CSPI07G22140 (gene) Wild cucumber (PI 183967)

NameCSPI07G22140
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionSubtilisin-like protease
LocationChr7 : 18793595 .. 18796536 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCCATTTCCAAATAAATTTTTACCAGAACCCCTTATCTGAAGAAATATCAGTAGAGGGAGCCAGAGAGTCCCAACACTCTGCTCACCTCTTCAAACTTTACTGCACTCTACGCGTTTTATTACTACATTTGTTGCCTCTCACGCACTCCCACTACACTACAACTCCACGTCCCTCACCTATCTCTCTCTCTACACCCACTTCCCTTTTTAAAGTTTTTCCTTTCACTGCCAGTGACAGACCATTGCCAAAACCCCCCCTTCACTTTACTTCTCTCTCATGGCTGTTCTTCTTCGAGCATTCTTTCTCTTCTCTCTCCTCATTCCATTTTCTTCGTCTTCTTCGATTGATGCTTCAAAGAAAACTTTCATTGTTCAGGTTCACAAAGATTCCAAGCCTTCTATTTTCCCAACCCACAAGAACTGGTATGAGTCCTCTTTAGCATCCATCTCCTCTGTCAATGATGTAGGAGCCATCATTCATACATATGAAACTCTTTTCCATGGCTTCTCTGCTAAGCTCTCACCTCTTGAAGTTGAGAAGCTTCAAACTCTCCCCCACGTCGCTTCCATCATTCCTGAACAGGTTCGTCATCCTCACACAACGCGTTCCCCTGAGTTTCTTGGCCTTAAGACATCTGATAGTGCTGGGTTGCTTAAAGAGTCCGACTTTGGATCTGACCTTGTAATTGGTGTTATTGATACTGGTATTTGGCCGGAGAGGCAGAGTTTTAACGACCGCGATCTGGGTCCTGTTCCTTCCAAATGGAAAGGTCAGTGTTTGGTGGCTAAAGACTTTCCAGCTACCTCTTGCAACCGTAAACTCATTGGGGCTCGATTTTTCTGTAGTGGGTACGAGGCTACCAATGGAAAGATGAATGAAACCACCGAGTATCGCTCGCCGCGAGATTCGGATGGCCATGGTACACATACAGCGTCCATTGCCGCAGGTCGTTATGTGTTTCCAGCTTCAACTTTGGGGTATGCTCGTGGTAAAGCTGCTGGAATGGCTCCTAAGGCCCGTCTTGCTGCTTATAAAGTGTGCTGGAACGCCGGCTGTTACGACTCCGACATTCTTGCTGCTTTTGATGCTGCCGTTTCCGACGGTGTTGATGTTGTATCACTCAGCGTCGGTGGTGTGGTTGTCCCGTACTATCTCGATGCCATCGCTATTGGGGCTTATCGAGCTGTGGCTGCCGGTGTTTTTGTCTCCGCATCGGCCGGTAATGGTGGTCCAGGTGGGCTCACTGTTACTAATGTAGCACCATGGGTTACAACAGTAGGAGCTGGAACCATGGATAGAGATTTTCCAGCTGATGTTAAGCTCGGAAATGGGAGGGTTGTACTTGGTACTAGTGTCTATGGCGGACCGGCTTTGATTCCGGGTCGTTTGTATCCTCTTATTTATGCAGGAACTGAAGGCGGTGATGGATATTCTTCGTCTCTATGCTTAGAAGGTTCGTTGAACCCCAATTTAGTGAAAGGGAAGATTGTACTTTGTGACAGGGGAATCAATTCAAGAGCTGCTAAAGGTGAGGTTGTGAAAAAGGCTGGAGGATTGGGAATGATTTTAGCAAATGGGGTCTTTGACGGTGAGGGTTTGGTAGCTGACTGCCACGTACTACCTGCAACCGCCGTCGGTGCTTCCGGCGGCGACGAGATTCGCAAATATATTGCAGAAGCAGCGAAGTCTCATTTACAGCCAACCGCTACAATTTTGTTCAAGGGAACTCGGCTTGGAGTTAGGCCAGCACCGGTTGTTGCTTCATTTTCAGCTCGAGGTCCAAATCCAGAGTCACCTGAAATTGTAAAGCCGGATGTGATTGCGCCTGGGTTGAACATTCTTGCCGCTTGGCCTGACAAAATTGGACCTTCTGGAATTCCCACAGATAAGCGTACCACTGAGTTCAACATACTTTCGGGCACGTCCATGGCCTGTCCTCATGTCTCTGGCTTAGCTGCCCTGCTGAAGGCTGCACACCCAGGATGGAGTCCAGCAGCTATAAAATCAGCCCTGATGACCACAGCTTATACTTTGGACAACCGGGGTGAGACGATGTTAGATGAATCTTCAGGCAATACATCCACGGTTTTGGACTTTGGAGCTGGCCATGTTCACCCACAAAAGGCAATGGACCCTGGTTTAATCTATGACCTGAATACATATGATTACGTTGATTTCTTGTGCAACTCCAATTACACTACTAAGAATATCCAAGTTATTACTGGGAAGATAGCAGATTGTAGTGGGGCGAAAAGGGCTGGACATACTGGGAATTTGAATTACCCTTCATTGGCCGTGGTGTTTCAACAATATGGTAAGCATAAGATGTCTACACATTTCATAAGAACTGTGACTAACGTTGGAGACGCCAATTCTATCTATAAGGTAACAATAAAGCCACCAAGTGGAATTTCAGTGACAGTTGAACCAGAAAAGCTGGCGTTTAGAAGGGTTGGGCAAAAATTGAGCTTCTTGGTTAGGGTCCAAGCAATGGCTGTTAGACTTTCCCCTGGAAGCTCTAGCATGAAGAGTGGTTCTATAATTTGGACTGATGGAAAGCACGAAGTCACTAGTCCTTTGGTTGTTACCATGCAGCAACCTCTGCAGTAGGATCATCATAATTTTGCTCTTTCCCAACTACGTAGTTTTTCTTCTCTGTATGTATAATGGAAATGCTTTTTCCTCCCCCTTCTCTTCTTTTAATATTTTTGCTCTTTTTTAAGTTTTCAATATCAGAAAACTGGTGGGGTTTGGTTTGTAACACACGGTTAAGGGTGATGAGTATCTTTGGTTGTAAGACTATGGAGAATGTTTGTAAAATAGTAGTGTGTTTAGAAGAATAGACCAGAGATGAATGCAAGTCCCTTCTCCGAATCATATTGTGGGTTGAAATTCAATTCCATCAAAAAACTGCTTTCAAAAGCAATTATGGTATGGCA

mRNA sequence

ATGGCTGTTCTTCTTCGAGCATTCTTTCTCTTCTCTCTCCTCATTCCATTTTCTTCGTCTTCTTCGATTGATGCTTCAAAGAAAACTTTCATTGTTCAGGTTCACAAAGATTCCAAGCCTTCTATTTTCCCAACCCACAAGAACTGGTATGAGTCCTCTTTAGCATCCATCTCCTCTGTCAATGATGTAGGAGCCATCATTCATACATATGAAACTCTTTTCCATGGCTTCTCTGCTAAGCTCTCACCTCTTGAAGTTGAGAAGCTTCAAACTCTCCCCCACGTCGCTTCCATCATTCCTGAACAGGTTCGTCATCCTCACACAACGCGTTCCCCTGAGTTTCTTGGCCTTAAGACATCTGATAGTGCTGGGTTGCTTAAAGAGTCCGACTTTGGATCTGACCTTGTAATTGGTGTTATTGATACTGGTATTTGGCCGGAGAGGCAGAGTTTTAACGACCGCGATCTGGGTCCTGTTCCTTCCAAATGGAAAGGTCAGTGTTTGGTGGCTAAAGACTTTCCAGCTACCTCTTGCAACCGTAAACTCATTGGGGCTCGATTTTTCTGTAGTGGGTACGAGGCTACCAATGGAAAGATGAATGAAACCACCGAGTATCGCTCGCCGCGAGATTCGGATGGCCATGGTACACATACAGCGTCCATTGCCGCAGGTCGTTATGTGTTTCCAGCTTCAACTTTGGGGTATGCTCGTGGTAAAGCTGCTGGAATGGCTCCTAAGGCCCGTCTTGCTGCTTATAAAGTGTGCTGGAACGCCGGCTGTTACGACTCCGACATTCTTGCTGCTTTTGATGCTGCCGTTTCCGACGGTGTTGATGTTGTATCACTCAGCGTCGGTGGTGTGGTTGTCCCGTACTATCTCGATGCCATCGCTATTGGGGCTTATCGAGCTGTGGCTGCCGGTGTTTTTGTCTCCGCATCGGCCGGTAATGGTGGTCCAGGTGGGCTCACTGTTACTAATGTAGCACCATGGGTTACAACAGTAGGAGCTGGAACCATGGATAGAGATTTTCCAGCTGATGTTAAGCTCGGAAATGGGAGGGTTGTACTTGGTACTAGTGTCTATGGCGGACCGGCTTTGATTCCGGGTCGTTTGTATCCTCTTATTTATGCAGGAACTGAAGGCGGTGATGGATATTCTTCGTCTCTATGCTTAGAAGGTTCGTTGAACCCCAATTTAGTGAAAGGGAAGATTGTACTTTGTGACAGGGGAATCAATTCAAGAGCTGCTAAAGGTGAGGTTGTGAAAAAGGCTGGAGGATTGGGAATGATTTTAGCAAATGGGGTCTTTGACGGTGAGGGTTTGGTAGCTGACTGCCACGTACTACCTGCAACCGCCGTCGGTGCTTCCGGCGGCGACGAGATTCGCAAATATATTGCAGAAGCAGCGAAGTCTCATTTACAGCCAACCGCTACAATTTTGTTCAAGGGAACTCGGCTTGGAGTTAGGCCAGCACCGGTTGTTGCTTCATTTTCAGCTCGAGGTCCAAATCCAGAGTCACCTGAAATTGTAAAGCCGGATGTGATTGCGCCTGGGTTGAACATTCTTGCCGCTTGGCCTGACAAAATTGGACCTTCTGGAATTCCCACAGATAAGCGTACCACTGAGTTCAACATACTTTCGGGCACGTCCATGGCCTGTCCTCATGTCTCTGGCTTAGCTGCCCTGCTGAAGGCTGCACACCCAGGATGGAGTCCAGCAGCTATAAAATCAGCCCTGATGACCACAGCTTATACTTTGGACAACCGGGGTGAGACGATGTTAGATGAATCTTCAGGCAATACATCCACGGTTTTGGACTTTGGAGCTGGCCATGTTCACCCACAAAAGGCAATGGACCCTGGTTTAATCTATGACCTGAATACATATGATTACGTTGATTTCTTGTGCAACTCCAATTACACTACTAAGAATATCCAAGTTATTACTGGGAAGATAGCAGATTGTAGTGGGGCGAAAAGGGCTGGACATACTGGGAATTTGAATTACCCTTCATTGGCCGTGGTGTTTCAACAATATGGTAAGCATAAGATGTCTACACATTTCATAAGAACTGTGACTAACGTTGGAGACGCCAATTCTATCTATAAGGTAACAATAAAGCCACCAAGTGGAATTTCAGTGACAGTTGAACCAGAAAAGCTGGCGTTTAGAAGGGTTGGGCAAAAATTGAGCTTCTTGGTTAGGGTCCAAGCAATGGCTGTTAGACTTTCCCCTGGAAGCTCTAGCATGAAGAGTGGTTCTATAATTTGGACTGATGGAAAGCACGAAGTCACTAGTCCTTTGGTTGTTACCATGCAGCAACCTCTGCAGTAG

Coding sequence (CDS)

ATGGCTGTTCTTCTTCGAGCATTCTTTCTCTTCTCTCTCCTCATTCCATTTTCTTCGTCTTCTTCGATTGATGCTTCAAAGAAAACTTTCATTGTTCAGGTTCACAAAGATTCCAAGCCTTCTATTTTCCCAACCCACAAGAACTGGTATGAGTCCTCTTTAGCATCCATCTCCTCTGTCAATGATGTAGGAGCCATCATTCATACATATGAAACTCTTTTCCATGGCTTCTCTGCTAAGCTCTCACCTCTTGAAGTTGAGAAGCTTCAAACTCTCCCCCACGTCGCTTCCATCATTCCTGAACAGGTTCGTCATCCTCACACAACGCGTTCCCCTGAGTTTCTTGGCCTTAAGACATCTGATAGTGCTGGGTTGCTTAAAGAGTCCGACTTTGGATCTGACCTTGTAATTGGTGTTATTGATACTGGTATTTGGCCGGAGAGGCAGAGTTTTAACGACCGCGATCTGGGTCCTGTTCCTTCCAAATGGAAAGGTCAGTGTTTGGTGGCTAAAGACTTTCCAGCTACCTCTTGCAACCGTAAACTCATTGGGGCTCGATTTTTCTGTAGTGGGTACGAGGCTACCAATGGAAAGATGAATGAAACCACCGAGTATCGCTCGCCGCGAGATTCGGATGGCCATGGTACACATACAGCGTCCATTGCCGCAGGTCGTTATGTGTTTCCAGCTTCAACTTTGGGGTATGCTCGTGGTAAAGCTGCTGGAATGGCTCCTAAGGCCCGTCTTGCTGCTTATAAAGTGTGCTGGAACGCCGGCTGTTACGACTCCGACATTCTTGCTGCTTTTGATGCTGCCGTTTCCGACGGTGTTGATGTTGTATCACTCAGCGTCGGTGGTGTGGTTGTCCCGTACTATCTCGATGCCATCGCTATTGGGGCTTATCGAGCTGTGGCTGCCGGTGTTTTTGTCTCCGCATCGGCCGGTAATGGTGGTCCAGGTGGGCTCACTGTTACTAATGTAGCACCATGGGTTACAACAGTAGGAGCTGGAACCATGGATAGAGATTTTCCAGCTGATGTTAAGCTCGGAAATGGGAGGGTTGTACTTGGTACTAGTGTCTATGGCGGACCGGCTTTGATTCCGGGTCGTTTGTATCCTCTTATTTATGCAGGAACTGAAGGCGGTGATGGATATTCTTCGTCTCTATGCTTAGAAGGTTCGTTGAACCCCAATTTAGTGAAAGGGAAGATTGTACTTTGTGACAGGGGAATCAATTCAAGAGCTGCTAAAGGTGAGGTTGTGAAAAAGGCTGGAGGATTGGGAATGATTTTAGCAAATGGGGTCTTTGACGGTGAGGGTTTGGTAGCTGACTGCCACGTACTACCTGCAACCGCCGTCGGTGCTTCCGGCGGCGACGAGATTCGCAAATATATTGCAGAAGCAGCGAAGTCTCATTTACAGCCAACCGCTACAATTTTGTTCAAGGGAACTCGGCTTGGAGTTAGGCCAGCACCGGTTGTTGCTTCATTTTCAGCTCGAGGTCCAAATCCAGAGTCACCTGAAATTGTAAAGCCGGATGTGATTGCGCCTGGGTTGAACATTCTTGCCGCTTGGCCTGACAAAATTGGACCTTCTGGAATTCCCACAGATAAGCGTACCACTGAGTTCAACATACTTTCGGGCACGTCCATGGCCTGTCCTCATGTCTCTGGCTTAGCTGCCCTGCTGAAGGCTGCACACCCAGGATGGAGTCCAGCAGCTATAAAATCAGCCCTGATGACCACAGCTTATACTTTGGACAACCGGGGTGAGACGATGTTAGATGAATCTTCAGGCAATACATCCACGGTTTTGGACTTTGGAGCTGGCCATGTTCACCCACAAAAGGCAATGGACCCTGGTTTAATCTATGACCTGAATACATATGATTACGTTGATTTCTTGTGCAACTCCAATTACACTACTAAGAATATCCAAGTTATTACTGGGAAGATAGCAGATTGTAGTGGGGCGAAAAGGGCTGGACATACTGGGAATTTGAATTACCCTTCATTGGCCGTGGTGTTTCAACAATATGGTAAGCATAAGATGTCTACACATTTCATAAGAACTGTGACTAACGTTGGAGACGCCAATTCTATCTATAAGGTAACAATAAAGCCACCAAGTGGAATTTCAGTGACAGTTGAACCAGAAAAGCTGGCGTTTAGAAGGGTTGGGCAAAAATTGAGCTTCTTGGTTAGGGTCCAAGCAATGGCTGTTAGACTTTCCCCTGGAAGCTCTAGCATGAAGAGTGGTTCTATAATTTGGACTGATGGAAAGCACGAAGTCACTAGTCCTTTGGTTGTTACCATGCAGCAACCTCTGCAGTAG
BLAST of CSPI07G22140 vs. Swiss-Prot
Match: SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 591/779 (75.87%), Postives = 692/779 (88.83%), Query Frame = 1

Query: 1   MAVLLRAFFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSV 60
           MA     FFL +L  P SS+SS  ++  T+IV V  ++KPSIFPTH +WY SSLAS++S 
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASS--SNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTS- 60

Query: 61  NDVGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTS 120
               +IIHTY+T+FHGFSA+L+  +  +L   PHV S+IPEQVRH HTTRSPEFLGL+++
Sbjct: 61  -SPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRST 120

Query: 121 DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNR 180
           D AGLL+ESDFGSDLVIGVIDTG+WPER SF+DR LGPVP KWKGQC+ ++DFP ++CNR
Sbjct: 121 DKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNR 180

Query: 181 KLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKA 240
           KL+GARFFC GYEATNGKMNETTE+RSPRDSDGHGTHTASI+AGRYVFPASTLGYA G A
Sbjct: 181 KLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVA 240

Query: 241 AGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA 300
           AGMAPKARLAAYKVCWN+GCYDSDILAAFD AV+DGVDV+SLSVGGVVVPYYLDAIAIGA
Sbjct: 241 AGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGA 300

Query: 301 YRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSV 360
           + A+  G+FVSASAGNGGPG LTVTNVAPW+TTVGAGT+DRDFPA+VKLGNG+++ G SV
Sbjct: 301 FGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSV 360

Query: 361 YGGPALIPGRLYPLIYAGT-EGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGE 420
           YGGP L PGR+YPL+Y G+  GGDGYSSSLCLEGSL+PNLVKGKIVLCDRGINSRA KGE
Sbjct: 361 YGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGE 420

Query: 421 VVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSH--LQPTA 480
           +V+K GGLGMI+ANGVFDGEGLVADCHVLPAT+VGASGGDEIR+YI+E++KS     PTA
Sbjct: 421 IVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTA 480

Query: 481 TILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTD 540
           TI+FKGTRLG+RPAPVVASFSARGPNPE+PEI+KPDVIAPGLNILAAWPD+IGPSG+ +D
Sbjct: 481 TIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSD 540

Query: 541 KRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDES 600
            R TEFNILSGTSMACPHVSGLAALLKAAHP WSPAAI+SAL+TTAYT+DN GE M+DES
Sbjct: 541 NRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDES 600

Query: 601 SGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSG 660
           +GNTS+V+D+G+GHVHP KAMDPGL+YD+ +YDY++FLCNSNYT  NI  IT + ADC G
Sbjct: 601 TGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDG 660

Query: 661 AKRAGHTGNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVE 720
           A+RAGH GNLNYPS +VVFQQYG+ KMSTHFIRTVTNVGD++S+Y++ I+PP G +VTVE
Sbjct: 661 ARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVE 720

Query: 721 PEKLAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPL 777
           PEKL+FRRVGQKLSF+VRV+   V+LSPG++++++G I+W+DGK  VTSPLVVT+QQPL
Sbjct: 721 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

BLAST of CSPI07G22140 vs. Swiss-Prot
Match: SBT16_ARATH (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 806.6 bits (2082), Expect = 2.4e-232
Identity = 418/773 (54.08%), Postives = 547/773 (70.76%), Query Frame = 1

Query: 10  LFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSVNDVGAIIHT 69
           L  L  PF S ++  A+K TFI ++   S PSIFPTH +WY +  A  S       I+H 
Sbjct: 9   LLFLSFPFISFAASQAAK-TFIFRIDGGSMPSIFPTHYHWYSTEFAEES------RIVHV 68

Query: 70  YETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTSDSAGLLKES 129
           Y T+FHGFSA ++P E + L+  P V ++  ++ R  HTTRSP+FLGL+  +  GL  ES
Sbjct: 69  YHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ--NQKGLWSES 128

Query: 130 DFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNRKLIGARFFC 189
           D+GSD++IGV DTGIWPER+SF+D +LGP+P +W+G C     F   +CNRK+IGARFF 
Sbjct: 129 DYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFA 188

Query: 190 SGYE-ATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKAR 249
            G + A  G +N+T E+ SPRD+DGHGTHT+S AAGR+ F AS  GYA G A G+APKAR
Sbjct: 189 KGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKAR 248

Query: 250 LAAYKVCW-NAGCYDSDILAAFDAAVSDGVDVVSLSVG---GVVVPYYLDAIAIGAYRAV 309
           +AAYKVCW ++GC DSDILAAFDAAV DGVDV+S+S+G   G+  PYYLD IAIG+Y A 
Sbjct: 249 IAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAA 308

Query: 310 AAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGP 369
           + G+FVS+SAGN GP G++VTN+APWVTTVGA T+DR+FPAD  LG+G  + G S+Y G 
Sbjct: 309 SKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGV 368

Query: 370 ALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKA 429
            L  GR++P++Y G  G    S+SLC+E +L+P  V+GKIV+CDRG + R AKG VVKKA
Sbjct: 369 PL-NGRMFPVVYPGKSGMS--SASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKA 428

Query: 430 GGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGT 489
           GG+GMILANG  +GEGLV D H++PA AVG++ GD I+ Y    A SH  P A+I F+GT
Sbjct: 429 GGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAY----ASSHPNPIASIDFRGT 488

Query: 490 RLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFN 549
            +G++PAPV+ASFS RGPN  SPEI+KPD+IAPG+NILAAW D +GP+G+P+D R TEFN
Sbjct: 489 IVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFN 548

Query: 550 ILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTV 609
           ILSGTSMACPHVSG AALLK+AHP WSPA I+SA+MTT   +DN   +++DES+G ++T 
Sbjct: 549 ILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATP 608

Query: 610 LDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHT 669
            D+G+GH++  +AM+PGL+YD+   DY+ FLC+  Y  K IQVIT     C   ++    
Sbjct: 609 YDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPS-P 668

Query: 670 GNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFR 729
           GNLNYPS+  VF    +  +S   IRT TNVG A ++Y+  I+ P G++VTV+P +L F 
Sbjct: 669 GNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFT 728

Query: 730 RVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTD-GKHEVTSPLVVTMQQPL 777
              ++ S+ V V      +  G +    GS+ W D GKH V SP+VVT    L
Sbjct: 729 SAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of CSPI07G22140 vs. Swiss-Prot
Match: SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 8.5e-214
Identity = 399/765 (52.16%), Postives = 513/765 (67.06%), Query Frame = 1

Query: 7   AFFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSVNDVGAI 66
           AFFL  L + F   SS  + + T+IV + K   PS F  H NWY+SSL SIS   D   +
Sbjct: 10  AFFLL-LCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSIS---DSAEL 69

Query: 67  IHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTSDSAGLL 126
           ++TYE   HGFS +L+  E + L T P V S++PE     HTTR+P FLGL    +A L 
Sbjct: 70  LYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD-EHTADLF 129

Query: 127 KESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNRKLIGAR 186
            E+   SD+V+GV+DTG+WPE +S++D   GP+PS WKG C    +F A+ CNRKLIGAR
Sbjct: 130 PEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGAR 189

Query: 187 FFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPK 246
           FF  GYE+T G ++E+ E RSPRD DGHGTHT+S AAG  V  AS LGYA G A GMAP+
Sbjct: 190 FFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPR 249

Query: 247 ARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAA 306
           AR+A YKVCW  GC+ SDILAA D A++D V+V+S+S+GG +  YY D +AIGA+ A+  
Sbjct: 250 ARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMER 309

Query: 307 GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPAL 366
           G+ VS SAGN GP   +++NVAPW+TTVGAGT+DRDFPA   LGNG+   G S++ G AL
Sbjct: 310 GILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEAL 369

Query: 367 IPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGG 426
            P +L P IYAG    +  + +LC+ G+L P  VKGKIV+CDRGIN+R  KG+VVK AGG
Sbjct: 370 -PDKLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGG 429

Query: 427 LGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRL 486
           +GMILAN   +GE LVAD H+LPAT VG   GD IR Y+     +   PTA+I   GT +
Sbjct: 430 VGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVT----TDPNPTASISILGTVV 489

Query: 487 GVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNIL 546
           GV+P+PVVA+FS+RGPN  +P I+KPD+IAPG+NILAAW    GP+G+ +D R  EFNI+
Sbjct: 490 GVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNII 549

Query: 547 SGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLD 606
           SGTSM+CPHVSGLAALLK+ HP WSPAAI+SALMTTAY     G+ +LD ++G  ST  D
Sbjct: 550 SGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFD 609

Query: 607 FGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGN 666
            GAGHV P  A +PGLIYDL T DY+ FLC  NYT+  I+ ++ +   C  +K +    +
Sbjct: 610 HGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSK-SYSVAD 669

Query: 667 LNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRV 726
           LNYPS AV     G +K    + RTVT+VG A +         +G+ ++VEP  L F+  
Sbjct: 670 LNYPSFAVNVDGVGAYK----YTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEA 729

Query: 727 GQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVT 772
            +K S+ V      V  S  S S   GSI W+DGKH V SP+ ++
Sbjct: 730 NEKKSYTV---TFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of CSPI07G22140 vs. Swiss-Prot
Match: SBT14_ARATH (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 3.2e-205
Identity = 393/777 (50.58%), Postives = 507/777 (65.25%), Query Frame = 1

Query: 8   FFLFSLLIPF-SSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSVNDVGAI 67
           FF+F LL+ F S SSS     +++IV V +  KPS+F +H NW+ S L S+ S      +
Sbjct: 10  FFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATL 69

Query: 68  IHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTSDSAGLL 127
           +++Y    HGFSA+LSP++   L+  P V S+IP+Q R  HTT +P FLG   S ++GL 
Sbjct: 70  LYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF--SQNSGLW 129

Query: 128 KESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNRKLIGAR 187
             S++G D+++GV+DTGIWPE  SF+D  LGP+PS WKG+C +  DFPA+SCNRKLIGAR
Sbjct: 130 SNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGAR 189

Query: 188 FFCSGY--EATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMA 247
            F  GY  +    K +   E RSPRD++GHGTHTAS AAG  V  AS   YARG A GMA
Sbjct: 190 AFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMA 249

Query: 248 PKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVG--GVVVPYYLDAIAIGAYR 307
            KAR+AAYK+CW  GCYDSDILAA D AV+DGV V+SLSVG  G    Y+ D+IAIGA+ 
Sbjct: 250 SKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFG 309

Query: 308 AVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYG 367
           A   G+ VS SAGN GP   T TN+APW+ TVGA T+DR+F A+   G+G+V  GTS+Y 
Sbjct: 310 ATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYA 369

Query: 368 GPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVK 427
           G +L P     L+Y+G  G     S LC  G LN +LV+GKIVLCDRG N+R  KG  VK
Sbjct: 370 GESL-PDSQLSLVYSGDCG-----SRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVK 429

Query: 428 KAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFK 487
            AGG GMILAN    GE L AD H++PAT VGA  GD+IR YI    K+   PTA I F 
Sbjct: 430 LAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYI----KTSDSPTAKISFL 489

Query: 488 GTRLGVRP-APVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTT 547
           GT +G  P +P VA+FS+RGPN  +P I+KPDVIAPG+NILA W   +GP+ +  D R  
Sbjct: 490 GTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRV 549

Query: 548 EFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNT 607
           +FNI+SGTSM+CPHVSGLAALL+ AHP WSPAAIKSAL+TTAY ++N GE + D ++G +
Sbjct: 550 QFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKS 609

Query: 608 STVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVI--TGKIADCSGAK 667
           S     GAGHV P KA++PGL+YD+   +YV FLC   Y    I V      + D     
Sbjct: 610 SNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETS 669

Query: 668 RAGHTGNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVG-DANSIYKVTIKPPSGISVTVEP 727
           +    G+LNYPS +VVF   G+      + R V NVG + +++Y+V +K P+ + + V P
Sbjct: 670 KLRTAGDLNYPSFSVVFASTGE---VVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSP 729

Query: 728 EKLAFRRVGQKLSFLVRVQAMAVRLSPGS-SSMKSGSIIWTDGKHEVTSPLVVTMQQ 775
            KLAF +    L + V  +++ +    GS    + GSI WTDG+H V SP+ V   Q
Sbjct: 730 SKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQ 771

BLAST of CSPI07G22140 vs. Swiss-Prot
Match: SBT18_ARATH (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 9.4e-205
Identity = 384/746 (51.47%), Postives = 504/746 (67.56%), Query Frame = 1

Query: 26  SKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSVNDVGAIIHTYETLFHGFSAKLSPLE 85
           +KKT+I++V+   KP  F TH +WY S L S SS      +++TY T FHGFSA L   E
Sbjct: 26  AKKTYIIRVNHSDKPESFLTHHDWYTSQLNSESS------LLYTYTTSFHGFSAYLDSTE 85

Query: 86  VEKLQTLPH-VASIIPEQVRHPHTTRSPEFLGLKTSDSAGLLKESDFGSDLVIGVIDTGI 145
            + L +  + +  I  + +   HTTR+PEFLGL +      L  S  G  ++IGV+DTG+
Sbjct: 86  ADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNG--VIIGVLDTGV 145

Query: 146 WPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNRKLIGARFFCSGYE-ATNGKMNETT 205
           WPE +SF+D D+  +PSKWKG+C    DF +  CN+KLIGAR F  G++ A+ G  +   
Sbjct: 146 WPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKR 205

Query: 206 EYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARLAAYKVCWNAGCYDS 265
           E  SPRD DGHGTHT++ AAG  V  AS LGYA G A GMA +AR+A YKVCW+ GC+ S
Sbjct: 206 ESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGS 265

Query: 266 DILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVFVSASAGNGGPGGLT 325
           DILAA D A+ DGVDV+SLS+GG   PYY D IAIGA+ A+  GVFVS SAGN GP   +
Sbjct: 266 DILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRAS 325

Query: 326 VTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPGRLYPLIYAGTEGGD 385
           V NVAPWV TVGAGT+DRDFPA   LGNG+ + G S+Y G  +    L  L+Y     G+
Sbjct: 326 VANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPL-ELVY---NKGN 385

Query: 386 GYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVA 445
             SS+LCL GSL+ ++V+GKIV+CDRG+N+R  KG VV+ AGGLGMI+AN    GE LVA
Sbjct: 386 SSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVA 445

Query: 446 DCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVRPAPVVASFSARGPN 505
           D H+LPA AVG   GD +R+Y+    KS  +PTA ++FKGT L V+P+PVVA+FS+RGPN
Sbjct: 446 DSHLLPAIAVGKKTGDLLREYV----KSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPN 505

Query: 506 PESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGTSMACPHVSGLAALL 565
             +PEI+KPDVI PG+NILA W D IGP+G+  D R T+FNI+SGTSM+CPH+SGLA LL
Sbjct: 506 TVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLL 565

Query: 566 KAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLI 625
           KAAHP WSP+AIKSALMTTAY LDN    + D +  + S     G+GHV PQKA+ PGL+
Sbjct: 566 KAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLV 625

Query: 626 YDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNYPSLAVVFQQYGKHK 685
           YD++T +Y+ FLC+ +YT  +I  I  K    + +K+    G LNYPS +V+F      K
Sbjct: 626 YDISTEEYIRFLCSLDYTVDHIVAIV-KRPSVNCSKKFSDPGQLNYPSFSVLF----GGK 685

Query: 686 MSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQKLSFLVR-VQAMAVR 745
               + R VTNVG A+S+YKVT+     + ++V+P KL+F+ VG+K  + V  V    V 
Sbjct: 686 RVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVS 745

Query: 746 LSPGSSSMKSGSIIWTDGKHEVTSPL 769
           +   ++  + GSI W++ +HEV SP+
Sbjct: 746 M---TNKAEFGSITWSNPQHEVRSPV 747

BLAST of CSPI07G22140 vs. TrEMBL
Match: A0A0A0KAP0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G448090 PE=4 SV=1)

HSP 1 Score: 1539.2 bits (3984), Expect = 0.0e+00
Identity = 776/777 (99.87%), Postives = 777/777 (100.00%), Query Frame = 1

Query: 1   MAVLLRAFFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSV 60
           MAVLLRAFFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSV
Sbjct: 1   MAVLLRAFFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSV 60

Query: 61  NDVGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTS 120
           NDVGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTS
Sbjct: 61  NDVGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTS 120

Query: 121 DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNR 180
           DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNR
Sbjct: 121 DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNR 180

Query: 181 KLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKA 240
           KLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKA
Sbjct: 181 KLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKA 240

Query: 241 AGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA 300
           AGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA
Sbjct: 241 AGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA 300

Query: 301 YRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSV 360
           YRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSV
Sbjct: 301 YRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSV 360

Query: 361 YGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEV 420
           YGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEV
Sbjct: 361 YGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEV 420

Query: 421 VKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATIL 480
           VKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATIL
Sbjct: 421 VKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATIL 480

Query: 481 FKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRT 540
           FKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRT
Sbjct: 481 FKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRT 540

Query: 541 TEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGN 600
           TEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGN
Sbjct: 541 TEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGN 600

Query: 601 TSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKR 660
           TSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKR
Sbjct: 601 TSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKR 660

Query: 661 AGHTGNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEK 720
           AGH+GNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEK
Sbjct: 661 AGHSGNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEK 720

Query: 721 LAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 778
           LAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ
Sbjct: 721 LAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 777

BLAST of CSPI07G22140 vs. TrEMBL
Match: W9R4Z9_9ROSA (Subtilisin-like protease OS=Morus notabilis GN=L484_002479 PE=4 SV=1)

HSP 1 Score: 1309.7 bits (3388), Expect = 0.0e+00
Identity = 651/785 (82.93%), Postives = 708/785 (90.19%), Query Frame = 1

Query: 4   LLRAFFLFSLLIPF-----SSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASIS 63
           L  +FFL+  L  F     SS S  D  KKTFIVQV + +KPSIF THKNWYESSL+SIS
Sbjct: 3   LSSSFFLYLFLTIFLSLASSSCSDNDDHKKTFIVQVQRQAKPSIFSTHKNWYESSLSSIS 62

Query: 64  S-------VNDVGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRS 123
           S         D   IIHTY+T+F GFSAKL+ LE +KL+TLPHV ++IPEQVR  HTTRS
Sbjct: 63  SSPDNKTTTLDASTIIHTYDTVFDGFSAKLTSLEAQKLRTLPHVLAVIPEQVRRLHTTRS 122

Query: 124 PEFLGLKTSDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAK 183
           PEFLGLK +DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDL PVPSKWKGQC+  K
Sbjct: 123 PEFLGLKKTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLDPVPSKWKGQCVAGK 182

Query: 184 DFPATSCNRKLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPAS 243
           DFPAT CNRKLIGARFFC GYE+TNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPAS
Sbjct: 183 DFPATFCNRKLIGARFFCEGYESTNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPAS 242

Query: 244 TLGYARGKAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPY 303
           TLGYARG AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAV+DGVDV+SLSVGGVVVPY
Sbjct: 243 TLGYARGVAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVADGVDVISLSVGGVVVPY 302

Query: 304 YLDAIAIGAYRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN 363
           YLD+IAIGA+ A   GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN
Sbjct: 303 YLDSIAIGAFGAADNGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN 362

Query: 364 GRVVLGTSVYGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGI 423
           GR + G SVYGGP L PGR+Y LIYAG EG DGYSSSLCLEGSLNP+ VKGKIVLCDRGI
Sbjct: 363 GRTIPGVSVYGGPDLSPGRMYSLIYAGNEGSDGYSSSLCLEGSLNPSSVKGKIVLCDRGI 422

Query: 424 NSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKS 483
           NSRA KGEVVKKAGG+GMILANGVFDGEGLVADCHVLPAT+VGAS GDEIRKYI  A+KS
Sbjct: 423 NSRATKGEVVKKAGGVGMILANGVFDGEGLVADCHVLPATSVGASSGDEIRKYITSASKS 482

Query: 484 HLQPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGP 543
              PTATILFKGT+LG+RPAPVVASFSARGPNPESPEI+KPDVIAPGLNILAAWPD++GP
Sbjct: 483 RSPPTATILFKGTKLGIRPAPVVASFSARGPNPESPEILKPDVIAPGLNILAAWPDRVGP 542

Query: 544 SGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGE 603
           SGIP+DKR TEFNILSGTSMACPHVSGLAALLKAAHP WSPAAI+SALMTTAYT+DNRGE
Sbjct: 543 SGIPSDKRRTEFNILSGTSMACPHVSGLAALLKAAHPEWSPAAIRSALMTTAYTVDNRGE 602

Query: 604 TMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGK 663
           T+LDES+GN+STV+DFGAGHVHP+KA+DPGL+YD+ +YDYVDFLCNSNYTTKNIQVIT K
Sbjct: 603 TVLDESTGNSSTVMDFGAGHVHPEKAIDPGLVYDITSYDYVDFLCNSNYTTKNIQVITRK 662

Query: 664 IADCSGAKRAGHTGNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSG 723
            ADCSGAK+AGH GNLNYPSL+ +FQQYG+HKMSTHFIRTVTNVGD NS+Y+VTI PPSG
Sbjct: 663 SADCSGAKKAGHAGNLNYPSLSALFQQYGRHKMSTHFIRTVTNVGDPNSVYRVTISPPSG 722

Query: 724 ISVTVEPEKLAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVT 777
             VTVEPEKLAFRRVGQ+L+FLVRV+A AV+LSPG+S +KSGSI+W+DGKH+VTSPLVVT
Sbjct: 723 TVVTVEPEKLAFRRVGQRLNFLVRVEATAVKLSPGNSYVKSGSIVWSDGKHKVTSPLVVT 782

BLAST of CSPI07G22140 vs. TrEMBL
Match: A0A061GDY7_THECC (Subtilase family protein isoform 1 OS=Theobroma cacao GN=TCM_029233 PE=4 SV=1)

HSP 1 Score: 1286.2 bits (3327), Expect = 0.0e+00
Identity = 630/769 (81.92%), Postives = 699/769 (90.90%), Query Frame = 1

Query: 8   FFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSVNDVGAII 67
           FF  SLL   SS+S +D  +KTFIV+V  D KPSIF THK+WYESSL+S+ S +    ++
Sbjct: 6   FFFLSLLSLASSASRVD--QKTFIVRVQHDVKPSIFTTHKHWYESSLSSVLSPSTPTQVL 65

Query: 68  HTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTSDSAGLLK 127
           H Y+ +FHGFSAKLSP E  KLQTLPH+ ++IPEQVRH  TTRSP FLGLKT+DSAGLLK
Sbjct: 66  HVYDNVFHGFSAKLSPTEALKLQTLPHIIAVIPEQVRHVQTTRSPLFLGLKTTDSAGLLK 125

Query: 128 ESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNRKLIGARF 187
           ESDFGSDLVIGVIDTGIWPERQSFNDRDLGP+PSKWKGQC+  KDF ++SCN+KLIGA+F
Sbjct: 126 ESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTTKDFGSSSCNKKLIGAKF 185

Query: 188 FCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKA 247
           FC+GYEATNGKMNET+E+RSPRDSDGHGTHTASIAAGRYVFPASTLGYA+G AAGMAPKA
Sbjct: 186 FCNGYEATNGKMNETSEFRSPRDSDGHGTHTASIAAGRYVFPASTLGYAKGVAAGMAPKA 245

Query: 248 RLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAG 307
           RLAAYKVCWNAGCYDSDILAAFDAAV+DGVDV+SLSVGGVVVPYYLDAIAIGA+ A   G
Sbjct: 246 RLAAYKVCWNAGCYDSDILAAFDAAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAADKG 305

Query: 308 VFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALI 367
           +FVSASAGNGGPGGL+VTNVAPWV TVGAGT+DRDFPADVKLGNG+VV G SVY GP L 
Sbjct: 306 IFVSASAGNGGPGGLSVTNVAPWVATVGAGTIDRDFPADVKLGNGKVVPGVSVYNGPGLS 365

Query: 368 PGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGL 427
           PGR+YPL+YAGT GGDGYSSSLC+EGSL+P+ VKGK+VLCDRGINSRAAKGEVVKKAGG+
Sbjct: 366 PGRMYPLVYAGTGGGDGYSSSLCMEGSLDPDFVKGKLVLCDRGINSRAAKGEVVKKAGGI 425

Query: 428 GMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLG 487
           GMILANGVFDGEGLVADCHVLPATAVGA+ GDEIR+YI  A+KS    TATI+FKGTRLG
Sbjct: 426 GMILANGVFDGEGLVADCHVLPATAVGAANGDEIRRYIDSASKSKSPATATIVFKGTRLG 485

Query: 488 VRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILS 547
           VRPAPVVASFSARGPNPE+PEI+KPDVIAPGLNILAAWPDK+GPSG+ +DKR TEFNILS
Sbjct: 486 VRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDKVGPSGVASDKRRTEFNILS 545

Query: 548 GTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDF 607
           GTSMACPHVSGLAALLKAAH  WSPAAIKSALMTTAYT+DNRGETMLDESSGNTSTVLDF
Sbjct: 546 GTSMACPHVSGLAALLKAAHSEWSPAAIKSALMTTAYTVDNRGETMLDESSGNTSTVLDF 605

Query: 608 GAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNL 667
           G+GHVHP KAMDPGL+YD+ + DYVDFLCNSNYT  NIQVIT + ADCSGAKRAGH GNL
Sbjct: 606 GSGHVHPTKAMDPGLVYDITSMDYVDFLCNSNYTINNIQVITRRNADCSGAKRAGHIGNL 665

Query: 668 NYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVG 727
           NYPS + VFQQYGKHKMSTHF+R VTNVGD NS+YKVT++PPSG  VTVEPE+L FRRVG
Sbjct: 666 NYPSFSAVFQQYGKHKMSTHFLRQVTNVGDPNSVYKVTVRPPSGTLVTVEPEQLVFRRVG 725

Query: 728 QKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPL 777
           QKL+FLVRVQA+AV+LSPGS++MKSGSI+W+DGKH VTSPL+VTMQQPL
Sbjct: 726 QKLNFLVRVQAVAVKLSPGSTNMKSGSIVWSDGKHNVTSPLIVTMQQPL 772

BLAST of CSPI07G22140 vs. TrEMBL
Match: F6HXP0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g00450 PE=4 SV=1)

HSP 1 Score: 1283.9 bits (3321), Expect = 0.0e+00
Identity = 632/782 (80.82%), Postives = 706/782 (90.28%), Query Frame = 1

Query: 3   VLLRAFFLFSLL--IPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSV 62
           + L  FF FS L  +PF S+S+  A  +T+IV V  D+KPS+FPTHK+WY+SSL S+SS 
Sbjct: 7   LFLSLFFFFSFLPFLPFYSASA-SALHRTYIVFVQHDAKPSVFPTHKHWYDSSLRSLSST 66

Query: 63  ------NDVGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEF 122
                 ++   I+HTYET+FHGFSAKLSPLE ++LQ +  +  +IPEQVR   TTRSP+F
Sbjct: 67  IQTTSHSETSRILHTYETVFHGFSAKLSPLEADQLQKVSGIVGVIPEQVRELQTTRSPQF 126

Query: 123 LGLKTSDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFP 182
           LGLKT+DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDR+LGPVP+KWKG+C+  KDFP
Sbjct: 127 LGLKTTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRNLGPVPAKWKGECVGGKDFP 186

Query: 183 ATSCNRKLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLG 242
           ATSCNRKLIGARFFC GYEATNGKMNET E RSPRDSDGHGTHTASIAAGRYVFPASTLG
Sbjct: 187 ATSCNRKLIGARFFCGGYEATNGKMNETLESRSPRDSDGHGTHTASIAAGRYVFPASTLG 246

Query: 243 YARGKAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLD 302
           YARG AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAV+DG DVVSLSVGGVVVPYYLD
Sbjct: 247 YARGVAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVADGADVVSLSVGGVVVPYYLD 306

Query: 303 AIAIGAYRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRV 362
           +IAIGA+ A   GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPA+VKLGNG++
Sbjct: 307 SIAIGAFGASDHGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPANVKLGNGKL 366

Query: 363 VLGTSVYGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSR 422
           + G SVYGGP L PGRLYPLIYAG+ GGDGYSSSLCLEGSL+P+ VKGKIVLCDRGINSR
Sbjct: 367 IPGVSVYGGPGLAPGRLYPLIYAGSVGGDGYSSSLCLEGSLDPSFVKGKIVLCDRGINSR 426

Query: 423 AAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQ 482
           A KGEVV+KAGG+GMILANGVFDGEGLVADCHVLPATA+GASGGDEIRKYI  A+KS   
Sbjct: 427 ATKGEVVRKAGGIGMILANGVFDGEGLVADCHVLPATAIGASGGDEIRKYITVASKSKSP 486

Query: 483 PTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGI 542
           PTATI+F+GTRLGVRPAPVVASFSARGPNPESPEI+KPDVIAPGLNILAAWPD++GPSGI
Sbjct: 487 PTATIIFRGTRLGVRPAPVVASFSARGPNPESPEILKPDVIAPGLNILAAWPDRVGPSGI 546

Query: 543 PTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETML 602
           P+DKR TEFNILSGTSMACPH+SGLAALLKAAHP WSPAAI+SALMTTAYT DNRGETML
Sbjct: 547 PSDKRRTEFNILSGTSMACPHISGLAALLKAAHPEWSPAAIRSALMTTAYTEDNRGETML 606

Query: 603 DESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIAD 662
           DE++GNTSTV+DFGAGHVHPQKAMDPGLIYDL + DY+DFLCNSNYT  NIQ+IT K+AD
Sbjct: 607 DEATGNTSTVMDFGAGHVHPQKAMDPGLIYDLTSNDYIDFLCNSNYTVTNIQMITRKMAD 666

Query: 663 CSGAKRAGHTGNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISV 722
           CS A++AGH GNLNYPS++ VFQQYGKHK STHFIRTVTNVGD NS+Y+VT+KPP+G  V
Sbjct: 667 CSKARKAGHVGNLNYPSMSAVFQQYGKHKFSTHFIRTVTNVGDPNSVYQVTVKPPTGTLV 726

Query: 723 TVEPEKLAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQ 777
           TV+PEKL FRR+GQKL+FLVRV+AMAV+LSPGS+S+KSGSI+W DGKH VTSP+VVT++Q
Sbjct: 727 TVQPEKLVFRRLGQKLNFLVRVEAMAVKLSPGSTSIKSGSIVWADGKHTVTSPIVVTLEQ 786

BLAST of CSPI07G22140 vs. TrEMBL
Match: A0A059ARI3_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I02156 PE=4 SV=1)

HSP 1 Score: 1280.0 bits (3311), Expect = 0.0e+00
Identity = 629/774 (81.27%), Postives = 704/774 (90.96%), Query Frame = 1

Query: 8   FFLF-SLLIPFSSSSSIDASK---KTFIVQVHKDSKPSIFPTHKNWYESSLASISS-VND 67
           FF+F ++L+P SSSSS  +S+   KTFIVQV  D+KPS+FPTH +WY+S+L+S+SS    
Sbjct: 124 FFIFLAILLPLSSSSSSSSSEEAAKTFIVQVQPDAKPSVFPTHAHWYDSTLSSLSSSAGP 183

Query: 68  VGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTSDS 127
              ++HTY T+FHGFSAKLSP +  KL +LPHV ++IPEQVR  HTTRSP+FLGLKT DS
Sbjct: 184 TARVLHTYSTVFHGFSAKLSPSQALKLWSLPHVLALIPEQVRRLHTTRSPQFLGLKTGDS 243

Query: 128 AGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNRKL 187
           AGLLKESDFGSDLVIGV+DTGIWPERQSFNDRDLGPVPSKWKG+C+  K FP+TSCNRKL
Sbjct: 244 AGLLKESDFGSDLVIGVVDTGIWPERQSFNDRDLGPVPSKWKGECVAGKAFPSTSCNRKL 303

Query: 188 IGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAG 247
           IGARFF +GYEA NGKMNETT++RSPRDSDGHGTHTASIAAGRYVFPASTLGYA+G AAG
Sbjct: 304 IGARFFYNGYEAANGKMNETTQFRSPRDSDGHGTHTASIAAGRYVFPASTLGYAKGMAAG 363

Query: 248 MAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYR 307
           MAPKARLAAYKVCW+AGCYDSDILAAFDAAV DGVDV+SLSVGGVVVPY+LDAIAIGA+ 
Sbjct: 364 MAPKARLAAYKVCWDAGCYDSDILAAFDAAVGDGVDVISLSVGGVVVPYHLDAIAIGAFG 423

Query: 308 AVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYG 367
           A  AGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGT+DRDFPADVKLGNG+++ G S+YG
Sbjct: 424 AEEAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTIDRDFPADVKLGNGKMIPGVSLYG 483

Query: 368 GPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVK 427
           GP L PGR+YPLIYAGTEGGDGYS+SLC+EGSL+ +LVK KIVLCDRGINSRAAKGEVVK
Sbjct: 484 GPGLTPGRMYPLIYAGTEGGDGYSASLCMEGSLDQSLVKDKIVLCDRGINSRAAKGEVVK 543

Query: 428 KAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFK 487
           KAGG+GMILANGVFDGEGLVADCHVLPATAVGA+ GDEIRKYI  A+KS    TAT++FK
Sbjct: 544 KAGGVGMILANGVFDGEGLVADCHVLPATAVGAASGDEIRKYIEVASKSKSPATATVIFK 603

Query: 488 GTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTE 547
           GTRL VRPAPVVASFSARGPNP SPEI+KPDVIAPGLNILAAWPD IGPSG+ +DKR TE
Sbjct: 604 GTRLRVRPAPVVASFSARGPNPVSPEILKPDVIAPGLNILAAWPDNIGPSGVASDKRKTE 663

Query: 548 FNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTS 607
           FNILSGTSMACPHVSGLAALLKAAHP WSPAAI+SALMTTAYT+DNRGETMLDES+GNTS
Sbjct: 664 FNILSGTSMACPHVSGLAALLKAAHPEWSPAAIRSALMTTAYTVDNRGETMLDESTGNTS 723

Query: 608 TVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAG 667
           TV+D+GAGHVHPQKAMDPGLIYDL  YDYVDFLCNSNYTT NIQV+T K ADCSGAKRAG
Sbjct: 724 TVMDYGAGHVHPQKAMDPGLIYDLTPYDYVDFLCNSNYTTANIQVVTRKKADCSGAKRAG 783

Query: 668 HTGNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLA 727
           H+GNLNYPSL+V FQQYGK KMSTHFIR+VTNVGDA S+Y+V+++PP G+SVTV PEKLA
Sbjct: 784 HSGNLNYPSLSVTFQQYGKPKMSTHFIRSVTNVGDAESVYRVSVRPPKGVSVTVRPEKLA 843

Query: 728 FRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPL 777
           FRR+GQKL+FLVRV+  A +LSPG SS++SGS+IW+DGKH VTSPLVVT+QQPL
Sbjct: 844 FRRIGQKLNFLVRVEVRAAKLSPGGSSLRSGSVIWSDGKHTVTSPLVVTLQQPL 897

BLAST of CSPI07G22140 vs. TAIR10
Match: AT3G14240.1 (AT3G14240.1 Subtilase family protein)

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 591/779 (75.87%), Postives = 692/779 (88.83%), Query Frame = 1

Query: 1   MAVLLRAFFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSV 60
           MA     FFL +L  P SS+SS  ++  T+IV V  ++KPSIFPTH +WY SSLAS++S 
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASS--SNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTS- 60

Query: 61  NDVGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTS 120
               +IIHTY+T+FHGFSA+L+  +  +L   PHV S+IPEQVRH HTTRSPEFLGL+++
Sbjct: 61  -SPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRST 120

Query: 121 DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNR 180
           D AGLL+ESDFGSDLVIGVIDTG+WPER SF+DR LGPVP KWKGQC+ ++DFP ++CNR
Sbjct: 121 DKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNR 180

Query: 181 KLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKA 240
           KL+GARFFC GYEATNGKMNETTE+RSPRDSDGHGTHTASI+AGRYVFPASTLGYA G A
Sbjct: 181 KLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVA 240

Query: 241 AGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA 300
           AGMAPKARLAAYKVCWN+GCYDSDILAAFD AV+DGVDV+SLSVGGVVVPYYLDAIAIGA
Sbjct: 241 AGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGA 300

Query: 301 YRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSV 360
           + A+  G+FVSASAGNGGPG LTVTNVAPW+TTVGAGT+DRDFPA+VKLGNG+++ G SV
Sbjct: 301 FGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSV 360

Query: 361 YGGPALIPGRLYPLIYAGT-EGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGE 420
           YGGP L PGR+YPL+Y G+  GGDGYSSSLCLEGSL+PNLVKGKIVLCDRGINSRA KGE
Sbjct: 361 YGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGE 420

Query: 421 VVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSH--LQPTA 480
           +V+K GGLGMI+ANGVFDGEGLVADCHVLPAT+VGASGGDEIR+YI+E++KS     PTA
Sbjct: 421 IVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTA 480

Query: 481 TILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTD 540
           TI+FKGTRLG+RPAPVVASFSARGPNPE+PEI+KPDVIAPGLNILAAWPD+IGPSG+ +D
Sbjct: 481 TIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSD 540

Query: 541 KRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDES 600
            R TEFNILSGTSMACPHVSGLAALLKAAHP WSPAAI+SAL+TTAYT+DN GE M+DES
Sbjct: 541 NRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDES 600

Query: 601 SGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSG 660
           +GNTS+V+D+G+GHVHP KAMDPGL+YD+ +YDY++FLCNSNYT  NI  IT + ADC G
Sbjct: 601 TGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDG 660

Query: 661 AKRAGHTGNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVE 720
           A+RAGH GNLNYPS +VVFQQYG+ KMSTHFIRTVTNVGD++S+Y++ I+PP G +VTVE
Sbjct: 661 ARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVE 720

Query: 721 PEKLAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPL 777
           PEKL+FRRVGQKLSF+VRV+   V+LSPG++++++G I+W+DGK  VTSPLVVT+QQPL
Sbjct: 721 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

BLAST of CSPI07G22140 vs. TAIR10
Match: AT4G34980.1 (AT4G34980.1 subtilisin-like serine protease 2)

HSP 1 Score: 806.6 bits (2082), Expect = 1.3e-233
Identity = 418/773 (54.08%), Postives = 547/773 (70.76%), Query Frame = 1

Query: 10  LFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSVNDVGAIIHT 69
           L  L  PF S ++  A+K TFI ++   S PSIFPTH +WY +  A  S       I+H 
Sbjct: 9   LLFLSFPFISFAASQAAK-TFIFRIDGGSMPSIFPTHYHWYSTEFAEES------RIVHV 68

Query: 70  YETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTSDSAGLLKES 129
           Y T+FHGFSA ++P E + L+  P V ++  ++ R  HTTRSP+FLGL+  +  GL  ES
Sbjct: 69  YHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ--NQKGLWSES 128

Query: 130 DFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNRKLIGARFFC 189
           D+GSD++IGV DTGIWPER+SF+D +LGP+P +W+G C     F   +CNRK+IGARFF 
Sbjct: 129 DYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFA 188

Query: 190 SGYE-ATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKAR 249
            G + A  G +N+T E+ SPRD+DGHGTHT+S AAGR+ F AS  GYA G A G+APKAR
Sbjct: 189 KGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKAR 248

Query: 250 LAAYKVCW-NAGCYDSDILAAFDAAVSDGVDVVSLSVG---GVVVPYYLDAIAIGAYRAV 309
           +AAYKVCW ++GC DSDILAAFDAAV DGVDV+S+S+G   G+  PYYLD IAIG+Y A 
Sbjct: 249 IAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAA 308

Query: 310 AAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGP 369
           + G+FVS+SAGN GP G++VTN+APWVTTVGA T+DR+FPAD  LG+G  + G S+Y G 
Sbjct: 309 SKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGV 368

Query: 370 ALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKA 429
            L  GR++P++Y G  G    S+SLC+E +L+P  V+GKIV+CDRG + R AKG VVKKA
Sbjct: 369 PL-NGRMFPVVYPGKSGMS--SASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKA 428

Query: 430 GGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGT 489
           GG+GMILANG  +GEGLV D H++PA AVG++ GD I+ Y    A SH  P A+I F+GT
Sbjct: 429 GGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAY----ASSHPNPIASIDFRGT 488

Query: 490 RLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFN 549
            +G++PAPV+ASFS RGPN  SPEI+KPD+IAPG+NILAAW D +GP+G+P+D R TEFN
Sbjct: 489 IVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFN 548

Query: 550 ILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTV 609
           ILSGTSMACPHVSG AALLK+AHP WSPA I+SA+MTT   +DN   +++DES+G ++T 
Sbjct: 549 ILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATP 608

Query: 610 LDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHT 669
            D+G+GH++  +AM+PGL+YD+   DY+ FLC+  Y  K IQVIT     C   ++    
Sbjct: 609 YDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPS-P 668

Query: 670 GNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFR 729
           GNLNYPS+  VF    +  +S   IRT TNVG A ++Y+  I+ P G++VTV+P +L F 
Sbjct: 669 GNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFT 728

Query: 730 RVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTD-GKHEVTSPLVVTMQQPL 777
              ++ S+ V V      +  G +    GS+ W D GKH V SP+VVT    L
Sbjct: 729 SAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of CSPI07G22140 vs. TAIR10
Match: AT5G67360.1 (AT5G67360.1 Subtilase family protein)

HSP 1 Score: 745.0 bits (1922), Expect = 4.8e-215
Identity = 399/765 (52.16%), Postives = 513/765 (67.06%), Query Frame = 1

Query: 7   AFFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSVNDVGAI 66
           AFFL  L + F   SS  + + T+IV + K   PS F  H NWY+SSL SIS   D   +
Sbjct: 10  AFFLL-LCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSIS---DSAEL 69

Query: 67  IHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTSDSAGLL 126
           ++TYE   HGFS +L+  E + L T P V S++PE     HTTR+P FLGL    +A L 
Sbjct: 70  LYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD-EHTADLF 129

Query: 127 KESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNRKLIGAR 186
            E+   SD+V+GV+DTG+WPE +S++D   GP+PS WKG C    +F A+ CNRKLIGAR
Sbjct: 130 PEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGAR 189

Query: 187 FFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPK 246
           FF  GYE+T G ++E+ E RSPRD DGHGTHT+S AAG  V  AS LGYA G A GMAP+
Sbjct: 190 FFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPR 249

Query: 247 ARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAA 306
           AR+A YKVCW  GC+ SDILAA D A++D V+V+S+S+GG +  YY D +AIGA+ A+  
Sbjct: 250 ARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMER 309

Query: 307 GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPAL 366
           G+ VS SAGN GP   +++NVAPW+TTVGAGT+DRDFPA   LGNG+   G S++ G AL
Sbjct: 310 GILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEAL 369

Query: 367 IPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGG 426
            P +L P IYAG    +  + +LC+ G+L P  VKGKIV+CDRGIN+R  KG+VVK AGG
Sbjct: 370 -PDKLLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGG 429

Query: 427 LGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRL 486
           +GMILAN   +GE LVAD H+LPAT VG   GD IR Y+     +   PTA+I   GT +
Sbjct: 430 VGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVT----TDPNPTASISILGTVV 489

Query: 487 GVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNIL 546
           GV+P+PVVA+FS+RGPN  +P I+KPD+IAPG+NILAAW    GP+G+ +D R  EFNI+
Sbjct: 490 GVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNII 549

Query: 547 SGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLD 606
           SGTSM+CPHVSGLAALLK+ HP WSPAAI+SALMTTAY     G+ +LD ++G  ST  D
Sbjct: 550 SGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFD 609

Query: 607 FGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGN 666
            GAGHV P  A +PGLIYDL T DY+ FLC  NYT+  I+ ++ +   C  +K +    +
Sbjct: 610 HGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSK-SYSVAD 669

Query: 667 LNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRV 726
           LNYPS AV     G +K    + RTVT+VG A +         +G+ ++VEP  L F+  
Sbjct: 670 LNYPSFAVNVDGVGAYK----YTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEA 729

Query: 727 GQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVT 772
            +K S+ V      V  S  S S   GSI W+DGKH V SP+ ++
Sbjct: 730 NEKKSYTV---TFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of CSPI07G22140 vs. TAIR10
Match: AT3G14067.1 (AT3G14067.1 Subtilase family protein)

HSP 1 Score: 716.5 bits (1848), Expect = 1.8e-206
Identity = 393/777 (50.58%), Postives = 507/777 (65.25%), Query Frame = 1

Query: 8   FFLFSLLIPF-SSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSVNDVGAI 67
           FF+F LL+ F S SSS     +++IV V +  KPS+F +H NW+ S L S+ S      +
Sbjct: 10  FFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATL 69

Query: 68  IHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTSDSAGLL 127
           +++Y    HGFSA+LSP++   L+  P V S+IP+Q R  HTT +P FLG   S ++GL 
Sbjct: 70  LYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF--SQNSGLW 129

Query: 128 KESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNRKLIGAR 187
             S++G D+++GV+DTGIWPE  SF+D  LGP+PS WKG+C +  DFPA+SCNRKLIGAR
Sbjct: 130 SNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGAR 189

Query: 188 FFCSGY--EATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMA 247
            F  GY  +    K +   E RSPRD++GHGTHTAS AAG  V  AS   YARG A GMA
Sbjct: 190 AFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMA 249

Query: 248 PKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVG--GVVVPYYLDAIAIGAYR 307
            KAR+AAYK+CW  GCYDSDILAA D AV+DGV V+SLSVG  G    Y+ D+IAIGA+ 
Sbjct: 250 SKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFG 309

Query: 308 AVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYG 367
           A   G+ VS SAGN GP   T TN+APW+ TVGA T+DR+F A+   G+G+V  GTS+Y 
Sbjct: 310 ATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYA 369

Query: 368 GPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVK 427
           G +L P     L+Y+G  G     S LC  G LN +LV+GKIVLCDRG N+R  KG  VK
Sbjct: 370 GESL-PDSQLSLVYSGDCG-----SRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVK 429

Query: 428 KAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFK 487
            AGG GMILAN    GE L AD H++PAT VGA  GD+IR YI    K+   PTA I F 
Sbjct: 430 LAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYI----KTSDSPTAKISFL 489

Query: 488 GTRLGVRP-APVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTT 547
           GT +G  P +P VA+FS+RGPN  +P I+KPDVIAPG+NILA W   +GP+ +  D R  
Sbjct: 490 GTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRV 549

Query: 548 EFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNT 607
           +FNI+SGTSM+CPHVSGLAALL+ AHP WSPAAIKSAL+TTAY ++N GE + D ++G +
Sbjct: 550 QFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKS 609

Query: 608 STVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVI--TGKIADCSGAK 667
           S     GAGHV P KA++PGL+YD+   +YV FLC   Y    I V      + D     
Sbjct: 610 SNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETS 669

Query: 668 RAGHTGNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVG-DANSIYKVTIKPPSGISVTVEP 727
           +    G+LNYPS +VVF   G+      + R V NVG + +++Y+V +K P+ + + V P
Sbjct: 670 KLRTAGDLNYPSFSVVFASTGE---VVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSP 729

Query: 728 EKLAFRRVGQKLSFLVRVQAMAVRLSPGS-SSMKSGSIIWTDGKHEVTSPLVVTMQQ 775
            KLAF +    L + V  +++ +    GS    + GSI WTDG+H V SP+ V   Q
Sbjct: 730 SKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQ 771

BLAST of CSPI07G22140 vs. TAIR10
Match: AT2G05920.1 (AT2G05920.1 Subtilase family protein)

HSP 1 Score: 714.9 bits (1844), Expect = 5.3e-206
Identity = 384/746 (51.47%), Postives = 504/746 (67.56%), Query Frame = 1

Query: 26  SKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSVNDVGAIIHTYETLFHGFSAKLSPLE 85
           +KKT+I++V+   KP  F TH +WY S L S SS      +++TY T FHGFSA L   E
Sbjct: 26  AKKTYIIRVNHSDKPESFLTHHDWYTSQLNSESS------LLYTYTTSFHGFSAYLDSTE 85

Query: 86  VEKLQTLPH-VASIIPEQVRHPHTTRSPEFLGLKTSDSAGLLKESDFGSDLVIGVIDTGI 145
            + L +  + +  I  + +   HTTR+PEFLGL +      L  S  G  ++IGV+DTG+
Sbjct: 86  ADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNG--VIIGVLDTGV 145

Query: 146 WPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNRKLIGARFFCSGYE-ATNGKMNETT 205
           WPE +SF+D D+  +PSKWKG+C    DF +  CN+KLIGAR F  G++ A+ G  +   
Sbjct: 146 WPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKR 205

Query: 206 EYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARLAAYKVCWNAGCYDS 265
           E  SPRD DGHGTHT++ AAG  V  AS LGYA G A GMA +AR+A YKVCW+ GC+ S
Sbjct: 206 ESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGS 265

Query: 266 DILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVFVSASAGNGGPGGLT 325
           DILAA D A+ DGVDV+SLS+GG   PYY D IAIGA+ A+  GVFVS SAGN GP   +
Sbjct: 266 DILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRAS 325

Query: 326 VTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPGRLYPLIYAGTEGGD 385
           V NVAPWV TVGAGT+DRDFPA   LGNG+ + G S+Y G  +    L  L+Y     G+
Sbjct: 326 VANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPL-ELVY---NKGN 385

Query: 386 GYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGMILANGVFDGEGLVA 445
             SS+LCL GSL+ ++V+GKIV+CDRG+N+R  KG VV+ AGGLGMI+AN    GE LVA
Sbjct: 386 SSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVA 445

Query: 446 DCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVRPAPVVASFSARGPN 505
           D H+LPA AVG   GD +R+Y+    KS  +PTA ++FKGT L V+P+PVVA+FS+RGPN
Sbjct: 446 DSHLLPAIAVGKKTGDLLREYV----KSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPN 505

Query: 506 PESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGTSMACPHVSGLAALL 565
             +PEI+KPDVI PG+NILA W D IGP+G+  D R T+FNI+SGTSM+CPH+SGLA LL
Sbjct: 506 TVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLL 565

Query: 566 KAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGAGHVHPQKAMDPGLI 625
           KAAHP WSP+AIKSALMTTAY LDN    + D +  + S     G+GHV PQKA+ PGL+
Sbjct: 566 KAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLV 625

Query: 626 YDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNYPSLAVVFQQYGKHK 685
           YD++T +Y+ FLC+ +YT  +I  I  K    + +K+    G LNYPS +V+F      K
Sbjct: 626 YDISTEEYIRFLCSLDYTVDHIVAIV-KRPSVNCSKKFSDPGQLNYPSFSVLF----GGK 685

Query: 686 MSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQKLSFLVR-VQAMAVR 745
               + R VTNVG A+S+YKVT+     + ++V+P KL+F+ VG+K  + V  V    V 
Sbjct: 686 RVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVS 745

Query: 746 LSPGSSSMKSGSIIWTDGKHEVTSPL 769
           +   ++  + GSI W++ +HEV SP+
Sbjct: 746 M---TNKAEFGSITWSNPQHEVRSPV 747

BLAST of CSPI07G22140 vs. NCBI nr
Match: gi|449447946|ref|XP_004141727.1| (PREDICTED: subtilisin-like protease SBT1.6 [Cucumis sativus])

HSP 1 Score: 1539.2 bits (3984), Expect = 0.0e+00
Identity = 776/777 (99.87%), Postives = 777/777 (100.00%), Query Frame = 1

Query: 1   MAVLLRAFFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSV 60
           MAVLLRAFFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSV
Sbjct: 1   MAVLLRAFFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSV 60

Query: 61  NDVGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTS 120
           NDVGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTS
Sbjct: 61  NDVGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTS 120

Query: 121 DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNR 180
           DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNR
Sbjct: 121 DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNR 180

Query: 181 KLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKA 240
           KLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKA
Sbjct: 181 KLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKA 240

Query: 241 AGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA 300
           AGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA
Sbjct: 241 AGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA 300

Query: 301 YRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSV 360
           YRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSV
Sbjct: 301 YRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSV 360

Query: 361 YGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEV 420
           YGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEV
Sbjct: 361 YGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEV 420

Query: 421 VKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATIL 480
           VKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATIL
Sbjct: 421 VKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATIL 480

Query: 481 FKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRT 540
           FKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRT
Sbjct: 481 FKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRT 540

Query: 541 TEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGN 600
           TEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGN
Sbjct: 541 TEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGN 600

Query: 601 TSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKR 660
           TSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKR
Sbjct: 601 TSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKR 660

Query: 661 AGHTGNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEK 720
           AGH+GNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEK
Sbjct: 661 AGHSGNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEK 720

Query: 721 LAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 778
           LAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ
Sbjct: 721 LAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 777

BLAST of CSPI07G22140 vs. NCBI nr
Match: gi|659124598|ref|XP_008462247.1| (PREDICTED: subtilisin-like protease [Cucumis melo])

HSP 1 Score: 1525.8 bits (3949), Expect = 0.0e+00
Identity = 768/777 (98.84%), Postives = 773/777 (99.49%), Query Frame = 1

Query: 1   MAVLLRAFFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSV 60
           MAVLLRAFFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHK+WYESSLASISSV
Sbjct: 1   MAVLLRAFFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKHWYESSLASISSV 60

Query: 61  NDVGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTS 120
           ND GAIIHTYETLFHGFSAKLSPLEVEKLQTLPH+ASIIPEQVRHPHTTRSPEFLGLKTS
Sbjct: 61  NDGGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHIASIIPEQVRHPHTTRSPEFLGLKTS 120

Query: 121 DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNR 180
           DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPA+SCNR
Sbjct: 121 DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPASSCNR 180

Query: 181 KLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKA 240
           KLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKA
Sbjct: 181 KLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKA 240

Query: 241 AGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA 300
           AGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA
Sbjct: 241 AGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA 300

Query: 301 YRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSV 360
           YRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSV
Sbjct: 301 YRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSV 360

Query: 361 YGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEV 420
           YGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEV
Sbjct: 361 YGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEV 420

Query: 421 VKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATIL 480
           VKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSH  PTATIL
Sbjct: 421 VKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHSPPTATIL 480

Query: 481 FKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRT 540
           FKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRT
Sbjct: 481 FKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRT 540

Query: 541 TEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGN 600
           TEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGN
Sbjct: 541 TEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGN 600

Query: 601 TSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKR 660
           TSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCN+NYTTKNIQVITGKIADCSGAKR
Sbjct: 601 TSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNANYTTKNIQVITGKIADCSGAKR 660

Query: 661 AGHTGNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEK 720
           AGHTGNLNYPSL+VVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEK
Sbjct: 661 AGHTGNLNYPSLSVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEK 720

Query: 721 LAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 778
           LAFRRVGQKLSFLVRVQAMAVRLSPGSSSMK GSIIWTDGKHEVTSPLVVTMQQPLQ
Sbjct: 721 LAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKGGSIIWTDGKHEVTSPLVVTMQQPLQ 777

BLAST of CSPI07G22140 vs. NCBI nr
Match: gi|703074017|ref|XP_010089708.1| (Subtilisin-like protease [Morus notabilis])

HSP 1 Score: 1309.7 bits (3388), Expect = 0.0e+00
Identity = 651/785 (82.93%), Postives = 708/785 (90.19%), Query Frame = 1

Query: 4   LLRAFFLFSLLIPF-----SSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASIS 63
           L  +FFL+  L  F     SS S  D  KKTFIVQV + +KPSIF THKNWYESSL+SIS
Sbjct: 3   LSSSFFLYLFLTIFLSLASSSCSDNDDHKKTFIVQVQRQAKPSIFSTHKNWYESSLSSIS 62

Query: 64  S-------VNDVGAIIHTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRS 123
           S         D   IIHTY+T+F GFSAKL+ LE +KL+TLPHV ++IPEQVR  HTTRS
Sbjct: 63  SSPDNKTTTLDASTIIHTYDTVFDGFSAKLTSLEAQKLRTLPHVLAVIPEQVRRLHTTRS 122

Query: 124 PEFLGLKTSDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAK 183
           PEFLGLK +DSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDL PVPSKWKGQC+  K
Sbjct: 123 PEFLGLKKTDSAGLLKESDFGSDLVIGVIDTGIWPERQSFNDRDLDPVPSKWKGQCVAGK 182

Query: 184 DFPATSCNRKLIGARFFCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPAS 243
           DFPAT CNRKLIGARFFC GYE+TNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPAS
Sbjct: 183 DFPATFCNRKLIGARFFCEGYESTNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPAS 242

Query: 244 TLGYARGKAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPY 303
           TLGYARG AAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAV+DGVDV+SLSVGGVVVPY
Sbjct: 243 TLGYARGVAAGMAPKARLAAYKVCWNAGCYDSDILAAFDAAVADGVDVISLSVGGVVVPY 302

Query: 304 YLDAIAIGAYRAVAAGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN 363
           YLD+IAIGA+ A   GVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN
Sbjct: 303 YLDSIAIGAFGAADNGVFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGN 362

Query: 364 GRVVLGTSVYGGPALIPGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGI 423
           GR + G SVYGGP L PGR+Y LIYAG EG DGYSSSLCLEGSLNP+ VKGKIVLCDRGI
Sbjct: 363 GRTIPGVSVYGGPDLSPGRMYSLIYAGNEGSDGYSSSLCLEGSLNPSSVKGKIVLCDRGI 422

Query: 424 NSRAAKGEVVKKAGGLGMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKS 483
           NSRA KGEVVKKAGG+GMILANGVFDGEGLVADCHVLPAT+VGAS GDEIRKYI  A+KS
Sbjct: 423 NSRATKGEVVKKAGGVGMILANGVFDGEGLVADCHVLPATSVGASSGDEIRKYITSASKS 482

Query: 484 HLQPTATILFKGTRLGVRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGP 543
              PTATILFKGT+LG+RPAPVVASFSARGPNPESPEI+KPDVIAPGLNILAAWPD++GP
Sbjct: 483 RSPPTATILFKGTKLGIRPAPVVASFSARGPNPESPEILKPDVIAPGLNILAAWPDRVGP 542

Query: 544 SGIPTDKRTTEFNILSGTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGE 603
           SGIP+DKR TEFNILSGTSMACPHVSGLAALLKAAHP WSPAAI+SALMTTAYT+DNRGE
Sbjct: 543 SGIPSDKRRTEFNILSGTSMACPHVSGLAALLKAAHPEWSPAAIRSALMTTAYTVDNRGE 602

Query: 604 TMLDESSGNTSTVLDFGAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGK 663
           T+LDES+GN+STV+DFGAGHVHP+KA+DPGL+YD+ +YDYVDFLCNSNYTTKNIQVIT K
Sbjct: 603 TVLDESTGNSSTVMDFGAGHVHPEKAIDPGLVYDITSYDYVDFLCNSNYTTKNIQVITRK 662

Query: 664 IADCSGAKRAGHTGNLNYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSG 723
            ADCSGAK+AGH GNLNYPSL+ +FQQYG+HKMSTHFIRTVTNVGD NS+Y+VTI PPSG
Sbjct: 663 SADCSGAKKAGHAGNLNYPSLSALFQQYGRHKMSTHFIRTVTNVGDPNSVYRVTISPPSG 722

Query: 724 ISVTVEPEKLAFRRVGQKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVT 777
             VTVEPEKLAFRRVGQ+L+FLVRV+A AV+LSPG+S +KSGSI+W+DGKH+VTSPLVVT
Sbjct: 723 TVVTVEPEKLAFRRVGQRLNFLVRVEATAVKLSPGNSYVKSGSIVWSDGKHKVTSPLVVT 782

BLAST of CSPI07G22140 vs. NCBI nr
Match: gi|470140096|ref|XP_004305780.1| (PREDICTED: subtilisin-like protease [Fragaria vesca subsp. vesca])

HSP 1 Score: 1297.7 bits (3357), Expect = 0.0e+00
Identity = 640/769 (83.22%), Postives = 712/769 (92.59%), Query Frame = 1

Query: 8   FFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSVNDVGAII 67
           FF F     F +S+S D+  KTFIVQV  DSKPS+FPTHK+WYESSL+S+SS ++   +I
Sbjct: 9   FFFF-----FLASASSDS--KTFIVQVQPDSKPSVFPTHKHWYESSLSSLSS-DEPTPLI 68

Query: 68  HTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTSDSAGLLK 127
           HTY T+FHGFSAKLSP + +KLQ+LPH+ ++IPEQVR  HTTRSPEFLGL+++D+AGLLK
Sbjct: 69  HTYNTVFHGFSAKLSPSQAQKLQSLPHILALIPEQVRRLHTTRSPEFLGLRSTDTAGLLK 128

Query: 128 ESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNRKLIGARF 187
           ESDFGSDLVIGVIDTG+WPERQSFND DLGPVPSKWKGQC+  ++FPA+SCNRKLIGAR+
Sbjct: 129 ESDFGSDLVIGVIDTGVWPERQSFNDHDLGPVPSKWKGQCVAGENFPASSCNRKLIGARY 188

Query: 188 FCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKA 247
           FC GYE+TNGKMN+TTE+RSPRD+DGHGTHTASIAAGRYVFPASTLGYA+G AAGMAPKA
Sbjct: 189 FCGGYESTNGKMNQTTEFRSPRDTDGHGTHTASIAAGRYVFPASTLGYAKGVAAGMAPKA 248

Query: 248 RLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAG 307
           RLAAYKVCWNAGCYDSDILAAFDAAV+DG DVVSLSVGGVVVPYYLDAIAIGA+ A  AG
Sbjct: 249 RLAAYKVCWNAGCYDSDILAAFDAAVADGCDVVSLSVGGVVVPYYLDAIAIGAFGASDAG 308

Query: 308 VFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALI 367
           VFVSASAGNGGPGGLTVTNVAPWVTTVGAGT+DRDFPADVKLGNGR++ G SVYGGP L 
Sbjct: 309 VFVSASAGNGGPGGLTVTNVAPWVTTVGAGTIDRDFPADVKLGNGRIIPGMSVYGGPGLP 368

Query: 368 PGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGL 427
           PGR++PL+YAG+EGGDGYSSSLCLEGSL+ +LVK KIV+CDRGINSRAAKGEVVKKAGG+
Sbjct: 369 PGRMFPLVYAGSEGGDGYSSSLCLEGSLDKSLVKNKIVVCDRGINSRAAKGEVVKKAGGV 428

Query: 428 GMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLG 487
           GMILANGVFDGEGLVADCHVLPATAV AS GDEIRKYI  AAKS   PTATILFKGTR+ 
Sbjct: 429 GMILANGVFDGEGLVADCHVLPATAVAASTGDEIRKYITAAAKSKSPPTATILFKGTRIR 488

Query: 488 VRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILS 547
           V+PAPVVASFSARGPNPE+PEIVKPDVIAPGLNILAAWPDK+GPSGIP+DKR+TEFNILS
Sbjct: 489 VKPAPVVASFSARGPNPEAPEIVKPDVIAPGLNILAAWPDKVGPSGIPSDKRSTEFNILS 548

Query: 548 GTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDF 607
           GTSMACPHVSGLAALLKAAHP WSPAAI+SALMTTAYT+DNRGETMLDESSGNTSTV+DF
Sbjct: 549 GTSMACPHVSGLAALLKAAHPEWSPAAIRSALMTTAYTVDNRGETMLDESSGNTSTVMDF 608

Query: 608 GAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNL 667
           GAGHVHPQKAMDPGLIYD+++ DYVDFLCNSNYTTKNIQV+T K+A+C+GAKRAGH+GNL
Sbjct: 609 GAGHVHPQKAMDPGLIYDISSSDYVDFLCNSNYTTKNIQVVTRKLANCNGAKRAGHSGNL 668

Query: 668 NYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVG 727
           NYPSL+VVFQQYGK K STHFIRTVTNVGD  S+Y VTI+PP G+SVTV+PEKLAFRRVG
Sbjct: 669 NYPSLSVVFQQYGKRKKSTHFIRTVTNVGDPKSVYHVTIRPPRGMSVTVQPEKLAFRRVG 728

Query: 728 QKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPL 777
           QKL+FLVRVQA  V+LSPGSSSM+SGSI+W+DGKH VTSPLVVTMQQPL
Sbjct: 729 QKLNFLVRVQAREVKLSPGSSSMESGSIMWSDGKHTVTSPLVVTMQQPL 769

BLAST of CSPI07G22140 vs. NCBI nr
Match: gi|590621283|ref|XP_007024750.1| (Subtilase family protein isoform 1 [Theobroma cacao])

HSP 1 Score: 1286.2 bits (3327), Expect = 0.0e+00
Identity = 630/769 (81.92%), Postives = 699/769 (90.90%), Query Frame = 1

Query: 8   FFLFSLLIPFSSSSSIDASKKTFIVQVHKDSKPSIFPTHKNWYESSLASISSVNDVGAII 67
           FF  SLL   SS+S +D  +KTFIV+V  D KPSIF THK+WYESSL+S+ S +    ++
Sbjct: 6   FFFLSLLSLASSASRVD--QKTFIVRVQHDVKPSIFTTHKHWYESSLSSVLSPSTPTQVL 65

Query: 68  HTYETLFHGFSAKLSPLEVEKLQTLPHVASIIPEQVRHPHTTRSPEFLGLKTSDSAGLLK 127
           H Y+ +FHGFSAKLSP E  KLQTLPH+ ++IPEQVRH  TTRSP FLGLKT+DSAGLLK
Sbjct: 66  HVYDNVFHGFSAKLSPTEALKLQTLPHIIAVIPEQVRHVQTTRSPLFLGLKTTDSAGLLK 125

Query: 128 ESDFGSDLVIGVIDTGIWPERQSFNDRDLGPVPSKWKGQCLVAKDFPATSCNRKLIGARF 187
           ESDFGSDLVIGVIDTGIWPERQSFNDRDLGP+PSKWKGQC+  KDF ++SCN+KLIGA+F
Sbjct: 126 ESDFGSDLVIGVIDTGIWPERQSFNDRDLGPIPSKWKGQCVTTKDFGSSSCNKKLIGAKF 185

Query: 188 FCSGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKA 247
           FC+GYEATNGKMNET+E+RSPRDSDGHGTHTASIAAGRYVFPASTLGYA+G AAGMAPKA
Sbjct: 186 FCNGYEATNGKMNETSEFRSPRDSDGHGTHTASIAAGRYVFPASTLGYAKGVAAGMAPKA 245

Query: 248 RLAAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAG 307
           RLAAYKVCWNAGCYDSDILAAFDAAV+DGVDV+SLSVGGVVVPYYLDAIAIGA+ A   G
Sbjct: 246 RLAAYKVCWNAGCYDSDILAAFDAAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAADKG 305

Query: 308 VFVSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALI 367
           +FVSASAGNGGPGGL+VTNVAPWV TVGAGT+DRDFPADVKLGNG+VV G SVY GP L 
Sbjct: 306 IFVSASAGNGGPGGLSVTNVAPWVATVGAGTIDRDFPADVKLGNGKVVPGVSVYNGPGLS 365

Query: 368 PGRLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGL 427
           PGR+YPL+YAGT GGDGYSSSLC+EGSL+P+ VKGK+VLCDRGINSRAAKGEVVKKAGG+
Sbjct: 366 PGRMYPLVYAGTGGGDGYSSSLCMEGSLDPDFVKGKLVLCDRGINSRAAKGEVVKKAGGI 425

Query: 428 GMILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLG 487
           GMILANGVFDGEGLVADCHVLPATAVGA+ GDEIR+YI  A+KS    TATI+FKGTRLG
Sbjct: 426 GMILANGVFDGEGLVADCHVLPATAVGAANGDEIRRYIDSASKSKSPATATIVFKGTRLG 485

Query: 488 VRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILS 547
           VRPAPVVASFSARGPNPE+PEI+KPDVIAPGLNILAAWPDK+GPSG+ +DKR TEFNILS
Sbjct: 486 VRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDKVGPSGVASDKRRTEFNILS 545

Query: 548 GTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDF 607
           GTSMACPHVSGLAALLKAAH  WSPAAIKSALMTTAYT+DNRGETMLDESSGNTSTVLDF
Sbjct: 546 GTSMACPHVSGLAALLKAAHSEWSPAAIKSALMTTAYTVDNRGETMLDESSGNTSTVLDF 605

Query: 608 GAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNL 667
           G+GHVHP KAMDPGL+YD+ + DYVDFLCNSNYT  NIQVIT + ADCSGAKRAGH GNL
Sbjct: 606 GSGHVHPTKAMDPGLVYDITSMDYVDFLCNSNYTINNIQVITRRNADCSGAKRAGHIGNL 665

Query: 668 NYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVG 727
           NYPS + VFQQYGKHKMSTHF+R VTNVGD NS+YKVT++PPSG  VTVEPE+L FRRVG
Sbjct: 666 NYPSFSAVFQQYGKHKMSTHFLRQVTNVGDPNSVYKVTVRPPSGTLVTVEPEQLVFRRVG 725

Query: 728 QKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPL 777
           QKL+FLVRVQA+AV+LSPGS++MKSGSI+W+DGKH VTSPL+VTMQQPL
Sbjct: 726 QKLNFLVRVQAVAVKLSPGSTNMKSGSIVWSDGKHNVTSPLIVTMQQPL 772

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SBT15_ARATH0.0e+0075.87Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana GN=SBT1.5 PE=2 SV=1[more]
SBT16_ARATH2.4e-23254.08Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana GN=SBT1.6 PE=2 SV=1[more]
SBT17_ARATH8.5e-21452.16Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana GN=SBT1.7 PE=1 SV=1[more]
SBT14_ARATH3.2e-20550.58Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana GN=SBT1.4 PE=2 SV=1[more]
SBT18_ARATH9.4e-20551.47Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana GN=SBT1.8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KAP0_CUCSA0.0e+0099.87Uncharacterized protein OS=Cucumis sativus GN=Csa_7G448090 PE=4 SV=1[more]
W9R4Z9_9ROSA0.0e+0082.93Subtilisin-like protease OS=Morus notabilis GN=L484_002479 PE=4 SV=1[more]
A0A061GDY7_THECC0.0e+0081.92Subtilase family protein isoform 1 OS=Theobroma cacao GN=TCM_029233 PE=4 SV=1[more]
F6HXP0_VITVI0.0e+0080.82Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g00450 PE=4 SV=... [more]
A0A059ARI3_EUCGR0.0e+0081.27Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I02156 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14240.10.0e+0075.87 Subtilase family protein[more]
AT4G34980.11.3e-23354.08 subtilisin-like serine protease 2[more]
AT5G67360.14.8e-21552.16 Subtilase family protein[more]
AT3G14067.11.8e-20650.58 Subtilase family protein[more]
AT2G05920.15.3e-20651.47 Subtilase family protein[more]
Match NameE-valueIdentityDescription
gi|449447946|ref|XP_004141727.1|0.0e+0099.87PREDICTED: subtilisin-like protease SBT1.6 [Cucumis sativus][more]
gi|659124598|ref|XP_008462247.1|0.0e+0098.84PREDICTED: subtilisin-like protease [Cucumis melo][more]
gi|703074017|ref|XP_010089708.1|0.0e+0082.93Subtilisin-like protease [Morus notabilis][more]
gi|470140096|ref|XP_004305780.1|0.0e+0083.22PREDICTED: subtilisin-like protease [Fragaria vesca subsp. vesca][more]
gi|590621283|ref|XP_007024750.1|0.0e+0081.92Subtilase family protein isoform 1 [Theobroma cacao][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR003137PA_domain
IPR009020Proteinase propeptide inhibitor
IPR010259S8pro/Inhibitor_I9
IPR015500Peptidase_S8_subtilisin-rel
IPR023828Peptidase_S8_Ser-AS
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G22140.1CSPI07G22140.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 187..339
score: 5.9E-83coord: 94..153
score: 5.9E-83coord: 491..622
score: 5.9
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 132..599
score: 2.7
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 494..622
score: 3.67E-82coord: 107..378
score: 3.67
IPR003137PA domainPFAMPF02225PAcoord: 390..461
score: 1.
IPR009020Proteinase propeptide inhibitorunknownSSF54897Protease propeptides/inhibitorscoord: 28..105
score: 2.
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9GENE3DG3DSA:3.30.70.80coord: 29..93
score: 2.
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 29..105
score: 2.7
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 547..563
score: 2.1E-15coord: 210..223
score: 2.1E-15coord: 132..151
score: 2.1
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 1..775
score:
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 548..558
scor
NoneNo IPR availableGENE3DG3DSA:3.50.30.30coord: 397..446
score: 2.
NoneNo IPR availablePANTHERPTHR10795:SF424SUBTILASE FAMILY PROTEIN-RELATEDcoord: 1..775
score:

The following gene(s) are paralogous to this gene:

None