CSPI07G19820 (gene) Wild cucumber (PI 183967)

NameCSPI07G19820
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
Description4-coumarate--CoA ligase
LocationChr7 : 17339960 .. 17341965 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAATTGTTATCATTTTCTCTTCTCTATAAAATCCCTTCTTCTTTCTATCTATCTACCATCTTCCATCTTCCATCTTCCATCTTCACACCAAACTCATCTCTACTTTTTCTCCTCTCTCTGTTTCTACTCTGTTTTTCTTCAAACCTCAACAATGGCCTTTGAATCAAACGAAACTAATGAATTCATTTTCCGTTCAAAGCTTCCCGATATTCACATTCCCAATCACCTCCCACTCCACGATTATGTTTTCCAAAATCTCTCCAAGTTCGCCTCACGCCCCTGTTTGATCAACGGTGCCACCGGGGATGTTTACACCTACCATGACGTTCAGCTAACAGCCCGTCGAGTCGCCGCTGGCCTACACAACCTCGGTATTAAGAAGGGTGATGTTGTCATGAATTTACTTCCCAATTCCCCGGAGTTTGTCTTCACCTTCCTTGGTGCGTCTTACCGGGGCGCCATCATGACAGCAGCCAATCCTTTTTACACGGCTGTGGAAATTGCTAAACAGGCCAAAGCTGCCAATGCGAAATTGATTGTGACAATGGCTTCTTTTTACGATCGGGTTAAGGATTTGGCTGAAAACGGTGTTCAAGTTGTTTGTGTTGATTTTGCTGTAGAGGGTTGTTTGCATTTTTCTGTTTTGAGTGGGGCTGATGAATCTCTTGCGCCGTTGGTGGATTTTTCCTCTAATGACGTGGTGGCGTTGCCGTATTCCTCCGGCACTACCGGTTTGCCGAAGGGAGTGATGTTGACGCATAAAGGGTTAATCACAAGTGTCGCTCAACAAATGGACGGCGAAAATCCGAACCTCTATTATCATGCCGATGATGTCATCCTCTCTGTGTTGCCGTTTTTTCATATCTATTCACTCAATTCGATTTTGCTGTGTGGACTACGAGTCGGCGCTGCCATTATGATTATGCAAAAATTTGACATCGTTGCACTTTTACAATTGATTGAGAAACATAGAATCTCGATCATGCCCATCGTGCCTCCAATCTTTTTGGCCATCGCTAAGTCCCCAGAATTTGAAAAGTACGACGTGTCGTCCGTGAGGGTTCTAAAATCTGGTGGAGCTCCACTAGGGAAGGAGTTGGAAGATGCCGTGAGGGAGAAATTTCCAACGGCGATTCTCGGACAAGGGTATGGAATGACCGAGGCCGGTCCAGTTCTATCCATGAGCTTAGCTTTTGCGAAAGAACCGTTCCAAGTAAAAGCCGGAGCCTGCGGAACTGTGGTCCGCAATGCAGAGATGAAGATTGTTGACACCGAAACCGGCGCCTCATTGCCGGCGAATTCCTCCGGAGAAATTTGTATTAGAGGTGATCAAATCATGAAAGGATATTTGAATGATTTGGAGTCAACGAAGAGGACTGTTGACAAAGAAGGATGGCTTCACACGGGTGACATCGGCTTCGTGGACGACGATGACGAGCTCTTCATCGTCGACCGACTTAAAGAGCTTATCAAATTCAAGGCCTTTCAAGTGGCTCCTGCTGAACTAGAGGCTCTTCTTATCACACATCCTAAACTATCTGATGCTGCTGTTATTGGGTGAGTTCAAATGAATTTCGCTCTTTGTTTTCGCTAAAAAATATTTGTGGGAGTTTCATTTCTAATCCAATTTTATTTTTCTTATTGTTTTTTAGTATGCCGGATGTAGAGGCGGGAGAAGTGCCGGTGGCGTTTGTGGTGAAGGCGAACGGCGGTGCAATAACCGAGGAAGAAGTAAAGCAATTCATAACAAAACAAGTTGTTTTTTACAAAAGACTAAAACGTGTGTTTTTCGTTAATGCCATTCCAAAAGCTCCTTCCGGCAAAATCCTTAGAAAAGAAATTAGAGCAAAATTAGCTTCCGGTGCTTACAATTAAAACCCCAAATTGCTCCTCTATTATCACCGTTGTAAACTTAGCATTTTTTTCACCTCAAAAATTTAATTTTGTATTCCATTACATTTGATATGTATTATTCCAGAATACTAAATTTAATACCAACTACCA

mRNA sequence

ATGGCCTTTGAATCAAACGAAACTAATGAATTCATTTTCCGTTCAAAGCTTCCCGATATTCACATTCCCAATCACCTCCCACTCCACGATTATGTTTTCCAAAATCTCTCCAAGTTCGCCTCACGCCCCTGTTTGATCAACGGTGCCACCGGGGATGTTTACACCTACCATGACGTTCAGCTAACAGCCCGTCGAGTCGCCGCTGGCCTACACAACCTCGGTATTAAGAAGGGTGATGTTGTCATGAATTTACTTCCCAATTCCCCGGAGTTTGTCTTCACCTTCCTTGGTGCGTCTTACCGGGGCGCCATCATGACAGCAGCCAATCCTTTTTACACGGCTGTGGAAATTGCTAAACAGGCCAAAGCTGCCAATGCGAAATTGATTGTGACAATGGCTTCTTTTTACGATCGGGTTAAGGATTTGGCTGAAAACGGTGTTCAAGTTGTTTGTGTTGATTTTGCTGTAGAGGGTTGTTTGCATTTTTCTGTTTTGAGTGGGGCTGATGAATCTCTTGCGCCGTTGGTGGATTTTTCCTCTAATGACGTGGTGGCGTTGCCGTATTCCTCCGGCACTACCGGTTTGCCGAAGGGAGTGATGTTGACGCATAAAGGGTTAATCACAAGTGTCGCTCAACAAATGGACGGCGAAAATCCGAACCTCTATTATCATGCCGATGATGTCATCCTCTCTGTGTTGCCGTTTTTTCATATCTATTCACTCAATTCGATTTTGCTGTGTGGACTACGAGTCGGCGCTGCCATTATGATTATGCAAAAATTTGACATCGTTGCACTTTTACAATTGATTGAGAAACATAGAATCTCGATCATGCCCATCGTGCCTCCAATCTTTTTGGCCATCGCTAAGTCCCCAGAATTTGAAAAGTACGACGTGTCGTCCGTGAGGGTTCTAAAATCTGGTGGAGCTCCACTAGGGAAGGAGTTGGAAGATGCCGTGAGGGAGAAATTTCCAACGGCGATTCTCGGACAAGGGTATGGAATGACCGAGGCCGGTCCAGTTCTATCCATGAGCTTAGCTTTTGCGAAAGAACCGTTCCAAGTAAAAGCCGGAGCCTGCGGAACTGTGGTCCGCAATGCAGAGATGAAGATTGTTGACACCGAAACCGGCGCCTCATTGCCGGCGAATTCCTCCGGAGAAATTTGTATTAGAGGTGATCAAATCATGAAAGGATATTTGAATGATTTGGAGTCAACGAAGAGGACTGTTGACAAAGAAGGATGGCTTCACACGGGTGACATCGGCTTCGTGGACGACGATGACGAGCTCTTCATCGTCGACCGACTTAAAGAGCTTATCAAATTCAAGGCCTTTCAAGTGGCTCCTGCTGAACTAGAGGCTCTTCTTATCACACATCCTAAACTATCTGATGCTGCTGTTATTGGTATGCCGGATGTAGAGGCGGGAGAAGTGCCGGTGGCGTTTGTGGTGAAGGCGAACGGCGGTGCAATAACCGAGGAAGAAGTAAAGCAATTCATAACAAAACAAGTTGTTTTTTACAAAAGACTAAAACGTGTGTTTTTCGTTAATGCCATTCCAAAAGCTCCTTCCGGCAAAATCCTTAGAAAAGAAATTAGAGCAAAATTAGCTTCCGGTGCTTACAATTAA

Coding sequence (CDS)

ATGGCCTTTGAATCAAACGAAACTAATGAATTCATTTTCCGTTCAAAGCTTCCCGATATTCACATTCCCAATCACCTCCCACTCCACGATTATGTTTTCCAAAATCTCTCCAAGTTCGCCTCACGCCCCTGTTTGATCAACGGTGCCACCGGGGATGTTTACACCTACCATGACGTTCAGCTAACAGCCCGTCGAGTCGCCGCTGGCCTACACAACCTCGGTATTAAGAAGGGTGATGTTGTCATGAATTTACTTCCCAATTCCCCGGAGTTTGTCTTCACCTTCCTTGGTGCGTCTTACCGGGGCGCCATCATGACAGCAGCCAATCCTTTTTACACGGCTGTGGAAATTGCTAAACAGGCCAAAGCTGCCAATGCGAAATTGATTGTGACAATGGCTTCTTTTTACGATCGGGTTAAGGATTTGGCTGAAAACGGTGTTCAAGTTGTTTGTGTTGATTTTGCTGTAGAGGGTTGTTTGCATTTTTCTGTTTTGAGTGGGGCTGATGAATCTCTTGCGCCGTTGGTGGATTTTTCCTCTAATGACGTGGTGGCGTTGCCGTATTCCTCCGGCACTACCGGTTTGCCGAAGGGAGTGATGTTGACGCATAAAGGGTTAATCACAAGTGTCGCTCAACAAATGGACGGCGAAAATCCGAACCTCTATTATCATGCCGATGATGTCATCCTCTCTGTGTTGCCGTTTTTTCATATCTATTCACTCAATTCGATTTTGCTGTGTGGACTACGAGTCGGCGCTGCCATTATGATTATGCAAAAATTTGACATCGTTGCACTTTTACAATTGATTGAGAAACATAGAATCTCGATCATGCCCATCGTGCCTCCAATCTTTTTGGCCATCGCTAAGTCCCCAGAATTTGAAAAGTACGACGTGTCGTCCGTGAGGGTTCTAAAATCTGGTGGAGCTCCACTAGGGAAGGAGTTGGAAGATGCCGTGAGGGAGAAATTTCCAACGGCGATTCTCGGACAAGGGTATGGAATGACCGAGGCCGGTCCAGTTCTATCCATGAGCTTAGCTTTTGCGAAAGAACCGTTCCAAGTAAAAGCCGGAGCCTGCGGAACTGTGGTCCGCAATGCAGAGATGAAGATTGTTGACACCGAAACCGGCGCCTCATTGCCGGCGAATTCCTCCGGAGAAATTTGTATTAGAGGTGATCAAATCATGAAAGGATATTTGAATGATTTGGAGTCAACGAAGAGGACTGTTGACAAAGAAGGATGGCTTCACACGGGTGACATCGGCTTCGTGGACGACGATGACGAGCTCTTCATCGTCGACCGACTTAAAGAGCTTATCAAATTCAAGGCCTTTCAAGTGGCTCCTGCTGAACTAGAGGCTCTTCTTATCACACATCCTAAACTATCTGATGCTGCTGTTATTGGTATGCCGGATGTAGAGGCGGGAGAAGTGCCGGTGGCGTTTGTGGTGAAGGCGAACGGCGGTGCAATAACCGAGGAAGAAGTAAAGCAATTCATAACAAAACAAGTTGTTTTTTACAAAAGACTAAAACGTGTGTTTTTCGTTAATGCCATTCCAAAAGCTCCTTCCGGCAAAATCCTTAGAAAAGAAATTAGAGCAAAATTAGCTTCCGGTGCTTACAATTAA
BLAST of CSPI07G19820 vs. Swiss-Prot
Match: 4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=1 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 1.9e-228
Identity = 388/534 (72.66%), Postives = 460/534 (86.14%), Query Frame = 1

Query: 10  EFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVAAG 69
           + IFRSKLPDI+IPNHLPLH Y F+N+S+F+SRPCLINGA   +YTY DV+L +R+VAAG
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 70  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 129
           LH  GI+  D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA++AK+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 130 VTMASFYDRVKDLA-ENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSSNDVVALPY 189
           VT A   ++VKD A EN V+++C+D A EGCLHFSVL+ A+E   P V+   +DVVALPY
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNLY H++DV+L VLP FHIYSLNS+LLCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 250 LRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSVRVLKSG 309
           LRVGAAI+IMQKFDIV+ L+LI++++++I P VPPI LAIAKSP  + YD+SSVR + SG
Sbjct: 249 LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSG 308

Query: 310 GAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGTVVRNAE 369
            APLGKELED VR KFP A LGQGYGMTEAGPVL+M LAFAKEPF++K+GACGTVVRNAE
Sbjct: 309 AAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAE 368

Query: 370 MKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIGFVDDDD 429
           MKIVD +TG SLP N SGEICIRGDQIMKGYLND E+T RT+DKEGWL+TGDIG++DDDD
Sbjct: 369 MKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDD 428

Query: 430 ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAFVVKANG 489
           ELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M D +AGEVPVAFVV++NG
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNG 488

Query: 490 GAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASGAYN 543
             ITE+EVK FI+KQV+FYKR+KRVFFV+AIPK+PSGKILRK++RAKLA+G  N
Sbjct: 489 STITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of CSPI07G19820 vs. Swiss-Prot
Match: 4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 6.1e-227
Identity = 384/540 (71.11%), Postives = 457/540 (84.63%), Query Frame = 1

Query: 4   ESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTA 63
           E+ ++ + IFRSKLPDI+IP HLPLH Y F+N+S+F+SRPCLINGA   +YTY +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKA 123
           R+VA GL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ANAKLIVTMASFYDRVKDLA-ENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSSND 183
           ++AK+I+T + F  +VKD A EN V+V+C+D A EGCLHFS L+ +DE   P V    +D
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGEN NLY H++DV++ VLP FHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSV 303
           SILLCGLRVGAAI+IMQKFDI   L+LI+K+++SI P VPPI LAIAKSP  + YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGT 363
           R + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIG 423
           VVRNAEMKIVD +TG SLP N  GEICIRGDQIMKGYLND E+T RT+DKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAF 483
           F+D+DDELFIVDRLKELIK+K FQVAPAE+EALL+ HP +SDAAV+ M D +AGEVPVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASGAYN 543
           VV++NG AITE+EVK FI+KQV+FYKR+KRVFFV  +PK+PSGKILRK++RA+LA+G  N
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of CSPI07G19820 vs. Swiss-Prot
Match: 4CL2_SOLTU (4-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=4CL2 PE=3 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 2.8e-224
Identity = 383/543 (70.53%), Postives = 452/543 (83.24%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           M  E+ ++ + IFRSKLPDI+IP HLPLH Y F+NLS+F SRPCLI+GA   +YTY +V+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+R+VA GL+ LGI++ D +M LLPN PEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMASFYDRVKDLA-ENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AKA++AK+++T A F  +VKD A EN ++V+CVD A EGC+HFS L  +DE   P V   
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGEN NLY H+DDV++ VLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+LLC LRVGAAI+IMQKFDI   L+LI KH+++I P VPPI LAIAKSP    YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVD +TG SLP N  GEICIRGDQIMKGYLND E+T RT++KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIGF+DDDDELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAV+ M D +AGEVP
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASG 540
           VAFVV++NG  ITE+EVK FI+KQV+FYKR+KRVFFV  +PK+PSGKILRK++RA+LA+G
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 ISN 545

BLAST of CSPI07G19820 vs. Swiss-Prot
Match: 4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 3.7e-224
Identity = 385/540 (71.30%), Postives = 456/540 (84.44%), Query Frame = 1

Query: 7   ETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRV 66
           +  + IFRSKLPDI+IP +LPLH Y F+N+SKF+SRPCLINGAT +++TY DV+L +RRV
Sbjct: 10  QKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRV 69

Query: 67  AAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANA 126
            +GL  LGIK+GD +M LLPNSPEFVF FLGAS+ G+I T ANPF+T+ E+ KQAKA+NA
Sbjct: 70  GSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAKASNA 129

Query: 127 KLIVTMASFYDRVKDLA-ENGVQVVCVDFAVEG-----CLHFSVLSGADESLAPLVDFSS 186
           KLI+T   + D+VKD A ENGV+++ +D           LHFS L+GADE+  P V+ S 
Sbjct: 130 KLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMPKVEISP 189

Query: 187 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 246
           + VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNLY H+DDV+L VLP FHIYS
Sbjct: 190 DGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLPLFHIYS 249

Query: 247 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 306
           LNS+LLCGLR G+ I+IMQKF+IV  L+LI+K++++I P VPPI LAIAKS   + YD+S
Sbjct: 250 LNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVVDNYDLS 309

Query: 307 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 366
           SVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GAC
Sbjct: 310 SVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGAC 369

Query: 367 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 426
           GTVVRNAEMKIVD ETG+SLP N  GEICIRGDQIMKGYLND E+T RT+DKEGWLHTGD
Sbjct: 370 GTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEGWLHTGD 429

Query: 427 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 486
           IG++DDDDELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVPV
Sbjct: 430 IGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEAAGEVPV 489

Query: 487 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASGA 541
           AFVVK+NG  ITE+E+KQFI+KQV+FYKR+ RVFFV AIPKAPSGKILRK++RA+LA+ A
Sbjct: 490 AFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRARLAAAA 549

BLAST of CSPI07G19820 vs. Swiss-Prot
Match: 4CL1_SOLTU (4-coumarate--CoA ligase 1 OS=Solanum tuberosum GN=4CL1 PE=3 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 8.3e-224
Identity = 382/543 (70.35%), Postives = 452/543 (83.24%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           M  E+ ++ + IFRSKLPDI+IP HLPLH Y F+NLS+F SRPCLI+GA   +YTY +V+
Sbjct: 3   MDTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+R+VA GL+ LGI++ D +M LLPN PEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMASFYDRVKDLA-ENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AKA++AK+++T A F  +VKD A EN ++V+CVD   EGC+HFS L  +DE   P V   
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGEN NLY H+DDV++ VLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+LLC LRVGAAI+IMQKFDI   L+LI KH+++I P VPPI LAIAKSP  + YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL+M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVD +TG SLP N  GEICIRGDQIMKGYLND E+T RT++KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIGF+DDDDELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAV+ M D +AGEVP
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASG 540
           VAFVV++NG  ITE+EVK FI+KQV+FYKR+KRVFFV  +PK+PSGKILRK++RA+LA+G
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 ISN 545

BLAST of CSPI07G19820 vs. TrEMBL
Match: A0A0A0K658_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430250 PE=4 SV=1)

HSP 1 Score: 1054.7 bits (2726), Expect = 3.9e-305
Identity = 535/542 (98.71%), Postives = 539/542 (99.45%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGD YTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMASFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
           AKAANAKLIVTMA FYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240
           NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVGAAI+IMQKFDIV+LLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP A+LGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASGA 540
           AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKE+RAKLASGA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CSPI07G19820 vs. TrEMBL
Match: A0A0A0K8H9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430240 PE=4 SV=1)

HSP 1 Score: 1047.3 bits (2707), Expect = 6.2e-303
Identity = 530/542 (97.79%), Postives = 538/542 (99.26%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMASFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
           AKAANAKLIVTMA FYDRVKDLAENGVQ+VCVDFAVEGCLHFSVLSGADESLAPLVDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240
           NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDG+NPNLYYH +DVIL VLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVDDD+ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASGA 540
           AFV+KANGGAI+EEEVKQFI KQVVFYKRLKRVFFVNAIPKAPSGKILRKE+RAKLASGA
Sbjct: 481 AFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CSPI07G19820 vs. TrEMBL
Match: A0A0A0K6Q5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430230 PE=4 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 5.3e-278
Identity = 488/545 (89.54%), Postives = 511/545 (93.76%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESN+TNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMASFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
           AKAANAKLIVTMA FYDRVKDL ENGV++VCVDF+ EGCLHFSVLSGADESL   VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  DDVIL VLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLR GAAIMIMQKFDIV+L QLI K++ISI+PIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKEL +AV+ KFP AIL QGYGMTEAGPVL+MSLAFAKEPFQVK  AC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKFEAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVD ETG SLP NS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGVSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGF+DD++ELFI+DRLKELIKFK FQVAPAELEALLITHPKL DAAVIGMPDVEAGEVPV
Sbjct: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGA---ITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLA 540
           AFVV+   GA    TEEEVK+FI KQV+FYKRLKRVFFVNAIPKAPSGKILRKEIRA LA
Sbjct: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKRLKRVFFVNAIPKAPSGKILRKEIRANLA 540

Query: 541 SGAYN 543
           SGAYN
Sbjct: 541 SGAYN 545

BLAST of CSPI07G19820 vs. TrEMBL
Match: B8XPP8_9ROSI (4-coumarate coenzyme A ligase OS=Betula luminifera GN=4CL PE=2 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 3.0e-233
Identity = 397/543 (73.11%), Postives = 470/543 (86.56%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MA ++ E  EFIFRSKLPDI+IP HLP+H Y F+N+SK  SRPCLING TG VYTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL  LGI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMASFYDRVKDLA-ENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AK+ANAK++VT A +YDRVKD A ENGV+++C+D   E CLHFS L+ ADE+    VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYANENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNLYYH++DVIL VLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+ LCGLR GA+I+I+ KF+IV+LLQLI+KH++++MPIVPPI LAI K P+ +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSV++LKSGGAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL+M LAFAKEP QVK+GA
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMQVKSGA 360

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVD ETGASL  N  GEICIRGDQIMKGY+ND E+T  T+DKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLARNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG +DDDDELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASG 540
           VAFV ++NG  +TE+E+KQF++KQVVFYKR+ RVFF++ +PK+PSGKILRKE+RAKLA+G
Sbjct: 481 VAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 FSN 542

BLAST of CSPI07G19820 vs. TrEMBL
Match: A0A089YQ16_BETPN (4-coumarate:CoA ligase 1 OS=Betula pendula PE=2 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 8.9e-233
Identity = 395/543 (72.74%), Postives = 470/543 (86.56%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MA ++ E  EFIFRSKLPDI+IP HLP+H Y F+N+SK  SRPCLING TG VYTY+DV+
Sbjct: 1   MAIQAKE-QEFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVE 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTAR+VA+GL  LGI+KGDVVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQ
Sbjct: 61  LTARKVASGLSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQ 120

Query: 121 AKAANAKLIVTMASFYDRVKDLA-ENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFS 180
           AK+ANAK++VT A +YDRVKD   ENGV+++C+D   E CLHFS L+ ADE+    VD S
Sbjct: 121 AKSANAKIVVTQACYYDRVKDYTNENGVKIICIDSPPEDCLHFSELTKADENDVAEVDIS 180

Query: 181 SNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNLYYH++DVIL VLP FHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIY 240

Query: 241 SLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDV 300
           SLNS+ LCGLR GA+I+I+ KF+IV+LLQLI+KH++++MPIVPPI LAI K P+ +KYD+
Sbjct: 241 SLNSVFLCGLRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDL 300

Query: 301 SSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGA 360
           SSV++LKSGGAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL+M LAFAKEP +VK+GA
Sbjct: 301 SSVKMLKSGGAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSGA 360

Query: 361 CGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTG 420
           CGTVVRNAEMKIVD ETGASLP N  GEICIRGDQIMKGY+ND E+T  T+DKEGWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTG 420

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG +DD+DELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  AGEVP
Sbjct: 421 DIGLIDDNDELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVP 480

Query: 481 VAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASG 540
           VAFV ++NG  +TE+E+KQF++KQVVFYKR+ RVFF++ +PK+PSGKILRKE+RAKLA+G
Sbjct: 481 VAFVARSNGSQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAG 540

Query: 541 AYN 543
             N
Sbjct: 541 FSN 542

BLAST of CSPI07G19820 vs. TAIR10
Match: AT3G21240.1 (AT3G21240.1 4-coumarate:CoA ligase 2)

HSP 1 Score: 748.8 bits (1932), Expect = 2.3e-216
Identity = 369/542 (68.08%), Postives = 455/542 (83.95%), Query Frame = 1

Query: 8   TNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVA 67
           +N+ IFRS+LPDI+IPNHLPLHDY+F+N+S+FA++PCLING TG+VYTY DV +T+R++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  AGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAK 127
           AGLHNLG+K+ DVVM LLPNSPE V TFL AS+ GAI T+ANPF+T  EI+KQAKA+ AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMASFYDRVKDLAENGVQVVCVDFAV--EGCLHFSVLSGADESLAPLVD-----FSS 187
           LIVT + + D++K+L  +GV +V  D     E CL FS L+ ++E   P VD      S 
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEE---PRVDSIPEKISP 197

Query: 188 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 247
            DVVALP+SSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNLY++ DDVIL VLP FHIY+
Sbjct: 198 EDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYA 257

Query: 248 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 307
           LNSI+LC LRVGA I+IM KF+I  LL+ I++ ++++  +VPPI LAIAKSPE EKYD+S
Sbjct: 258 LNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLS 317

Query: 308 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 367
           SVR++KSG APLGKELEDA+  KFP A LGQGYGMTEAGPVL+MSL FAKEPF VK+GAC
Sbjct: 318 SVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 377

Query: 368 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 427
           GTVVRNAEMKI+D +TG SLP N  GEICIRG+QIMKGYLND  +T  T+DK+GWLHTGD
Sbjct: 378 GTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGD 437

Query: 428 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 487
           +GF+DDDDELFIVDRLKELIK+K FQVAPAELE+LLI HP+++D AV+ M + +AGEVPV
Sbjct: 438 VGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPV 497

Query: 488 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASGA 543
           AFVV++    I+E+E+KQF++KQVVFYKR+ +VFF ++IPKAPSGKILRK++RA+LA+G 
Sbjct: 498 AFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGL 556

BLAST of CSPI07G19820 vs. TAIR10
Match: AT1G51680.1 (AT1G51680.1 4-coumarate:CoA ligase 1)

HSP 1 Score: 746.9 bits (1927), Expect = 8.8e-216
Identity = 370/544 (68.01%), Postives = 449/544 (82.54%), Query Frame = 1

Query: 5   SNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTAR 64
           +N  ++ IFRSKLPDI+IPNHL LHDY+FQN+S+FA++PCLING TG VYTY DV + +R
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAA 124
           ++AA  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 NAKLIVTMASFYDRVKDLA-ENGVQVVCVDF-----AVEGCLHFSVLSGADESLAPLVD- 184
           N KLI+T A + D++K L  ++GV +VC+D        EGCL F+ L+ +    + ++D 
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 --FSSNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPF 244
              S +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNLY+H+DDVIL VLP 
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFE 304
           FHIY+LNSI+LCGLRVGAAI+IM KF+I  LL+LI++ ++++ P+VPPI LAIAKS E E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 KYDVSSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQV 364
           KYD+SS+RV+KSG APLGKELEDAV  KFP A LGQGYGMTEAGPVL+MSL FAKEPF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KAGACGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGW 424
           K+GACGTVVRNAEMKIVD +TG SL  N  GEICIRG QIMKGYLN+  +T  T+DK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEA 484
           LHTGDIG +DDDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +  A
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 496

Query: 485 GEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAK 540
           GEVPVAFVVK+    ++E++VKQF++KQVVFYKR+ +VFF  +IPKAPSGKILRK++RAK
Sbjct: 497 GEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAK 556

BLAST of CSPI07G19820 vs. TAIR10
Match: AT3G21230.1 (AT3G21230.1 4-coumarate:CoA ligase 5)

HSP 1 Score: 677.6 bits (1747), Expect = 6.6e-195
Identity = 334/546 (61.17%), Postives = 426/546 (78.02%), Query Frame = 1

Query: 4   ESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKF----ASRPCLINGATGDVYTYHDV 63
           E   +++FIFRSKLPDI IPNHLPL DYVFQ  S      +S  C+I+GATG + TY DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAK 123
           Q   RR+AAG+H LGI+ GDVVM LLPNSPEF  +FL  +Y GA+ T ANPFYT  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAANAKLIVTMASFYDRVKDLAENGVQVVCVD---------FAVEGCLHFSVLSGADE 183
           QAKA+ AK+I+T     D++ +L  +GV +VC+D          + +GC+ F+ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 SLAPLVDFSSNDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVIL 243
           +       S  D VA+PYSSGTTGLPKGVM+THKGL+TS+AQ++DGENPNL + A+DVIL
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 SVLPFFHIYSLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAK 303
             LP FHIY+L++++L  +R GAA++I+ +F++  +++LI++++++++P+ PP+ LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SPEFEKYDVSSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAK 363
           SPE E+YD+SSVR++ SG A L KELEDAVR KFP AI GQGYGMTE+G V + SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFQVKAGACGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTV 423
            PF+ K+GACGTV+RNAEMK+VDTETG SLP N SGEIC+RG Q+MKGYLND E+T RT+
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGM 483
           DK+GWLHTGDIGFVDDDDE+FIVDRLKELIKFK +QVAPAELEALLI+HP + DAAV+ M
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 PDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRK 537
            D  A EVPVAFV ++ G  +TE++VK ++ KQVV YKR+K VFF+  IPKA SGKILRK
Sbjct: 500 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 559

BLAST of CSPI07G19820 vs. TAIR10
Match: AT1G65060.1 (AT1G65060.1 4-coumarate:CoA ligase 3)

HSP 1 Score: 670.2 bits (1728), Expect = 1.0e-192
Identity = 334/527 (63.38%), Postives = 413/527 (78.37%), Query Frame = 1

Query: 12  IFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVAAGLH 71
           IFRSKLPDI IPNHLPLH Y F+ LS  + +PCLI G+TG  YTY +  L  RRVA+GL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  NLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLIVT 131
            LGI+KGDV+M LL NS EFVF+F+GAS  GA+ T ANPFYT+ E+ KQ K++ AKLI+T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 MASFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADES--LAPLVDFSSNDVVALPYS 191
            + + D++K+L EN   +   +   E CL FS L   DE+      VD   +D  ALP+S
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFS 213

Query: 192 SGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYSLNSILLCGL 251
           SGTTGLPKGV+LTHK LITSVAQQ+DG+NPNLY  ++DVIL VLP FHIYSLNS+LL  L
Sbjct: 214 SGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSL 273

Query: 252 RVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVSSVRVLKSGG 311
           R GA +++M KF+I ALL LI++HR++I  +VPP+ +A+AK+P    YD+SSVR + SG 
Sbjct: 274 RSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGA 333

Query: 312 APLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGACGTVVRNAEM 371
           APLGKEL+D++R + P AILGQGYGMTEAGPVLSMSL FAKEP   K+G+CGTVVRNAE+
Sbjct: 334 APLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAEL 393

Query: 372 KIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIGFVDDDDE 431
           K+V  ET  SL  N  GEICIRG QIMK YLND E+T  T+D+EGWLHTGDIG+VD+DDE
Sbjct: 394 KVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDE 453

Query: 432 LFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGG 491
           +FIVDRLKE+IKFK FQV PAELE+LLI H  ++DAAV+   D  AGEVPVAFVV++NG 
Sbjct: 454 IFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGN 513

Query: 492 AITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKL 537
            ITEE+VK+++ KQVVFYKRL +VFFV +IPK+PSGKILRK+++AKL
Sbjct: 514 DITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of CSPI07G19820 vs. TAIR10
Match: AT4G05160.1 (AT4G05160.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 402.1 bits (1032), Expect = 5.3e-112
Identity = 222/544 (40.81%), Postives = 335/544 (61.58%), Query Frame = 1

Query: 12  IFRSKLPDIHIPN--HLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVAAG 71
           I+RS  P + +P   +  L  ++F+N S + S+  + +  TGD  T+  ++    R+A G
Sbjct: 11  IYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHG 70

Query: 72  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 131
            H LGI+K DVV+   PNS +F   FL  +  G + T ANP YT  E++KQ K +N K+I
Sbjct: 71  FHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKII 130

Query: 132 VTMASFYDRVKDLAENGVQVVCVDFAV------EGCLHFSVLSGADESLA--PLVDFSSN 191
           +++   +D++K      V +   D            L F  +    E ++  P V+   +
Sbjct: 131 ISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVSEYPFVEIKQS 190

Query: 192 DVVALPYSSGTTGLPKGVMLTH-----KGLITSVAQQMDGENPNLYYHADDVILSVLPFF 251
           D  AL YSSGTTG  KGV LTH       L+ ++ Q + GE     YH   V L  LP F
Sbjct: 191 DTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGE-----YHG--VFLCFLPMF 250

Query: 252 HIYSLNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEK 311
           H++ L  I    L+ G A++ M +F++  +L+ IEK R++ + +VPP+FLA++K    +K
Sbjct: 251 HVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKK 310

Query: 312 YDVSSVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVK 371
           +D+SS++ + SG APLGK+L +      P  +L QGYGMTE   ++S+     ++P   K
Sbjct: 311 FDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSV-----EDPRLGK 370

Query: 372 --AGACGTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEG 431
             +G+ G +    E +IV  ETG S P N  GEI +RG  +MKGYLN+ ++TK T+DK+ 
Sbjct: 371 RNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKS 430

Query: 432 WLHTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVE 491
           W+HTGD+G+ ++D  L++VDR+KELIK+K FQVAPAELE LL++HP + DA VI  PD E
Sbjct: 431 WVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEE 490

Query: 492 AGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRA 539
           AGEVP+AFVV++   +ITE+++++FI KQV  YKRL+RV F++ +PK+ +GKILR+E+  
Sbjct: 491 AGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQ 542

BLAST of CSPI07G19820 vs. NCBI nr
Match: gi|778728828|ref|XP_004135893.2| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus])

HSP 1 Score: 1054.7 bits (2726), Expect = 5.6e-305
Identity = 535/542 (98.71%), Postives = 539/542 (99.45%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGD YTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMASFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
           AKAANAKLIVTMA FYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240
           NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVGAAI+IMQKFDIV+LLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP A+LGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASGA 540
           AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKE+RAKLASGA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CSPI07G19820 vs. NCBI nr
Match: gi|778728825|ref|XP_004135894.2| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus])

HSP 1 Score: 1047.3 bits (2707), Expect = 8.9e-303
Identity = 530/542 (97.79%), Postives = 538/542 (99.26%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMASFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
           AKAANAKLIVTMA FYDRVKDLAENGVQ+VCVDFAVEGCLHFSVLSGADESLAPLVDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240
           NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDG+NPNLYYH +DVIL VLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVDDD+ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASGA 540
           AFV+KANGGAI+EEEVKQFI KQVVFYKRLKRVFFVNAIPKAPSGKILRKE+RAKLASGA
Sbjct: 481 AFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CSPI07G19820 vs. NCBI nr
Match: gi|659122577|ref|XP_008461216.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo])

HSP 1 Score: 1039.3 bits (2686), Expect = 2.4e-300
Identity = 524/542 (96.68%), Postives = 536/542 (98.89%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESN+T EFIFRSKLPDIHIPNHLPLH YVFQN+ KFA+RPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNQTQEFIFRSKLPDIHIPNHLPLHQYVFQNMPKFATRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMASFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
           AKAANAKLIVTMA FYDRVKDLAENGV++VCVDFAVEGCLHFSVLSGADESLAP+VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESLAPVVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVGA I+IMQKFDIV+LLQLIEKH+ISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGATILIMQKFDIVSLLQLIEKHKISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPTA+LGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASGA 540
           AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKE+RAKLA GA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLALGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CSPI07G19820 vs. NCBI nr
Match: gi|659122579|ref|XP_008461217.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 1026.9 bits (2654), Expect = 1.2e-296
Identity = 519/542 (95.76%), Postives = 536/542 (98.89%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVM+LLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMHLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMASFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
           AKAA+AKLIVTMA FYDRVKDL ENGV++VCVDFAVEGCLHFSVLSGADESLAP VDFSS
Sbjct: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDG+NPNLYYH++DVIL VLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLRVG+AI+I+QKFDIV+LLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDTETGASLPANSSGEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVD+D+ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASGA 540
           AFVVKA GGAITEEEVKQFITKQVVFYKRLKRV FVNAIPKAPSGKILRKE+RAKLASGA
Sbjct: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CSPI07G19820 vs. NCBI nr
Match: gi|659122581|ref|XP_008461218.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 971.1 bits (2509), Expect = 8.1e-280
Identity = 488/542 (90.04%), Postives = 514/542 (94.83%), Query Frame = 1

Query: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESN+T+EFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVY+Y+DV 
Sbjct: 1   MAFESNQTHEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYYDVN 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMASFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180
            KAANAKLIVTMA FYDRVKDLAENGV++VCVDF+VEGCLHFSVLSGADESL P VDFSS
Sbjct: 121 VKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFSVEGCLHFSVLSGADESLMPRVDFSS 180

Query: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  DDVIL VLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300
           LNSILLCGLR GAAI IMQKFDIV+L QLI K+++SI+PIVPPIFLAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAITIMQKFDIVSLFQLIGKYKVSIVPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKEL +AV+ KFP AIL QGYGMTEAGPVL+MSLAFAKEPFQVKA AC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAEAC 360

Query: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLP NS GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPENSPGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IGFVDD++ELFI+DRLKELIKFK FQVAPAELEALLI HPKL DAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDNNELFIIDRLKELIKFKTFQVAPAELEALLIAHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKEIRAKLASGA 540
           AFVV+   GA TEEEVKQF+TKQVVFYKRLKRVFFVNAIPKAPSGKILRKE+RAKLASGA
Sbjct: 481 AFVVEEKSGATTEEEVKQFVTKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
4CL2_TOBAC1.9e-22872.664-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=1 SV=1[more]
4CL1_TOBAC6.1e-22771.114-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1[more]
4CL2_SOLTU2.8e-22470.534-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=4CL2 PE=3 SV=1[more]
4CL_VANPL3.7e-22471.304-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1[more]
4CL1_SOLTU8.3e-22470.354-coumarate--CoA ligase 1 OS=Solanum tuberosum GN=4CL1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K658_CUCSA3.9e-30598.71Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430250 PE=4 SV=1[more]
A0A0A0K8H9_CUCSA6.2e-30397.79Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430240 PE=4 SV=1[more]
A0A0A0K6Q5_CUCSA5.3e-27889.54Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430230 PE=4 SV=1[more]
B8XPP8_9ROSI3.0e-23373.114-coumarate coenzyme A ligase OS=Betula luminifera GN=4CL PE=2 SV=1[more]
A0A089YQ16_BETPN8.9e-23372.744-coumarate:CoA ligase 1 OS=Betula pendula PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G21240.12.3e-21668.08 4-coumarate:CoA ligase 2[more]
AT1G51680.18.8e-21668.01 4-coumarate:CoA ligase 1[more]
AT3G21230.16.6e-19561.17 4-coumarate:CoA ligase 5[more]
AT1G65060.11.0e-19263.38 4-coumarate:CoA ligase 3[more]
AT4G05160.15.3e-11240.81 AMP-dependent synthetase and ligase family protein[more]
Match NameE-valueIdentityDescription
gi|778728828|ref|XP_004135893.2|5.6e-30598.71PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus][more]
gi|778728825|ref|XP_004135894.2|8.9e-30397.79PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus][more]
gi|659122577|ref|XP_008461216.1|2.4e-30096.68PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo][more]
gi|659122579|ref|XP_008461217.1|1.2e-29695.76PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo][more]
gi|659122581|ref|XP_008461218.1|8.1e-28090.04PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000873AMP-dep_Synth/Lig
IPR020845AMP-binding_CS
IPR025110AMP-bd_C
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009805 coumarin biosynthetic process
biological_process GO:0009809 lignin biosynthetic process
biological_process GO:0009811 stilbene biosynthetic process
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016207 4-coumarate-CoA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G19820.1CSPI07G19820.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 35..442
score: 2.7E
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 186..197
scor
IPR025110AMP-binding enzyme C-terminal domainPFAMPF13193AMP-binding_Ccoord: 451..526
score: 1.4
NoneNo IPR availableGENE3DG3DSA:2.30.38.10coord: 358..433
score: 3.0
NoneNo IPR availableGENE3DG3DSA:3.30.300.30coord: 439..538
score: 1.6
NoneNo IPR availableGENE3DG3DSA:3.40.50.980coord: 192..347
score: 5.7E-48coord: 28..191
score: 4.4
NoneNo IPR availablePANTHERPTHR24096FAMILY NOT NAMEDcoord: 4..535
score:
NoneNo IPR availablePANTHERPTHR24096:SF1494-COUMARATE--COA LIGASE 1-RELATEDcoord: 4..535
score:
NoneNo IPR availableunknownSSF56801Acetyl-CoA synthetase-likecoord: 10..538
score: 3.92E