   YFEFIPL +   +    S+S+     ++E  P+ L++V++G+ YE+V
Sbjct: 367 PEQVTYAVLPQVGYFEFIPLEKPIGEETENSASI----HYIESDPVGLTEVEVGKIYEVV 426

Query: 426 LTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQL 485
           +T F GLYR RLGDVV++A FHN TP+L FICRR LVL++NIDKNTEKDLQL VE  S+ 
Sbjct: 427 ITNFAGLYRYRLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVEEASKF 486

Query: 486 LADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSR 545
           L  +   E++DFTS  + ++ PG YVIFWE+ G   D VL  C + +D AF+D GY  SR
Sbjct: 487 LEGEK-LEVMDFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDAGYTGSR 546

Query: 546 RSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTILNLSTVKAF 605
           +  TIGPLELRI+ +G+F +I++H++  G A+SQFKTPR    +N  +L IL+ +  +++
Sbjct: 547 KIKTIGPLELRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQILSRNVTQSY 580

Query: 606 HSTAYG 609
            STAYG
Sbjct: 607 FSTAYG 580

BLAST of Carg03169 vs. Swiss-Prot
Match: sp|Q9SKE2|JAR1_ARATH (Jasmonic acid-amido synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 538.1 bits (1385), Expect = 1.3e-151
Identity = 276/614 (44.95%), Postives = 387/614 (63.03%), Query Frame = 0

Query: 1   MFEEVED-----MVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDD 60
           M E+VE      ++D F+ ++  A   Q +TL+EIL +N    YL       N     + 
Sbjct: 1   MLEKVETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQ------NCGLNGNA 60

Query: 61  FALESLFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFT 120
              E  F S++PL +  + EP+++R+ DGD+SP+LT  P+  +SLSSGT++GR K++PFT
Sbjct: 61  TDPEEAFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFT 120

Query: 121 PHSAQTTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGG 180
               + TL +FR A A+R+               R +P  + G+ L+FI+SSKQ  + GG
Sbjct: 121 DELMENTLQLFRTAFAFRN---------------RDFPIDDNGKALQFIFSSKQYISTGG 180

Query: 181 ITTGTATTHYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFV 240
           +  GTATT+ Y +  FK       S +CSP EVI   D  Q+ YCHLL G+ F DQV++V
Sbjct: 181 VPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYV 240

Query: 241 TSTFAYTIVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQA 300
            + FA+ +V AF   E  W +I  DI    L +RIT+P +R A+  +++PNP L   I+ 
Sbjct: 241 FAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRT 300

Query: 301 VCEELERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWI 360
            C  L   +W  L+P L+PN KYVY IMTGSM+PY+ KLR YAG LPLVS DYGS+E WI
Sbjct: 301 KCMSL--SNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWI 360

Query: 361 GVNVDPCLPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQV 420
             NV P L PE  TFAV+P   YFEF+P+       + G           E +P+ L+QV
Sbjct: 361 AANVTPRLSPEEATFAVIPNLGYFEFLPV------SETGEG---------EEKPVGLTQV 420

Query: 421 KIGQQYELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQ 480
           KIG++YE+V+T + GLYR RLGDVV+V GF+N TP+L FICRR L+L++NIDKNTE+DLQ
Sbjct: 421 KIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQ 480

Query: 481 LVVERGSQLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAF 540
           L VE  ++ L+++   E++DF+S+ D++  PGHY IFWE+ G   + VL  CC+ +D AF
Sbjct: 481 LSVESAAKRLSEEK-IEVIDFSSYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAF 540

Query: 541 VDYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCT--TNQVLLTI 600
           +D GYV SR+  TIG LELR+V +G+F KI EH++G G++  QFK PRC   +N  +L I
Sbjct: 541 IDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQI 575

Query: 601 LNLSTVKAFHSTAY 608
           L  + V ++ STA+
Sbjct: 601 LCENVVSSYFSTAF 575

BLAST of Carg03169 vs. Swiss-Prot
Match: sp|Q53P49|GH312_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.12 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.12 PE=2 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 1.4e-142
Identity = 264/627 (42.11%), Postives = 390/627 (62.20%), Query Frame = 0

Query: 1   MFEEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALES 60
           M  E E+++ WFER + +A   Q  TLR IL +N GVEYL     ++ +  + D      
Sbjct: 10  MSREDEEIMAWFERTTRDAADVQRETLRRILAENAGVEYLR----ELGLAGLTD----AG 69

Query: 61  LFTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQ 120
            F + +P+ +HAD +P++QR+ADGD+SP+LT +P+  +SLSSGTT+G++K + F     +
Sbjct: 70  SFRARVPVVTHADLDPYIQRVADGDASPVLTAKPVTAISLSSGTTQGKRKRLLFNDDLLR 129

Query: 121 TTLLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGT 180
           +++  F  + A+               T R +P  E GR+L+F+Y S+   TKGG+T  T
Sbjct: 130 SSIRFFHASYAF---------------TNRAFPV-EDGRVLQFMYGSRHETTKGGLTATT 189

Query: 181 ATTHYYASEQFKIK---QLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTS 240
             T+   SE+F      + + +  +CSP EV+   D+ +S YCHLL GL  + +V  V++
Sbjct: 190 VMTNLLRSEEFTASMAARSRPRLPSCSPSEVVFSPDFDESLYCHLLCGLLLAGEVRAVSA 249

Query: 241 TFAYTIVQAFNELEDCWPQICHDITH-ATLCSRITLPEIRKAVLNII-SPNPILGAKIQA 300
           +FA++IV A   LE  W ++C DI   A   +R+T P +R+AV  I+ +PNP L   ++ 
Sbjct: 250 SFAHSIVVALQALERVWRELCADIRRGAASPARVTTPAVRRAVAPILAAPNPALADALER 309

Query: 301 VCEELERQDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWI 360
            C  L   DW  ++P LWPN +YV + MTGSM+ Y+KKLR YAG +PLVSG+Y S+E  I
Sbjct: 310 RCAAL--GDWSGVIPALWPNARYVQATMTGSMEHYVKKLRHYAGGVPLVSGNYASSEGVI 369

Query: 361 GVNVDPCLPPENVTFAVVPTFSYFEFIPL-----FRQHQQHDCGSSSVAVFDDFLEGQPL 420
           G+N +   PPE+V F V+P  +YFEFIPL            +  ++  + + D  +  P+
Sbjct: 370 GINAEQHAPPESVVFTVLPDAAYFEFIPLKPPCTDAAADDDNPAAAGSSCYVDADDANPV 429

Query: 421 PLSQVKIGQQYELVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNT 480
            L+ V +G+ YE+V+TTFTGLYR RLGDVV+VAGFH+ TPKL F+CRR L+L++N+DKN+
Sbjct: 430 GLTDVVVGEHYEVVMTTFTGLYRYRLGDVVKVAGFHHATPKLRFVCRRSLILSINVDKNS 489

Query: 481 EKDLQLVVERGSQLLA----DQSGAELVDFTSHADLTNQPGHYVIFWEVKG---HVEDAV 540
           E DLQL V+  +++LA    +    E+ D+TSHAD ++ PGHYV+FWE+ G        V
Sbjct: 490 EHDLQLAVDSAAKILAGDGENHKQLEIADYTSHADTSSDPGHYVVFWELNGGXXXXXXXV 549

Query: 541 LGHCCSEMDAAF-VDYGYVVSRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTP 600
           L  CC EMD AF  D GY  SR++  IG LELR++ RG+F +++ HY+  G++  QFK P
Sbjct: 550 LQRCCDEMDRAFGADAGYAQSRKTCAIGALELRVLRRGAFQEVLRHYVAGGSSAGQFKMP 609

Query: 601 RCT--TNQVLLTILNLSTVKAFHSTAY 608
           RC   +N  +L +L  +T+  F STAY
Sbjct: 610 RCVAPSNAGVLRVLKDNTINIFFSTAY 610

BLAST of Carg03169 vs. Swiss-Prot
Match: sp|Q9FZ87|GH317_ARATH (Indole-3-acetic acid-amido synthetase GH3.17 OS=Arabidopsis thaliana OX=3702 GN=GH3.17 PE=1 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 9.1e-102
Identity = 215/606 (35.48%), Postives = 342/606 (56.44%), Query Frame = 0

Query: 13  ERVSSEAGVAQLRTLREILRQNCGVEYLNQWL-GDVNIHQIPDDFALESLFTSLIPLSSH 72
           E +++ A   Q + L +IL QN G +YL  +L G+ + +Q          F + +P+ ++
Sbjct: 18  EDLTTNAEAIQQQVLHQILSQNSGTQYLRAFLDGEADKNQ--------QSFKNKVPVVNY 77

Query: 73  ADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRIAAA 132
            D +PF+QRIADG+SS +++ QPI  L  SSGT+ G+ K +P T    +     + +   
Sbjct: 78  DDVKPFIQRIADGESSDIVSAQPITELLTSSGTSAGKPKLMPSTAEELERKTFFYSMLVP 137

Query: 133 YRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQF 192
             ++Y       +GL         + G+ +  ++   + KT  G+      T YY S+ F
Sbjct: 138 IMNKY------VDGL---------DEGKGMYLLFIKPEIKTPSGLMARPVLTSYYKSQHF 197

Query: 193 KIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELE 252
           + +     +   SP + I   D KQS YC LL GL     V  V + FA   ++A   LE
Sbjct: 198 RNRPFNKYNVYTSPDQTILCQDSKQSMYCQLLCGLVQRSHVLRVGAVFASAFLRAVKFLE 257

Query: 253 DCWPQICHDITHATLCSRITLPEIRKAVLNIIS-PNPILGAKIQAVCEELERQDWLDLVP 312
           D + ++C DI   T+ S IT    R +VL+I++ PN  L  +I++ C E   + W  ++ 
Sbjct: 258 DHYKELCADIRTGTVTSWITDSSCRDSVLSILNGPNQELADEIESECAE---KSWEGILR 317

Query: 313 KLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTF 372
           ++WP  KYV  I+TGSM  Y+  L  Y+G LPLVS  Y S+E + G+N++P   P +V++
Sbjct: 318 RIWPKAKYVEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINLNPLCDPADVSY 377

Query: 373 AVVPTFSYFEFIPL-FRQHQQ-HDCGSSSVAVFDDFL-EGQPLPLSQVKIGQQYELVLTT 432
            ++P  +YFEF+P+  + H++ H    S+    DD L E   + L  V++GQ YE+V+TT
Sbjct: 378 TLLPNMAYFEFLPVDDKSHEEIHFATHSNTDDDDDALKEDLIVNLVNVEVGQYYEIVITT 437

Query: 433 FTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD-LQLVVERGSQLLA 492
           FTGLYR R+GD+++V GFHNK P+  F+ RR +VL+++ DK +E+D L  V +     L 
Sbjct: 438 FTGLYRYRVGDILKVTGFHNKAPQFRFVQRRNVVLSIDTDKTSEEDLLNAVTQAKLNHLQ 497

Query: 493 DQSGAELVDFTSHADLTNQPGHYVIFWEVKGH-------VEDAVLGHCCSEMDAAFVDYG 552
             S   L ++TS+AD ++ PGHYV+FWE+K         ++D  +  CCSE++   +DY 
Sbjct: 498 HPSSLLLTEYTSYADTSSIPGHYVLFWELKPRHSNDPPKLDDKTMEDCCSEVEDC-LDYV 557

Query: 553 YVVSR-RSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLST 605
           Y   R R  +IGPLE+R+V  G+F+ +M+  +  G++L+Q+KTPRC  +   L IL+   
Sbjct: 558 YRRCRNRDKSIGPLEIRVVSLGTFDSLMDFCVSQGSSLNQYKTPRCVKSGGALEILDSRV 596

BLAST of Carg03169 vs. TrEMBL
Match: tr|A0A1S3BIL6|A0A1S3BIL6_CUCME (jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490483 PE=4 SV=1)

HSP 1 Score: 1008.1 bits (2605), Expect = 8.7e-291
Identity = 493/605 (81.49%), Postives = 540/605 (89.26%), Query Frame = 0

Query: 3   EEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 62
           EE ED++DWFE+VSSEA VAQ RTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L+S+F
Sbjct: 8   EEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLQSIF 67

Query: 63  TSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTT 122
           TSLIPLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTPHSA TT
Sbjct: 68  TSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAHTT 127

Query: 123 LLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTAT 182
           LLIFR+AAAYRS               RVYPTREGG+ILEFIYSSKQTKTKGGITTGTAT
Sbjct: 128 LLIFRLAAAYRS---------------RVYPTREGGKILEFIYSSKQTKTKGGITTGTAT 187

Query: 183 THYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYT 242
           THYYASE+FKIKQLKTKSFTCSPQEVISG DYKQSTYCHLLLGL +S++VEFVTSTFAYT
Sbjct: 188 THYYASEEFKIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYT 247

Query: 243 IVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELER 302
           IVQAFN+LE+ W +I HDI+HATL SRI +PEIRKAVL ++SP P LG KI+ VCEEL++
Sbjct: 248 IVQAFNQLEESWEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKK 307

Query: 303 QDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPC 362
           + WL L+PKLWPNCKYVYSIMTGSMQPYLKKLR Y G LPLVSGDYGSTESWIGVNVDP 
Sbjct: 308 EGWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPY 367

Query: 363 LPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYE 422
           LPPENVTFAV+PTFSYFEFIPLF+QH      SS+     DFLEGQP+ LS+VKIGQQYE
Sbjct: 368 LPPENVTFAVIPTFSYFEFIPLFKQHA---TTSSTDTAIHDFLEGQPIALSEVKIGQQYE 427

Query: 423 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGS 482
           LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGS
Sbjct: 428 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGS 487

Query: 483 QLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVV 542
           QL++  SGAELVDFTS+A+L+NQPGHYVIFWEVKG V D VLG CCSEMDAAFVDYGYVV
Sbjct: 488 QLMSQLSGAELVDFTSYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVV 547

Query: 543 SRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAF 602
           SR++N+IGPLELRIVERGSFNKI+E+YIGNGAALSQFKTPRCTTN  LL ILNLST+++F
Sbjct: 548 SRKANSIGPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSF 594

Query: 603 HSTAY 608
            STAY
Sbjct: 608 FSTAY 594

BLAST of Carg03169 vs. TrEMBL
Match: tr|A0A0A0KE57|A0A0A0KE57_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G124120 PE=4 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 9.9e-287
Identity = 486/605 (80.33%), Postives = 535/605 (88.43%), Query Frame = 0

Query: 3   EEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 62
           E  ED++ WFE+VSSEA +AQ RTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L S+F
Sbjct: 2   EGEEDIIGWFEQVSSEAALAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLHSIF 61

Query: 63  TSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTT 122
           TS IPLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQKYVPFTPHSAQTT
Sbjct: 62  TSSIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAQTT 121

Query: 123 LLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTAT 182
           LLIFR+AAAYRS               RVYP R+GG+ILEFIYSSKQTKTKGGITTGTAT
Sbjct: 122 LLIFRLAAAYRS---------------RVYPIRDGGKILEFIYSSKQTKTKGGITTGTAT 181

Query: 183 THYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYT 242
           THYYASE+FKIKQLKTKSFTCSPQEVI+G DYKQSTYCHLLLGL +S++VEFVTSTFAYT
Sbjct: 182 THYYASEEFKIKQLKTKSFTCSPQEVITGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYT 241

Query: 243 IVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELER 302
           IVQAFN+LE+ W ++ HD++HATL SRI +PEIRKAVL ++SP P LG KI+ VCEEL +
Sbjct: 242 IVQAFNQLEESWEEMIHDLSHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELMK 301

Query: 303 QDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPC 362
           + WL L+PKLWPNCKYVYSIMTGSMQPYLKKLR Y G LPLVSGDYGSTESWIGVNVDP 
Sbjct: 302 EGWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPY 361

Query: 363 LPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYE 422
           LPPENVTFAV+PTFSYFEFIPLF+QH      SS+     DFLEGQP+ LS+VKIGQQYE
Sbjct: 362 LPPENVTFAVIPTFSYFEFIPLFKQHA---TTSSADTAIHDFLEGQPIALSEVKIGQQYE 421

Query: 423 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGS 482
           LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGS
Sbjct: 422 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGS 481

Query: 483 QLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVV 542
           QL++  SGAELVDFTS+A+L+NQPGHYVIFWE+KG V D VLG CCSEMDAAFVDYGYVV
Sbjct: 482 QLMSQLSGAELVDFTSYAELSNQPGHYVIFWELKGDVNDKVLGMCCSEMDAAFVDYGYVV 541

Query: 543 SRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAF 602
           SR++N+IGPLELRIVERGSFNKI+EHYIGNGAALSQFKTPRCTTN  LL ILNLST+K+F
Sbjct: 542 SRKANSIGPLELRIVERGSFNKILEHYIGNGAALSQFKTPRCTTNHFLLNILNLSTLKSF 588

Query: 603 HSTAY 608
            STAY
Sbjct: 602 FSTAY 588

BLAST of Carg03169 vs. TrEMBL
Match: tr|A0A1S4DX22|A0A1S4DX22_CUCME (jasmonic acid-amido synthetase JAR1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490483 PE=4 SV=1)

HSP 1 Score: 953.0 bits (2462), Expect = 3.3e-274
Identity = 470/605 (77.69%), Postives = 516/605 (85.29%), Query Frame = 0

Query: 3   EEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLF 62
           EE ED++DWFE+VSSEA VAQ RTLR+IL+QN GVEYL +W+GDVNIH+IPDDF L+S+F
Sbjct: 8   EEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLQSIF 67

Query: 63  TSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTT 122
           TSLIPLSSHA FEPFLQRIADGDSSPLLTQQPI TLSLSSGTTEGRQK            
Sbjct: 68  TSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQK------------ 127

Query: 123 LLIFRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTAT 182
                                       VYPTREGG+ILEFIYSSKQTKTKGGITTGTAT
Sbjct: 128 ----------------------------VYPTREGGKILEFIYSSKQTKTKGGITTGTAT 187

Query: 183 THYYASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYT 242
           THYYASE+FKIKQLKTKSFTCSPQEVISG DYKQSTYCHLLLGL +S++VEFVTSTFAYT
Sbjct: 188 THYYASEEFKIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYT 247

Query: 243 IVQAFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELER 302
           IVQAFN+LE+ W +I HDI+HATL SRI +PEIRKAVL ++SP P LG KI+ VCEEL++
Sbjct: 248 IVQAFNQLEESWEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKK 307

Query: 303 QDWLDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPC 362
           + WL L+PKLWPNCKYVYSIMTGSMQPYLKKLR Y G LPLVSGDYGSTESWIGVNVDP 
Sbjct: 308 EGWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPY 367

Query: 363 LPPENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYE 422
           LPPENVTFAV+PTFSYFEFIPLF+QH      SS+     DFLEGQP+ LS+VKIGQQYE
Sbjct: 368 LPPENVTFAVIPTFSYFEFIPLFKQHA---TTSSTDTAIHDFLEGQPIALSEVKIGQQYE 427

Query: 423 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGS 482
           LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKD+QL VERGS
Sbjct: 428 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGS 487

Query: 483 QLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVV 542
           QL++  SGAELVDFTS+A+L+NQPGHYVIFWEVKG V D VLG CCSEMDAAFVDYGYVV
Sbjct: 488 QLMSQLSGAELVDFTSYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVV 547

Query: 543 SRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAF 602
           SR++N+IGPLELRIVERGSFNKI+E+YIGNGAALSQFKTPRCTTN  LL ILNLST+++F
Sbjct: 548 SRKANSIGPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSF 569

Query: 603 HSTAY 608
            STAY
Sbjct: 608 FSTAY 569

BLAST of Carg03169 vs. TrEMBL
Match: tr|M5XH12|M5XH12_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G233900 PE=4 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 2.7e-260
Identity = 439/602 (72.92%), Postives = 511/602 (84.88%), Query Frame = 0

Query: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65
           ED++ WFE V+ +AG+ Q +TLR+IL QNC VEYL QWLGD+ I ++ D F LESL+TSL
Sbjct: 17  EDIIGWFEDVTEKAGLVQTQTLRKILEQNCSVEYLRQWLGDIKIQEV-DGFVLESLYTSL 76

Query: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125
           +PL+SHAD EP++ +IA+GD+ P+LTQQP+ TLSLSSGTT+GRQKYVPFT HSAQTTL I
Sbjct: 77  VPLASHADLEPYIHKIANGDTGPILTQQPMTTLSLSSGTTDGRQKYVPFTRHSAQTTLQI 136

Query: 126 FRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHY 185
           FR+AAAYRS               RVYPTREGGRILEFIYSS+Q KTKGG+T GTATTHY
Sbjct: 137 FRLAAAYRS---------------RVYPTREGGRILEFIYSSRQFKTKGGLTAGTATTHY 196

Query: 186 YASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQ 245
           YAS++FKIKQ KTKSFTCSP+EVI+ GD KQSTYCHLLLGLFFSDQVEF+TSTFAY+IVQ
Sbjct: 197 YASQEFKIKQEKTKSFTCSPEEVIASGDSKQSTYCHLLLGLFFSDQVEFITSTFAYSIVQ 256

Query: 246 AFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDW 305
           AF   E+ W  +C+DI   TL  RI LP++RKAVL+IISPNP L +KI+A C+ LE  DW
Sbjct: 257 AFITFEELWKDLCNDIRDGTLSKRINLPKVRKAVLDIISPNPTLASKIEASCKGLEDLDW 316

Query: 306 LDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPP 365
             L+PKLWPN KYVYSIMTGSMQPYLKKLRR+AG +PLVS +YGSTESWIGVNVDPCLPP
Sbjct: 317 FGLIPKLWPNAKYVYSIMTGSMQPYLKKLRRFAGDVPLVSAEYGSTESWIGVNVDPCLPP 376

Query: 366 ENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYELVL 425
           E+VTFAVVPTFSYFEFIPL R  Q  DC        DDF+E +P+PLSQVK+GQQYE+VL
Sbjct: 377 EDVTFAVVPTFSYFEFIPLHRHKQ--DCN----LAIDDFIEDKPVPLSQVKVGQQYEIVL 436

Query: 426 TTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLL 485
           TTFTGLYR RLGDVVEVAGFHN TPKL+FICRRKL+LTVNIDKNTEKDLQ+VVERGSQLL
Sbjct: 437 TTFTGLYRYRLGDVVEVAGFHNGTPKLDFICRRKLILTVNIDKNTEKDLQIVVERGSQLL 496

Query: 486 ADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRR 545
           + +S AELVDFTSHADL  QPGHY+I+WE+KG VE+ VLG CC EMDA+FVD+GYVVSRR
Sbjct: 497 S-KSKAELVDFTSHADLLTQPGHYIIYWEIKGEVEERVLGECCREMDASFVDHGYVVSRR 556

Query: 546 SNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHST 605
           SN+IGPLELRIVE+G+F KI+EH+I NG+ALSQFKTPRCT+N+V+L+ILNL T+K  +ST
Sbjct: 557 SNSIGPLELRIVEKGTFKKILEHFIVNGSALSQFKTPRCTSNKVILSILNLCTIKRVYST 595

Query: 606 AY 608
           AY
Sbjct: 617 AY 595

BLAST of Carg03169 vs. TrEMBL
Match: tr|A0A2P6S322|A0A2P6S322_ROSCH (Putative GH3 family protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0162261 PE=4 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 5.7e-258
Identity = 441/602 (73.26%), Postives = 511/602 (84.88%), Query Frame = 0

Query: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65
           +D++ WFE VS  A + Q +TLR IL  N GVEYL +WLGDV I ++ D+ ALESL+TSL
Sbjct: 14  DDIIQWFEDVSENAELVQTQTLRRILELNWGVEYLKKWLGDVEIDEV-DECALESLYTSL 73

Query: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125
           +PL SHAD EP++QRIA+GDS+PLLTQQP+ TLSLSSGTTEGRQKYVPFT HSAQTTL I
Sbjct: 74  VPLVSHADLEPYIQRIANGDSAPLLTQQPMTTLSLSSGTTEGRQKYVPFTRHSAQTTLQI 133

Query: 126 FRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHY 185
           FR+AAAYRS               RVYPTREGG+ILEFIYSSKQ KT+GG+T GTATTHY
Sbjct: 134 FRLAAAYRS---------------RVYPTREGGKILEFIYSSKQFKTRGGLTAGTATTHY 193

Query: 186 YASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQ 245
           YAS++FKIKQ  TKSFTCSP+EVIS GD KQ+TYCHLLLGLFFSD VEF+TSTFAY+IVQ
Sbjct: 194 YASQEFKIKQETTKSFTCSPEEVISSGDNKQATYCHLLLGLFFSDHVEFITSTFAYSIVQ 253

Query: 246 AFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDW 305
           +F   E+ W  +C DI   +L  RI +P++RK+VL+IISPNP L +KI+A CEELE  DW
Sbjct: 254 SFVTFEELWRDLCIDIRDGSLSKRINVPKVRKSVLDIISPNPKLASKIEAACEELEDLDW 313

Query: 306 LDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPP 365
             LVPKLWPN KYVYSIMTGSMQPYLKKLRRYA  +PLVS +YGSTESWIGVNVDPCLPP
Sbjct: 314 FGLVPKLWPNAKYVYSIMTGSMQPYLKKLRRYAADVPLVSAEYGSTESWIGVNVDPCLPP 373

Query: 366 ENVTFAVVPTFSYFEFIPLFRQHQQHDCGSSSVAVFDDFLEGQPLPLSQVKIGQQYELVL 425
           E+VTFAVVPTFSYFEFIPL+R  Q  DC   ++AV DDF+E +P+PLSQVK+GQQYE+VL
Sbjct: 374 EDVTFAVVPTFSYFEFIPLYRHEQ--DC---NLAV-DDFIEDKPVPLSQVKVGQQYEIVL 433

Query: 426 TTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLL 485
           TTFTGLYR RLGDVVEVAGFH  TPKLNFICRRKL+LTVNIDKNTEKDLQ+VVERGSQLL
Sbjct: 434 TTFTGLYRYRLGDVVEVAGFHRGTPKLNFICRRKLILTVNIDKNTEKDLQIVVERGSQLL 493

Query: 486 ADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRR 545
           + +S AEL+DFTSHAD+ NQPGHY+I+WE+KG VED VLG CC EMDA+FVD+GYVVSRR
Sbjct: 494 S-KSRAELIDFTSHADVVNQPGHYIIYWEIKGDVEDMVLGECCREMDASFVDHGYVVSRR 553

Query: 546 SNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHST 605
           SN+IGPLELRIVE+G+F KI+E++IGNG+ALSQFKTPRCT+N VLL+ILNL T+K  +ST
Sbjct: 554 SNSIGPLELRIVEKGTFKKILEYFIGNGSALSQFKTPRCTSNNVLLSILNLCTIKRVYST 592

Query: 606 AY 608
           AY
Sbjct: 614 AY 592

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023512179.10.0e+0096.88indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo][more]
XP_022943441.10.0e+0096.88indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata][more]
XP_022986272.10.0e+0096.05indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima][more]
XP_008448228.11.3e-29081.49PREDICTED: jasmonic acid-amido synthetase JAR1 isoform X1 [Cucumis melo][more]
XP_004140048.11.5e-28680.33PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.5 [Cucumis sativus... [more]
Match NameE-valueIdentityDescription
AT4G03400.14.3e-23565.67Auxin-responsive GH3 family protein[more]
AT2G46370.47.0e-15344.95Auxin-responsive GH3 family protein[more]
AT1G28130.15.1e-10335.48Auxin-responsive GH3 family protein[more]
AT4G37390.12.0e-9933.06Auxin-responsive GH3 family protein[more]
AT2G47750.17.6e-9934.28putative indole-3-acetic acid-amido synthetase GH3.9[more]
Match NameE-valueIdentityDescription
sp|Q9ZNS2|GH310_ARATH7.7e-23465.67Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=... [more]
sp|Q6I581|GH35_ORYSJ1.3e-15947.36Jasmonic acid-amido synthetase JAR1 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
sp|Q9SKE2|JAR1_ARATH1.3e-15144.95Jasmonic acid-amido synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1... [more]
sp|Q53P49|GH312_ORYSJ1.4e-14242.11Probable indole-3-acetic acid-amido synthetase GH3.12 OS=Oryza sativa subsp. jap... [more]
sp|Q9FZ87|GH317_ARATH9.1e-10235.48Indole-3-acetic acid-amido synthetase GH3.17 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
tr|A0A1S3BIL6|A0A1S3BIL6_CUCME8.7e-29181.49jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103... [more]
tr|A0A0A0KE57|A0A0A0KE57_CUCSA9.9e-28780.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G124120 PE=4 SV=1[more]
tr|A0A1S4DX22|A0A1S4DX22_CUCME3.3e-27477.69jasmonic acid-amido synthetase JAR1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103... [more]
tr|M5XH12|M5XH12_PRUPE2.7e-26072.92Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G233900 PE=4 SV=1[more]
tr|A0A2P6S322|A0A2P6S322_ROSCH5.7e-25873.26Putative GH3 family protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0162261 ... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004993GH3
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg03169-RACarg03169-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 11..587
e-value: 1.8E-174
score: 581.4
IPR004993GH3 familyPANTHERPTHR31901FAMILY NOT NAMEDcoord: 6..607
NoneNo IPR availablePANTHERPTHR31901:SF10AUXIN-RESPONSIVE GH3 FAMILY PROTEINcoord: 6..607