Carg01725 (gene) Silver-seed gourd

BLAST of Carg01725 vs. NCBI nr
Match: XP_022999568.1 (probable pectate lyase P59 [Cucurbita maxima])

HSP 1 Score: 838.2 bits (2164), Expect = 1.3e-239
Identity = 433/437 (99.08%), Postives = 436/437 (99.77%), Query Frame = 0

Query: 1   MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60
           MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN
Sbjct: 1   MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60

Query: 61  AEVEQELSGSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIG 120
           AEVEQELSGSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGW+TIG
Sbjct: 61  AEVEQELSGSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120

Query: 121 GKLGPFYVVNDSSDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180
           GKLGPFYVVNDSSDSDI EPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID
Sbjct: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180

Query: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240
           ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI
Sbjct: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240

Query: 241 FGSSNIWXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQV 300
           FGSSNIWXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQV
Sbjct: 241 FGSSNIWXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQV 300

Query: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360
           TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN
Sbjct: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360

Query: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420
           AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFS+MDMISY+EGTYVT
Sbjct: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSRMDMISYKEGTYVT 420

Query: 421 RMTRMSGALPCFIGRPC 438
           RMTRMSGALPCFIGRPC
Sbjct: 421 RMTRMSGALPCFIGRPC 437

BLAST of Carg01725 vs. NCBI nr
Match: XP_022957045.1 (probable pectate lyase P59 [Cucurbita moschata])

HSP 1 Score: 836.3 bits (2159), Expect = 4.9e-239
Identity = 433/437 (99.08%), Postives = 434/437 (99.31%), Query Frame = 0

Query: 1   MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60
           MVAPTSRAMLGVLLTLSLVATAYAD GNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN
Sbjct: 1   MVAPTSRAMLGVLLTLSLVATAYADTGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60

Query: 61  AEVEQELSGSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIG 120
           AEVEQELSGSNDTRRNLKRYTGPC ATNPIDRCWRCDPNWAKNRKKLADCVLGFGW+TIG
Sbjct: 61  AEVEQELSGSNDTRRNLKRYTGPCRATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120

Query: 121 GKLGPFYVVNDSSDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180
           GKLGPFYVVNDSSDSDI EPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID
Sbjct: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180

Query: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240
           ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI
Sbjct: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240

Query: 241 FGSSNIWXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQV 300
           FGSSNIWXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQV
Sbjct: 241 FGSSNIWXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQV 300

Query: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360
           TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN
Sbjct: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360

Query: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420
           AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT
Sbjct: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420

Query: 421 RMTRMSGALPCFIGRPC 438
           RMTRMSGALPCFIGRPC
Sbjct: 421 RMTRMSGALPCFIGRPC 437

BLAST of Carg01725 vs. NCBI nr
Match: XP_023537906.1 (probable pectate lyase P59 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 833.9 bits (2153), Expect = 2.4e-238
Identity = 432/437 (98.86%), Postives = 434/437 (99.31%), Query Frame = 0

Query: 1   MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60
           MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN
Sbjct: 1   MVAPTSRAMLGVLLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILN 60

Query: 61  AEVEQELSGSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIG 120
           AEVEQELSGSNDTRRNLKRY GPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGW+TIG
Sbjct: 61  AEVEQELSGSNDTRRNLKRYKGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWKTIG 120

Query: 121 GKLGPFYVVNDSSDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180
           GKLGPFYVVNDSSDSDI EPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID
Sbjct: 121 GKLGPFYVVNDSSDSDIMEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTID 180

Query: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISI 240
           ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRD+VDHVGLRTMSDGDGISI
Sbjct: 181 ARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDAVDHVGLRTMSDGDGISI 240

Query: 241 FGSSNIWXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQV 300
           FGSSNIWXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQV
Sbjct: 241 FGSSNIWXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQV 300

Query: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360
           TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN
Sbjct: 301 TVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVN 360

Query: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420
           AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT
Sbjct: 361 AKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVT 420

Query: 421 RMTRMSGALPCFIGRPC 438
           RMTRMSGAL CFIGRPC
Sbjct: 421 RMTRMSGALACFIGRPC 437

BLAST of Carg01725 vs. NCBI nr
Match: XP_008446316.1 (PREDICTED: probable pectate lyase P59 [Cucumis melo])

HSP 1 Score: 740.0 bits (1909), Expect = 4.8e-210
Identity = 378/431 (87.70%), Postives = 405/431 (93.97%), Query Frame = 0

Query: 8   AMLGVLLTLSLVA-TAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQE 67
           A L +LLTLSL+A TA+ADIG+FDDYWK+RAA+AE+AAA A++PNPE + D LNAEV Q 
Sbjct: 2   ARLSLLLTLSLLATTAFADIGDFDDYWKARAAEAEQAAADAYEPNPETIADSLNAEVGQA 61

Query: 68  LSGSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIGGKLGPF 127
           LSGSN TRRNLK+YTGPC ATNPIDRCWRCDPNWA+NRKKLADCVLGFG +TIGGKLGP+
Sbjct: 62  LSGSNSTRRNLKKYTGPCRATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTIGGKLGPY 121

Query: 128 YVVNDSSDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANV 187
           YVVNDSSDSD+  PKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIV+SDKTIDARGANV
Sbjct: 122 YVVNDSSDSDLMNPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVTSDKTIDARGANV 181

Query: 188 QIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNI 247
           QIAYGAGITLQYV+NVIIHGLRIHHIV+G+GGMIRD+VDHVGLRTMSDGDGISIFGSSN+
Sbjct: 182 QIAYGAGITLQYVRNVIIHGLRIHHIVVGNGGMIRDAVDHVGLRTMSDGDGISIFGSSNV 241

Query: 248 WXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNH 307
           WXXXXXXXXXXXXXXXXXXXXXXXXX   HFTHHN+VMLFGASDGYSNDQIMQVTVAFNH
Sbjct: 242 WXXXXXXXXXXXXXXXXXXXXXXXXXSNCHFTHHNEVMLFGASDGYSNDQIMQVTVAFNH 301

Query: 308 FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTK 367
           FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHP I+SQGNRFIAPPNV AKEVTK
Sbjct: 302 FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVTK 361

Query: 368 REYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMS 427
           REYSPE VWK+WTWRSEGDLMMNGAFFVTSGDQRK+RPFS  DMISY+ GTYV RMTR S
Sbjct: 362 REYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQRKRRPFSSKDMISYKPGTYVKRMTRFS 421

Query: 428 GALPCFIGRPC 438
           GAL CF+GRPC
Sbjct: 422 GALACFVGRPC 432

BLAST of Carg01725 vs. NCBI nr
Match: XP_004135462.1 (PREDICTED: probable pectate lyase P59 [Cucumis sativus] >KGN51889.1 hypothetical protein Csa_5G604340 [Cucumis sativus])

HSP 1 Score: 720.7 bits (1859), Expect = 3.0e-204
Identity = 364/429 (84.85%), Postives = 397/429 (92.54%), Query Frame = 0

Query: 10  LGVLLTLSLVA-TAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELS 69
           L +LLTLSL+A TA+ADIG+FDDYWK+RAA AEKAAA A+DP+PE V + +NAE+    S
Sbjct: 4   LSLLLTLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYDPDPETVANSVNAEIAHASS 63

Query: 70  GSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIGGKLGPFYV 129
           G N TRRNLK+Y GPCLATNPIDRCWRCDPNWA+NRKKLADCVLGFG +T GGKLGP+YV
Sbjct: 64  GRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGPYYV 123

Query: 130 VNDSSDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQI 189
           VNDSSDSD+  PKPGTLRHAVIQKGPLWI FSTNMAIRL+QELI++SDKTIDARGANVQI
Sbjct: 124 VNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGANVQI 183

Query: 190 AYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWX 249
           AYGAGITLQY++NVIIHGLRIHHIV+GSGGMIRD+VDHVGLRTMSDGDGISIFGSSN+W 
Sbjct: 184 AYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNVWI 243

Query: 250 XXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFG 309
           XXXXXXXXXXXXXXXXXXXXXXXX   HFTHHN+VMLFGASDGYS DQIMQ+TVAFNHFG
Sbjct: 244 XXXXXXXXXXXXXXXXXXXXXXXXSNCHFTHHNEVMLFGASDGYSQDQIMQITVAFNHFG 303

Query: 310 QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKRE 369
           QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHP I+SQGNRFIAPPNV AKEVTKRE
Sbjct: 304 QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVTKRE 363

Query: 370 YSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGA 429
           YSPE VWK+WTWRSEGDLMMNGAFFVTSGDQ K+RPFS+MDMISY+ GTYV RMTR +G+
Sbjct: 364 YSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVKRMTRFAGS 423

Query: 430 LPCFIGRPC 438
           L CF+GRPC
Sbjct: 424 LACFVGRPC 432

BLAST of Carg01725 vs. TAIR10
Match: AT3G01270.1 (Pectate lyase family protein)

HSP 1 Score: 477.6 bits (1228), Expect = 8.0e-135
Identity = 239/464 (51.51%), Postives = 303/464 (65.30%), Query Frame = 0

Query: 12  VLLTLSLVATAYADIGN-FDDYWKSRAAKAEKAAAKAFDPNP------------ENVTDI 71
           V+   SL+ T  A++ +  D+YW ++A +A K    A+ P+P            +N TD+
Sbjct: 12  VICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNSTDV 71

Query: 72  LNAEVEQEL------------SGSNDTRRNL------------KRYTGPCLATNPIDRCW 131
              E E+ +            S +N TRR+L             + TGPC A+NPID+CW
Sbjct: 72  EGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGXXXXXXXXXXSKLTGPCTASNPIDKCW 131

Query: 132 RCDPNWAKNRKKLADCVLGFGWRTIGGKLGPFYVVNDSSDSDITEPKPGTLRHAVIQKGP 191
           RC P+WA+ RKKL  CV GFG+RT GGK G  YVV    D D+  P+PGTLRHAVIQK P
Sbjct: 132 RCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEP 191

Query: 192 LWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVM 251
           LWI F  +M+IRL+QEL+++SDKTIDARGANV IAYGAGIT+QYV N+IIHGL +HHIV 
Sbjct: 192 LWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVK 251

Query: 252 GSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWXXXXXXXXXXXXXXXXXXXXXXXXXXX 311
            SGG+IRDS++H G R  +DGDGISIFG++NIW                           
Sbjct: 252 SSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISN 311

Query: 312 SHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWL 371
           SHFTHHNDVML GA +   +D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW 
Sbjct: 312 SHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWE 371

Query: 372 MYAIGGSQHPIIISQGNRFIAPPN-VNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFF 431
           +YAIGGSQ P I+S GNRFIAPP+  + +EVTKR+Y+ ES WK W WRSE D+ MN A+F
Sbjct: 372 LYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYF 431

Query: 432 VTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGALPCFIGRPC 438
             SG+   K   S+  MI  + G  V+++T+ +GAL C +G+ C
Sbjct: 432 RQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of Carg01725 vs. TAIR10
Match: AT1G14420.1 (Pectate lyase family protein)

HSP 1 Score: 474.6 bits (1220), Expect = 6.8e-134
Identity = 234/430 (54.42%), Postives = 285/430 (66.28%), Query Frame = 0

Query: 25  DIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGS---NDTRRNL---- 84
           ++  FD YW  R + A K    ++DP+P NVT+  N  V   +  S   NDTRR L    
Sbjct: 30  NVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRNDTRRELTQVR 89

Query: 85  -----KRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIGGKLGPFYVVNDS 144
                 + +G CLA NPID CWRCD NWA NRKKLADCVLGFG RT GGK GP YVV D+
Sbjct: 90  SGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGKDGPIYVVKDA 149

Query: 145 SDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGA 204
           SD+D+  PKPGTLRHAV + GPLWI F+ +M I+L QEL+++SDKTID RGA V I  GA
Sbjct: 150 SDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGRGARVYIMEGA 209

Query: 205 GITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWXXXXX 264
           G+TLQ+V NVIIH + + HIV G+GG+IRDS  H+GLRT SDGDGIS+FG++NIW     
Sbjct: 210 GLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFGATNIWIDHVS 269

Query: 265 XXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLV 324
                                 SHFT H +VMLFGA D +  D+ MQ+TVAFNHFG+ L 
Sbjct: 270 MTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITVAFNHFGKRLE 329

Query: 325 QRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSPE 384
           QRMPRCR+G  HVVNNDYTHW MYAIGG+ +P IISQGNRFIAPPN  AK++TKREY+P 
Sbjct: 330 QRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAKQITKREYTPY 389

Query: 385 SVWKTWTWRSEGDLMMNGAFFVTSG-----DQRKKRPFSKMDMISYREGTYVTRMTRMSG 438
             WK+W W+SEGD  +NGA+FV SG       + K P      I  + GT V ++T  +G
Sbjct: 390 GEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGTMVRKLTMDAG 449

BLAST of Carg01725 vs. TAIR10
Match: AT5G15110.1 (Pectate lyase family protein)

HSP 1 Score: 471.9 bits (1213), Expect = 4.4e-133
Identity = 235/455 (51.65%), Postives = 295/455 (64.84%), Query Frame = 0

Query: 18  LVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNP------------ENVTDILNAE--- 77
           L+ T   +I   D+YW  RA +A +   +A+  +P            +N TD+   E   
Sbjct: 18  LIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNSTDVTTPEEDG 77

Query: 78  -----------VEQELSGSNDTRRNLK--------RYTGPCLATNPIDRCWRCDPNWAKN 137
                      +E   S +N TRR+L+        +  GPC A+NPID+CWRC  +WAK 
Sbjct: 78  DAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSDWAKR 137

Query: 138 RKKLADCVLGFGWRTIGGKLGPFYVVNDSSDSDITEPKPGTLRHAVIQKGPLWITFSTNM 197
           RKKL  CV GFG RT GGK G  YVV  + D D+  PKPGTLRHAVIQK PLWI F  +M
Sbjct: 138 RKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEPLWIIFKNDM 197

Query: 198 AIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDS 257
           +IRL QEL+++S KTIDARGANV +A+GAGIT+Q+V+NV+IHGL IHHI   SGGMIRDS
Sbjct: 198 SIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIHHISESSGGMIRDS 257

Query: 258 VDHVGLRTMSDGDGISIFGSSNIWXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDV 317
           VDH G+RT +DGDG+SI+GSSNIW                           SHFTHHNDV
Sbjct: 258 VDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISNSHFTHHNDV 317

Query: 318 MLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQH 377
           ML GA +    D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW +YAIGGSQ 
Sbjct: 318 MLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWELYAIGGSQG 377

Query: 378 PIIISQGNRFIAPPN-VNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKK 437
           P I+S GNRFIAPP+  + +EVTKR+Y+ E  WK W WRS+ D+ MNGA+F  SG+ + K
Sbjct: 378 PTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYFRQSGNPQYK 437

BLAST of Carg01725 vs. TAIR10
Match: AT2G02720.1 (Pectate lyase family protein)

HSP 1 Score: 439.9 bits (1130), Expect = 1.9e-123
Identity = 229/455 (50.33%), Postives = 295/455 (64.84%), Query Frame = 0

Query: 1   MVAPTSRAMLGVLLTLSLVA-TAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDIL 60
           MV   S   + V L+L++V  +  A I  +D+YW  R   A +   +++DPNPENVTD  
Sbjct: 1   MVNLGSYVFVFVALSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHF 60

Query: 61  N--AEVEQELSG-SNDTRRNLK---------RYTGPCLATNPIDRCWRCDPNWAKNRKKL 120
           N  A +  E +G  N+TRR+L+         R  G   + N ID+CWR D NW KNRKKL
Sbjct: 61  NYHAALAMETTGIVNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKL 120

Query: 121 ADCVLGFGWRTIGGKLGPFYVVNDSSDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRL 180
           ADCVLGFG +T GGK GP YVV D SD+D+ +PKPGT+RHAV +  PLWI F+ +M I+L
Sbjct: 121 ADCVLGFGRKTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKL 180

Query: 181 TQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHV 240
            QELI+++DKTID RGA + I  GAG+TLQ+V+NVIIH + I  I  G+GG+I DS  H 
Sbjct: 181 QQELIITNDKTIDGRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHF 240

Query: 241 GLRTMSDGDGISIFGSSNIWXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFG 300
           GLRT+SDGDGI+IFG++N+W                           SHFT H++VMLFG
Sbjct: 241 GLRTVSDGDGINIFGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFG 300

Query: 301 ASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIII 360
            ++    D+ MQ+TVAFNHFG+ L QRMPR R+G  HVVNNDYTHW MYAIGG+ +P II
Sbjct: 301 GTNKDVIDKKMQITVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTII 360

Query: 361 SQGNRFIAPPNVNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSG-----DQRKK 420
           SQGNRFIAPP  ++K+VTKREY+P   WK+W W+SE D  +NGA+FV SG         K
Sbjct: 361 SQGNRFIAPPIEDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPK 420

Query: 421 RPFSKMDMISYREGTYVTRMTRMSGALPCFIGRPC 438
            P  +   I  + GT V R+T+ +G L C  G+ C
Sbjct: 421 NPIPRKFAIRPQPGTKVRRLTKDAGTLGCKPGKSC 455

BLAST of Carg01725 vs. TAIR10
Match: AT3G24670.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 408.7 bits (1049), Expect = 4.6e-114
Identity = 208/410 (50.73%), Postives = 256/410 (62.44%), Query Frame = 0

Query: 28  NFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGSNDTRRNLKRYTGPCLAT 87
           N D  W   A K           NPE V     A V+ ++  S + RR L  ++  C   
Sbjct: 50  NDDGAWNEHAVK-----------NPEEVA----AMVDMKIKNSTE-RRRLGFFS--CATG 109

Query: 88  NPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIGGKLGPFYVVNDSSDSDITEPKPGTLRH 147
           NPID CWRCD NW   RK+LA+C +GFG   IGG+ G +YVV D SD D   P+PGTLRH
Sbjct: 110 NPIDDCWRCDRNWHLRRKRLANCAIGFGRNAIGGRDGRYYVVTDPSDHDAVNPRPGTLRH 169

Query: 148 AVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGL 207
           AVIQ  PLWI F  +M I LTQELI++S KTID RG NV IA GA IT+QYV N+IIHG+
Sbjct: 170 AVIQDRPLWIVFKRDMVITLTQELIMNSFKTIDGRGVNVAIAGGACITIQYVTNIIIHGI 229

Query: 208 RIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWXXXXXXXXXXXXXXXXXXXX 267
            +H        M+R S  H G RTM+DGD ISIFGSS+IW                    
Sbjct: 230 NVHDCRRTGNAMVRSSPSHYGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLIDAIMGS 289

Query: 268 XXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVN 327
                  ++ THHN+VML G SD Y+ D++MQVT+A+NHFG+GL+QRMPRCR G+FHVVN
Sbjct: 290 TAITISNNYMTHHNEVMLMGHSDSYTRDKLMQVTIAYNHFGEGLIQRMPRCRHGYFHVVN 349

Query: 328 NDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSPESVWKTWTWRSEGDLM 387
           NDYTHW+MYAIGGS +P I SQGNRF+AP N  AKEVTKR  S +  WK W WRS+GDLM
Sbjct: 350 NDYTHWVMYAIGGSANPTINSQGNRFLAPGNPFAKEVTKRVGSWQGEWKQWNWRSQGDLM 409

Query: 388 MNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGALPCFIGRPC 438
           +NGA+F  SG       +++   +  +  + V+ +T  SGAL C IG  C
Sbjct: 410 LNGAYFTKSG-AAAPASYARASSLGAKPASVVSMLTYSSGALKCRIGMRC 440

BLAST of Carg01725 vs. Swiss-Prot
Match: sp|P15722|PLY59_SOLLC (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 6.7e-147
Identity = 253/432 (58.56%), Postives = 305/432 (70.60%), Query Frame = 0

Query: 17  SLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQEL--------S 76
           +++ +  A IG++D+ W+ RA +A++ A   ++P+PENVT   N ++   +        +
Sbjct: 19  TIIPSLMAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKLRDTMKELKKVKGT 78

Query: 77  GSNDTRRNL--KRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIGGKLGPF 136
            +N TRR L  K+YTGPC+ TNPID+CWRCDPNWA NRKKLADC +GFG + IGGK G F
Sbjct: 79  HNNSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMGFGSKAIGGKDGEF 138

Query: 137 YVVNDSSDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANV 196
           YVV D+SD D  +PKPGTLRHAVIQK PLWI F   M IRL QE+I+ SDKTIDARG NV
Sbjct: 139 YVVTDNSD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQSDKTIDARGVNV 198

Query: 197 QIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNI 256
            I  GAGITLQY++NVIIHGL IH IV G+GGM+RD+VDH+G+RT SDGDGISIFG+S I
Sbjct: 199 HITKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSDGDGISIFGASYI 258

Query: 257 WXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNH 316
           W                            HFT HN+VMLFGASD  S DQ+MQ+T+AFNH
Sbjct: 259 WIDHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSIDQVMQITLAFNH 318

Query: 317 FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTK 376
           FG+ L+QRMPRCRWG+ HVVNNDYTHW MYAIGGS HP II QGNRFIAPP++  K+VTK
Sbjct: 319 FGKRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFIAPPDIFKKQVTK 378

Query: 377 REYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKM-DMISYREGTYVTRMTRM 436
           REY+PESVW  WTWRSEG+L MNGA+F  SGD         + D IS      VT MTR 
Sbjct: 379 REYNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDLYDGISAAPAEDVTWMTRF 438

Query: 437 SGALPCFIGRPC 438
           +G L C  G+PC
Sbjct: 439 AGVLGCKPGKPC 449

BLAST of Carg01725 vs. Swiss-Prot
Match: sp|P40973|PLY_LILLO (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 3.1e-136
Identity = 242/426 (56.81%), Postives = 290/426 (68.08%), Query Frame = 0

Query: 13  LLTLSLVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGSND 72
           LL  +  A + A+I  FD+YW+ ++  A+  A KA+ P+PE VT+  N  V     G N 
Sbjct: 11  LLFFASAALSSANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFEG-NS 70

Query: 73  TRRNLK-RYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIGGKLGPFYVVND 132
           TRRNL+    G CLATNPIDRCWRC  NW+ NRK L  CV GFG +T GG  G  YVV D
Sbjct: 71  TRRNLRTNKLGQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIYVVTD 130

Query: 133 SSDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYG 192
            SD  +T+PK GTLR  VIQ  PLWI F  +M IRL QELI+++DKTID RGANVQIA G
Sbjct: 131 PSDDSLTDPKFGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQIAGG 190

Query: 193 AGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWXXXX 252
           A +T+Q+V NVIIHG+ IH I  G GG+IRDS  H G+RT SDGDGISI GSSNIW    
Sbjct: 191 AQLTVQFVHNVIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWIDHV 250

Query: 253 XXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGL 312
                                   H T H+DVML GASD Y+ D+IMQVTVAFNHFG+GL
Sbjct: 251 SLARCSDGLIDVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGRGL 310

Query: 313 VQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSP 372
           VQRMPRCR+GF HVVNNDYTHW+MYA+GGSQHP IISQGNR+IAP    AKEVTKR+Y+ 
Sbjct: 311 VQRMPRCRYGFVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKRDYAE 370

Query: 373 ESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGALPC 432
            + W  WTW+S+GDL ++GAFFV SG   + + +SK D+I  + GT+V R+TR SGAL C
Sbjct: 371 PAEWSKWTWKSQGDLFVSGAFFVESGGPFENK-YSKKDLIKAKPGTFVQRLTRFSGALNC 430

Query: 433 FIGRPC 438
                C
Sbjct: 431 KENMEC 434

BLAST of Carg01725 vs. Swiss-Prot
Match: sp|Q9SRH4|PLY7_ARATH (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 1.4e-133
Identity = 239/464 (51.51%), Postives = 303/464 (65.30%), Query Frame = 0

Query: 12  VLLTLSLVATAYADIGN-FDDYWKSRAAKAEKAAAKAFDPNP------------ENVTDI 71
           V+   SL+ T  A++ +  D+YW ++A +A K    A+ P+P            +N TD+
Sbjct: 12  VICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYDNSTDV 71

Query: 72  LNAEVEQEL------------SGSNDTRRNL------------KRYTGPCLATNPIDRCW 131
              E E+ +            S +N TRR+L             + TGPC A+NPID+CW
Sbjct: 72  EGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGXXXXXXXXXXSKLTGPCTASNPIDKCW 131

Query: 132 RCDPNWAKNRKKLADCVLGFGWRTIGGKLGPFYVVNDSSDSDITEPKPGTLRHAVIQKGP 191
           RC P+WA+ RKKL  CV GFG+RT GGK G  YVV    D D+  P+PGTLRHAVIQK P
Sbjct: 132 RCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEP 191

Query: 192 LWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVM 251
           LWI F  +M+IRL+QEL+++SDKTIDARGANV IAYGAGIT+QYV N+IIHGL +HHIV 
Sbjct: 192 LWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVK 251

Query: 252 GSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWXXXXXXXXXXXXXXXXXXXXXXXXXXX 311
            SGG+IRDS++H G R  +DGDGISIFG++NIW                           
Sbjct: 252 SSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISN 311

Query: 312 SHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWL 371
           SHFTHHNDVML GA +   +D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW 
Sbjct: 312 SHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWE 371

Query: 372 MYAIGGSQHPIIISQGNRFIAPPN-VNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFF 431
           +YAIGGSQ P I+S GNRFIAPP+  + +EVTKR+Y+ ES WK W WRSE D+ MN A+F
Sbjct: 372 LYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYF 431

Query: 432 VTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGALPCFIGRPC 438
             SG+   K   S+  MI  + G  V+++T+ +GAL C +G+ C
Sbjct: 432 RQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of Carg01725 vs. Swiss-Prot
Match: sp|Q9M9S2|PLY3_ARATH (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 1.2e-132
Identity = 234/430 (54.42%), Postives = 285/430 (66.28%), Query Frame = 0

Query: 25  DIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGS---NDTRRNL---- 84
           ++  FD YW  R + A K    ++DP+P NVT+  N  V   +  S   NDTRR L    
Sbjct: 30  NVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRNDTRRELTQVR 89

Query: 85  -----KRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIGGKLGPFYVVNDS 144
                 + +G CLA NPID CWRCD NWA NRKKLADCVLGFG RT GGK GP YVV D+
Sbjct: 90  SGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGKDGPIYVVKDA 149

Query: 145 SDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGA 204
           SD+D+  PKPGTLRHAV + GPLWI F+ +M I+L QEL+++SDKTID RGA V I  GA
Sbjct: 150 SDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGRGARVYIMEGA 209

Query: 205 GITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWXXXXX 264
           G+TLQ+V NVIIH + + HIV G+GG+IRDS  H+GLRT SDGDGIS+FG++NIW     
Sbjct: 210 GLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFGATNIWIDHVS 269

Query: 265 XXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLV 324
                                 SHFT H +VMLFGA D +  D+ MQ+TVAFNHFG+ L 
Sbjct: 270 MTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITVAFNHFGKRLE 329

Query: 325 QRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSPE 384
           QRMPRCR+G  HVVNNDYTHW MYAIGG+ +P IISQGNRFIAPPN  AK++TKREY+P 
Sbjct: 330 QRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAKQITKREYTPY 389

Query: 385 SVWKTWTWRSEGDLMMNGAFFVTSG-----DQRKKRPFSKMDMISYREGTYVTRMTRMSG 438
             WK+W W+SEGD  +NGA+FV SG       + K P      I  + GT V ++T  +G
Sbjct: 390 GEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGTMVRKLTMDAG 449

BLAST of Carg01725 vs. Swiss-Prot
Match: sp|Q9LFP5|PLY19_ARATH (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 6.1e-132
Identity = 236/455 (51.87%), Postives = 295/455 (64.84%), Query Frame = 0

Query: 18  LVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNP------------ENVTDILNAE--- 77
           L+ T   +I   D+YW  RA +A +   +A+  +P            +N TD+   E   
Sbjct: 18  LIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNSTDVTTPEEDG 77

Query: 78  -----------VEQELSGSNDTRRNLK--------RYTGPCLATNPIDRCWRCDPNWAKN 137
                      +E   S +N TRR+L+        +  GPC A+NPID+CWRC  +WAK 
Sbjct: 78  DAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSDWAKR 137

Query: 138 RKKLADCVLGFGWRTIGGKLGPFYVVNDSSDSDITEPKPGTLRHAVIQKGPLWITFSTNM 197
           RKKL  CV GFG RT GGK G  YVV  + D D+  PKPGTLRHAVIQK PLWI F  +M
Sbjct: 138 RKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEPLWIIFKNDM 197

Query: 198 AIRLTQELIVSSDKTIDARGANVQIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDS 257
           +IRL QEL+++S KTIDARGANV +A+GAGIT+Q+V+NVIIHGL IHHI   SGGMIRDS
Sbjct: 198 SIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVIIHGLHIHHISESSGGMIRDS 257

Query: 258 VDHVGLRTMSDGDGISIFGSSNIWXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDV 317
           VDH G+RT +DGDG+SI+GSSNIW                           SHFTHHNDV
Sbjct: 258 VDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITISNSHFTHHNDV 317

Query: 318 MLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQH 377
           ML GA +    D+ MQVTVA+NHFG+GLVQRMPR RWGF HVVNNDYTHW +YAIGGSQ 
Sbjct: 318 MLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWELYAIGGSQG 377

Query: 378 PIIISQGNRFIAPPN-VNAKEVTKREYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKK 437
           P I+S GNRFIAPP+  + +EVTKR+Y+ E  WK W WRS+ D+ MNGA+F  SG+ + K
Sbjct: 378 PTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAYFRQSGNPQYK 437

BLAST of Carg01725 vs. TrEMBL
Match: tr|A0A1S3BFK5|A0A1S3BFK5_CUCME (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103489090 PE=3 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 3.2e-210
Identity = 378/431 (87.70%), Postives = 405/431 (93.97%), Query Frame = 0

Query: 8   AMLGVLLTLSLVA-TAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQE 67
           A L +LLTLSL+A TA+ADIG+FDDYWK+RAA+AE+AAA A++PNPE + D LNAEV Q 
Sbjct: 2   ARLSLLLTLSLLATTAFADIGDFDDYWKARAAEAEQAAADAYEPNPETIADSLNAEVGQA 61

Query: 68  LSGSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIGGKLGPF 127
           LSGSN TRRNLK+YTGPC ATNPIDRCWRCDPNWA+NRKKLADCVLGFG +TIGGKLGP+
Sbjct: 62  LSGSNSTRRNLKKYTGPCRATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTIGGKLGPY 121

Query: 128 YVVNDSSDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANV 187
           YVVNDSSDSD+  PKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIV+SDKTIDARGANV
Sbjct: 122 YVVNDSSDSDLMNPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVTSDKTIDARGANV 181

Query: 188 QIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNI 247
           QIAYGAGITLQYV+NVIIHGLRIHHIV+G+GGMIRD+VDHVGLRTMSDGDGISIFGSSN+
Sbjct: 182 QIAYGAGITLQYVRNVIIHGLRIHHIVVGNGGMIRDAVDHVGLRTMSDGDGISIFGSSNV 241

Query: 248 WXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNH 307
           WXXXXXXXXXXXXXXXXXXXXXXXXX   HFTHHN+VMLFGASDGYSNDQIMQVTVAFNH
Sbjct: 242 WXXXXXXXXXXXXXXXXXXXXXXXXXSNCHFTHHNEVMLFGASDGYSNDQIMQVTVAFNH 301

Query: 308 FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTK 367
           FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHP I+SQGNRFIAPPNV AKEVTK
Sbjct: 302 FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVTK 361

Query: 368 REYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMS 427
           REYSPE VWK+WTWRSEGDLMMNGAFFVTSGDQRK+RPFS  DMISY+ GTYV RMTR S
Sbjct: 362 REYSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQRKRRPFSSKDMISYKPGTYVKRMTRFS 421

Query: 428 GALPCFIGRPC 438
           GAL CF+GRPC
Sbjct: 422 GALACFVGRPC 432

BLAST of Carg01725 vs. TrEMBL
Match: tr|A0A0A0KSL4|A0A0A0KSL4_CUCSA (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_5G604340 PE=3 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 2.0e-204
Identity = 364/429 (84.85%), Postives = 397/429 (92.54%), Query Frame = 0

Query: 10  LGVLLTLSLVA-TAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELS 69
           L +LLTLSL+A TA+ADIG+FDDYWK+RAA AEKAAA A+DP+PE V + +NAE+    S
Sbjct: 4   LSLLLTLSLLATTAFADIGDFDDYWKARAAAAEKAAADAYDPDPETVANSVNAEIAHASS 63

Query: 70  GSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIGGKLGPFYV 129
           G N TRRNLK+Y GPCLATNPIDRCWRCDPNWA+NRKKLADCVLGFG +T GGKLGP+YV
Sbjct: 64  GRNSTRRNLKKYAGPCLATNPIDRCWRCDPNWAQNRKKLADCVLGFGRKTTGGKLGPYYV 123

Query: 130 VNDSSDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQI 189
           VNDSSDSD+  PKPGTLRHAVIQKGPLWI FSTNMAIRL+QELI++SDKTIDARGANVQI
Sbjct: 124 VNDSSDSDLMNPKPGTLRHAVIQKGPLWIIFSTNMAIRLSQELIMTSDKTIDARGANVQI 183

Query: 190 AYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWX 249
           AYGAGITLQY++NVIIHGLRIHHIV+GSGGMIRD+VDHVGLRTMSDGDGISIFGSSN+W 
Sbjct: 184 AYGAGITLQYIRNVIIHGLRIHHIVVGSGGMIRDAVDHVGLRTMSDGDGISIFGSSNVWI 243

Query: 250 XXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFG 309
           XXXXXXXXXXXXXXXXXXXXXXXX   HFTHHN+VMLFGASDGYS DQIMQ+TVAFNHFG
Sbjct: 244 XXXXXXXXXXXXXXXXXXXXXXXXSNCHFTHHNEVMLFGASDGYSQDQIMQITVAFNHFG 303

Query: 310 QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKRE 369
           QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHP I+SQGNRFIAPPNV AKEVTKRE
Sbjct: 304 QGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNVYAKEVTKRE 363

Query: 370 YSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGA 429
           YSPE VWK+WTWRSEGDLMMNGAFFVTSGDQ K+RPFS+MDMISY+ GTYV RMTR +G+
Sbjct: 364 YSPEQVWKSWTWRSEGDLMMNGAFFVTSGDQSKRRPFSRMDMISYKPGTYVKRMTRFAGS 423

Query: 430 LPCFIGRPC 438
           L CF+GRPC
Sbjct: 424 LACFVGRPC 432

BLAST of Carg01725 vs. TrEMBL
Match: tr|A0A059BFL8|A0A059BFL8_EUCGR (Pectate lyase OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_G02541 PE=3 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 6.9e-173
Identity = 287/420 (68.33%), Postives = 335/420 (79.76%), Query Frame = 0

Query: 18  LVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGSNDTRRNL 77
           LV +  A+I  FD+YW  RA +A+K A KA+DP+PE VT + NA+V++ + G+N TRR L
Sbjct: 17  LVPSLQANIEEFDEYWAKRAEEAQKDALKAYDPHPETVTSLFNAKVDKAVRGNNSTRRQL 76

Query: 78  KRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIGGKLGPFYVVNDSSDSDI 137
           K+Y GPCLATNPIDRCWRC+ NWA+NRK+LA+CVLGFG +T GGK G FYVV DSSD+D+
Sbjct: 77  KKYKGPCLATNPIDRCWRCNRNWARNRKRLANCVLGFGRKTRGGKRGWFYVVTDSSDNDM 136

Query: 138 TEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITLQ 197
             PKPGTLRHAV+QKGPLWI F+ +M IRL QELI++SDKTIDARGANV IAYGAGITLQ
Sbjct: 137 VNPKPGTLRHAVVQKGPLWIIFAGSMVIRLNQELIMTSDKTIDARGANVHIAYGAGITLQ 196

Query: 198 YVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWXXXXXXXXXX 257
           +++NVIIHGL IH IV GSGG+IRD+ DHVGLRT SDGDGISIFGSS++W          
Sbjct: 197 FIRNVIIHGLHIHDIVRGSGGLIRDAHDHVGLRTASDGDGISIFGSSHLWIDHVSMSACS 256

Query: 258 XXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPR 317
                             HFTHHN+VMLFGASDGYS DQIMQ+TVAFNHFG+GLVQRMPR
Sbjct: 257 DGLIDAIQGSTAITISNCHFTHHNEVMLFGASDGYSQDQIMQITVAFNHFGRGLVQRMPR 316

Query: 318 CRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSPESVWKT 377
           CRWGFFHVVNNDYTHWLMYAIGGSQHP IISQGNRFIAPPN+ AKEVTKR+Y+ E  WK 
Sbjct: 317 CRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNIAAKEVTKRDYATEDQWKN 376

Query: 378 WTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGALPCFIGRPC 437
           W W+SEGDLMMNGAFFV SG+ R + PFS+ D+I  + GTYV R+TR +G L C +GRPC
Sbjct: 377 WLWKSEGDLMMNGAFFVNSGNPRARFPFSRRDVIKAKPGTYVRRLTRFAGKLGCRVGRPC 436

BLAST of Carg01725 vs. TrEMBL
Match: tr|A0A2P5FDL6|A0A2P5FDL6_9ROSA (Pectate lyase OS=Trema orientalis OX=63057 GN=TorPLL5 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 2.3e-168
Identity = 286/431 (66.36%), Postives = 332/431 (77.03%), Query Frame = 0

Query: 10  LGVLLTLS---LVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQE 69
           LG+L  +S   L+ +  A+I  FD+ W+ RA +A+KAA +A+DPNPE VT+ LN EV + 
Sbjct: 6   LGLLFFISFVILIPSRLANIAEFDESWQQRAEEAQKAALEAYDPNPEEVTENLNFEVSKV 65

Query: 70  LSGSNDTRRNLKRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIGGKLGPF 129
           + GSN TRRNL+RY GPCLATNPIDRCWRC  NWA NRK+LA CVLGFG +T GGK G +
Sbjct: 66  IEGSNSTRRNLRRYNGPCLATNPIDRCWRCRKNWASNRKRLAQCVLGFGRKTTGGKSGKY 125

Query: 130 YVVNDSSDSDITEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANV 189
           YVVND SD D+  PKPGTLRHAVIQK PLWITFS +M IRL+QEL+++S KTID RGANV
Sbjct: 126 YVVNDPSDHDVQNPKPGTLRHAVIQKQPLWITFSRSMVIRLSQELLITSHKTIDGRGANV 185

Query: 190 QIAYGAGITLQYVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNI 249
            IA GAGIT+Q+ +NVIIHGL IH IV   GG+IRDSVDH+G RT++DGDGISIFGS+N+
Sbjct: 186 HIANGAGITIQFARNVIIHGLHIHDIVSSGGGLIRDSVDHMGFRTVADGDGISIFGSTNV 245

Query: 250 WXXXXXXXXXXXXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNH 309
           W                           SHFTHHNDVMLFGASD +  D+IMQVTVAFNH
Sbjct: 246 WLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSFEGDKIMQVTVAFNH 305

Query: 310 FGQGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTK 369
           FG+GLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGS HP IISQGNRFIAPPN  AKEVTK
Sbjct: 306 FGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSTHPTIISQGNRFIAPPNNAAKEVTK 365

Query: 370 REYSPESVWKTWTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMS 429
           R+Y+ E  WK WTWRSEGDLMMNGAFFV SG+   KR FS+ DMI  + G++VTR+TR +
Sbjct: 366 RDYAAEWEWKKWTWRSEGDLMMNGAFFVQSGNPSNKRSFSRRDMIKAKPGSFVTRLTRFA 425

Query: 430 GALPCFIGRPC 438
           GAL C IG+ C
Sbjct: 426 GALNCKIGQKC 436

BLAST of Carg01725 vs. TrEMBL
Match: tr|A0A2I4DZW2|A0A2I4DZW2_9ROSI (Pectate lyase OS=Juglans regia OX=51240 GN=LOC108985018 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 2.3e-168
Identity = 279/420 (66.43%), Postives = 330/420 (78.57%), Query Frame = 0

Query: 18  LVATAYADIGNFDDYWKSRAAKAEKAAAKAFDPNPENVTDILNAEVEQELSGSNDTRRNL 77
           +V + +A+I  +D YWK R+ +A KA   A+DP+PE +TD  N+ V++ L+GSN TRRNL
Sbjct: 20  VVPSLHANISEWDGYWKHRSDEALKANLAAYDPHPEKITDDFNSHVDKVLTGSNSTRRNL 79

Query: 78  KRYTGPCLATNPIDRCWRCDPNWAKNRKKLADCVLGFGWRTIGGKLGPFYVVNDSSDSDI 137
           K+ TGPC ATNPIDRCWRCDP+W KNRKKLADCVLGFG  T+GGK GP+YVV DSSD D+
Sbjct: 80  KKNTGPCTATNPIDRCWRCDPDWVKNRKKLADCVLGFGRNTVGGKYGPYYVVTDSSDDDL 139

Query: 138 TEPKPGTLRHAVIQKGPLWITFSTNMAIRLTQELIVSSDKTIDARGANVQIAYGAGITLQ 197
             PKPGTLRHAVIQKGPLWI F+ +M IRL +ELI++SDKTIDARGANV IA GAGIT+Q
Sbjct: 140 VNPKPGTLRHAVIQKGPLWIIFAGSMIIRLHEELIMTSDKTIDARGANVHIANGAGITIQ 199

Query: 198 YVQNVIIHGLRIHHIVMGSGGMIRDSVDHVGLRTMSDGDGISIFGSSNIWXXXXXXXXXX 257
           +++NVIIH L IH+I  G+GG+I DSVDH G+RT SDGDGISIFGSSN+W          
Sbjct: 200 FIKNVIIHNLHIHNIYAGNGGLIADSVDHFGIRTPSDGDGISIFGSSNVWIDHVSMSQCK 259

Query: 258 XXXXXXXXXXXXXXXXXSHFTHHNDVMLFGASDGYSNDQIMQVTVAFNHFGQGLVQRMPR 317
                             HFT HN+VMLFGASD YS D+IMQ+TVAFNHFG+GLVQRMPR
Sbjct: 260 DGLIDAIMGSTGITISNCHFTDHNEVMLFGASDSYSGDEIMQITVAFNHFGRGLVQRMPR 319

Query: 318 CRWGFFHVVNNDYTHWLMYAIGGSQHPIIISQGNRFIAPPNVNAKEVTKREYSPESVWKT 377
           CRWGF HVVNNDYTHWLMYAIGGS HP IISQGNRFIAPP++ AKEVTKR+Y+ ES W +
Sbjct: 320 CRWGFVHVVNNDYTHWLMYAIGGSSHPTIISQGNRFIAPPDLTAKEVTKRDYATESEWSS 379

Query: 378 WTWRSEGDLMMNGAFFVTSGDQRKKRPFSKMDMISYREGTYVTRMTRMSGALPCFIGRPC 437
           WTWRSEGDLMMNGAFFV SG++ K + FS++DMI  + GTYV R+TR SG+L C  G+PC
Sbjct: 380 WTWRSEGDLMMNGAFFVQSGEKLKNKAFSRLDMIKSKSGTYVRRLTRHSGSLKCQPGKPC 439